pycom12g13160

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009698
Physical Location & Seq
Forward (+)
15654205 .. 15656441
2237 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1611 bp
ATGTCTTTTCAGAATCACGGCCTTTGGATGGCAAAGGGTACCGGGTGTTTAAACGAAGGTGAGGCACTGTATGATAATTCTTCTAGAATTGAGCCTAAGCGTTCTCATCAGTGGTTCATGGATGGTCCTGAGGTAGGGATATCCCCCAACAAGAAACAGGTTGTTGAAGTTCCAAACAACAATCTGTATTCGGGAATGTTAAATGCAAATGTTTCTCCGTGGGGAAATGTTCCTGGTTTTCACTCATTCTCTGGCCAATTCGCTGAAAGGTTGTTTGATTGTGAAATAGACCGGGCTGTGAAATTTGATGACACGAACATTCCGTCTGTTGGAACGGAAAAGATGAATTTGGCTAGAAAAGTTAATGAGGATCTATTTGTGAATGACTCCTCATTTGGTCTATCTATGTCCAATACACTAGAAGATCCTAGATCCAGTCCTAATTACGGTGGATTTAGAAAAGTCAAAGTTAGTGAGGTAAAGGACTCTGAGAATGGCATGCCCTTTTCAATAGGACAAACGTATATTCAGGCAGATAATGATGCCATGTTGGCGTCTCAATCTTACAAGGCCAAAGACAACTCAGCTTCCACGGGTCCCATTTATAAGAAAGGGGATGACAACTTCATATCCATGAGTGATACCTACAGCAGGGCAGATAACAGCTTCATATCAATGGGGCAACCCTTTAACAAAGGGGATGAAAGCATATCGTTTGGTCAATCATACAAGGAGAGCAATAACACCCTGTTAATGGGTCAGACATGCAATAAGAGTGACAATAATATCATTTCTATCGGCCAAACCTATAAGGTAGAGGAAAATACCATATCAACAGATAATTTCTACAATAAAGGGGAAGAGAGTACCATATCCGTTGGTCATGCGACTCATGCCTACAGCAAGGGTGACAACAATATGTTGTCAGTTGGGCACTCTTATAATAAGCGAGAGGGTACTATCATATCCTTTGGCGGATGTGATGATGATGATGTGAATACCTCTGCAGTCTTTGGTTATGAACTTCTGATGGGTCAACCTTTTGTGAAAACAGAAACCGTGAATGAGAAAGAGTTGGGCAAATCAAATTCCGGTGCATTTGTTAATGTACCTCATATAACTGCTGGAAACGAAAATGTCCTGAAAAAGATAGTTGAACAGAAAACGTCTAAGAAGGTTCCTCCGAACAACTTCCCTTCAAATGTGCGAAGTTTGCTGTCAACAGGTATGCTGGATGGAGTTCCTGTAAAGTATACAGCCTGGTCGCGGGAGAAGGAGCTGCAGGGTGTTATCAAAGGTTGTGGGTATCTATGTGGCTGTCAGTCGTGCAACTTCTCAAAGGTAATCAATGCATATGAATTTGAGCGTCATGCTGGTTGCAAAACGAAACACCCAAATAATCACATACATTTTGAGAATGGTAAAACTATATATGGGACCGTTCAAGAGCTCAGGAGCACACCACAGAATATGCTGTTTGAAGTTATTCAGACTATTACTGGTTCGCCTATCAATCAGAAATCCTTCCGCCTTTGGAAAGAATCCTTTCTGGCAGCAACACGTGAACTTCAGCGTATATATGGCAAGGAAGAGGGGAAACAACTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas

Protein Analysis

537

Amino Acids

59.46

Weight (kDa)

6.17

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 429 - 483 5.4e-14 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 606, 942
Acc65I GGTACC 1 cut(s) 38
AccB1I GGYRCC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 1253
