Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0002650

Physical Location & Seq

Genomic Coordinates Forward (+)

14393492 .. 14396093

Gene Structure 2602 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1644 bp

ATGGGGCATAGACATCAGTTCACCACATCTCATATTTTTGAGAGTGAAAATGAGCAGAATTGGAATCATATGAACACAGAACAACCTTTTGGGCATTTGGGCTGGGAGGGCACTGGGGAGAACAGTTCTTTCTTTTATCCTGTAGAAAACATGTGTATAGATGGAATGCCTTTTGCTTCAAATTGGAACCCCGCTCCGAGGTCATATGGGTACTCTTCCATGAACCACAATGTTGATACACCGCATTTTCAACCTGATGCTTCTGGTCCATCTCATGACCCTTTTCAGCATCCAATAAGTGCTGGTACCTTTGGCTTGGCCAGTGGGAACTATGCTTATCGTGCACCTTCTTCTAGTTATGACCAGTGTACATTCCTTGGTGTTGAGGGTGGTTTTGTTGATCTCACGATGAGCAACGGAAGGGGTCCTCATAAGAGAAAAAGCCCAGGGATCCCTTCAGTATCTGAGAGAGGAAGCACCAGCAGACATTATAGTGCTGGAAGTAGTTCCTCTGAGCTCCCTAGGTCTGCAGAATTACAGCATGAGAAAGTAAACATGGATACCTCACACATGCCCTGGGATCATGTCTCTATGATCCCCAGCTACAGAGGGAATGGACTCTCAATTAGGGGTGACGGATCCATGAGAAATGTGAGAAGCCGGTCTGAACATGATTTGGAGTCCAATCTAGCTAGGACACATTTATCGAGCAATCCTTTGCATACTTCTTATCCTGCAAGTCTCCCTATTGACCATTCTAGTACCAACGATCTCTCTGGTCAGGGTTCTACTGCTTTGACGAATGAGTGGAACCATATTAGTGTGGCTCCTCATGGAAGGGTAATGGCTCCAGATACAAGTGGTTTTGGTCATGATCCCAACCACTTCCTTTTGGGAAGTAGTGGTAATGCTTCTGTGGATAATGGGTTATACCATCATGATTTCATGTCGAGCAGAAGTACTGCTGTTCCTCAAAGTTATCCTGGTGCCTTAGCCCAAACTGTAAGGGGTGTTCGCAGTACCTATTCTCAAAGATCTCCCTCAGCCTTCAGGGCTTCTTCAAGCAACTTGCGATTGGGACATGCAGCGCATTCAGATGAAGGATTGCAGCCGATGCCAGAAAATTACCCAAGACATCCTAGGCCAGCGGCCTCTGTTGGGTGGCGTCATAATGACAGGAGTGGTAGATTGAGGATGTCTAATAATAGATACCGAGTCCCTGAAGAGTCTGCTCTCCAGGACCGGTTTTCGTCTGAGGGATTTATGGCTGTGGATCGCCCAGTTTTTCATGGGTCGAGGAATATGCTTGATCATCATAGAGACATGAGGCTGGACATAGACAACATGGGTTATGAGGAACTCCTCGCACTAAGCGAAAGGATTGGGAATGTCAGCACAGGCTTGTCTGAAGATTTGATACCGAAGTGTTTAACAGAAACAATATATTGTTCATCGGATCAAATCCAGGATGAAGGTTCATGTGCAATTTGCCTGGAAGAGTACAATGACAGGGATGATGTTGGTGCATTGAAAAGTTGCGGTCACGATTACCATGTGAGCTGCATCAAGAAGTGGTTGTCAATGAAAAATTCTTGTCCAATCTGCAAAGGTTGTGCTCTTCCTGATAATATGAAGGAGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0051865 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03013 ko05164 map03013 map05164

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

548

Amino Acids

60.49

Weight (kDa)

6.15

Isoelectric Point (pI)

