Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0015205

Physical Location & Seq

Genomic Coordinates Forward (+)

15793303 .. 15795630

Gene Structure 2328 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g13320.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGGCGTTCTCCTTCTCTCTCAAATGCTATCCATGGTCTCCCACCCACACCATACCCCAAACCCTGCCACCGCCGCCCTCCTCCGCTCAAAAACCCTTCACTTCTCTCTCCTTCCCGCTCTCTCACCGCCACGACCGGCTCCTCATCCATTCTTCCCTCTCCTATTCAACCCCTACCTCCACCCACCACCACCACCACCTCCCTCCGGACTTCACTCCGCAGCAGCTCCTCGATACCCTCCGCCGCCAGAACGACGAGTCCTCTGCGCTCCGCCTCTTCGACTGGGCCTCCAAGCAGCCCAATTTCACCCCCGACTCCACCGTGTACGAGGAGGTCCTCCGCAAGCTCGGAAAGGTGGGGTCTTTCGAGTCGATGAGGAGCATCCTTAACGAGATGAAGCACGTCGGCTGCCCTATTAGCTCAGGTACTTTCTTGATTTTTGTTCAGAGCTACGCAGCCTTTGATTTGTATGATGAAATTCTTGGGGTTGTGGAAATGATGGAAGGCGTGTTCGGGTGCAAACCCGACACTCATTTTTACAATTTCTTGTTGAATGTTGTTGTTGAGGGGAACAAACTTAAGCTTGTCGAAACTGCGAATTCGGGCATGCTTGGTAGAGGAATTAAGCCGGATGTTTCCACATTTAACATTTTGATAAAGGCATTGTGTAGAGCTCATCAGATTAGGCCTGCCCTTTTGTTGATGGAGGATATGTCGAACCATGGTTTGAAGCCGGATGAGAAAACGTTCACGACAGTAATGCAGGGGTACATTGAGGAAGGTGACATGAAAGGGGCATTGAGAATGAGGGACCAGATGGTAGAACATGGGTGCCCGTGTACAAATGTGACTGTTAATGTTTTGGTTAATGGGTTTTGCAAAGAGGGTCAGGTCGATGAAGCGTTCAGTTTTATAGAAAAGATGGTGGATGAGGGGTTCTCTCCGGATCAGTTTACGTTTAATACGTTGGTGAAGGGTTTGTGCAGGGTGGGGCATGTTACGCATGCTTTGGAGATCATGGACGTGATGCTTCAAGAGGGATTTGAACTGGATATTTACACGTATAACTCCTTGATTTCCGGATTGTGTAAGTTGGGTGAAATTGAAGAGGCAGTGGAACTTCTTGATCAAATGGTTTCGAGGGACTGTTCTCCAAATACTGTCACATATAATACTCTGATTAGCACATTGTGTAAGGAGAACCGGGTTGAAGAGGCAACCAAACTTGCTCGTGTTCTTACTAGCAAGGGAATTTTGCCTGATGTTTGTACTGTCAATTCTCTGATTCAAGGTCTCTGCTTGAACAGTAATCATAAAGCTGCTTTGGAACTATTTGAGGAGATGAAGGCATCAGGATGCCAGCCTGATGGGTTTACTTACAGTATGTTGATTGATAGCCACTGTTCGAGAGGGAGGCTAAAGGAGGCAATGAGCCTGCTGACAGAAATGGAGTTGAGAGGTTGTGCACGTAATGTGGTTATATACAATACTCTCATTGATGGGTTGTGCAAAAACAAAAGAATTGATGACGCAGAAGAGATTTTTGATCAGATGGAACTGCAGGGGATTTCAAGAAATTCAGTGACCTACAACACCCTTATTGATGGCCTTTGTCAGAGTAAACGTGTGGAGGAAGCTTCCCAGCTTATGGACCAGATGATAATGGAAGGCTTGAAGCCCGACAAGTTCACCTATAATTCCATGCTCACGTATTTCTGTAGAGCTGGGGATATAAAGAAGGCGGCAGATATTGTTCAGACAATGAGTTCAAATGGGTGTGAACCAGACATAGTCACATACGGGACATTGATTGGGGGGTTATGTAGGGCAGGTAGGGTTCAGGTTGCAAGCAGACTCCTCAGAAGTCTGCAGATGAAAGGGTTGATCCCATCTCCCCAGGCTTATAACCCCGTACTACAATCGCTATTTAAACGGAAGAGAACAACAGAAGCCATGAGGCTTTTCCGAGAGATGATGGAGAAAGGTGATCCTCCTGATTCTATAACATATAAGATTGTCCTACGCGGGCTTTGTAATGGTGGAGGACCAATTGGAGAAGCAGTTGACTTTGCAGTTGAGATGATGGAGAAAGGTTATTTACCAGAATTCTCATCATTTTCTATGCTGGCTGAAGGACTCCAAGCTTTATCCATGGAGGATACCTTAATTAACCTTGTTGATATGGTCATGGAGAAAGCAAAGCTTTCAGACAGTGAAGTTTCCATGATAAGCGGTTTCCTCAAAATCCGCAAATATCAGGATGCATTGGCCAACCTTGGTGGTATCTTGAATAGAGAAAAGCCCAGAAAATCTTATTGGCAAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016070 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

776

Amino Acids

86.81

Weight (kDa)

5.73

Isoelectric Point (pI)

35.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	175 - 224	5.7e-08	PPR repeat family
PPR_long	PF17177	176 - 322	1.1e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	206 - 238	3.6e-08	PPR repeat
PPR_2	PF13041	210 - 257	4.8e-13	PPR repeat family
PPR_2	PF13041	249 - 294	8.1e-12	PPR repeat family
TPR_24	PF23276	249 - 339	9.1e-07	Fungal tetratrico peptide repeats
TPR_24	PF23276	276 - 374	6.5e-08	Fungal tetratrico peptide repeats
PPR_1	PF12854	277 - 309	7.2e-10	PPR repeat
PPR	PF01535	283 - 313	1.3e-07	PPR repeat
PPR_2	PF13041	283 - 329	3.7e-16	PPR repeat family
PPR_1	PF12854	312 - 343	6.9e-10	PPR repeat
PPR_2	PF13041	318 - 364	5.7e-14	PPR repeat family
PPR_long	PF17177	328 - 477	2.8e-10	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	329 - 447	4.4e-10	Fungal tetratrico peptide repeats
PPR_3	PF13812	339 - 397	3.6e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	347 - 379	4.7e-13	PPR repeat
PPR_2	PF13041	351 - 399	7.8e-19	PPR repeat family
PPR	PF01535	353 - 383	6.2e-10	PPR repeat
PPR_1	PF12854	381 - 409	1.1e-11	PPR repeat
PPR_2	PF13041	385 - 433	8.2e-15	PPR repeat family
PPR_3	PF13812	413 - 465	2.9e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	416 - 449	5.6e-09	PPR repeat
PPR_2	PF13041	420 - 468	2.2e-16	PPR repeat family
PPR	PF01535	424 - 453	1.2e-06	PPR repeat
PPR_1	PF12854	452 - 484	1.1e-11	PPR repeat
PPR	PF01535	458 - 488	1.2e-06	PPR repeat
PPR_long	PF17177	467 - 605	1.8e-11	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	478 - 535	2.8e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	486 - 519	2.6e-14	PPR repeat
PPR_2	PF13041	490 - 539	3.6e-19	PPR repeat family
PPR	PF01535	493 - 523	1.1e-08	PPR repeat
