pycom12g13270

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009150
Physical Location & Seq
Reverse (-)
15718284 .. 15724159
5876 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2538 bp
ATGGGAGAAGAGGCAATTTGTGTGGGGAAATTGAGAGATGGGGAAGTGGAAACAGAAAACTTCCCGAGAACTGAACTGAAGCGAGAGCACGAGTGTCTCGAAGATGATAGCGGGGCAGACGGGTTTCCAAACAAGAAGCAAGTGAAGGAGCATTCCAATGAGGATATAAGATCGGAAGTATCAAACCCCGTTGTATCTCCGAAAGAGAATGGCTCCATTTTTCAGGACATTACTAGCCAACCTGCGGAATTGGCAAATAGTAACGGAACGGAGTGTGGTGAAGTTGAGTCCTCATGTTCGGAGAATTTGGGTGCAGAGAACTTGAGCAATGGACAGTGTACCGAGAATGATAATTGTCAAATTGAGAACGAGACGAATGGTGATGTATTGACCTCTCGTGTTGTGGTAGAAATTCCTAAGCTTGCCAGTTCATCTGGGATTCGCAAGATTACGTTCAAGTTCAGTAAGAAAAAGGAGAATCAGACTGCGAGTAATCAAGACTTTTCACATGGAGGTTCTCACGAGGAGCCCGGAATGGACTTTCAAGCGATGCCTAGTACAAGTAGGGAGTTTCCAGATAGTTCATATTGGAGGCAATGCGCTGAAACTGGCAACTTTCATCCTTGTGCGCTTAACTCAAAGGTGGAAAGTTCTAACTATGTAGTTCCGAGCAGCTATCCTTCAAATGTTAAGAAGCTATTGTCTACTGGTATTCTTGATGGAGCTCGAGTGAAGTATGTTTCCTCCCCGCCAAAGATAGAGCTACATGGAATTGTAAGTGGCGGTGGTTATTTGTGCGGCTGCTCATCATGCAATTTCACCAGGGTACTCAGTGCATATGAGTTTGAGCAGCATGCTGGCGTCAAGACTCGGCATCCTAATAATCACATATACATGGAGAATGGGAGGGCAGTTTATAGCATCATACAAGAACTGAAGACTGCTCCGCTAAGCAGTCTGGATGAAGTGATAAAGGATATTGCTGGTTCTGCTCTCAATGAAGAGTCCTTCCGGGTTTGGAAAGAAACTCTTAATCGAAGTGATGGAATGGCTGAAGTGGAGAAAAGACATCGTGTGAAGCTTCCTAAATTGCGTCATCCAATTCCCAGACCAAGCTTCCACAATCCGTATGCAGCTATGTACCAGAAGAAAACTGCTGAAGGTGGCAATAGGAAAAGGGATAATGATCTGCACCGGTTACTATTTATGCCAAATGGACTTCCAGATGGAGCTAAATTGGCATACTATGTCAAAGGGCAGAGATTACTTAGCGGCTATAAGCAGGGAAATGGGATAGTCTGTAATTGCTGTGACAAAGAGATAAGCCCTTCACAGTTTGAAGCTCATGCTGGAATGGCTGCAAGGCGTCAACCCTACCGTCACATCTATATTTCCAATGGACTGACGCTTCACGATATAGCCATGTCATTGGCCAACGGCCAAAACCTGACAATTGGTGGCAGTGATGGCAATGATGATATGTGTGCTGTATGTGGACATGATACAGGGGATATGATTTTCTGTGATGGATGCCCTCGTGCTTATCATTCAGCTTGTTTGGATTTACCACGGGTTCCTCAAGGTGATTGGCATTGTCCAGATTGTAGAGATAAGTTTGAACCTGGTAAAAAAGCTGCTGCTGGGGAATCTTCAAATTTTGGAAGACCCATTGTAATACGGTTGACACGGGTCTTCAATGCACCTGAGTATGAAATTGGTGGCTGCGTCGTTTGCAGGAGCCATGATTTCAGTGTGGCTTTATTTGATGAACGAACTGTTATAATCTGTGATCAGTGTGAGAAGGAGTTCCATGTTGGCTGCTTACGGAACAGTGGATTATGTGACTTAAAAGCACTCCCCAAAGATAAATGGTTTTGTTGTGATGACTGTAACAGGATTCATGCAGCTTTACAGAATTTAGTACTCGGTGAAGCAGAGCGTGTTCCAGCTACATTATCAGATACAATAATCCAGAGTGGAAATGCAGATAGAGGTATATTTATTGACGGAATGGCAGATGTTCAATGGCGAGTTTTCAGTGGAAAGAGGCGCTATCCGGAACACCTACCCTTCCTTTCGAGGGCTGCTGCTATTTTTCGAGAGTGCTTTGACCCTATTGTTGCAGAGTCTGGTCGTGACTTGATCCCTGTTATGGTATATGGGAGAAATATTTCAGGTCAAGAGTTTGGAGGCATGTACTGTGTAGTTTTAATAGTGGGGTCTGTTGTTGTATCTGCTGGTCTCCTTAGGGTTTTTGGTCGGGAGGTTGCTGAGCTTCCTATTGTTGCTACTAGTAGGGAACATCAAGGAAAAGGTTATTTCCAAGCATTATTTTCATGTATGGAGAGTTTACTTATTTCTCTGAAAGTGGAAAAACTGGTTCTCCCCGCGGCTGAGGAAGCTGAGTCAATCTGGACGAGGAAATTAGGATTTAGAAAGATGAGAGACGAGCAATTATCGAAATACATGAGGGAAGTGCAGTTGACAATTTTCAGGGGGACGTCAATGCTAGAAAAGGTGGTGAAGTTGACGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD

Protein Analysis

846

Amino Acids

93.62

Weight (kDa)

6.05

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 257 - 311 2.2e-13 Tify domain binding domain
TDBD PF16135 428 - 473 1e-13 Tify domain binding domain
PHD-1st_NSD PF23011 492 - 536 7.1e-07 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD PF00628 495 - 535 1.3e-08 PHD-finger
IDM1_C PF23209 703 - 839 2.2e-50 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1781
AatII GACGTC 1 cut(s) 2504
Acc36I ACCTGC 1 cut(s) 250
AccI GTMKAC 1 cut(s) 704
AccII CGCG 1 cut(s) 2390
AccIII TCCGGA 1 cut(s) 2056
AciI CCGC 9 cut(s) 111, 245, 749, 783, 798, 947, 1272, 2388, 2390
AclWI GGATC 1 cut(s) 2137
AcoI YGGCCR 2 cut(s) 1431, 1438
AcsI RAATTY 4 cut(s) 304, 411, 1654, 1915
AcuI CTGAAG 4 cut(s) 98, 956, 1074, 1179
AcyI GRCGYC 3 cut(s) 861, 1366, 2501
AfaI GTAC 6 cut(s) 340, 559, 828, 1142, 1923, 2198
AfiI CCNNNNNNNGG 3 cut(s) 244, 2080, 2152
AgeI ACCGGT 1 cut(s) 1194
AgsI TTSAA 8 cut(s) 457, 545, 684, 1340, 1619, 1653, 1696, 2024
AhlI ACTAGT 1 cut(s) 2291
AjnI CCWGG 2 cut(s) 821, 1621
Alw21I GWGCWC 2 cut(s) 90, 727
Alw26I GTCTC 4 cut(s) 101, 365, 2246, 2439
AlwI GGATC 1 cut(s) 2137
AlwNI CAGNNNCTG 1 cut(s) 2129
Ama87I CYCGRG 2 cut(s) 64, 726
Aor13HI TCCGGA 1 cut(s) 2056
AoxI GGCC 2 cut(s) 1431, 1438
ApoI RAATTY 4 cut(s) 304, 411, 1654, 1915
AsiGI ACCGGT 1 cut(s) 1194
Asp700I GAANNNNTTC 2 cut(s) 1805, 2170
AspLEI GCGC 3 cut(s) 602, 631, 2052
AsuC2I CCSGG 2 cut(s) 531, 1013
AsuHPI GGTGA 6 cut(s) 290, 392, 811, 1595, 1940, 2533
AvaI CYCGRG 2 cut(s) 64, 726
AxyI CCTNAGG 1 cut(s) 2246
BalI TGGCCA 1 cut(s) 1433