53.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	489 - 536	1.1e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	493 - 536	1.9e-13	Ring finger domain
zf-C3HC4	PF00097	494 - 535	1.4e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	494 - 535	2.1e-08	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	494 - 533	1.8e-06	RING-type zinc-finger
zf-RING_11	PF17123	494 - 522	7.5e-06	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002650)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150
fragaria_vesca	FvH4_6g15650 FvH4_6g15650 FvH4_6g15650 FvH4_6g15650
malus_domestica	MD04G1100800.v1.1 MD12G1120500.v1.1
prunus_persica	Prupe.6G238800_v2.0.a1
pyrus_communis	pycom04g09560 pycom12g11950
rosa_chinensis	RchiOBHm_Chr3g0469341
rosa_laevigata	RLG00000024358
rosa_multiflora	Rmu_ssc0000394.1_g000001
rosa_roxburghii	Rroxscaffold_6G00411970
rosa_rugosa	Rorug03G0100500 Rorug03G0100600 Rorug03G0100600 Rorug03G0100700 Rorug03G0100700 Rorug03G0100800 Rorug03G0100800
rosa_samantha	Rh3AG150000 Rh3BG173600 Rh3CG164600 Rh3DG201900
rosa_wichuraiana	Rw3G014140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	305
AccB1I	GGYRCC	2 cut(s)	305, 986
AccBSI	CCGCTC	1 cut(s)	194
AciI	CCGC	4 cut(s)	192, 242, 1148, 1539
AclWI	GGATC	9 cut(s)	445, 458, 588, 589, 633, 646, 869, 1281, 1464
AcoI	YGGCCR	1 cut(s)	318
AcsI	RAATTY	1 cut(s)	1588
AcuI	CTGAAG	4 cut(s)	441, 1033, 1242, 1428
AcyI	GRCGYC	1 cut(s)	1165
AfaI	GTAC	7 cut(s)	212, 307, 370, 763, 961, 1021, 1502
AfiI	CCNNNNNNNGG	2 cut(s)	198, 1158
AflIII	ACRYGT	1 cut(s)	150
AgeI	ACCGGT	1 cut(s)	1242
AgsI	TTSAA	4 cut(s)	180, 251, 1062, 1531
AjnI	CCWGG	6 cut(s)	445, 575, 982, 1236, 1464, 1491
AjuI	GAANNNNNNNTTGG	2 cut(s)	72, 104
AluBI	AGCT	4 cut(s)	517, 603, 692, 1560
AluI	AGCT	4 cut(s)	517, 603, 692, 1560
Alw21I	GWGCWC	3 cut(s)	346, 519, 1618
Alw26I	GTCTC	3 cut(s)	592, 746, 1314
Alw44I	GTGCAC	1 cut(s)	342
AlwI	GGATC	9 cut(s)	445, 458, 588, 589, 633, 646, 869, 1281, 1464
AlwNI	CAGNNNCTG	1 cut(s)	464
AoxI	GGCC	3 cut(s)	318, 1142, 1149
ApaLI	GTGCAC	1 cut(s)	342
ApeKI	GCWGC	3 cut(s)	1085, 1108, 1560
