pycom12g12870

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009024
Physical Location & Seq
Forward (+)
15328826 .. 15331463
2638 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1149 bp
ATGGGCAACTCCCACGGCCACCACCGTAAACGCCGTAACGGCCCCGGGCCCAGTGAAACGGCCAATTCGGGCTTTAAATATGGTTCGCTGGATCAAGCAGCTCAAATCCCTAGCTCTAGCTCAACGTCAGCTAAGAAGACCAGCAGCAGCATGGTGGTTGCCTTGCCGTACGCTCACGTCGACTCGAGCTTGCGCGCCCTCTCCGCCCAGGCCGAGGGCTTCGGCCGGCTGGCAATCGGCGGCCTCCACGGCGCCGTTTACCACGTCACCACTCTAGCAGATGATGGTCCGGGTTCACTTAGGGAAGGATGTCGTCAGAAAGAACCGCTTTGGATTGTCTTCGAAATTTCAGGCACTATCCATCTCCAATCTTACTTGAACGTGTCATCGTATAAGACCATAGATGGGCGCGGTCAGAGGATTAAACTTACAGGGAAAGGGTTGAGGTTGAAGGAATGTGAACATGTCATTGTATGCAATTTAGAGTTTGAAGGTGGTAGAGGGCCAGATGTTGATGGCATTCAAATAAAACCCAATTCTAAACACATATGGATAGATCGCTGCAGCCTTCGTGATTATGAAGACGGACTTATCGATATAACCAGAGGAAGCACTGATATTACAATTTCCAGGTGTCATTTTTCACAGCATGATAAGACAATGCTGATTGGCGCAGATCCCTCTCATATTGATGACAGATGCATCCGGGTTACCATTCACCACTGTTTCTTTGATGGAACCAGACAGAGGCATCCTCGTGTTAGATTTGCGAAAGTACATCTATATAACAACTACACCCGAAACTGGGGCATATATGCTGTTTGTGCTAGTGTAGAATCGCAGATATTCTCGGAATGCAATATATATGAAGCAGGACAGAAAAAAGTGGCATTTAAGTACCTTACGGAGAAGGCAGCGGACAAGGAGACGGCAATGACTGGCCACATTAAGTCCGAAGGCGACTTATTTGTAACGGGAACTCAAGCAGGATTATCCCCCGATGGTGGTGAACATTGCGTGTTTCATCCTACCCAGCACTATCAGACATGGACAAAAGGACCGCCCATGGACGATCTTAAGCATGTTCTCCAACATTGCACAGGATGGCAGTGTGTTCCGCGGCCAGCTGATCAAGGATTAGCGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

383

Amino Acids

42.24

Weight (kDa)

8.52

Isoelectric Point (pI)

31.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 124 - 324 2.4e-29 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 251
AccI GTMKAC 1 cut(s) 180
AccII CGCG 3 cut(s) 195, 411, 1120
AciI CCGC 8 cut(s) 204, 240, 326, 411, 917, 1061, 1118, 1120
AclWI GGATC 2 cut(s) 99, 671
AcoI YGGCCR 5 cut(s) 16, 60, 223, 940, 1121
AcsI RAATTY 1 cut(s) 345
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 3 cut(s) 170, 777, 899
AfiI CCNNNNNNNGG 6 cut(s) 214, 405, 747, 804, 805, 1004
AflII CTTAAG 1 cut(s) 1076
AflIII ACRYGT 2 cut(s) 381, 463
AgsI TTSAA 4 cut(s) 379, 451, 491, 524
AjiI CACGTC 2 cut(s) 178, 265
AjnI CCWGG 2 cut(s) 207, 629
AluBI AGCT 6 cut(s) 101, 114, 120, 131, 189, 1127
AluI AGCT 6 cut(s) 101, 114, 120, 131, 189, 1127
Alw26I GTCTC 1 cut(s) 920
AlwI GGATC 2 cut(s) 99, 671
Ama87I CYCGRG 2 cut(s) 44, 184
ApaI GGGCCC 1 cut(s) 51
ApeKI GCWGC 6 cut(s) 98, 144, 147, 561, 564, 914
ApoI RAATTY 1 cut(s) 345
AspLEI GCGC 5 cut(s) 195, 197, 254, 411, 674