AccII CGCG 1 cut(s) 1267
AciI CCGC 3 cut(s) 975, 1267, 1528
AclWI GGATC 3 cut(s) 378, 419, 426
AcoI YGGCCR 2 cut(s) 253, 799
AcsI RAATTY 4 cut(s) 302, 346, 1087, 1358
AcuI CTGAAG 1 cut(s) 1553
AcvI CACGTG 1 cut(s) 1562
AcyI GRCGYC 1 cut(s) 554
AfaI GTAC 4 cut(s) 40, 868, 958, 1110
AfiI CCNNNNNNNGG 7 cut(s) 28, 134, 329, 446, 651, 754, 1266
AflIII ACRYGT 1 cut(s) 1559
AgsI TTSAA 6 cut(s) 167, 510, 1157, 1200, 1445, 1481
AjnI CCWGG 2 cut(s) 232, 1259
AluBI AGCT 4 cut(s) 587, 666, 1279, 1450
AluI AGCT 4 cut(s) 587, 666, 1279, 1450
Alw21I GWGCWC 2 cut(s) 1452, 1460
Alw26I GTCTC 1 cut(s) 561
AlwI GGATC 3 cut(s) 378, 419, 426
AoxI GGCC 4 cut(s) 19, 253, 570, 799
ApeKI GCWGC 2 cut(s) 1279, 1553
ApoI RAATTY 4 cut(s) 302, 346, 1087, 1358
ArsI GACNNNNNNTTYG 4 cut(s) 308, 340, 377, 409
Asp700I GAANNNNTTC 4 cut(s) 1190, 1485, 1523, 1545
Asp718I GGTACC 1 cut(s) 38
AspS9I GGNCC 3 cut(s) 125, 596, 1437
AsuC2I CCSGG 2 cut(s) 43, 293
AsuHPI GGTGA 2 cut(s) 71, 920
AvaII GGWCC 3 cut(s) 125, 596, 1437
AxyI CCTNAGG 1 cut(s) 129
BaeGI GKGCMC 1 cut(s) 936
BalI TGGCCA 1 cut(s) 255
BanI GGYRCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 1452
BbrPI CACGTG 1 cut(s) 1562
Bbv12I GWGCWC 2 cut(s) 1452, 1460
BbvI GCAGC 2 cut(s) 1266, 1565
BccI CCATC 4 cut(s) 22, 116, 1024, 1229
BceAI ACGGC 1 cut(s) 34
BciT130I CCWGG 2 cut(s) 234, 1261
BcnI CCSGG 2 cut(s) 43, 293
BcoDI GTCTC 1 cut(s) 561
BfaI CTAG 4 cut(s) 84, 354, 419, 429
BfmI CTRYAG 4 cut(s) 646, 898, 1005, 1280
BglI GCCNNNNNGGC 1 cut(s) 551
BisI GCNGC 2 cut(s) 1280, 1554
BlsI GCNGC 2 cut(s) 1281, 1555
Bme1390I CCNGG 4 cut(s) 43, 234, 293, 1261
Bme18I GGWCC 3 cut(s) 125, 596, 1437
BmgT120I GGNCC 3 cut(s) 125, 596, 1437
BmiI GGNNCC 5 cut(s) 40, 597, 598, 1179, 1438
BmrFI CCNGG 4 cut(s) 43, 234, 293, 1261
BmsI GCATC 1 cut(s) 532
Bpu10I CCTNAGC 2 cut(s) 96, 1451
BpuMI CCSGG 2 cut(s) 43, 293
BsaAI YACGTR 1 cut(s) 1562
BsaHI GRCGYC 1 cut(s) 554
BsaJI CCNNGG 2 cut(s) 218, 591
BsaWI WCCGGW 1 cut(s) 1091
BsaXI ACNNNNNCTCC 2 cut(s) 1230, 1260
Bsc4I CCNNNNNNNGG 7 cut(s) 28, 134, 329, 446, 651, 754, 1266
Bse1I ACTGG 2 cut(s) 435, 1504
