Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0009425

Physical Location & Seq

Genomic Coordinates Forward (+)

14914327 .. 14920480

Gene Structure 6154 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2652 bp

ATGAGAGAGTTCATAATCCTCAGCTACTTCTCTTCTTCTTCTTCCACCATTGCCACTCTTCCGAATCCCCATGCCGCCAACAACCACCACTCCACCAGACCTTACAAAGACAACACCAACAAACCCTTATCCCTCCCTCCCTCCAAATCCTCCCCTCCAATCTCCACCCTCCATTCCCGCCCCCCTCCCCTCTCCGCCGTCCGCTGGGACCCCAACAAAACTCACCTCTGCTACTTCGCCGACCTCGCCTCAAAACTCGCCCGCGATGGAAGGCTCCAGGATTTCGCCATGATTGTCGAAAGCGTCGTGCTTTCGGGCGTCAAGGGATCAGAGTTCACGGCGGCGCTCAGGGTTGAGCTGGTGGCGAAGGGCATTTCGGGGTTTTTGAAAGAGGGGAAGGTGGGGGGCGTTGTTGATGTGCTGAGTAAGGTTGATGAGCTCGGGGTTCCGCCGTTGAAGCTGTTTGATGGGTACGCTATGGAGTTGCTGGGGAGACAGTGTCGCCGGCTTTTGAAATGTAGGCAAGTGCAGGAACTTGTTGAGCTCATGGAGATTCTTCAAGGTTTTCATTTCCCGATCAAAGAACTGCTGGAGCCATATGAAGTTATCAAAGTTTGTGTTGATAAACGCTGCCCGAAATTGGCAATAAGGTATGCTTGTATTTTTCCACATGCGCATATTTTATTCTGCAACATTATAAATGAATTTGGAAAGAGAAGGGCGTTGGAGTCTGCCTTGACAGCATATGAAGCATCCAAGGAAAACCTGAGTGGTTCTAATATGTATGTCTATCGCACAATAATTGATGTATGTGGCCTCTGCAAAGACTACATGAAATCTAGGTACATCTATGAGGACTTGCTCAAACAGAAGGTCACTCCGAATATTTATGTTTTCAACAGTCTCATGAATGTGAACGCACATGATTTGACCTACACATTCCATGTTTACAGGAATATGAAGAATCTTGGCGTCAGAGCAGATATGGCATGTTATAATATCCTCTTAAAGATATGTTGTCTTGCTGGGCGAGTTGATTTGGCACAGGATATTTACAGAGAAGTACAGCATTTGGAATCAACGGGAGTGTTGAAGTTGGATGTCTTCACGTATAGCACTATTGTAAAGGCAGGTCTTGTTGAGAAGGCAATTCAATTGTTTGAAGAGATGCTTCTGGCTGGCTCTGAACCTAATTCGCAGTGTTTTAACATCCTTCTACATGCTTGCGTCGAGGCTTGCCAGTACGATAGGGCTTTTCGGCTTTTCCAGTCTTTGAAGAGCAAAAACTACAAAGGGCTTTCTTTCAAACCCACAACTACAACGTACAATACTATGATGAAGGCCTGTGGTACTGACTACTACCATGCACAAGCACTAATGGATGAGATGAGAACAATAGGTCTTTATCCTAATCAAATAAGCTGGTCGATTTTGGCTGATATCTGCGGAAGCTCAGGAAATGTCAAGGGTGCTTTGCAGATTTTGACAAATATGCGTGCAGGTGGGATGAAACCTGATGTTGTTGCATATACAACAGCTATTAAGGTCTGTGTGGAAAGTGGAAATTTGGAGCTAGCATTGTCGCTGTTTGCAGAAATGAAAAAATATCAGATACGCCCAAATTTTGTGACATATAATACAATTCTGAGGGCTCGTAGTAGATACGGTTCTGTGAGCGAAGTGCAACAATGCCTGGCTATATACCGGGACATGCGGAATGCGGGTTACAAATCTAATGATCGTTTCCTAGAGCAACTGATTGAGGAGTGGTGTGAAGGAGTTATACAAGACAGCAATGCCAAACAAGGAGAGTTCAGTTCCCACGACAAAACTGACATAGGGAGACCTGGAAGTCTGCTGCTTGAAAAAGTTGCAGAACACTTGCAAAAGCGTACTGCCGAAACCTTAGCAGTTGACCTTCAAGGACTGACAAAGGTTGAAGCTCGGATTGTTCTTCTTGCAGTTCTACGAATGATCAAAGAGAACTACAGCTCAGGGCATTCAGTAAAAGAAGACATGCTGATTGTTGTAGGAGTCCACGATGTTGATGGAGGCTCAACTACACAGAATTTAGAGGTGAAAGATGCAATAACGAAACTTCTGCGGGATGAATTGGGGCTCAAGGTTCTTGCAACAGGACCTAAAACGGGACTTGATACGACAATAGAACCGGCGAACATTACTGATTCAGATCAAGAGTCAGAAGAGATGACAAGTAGGACCGAATTAACGACTGACTTGATATATTCCACCAGACGACCCGTTGCACTAGAAAGGCTAAAGGTAACCAGGGGATCGTTGCTACAGTGGCTGCGGAAAAGAAGTGCCCCTAGAAGTACCGGTAGCTTCAGTTCCAGCTTCACCTTGCTTAAAAAGAGTGGTGGCTACATTTGGCTCCCCCTTTACAGCCAGGATAATACATGGAATTCTATTGTAAAACCGATCCTCAGACAATTGAATAAAAGCAGCATCCTTCTCAACCAACAATATAAAATATGCATCACTCTGCACATCATTCTACATCCACAGCCTCCTGGCCAAATGAAAAATTGGTTACCCTGGCCATTCATTAATCAACTGCAGTCTGCAATAGATAGAAGAAGGGAGACGATTTTCAGCTCGATCGCTCAACCCGAATTTAATCTGCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

884

Amino Acids

99.47

Weight (kDa)

8.86

Isoelectric Point (pI)

41.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	233 - 363	5.7e-06	Pentacotripeptide-repeat region of PRORP
PPR_long	PF17177	265 - 427	9.6e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	367 - 410	1.4e-07	PPR repeat family
PPR_3	PF13812	386 - 450	1.8e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	397 - 449	2.5e-08	PPR repeat family
PPR_long	PF17177	441 - 577	4.9e-10	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	470 - 518	7.6e-06	PPR repeat family
PPR_3	PF13812	494 - 551	2.8e-15	Pentatricopeptide repeat domain
PPR_2	PF13041	505 - 551	2.4e-13	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009425)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02830
fragaria_vesca	FvH4_6g15120 FvH4_6g15120 FvH4_6g15120 FvH4_6g15120 FvH4_6g15120
malus_domestica	MD12G1126500.v1.1
prunus_persica	Prupe.6G243100_v2.0.a1
pyrus_communis	pycom12g12460
rosa_chinensis	RchiOBHm_Chr3g0468421
rosa_laevigata	RLG00000024430
rosa_multiflora	Rmu_sc0014003.1_g000015 Rmu_sc0020046.1_g000004
rosa_roxburghii	Rroxscaffold_6G00412780
rosa_rugosa	Rorug03G0095300 Rorug03G0095400
rosa_samantha	Rh3AG143100 Rh3BG165500 Rh3DG165100
rosa_wichuraiana	Rw0G014180 Rw3G013520 Rw3G013550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	698, 996
AarI	CACCTGC	1 cut(s)	1490
AasI	GACNNNNNNGTC	1 cut(s)	1851
Acc16I	TGCGCA	1 cut(s)	675
Acc36I	ACCTGC	2 cut(s)	1121, 1490
AccII	CGCG	1 cut(s)	264
AclWI	GGATC	3 cut(s)	334, 2302, 2437
AcoI	YGGCCR	2 cut(s)	2536, 2561
AcsI	RAATTY	6 cut(s)	704, 1564, 1621, 2068, 2425, 2636
AcuI	CTGAAG	1 cut(s)	2332
AcyI	GRCGYC	2 cut(s)	318, 972
AfaI	GTAC	8 cut(s)	473, 845, 1065, 1244, 1325, 1351, 1894, 2338