BanII GRGCYC 2 cut(s) 531, 727
BauI CACGAG 4 cut(s) 89, 396, 521, 1536
BbsI GAAGAC 3 cut(s) 944, 1669, 1684
Bbv12I GWGCWC 2 cut(s) 90, 727
BbvCI CCTCAGC 1 cut(s) 2394
BccI CCATC 6 cut(s) 32, 713, 1037, 1220, 1460, 1520
BceAI ACGGC 1 cut(s) 1453
BciT130I CCWGG 2 cut(s) 823, 1623
BclI TGATCA 1 cut(s) 1789
BcnI CCSGG 2 cut(s) 531, 1013
BcoDI GTCTC 4 cut(s) 101, 365, 2246, 2439
BcuI ACTAGT 1 cut(s) 2291
BfaI CTAG 4 cut(s) 234, 555, 2292, 2510
BfoI RGCGCY 1 cut(s) 2053
BfuAI ACCTGC 1 cut(s) 250
BlpI GCTNAGC 2 cut(s) 950, 2271
BmcAI AGTACT 1 cut(s) 1923
Bme1390I CCNGG 4 cut(s) 531, 823, 1013, 1623
BmeT110I CYCGRG 2 cut(s) 64, 726
BmiI GGNNCC 4 cut(s) 214, 528, 1575, 1739
BmrFI CCNGG 4 cut(s) 531, 823, 1013, 1623
BmsI GCATC 4 cut(s) 540, 883, 930, 1520
BoxI GACNNNNGTC 1 cut(s) 1688
BpiI GAAGAC 3 cut(s) 944, 1669, 1684
BplI GAGNNNNNCTC 2 cut(s) 197, 229
Bpu10I CCTNAGC 2 cut(s) 417, 2394
Bpu1102I GCTNAGC 2 cut(s) 950, 2271
BpuEI CTTGAG 2 cut(s) 343, 1563
BpuMI CCSGG 2 cut(s) 531, 1013
BsaBI GATNNNNATC 1 cut(s) 1185
BsaHI GRCGYC 3 cut(s) 861, 1366, 2501
BsaI GGTCTC 1 cut(s) 2246
BsaJI CCNNGG 3 cut(s) 822, 1568, 2388
BsaWI WCCGGW 2 cut(s) 1194, 2056
BsaXI ACNNNNNCTCC 4 cut(s) 714, 744, 2367, 2397
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 2080, 2152
Bse118I RCCGGY 1 cut(s) 1194
Bse1I ACTGG 4 cut(s) 426, 613, 712, 2382
Bse21I CCTNAGG 1 cut(s) 2246
Bse3DI GCAATG 3 cut(s) 334, 602, 1477
Bse8I GATNNNNATC 1 cut(s) 1185
BseAI TCCGGA 1 cut(s) 2056
BseBI CCWGG 2 cut(s) 823, 1623
BseDI CCNNGG 3 cut(s) 822, 1568, 2388
BseGI GGATG 5 cut(s) 619, 874, 967, 1096, 1535
BseJI GATNNNNATC 1 cut(s) 1185
BseLI CCNNNNNNNGG 3 cut(s) 244, 2080, 2152
BseMI GCAATG 3 cut(s) 334, 602, 1477
BseMII CTCAG 5 cut(s) 844, 1695, 2262, 2385, 2394
BseNI ACTGG 4 cut(s) 426, 613, 712, 2382
BseRI GAGGAG 1 cut(s) 539
BseYI CCCAGC 1 cut(s) 1640
BsgI GTGCAG 3 cut(s) 333, 1175, 2498
Bsh1236I CGCG 1 cut(s) 2390
BshFI GGCC 2 cut(s) 1433, 1440
BshTI ACCGGT 1 cut(s) 1194
BsiHKAI GWGCWC 2 cut(s) 90, 727
BsiHKCI CYCGRG 2 cut(s) 64, 726
BsiSI CCGG 4 cut(s) 531, 1012, 1195, 2057
BslFI GGGAC 1 cut(s) 2512
BslI CCNNNNNNNGG 3 cut(s) 244, 2080, 2152
BsmAI GTCTC 4 cut(s) 101, 365, 2246, 2439