ApoI	RAATTY	1 cut(s)	1588
AsiGI	ACCGGT	1 cut(s)	1242
Asp700I	GAANNNNTTC	1 cut(s)	505
Asp718I	GGTACC	1 cut(s)	305
AspA2I	CCTAGG	2 cut(s)	521, 1139
AspLEI	GCGC	1 cut(s)	1090
AspS9I	GGNCC	3 cut(s)	266, 425, 1240
AsuHPI	GGTGA	2 cut(s)	13, 644
AvaII	GGWCC	3 cut(s)	266, 425, 1240
AvrII	CCTAGG	2 cut(s)	521, 1139
BaeGI	GKGCMC	2 cut(s)	113, 346
BaeI	ACNNNNGTAYC	2 cut(s)	1409, 1442
BalI	TGGCCA	1 cut(s)	320
BamHI	GGATCC	2 cut(s)	450, 638
BanI	GGYRCC	2 cut(s)	305, 986
BanII	GRGCYC	1 cut(s)	519
BarI	GAAGNNNNNNTAC	2 cut(s)	1401, 1433
Bbv12I	GWGCWC	3 cut(s)	346, 519, 1618
BbvCI	CCTCAGC	1 cut(s)	1042
BbvI	GCAGC	3 cut(s)	1097, 1120, 1547
BccI	CCATC	3 cut(s)	155, 277, 942
BcgI	CGANNNNNNTGC	2 cut(s)	1285, 1319
BciT130I	CCWGG	6 cut(s)	447, 577, 984, 1238, 1466, 1493
BciVI	GTATCC	1 cut(s)	553
BclI	TGATCA	1 cut(s)	1309
BcoDI	GTCTC	3 cut(s)	592, 746, 1314
BfaI	CTAG	6 cut(s)	354, 522, 689, 693, 759, 1140
BfmI	CTRYAG	3 cut(s)	141, 528, 604
BfuI	GTATCC	1 cut(s)	553
BglI	GCCNNNNNGGC	1 cut(s)	1052
BglII	AGATCT	1 cut(s)	1034
BisI	GCNGC	4 cut(s)	1086, 1109, 1149, 1561
BlnI	CCTAGG	2 cut(s)	521, 1139
BlsI	GCNGC	4 cut(s)	1087, 1110, 1150, 1562
BmcAI	AGTACT	1 cut(s)	961
Bme1390I	CCNGG	6 cut(s)	447, 577, 984, 1238, 1466, 1493
Bme18I	GGWCC	3 cut(s)	266, 425, 1240
BmgT120I	GGNCC	3 cut(s)	266, 425, 1240
BmiI	GGNNCC	9 cut(s)	188, 307, 426, 452, 640, 812, 828, 849, 988
BmrFI	CCNGG	6 cut(s)	447, 577, 984, 1238, 1466, 1493
BmrI	ACTGGG	2 cut(s)	123, 1274
BmsI	GCATC	4 cut(s)	247, 298, 1104, 1572
BmuI	ACTGGG	2 cut(s)	123, 1274
BpmI	CTGGAG	2 cut(s)	834, 1220
Bpu10I	CCTNAGC	2 cut(s)	991, 1042
BsaHI	GRCGYC	1 cut(s)	1165
BsaJI	CCNNGG	8 cut(s)	197, 376, 445, 446, 521, 575, 576, 1139
BsaWI	WCCGGW	1 cut(s)	1242
Bsc4I	CCNNNNNNNGG	2 cut(s)	198, 1158
Bse118I	RCCGGY	2 cut(s)	660, 1242
Bse1I	ACTGG	4 cut(s)	118, 321, 364, 1280
BseBI	CCWGG	6 cut(s)	447, 577, 984, 1238, 1466, 1493
BseDI	CCNNGG	8 cut(s)	197, 376, 445, 446, 521, 575, 576, 1139
BseGI	GGATG	5 cut(s)	289, 1135, 1200, 1474, 1519
BseLI	CCNNNNNNNGG	2 cut(s)	198, 1158
BseMII	CTCAG	4 cut(s)	456, 504, 1056, 1245