AfiI	CCNNNNNNNGG	3 cut(s)	204, 640, 2147
AgeI	ACCGGT	1 cut(s)	2339
AjnI	CCWGG	7 cut(s)	276, 1692, 1846, 2288, 2409, 2533, 2558
Alw21I	GWGCWC	2 cut(s)	441, 546
Alw26I	GTCTC	4 cut(s)	487, 908, 1837, 2600
AlwI	GGATC	3 cut(s)	334, 2302, 2437
AlwNI	CAGNNNCTG	1 cut(s)	2311
Ama87I	CYCGRG	1 cut(s)	440
AoxI	GGCC	4 cut(s)	814, 1341, 2536, 2561
ApeKI	GCWGC	4 cut(s)	630, 1858, 2311, 2466
ApoI	RAATTY	6 cut(s)	704, 1564, 1621, 2068, 2425, 2636
ArsI	GACNNNNNNTTYG	2 cut(s)	858, 890
AseI	ATTAAT	1 cut(s)	2571
AsiGI	ACCGGT	1 cut(s)	2339
AspLEI	GCGC	2 cut(s)	346, 676
AspS9I	GGNCC	3 cut(s)	208, 2138, 2220
AsuC2I	CCSGG	1 cut(s)	1706
AsuHPI	GGTGA	3 cut(s)	215, 2089, 2353
AsuNHI	GCTAGC	1 cut(s)	1573
AvaI	CYCGRG	1 cut(s)	440
AvaII	GGWCC	3 cut(s)	208, 2138, 2220
BaeGI	GKGCMC	1 cut(s)	2329
BalI	TGGCCA	2 cut(s)	2538, 2563
BanII	GRGCYC	4 cut(s)	441, 546, 1654, 2121
BarI	GAAGNNNNNNTAC	2 cut(s)	1767, 1799
BbsI	GAAGAC	2 cut(s)	1096, 2019
Bbv12I	GWGCWC	2 cut(s)	441, 546
BbvCI	CCTCAGC	1 cut(s)	20
BbvI	GCAGC	4 cut(s)	617, 1845, 2298, 2478
BccI	CCATC	3 cut(s)	260, 461, 2042
BceAI	ACGGC	3 cut(s)	182, 354, 436
BcgI	CGANNNNNNTGC	2 cut(s)	2083, 2117
BciT130I	CCWGG	7 cut(s)	278, 1694, 1848, 2290, 2411, 2535, 2560
BclI	TGATCA	1 cut(s)	1974
BcnI	CCSGG	1 cut(s)	1706
BcoDI	GTCTC	4 cut(s)	487, 908, 1837, 2600
BfaI	CTAG	5 cut(s)	840, 1574, 1748, 2270, 2331
BfmI	CTRYAG	3 cut(s)	1987, 2303, 2579
BfoI	RGCGCY	1 cut(s)	347
BfuAI	ACCTGC	2 cut(s)	1121, 1490
BisI	GCNGC	6 cut(s)	75, 342, 631, 1859, 2312, 2467
BlsI	GCNGC	6 cut(s)	76, 343, 632, 1860, 2313, 2468
Bme1390I	CCNGG	8 cut(s)	278, 1694, 1706, 1848, 2290, 2411, 2535, 2560
Bme18I	GGWCC	3 cut(s)	208, 2138, 2220
BmeT110I	CYCGRG	1 cut(s)	440
BmgT120I	GGNCC	3 cut(s)	208, 2138, 2220
BmiI	GGNNCC	6 cut(s)	209, 210, 275, 447, 594, 2396
BmrFI	CCNGG	8 cut(s)	278, 1694, 1706, 1848, 2290, 2411, 2535, 2560
BmsI	GCATC	5 cut(s)	761, 1158, 2074, 2478, 2508
BmtI	GCTAGC	1 cut(s)	1577
BpiI	GAAGAC	2 cut(s)	1096, 2019
BpmI	CTGGAG	2 cut(s)	260, 611
Bpu10I	CCTNAGC	5 cut(s)	20, 347, 1453, 1906, 1993
BpuEI	CTTGAG	1 cut(s)	2105
BpuMI	CCSGG	1 cut(s)	1706
BsaAI	YACGTR	1 cut(s)	1110
BsaBI	GATNNNNATC	1 cut(s)	2190
BsaHI	GRCGYC	2 cut(s)	318, 972
BsaI	GGTCTC	1 cut(s)	1837
BsaJI	CCNNGG	3 cut(s)	756, 2289, 2558
BsaWI	WCCGGW	1 cut(s)	2339
BsaXI	ACNNNNNCTCC	4 cut(s)	74, 104, 484, 514