BsmBI CGTCTC 2 cut(s) 365, 2439
BsmFI GGGAC 1 cut(s) 2512
BsmI GAATGC 1 cut(s) 151
BsnI GGCC 2 cut(s) 1433, 1440
Bso31I GGTCTC 1 cut(s) 2246
BsoBI CYCGRG 2 cut(s) 64, 726
Bsp1286I GDGCHC 3 cut(s) 90, 531, 727
Bsp13I TCCGGA 1 cut(s) 2056
Bsp143I GATC 4 cut(s) 170, 1186, 1789, 2142
Bsp1720I GCTNAGC 2 cut(s) 950, 2271
BspACI CCGC 9 cut(s) 111, 245, 749, 783, 798, 947, 1272, 2388, 2390
BspANI GGCC 2 cut(s) 1433, 1440
BspCNI CTCAG 5 cut(s) 843, 1696, 2263, 2386, 2395
BspEI TCCGGA 1 cut(s) 2056
BspFNI CGCG 1 cut(s) 2390
BspLI GGNNCC 4 cut(s) 214, 528, 1575, 1739
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 1 cut(s) 2137
BspTNI GGTCTC 1 cut(s) 2246
BsrDI GCAATG 3 cut(s) 334, 602, 1477
BsrFI RCCGGY 1 cut(s) 1194
BsrI ACTGG 4 cut(s) 426, 613, 712, 2382
BssAI RCCGGY 1 cut(s) 1194
BssECI CCNNGG 3 cut(s) 822, 1568, 2388
BssMI GATC 4 cut(s) 170, 1186, 1789, 2142
BssNI GRCGYC 3 cut(s) 861, 1366, 2501
BssSI CACGAG 4 cut(s) 89, 396, 521, 1536
Bst2BI CACGAG 4 cut(s) 89, 396, 521, 1536
Bst2UI CCWGG 2 cut(s) 823, 1623
Bst4CI ACNGT 8 cut(s) 336, 1335, 1379, 1680, 1777, 1832, 1889, 2201
Bst6I CTCTTC 2 cut(s) 3, 996
BstACI GRCGYC 3 cut(s) 861, 1366, 2501
BstC8I GCNNGC 3 cut(s) 423, 855, 859
BstDEI CTNAG 9 cut(s) 417, 830, 950, 1268, 1704, 2246, 2271, 2394, 2403
BstDSI CCRYGG 2 cut(s) 1568, 2388
BstF5I GGATG 5 cut(s) 619, 874, 967, 1096, 1535
BstFNI CGCG 1 cut(s) 2390
BstH2I RGCGCY 1 cut(s) 2053
BstHHI GCGC 3 cut(s) 602, 631, 2052
BstKTI GATC 4 cut(s) 173, 1189, 1792, 2145
BstMAI GTCTC 4 cut(s) 101, 365, 2246, 2439
BstMBI GATC 4 cut(s) 170, 1186, 1789, 2142
BstMWI GCNNNNNNNGC 8 cut(s) 251, 810, 989, 1238, 1355, 1467, 1731, 2399
BstNI CCWGG 2 cut(s) 823, 1623
BstNSI RCATGY 2 cut(s) 857, 2197
BstPAI GACNNNNGTC 1 cut(s) 1688
BstSCI CCNGG 4 cut(s) 529, 821, 1011, 1621
BstUI CGCG 1 cut(s) 2390
BstV2I GAAGAC 3 cut(s) 944, 1669, 1684
BstXI CCANNNNNNTGG 1 cut(s) 1429
Bsu36I CCTNAGG 1 cut(s) 2246
BsuRI GGCC 2 cut(s) 1433, 1440
BtgI CCRYGG 2 cut(s) 1568, 2388
BtgZI GCGATG 1 cut(s) 563
BtsCI GGATG 5 cut(s) 619, 874, 967, 1096, 1535
BtsI GCAGTG 1 cut(s) 1468
BtsIMutI CAGTG 7 cut(s) 341, 838, 1468, 1756, 1799, 1837, 2044
BveI ACCTGC 1 cut(s) 250
Cac8I GCNNGC 3 cut(s) 423, 855, 859