Bse21I CCTNAGG 1 cut(s) 129
BseBI CCWGG 2 cut(s) 234, 1261
BseDI CCNNGG 2 cut(s) 218, 591
BseGI GGATG 6 cut(s) 33, 127, 622, 706, 983, 1240
BseLI CCNNNNNNNGG 7 cut(s) 28, 134, 329, 446, 651, 754, 1266
BseMII CTCAG 4 cut(s) 120, 480, 597, 1465
BseNI ACTGG 2 cut(s) 435, 1504
BseRI GAGGAG 1 cut(s) 379
BseSI GKGCMC 1 cut(s) 936
BseXI GCAGC 2 cut(s) 1266, 1565
Bsh1236I CGCG 1 cut(s) 1267
BshFI GGCC 4 cut(s) 21, 255, 572, 801
BshNI GGYRCC 1 cut(s) 38
BsiHKAI GWGCWC 2 cut(s) 1452, 1460
BsiSI CCGG 3 cut(s) 42, 292, 1092
BslFI GGGAC 2 cut(s) 582, 1450
BslI CCNNNNNNNGG 7 cut(s) 28, 134, 329, 446, 651, 754, 1266
BsmAI GTCTC 1 cut(s) 561
BsmBI CGTCTC 1 cut(s) 561
BsmFI GGGAC 2 cut(s) 582, 1450
BsnI GGCC 4 cut(s) 21, 255, 572, 801
Bsp1286I GDGCHC 3 cut(s) 936, 1452, 1460
Bsp143I GATC 3 cut(s) 370, 424, 431
BspACI CCGC 3 cut(s) 975, 1267, 1528
BspANI GGCC 4 cut(s) 21, 255, 572, 801
BspCNI CTCAG 4 cut(s) 121, 481, 596, 1464
BspFNI CGCG 1 cut(s) 1267
BspLI GGNNCC 5 cut(s) 40, 597, 598, 1179, 1438
BspMAI CTGCAG 2 cut(s) 1009, 1284
BspPI GGATC 3 cut(s) 378, 419, 426
BspT107I GGYRCC 1 cut(s) 38
BsrI ACTGG 2 cut(s) 435, 1504
BssECI CCNNGG 2 cut(s) 218, 591
BssMI GATC 3 cut(s) 370, 424, 431
BssNAI GTATAC 1 cut(s) 1254
BssNI GRCGYC 1 cut(s) 554
Bst1107I GTATAC 1 cut(s) 1254
Bst2UI CCWGG 2 cut(s) 234, 1261
Bst4CI ACNGT 4 cut(s) 69, 449, 1060, 1441
Bst6I CTCTTC 2 cut(s) 855, 1584
BstACI GRCGYC 1 cut(s) 554
BstBAI YACGTR 1 cut(s) 1562
BstC8I GCNNGC 1 cut(s) 500
BstDEI CTNAG 6 cut(s) 96, 129, 489, 583, 1170, 1451
BstDSI CCRYGG 2 cut(s) 218, 591
BstENI CCTNNNNNAGG 2 cut(s) 132, 649
BstF5I GGATG 6 cut(s) 33, 127, 622, 706, 983, 1240
BstFNI CGCG 1 cut(s) 1267
BstKTI GATC 3 cut(s) 373, 427, 434
BstMAI GTCTC 1 cut(s) 561
BstMBI GATC 3 cut(s) 370, 424, 431
BstMWI GCNNNNNNNGC 3 cut(s) 551, 893, 1213
BstNI CCWGG 2 cut(s) 234, 1261
BstNSI RCATGY 2 cut(s) 502, 768
BstSCI CCNGG 4 cut(s) 41, 232, 291, 1259
BstSFI CTRYAG 4 cut(s) 646, 898, 1005, 1280
BstSLI GKGCMC 1 cut(s) 936
BstUI CGCG 1 cut(s) 1267
BstV1I GCAGC 2 cut(s) 1266, 1565
BstX2I RGATCY 3 cut(s) 370, 424, 431
BstYI RGATCY 3 cut(s) 370, 424, 431
BstZ17I GTATAC 1 cut(s) 1254
