pycom12g15780

Belongs to the yippee family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007629
Physical Location & Seq
Reverse (-)
18225658 .. 18227821
2164 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 381 bp
ATGTGCGAAATGGGAAGGCTGTTTGTGATCACTCTCGAGGAAGACATGTATAGCTGCAAGCACTGCAGAACCCATCTCGCTCTTGTCGACGACGTAATCTCTAAGTCTTTTCACTCCAGGTATGGGAAGGCTTATCTTTTCGATAAAGTCGTGAATGTCACACTTGGAGAGAAAGAAGAGCGGATCATGATAACAGGATTGTATACCGTTGCTGACATATTTTGTGTTTCATGTGGATCAAATGTGGGATTGAAATATGAGGCGGCGCATGAGAAATCCCAGAAGTACAAGGAAGGAAAGTGCATCCTTGAGAGATTCCAGATTCTCGGTCCTGATGGAAGCGACTACTCAATTGCTCTCGAAGCTCAGATTGGCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yippee-Mis18

Protein Analysis

127

Amino Acids

14.16

Weight (kDa)

5.78

Isoelectric Point (pI)

19.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yippee-Mis18 PF03226 17 - 107 3.2e-13 Yippee zinc-binding/DNA-binding /Mis18, centromere assembly
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 181
AccI GTMKAC 2 cut(s) 87, 203
AciI CCGC 3 cut(s) 181, 263, 375
AclWI GGATC 2 cut(s) 191, 244
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 1 cut(s) 123
AflIII ACRYGT 1 cut(s) 45
AgsI TTSAA 1 cut(s) 253
AjnI CCWGG 1 cut(s) 116
AjuI GAANNNNNNNTTGG 1 cut(s) 354
AluBI AGCT 2 cut(s) 54, 365
AluI AGCT 2 cut(s) 54, 365
AlwI GGATC 2 cut(s) 191, 244
Ama87I CYCGRG 1 cut(s) 35
ApeKI GCWGC 1 cut(s) 54
AspLEI GCGC 1 cut(s) 268
AspS9I GGNCC 1 cut(s) 329
AvaI CYCGRG 1 cut(s) 35
AvaII GGWCC 1 cut(s) 329
BbsI GAAGAC 1 cut(s) 48
BbvI GCAGC 1 cut(s) 41
BccI CCATC 2 cut(s) 81, 329
BciT130I CCWGG 1 cut(s) 118
BclI TGATCA 1 cut(s) 27
BfmI CTRYAG 1 cut(s) 64
BisI GCNGC 2 cut(s) 55, 264
BlsI GCNGC 2 cut(s) 56, 265
Bme1390I CCNGG 1 cut(s) 118
Bme18I GGWCC 1 cut(s) 329
BmeT110I CYCGRG 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 329
BmrFI CCNGG 1 cut(s) 118
BmsI GCATC 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 48
BpmI CTGGAG 1 cut(s) 100
BpuEI CTTGAG 1 cut(s) 329
Bsc4I CCNNNNNNNGG 1 cut(s) 123
BseBI CCWGG 1 cut(s) 118
BseGI GGATG 1 cut(s) 303
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMII CTCAG 1 cut(s) 380
BseXI GCAGC 1 cut(s) 41
BsiHKCI CYCGRG 1 cut(s) 35
BslI CCNNNNNNNGG 1 cut(s) 123
BsoBI CYCGRG 1 cut(s) 35
Bsp143I GATC 3 cut(s) 27, 183, 236
BspACI CCGC 3 cut(s) 181, 263, 375
BspCNI CTCAG 1 cut(s) 379
BspHI TCATGA 1 cut(s) 186
BspMAI CTGCAG 1 cut(s) 68
BspPI GGATC 2 cut(s) 191, 244
BspQI GCTCTTC 1 cut(s) 171
BsrBI CCGCTC 1 cut(s) 181
BssMI GATC 3 cut(s) 27, 183, 236
BssNAI GTATAC 1 cut(s) 204
Bst1107I GTATAC 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 171
BstAPI GCANNNNNTGC 1 cut(s) 63
BstC8I GCNNGC 1 cut(s) 59
BstDEI CTNAG 2 cut(s) 102, 366
BstF5I GGATG 1 cut(s) 303
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 3 cut(s) 30, 186, 239
BstMBI GATC 3 cut(s) 27, 183, 236
BstMWI GCNNNNNNNGC 2 cut(s) 63, 362
BstNI CCWGG 1 cut(s) 118
BstNSI RCATGY 1 cut(s) 49
BstSCI CCNGG 1 cut(s) 116
BstSFI CTRYAG 1 cut(s) 64
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 1 cut(s) 48
BstZ17I GTATAC 1 cut(s) 204
BtsCI GGATG 1 cut(s) 303
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 1 cut(s) 61
Cac8I GCNNGC 1 cut(s) 59
CciI TCATGA 1 cut(s) 186
CfoI GCGC 1 cut(s) 268
Cfr13I GGNCC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 286
CviAII CATG 4 cut(s) 46, 187, 231, 269
CviJI RGCY 4 cut(s) 19, 54, 131, 365
CviKI_1 RGCY 4 cut(s) 19, 54, 131, 365