Bsc4I	CCNNNNNNNGG	3 cut(s)	204, 640, 2147
Bse118I	RCCGGY	3 cut(s)	504, 2170, 2339
Bse1I	ACTGG	2 cut(s)	1240, 1267
Bse3DI	GCAATG	2 cut(s)	48, 1801
Bse8I	GATNNNNATC	1 cut(s)	2190
BseBI	CCWGG	7 cut(s)	278, 1694, 1848, 2290, 2411, 2535, 2560
BseDI	CCNNGG	3 cut(s)	756, 2289, 2558
BseGI	GGATG	8 cut(s)	752, 1105, 1209, 1387, 1512, 2113, 2469, 2521
BseJI	GATNNNNATC	1 cut(s)	2190
BseLI	CCNNNNNNNGG	3 cut(s)	204, 640, 2147
BseMI	GCAATG	2 cut(s)	48, 1801
BseMII	CTCAG	8 cut(s)	34, 361, 413, 758, 1467, 1637, 2007, 2461
BseNI	ACTGG	2 cut(s)	1240, 1267
BseRI	GAGGAG	1 cut(s)	1778
BseSI	GKGCMC	1 cut(s)	2329
BseXI	GCAGC	4 cut(s)	617, 1845, 2298, 2478
BseYI	CCCAGC	3 cut(s)	204, 487, 1025
BsgI	GTGCAG	3 cut(s)	548, 1518, 2492
Bsh1236I	CGCG	1 cut(s)	264
Bsh1285I	CGRYCG	1 cut(s)	2625
BshFI	GGCC	4 cut(s)	816, 1343, 2538, 2563
BshTI	ACCGGT	1 cut(s)	2339
BsiEI	CGRYCG	1 cut(s)	2625
BsiHKAI	GWGCWC	2 cut(s)	441, 546
BsiHKCI	CYCGRG	1 cut(s)	440
BsiSI	CCGG	4 cut(s)	505, 1705, 2171, 2340
BslFI	GGGAC	3 cut(s)	221, 1721, 2163
BslI	CCNNNNNNNGG	3 cut(s)	204, 640, 2147
BsmAI	GTCTC	4 cut(s)	487, 908, 1837, 2600
BsmBI	CGTCTC	1 cut(s)	2600
BsmFI	GGGAC	3 cut(s)	221, 1721, 2163
BsmI	GAATGC	2 cut(s)	1723, 1999
BsnI	GGCC	4 cut(s)	816, 1343, 2538, 2563
Bso31I	GGTCTC	1 cut(s)	1837
BsoBI	CYCGRG	1 cut(s)	440
Bsp1286I	GDGCHC	5 cut(s)	441, 546, 1654, 2121, 2329
Bsp143I	GATC	8 cut(s)	326, 576, 1738, 1974, 2191, 2294, 2442, 2622
BspANI	GGCC	4 cut(s)	816, 1343, 2538, 2563
BspCNI	CTCAG	8 cut(s)	33, 360, 414, 759, 1466, 1638, 2006, 2460
BspFNI	CGCG	1 cut(s)	264
BspHI	TCATGA	1 cut(s)	906
BspLI	GGNNCC	6 cut(s)	209, 210, 275, 447, 594, 2396
BspMAI	CTGCAG	1 cut(s)	2583
BspMI	ACCTGC	2 cut(s)	1121, 1490
BspOI	GCTAGC	1 cut(s)	1577
BspPI	GGATC	3 cut(s)	334, 2302, 2437
BspQI	GCTCTTC	1 cut(s)	1271
BspTNI	GGTCTC	1 cut(s)	1837
BsrDI	GCAATG	2 cut(s)	48, 1801
BsrFI	RCCGGY	3 cut(s)	504, 2170, 2339
BsrI	ACTGG	2 cut(s)	1240, 1267
BssAI	RCCGGY	3 cut(s)	504, 2170, 2339
BssECI	CCNNGG	3 cut(s)	756, 2289, 2558
BssMI	GATC	8 cut(s)	326, 576, 1738, 1974, 2191, 2294, 2442, 2622
BssNI	GRCGYC	2 cut(s)	318, 972
BssT1I	CCWWGG	1 cut(s)	756
Bst2UI	CCWGG	7 cut(s)	278, 1694, 1848, 2290, 2411, 2535, 2560
Bst4CI	ACNGT	4 cut(s)	498, 902, 1667, 2307
Bst6I	CTCTTC	5 cut(s)	37, 63, 1158, 1271, 2199
BstACI	GRCGYC	2 cut(s)	318, 972
BstBAI	YACGTR	1 cut(s)	1110