Bsu36I CCTNAGG 1 cut(s) 129
BsuRI GGCC 4 cut(s) 21, 255, 572, 801
BtgI CCRYGG 2 cut(s) 218, 591
BtsCI GGATG 6 cut(s) 33, 127, 622, 706, 983, 1240
BtsIMutI CAGTG 2 cut(s) 65, 116
Cac8I GCNNGC 1 cut(s) 500
Cfr13I GGNCC 3 cut(s) 125, 596, 1437
CseI GACGC 2 cut(s) 543, 1355
Csp6I GTAC 4 cut(s) 39, 867, 957, 1109
CspCI CAANNNNNGTGG 2 cut(s) 1282, 1317
CviAII CATG 8 cut(s) 118, 499, 547, 634, 765, 884, 893, 1370
CviQI GTAC 4 cut(s) 39, 867, 957, 1109
DdeI CTNAG 6 cut(s) 96, 129, 489, 583, 1170, 1451
DpnI GATC 3 cut(s) 372, 426, 433
DpnII GATC 3 cut(s) 370, 424, 431
DraI TTTAAA 1 cut(s) 51
EaeI YGGCCR 2 cut(s) 253, 799
Eam1104I CTCTTC 2 cut(s) 855, 1584
EarI CTCTTC 2 cut(s) 855, 1584
EciI GGCGGA 2 cut(s) 990, 1517
Ecl136II GAGCTC 1 cut(s) 1450
Eco24I GRGCYC 1 cut(s) 1452
Eco32I GATATC 1 cut(s) 141
Eco47I GGWCC 3 cut(s) 125, 596, 1437
Eco53kI GAGCTC 1 cut(s) 1450
Eco57I CTGAAG 1 cut(s) 1553
Eco72I CACGTG 1 cut(s) 1562
Eco81I CCTNAGG 1 cut(s) 129
EcoICRI GAGCTC 1 cut(s) 1450
EcoNI CCTNNNNNAGG 2 cut(s) 132, 649
EcoO109I RGGNCCY 1 cut(s) 596
EcoRII CCWGG 2 cut(s) 232, 1259
EcoRV GATATC 1 cut(s) 141
EcoT22I ATGCAT 1 cut(s) 1354
EcoT38I GRGCYC 1 cut(s) 1452
Esp3I CGTCTC 1 cut(s) 561
FaeI CATG 8 cut(s) 121, 502, 550, 637, 768, 887, 896, 1373
FaqI GGGAC 2 cut(s) 582, 1450
FatI CATG 8 cut(s) 117, 498, 546, 633, 764, 883, 892, 1369
FauI CCCGC 1 cut(s) 1260
FauNDI CATATG 1 cut(s) 1354
FblI GTMKAC 1 cut(s) 1253
Fnu4HI GCNGC 2 cut(s) 1280, 1554
FokI GGATG 6 cut(s) 40, 134, 629, 713, 990, 1247
FriOI GRGCYC 1 cut(s) 1452
Fsp4HI GCNGC 2 cut(s) 1280, 1554
FspBI CTAG 4 cut(s) 84, 354, 419, 429
GluI GCNGC 2 cut(s) 1280, 1554
HaeIII GGCC 4 cut(s) 21, 255, 572, 801
HapII CCGG 3 cut(s) 42, 292, 1092
HgaI GACGC 2 cut(s) 543, 1355
Hin1I GRCGYC 1 cut(s) 554
Hin1II CATG 8 cut(s) 121, 502, 550, 637, 768, 887, 896, 1373
HincII GTYRAC 2 cut(s) 1037, 1221
HindII GTYRAC 2 cut(s) 1037, 1221
HinfI GANTC 5 cut(s) 13, 386, 485, 889, 1541
HpaII CCGG 3 cut(s) 42, 292, 1092
HphI GGTGA 2 cut(s) 71, 920
Hpy166II GTNNAC 4 cut(s) 1037, 1221, 1254, 1565
Hpy188I TCNGA 7 cut(s) 12, 490, 762, 1029, 1185, 1491, 1518