AspS9I GGNCC 6 cut(s) 41, 47, 48, 287, 503, 1058
AsuC2I CCSGG 4 cut(s) 45, 46, 291, 707
AsuHPI GGTGA 3 cut(s) 259, 710, 1019
AsuII TTCGAA 1 cut(s) 342
AvaI CYCGRG 2 cut(s) 44, 184
AvaII GGWCC 2 cut(s) 287, 1058
BaeGI GKGCMC 1 cut(s) 51
BalI TGGCCA 1 cut(s) 942
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 1 cut(s) 51
BauI CACGAG 1 cut(s) 756
BbsI GAAGAC 3 cut(s) 143, 331, 588
BbvI GCAGC 6 cut(s) 110, 156, 159, 548, 576, 926
BccI CCATC 7 cut(s) 278, 369, 398, 509, 728, 995, 1098
BceAI ACGGC 8 cut(s) 18, 31, 55, 75, 151, 239, 265, 945
BciT130I CCWGG 2 cut(s) 209, 631
BclI TGATCA 1 cut(s) 1129
BcnI CCSGG 4 cut(s) 45, 46, 291, 707
BcoDI GTCTC 1 cut(s) 920
BfaI CTAG 4 cut(s) 111, 117, 275, 828
BfmI CTRYAG 1 cut(s) 562
BfoI RGCGCY 1 cut(s) 255
BfrI CTTAAG 1 cut(s) 1076
BglI GCCNNNNNGGC 2 cut(s) 39, 249
BisI GCNGC 8 cut(s) 99, 145, 148, 241, 562, 565, 915, 1121
BlsI GCNGC 8 cut(s) 100, 146, 149, 242, 563, 566, 916, 1122
Bme1390I CCNGG 6 cut(s) 45, 46, 209, 291, 631, 707
Bme18I GGWCC 2 cut(s) 287, 1058
BmeT110I CYCGRG 2 cut(s) 44, 184
BmgBI CACGTC 2 cut(s) 178, 265
BmgT120I GGNCC 6 cut(s) 41, 47, 48, 287, 503, 1058
BmiI GGNNCC 4 cut(s) 43, 49, 253, 739
BmrFI CCNGG 6 cut(s) 45, 46, 209, 291, 631, 707
BmrI ACTGGG 2 cut(s) 45, 814
BmsI GCATC 3 cut(s) 689, 711, 760
BmuI ACTGGG 2 cut(s) 45, 814
BpiI GAAGAC 3 cut(s) 143, 331, 588
BplI GAGNNNNNCTC 2 cut(s) 739, 771
Bpu14I TTCGAA 1 cut(s) 342
BpuEI CTTGAG 1 cut(s) 966
BpuMI CCSGG 4 cut(s) 45, 46, 291, 707
Bsa29I ATCGAT 1 cut(s) 594
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 8 cut(s) 13, 43, 44, 207, 213, 247, 1065, 1118
Bsc4I CCNNNNNNNGG 6 cut(s) 214, 405, 747, 804, 805, 1004
Bse118I RCCGGY 1 cut(s) 225
Bse1I ACTGG 3 cut(s) 51, 809, 943
Bse3DI GCAATG 3 cut(s) 939, 1012, 1093
BseBI CCWGG 2 cut(s) 209, 631
BseCI ATCGAT 1 cut(s) 594
BseDI CCNNGG 8 cut(s) 13, 43, 44, 207, 213, 247, 1065, 1118
BseGI GGATG 5 cut(s) 314, 702, 751, 1024, 1109
BseLI CCNNNNNNNGG 6 cut(s) 214, 405, 747, 804, 805, 1004
BseMI GCAATG 3 cut(s) 939, 1012, 1093
BseNI ACTGG 3 cut(s) 51, 809, 943
BsePI GCGCGC 1 cut(s) 193
BseSI GKGCMC 1 cut(s) 51
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 6 cut(s) 110, 156, 159, 548, 576, 926
BseYI CCCAGC 1 cut(s) 1032
Bsh1236I CGCG 3 cut(s) 195, 411, 1120
Bsh1285I CGRYCG 1 cut(s) 226
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 594
BsiEI CGRYCG 1 cut(s) 226
BsiHKCI CYCGRG 2 cut(s) 44, 184