BstC8I	GCNNGC	7 cut(s)	262, 506, 1180, 1225, 1237, 1497, 1575
BstDEI	CTNAG	9 cut(s)	20, 347, 422, 767, 1453, 1646, 1906, 1993, 2447
BstEII	GGTNACC	2 cut(s)	2284, 2553
BstF5I	GGATG	8 cut(s)	752, 1105, 1209, 1387, 1512, 2113, 2469, 2521
BstFNI	CGCG	1 cut(s)	264
BstH2I	RGCGCY	1 cut(s)	347
BstHHI	GCGC	2 cut(s)	346, 676
BstKTI	GATC	8 cut(s)	329, 579, 1741, 1977, 2194, 2297, 2445, 2625
BstMAI	GTCTC	4 cut(s)	487, 908, 1837, 2600
BstMBI	GATC	8 cut(s)	326, 576, 1738, 1974, 2191, 2294, 2442, 2622
BstMCI	CGRYCG	1 cut(s)	2625
BstMWI	GCNNNNNNNGC	6 cut(s)	245, 457, 740, 749, 986, 2308
BstNI	CCWGG	7 cut(s)	278, 1694, 1848, 2290, 2411, 2535, 2560
BstNSI	RCATGY	5 cut(s)	674, 993, 1223, 1714, 2020
BstPI	GGTNACC	2 cut(s)	2284, 2553
BstSCI	CCNGG	8 cut(s)	276, 1692, 1704, 1846, 2288, 2409, 2533, 2558
BstSFI	CTRYAG	3 cut(s)	1987, 2303, 2579
BstSLI	GKGCMC	1 cut(s)	2329
BstUI	CGCG	1 cut(s)	264
BstV1I	GCAGC	4 cut(s)	617, 1845, 2298, 2478
BstV2I	GAAGAC	2 cut(s)	1096, 2019
BsuRI	GGCC	4 cut(s)	816, 1343, 2538, 2563
BtgZI	GCGATG	1 cut(s)	279
BtsCI	GGATG	8 cut(s)	752, 1105, 1209, 1387, 1512, 2113, 2469, 2521
BtsI	GCAGTG	1 cut(s)	1205
BtsIMutI	CAGTG	3 cut(s)	503, 1205, 2312
BveI	ACCTGC	2 cut(s)	1121, 1490
Cac8I	GCNNGC	7 cut(s)	262, 506, 1180, 1225, 1237, 1497, 1575
CaiI	CAGNNNCTG	1 cut(s)	2311
CciI	TCATGA	1 cut(s)	906
CfoI	GCGC	2 cut(s)	346, 676
Cfr10I	RCCGGY	3 cut(s)	504, 2170, 2339
Cfr13I	GGNCC	3 cut(s)	208, 2138, 2220
CseI	GACGC	4 cut(s)	292, 307, 961, 1216
Csp6I	GTAC	8 cut(s)	472, 844, 1064, 1243, 1324, 1350, 1893, 2337
CspAI	ACCGGT	1 cut(s)	2339
CviQI	GTAC	8 cut(s)	472, 844, 1064, 1243, 1324, 1350, 1893, 2337
DdeI	CTNAG	9 cut(s)	20, 347, 422, 767, 1453, 1646, 1906, 1993, 2447
DpnI	GATC	8 cut(s)	328, 578, 1740, 1976, 2193, 2296, 2444, 2624
DpnII	GATC	8 cut(s)	326, 576, 1738, 1974, 2191, 2294, 2442, 2622
DrdI	GACNNNNNNGTC	1 cut(s)	1851
DseDI	GACNNNNNNGTC	1 cut(s)	1851
EaeI	YGGCCR	2 cut(s)	2536, 2561
Eam1104I	CTCTTC	5 cut(s)	37, 63, 1158, 1271, 2199
EarI	CTCTTC	5 cut(s)	37, 63, 1158, 1271, 2199
EciI	GGCGGA	2 cut(s)	184, 438
Ecl136II	GAGCTC	2 cut(s)	439, 544
Eco130I	CCWWGG	1 cut(s)	756
Eco147I	AGGCCT	1 cut(s)	1343
Eco24I	GRGCYC	4 cut(s)	441, 546, 1654, 2121
Eco31I	GGTCTC	1 cut(s)	1837
Eco32I	GATATC	1 cut(s)	1441
Eco47I	GGWCC	3 cut(s)	208, 2138, 2220
Eco53kI	GAGCTC	2 cut(s)	439, 544