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 2 cut(s) 102, 366
DpnI GATC 3 cut(s) 29, 185, 238
DpnII GATC 3 cut(s) 27, 183, 236
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 329
Eco88I CYCGRG 1 cut(s) 35
EcoRII CCWGG 1 cut(s) 116
FaeI CATG 4 cut(s) 49, 190, 234, 272
FaiI YATR 9 cut(s) 47, 51, 123, 188, 204, 218, 232, 258, 270
FatI CATG 4 cut(s) 45, 186, 230, 268
FbaI TGATCA 1 cut(s) 27
FblI GTMKAC 2 cut(s) 87, 203
Fnu4HI GCNGC 2 cut(s) 55, 264
FokI GGATG 1 cut(s) 290
Fsp4HI GCNGC 2 cut(s) 55, 264
GlaI GCGC 1 cut(s) 267
GluI GCNGC 2 cut(s) 55, 264
GsuI CTGGAG 1 cut(s) 100
HhaI GCGC 1 cut(s) 268
Hin1II CATG 4 cut(s) 49, 190, 234, 272
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HinfI GANTC 2 cut(s) 315, 322
Hpy166II GTNNAC 2 cut(s) 88, 204
Hpy188I TCNGA 1 cut(s) 369
Hpy188III TCNNGA 6 cut(s) 35, 151, 187, 319, 332, 359
Hpy8I GTNNAC 2 cut(s) 88, 204
Hpy99I CGWCG 2 cut(s) 92, 95
HpyAV CCTTC 3 cut(s) 9, 121, 287
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 3 cut(s) 57, 66, 303
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 362
HpyF3I CTNAG 2 cut(s) 102, 366
HpySE526I ACGT 1 cut(s) 93
Hsp92II CATG 4 cut(s) 49, 190, 234, 272
HspAI GCGC 1 cut(s) 266
Ksp22I TGATCA 1 cut(s) 27
Kzo9I GATC 3 cut(s) 27, 183, 236
LguI GCTCTTC 1 cut(s) 171
LpnPI CCDG 6 cut(s) 103, 130, 180, 293, 332, 345
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 1 cut(s) 312
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 1 cut(s) 157
MalI GATC 3 cut(s) 29, 185, 238
MbiI CCGCTC 1 cut(s) 181
MboI GATC 3 cut(s) 27, 183, 236
MboII GAAGA 2 cut(s) 53, 188
MfeI CAATTG 1 cut(s) 351
MluCI AATT 1 cut(s) 351
MnlI CCTC 2 cut(s) 31, 253
MspR9I CCNGG 1 cut(s) 118
MunI CAATTG 1 cut(s) 351
MvaI CCWGG 1 cut(s) 118
MwoI GCNNNNNNNGC 2 cut(s) 63, 362
NdeII GATC 3 cut(s) 27, 183, 236
NlaIII CATG 4 cut(s) 49, 190, 234, 272
NmuCI GTSAC 1 cut(s) 157
NspI RCATGY 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 35
PagI TCATGA 1 cut(s) 186
PciI ACATGT 1 cut(s) 45
PciSI GCTCTTC 1 cut(s) 171
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 147
PfeI GAWTC 2 cut(s) 315, 322
PkrI GCNGC 2 cut(s) 56, 265
PscI ACATGT 1 cut(s) 45
Psp6I CCWGG 1 cut(s) 116
PspGI CCWGG 1 cut(s) 116
PspPI GGNCC 1 cut(s) 329
PstI CTGCAG 1 cut(s) 68
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SalI GTCGAC 1 cut(s) 86
SapI GCTCTTC 1 cut(s) 171
SatI GCNGC 2 cut(s) 55, 264
Sau3AI GATC 3 cut(s) 27, 183, 236
Sau96I GGNCC 1 cut(s) 329
ScrFI CCNGG 1 cut(s) 118
SetI ASST 4 cut(s) 56, 96, 122, 367
SfaNI GCATC 1 cut(s) 312
SfcI CTRYAG 1 cut(s) 64
Sfr274I CTCGAG 1 cut(s) 35
SinI GGWCC 1 cut(s) 329
SlaI CTCGAG 1 cut(s) 35
SmlI CTYRAG 2 cut(s) 35, 308
SmoI CTYRAG 2 cut(s) 35, 308
Sse9I AATT 1 cut(s) 351
SsiI CCGC 3 cut(s) 181, 263, 375
StyD4I CCNGG 1 cut(s) 116
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 1 cut(s) 96
TaqI TCGA 4 cut(s) 36, 87, 141, 360
TaqII GACCGA 1 cut(s) 317
TasI AATT 1 cut(s) 351
TatI WGTACW 1 cut(s) 285
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 2 cut(s) 315, 322
TscAI CASTG 1 cut(s) 68
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 1 cut(s) 54
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 1 cut(s) 219
TspRI CASTG 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 329
XceI RCATGY 1 cut(s) 49
XhoI CTCGAG 1 cut(s) 35
XmiI GTMKAC 2 cut(s) 87, 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.