CaiI CAGNNNCTG 1 cut(s) 2129
CfoI GCGC 3 cut(s) 602, 631, 2052
Cfr10I RCCGGY 1 cut(s) 1194
Cfr42I CCGCGG 1 cut(s) 2391
CseI GACGC 5 cut(s) 850, 1082, 1355, 1414, 1714
CsiI ACCWGGT 1 cut(s) 1621
Csp6I GTAC 6 cut(s) 339, 558, 827, 1141, 1922, 2197
CspAI ACCGGT 1 cut(s) 1194
CspCI CAANNNNNGTGG 1 cut(s) 38
CviQI GTAC 6 cut(s) 339, 558, 827, 1141, 1922, 2197
DdeI CTNAG 9 cut(s) 417, 830, 950, 1268, 1704, 2246, 2271, 2394, 2403
DpnI GATC 4 cut(s) 172, 1188, 1791, 2144
DpnII GATC 4 cut(s) 170, 1186, 1789, 2142
EaeI YGGCCR 2 cut(s) 1431, 1438
Eam1104I CTCTTC 2 cut(s) 3, 996
EarI CTCTTC 2 cut(s) 3, 996
Ecl136II GAGCTC 1 cut(s) 725
Eco24I GRGCYC 2 cut(s) 531, 727
Eco31I GGTCTC 1 cut(s) 2246
Eco53kI GAGCTC 1 cut(s) 725
Eco57I CTGAAG 4 cut(s) 98, 956, 1074, 1179
Eco81I CCTNAGG 1 cut(s) 2246
Eco88I CYCGRG 2 cut(s) 64, 726
EcoICRI GAGCTC 1 cut(s) 725
EcoRII CCWGG 2 cut(s) 821, 1621
EcoT38I GRGCYC 2 cut(s) 531, 727
Esp3I CGTCTC 2 cut(s) 365, 2439
FalI AAGNNNNNCTT 2 cut(s) 1014, 1046
FaqI GGGAC 1 cut(s) 2512
FauI CCCGC 3 cut(s) 104, 756, 2395
FauNDI CATATG 1 cut(s) 838
FbaI TGATCA 1 cut(s) 1789
FblI GTMKAC 1 cut(s) 704
FokI GGATG 5 cut(s) 606, 861, 974, 1083, 1542
FriOI GRGCYC 2 cut(s) 531, 727
FspBI CTAG 4 cut(s) 234, 555, 2292, 2510
GlaI GCGC 3 cut(s) 601, 630, 2051
GsaI CCCAGC 1 cut(s) 1644
HaeII RGCGCY 1 cut(s) 2053
HaeIII GGCC 2 cut(s) 1433, 1440
HapII CCGG 4 cut(s) 531, 1012, 1195, 2057
HgaI GACGC 5 cut(s) 850, 1082, 1355, 1414, 1714
HhaI GCGC 3 cut(s) 602, 631, 2052
Hin1I GRCGYC 3 cut(s) 861, 1366, 2501
Hin6I GCGC 3 cut(s) 600, 629, 2050
HinP1I GCGC 3 cut(s) 600, 629, 2050
HincII GTYRAC 4 cut(s) 1370, 1683, 2484, 2529
HindII GTYRAC 4 cut(s) 1370, 1683, 2484, 2529
HindIII AAGCTT 3 cut(s) 419, 1079, 1114
HinfI GANTC 9 cut(s) 287, 439, 478, 868, 1004, 1646, 1897, 2126, 2405
HpaII CCGG 4 cut(s) 531, 1012, 1195, 2057
HphI GGTGA 6 cut(s) 290, 392, 811, 1595, 1940, 2533
Hpy166II GTNNAC 8 cut(s) 339, 705, 1370, 1496, 1683, 2351, 2484, 2529
Hpy188I TCNGA 7 cut(s) 175, 201, 301, 483, 669, 1960, 2364
Hpy8I GTNNAC 8 cut(s) 339, 705, 1370, 1496, 1683, 2351, 2484, 2529
Hpy99I CGWCG 1 cut(s) 1730
HpyAV CCTTC 7 cut(s) 139, 690, 1018, 1154, 1338, 1795, 2080