PPR_1	PF12854	522 - 553	2e-12	PPR repeat
PPR_2	PF13041	525 - 569	7.1e-16	PPR repeat family
PPR	PF01535	528 - 558	3.7e-08	PPR repeat
PPR_3	PF13812	549 - 605	2.5e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	557 - 588	3e-08	PPR repeat
TPR_24	PF23276	561 - 658	7.1e-07	Fungal tetratrico peptide repeats
PPR_2	PF13041	563 - 609	3e-15	PPR repeat family
PPR_1	PF12854	591 - 621	7e-12	PPR repeat
PPR_2	PF13041	595 - 636	1.1e-06	PPR repeat family
PPR_2	PF13041	634 - 679	3.6e-13	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015205)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53700
fragaria_vesca	FvH4_6g14250
malus_domestica	MD04G1122700.v1.1 MD12G1138900.v1.1
prunus_persica	Prupe.6G250800_v2.0.a1
pyrus_communis	pycom12g13320
rosa_chinensis	RchiOBHm_Chr3g0467211
rosa_laevigata	RLG00000024519
rosa_multiflora	Rmu_sc0000962.1_g000073
rosa_roxburghii	Rroxscaffold_6G00413820
rosa_rugosa	Rorug03G0085700
rosa_samantha	Rh3AG134200 Rh3BG155500 Rh3CG155100 Rh3DG156300
rosa_wichuraiana	Rw3G012700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1905
Acc36I	ACCTGC	1 cut(s)	1820
AccB1I	GGYRCC	1 cut(s)	831
AccB7I	CCANNNNNTGG	1 cut(s)	1648
AccBSI	CCGCTC	2 cut(s)	86, 118
AccII	CGCG	1 cut(s)	2025
AccIII	TCCGGA	3 cut(s)	205, 943, 1079
AclI	AACGTT	1 cut(s)	746
AclWI	GGATC	3 cut(s)	954, 1879, 1982
AcoI	YGGCCR	1 cut(s)	2268
AcsI	RAATTY	5 cut(s)	477, 598, 1251, 1576, 2105
AcuI	CTGAAG	1 cut(s)	2151
AdeI	CACNNNGTG	1 cut(s)	1191
AfaI	GTAC	6 cut(s)	326, 427, 770, 841, 1270, 1914
AfiI	CCNNNNNNNGG	2 cut(s)	205, 1648
AflII	CTTAAG	1 cut(s)	578
AflIII	ACRYGT	2 cut(s)	1059, 1624
AjiI	CACGTC	2 cut(s)	403, 1024
AjnI	CCWGG	1 cut(s)	1896
AjuI	GAANNNNNNNTTGG	1 cut(s)	2299
AloI	GAACNNNNNNTCC	6 cut(s)	242, 274, 494, 526, 920, 952
Alw21I	GWGCWC	2 cut(s)	676, 1468
Alw26I	GTCTC	2 cut(s)	42, 1298
Alw44I	GTGCAC	1 cut(s)	1464
AlwI	GGATC	3 cut(s)	954, 1879, 1982
Aor13HI	TCCGGA	3 cut(s)	205, 943, 1079
AoxI	GGCC	4 cut(s)	285, 686, 1606, 2268
ApaLI	GTGCAC	1 cut(s)	1464
ApeKI	GCWGC	6 cut(s)	220, 223, 295, 408, 455, 1319
ApoI	RAATTY	5 cut(s)	477, 598, 1251, 1576, 2105
Asp700I	GAANNNNTTC	1 cut(s)	746
AspLEI	GCGC	1 cut(s)	268
AspS9I	GGNCC	5 cut(s)	285, 334, 811, 1651, 2045
AsuC2I	CCSGG	1 cut(s)	1205