Eco57I	CTGAAG	1 cut(s)	2332
Eco88I	CYCGRG	1 cut(s)	440
Eco91I	GGTNACC	2 cut(s)	2284, 2553
EcoICRI	GAGCTC	2 cut(s)	439, 544
EcoO109I	RGGNCCY	2 cut(s)	208, 2138
EcoO65I	GGTNACC	2 cut(s)	2284, 2553
EcoRI	GAATTC	1 cut(s)	2425
EcoRII	CCWGG	7 cut(s)	276, 1692, 1846, 2288, 2409, 2533, 2558
EcoRV	GATATC	1 cut(s)	1441
EcoT14I	CCWWGG	1 cut(s)	756
EcoT22I	ATGCAT	1 cut(s)	2501
EcoT38I	GRGCYC	4 cut(s)	441, 546, 1654, 2121
ErhI	CCWWGG	1 cut(s)	756
Esp3I	CGTCTC	1 cut(s)	2600
FalI	AAGNNNNNCTT	4 cut(s)	640, 672, 1155, 1187
FaqI	GGGAC	3 cut(s)	221, 1721, 2163
FauI	CCCGC	4 cut(s)	185, 269, 1714, 2097
FauNDI	CATATG	2 cut(s)	598, 745
FbaI	TGATCA	1 cut(s)	1974
Fnu4HI	GCNGC	6 cut(s)	75, 342, 631, 1859, 2312, 2467
FokI	GGATG	8 cut(s)	739, 1112, 1196, 1394, 1519, 2120, 2456, 2508
FriOI	GRGCYC	4 cut(s)	441, 546, 1654, 2121
Fsp4HI	GCNGC	6 cut(s)	75, 342, 631, 1859, 2312, 2467
FspAI	RTGCGCAY	1 cut(s)	675
FspBI	CTAG	5 cut(s)	840, 1574, 1748, 2270, 2331
FspI	TGCGCA	1 cut(s)	675
GlaI	GCGC	2 cut(s)	345, 675
GluI	GCNGC	6 cut(s)	75, 342, 631, 1859, 2312, 2467
GsaI	CCCAGC	3 cut(s)	208, 491, 1029
GsuI	CTGGAG	2 cut(s)	260, 611
HaeII	RGCGCY	1 cut(s)	347
HaeIII	GGCC	4 cut(s)	816, 1343, 2538, 2563
HapII	CCGG	4 cut(s)	505, 1705, 2171, 2340
HgaI	GACGC	4 cut(s)	292, 307, 961, 1216
HhaI	GCGC	2 cut(s)	346, 676
Hin1I	GRCGYC	2 cut(s)	318, 972
Hin6I	GCGC	2 cut(s)	344, 674
HinP1I	GCGC	2 cut(s)	344, 674
HincII	GTYRAC	1 cut(s)	1915
HindII	GTYRAC	1 cut(s)	1915
HinfI	GANTC	8 cut(s)	64, 553, 728, 964, 1076, 2034, 2186, 2198
HpaII	CCGG	4 cut(s)	505, 1705, 2171, 2340
HphI	GGTGA	3 cut(s)	215, 2089, 2353
Hpy166II	GTNNAC	5 cut(s)	336, 916, 949, 1915, 2038
Hpy188III	TCNNGA	4 cut(s)	574, 907, 1455, 2195
Hpy8I	GTNNAC	5 cut(s)	336, 916, 949, 1915, 2038
Hpy99I	CGWCG	2 cut(s)	308, 1232
HpyCH4III	ACNGT	4 cut(s)	498, 902, 1667, 2307
HpyCH4IV	ACGT	2 cut(s)	1109, 1322
HpyF10VI	GCNNNNNNNGC	6 cut(s)	245, 457, 740, 749, 986, 2308
HpyF3I	CTNAG	9 cut(s)	20, 347, 422, 767, 1453, 1646, 1906, 1993, 2447
HpySE526I	ACGT	2 cut(s)	1109, 1322
Hsp92I	GRCGYC	2 cut(s)	318, 972
HspAI	GCGC	2 cut(s)	344, 674
KflI	GGGWCCC	1 cut(s)	208
KroI	GCCGGC	1 cut(s)	504
KroNI	GCCGGC	1 cut(s)	506
Ksp22I	TGATCA	1 cut(s)	1974
Kzo9I	GATC	8 cut(s)	326, 576, 1738, 1974, 2191, 2294, 2442, 2622
LguI	GCTCTTC	1 cut(s)	1271
LmnI	GCTCC	4 cut(s)	279, 592, 1570, 2400