HpyCH4III ACNGT 8 cut(s) 336, 1335, 1379, 1680, 1777, 1832, 1889, 2201
HpyCH4IV ACGT 2 cut(s) 452, 2501
HpyF10VI GCNNNNNNNGC 8 cut(s) 251, 810, 989, 1238, 1355, 1467, 1731, 2399
HpyF3I CTNAG 9 cut(s) 417, 830, 950, 1268, 1704, 2246, 2271, 2394, 2403
HpySE526I ACGT 2 cut(s) 452, 2501
Hsp92I GRCGYC 3 cut(s) 861, 1366, 2501
HspAI GCGC 3 cut(s) 600, 629, 2050
Kpn2I TCCGGA 1 cut(s) 2056
Ksp22I TGATCA 1 cut(s) 1789
KspI CCGCGG 1 cut(s) 2391
Kzo9I GATC 4 cut(s) 170, 1186, 1789, 2142
LmnI GCTCC 7 cut(s) 148, 218, 526, 722, 949, 1229, 1737
LweI GCATC 4 cut(s) 540, 883, 930, 1520
MabI ACCWGGT 1 cut(s) 1621
MaeI CTAG 4 cut(s) 234, 555, 2292, 2510
MaeII ACGT 2 cut(s) 452, 2501
MaeIII GTNAC 7 cut(s) 260, 1197, 1310, 1379, 1841, 1889, 2135
MalI GATC 4 cut(s) 172, 1188, 1791, 2144
MboI GATC 4 cut(s) 170, 1186, 1789, 2142
MboII GAAGA 8 cut(s) 20, 113, 949, 1013, 1159, 1641, 1674, 1684
MfeI CAATTG 1 cut(s) 1452
MhlI GDGCHC 3 cut(s) 90, 531, 727
MlsI TGGCCA 1 cut(s) 1433
MluNI TGGCCA 1 cut(s) 1433
MlyI GAGTC 5 cut(s) 296, 862, 1013, 2135, 2414
Mox20I TGGCCA 1 cut(s) 1433
MroI TCCGGA 1 cut(s) 2056
MroXI GAANNNNTTC 2 cut(s) 1805, 2170
MscI TGGCCA 1 cut(s) 1433
MseI TTAA 5 cut(s) 633, 690, 1032, 1847, 2210
MslI CAYNNNNRTG 3 cut(s) 156, 624, 893
Msp20I TGGCCA 1 cut(s) 1433
MspA1I CMGCKG 1 cut(s) 2390
MspI CCGG 4 cut(s) 531, 1012, 1195, 2057
MspR9I CCNGG 4 cut(s) 531, 823, 1013, 1623
MunI CAATTG 1 cut(s) 1452
Mva1269I GAATGC 1 cut(s) 151
MvaI CCWGG 2 cut(s) 823, 1623
MvnI CGCG 1 cut(s) 2390
MwoI GCNNNNNNNGC 8 cut(s) 251, 810, 989, 1238, 1355, 1467, 1731, 2399
NciI CCSGG 2 cut(s) 531, 1013
NdeI CATATG 1 cut(s) 838
NdeII GATC 4 cut(s) 170, 1186, 1789, 2142
NlaIV GGNNCC 4 cut(s) 214, 528, 1575, 1739
NmeAIII GCCGAG 1 cut(s) 850
NmuCI GTSAC 4 cut(s) 1310, 1379, 1841, 2135
NspI RCATGY 2 cut(s) 857, 2197
PaeI GCATGC 1 cut(s) 857
PaeR7I CTCGAG 1 cut(s) 726
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 1 cut(s) 151
PdmI GAANNNNTTC 2 cut(s) 1805, 2170
PfeI GAWTC 4 cut(s) 439, 478, 1646, 1897
PinAI ACCGGT 1 cut(s) 1194
PleI GAGTC 5 cut(s) 295, 862, 1012, 2134, 2413
PpsI GAGTC 5 cut(s) 295, 862, 1012, 2134, 2413
PshAI GACNNNNGTC 1 cut(s) 1688
PsiI TTATAA 1 cut(s) 1781