AsuHPI	GGTGA	7 cut(s)	116, 298, 794, 982, 1109, 1681, 1997
AvaII	GGWCC	4 cut(s)	334, 811, 1651, 2045
BaeGI	GKGCMC	2 cut(s)	836, 1468
BalI	TGGCCA	1 cut(s)	2270
BanI	GGYRCC	1 cut(s)	831
BanII	GRGCYC	1 cut(s)	676
BauI	CACGAG	1 cut(s)	1230
Bbv12I	GWGCWC	2 cut(s)	676, 1468
BbvI	GCAGC	6 cut(s)	232, 235, 307, 395, 467, 1306
BcgI	CGANNNNNNTGC	4 cut(s)	245, 279, 742, 776
BciT130I	CCWGG	1 cut(s)	1898
BciVI	GTATCC	1 cut(s)	2152
BclI	TGATCA	2 cut(s)	1126, 1546
BcnI	CCSGG	1 cut(s)	1205
BcoDI	GTCTC	2 cut(s)	42, 1298
BfaI	CTAG	1 cut(s)	1242
BfmI	CTRYAG	3 cut(s)	1559, 1717, 1868
BfrI	CTTAAG	1 cut(s)	578
BfuAI	ACCTGC	1 cut(s)	1820
BfuI	GTATCC	1 cut(s)	2152
BisI	GCNGC	9 cut(s)	74, 221, 224, 244, 296, 409, 456, 1320, 1743
BlsI	GCNGC	9 cut(s)	75, 222, 225, 245, 297, 410, 457, 1321, 1744
Bme1390I	CCNGG	2 cut(s)	1205, 1898
Bme18I	GGWCC	4 cut(s)	334, 811, 1651, 2045
BmgBI	CACGTC	2 cut(s)	403, 1024
BmgT120I	GGNCC	5 cut(s)	285, 334, 811, 1651, 2045
BmiI	GGNNCC	3 cut(s)	140, 812, 833
BmrFI	CCNGG	2 cut(s)	1205, 1898
BmrI	ACTGGG	1 cut(s)	292
BmsI	GCATC	5 cut(s)	390, 1017, 1346, 1358, 2251
BmuI	ACTGGG	1 cut(s)	292
BplI	GAGNNNNNCTC	2 cut(s)	923, 955
Bpu10I	CCTNAGC	1 cut(s)	421
BpuMI	CCSGG	1 cut(s)	1205
BsaAI	YACGTR	3 cut(s)	1062, 1469, 1710
BsaI	GGTCTC	2 cut(s)	42, 1298
BsaJI	CCNNGG	5 cut(s)	32, 721, 1896, 2151, 2275
BsaWI	WCCGGW	3 cut(s)	205, 943, 1079
BsaXI	ACNNNNNCTCC	6 cut(s)	272, 302, 698, 728, 2046, 2076
Bsc4I	CCNNNNNNNGG	2 cut(s)	205, 1648
Bse118I	RCCGGY	1 cut(s)	135
Bse1I	ACTGG	2 cut(s)	287, 1053
Bse3DI	GCAATG	1 cut(s)	1434
BseAI	TCCGGA	3 cut(s)	205, 943, 1079
BseBI	CCWGG	1 cut(s)	1898
BseDI	CCNNGG	5 cut(s)	32, 721, 1896, 2151, 2275
BseGI	GGATG	7 cut(s)	144, 381, 637, 742, 934, 1361, 2266
BseLI	CCNNNNNNNGG	2 cut(s)	205, 1648
BseMI	GCAATG	1 cut(s)	1434
BseMII	CTCAG	2 cut(s)	435, 1873
BseNI	ACTGG	2 cut(s)	287, 1053
BseRI	GAGGAG	7 cut(s)	70, 131, 218, 344, 391, 1352, 1847
BseSI	GKGCMC	2 cut(s)	836, 1468
BseXI	GCAGC	6 cut(s)	232, 235, 307, 395, 467, 1306
BseYI	CCCAGC	2 cut(s)	1641, 1724
BsgI	GTGCAG	1 cut(s)	1003
Bsh1236I	CGCG	1 cut(s)	2025
Bsh1285I	CGRYCG	1 cut(s)	136
BshFI	GGCC	4 cut(s)	287, 688, 1608, 2270
BshNI	GGYRCC	1 cut(s)	831
BsiEI	CGRYCG	1 cut(s)	136
BsiHKAI	GWGCWC	2 cut(s)	676, 1468
BsiSI	CCGG	7 cut(s)	136, 206, 629, 734, 944, 1080, 1204