BsiSI CCGG 4 cut(s) 45, 226, 290, 706
BsiWI CGTACG 1 cut(s) 168
BslI CCNNNNNNNGG 6 cut(s) 214, 405, 747, 804, 805, 1004
BsmAI GTCTC 1 cut(s) 920
BsmBI CGTCTC 1 cut(s) 920
BsmI GAATGC 2 cut(s) 519, 860
BsoBI CYCGRG 2 cut(s) 44, 184
Bsp119I TTCGAA 1 cut(s) 342
Bsp120I GGGCCC 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 5 cut(s) 91, 556, 676, 1072, 1129
Bsp19I CCATGG 1 cut(s) 1065
BspACI CCGC 8 cut(s) 204, 240, 326, 411, 917, 1061, 1118, 1120
BspDI ATCGAT 1 cut(s) 594
BspFNI CGCG 3 cut(s) 195, 411, 1120
BspLI GGNNCC 4 cut(s) 43, 49, 253, 739
BspMAI CTGCAG 1 cut(s) 566
BspPI GGATC 2 cut(s) 99, 671
BspT104I TTCGAA 1 cut(s) 342
BspT107I GGYRCC 1 cut(s) 251
BspTI CTTAAG 1 cut(s) 1076
BsrDI GCAATG 3 cut(s) 939, 1012, 1093
BsrFI RCCGGY 1 cut(s) 225
BsrI ACTGG 3 cut(s) 51, 809, 943
BssAI RCCGGY 1 cut(s) 225
BssECI CCNNGG 8 cut(s) 13, 43, 44, 207, 213, 247, 1065, 1118
BssHII GCGCGC 1 cut(s) 193
BssMI GATC 5 cut(s) 91, 556, 676, 1072, 1129
BssNI GRCGYC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 756
BssT1I CCWWGG 1 cut(s) 1065
Bst2BI CACGAG 1 cut(s) 756
Bst2UI CCWGG 2 cut(s) 209, 631
Bst4CI ACNGT 2 cut(s) 26, 725
BstACI GRCGYC 1 cut(s) 252
BstAFI CTTAAG 1 cut(s) 1076
BstBI TTCGAA 1 cut(s) 342
BstC8I GCNNGC 5 cut(s) 191, 195, 227, 231, 1125
BstDEI CTNAG 2 cut(s) 132, 299
BstDSI CCRYGG 4 cut(s) 13, 247, 1065, 1118
BstEII GGTNACC 1 cut(s) 709
BstF5I GGATG 5 cut(s) 314, 702, 751, 1024, 1109
BstFNI CGCG 3 cut(s) 195, 411, 1120
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 5 cut(s) 195, 197, 254, 411, 674
BstKTI GATC 5 cut(s) 94, 559, 679, 1075, 1132
BstMAI GTCTC 1 cut(s) 920
BstMBI GATC 5 cut(s) 91, 556, 676, 1072, 1129
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 4 cut(s) 39, 203, 249, 824
BstNI CCWGG 2 cut(s) 209, 631
BstNSI RCATGY 2 cut(s) 467, 1085
BstPI GGTNACC 1 cut(s) 709
BstSCI CCNGG 6 cut(s) 43, 44, 207, 289, 629, 705
BstSFI CTRYAG 1 cut(s) 562
BstSLI GKGCMC 1 cut(s) 51
BstUI CGCG 3 cut(s) 195, 411, 1120
BstV1I GCAGC 6 cut(s) 110, 156, 159, 548, 576, 926
BstV2I GAAGAC 3 cut(s) 143, 331, 588
BstX2I RGATCY 1 cut(s) 676
BstYI RGATCY 1 cut(s) 676
BstZI CGGCCG 1 cut(s) 223
Bsu15I ATCGAT 1 cut(s) 594
BsuTUI ATCGAT 1 cut(s) 594
BtgI CCRYGG 4 cut(s) 13, 247, 1065, 1118
BtrI CACGTC 2 cut(s) 178, 265
BtsCI GGATG 5 cut(s) 314, 702, 751, 1024, 1109
BtsI GCAGTG 1 cut(s) 1115
BtsIMutI CAGTG 4 cut(s) 58, 612, 721, 1115
Cac8I GCNNGC 5 cut(s) 191, 195, 227, 231, 1125
CfoI GCGC 5 cut(s) 195, 197, 254, 411, 674
