pycom12g13840

protein At2g37660, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012954
Physical Location & Seq
Reverse (-)
16310233 .. 16313185
2953 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
ATGGCGGATTCGGGTGCAAGCACCGTCCTTGTCACTGGAGCTGGTGGTAGAACTGGTAAACTAGTTTATAAGAAGTTAAAGGGGAGGTCAGACCAGTATGTTGCTAGGGGTCTGGTTAGGACTGAAGAGAGCAAACAGAGCGTTGGCGGAGCAGATGATCTTTATGTTGGAGATATAAGGGATGCTGACAGCATTCTACCTGCAATCCAAGGCATCGATGCTCTCATAATCCTTACAAGCGCTGTGCCTAAGATGAAACCTGGCTTTGATCCGACCAAAGGTGGGAGGCCTGAGTTCTATTTTGAAGACGGGTCGTATCCTGAACAGGTTGATTGGATTGGACAGAAAAACCAAATAGATGCTGCTAAGGCTGCGGGAGTGAAGCAGATTGTGTTAGTTGGGTCTATGGGCGGAACAGATCTTAACAATCCCCTGAACAAATTGGCTAATGGCAACATATTGGTTTGGAAGAGGAAGGCTGAGCAGTATTTGGCTGACTCCGGAATTCCATACACAATCATAAGGGCTGGAGGCTTGCAAGACAAAGAAGGAGGCATCCGTGAACTACTTATAGGGAAGGATGACGAGCTTCTTAAGACCGAAACAAGAACCATCGCTAGACCTGATGTCGCAGAAGTCTGCGTTCAGGCACTGCAGTTCGAGGAGGCCAAGTTCAAGGCATTTGATTTGGCCTCAAAGCCGGAGGGAACTGGGACACCGACCAAGGATTTTAAGGCTTTATTTTCCCAGATCACTACCCGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAD_binding_10

Protein Analysis

256

Amino Acids

27.47

Weight (kDa)

6.44

Isoelectric Point (pI)