BslFI	GGGAC	3 cut(s)	824, 1157, 1816
BslI	CCNNNNNNNGG	2 cut(s)	205, 1648
BsmAI	GTCTC	2 cut(s)	42, 1298
BsmFI	GGGAC	3 cut(s)	824, 1157, 1816
BsnI	GGCC	4 cut(s)	287, 688, 1608, 2270
Bso31I	GGTCTC	2 cut(s)	42, 1298
Bsp1286I	GDGCHC	3 cut(s)	676, 836, 1468
Bsp13I	TCCGGA	3 cut(s)	205, 943, 1079
Bsp1407I	TGTACA	1 cut(s)	839
Bsp143I	GATC	6 cut(s)	946, 1014, 1126, 1546, 1884, 1987
Bsp19I	CCATGG	3 cut(s)	32, 721, 2151
BspANI	GGCC	4 cut(s)	287, 688, 1608, 2270
BspCNI	CTCAG	2 cut(s)	434, 1872
BspEI	TCCGGA	3 cut(s)	205, 943, 1079
BspFNI	CGCG	1 cut(s)	2025
BspLI	GGNNCC	3 cut(s)	140, 812, 833
BspMAI	CTGCAG	2 cut(s)	1563, 1872
BspMI	ACCTGC	1 cut(s)	1820
BspPI	GGATC	3 cut(s)	954, 1879, 1982
BspT107I	GGYRCC	1 cut(s)	831
BspTI	CTTAAG	1 cut(s)	578
BspTNI	GGTCTC	2 cut(s)	42, 1298
BsrBI	CCGCTC	2 cut(s)	86, 118
BsrDI	GCAATG	1 cut(s)	1434
BsrFI	RCCGGY	1 cut(s)	135
BsrGI	TGTACA	1 cut(s)	839
BsrI	ACTGG	2 cut(s)	287, 1053
BssAI	RCCGGY	1 cut(s)	135
BssECI	CCNNGG	5 cut(s)	32, 721, 1896, 2151, 2275
BssMI	GATC	6 cut(s)	946, 1014, 1126, 1546, 1884, 1987
BssSI	CACGAG	1 cut(s)	1230
BssT1I	CCWWGG	4 cut(s)	32, 721, 2151, 2275
Bst2BI	CACGAG	1 cut(s)	1230
Bst2UI	CCWGG	1 cut(s)	1898
Bst6I	CTCTTC	5 cut(s)	281, 1101, 1206, 1530, 1931
BstAFI	CTTAAG	1 cut(s)	578
BstAUI	TGTACA	1 cut(s)	839
BstBAI	YACGTR	3 cut(s)	1062, 1469, 1710
BstC8I	GCNNGC	9 cut(s)	344, 608, 690, 1005, 1361, 1436, 1849, 2027, 2127
BstDEI	CTNAG	2 cut(s)	421, 1859
BstDSI	CCRYGG	3 cut(s)	32, 721, 2151
BstF5I	GGATG	7 cut(s)	144, 381, 637, 742, 934, 1361, 2266
BstFNI	CGCG	1 cut(s)	2025
BstHHI	GCGC	1 cut(s)	268
BstKTI	GATC	6 cut(s)	949, 1017, 1129, 1549, 1887, 1990
BstMAI	GTCTC	2 cut(s)	42, 1298
BstMBI	GATC	6 cut(s)	946, 1014, 1126, 1546, 1884, 1987
BstMCI	CGRYCG	1 cut(s)	136
BstMWI	GCNNNNNNNGC	3 cut(s)	73, 417, 1000
BstNI	CCWGG	1 cut(s)	1898
BstNSI	RCATGY	3 cut(s)	610, 998, 1007
BstSCI	CCNGG	2 cut(s)	1203, 1896
BstSFI	CTRYAG	3 cut(s)	1559, 1717, 1868
BstSLI	GKGCMC	2 cut(s)	836, 1468
BstUI	CGCG	1 cut(s)	2025
BstV1I	GCAGC	6 cut(s)	232, 235, 307, 395, 467, 1306
BstXI	CCANNNNNNTGG	1 cut(s)	1367
BsuI	GTATCC	1 cut(s)	2152
BsuRI	GGCC	4 cut(s)	287, 688, 1608, 2270
BtgI	CCRYGG	3 cut(s)	32, 721, 2151
BtrI	CACGTC	2 cut(s)	403, 1024