Cfr10I RCCGGY 1 cut(s) 225
Cfr13I GGNCC 6 cut(s) 41, 47, 48, 287, 503, 1058
Cfr42I CCGCGG 1 cut(s) 1121
Cfr9I CCCGGG 1 cut(s) 44
ClaI ATCGAT 1 cut(s) 594
Csp6I GTAC 3 cut(s) 169, 776, 898
CviAII CATG 6 cut(s) 151, 464, 650, 1047, 1066, 1082
CviQI GTAC 3 cut(s) 169, 776, 898
DdeI CTNAG 2 cut(s) 132, 299
DinI GGCGCC 1 cut(s) 253
DpnI GATC 5 cut(s) 93, 558, 678, 1074, 1131
DpnII GATC 5 cut(s) 91, 556, 676, 1072, 1129
DraI TTTAAA 1 cut(s) 76
EaeI YGGCCR 5 cut(s) 16, 60, 223, 940, 1121
EagI CGGCCG 1 cut(s) 223
EciI GGCGGA 1 cut(s) 193
EclXI CGGCCG 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 1065
Eco24I GRGCYC 1 cut(s) 51
Eco47I GGWCC 2 cut(s) 287, 1058
Eco52I CGGCCG 1 cut(s) 223
Eco88I CYCGRG 2 cut(s) 44, 184
Eco91I GGTNACC 1 cut(s) 709
EcoO65I GGTNACC 1 cut(s) 709
EcoRII CCWGG 2 cut(s) 207, 629
EcoT14I CCWWGG 1 cut(s) 1065
EcoT22I ATGCAT 1 cut(s) 704
EcoT38I GRGCYC 1 cut(s) 51
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 1065
Esp3I CGTCTC 1 cut(s) 920
FaeI CATG 6 cut(s) 154, 467, 653, 1050, 1069, 1085
FalI AAGNNNNNCTT 4 cut(s) 312, 344, 573, 605
FatI CATG 6 cut(s) 150, 463, 649, 1046, 1065, 1081
FauNDI CATATG 1 cut(s) 548
FbaI TGATCA 1 cut(s) 1129
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 8 cut(s) 99, 145, 148, 241, 562, 565, 915, 1121
FokI GGATG 5 cut(s) 321, 689, 738, 1011, 1116
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 8 cut(s) 99, 145, 148, 241, 562, 565, 915, 1121
FspBI CTAG 4 cut(s) 111, 117, 275, 828
GlaI GCGC 5 cut(s) 194, 196, 253, 410, 673
GluI GCNGC 8 cut(s) 99, 145, 148, 241, 562, 565, 915, 1121
GsaI CCCAGC 1 cut(s) 1036
HaeII RGCGCY 1 cut(s) 255
HapII CCGG 4 cut(s) 45, 226, 290, 706
HhaI GCGC 5 cut(s) 195, 197, 254, 411, 674
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 6 cut(s) 154, 467, 653, 1050, 1069, 1085
Hin6I GCGC 5 cut(s) 193, 195, 252, 409, 672
HinP1I GCGC 5 cut(s) 193, 195, 252, 409, 672
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 2 cut(s) 182, 836
HpaII CCGG 4 cut(s) 45, 226, 290, 706
HphI GGTGA 3 cut(s) 259, 710, 1019
Hpy166II GTNNAC 6 cut(s) 29, 181, 259, 296, 461, 1010
Hpy188I TCNGA 5 cut(s) 318, 417, 853, 955, 1044
Hpy188III TCNNGA 1 cut(s) 572
Hpy8I GTNNAC 6 cut(s) 29, 181, 259, 296, 461, 1010
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 6 cut(s) 299, 445, 485, 578, 904, 950
HpyCH4III ACNGT 2 cut(s) 26, 725
HpyCH4IV ACGT 4 cut(s) 125, 177, 264, 381
HpyCH4V TGCA 5 cut(s) 477, 564, 702, 858, 1098