Lsp1109I	GCAGC	4 cut(s)	617, 1845, 2298, 2478
LweI	GCATC	5 cut(s)	761, 1158, 2074, 2478, 2508
MaeI	CTAG	5 cut(s)	840, 1574, 1748, 2270, 2331
MaeII	ACGT	2 cut(s)	1109, 1322
MaeIII	GTNAC	5 cut(s)	874, 1627, 1724, 2284, 2553
MalI	GATC	8 cut(s)	328, 578, 1740, 1976, 2193, 2296, 2444, 2624
MboI	GATC	8 cut(s)	326, 576, 1738, 1974, 2191, 2294, 2442, 2622
MfeI	CAATTG	2 cut(s)	1154, 2453
MhlI	GDGCHC	5 cut(s)	441, 546, 1654, 2121, 2329
MlsI	TGGCCA	2 cut(s)	2538, 2563
MluNI	TGGCCA	2 cut(s)	2538, 2563
MlyI	GAGTC	3 cut(s)	737, 2043, 2207
MmeI	TCCRAC	2 cut(s)	705, 1077
Mox20I	TGGCCA	2 cut(s)	2538, 2563
Mph1103I	ATGCAT	1 cut(s)	2501
MroNI	GCCGGC	1 cut(s)	504
MscI	TGGCCA	2 cut(s)	2538, 2563
MseI	TTAA	7 cut(s)	1007, 1206, 1542, 2228, 2370, 2571, 2640
MslI	CAYNNNNRTG	1 cut(s)	911
Msp20I	TGGCCA	2 cut(s)	2538, 2563
MspA1I	CMGCKG	1 cut(s)	204
MspI	CCGG	4 cut(s)	505, 1705, 2171, 2340
MspR9I	CCNGG	8 cut(s)	278, 1694, 1706, 1848, 2290, 2411, 2535, 2560
MunI	CAATTG	2 cut(s)	1154, 2453
Mva1269I	GAATGC	2 cut(s)	1723, 1999
MvaI	CCWGG	7 cut(s)	278, 1694, 1848, 2290, 2411, 2535, 2560
MvnI	CGCG	1 cut(s)	264
MwoI	GCNNNNNNNGC	6 cut(s)	245, 457, 740, 749, 986, 2308
NaeI	GCCGGC	1 cut(s)	506
NciI	CCSGG	1 cut(s)	1706
NdeI	CATATG	2 cut(s)	598, 745
NdeII	GATC	8 cut(s)	326, 576, 1738, 1974, 2191, 2294, 2442, 2622
NgoMIV	GCCGGC	1 cut(s)	504
NheI	GCTAGC	1 cut(s)	1573
NlaIV	GGNNCC	6 cut(s)	209, 210, 275, 447, 594, 2396
NmuCI	GTSAC	2 cut(s)	874, 1627
NsbI	TGCGCA	1 cut(s)	675
NsiI	ATGCAT	1 cut(s)	2501
NspI	RCATGY	5 cut(s)	674, 993, 1223, 1714, 2020
PagI	TCATGA	1 cut(s)	906
PaqCI	CACCTGC	1 cut(s)	1490
PceI	AGGCCT	1 cut(s)	1343
PciSI	GCTCTTC	1 cut(s)	1271
PcsI	WCGNNNNNNNCGW	2 cut(s)	303, 2631
PctI	GAATGC	2 cut(s)	1723, 1999
PdiI	GCCGGC	1 cut(s)	506
PfeI	GAWTC	5 cut(s)	64, 553, 964, 1076, 2186
PflFI	GACNNNGTC	1 cut(s)	498
PfoI	TCCNGGA	1 cut(s)	276
PinAI	ACCGGT	1 cut(s)	2339
PkrI	GCNGC	6 cut(s)	76, 343, 632, 1860, 2313, 2468
Ple19I	CGATCG	1 cut(s)	2625
PleI	GAGTC	3 cut(s)	736, 2042, 2206
PpsI	GAGTC	3 cut(s)	736, 2042, 2206
Ppu21I	YACGTR	1 cut(s)	1110
PpuMI	RGGWCCY	2 cut(s)	208, 2138
PshBI	ATTAAT	1 cut(s)	2571
PsiI	TTATAA	2 cut(s)	698, 996
Psp124BI	GAGCTC	2 cut(s)	441, 546
Psp5II	RGGWCCY	2 cut(s)	208, 2138
Psp6I	CCWGG	7 cut(s)	276, 1692, 1846, 2288, 2409, 2533, 2558
PspEI	GGTNACC	2 cut(s)	2284, 2553
PspFI	CCCAGC	3 cut(s)	204, 487, 1025