BseNI	ACTGG	4 cut(s)	118, 321, 364, 1280
BseRI	GAGGAG	2 cut(s)	819, 1352
BseSI	GKGCMC	2 cut(s)	113, 346
BseXI	GCAGC	3 cut(s)	1097, 1120, 1547
BseYI	CCCAGC	2 cut(s)	102, 599
BshFI	GGCC	3 cut(s)	320, 1144, 1151
BshNI	GGYRCC	2 cut(s)	305, 986
BshTI	ACCGGT	1 cut(s)	1242
BsiHKAI	GWGCWC	3 cut(s)	346, 519, 1618
BsiSI	CCGG	2 cut(s)	661, 1243
BslFI	GGGAC	2 cut(s)	1092, 1202
BslI	CCNNNNNNNGG	2 cut(s)	198, 1158
BsmAI	GTCTC	3 cut(s)	592, 746, 1314
BsmFI	GGGAC	2 cut(s)	1092, 1202
BsmI	GAATGC	2 cut(s)	171, 1090
BsnI	GGCC	3 cut(s)	320, 1144, 1151
Bsp1286I	GDGCHC	4 cut(s)	113, 346, 519, 1618
Bsp1407I	TGTACA	1 cut(s)	368
BspACI	CCGC	4 cut(s)	192, 242, 1148, 1539
BspANI	GGCC	3 cut(s)	320, 1144, 1151
BspCNI	CTCAG	4 cut(s)	457, 505, 1055, 1246
BspHI	TCATGA	3 cut(s)	274, 871, 937
BspLI	GGNNCC	9 cut(s)	188, 307, 426, 452, 640, 812, 828, 849, 988
BspMAI	CTGCAG	1 cut(s)	532
BspPI	GGATC	9 cut(s)	445, 458, 588, 589, 633, 646, 869, 1281, 1464
BspQI	GCTCTTC	1 cut(s)	1623
BspT107I	GGYRCC	2 cut(s)	305, 986
BsrBI	CCGCTC	1 cut(s)	194
BsrFI	RCCGGY	2 cut(s)	660, 1242
BsrGI	TGTACA	1 cut(s)	368
BsrI	ACTGG	4 cut(s)	118, 321, 364, 1280
BssAI	RCCGGY	2 cut(s)	660, 1242
BssECI	CCNNGG	8 cut(s)	197, 376, 445, 446, 521, 575, 576, 1139
BssNI	GRCGYC	1 cut(s)	1165
BssT1I	CCWWGG	3 cut(s)	376, 521, 1139
Bst2UI	CCWGG	6 cut(s)	447, 577, 984, 1238, 1466, 1493
Bst4CI	ACNGT	2 cut(s)	125, 1003
Bst6I	CTCTTC	4 cut(s)	220, 1218, 1491, 1623
BstACI	GRCGYC	1 cut(s)	1165
BstAPI	GCANNNNNTGC	1 cut(s)	1114
BstAUI	TGTACA	1 cut(s)	368
BstC8I	GCNNGC	1 cut(s)	1146
BstDEI	CTNAG	6 cut(s)	465, 513, 991, 1042, 1254, 1370
BstF5I	GGATG	5 cut(s)	289, 1135, 1200, 1474, 1519
BstHHI	GCGC	1 cut(s)	1090
BstMAI	GTCTC	3 cut(s)	592, 746, 1314
BstMWI	GCNNNNNNNGC	4 cut(s)	108, 341, 1052, 1114
BstNI	CCWGG	6 cut(s)	447, 577, 984, 1238, 1466, 1493
BstNSI	RCATGY	3 cut(s)	154, 574, 1085
BstSCI	CCNGG	6 cut(s)	445, 575, 982, 1236, 1464, 1491
BstSFI	CTRYAG	3 cut(s)	141, 528, 604
BstSLI	GKGCMC	2 cut(s)	113, 346
BstV1I	GCAGC	3 cut(s)	1097, 1120, 1547
BstX2I	RGATCY	3 cut(s)	450, 638, 1034