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 203, 249, 824
HpyF3I CTNAG 2 cut(s) 132, 299
HpySE526I ACGT 4 cut(s) 125, 177, 264, 381
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 6 cut(s) 154, 467, 653, 1050, 1069, 1085
HspAI GCGC 5 cut(s) 193, 195, 252, 409, 672
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 225
KroNI GCCGGC 1 cut(s) 227
Ksp22I TGATCA 1 cut(s) 1129
KspI CCGCGG 1 cut(s) 1121
Kzo9I GATC 5 cut(s) 91, 556, 676, 1072, 1129
Lsp1109I GCAGC 6 cut(s) 110, 156, 159, 548, 576, 926
LweI GCATC 3 cut(s) 689, 711, 760
MaeI CTAG 4 cut(s) 111, 117, 275, 828
MaeII ACGT 4 cut(s) 125, 177, 264, 381
MaeIII GTNAC 4 cut(s) 35, 265, 709, 970
MalI GATC 5 cut(s) 93, 558, 678, 1074, 1131
MboI GATC 5 cut(s) 91, 556, 676, 1072, 1129
MboII GAAGA 3 cut(s) 148, 331, 593
MflI RGATCY 1 cut(s) 676
MhlI GDGCHC 1 cut(s) 51
MlsI TGGCCA 1 cut(s) 942
MluCI AATT 5 cut(s) 64, 345, 478, 535, 624
MluNI TGGCCA 1 cut(s) 942
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 1114
Mox20I TGGCCA 1 cut(s) 942
Mph1103I ATGCAT 1 cut(s) 704
MroNI GCCGGC 1 cut(s) 225
MscI TGGCCA 1 cut(s) 942
MseI TTAA 5 cut(s) 75, 423, 894, 948, 1077
MslI CAYNNNNRTG 2 cut(s) 690, 756
Msp20I TGGCCA 1 cut(s) 942
MspA1I CMGCKG 3 cut(s) 917, 1120, 1127
MspCI CTTAAG 1 cut(s) 1076
MspI CCGG 4 cut(s) 45, 226, 290, 706
MspR9I CCNGG 6 cut(s) 45, 46, 209, 291, 631, 707
Mva1269I GAATGC 2 cut(s) 519, 860
MvaI CCWGG 2 cut(s) 209, 631
MvnI CGCG 3 cut(s) 195, 411, 1120
MwoI GCNNNNNNNGC 4 cut(s) 39, 203, 249, 824
NaeI GCCGGC 1 cut(s) 227
NarI GGCGCC 1 cut(s) 252
NciI CCSGG 4 cut(s) 45, 46, 291, 707
NcoI CCATGG 1 cut(s) 1065
NdeI CATATG 1 cut(s) 548
NdeII GATC 5 cut(s) 91, 556, 676, 1072, 1129
NgoMIV GCCGGC 1 cut(s) 225
NlaIII CATG 6 cut(s) 154, 467, 653, 1050, 1069, 1085
NlaIV GGNNCC 4 cut(s) 43, 49, 253, 739
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 265
NsiI ATGCAT 1 cut(s) 704
NspI RCATGY 2 cut(s) 467, 1085
NspV TTCGAA 1 cut(s) 342
PaeR7I CTCGAG 1 cut(s) 184
PauI GCGCGC 1 cut(s) 193
PciI ACATGT 1 cut(s) 463
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 591
PctI GAATGC 2 cut(s) 519, 860
PdiI GCCGGC 1 cut(s) 227
PfeI GAWTC 1 cut(s) 836
Pfl23II CGTACG 1 cut(s) 168
PkrI GCNGC 8 cut(s) 100, 146, 149, 242, 563, 566, 916, 1122
PleI GAGTC 1 cut(s) 176
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 176
PscI ACATGT 1 cut(s) 463
Psp6I CCWGG 2 cut(s) 207, 629
PspEI GGTNACC 1 cut(s) 709