PspGI	CCWGG	7 cut(s)	276, 1692, 1846, 2288, 2409, 2533, 2558
PspN4I	GGNNCC	6 cut(s)	209, 210, 275, 447, 594, 2396
PspPI	GGNCC	3 cut(s)	208, 2138, 2220
PspPPI	RGGWCCY	2 cut(s)	208, 2138
PsrI	GAACNNNNNNTAC	4 cut(s)	1651, 1683, 2335, 2367
PstI	CTGCAG	1 cut(s)	2583
PstNI	CAGNNNCTG	1 cut(s)	2311
PsyI	GACNNNGTC	1 cut(s)	498
PvuI	CGATCG	1 cut(s)	2625
RsaI	GTAC	8 cut(s)	473, 845, 1065, 1244, 1325, 1351, 1894, 2338
RsaNI	GTAC	8 cut(s)	472, 844, 1064, 1243, 1324, 1350, 1893, 2337
RseI	CAYNNNNRTG	1 cut(s)	911
SacI	GAGCTC	2 cut(s)	441, 546
SapI	GCTCTTC	1 cut(s)	1271
SaqAI	TTAA	7 cut(s)	1007, 1206, 1542, 2228, 2370, 2571, 2640
SatI	GCNGC	6 cut(s)	75, 342, 631, 1859, 2312, 2467
Sau3AI	GATC	8 cut(s)	326, 576, 1738, 1974, 2191, 2294, 2442, 2622
Sau96I	GGNCC	3 cut(s)	208, 2138, 2220
SchI	GAGTC	3 cut(s)	737, 2043, 2207
ScrFI	CCNGG	8 cut(s)	278, 1694, 1706, 1848, 2290, 2411, 2535, 2560
SduI	GDGCHC	5 cut(s)	441, 546, 1654, 2121, 2329
SfaNI	GCATC	5 cut(s)	761, 1158, 2074, 2478, 2508
SfcI	CTRYAG	3 cut(s)	1987, 2303, 2579
SinI	GGWCC	3 cut(s)	208, 2138, 2220
SmiMI	CAYNNNNRTG	1 cut(s)	911
SmlI	CTYRAG	1 cut(s)	2120
SmoI	CTYRAG	1 cut(s)	2120
SseBI	AGGCCT	1 cut(s)	1343
SspI	AATATT	1 cut(s)	886
SspMI	CTAG	5 cut(s)	840, 1574, 1748, 2270, 2331
SstI	GAGCTC	2 cut(s)	441, 546
StuI	AGGCCT	1 cut(s)	1343
StyD4I	CCNGG	8 cut(s)	276, 1692, 1704, 1846, 2288, 2409, 2533, 2558
StyI	CCWWGG	1 cut(s)	756
TaaI	ACNGT	4 cut(s)	498, 902, 1667, 2307
TaiI	ACGT	2 cut(s)	1112, 1325
TaqI	TCGA	4 cut(s)	297, 1230, 1427, 2621
TaqII	GACCGA	1 cut(s)	2237
TatI	WGTACW	1 cut(s)	1063
TauI	GCSGC	2 cut(s)	77, 344
TfiI	GAWTC	5 cut(s)	64, 553, 964, 1076, 2186
Tru1I	TTAA	7 cut(s)	1007, 1206, 1542, 2228, 2370, 2571, 2640
Tru9I	TTAA	7 cut(s)	1007, 1206, 1542, 2228, 2370, 2571, 2640
TscAI	CASTG	3 cut(s)	503, 1205, 2312
TseFI	GTSAC	2 cut(s)	874, 1627
TseI	GCWGC	4 cut(s)	630, 1858, 2311, 2466
Tsp45I	GTSAC	2 cut(s)	874, 1627
TspRI	CASTG	3 cut(s)	503, 1205, 2312
Tth111I	GACNNNGTC	1 cut(s)	498
VpaK11BI	GGWCC	3 cut(s)	208, 2138, 2220
VspI	ATTAAT	1 cut(s)	2571
XapI	RAATTY	6 cut(s)	704, 1564, 1621, 2068, 2425, 2636
XceI	RCATGY	5 cut(s)	674, 993, 1223, 1714, 2020
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2045
XspI	CTAG	5 cut(s)	840, 1574, 1748, 2270, 2331
Zsp2I	ATGCAT	1 cut(s)	2501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.