BstYI	RGATCY	3 cut(s)	450, 638, 1034
BsuI	GTATCC	1 cut(s)	553
BsuRI	GGCC	3 cut(s)	320, 1144, 1151
BtsCI	GGATG	5 cut(s)	289, 1135, 1200, 1474, 1519
BtsIMutI	CAGTG	3 cut(s)	111, 328, 371
Cac8I	GCNNGC	1 cut(s)	1146
CaiI	CAGNNNCTG	1 cut(s)	464
CciI	TCATGA	3 cut(s)	274, 871, 937
CfoI	GCGC	1 cut(s)	1090
Cfr10I	RCCGGY	2 cut(s)	660, 1242
Cfr13I	GGNCC	3 cut(s)	266, 425, 1240
CseI	GACGC	1 cut(s)	1154
Csp6I	GTAC	7 cut(s)	211, 306, 369, 762, 960, 1020, 1501
CspAI	ACCGGT	1 cut(s)	1242
CviQI	GTAC	7 cut(s)	211, 306, 369, 762, 960, 1020, 1501
DdeI	CTNAG	6 cut(s)	465, 513, 991, 1042, 1254, 1370
EaeI	YGGCCR	1 cut(s)	318
Eam1104I	CTCTTC	4 cut(s)	220, 1218, 1491, 1623
EarI	CTCTTC	4 cut(s)	220, 1218, 1491, 1623
Ecl136II	GAGCTC	1 cut(s)	517
Eco130I	CCWWGG	3 cut(s)	376, 521, 1139
Eco24I	GRGCYC	1 cut(s)	519
Eco47I	GGWCC	3 cut(s)	266, 425, 1240
Eco53kI	GAGCTC	1 cut(s)	517
Eco57I	CTGAAG	4 cut(s)	441, 1033, 1242, 1428
EcoICRI	GAGCTC	1 cut(s)	517
EcoO109I	RGGNCCY	1 cut(s)	425
EcoRII	CCWGG	6 cut(s)	445, 575, 982, 1236, 1464, 1491
EcoT14I	CCWWGG	3 cut(s)	376, 521, 1139
EcoT38I	GRGCYC	1 cut(s)	519
ErhI	CCWWGG	3 cut(s)	376, 521, 1139
FaqI	GGGAC	2 cut(s)	1092, 1202
FauI	CCCGC	1 cut(s)	199
FauNDI	CATATG	2 cut(s)	69, 205
FbaI	TGATCA	1 cut(s)	1309
Fnu4HI	GCNGC	4 cut(s)	1086, 1109, 1149, 1561
FokI	GGATG	5 cut(s)	276, 1122, 1207, 1481, 1526
FriOI	GRGCYC	1 cut(s)	519
Fsp4HI	GCNGC	4 cut(s)	1086, 1109, 1149, 1561
FspBI	CTAG	6 cut(s)	354, 522, 689, 693, 759, 1140
GlaI	GCGC	1 cut(s)	1089
GluI	GCNGC	4 cut(s)	1086, 1109, 1149, 1561
GsaI	CCCAGC	2 cut(s)	106, 603
GsuI	CTGGAG	2 cut(s)	834, 1220
HaeIII	GGCC	3 cut(s)	320, 1144, 1151
HapII	CCGG	2 cut(s)	661, 1243
HgaI	GACGC	1 cut(s)	1154
HhaI	GCGC	1 cut(s)	1090
Hin1I	GRCGYC	1 cut(s)	1165
Hin6I	GCGC	1 cut(s)	1088
HinP1I	GCGC	1 cut(s)	1088
HinfI	GANTC	5 cut(s)	64, 618, 680, 1215, 1226
HpaII	CCGG	2 cut(s)	661, 1243
HphI	GGTGA	2 cut(s)	13, 644
Hpy166II	GTNNAC	4 cut(s)	21, 344, 369, 553
Hpy188I	TCNGA	8 cut(s)	198, 466, 514, 667, 1096, 1255, 1408, 1456
Hpy188III	TCNNGA	8 cut(s)	275, 406, 851, 872, 938, 1544, 1567, 1622