BtsCI	GGATG	7 cut(s)	144, 381, 637, 742, 934, 1361, 2266
BtsI	GCAGTG	1 cut(s)	1119
BtsIMutI	CAGTG	4 cut(s)	1119, 1399, 1587, 2218
BveI	ACCTGC	1 cut(s)	1820
Cac8I	GCNNGC	9 cut(s)	344, 608, 690, 1005, 1361, 1436, 1849, 2027, 2127
CfoI	GCGC	1 cut(s)	268
Cfr10I	RCCGGY	1 cut(s)	135
Cfr13I	GGNCC	5 cut(s)	285, 334, 811, 1651, 2045
CseI	GACGC	1 cut(s)	1538
Csp6I	GTAC	6 cut(s)	325, 426, 769, 840, 1269, 1913
CspCI	CAANNNNNGTGG	2 cut(s)	2260, 2295
CviQI	GTAC	6 cut(s)	325, 426, 769, 840, 1269, 1913
DdeI	CTNAG	2 cut(s)	421, 1859
DpnI	GATC	6 cut(s)	948, 1016, 1128, 1548, 1886, 1989
DpnII	GATC	6 cut(s)	946, 1014, 1126, 1546, 1884, 1987
DraI	TTTAAA	1 cut(s)	1930
DraIII	CACNNNGTG	1 cut(s)	1191
EaeI	YGGCCR	1 cut(s)	2268
Eam1104I	CTCTTC	5 cut(s)	281, 1101, 1206, 1530, 1931
EarI	CTCTTC	5 cut(s)	281, 1101, 1206, 1530, 1931
EciI	GGCGGA	2 cut(s)	230, 260
Ecl136II	GAGCTC	1 cut(s)	674
Eco130I	CCWWGG	4 cut(s)	32, 721, 2151, 2275
Eco147I	AGGCCT	1 cut(s)	688
Eco24I	GRGCYC	1 cut(s)	676
Eco31I	GGTCTC	2 cut(s)	42, 1298
Eco47I	GGWCC	4 cut(s)	334, 811, 1651, 2045
Eco53kI	GAGCTC	1 cut(s)	674
Eco57I	CTGAAG	1 cut(s)	2151
EcoICRI	GAGCTC	1 cut(s)	674
EcoO109I	RGGNCCY	1 cut(s)	334
EcoRI	GAATTC	2 cut(s)	598, 2105
EcoRII	CCWGG	1 cut(s)	1896
EcoT14I	CCWWGG	4 cut(s)	32, 721, 2151, 2275
EcoT22I	ATGCAT	1 cut(s)	2266
EcoT38I	GRGCYC	1 cut(s)	676
ErhI	CCWWGG	4 cut(s)	32, 721, 2151, 2275
FalI	AAGNNNNNCTT	2 cut(s)	2187, 2219
FaqI	GGGAC	3 cut(s)	824, 1157, 1816
FauI	CCCGC	2 cut(s)	123, 2018
FbaI	TGATCA	2 cut(s)	1126, 1546
Fnu4HI	GCNGC	9 cut(s)	74, 221, 224, 244, 296, 409, 456, 1320, 1743
FokI	GGATG	7 cut(s)	131, 368, 644, 749, 941, 1368, 2273
FriOI	GRGCYC	1 cut(s)	676
Fsp4HI	GCNGC	9 cut(s)	74, 221, 224, 244, 296, 409, 456, 1320, 1743
FspBI	CTAG	1 cut(s)	1242
GlaI	GCGC	1 cut(s)	267
GluI	GCNGC	9 cut(s)	74, 221, 224, 244, 296, 409, 456, 1320, 1743
GsaI	CCCAGC	2 cut(s)	1645, 1728
HaeIII	GGCC	4 cut(s)	287, 688, 1608, 2270
HapII	CCGG	7 cut(s)	136, 206, 629, 734, 944, 1080, 1204
HgaI	GACGC	1 cut(s)	1538
HhaI	GCGC	1 cut(s)	268
Hin6I	GCGC	1 cut(s)	266
HinP1I	GCGC	1 cut(s)	266
HincII	GTYRAC	1 cut(s)	2065
HindII	GTYRAC	1 cut(s)	2065
HindIII	AAGCTT	4 cut(s)	581, 1635, 2142, 2201
HinfI	GANTC	7 cut(s)	257, 314, 368, 1285, 1854, 1997, 2136
HpaII	CCGG	7 cut(s)	136, 206, 629, 734, 944, 1080, 1204