Psp124BI GAGCTC 1 cut(s) 727
Psp6I CCWGG 2 cut(s) 821, 1621
PspFI CCCAGC 1 cut(s) 1640
PspGI CCWGG 2 cut(s) 821, 1621
PspN4I GGNNCC 4 cut(s) 214, 528, 1575, 1739
PspXI VCTCGAGB 1 cut(s) 726
PstNI CAGNNNCTG 1 cut(s) 2129
RsaI GTAC 6 cut(s) 340, 559, 828, 1142, 1923, 2198
RsaNI GTAC 6 cut(s) 339, 558, 827, 1141, 1922, 2197
RseI CAYNNNNRTG 3 cut(s) 156, 624, 893
SacI GAGCTC 1 cut(s) 727
SacII CCGCGG 1 cut(s) 2391
SaqAI TTAA 5 cut(s) 633, 690, 1032, 1847, 2210
Sau3AI GATC 4 cut(s) 170, 1186, 1789, 2142
ScaI AGTACT 1 cut(s) 1923
SchI GAGTC 5 cut(s) 296, 862, 1013, 2135, 2414
ScrFI CCNGG 4 cut(s) 531, 823, 1013, 1623
SduI GDGCHC 3 cut(s) 90, 531, 727
SexAI ACCWGGT 1 cut(s) 1621
SfaNI GCATC 4 cut(s) 540, 883, 930, 1520
Sfr274I CTCGAG 1 cut(s) 726
Sfr303I CCGCGG 1 cut(s) 2391
SgrBI CCGCGG 1 cut(s) 2391
SlaI CTCGAG 1 cut(s) 726
SmiMI CAYNNNNRTG 3 cut(s) 156, 624, 893
SmlI CTYRAG 3 cut(s) 322, 726, 1578
SmoI CTYRAG 3 cut(s) 322, 726, 1578
SpeI ACTAGT 1 cut(s) 2291
SphI GCATGC 1 cut(s) 857
SsiI CCGC 9 cut(s) 111, 245, 749, 783, 798, 947, 1272, 2388, 2390
SspI AATATT 1 cut(s) 2170
SspMI CTAG 4 cut(s) 234, 555, 2292, 2510
SstI GAGCTC 1 cut(s) 727
StyD4I CCNGG 4 cut(s) 529, 821, 1011, 1621
TaaI ACNGT 8 cut(s) 336, 1335, 1379, 1680, 1777, 1832, 1889, 2201
TaiI ACGT 2 cut(s) 455, 2504
TaqI TCGA 6 cut(s) 99, 727, 1036, 2078, 2098, 2459
TatI WGTACW 3 cut(s) 557, 1921, 2196
TauI GCSGC 3 cut(s) 801, 1275, 2393
TfiI GAWTC 4 cut(s) 439, 478, 1646, 1897
Tru1I TTAA 5 cut(s) 633, 690, 1032, 1847, 2210
Tru9I TTAA 5 cut(s) 633, 690, 1032, 1847, 2210
TscAI CASTG 7 cut(s) 341, 838, 1468, 1756, 1799, 1837, 2044
TseFI GTSAC 4 cut(s) 1310, 1379, 1841, 2135
Tsp45I GTSAC 4 cut(s) 1310, 1379, 1841, 2135
TspDTI ATGAA 9 cut(s) 420, 573, 608, 978, 1014, 1725, 1782, 1889, 2325
TspGWI ACGGA 5 cut(s) 279, 284, 1116, 1840, 2022
TspRI CASTG 7 cut(s) 341, 838, 1468, 1756, 1799, 1837, 2044
XapI RAATTY 4 cut(s) 304, 411, 1654, 1915
XceI RCATGY 2 cut(s) 857, 2197
XhoI CTCGAG 1 cut(s) 726
XmiI GTMKAC 1 cut(s) 704
XmnI GAANNNNTTC 2 cut(s) 1805, 2170
XspI CTAG 4 cut(s) 234, 555, 2292, 2510
ZraI GACGTC 1 cut(s) 2502
ZrmI AGTACT 1 cut(s) 1923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.