Hpy8I	GTNNAC	4 cut(s)	21, 344, 369, 553
HpyAV	CCTTC	8 cut(s)	357, 414, 465, 831, 1057, 1094, 1466, 1627
HpyCH4III	ACNGT	2 cut(s)	125, 1003
HpyF10VI	GCNNNNNNNGC	4 cut(s)	108, 341, 1052, 1114
HpyF3I	CTNAG	6 cut(s)	465, 513, 991, 1042, 1254, 1370
Hsp92I	GRCGYC	1 cut(s)	1165
HspAI	GCGC	1 cut(s)	1088
KpnI	GGTACC	1 cut(s)	309
Ksp22I	TGATCA	1 cut(s)	1309
LguI	GCTCTTC	1 cut(s)	1623
LmnI	GCTCC	4 cut(s)	199, 522, 832, 853
Lsp1109I	GCAGC	3 cut(s)	1097, 1120, 1547
LweI	GCATC	4 cut(s)	247, 298, 1104, 1572
MaeI	CTAG	6 cut(s)	354, 522, 689, 693, 759, 1140
MaeIII	GTNAC	2 cut(s)	632, 1541
MbiI	CCGCTC	1 cut(s)	194
MboII	GAAGA	7 cut(s)	207, 342, 1050, 1235, 1421, 1508, 1610
MflI	RGATCY	3 cut(s)	450, 638, 1034
MhlI	GDGCHC	4 cut(s)	113, 346, 519, 1618
MlsI	TGGCCA	1 cut(s)	320
MluCI	AATT	7 cut(s)	58, 181, 533, 624, 1123, 1485, 1588
MluNI	TGGCCA	1 cut(s)	320
MlyI	GAGTC	4 cut(s)	612, 689, 1224, 1235
Mox20I	TGGCCA	1 cut(s)	320
MroXI	GAANNNNTTC	1 cut(s)	505
MscI	TGGCCA	1 cut(s)	320
MseI	TTAA	1 cut(s)	1430
MslI	CAYNNNNRTG	3 cut(s)	492, 819, 1095
Msp20I	TGGCCA	1 cut(s)	320
MspA1I	CMGCKG	1 cut(s)	1148
MspI	CCGG	2 cut(s)	661, 1243
MspR9I	CCNGG	6 cut(s)	447, 577, 984, 1238, 1466, 1493
Mva1269I	GAATGC	2 cut(s)	171, 1090
MvaI	CCWGG	6 cut(s)	447, 577, 984, 1238, 1466, 1493
MwoI	GCNNNNNNNGC	4 cut(s)	108, 341, 1052, 1114
NdeI	CATATG	2 cut(s)	69, 205
NlaIV	GGNNCC	9 cut(s)	188, 307, 426, 452, 640, 812, 828, 849, 988
NmuCI	GTSAC	2 cut(s)	632, 1541
NspI	RCATGY	3 cut(s)	154, 574, 1085
PagI	TCATGA	3 cut(s)	274, 871, 937
PasI	CCCWGGG	2 cut(s)	446, 576
PciI	ACATGT	1 cut(s)	150
PciSI	GCTCTTC	1 cut(s)	1623
PcsI	WCGNNNNNNNCGW	1 cut(s)	1371
PctI	GAATGC	2 cut(s)	171, 1090
PdmI	GAANNNNTTC	1 cut(s)	505
PfeI	GAWTC	1 cut(s)	64
PfoI	TCCNGGA	2 cut(s)	1236, 1464
PinAI	ACCGGT	1 cut(s)	1242
PkrI	GCNGC	4 cut(s)	1087, 1110, 1150, 1562
PleI	GAGTC	4 cut(s)	612, 688, 1223, 1234
PpsI	GAGTC	4 cut(s)	612, 688, 1223, 1234
PpuMI	RGGWCCY	1 cut(s)	425
PscI	ACATGT	1 cut(s)	150
Psp124BI	GAGCTC	1 cut(s)	519
Psp5II	RGGWCCY	1 cut(s)	425
Psp6I	CCWGG	6 cut(s)	445, 575, 982, 1236, 1464, 1491