PspFI CCCAGC 1 cut(s) 1032
PspGI CCWGG 2 cut(s) 207, 629
PspLI CGTACG 1 cut(s) 168
PspN4I GGNNCC 4 cut(s) 43, 49, 253, 739
PspOMI GGGCCC 1 cut(s) 47
PspPI GGNCC 6 cut(s) 41, 47, 48, 287, 503, 1058
PspXI VCTCGAGB 1 cut(s) 184
PstI CTGCAG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 676
PteI GCGCGC 1 cut(s) 193
PvuII CAGCTG 1 cut(s) 1127
RsaI GTAC 3 cut(s) 170, 777, 899
RsaNI GTAC 3 cut(s) 169, 776, 898
RseI CAYNNNNRTG 2 cut(s) 690, 756
SacII CCGCGG 1 cut(s) 1121
SalI GTCGAC 1 cut(s) 179
SaqAI TTAA 5 cut(s) 75, 423, 894, 948, 1077
SatI GCNGC 8 cut(s) 99, 145, 148, 241, 562, 565, 915, 1121
Sau3AI GATC 5 cut(s) 91, 556, 676, 1072, 1129
Sau96I GGNCC 6 cut(s) 41, 47, 48, 287, 503, 1058
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 6 cut(s) 45, 46, 209, 291, 631, 707
SduI GDGCHC 1 cut(s) 51
SfaNI GCATC 3 cut(s) 689, 711, 760
SfcI CTRYAG 1 cut(s) 562
SfoI GGCGCC 1 cut(s) 253
Sfr274I CTCGAG 1 cut(s) 184
Sfr303I CCGCGG 1 cut(s) 1121
SfuI TTCGAA 1 cut(s) 342
SgrBI CCGCGG 1 cut(s) 1121
SinI GGWCC 2 cut(s) 287, 1058
SlaI CTCGAG 1 cut(s) 184
SmaI CCCGGG 1 cut(s) 46
SmiMI CAYNNNNRTG 2 cut(s) 690, 756
SmlI CTYRAG 3 cut(s) 184, 981, 1076
SmoI CTYRAG 3 cut(s) 184, 981, 1076
Sse9I AATT 5 cut(s) 64, 345, 478, 535, 624
SsiI CCGC 8 cut(s) 204, 240, 326, 411, 917, 1061, 1118, 1120
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 4 cut(s) 111, 117, 275, 828
StyD4I CCNGG 6 cut(s) 43, 44, 207, 289, 629, 705
StyI CCWWGG 1 cut(s) 1065
TaaI ACNGT 2 cut(s) 26, 725
TaiI ACGT 4 cut(s) 128, 180, 267, 384
TaqI TCGA 4 cut(s) 180, 185, 342, 594
TasI AATT 5 cut(s) 64, 345, 478, 535, 624
TatI WGTACW 1 cut(s) 775
TauI GCSGC 2 cut(s) 243, 1123
TfiI GAWTC 1 cut(s) 836
Tru1I TTAA 5 cut(s) 75, 423, 894, 948, 1077
Tru9I TTAA 5 cut(s) 75, 423, 894, 948, 1077
TscAI CASTG 4 cut(s) 58, 619, 728, 1115
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 6 cut(s) 98, 144, 147, 561, 564, 914
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 3 cut(s) 594, 882, 1013
TspGWI ACGGA 2 cut(s) 600, 920
TspMI CCCGGG 1 cut(s) 44
TspRI CASTG 4 cut(s) 58, 619, 728, 1115
Vha464I CTTAAG 1 cut(s) 1076
VpaK11BI GGWCC 2 cut(s) 287, 1058
XapI RAATTY 1 cut(s) 345
XceI RCATGY 2 cut(s) 467, 1085
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XhoI CTCGAG 1 cut(s) 184
XmaI CCCGGG 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 4 cut(s) 111, 117, 275, 828
Zsp2I ATGCAT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.