HphI	GGTGA	7 cut(s)	116, 298, 794, 982, 1109, 1681, 1997
Hpy99I	CGWCG	2 cut(s)	257, 407
HpyCH4IV	ACGT	9 cut(s)	402, 746, 956, 965, 1023, 1061, 1468, 1624, 1709
HpyF10VI	GCNNNNNNNGC	3 cut(s)	73, 417, 1000
HpyF3I	CTNAG	2 cut(s)	421, 1859
HpySE526I	ACGT	9 cut(s)	402, 746, 956, 965, 1023, 1061, 1468, 1624, 1709
HspAI	GCGC	1 cut(s)	266
Kpn2I	TCCGGA	3 cut(s)	205, 943, 1079
Ksp22I	TGATCA	2 cut(s)	1126, 1546
Kzo9I	GATC	6 cut(s)	946, 1014, 1126, 1546, 1884, 1987
LmnI	GCTCC	4 cut(s)	144, 231, 273, 378
Lsp1109I	GCAGC	6 cut(s)	232, 235, 307, 395, 467, 1306
LweI	GCATC	5 cut(s)	390, 1017, 1346, 1358, 2251
MaeI	CTAG	1 cut(s)	1242
MaeII	ACGT	9 cut(s)	402, 746, 956, 965, 1023, 1061, 1468, 1624, 1709
MaeIII	GTNAC	6 cut(s)	782, 847, 997, 1162, 1582, 1792
MalI	GATC	6 cut(s)	948, 1016, 1128, 1548, 1886, 1989
MbiI	CCGCTC	2 cut(s)	86, 118
MboI	GATC	6 cut(s)	946, 1014, 1126, 1546, 1884, 1987
MboII	GAAGA	6 cut(s)	144, 268, 1118, 1223, 1547, 1948
MfeI	CAATTG	1 cut(s)	2049
MhlI	GDGCHC	3 cut(s)	676, 836, 1468
MlsI	TGGCCA	1 cut(s)	2270
MluNI	TGGCCA	1 cut(s)	2270
MlyI	GAGTC	5 cut(s)	266, 308, 377, 1848, 2130
Mox20I	TGGCCA	1 cut(s)	2270
Mph1103I	ATGCAT	1 cut(s)	2266
MroI	TCCGGA	3 cut(s)	205, 943, 1079
MroXI	GAANNNNTTC	1 cut(s)	746
MscI	TGGCCA	1 cut(s)	2270
MslI	CAYNNNNRTG	2 cut(s)	1022, 1354
Msp20I	TGGCCA	1 cut(s)	2270
MspCI	CTTAAG	1 cut(s)	578
MspI	CCGG	7 cut(s)	136, 206, 629, 734, 944, 1080, 1204
MspR9I	CCNGG	2 cut(s)	1205, 1898
MunI	CAATTG	1 cut(s)	2049
MvaI	CCWGG	1 cut(s)	1898
MvnI	CGCG	1 cut(s)	2025
MwoI	GCNNNNNNNGC	3 cut(s)	73, 417, 1000
NciI	CCSGG	1 cut(s)	1205
NcoI	CCATGG	3 cut(s)	32, 721, 2151
NdeII	GATC	6 cut(s)	946, 1014, 1126, 1546, 1884, 1987
NlaIV	GGNNCC	3 cut(s)	140, 812, 833
NmuCI	GTSAC	5 cut(s)	782, 847, 1162, 1582, 1792
NsiI	ATGCAT	1 cut(s)	2266
NspI	RCATGY	3 cut(s)	610, 998, 1007
PacI	TTAATTAA	1 cut(s)	2169
PaeI	GCATGC	2 cut(s)	610, 1007
PceI	AGGCCT	1 cut(s)	688
PcsI	WCGNNNNNNNCGW	1 cut(s)	962
PdmI	GAANNNNTTC	1 cut(s)	746
PfeI	GAWTC	2 cut(s)	1285, 1997
PflFI	GACNNNGTC	1 cut(s)	1790
PflMI	CCANNNNNTGG	1 cut(s)	1648
PkrI	GCNGC	9 cut(s)	75, 222, 225, 245, 297, 410, 457, 1321, 1744
PleI	GAGTC	5 cut(s)	265, 308, 376, 1848, 2130
PpsI	GAGTC	5 cut(s)	265, 308, 376, 1848, 2130