Hpy188III TCNNGA 6 cut(s) 84, 128, 192, 1141, 1445, 1453
Hpy8I GTNNAC 4 cut(s) 1037, 1221, 1254, 1565
HpyAV CCTTC 5 cut(s) 50, 1168, 1206, 1267, 1534
HpyCH4III ACNGT 4 cut(s) 69, 449, 1060, 1441
HpyCH4IV ACGT 3 cut(s) 521, 1166, 1561
HpyCH4V TGCA 8 cut(s) 206, 768, 1007, 1097, 1282, 1329, 1352, 1380
HpyF10VI GCNNNNNNNGC 3 cut(s) 551, 893, 1213
HpyF3I CTNAG 6 cut(s) 96, 129, 489, 583, 1170, 1451
HpySE526I ACGT 3 cut(s) 521, 1166, 1561
Hsp92I GRCGYC 1 cut(s) 554
Hsp92II CATG 8 cut(s) 121, 502, 550, 637, 768, 887, 896, 1373
KflI GGGWCCC 1 cut(s) 596
KpnI GGTACC 1 cut(s) 42
Kzo9I GATC 3 cut(s) 370, 424, 431
LmnI GCTCC 2 cut(s) 1276, 1455
Lsp1109I GCAGC 2 cut(s) 1266, 1565
LweI GCATC 1 cut(s) 532
MaeI CTAG 4 cut(s) 84, 354, 419, 429
MaeII ACGT 3 cut(s) 521, 1166, 1561
MaeIII GTNAC 2 cut(s) 776, 908
MalI GATC 3 cut(s) 372, 426, 433
MboI GATC 3 cut(s) 370, 424, 431
MboII GAAGA 4 cut(s) 72, 434, 872, 1601
MflI RGATCY 3 cut(s) 370, 424, 431
MhlI GDGCHC 3 cut(s) 936, 1452, 1460
MlsI TGGCCA 1 cut(s) 255
MluCI AATT 9 cut(s) 76, 87, 257, 302, 346, 442, 841, 1087, 1358
MluNI TGGCCA 1 cut(s) 255
MlyI GAGTC 3 cut(s) 380, 479, 883
MmeI TCCRAC 1 cut(s) 310
Mox20I TGGCCA 1 cut(s) 255
Mph1103I ATGCAT 1 cut(s) 1354
MroXI GAANNNNTTC 4 cut(s) 1190, 1485, 1523, 1545
MscI TGGCCA 1 cut(s) 255
MseI TTAA 7 cut(s) 50, 200, 363, 690, 752, 1104, 1605
MslI CAYNNNNRTG 2 cut(s) 632, 674
Msp20I TGGCCA 1 cut(s) 255
MspI CCGG 3 cut(s) 42, 292, 1092
MspR9I CCNGG 4 cut(s) 43, 234, 293, 1261
MssI GTTTAAAC 1 cut(s) 51
MvaI CCWGG 2 cut(s) 234, 1261
MvnI CGCG 1 cut(s) 1267
MwoI GCNNNNNNNGC 3 cut(s) 551, 893, 1213
NciI CCSGG 2 cut(s) 43, 293
NdeI CATATG 1 cut(s) 1354
NdeII GATC 3 cut(s) 370, 424, 431
NlaIII CATG 8 cut(s) 121, 502, 550, 637, 768, 887, 896, 1373
NlaIV GGNNCC 5 cut(s) 40, 597, 598, 1179, 1438
NmuCI GTSAC 2 cut(s) 776, 908
NsiI ATGCAT 1 cut(s) 1354
NspI RCATGY 2 cut(s) 502, 768
PaeI GCATGC 1 cut(s) 502
PcsI WCGNNNNNNNCGW 1 cut(s) 320
PdmI GAANNNNTTC 4 cut(s) 1190, 1485, 1523, 1545
PfeI GAWTC 2 cut(s) 13, 1541
PkrI GCNGC 2 cut(s) 1281, 1555
PleI GAGTC 3 cut(s) 380, 479, 883
PmaCI CACGTG 1 cut(s) 1562
PmeI GTTTAAAC 1 cut(s) 51