30.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 174 5.6e-08 NmrA-like family
NAD_binding_10 PF13460 13 - 219 1.5e-33 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012954)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37660 AT5G02240
fragaria_vesca FvH4_6g13500
malus_domestica MD04G1130500.v1.1 MD12G1143700.v1.1
prunus_persica Prupe.6G257200_v2.0.a1 Prupe.6G257200_v2.0.a1
pyrus_communis pycom04g11880 pycom12g13840
rosa_chinensis RchiOBHm_Chr3g0466371
rosa_laevigata RLG00000024580
rosa_multiflora Rmu_sc0003480.1_g000005
rosa_roxburghii Rroxscaffold_6G00414530
rosa_rugosa Rorug03G0081300
rosa_samantha Rh3AG127800 Rh3BG149100 Rh3DG149800
rosa_wichuraiana Rw3G012070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 69
Acc36I ACCTGC 1 cut(s) 208
AccIII TCCGGA 1 cut(s) 500
AciI CCGC 4 cut(s) 5, 147, 374, 411
AclWI GGATC 1 cut(s) 263
AcsI RAATTY 1 cut(s) 504
AcuI CTGAAG 1 cut(s) 144
AfeI AGCGCT 1 cut(s) 241
AfiI CCNNNNNNNGG 2 cut(s) 278, 282
AflII CTTAAG 1 cut(s) 593
AgsI TTSAA 2 cut(s) 305, 676
AhdI GACNNNNNGTC 1 cut(s) 626
AhlI ACTAGT 1 cut(s) 61
AjnI CCWGG 1 cut(s) 259
AloI GAACNNNNNNTCC 2 cut(s) 656, 688
AluBI AGCT 2 cut(s) 41, 589
AluI AGCT 2 cut(s) 41, 589
AlwI GGATC 1 cut(s) 263
AlwNI CAGNNNCTG 1 cut(s) 652
Aor13HI TCCGGA 1 cut(s) 500
Aor51HI AGCGCT 1 cut(s) 241
AoxI GGCC 3 cut(s) 287, 666, 690
ApeKI GCWGC 2 cut(s) 362, 371
ApoI RAATTY 1 cut(s) 504
AspLEI GCGC 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 312
BbvI GCAGC 2 cut(s) 349, 358
BccI CCATC 1 cut(s) 620
BciT130I CCWGG 1 cut(s) 261
BciVI GTATCC 1 cut(s) 327
BcuI ACTAGT 1 cut(s) 61
BfaI CTAG 3 cut(s) 62, 105, 618
BfmI CTRYAG 1 cut(s) 653
BfoI RGCGCY 1 cut(s) 243
BfrI CTTAAG 1 cut(s) 593
BfuAI ACCTGC 1 cut(s) 208
BfuI GTATCC 1 cut(s) 327
BglII AGATCT 1 cut(s) 418
BisI GCNGC 2 cut(s) 363, 372
BlpI GCTNAGC 1 cut(s) 480
BlsI GCNGC 2 cut(s) 364, 373
Bme1390I CCNGG 1 cut(s) 261
BmeRI GACNNNNNGTC 1 cut(s) 626
BmrFI CCNGG 1 cut(s) 261
BmrI ACTGGG 1 cut(s) 720
BmsI GCATC 5 cut(s) 172, 208, 222, 349, 564
BmuI ACTGGG 1 cut(s) 720
BpiI GAAGAC 1 cut(s) 312
BpmI CTGGAG 2 cut(s) 57, 549
Bpu10I CCTNAGC 1 cut(s) 366
Bpu1102I GCTNAGC 1 cut(s) 480
Bsa29I ATCGAT 1 cut(s) 216
BsaJI CCNNGG 2 cut(s) 208, 723
BsaWI WCCGGW 1 cut(s) 500
BsaXI ACNNNNNCTCC 2 cut(s) 656, 686
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 282
Bse1I ACTGG 4 cut(s) 40, 58, 94, 715
BseAI TCCGGA 1 cut(s) 500
BseBI CCWGG 1 cut(s) 261
BseCI ATCGAT 1 cut(s) 216
BseDI CCNNGG 2 cut(s) 208, 723
BseGI GGATG 3 cut(s) 187, 555, 586
BseLI CCNNNNNNNGG 2 cut(s) 278, 282
BseMII CTCAG 2 cut(s) 282, 471
BseNI ACTGG 4 cut(s) 40, 58, 94, 715
BseRI GAGGAG 1 cut(s) 677
BseXI GCAGC 2 cut(s) 349, 358
BshFI GGCC 3 cut(s) 289, 668, 692
BshVI ATCGAT 1 cut(s) 216
BsiSI CCGG 2 cut(s) 501, 701
BslFI GGGAC 1 cut(s) 727
BslI CCNNNNNNNGG 2 cut(s) 278, 282
BsmFI GGGAC 1 cut(s) 727
BsmI GAATGC 1 cut(s) 192
BsnI GGCC 3 cut(s) 289, 668, 692
Bsp13I TCCGGA 1 cut(s) 500
Bsp143I GATC 4 cut(s) 157, 268, 418, 750
Bsp1720I GCTNAGC 1 cut(s) 480
BspACI CCGC 4 cut(s) 5, 147, 374, 411
BspANI GGCC 3 cut(s) 289, 668, 692
BspCNI CTCAG 2 cut(s) 283, 472
BspDI ATCGAT 1 cut(s) 216
BspEI TCCGGA 1 cut(s) 500
BspMAI CTGCAG 1 cut(s) 657
BspMI ACCTGC 1 cut(s) 208
BspPI GGATC 1 cut(s) 263
BspTI CTTAAG 1 cut(s) 593
BsrI ACTGG 4 cut(s) 40, 58, 94, 715
BssECI CCNNGG 2 cut(s) 208, 723
BssMI GATC 4 cut(s) 157, 268, 418, 750
BssT1I CCWWGG 2 cut(s) 208, 723
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 2 cut(s) 120, 464
BstAFI CTTAAG 1 cut(s) 593
BstC8I GCNNGC 2 cut(s) 19, 536
BstDEI CTNAG 4 cut(s) 249, 291, 366, 480
BstF5I GGATG 3 cut(s) 187, 555, 586
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 4 cut(s) 160, 271, 421, 753
BstMBI GATC 4 cut(s) 157, 268, 418, 750
BstMWI GCNNNNNNNGC 3 cut(s) 138, 368, 371
BstNI CCWGG 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 259
BstSFI CTRYAG 1 cut(s) 653
BstV1I GCAGC 2 cut(s) 349, 358
BstV2I GAAGAC 1 cut(s) 312
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
Bsu15I ATCGAT 1 cut(s) 216