Ppu21I	YACGTR	3 cut(s)	1062, 1469, 1710
PpuMI	RGGWCCY	1 cut(s)	334
PsiI	TTATAA	1 cut(s)	1905
Psp124BI	GAGCTC	1 cut(s)	676
Psp1406I	AACGTT	1 cut(s)	746
Psp5II	RGGWCCY	1 cut(s)	334
Psp6I	CCWGG	1 cut(s)	1896
PspFI	CCCAGC	2 cut(s)	1641, 1724
PspGI	CCWGG	1 cut(s)	1896
PspN4I	GGNNCC	3 cut(s)	140, 812, 833
PspPI	GGNCC	5 cut(s)	285, 334, 811, 1651, 2045
PspPPI	RGGWCCY	1 cut(s)	334
PstI	CTGCAG	2 cut(s)	1563, 1872
PsyI	GACNNNGTC	1 cut(s)	1790
RsaI	GTAC	6 cut(s)	326, 427, 770, 841, 1270, 1914
RsaNI	GTAC	6 cut(s)	325, 426, 769, 840, 1269, 1913
RseI	CAYNNNNRTG	2 cut(s)	1022, 1354
SacI	GAGCTC	1 cut(s)	676
SatI	GCNGC	9 cut(s)	74, 221, 224, 244, 296, 409, 456, 1320, 1743
Sau3AI	GATC	6 cut(s)	946, 1014, 1126, 1546, 1884, 1987
Sau96I	GGNCC	5 cut(s)	285, 334, 811, 1651, 2045
SchI	GAGTC	5 cut(s)	266, 308, 377, 1848, 2130
ScrFI	CCNGG	2 cut(s)	1205, 1898
SduI	GDGCHC	3 cut(s)	676, 836, 1468
SfaNI	GCATC	5 cut(s)	390, 1017, 1346, 1358, 2251
SfcI	CTRYAG	3 cut(s)	1559, 1717, 1868
SinI	GGWCC	4 cut(s)	334, 811, 1651, 2045
SmiMI	CAYNNNNRTG	2 cut(s)	1022, 1354
SmlI	CTYRAG	1 cut(s)	578
SmoI	CTYRAG	1 cut(s)	578
SphI	GCATGC	2 cut(s)	610, 1007
SseBI	AGGCCT	1 cut(s)	688
SspMI	CTAG	1 cut(s)	1242
SstI	GAGCTC	1 cut(s)	676
StuI	AGGCCT	1 cut(s)	688
StyD4I	CCNGG	2 cut(s)	1203, 1896
StyI	CCWWGG	4 cut(s)	32, 721, 2151, 2275
TaiI	ACGT	9 cut(s)	405, 749, 959, 968, 1026, 1064, 1471, 1627, 1712
TaqI	TCGA	9 cut(s)	231, 279, 366, 371, 588, 716, 894, 1139, 1406
TatI	WGTACW	2 cut(s)	839, 1268
TauI	GCSGC	3 cut(s)	76, 246, 1745
TfiI	GAWTC	2 cut(s)	1285, 1997
TscAI	CASTG	4 cut(s)	1119, 1406, 1587, 2218
TseFI	GTSAC	5 cut(s)	782, 847, 1162, 1582, 1792
TseI	GCWGC	6 cut(s)	220, 223, 295, 408, 455, 1319
Tsp45I	GTSAC	5 cut(s)	782, 847, 1162, 1582, 1792
TspDTI	ATGAA	6 cut(s)	410, 489, 803, 912, 1358, 1889
TspGWI	ACGGA	1 cut(s)	1948
TspRI	CASTG	4 cut(s)	1119, 1406, 1587, 2218
Tth111I	GACNNNGTC	1 cut(s)	1790
Van91I	CCANNNNNTGG	1 cut(s)	1648
Vha464I	CTTAAG	1 cut(s)	578
VneI	GTGCAC	1 cut(s)	1464
VpaK11BI	GGWCC	4 cut(s)	334, 811, 1651, 2045
XapI	RAATTY	5 cut(s)	477, 598, 1251, 1576, 2105
XceI	RCATGY	3 cut(s)	610, 998, 1007
XmnI	GAANNNNTTC	1 cut(s)	746
XspI	CTAG	1 cut(s)	1242
Zsp2I	ATGCAT	1 cut(s)	2266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.