PmlI CACGTG 1 cut(s) 1562
PpsI GAGTC 3 cut(s) 380, 479, 883
Ppu21I YACGTR 1 cut(s) 1562
PpuMI RGGWCCY 1 cut(s) 596
PsiI TTATAA 2 cut(s) 606, 942
Psp124BI GAGCTC 1 cut(s) 1452
Psp5II RGGWCCY 1 cut(s) 596
Psp6I CCWGG 2 cut(s) 232, 1259
PspCI CACGTG 1 cut(s) 1562
PspGI CCWGG 2 cut(s) 232, 1259
PspN4I GGNNCC 5 cut(s) 40, 597, 598, 1179, 1438
PspPI GGNCC 3 cut(s) 125, 596, 1437
PspPPI RGGWCCY 1 cut(s) 596
PstI CTGCAG 2 cut(s) 1009, 1284
PsuI RGATCY 3 cut(s) 370, 424, 431
RsaI GTAC 4 cut(s) 40, 868, 958, 1110
RsaNI GTAC 4 cut(s) 39, 867, 957, 1109
RseI CAYNNNNRTG 2 cut(s) 632, 674
SacI GAGCTC 1 cut(s) 1452
SaqAI TTAA 7 cut(s) 50, 200, 363, 690, 752, 1104, 1605
SatI GCNGC 2 cut(s) 1280, 1554
Sau3AI GATC 3 cut(s) 370, 424, 431
Sau96I GGNCC 3 cut(s) 125, 596, 1437
SchI GAGTC 3 cut(s) 380, 479, 883
ScrFI CCNGG 4 cut(s) 43, 234, 293, 1261
SduI GDGCHC 3 cut(s) 936, 1452, 1460
SfaNI GCATC 1 cut(s) 532
SfcI CTRYAG 4 cut(s) 646, 898, 1005, 1280
SinI GGWCC 3 cut(s) 125, 596, 1437
SmiMI CAYNNNNRTG 2 cut(s) 632, 674
SphI GCATGC 1 cut(s) 502
Sse9I AATT 9 cut(s) 76, 87, 257, 302, 346, 442, 841, 1087, 1358
SsiI CCGC 3 cut(s) 975, 1267, 1528
SspMI CTAG 4 cut(s) 84, 354, 419, 429
SstI GAGCTC 1 cut(s) 1452
StyD4I CCNGG 4 cut(s) 41, 232, 291, 1259
TaaI ACNGT 4 cut(s) 69, 449, 1060, 1441
TaiI ACGT 3 cut(s) 524, 1169, 1564
TasI AATT 9 cut(s) 76, 87, 257, 302, 346, 442, 841, 1087, 1358
TfiI GAWTC 2 cut(s) 13, 1541
Tru1I TTAA 7 cut(s) 50, 200, 363, 690, 752, 1104, 1605
Tru9I TTAA 7 cut(s) 50, 200, 363, 690, 752, 1104, 1605
TscAI CASTG 2 cut(s) 72, 116
TseFI GTSAC 2 cut(s) 776, 908
TseI GCWGC 2 cut(s) 1279, 1553
Tsp45I GTSAC 2 cut(s) 776, 908
TspDTI ATGAA 7 cut(s) 106, 359, 616, 658, 717, 1035, 1371
TspGWI ACGGA 4 cut(s) 207, 312, 350, 865
TspRI CASTG 2 cut(s) 72, 116
VpaK11BI GGWCC 3 cut(s) 125, 596, 1437
XagI CCTNNNNNAGG 2 cut(s) 132, 649
XapI RAATTY 4 cut(s) 302, 346, 1087, 1358
XbaI TCTAGA 1 cut(s) 83
XceI RCATGY 2 cut(s) 502, 768
XmiI GTMKAC 1 cut(s) 1253
XmnI GAANNNNTTC 4 cut(s) 1190, 1485, 1523, 1545
XspI CTAG 4 cut(s) 84, 354, 419, 429
Zsp2I ATGCAT 1 cut(s) 1354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.