BsuI GTATCC 1 cut(s) 327
BsuRI GGCC 3 cut(s) 289, 668, 692
BsuTUI ATCGAT 1 cut(s) 216
BtgZI GCGATG 1 cut(s) 598
BtsCI GGATG 3 cut(s) 187, 555, 586
BtsI GCAGTG 1 cut(s) 650
BtsIMutI CAGTG 2 cut(s) 33, 650
BveI ACCTGC 1 cut(s) 208
Cac8I GCNNGC 2 cut(s) 19, 536
CaiI CAGNNNCTG 1 cut(s) 652
CfoI GCGC 1 cut(s) 242
ClaI ATCGAT 1 cut(s) 216
DdeI CTNAG 4 cut(s) 249, 291, 366, 480
DpnI GATC 4 cut(s) 159, 270, 420, 752
DpnII GATC 4 cut(s) 157, 268, 418, 750
DriI GACNNNNNGTC 1 cut(s) 626
Eam1104I CTCTTC 2 cut(s) 120, 464
Eam1105I GACNNNNNGTC 1 cut(s) 626
EarI CTCTTC 2 cut(s) 120, 464
EciI GGCGGA 3 cut(s) 20, 162, 426
Eco130I CCWWGG 2 cut(s) 208, 723
Eco147I AGGCCT 1 cut(s) 289
Eco47III AGCGCT 1 cut(s) 241
Eco57I CTGAAG 1 cut(s) 144
EcoRI GAATTC 1 cut(s) 504
EcoRII CCWGG 1 cut(s) 259
EcoT14I CCWWGG 2 cut(s) 208, 723
ErhI CCWWGG 2 cut(s) 208, 723
FaqI GGGAC 1 cut(s) 727
FauI CCCGC 1 cut(s) 367
Fnu4HI GCNGC 2 cut(s) 363, 372
FokI GGATG 3 cut(s) 194, 542, 593
Fsp4HI GCNGC 2 cut(s) 363, 372
FspBI CTAG 3 cut(s) 62, 105, 618
GlaI GCGC 1 cut(s) 241
GluI GCNGC 2 cut(s) 363, 372
GsuI CTGGAG 2 cut(s) 57, 549
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 3 cut(s) 289, 668, 692
HapII CCGG 2 cut(s) 501, 701
HhaI GCGC 1 cut(s) 242
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HinfI GANTC 2 cut(s) 8, 497
HpaII CCGG 2 cut(s) 501, 701
Hpy166II GTNNAC 2 cut(s) 59, 563
Hpy188I TCNGA 2 cut(s) 91, 273
Hpy188III TCNNGA 2 cut(s) 320, 501
Hpy8I GTNNAC 2 cut(s) 59, 563
HpyAV CCTTC 3 cut(s) 469, 542, 571
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4V TGCA 4 cut(s) 17, 203, 538, 655
HpyF10VI GCNNNNNNNGC 3 cut(s) 138, 368, 371
HpyF3I CTNAG 4 cut(s) 249, 291, 366, 480
HspAI GCGC 1 cut(s) 240
Kpn2I TCCGGA 1 cut(s) 500
Kzo9I GATC 4 cut(s) 157, 268, 418, 750
LmnI GCTCC 2 cut(s) 38, 149
Lsp1109I GCAGC 2 cut(s) 349, 358
LweI GCATC 5 cut(s) 172, 208, 222, 349, 564
MaeI CTAG 3 cut(s) 62, 105, 618
MaeIII GTNAC 1 cut(s) 31
MalI GATC 4 cut(s) 159, 270, 420, 752
MboI GATC 4 cut(s) 157, 268, 418, 750
MboII GAAGA 3 cut(s) 137, 317, 481
MflI RGATCY 1 cut(s) 418
MluCI AATT 2 cut(s) 440, 504
MlyI GAGTC 1 cut(s) 491
MmeI TCCRAC 2 cut(s) 148, 296
MnlI CCTC 9 cut(s) 78, 279, 465, 524, 545, 655, 658, 697, 703
MroI TCCGGA 1 cut(s) 500
MseI TTAA 4 cut(s) 77, 423, 594, 732
MspCI CTTAAG 1 cut(s) 593
MspI CCGG 2 cut(s) 501, 701
MspR9I CCNGG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 192
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 3 cut(s) 138, 368, 371
NdeII GATC 4 cut(s) 157, 268, 418, 750
NmuCI GTSAC 1 cut(s) 31
PceI AGGCCT 1 cut(s) 289
PctI GAATGC 1 cut(s) 192
PfeI GAWTC 1 cut(s) 8
PkrI GCNGC 2 cut(s) 364, 373
PleI GAGTC 1 cut(s) 491
PpsI GAGTC 1 cut(s) 491
PsiI TTATAA 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
PstI CTGCAG 1 cut(s) 657
PstNI CAGNNNCTG 1 cut(s) 652
PsuI RGATCY 1 cut(s) 418
SaqAI TTAA 4 cut(s) 77, 423, 594, 732
SatI GCNGC 2 cut(s) 363, 372
Sau3AI GATC 4 cut(s) 157, 268, 418, 750
SchI GAGTC 1 cut(s) 491
ScrFI CCNGG 1 cut(s) 261
SetI ASST 8 cut(s) 43, 89, 202, 262, 283, 330, 591, 625
SfaNI GCATC 5 cut(s) 172, 208, 222, 349, 564
SfcI CTRYAG 1 cut(s) 653
SmlI CTYRAG 1 cut(s) 593
SmoI CTYRAG 1 cut(s) 593
SpeI ACTAGT 1 cut(s) 61
Sse9I AATT 2 cut(s) 440, 504
SseBI AGGCCT 1 cut(s) 289
SsiI CCGC 4 cut(s) 5, 147, 374, 411
SspMI CTAG 3 cut(s) 62, 105, 618
StuI AGGCCT 1 cut(s) 289
StyD4I CCNGG 1 cut(s) 259
StyI CCWWGG 2 cut(s) 208, 723
TaaI ACNGT 1 cut(s) 25
TaqI TCGA 2 cut(s) 216, 660
TaqII GACCGA 1 cut(s) 614
TasI AATT 2 cut(s) 440, 504
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 4 cut(s) 77, 423, 594, 732
Tru9I TTAA 4 cut(s) 77, 423, 594, 732
TscAI CASTG 2 cut(s) 40, 657
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 2 cut(s) 362, 371
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 1 cut(s) 269
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 2 cut(s) 40, 657
Vha464I CTTAAG 1 cut(s) 593
XapI RAATTY 1 cut(s) 504
XspI CTAG 3 cut(s) 62, 105, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.