PspFI	CCCAGC	2 cut(s)	102, 599
PspGI	CCWGG	6 cut(s)	445, 575, 982, 1236, 1464, 1491
PspN4I	GGNNCC	9 cut(s)	188, 307, 426, 452, 640, 812, 828, 849, 988
PspPI	GGNCC	3 cut(s)	266, 425, 1240
PspPPI	RGGWCCY	1 cut(s)	425
PsrI	GAACNNNNNNTAC	2 cut(s)	996, 1028
PstI	CTGCAG	1 cut(s)	532
PstNI	CAGNNNCTG	1 cut(s)	464
PsuI	RGATCY	3 cut(s)	450, 638, 1034
RsaI	GTAC	7 cut(s)	212, 307, 370, 763, 961, 1021, 1502
RsaNI	GTAC	7 cut(s)	211, 306, 369, 762, 960, 1020, 1501
RseI	CAYNNNNRTG	3 cut(s)	492, 819, 1095
SacI	GAGCTC	1 cut(s)	519
SapI	GCTCTTC	1 cut(s)	1623
SaqAI	TTAA	1 cut(s)	1430
SatI	GCNGC	4 cut(s)	1086, 1109, 1149, 1561
Sau96I	GGNCC	3 cut(s)	266, 425, 1240
ScaI	AGTACT	1 cut(s)	961
SchI	GAGTC	4 cut(s)	612, 689, 1224, 1235
ScrFI	CCNGG	6 cut(s)	447, 577, 984, 1238, 1466, 1493
SduI	GDGCHC	4 cut(s)	113, 346, 519, 1618
SfaNI	GCATC	4 cut(s)	247, 298, 1104, 1572
SfcI	CTRYAG	3 cut(s)	141, 528, 604
SinI	GGWCC	3 cut(s)	266, 425, 1240
SmiMI	CAYNNNNRTG	3 cut(s)	492, 819, 1095
Sse9I	AATT	7 cut(s)	58, 181, 533, 624, 1123, 1485, 1588
SsiI	CCGC	4 cut(s)	192, 242, 1148, 1539
SspMI	CTAG	6 cut(s)	354, 522, 689, 693, 759, 1140
SstI	GAGCTC	1 cut(s)	519
StyD4I	CCNGG	6 cut(s)	445, 575, 982, 1236, 1464, 1491
StyI	CCWWGG	3 cut(s)	376, 521, 1139
TaaI	ACNGT	2 cut(s)	125, 1003
TaqI	TCGA	3 cut(s)	707, 950, 1295
TasI	AATT	7 cut(s)	58, 181, 533, 624, 1123, 1485, 1588
TatI	WGTACW	3 cut(s)	368, 959, 1500
TauI	GCSGC	1 cut(s)	1151
TfiI	GAWTC	1 cut(s)	64
Tru1I	TTAA	1 cut(s)	1430
Tru9I	TTAA	1 cut(s)	1430
TscAI	CASTG	3 cut(s)	118, 328, 371
TseFI	GTSAC	2 cut(s)	632, 1541
TseI	GCWGC	3 cut(s)	1085, 1108, 1560
Tsp45I	GTSAC	2 cut(s)	632, 1541
TspDTI	ATGAA	9 cut(s)	86, 236, 934, 1113, 1277, 1440, 1467, 1485, 1598
TspGWI	ACGGA	2 cut(s)	432, 651
TspRI	CASTG	3 cut(s)	118, 328, 371
VneI	GTGCAC	1 cut(s)	342
VpaK11BI	GGWCC	3 cut(s)	266, 425, 1240
XapI	RAATTY	1 cut(s)	1588
XceI	RCATGY	3 cut(s)	154, 574, 1085
XmaJI	CCTAGG	2 cut(s)	521, 1139
XmnI	GAANNNNTTC	1 cut(s)	505
XspI	CTAG	6 cut(s)	354, 522, 689, 693, 759, 1140
ZrmI	AGTACT	1 cut(s)	961

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.