pycom12g16720

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0017378
Physical Location & Seq
Forward (+)
19038212 .. 19042908
4697 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4158 bp
ATGTATGTCTTGATGGTAAACGTAGTTCGGCCGTCGGAATGCCGAACCCTGAAACCTACTTGCGAGTATCCAATCATAAAACCACTCGGCGTCCTGTGCGCCGAGCAATGTGACCTCTCGGTCGACCTCGACGCAGTGCTGTTTATCCAAACTGAAGGTGCTTCTCGGTCGGCTGATTCTGCGGCAGCAGTGCTGTTTATCCAAACTGAAGGTGCTCGCCGGGCGCTTCCACAGTGCTGTTTATCCAAACTGAAGGTGTGTCGGCAGGAAAAGAAAAGGAAAAATCTCAAGGTGTTTGAGAGGTTTTGCGCAGGGCAATTGTATGCTGATTTGCCCTATTCCTTGAACCGACTCCGATTTGGATTGGGAGTCCTTGTCCCATTTTGCTTCTTCCTCGAACAAACCTACTCCTACTGGGATTCTGAATTTTCCTTCTTTTCGGGACTTCGTTCCTTGCTGGTCGAGAATCCTAGTTGCACCAGGACTTCCTCGACCTTTCTATTTATCTGGACTACTTATGATAGGATTTGGCCGACCCTGTTAGCTGGCCCGGGCCCTATTATTCGGCCCATAGCATTTATCATCTCCTTGGGCCGAGCACATCCTGCTGCTCGGCCCAGCAATAATATTTTGGGCCCAAACATTGCCCCCTCGCTTCTGAGGTCGCCGACCGTTAACTCTTGGTCGGGCCTCGACCTTGAAGAATCCTGCGCATTTATGAGGCATGATTGCACTTTCCTCGTTGCCCCCAAAGCGTGGCCACGTGTCGATCCACCGCCGCTCTTAGGCCTTTCAATCGTGGGCCTTAAAACTGGCGTCGCACGTAATCGTGCGTCCTGGATTTTCGACTTCTCGATCCCCTCTTCGCAGGTTCCCAAAATATCCGAAATGATTGAGCCTTTCCCGGTTAAATTTCCCCCCAATTTGAACCAATACTTTTCGAATCCCAGACGTTTGATATTCATCCTGAAAGTCCCTCATCCTTGTTCGTTCAAATCAAACCTTCCAGTTCTTCCTTCTGCAATACCTTCTTTTTCCAGAAACATCTCTTCTCCACAAATGGCCTCCTTCATTTCAAAGCTCTCTGATGAGATCAACAAGTGCAAGGATTTCATCGATCGGAACGCCGTCAAGACGCTACGATTTGAAGAAGACATGGCCACGACCCACCAAATTCTGGGTCCCCTCTTCAGGGACGCAGTGCCGTCGTCTGTCACTAACCTGCTCAAGGAGCAGTGCCTAGCACCCCTGCTTCAAGGGTTTGACTGGTCGAAATGGTGCTTGGCTCGGCCTCAAGGAGCGTGGCCCTCGACCACCACGGCCTGGGCCGCCTGGGTAGACCGAATGAAATCCTTCTTCGGCAAAGAGTGGAAAGCCCTTGGTATCTATGATGCCATCCTGCTCTCGTCCATGGAGATCACCATGGACAATGAGCTTCTTATGGCGGCCTTGACCCTATGGTGTTCGGCCACCAACACCATGGTCCTTCCATTCGGACCTCTCGGCCCTACAATCCTCGACATCTCGGCCATTCTTGGGACTCCACCATCCGGTCTCCCAATCGATGCCGCTCTTTCTGGATGCCCCTCCCTCCTCGACCTGAAGGCGCTTTTCGACGAGCGGGCTGTCAAAACGCTCAGCCGAGGCGGTCGGGAGCCTTCAAGAGAAGAAGTTCAAAAGCTTCATAAGAACTTCTTCAACTACAATACTCTGGTTCCCCATTTTGCCGGCCGAGGCGAGGAGAGTCTCAGGAAGGGAGAACATGAAGCCTTCCTCTTCTATTGGTACAACAAATTTATTTGTTGTACCAGGTCGAACAAGTGCTTGGTCGAGAATATGCCAGTGGCCCAGGCCCTGGCTAGTGGTCACTCTCTGGCACTTAGCCCGGCTATTCTTGCCAACATTTTTCATTGCTTGGCCGAGACGACCATCAACAAGATTGACCCGCACCAGAATGGGCCCCTCTGGGTTTTCCAACTCTGGCTGCAGGTCTATTTTTCCACTCTTCGGCCTGAGGTCCCCGCCTTCCAGCGCTCGGCCGCCCTCGGCCCCCAACTTGCCTCTCGACCGTCTCGATGTTCTTTCGGACGACGAGTTCTTGGTGTGCCGGCGCCAGGAGTACCCTGCGTCAATCGGGCTCCCGACGGCAACTTGGGCCTCGGCCGAGGACGCGGGCCTTCGTCAGTCCTGGGGGTCAATCGTACTGGCTCGCGACCTTCCCCTTGGTTGCGACGCTCGCCGAGCCGGTTGAGAGGTCTACCAGCCCCACTTCGTCGCCCGACAGCTCGGCTACCTCCAAGGTTGCCCTGTACCCCTGATCGTCTCTCGTTCCCTCCTGAGCCGGGGACGTCTTTCTGGTTTTTCCGAGAGGGAGTGCCGGACGACGGAGCAGGAGTTTCAGGATCAGTGCAAGAAGTTCCGTCTTCGGCCAACCGTTCCGGAATCCTTGGGCACCGACACATTTGGGGACTGGTGGGACGAGTACGCTGGCGACTTCTTCGGCGCCTCGGCCGAAGACGTGGTGAGCAAAGTGAGTCGGTCCAAGGTATATTGTTGACTAATTGCTTTGCCAGTTCAGAAAAACTCACTTGTCCTTGTTTACTAATTGCTTTGCTACTTCAGGGGGTCGTGGGTCGAAAACTGTCGAGAAGGGGACCCTTGTCACCGAGCGGTCGGTCCCTGCCAAACGCCCCCACCAAGAGACCGAGCCGGCGGAAAAGGCCCATCGCCCTTCTAAACGCGTCAAGAAATTGGCGAAGAAGGGCGACCGGGAAATTCATGTCGTCCCCAGTGACACTATTGGGACGCCTGCTCCTGCCGGCTCTCCAACTGTGCCGGCTACCCCGGCATCTCCAACCCCGAATCCTCCAGCCGTTTCCCCGGCCGATCCAATCGTCGCACCCGCTCCAGCTTCGGAGCCGGTTGTGGTACCTGAAGTGGGGAAGTCGGCTGCGCCTGCCGAAGCACCCTTGTCTCCAACGGTCGTTTTGGAGGTATAATTCTGCTTCCACTTGCTTCTTTAGGTTTCTGGTCAGCGAGAGTGACGAAGTCCCGCTGCAACGTCGCCGTCGACCAGTCAACTCTCCTCCCGTCCATCCTCCTCCTGTAGTCGAGGTGACCGCTCGACCACGTCCTACTGCGGCGGATCGTGGTAAGAGACCCATGGTCGATCCTGAGGCCACGGCCGAGTCATCGATCCACCCGCAGGACGAAGACCCTCCCATTCCTCCACAGGAAGTTTCTTCGGCCTTTGTAAGTCTCGCTTTTTGTTGTCAGGTGCCCGAACATTCATTTCACCGGCAGTTTTATGCGCCGAGGGGCGTTGTCTATATTTCCTGGCCGCCGTCGTGGCCATCAAATGTAGATAACCTCCATCAGCCGCGTGAATTGCGTAGCGGTCTAAGGCACTGGGCTCGGCCATTAAGCTCCCTGGGTTCGAGCAATGACCCAGAGGGCATGGCCGAAGCACTATATCACGTCTTACTTTCCCTCACAGTATTCCTTTTATGTGCACAGCCATCGTGGGAAGTGGAGCTCGACGCTCTTCTTCAGAGCACGACCGGTGAGGTCGGCTCCTCTGCAGCTCCGGCCGAATCGGCGGGAGCCACGCCCGCCGAAGTCTTCGTGAAGCTGCACGAAGTCCTGTCTCTGACTGCTTCCCAAGTTCTCCGGTGCAAGGGCCTTGACTCTGTTGGAGAGTGTCTCAATGACCTCGCCAGCGGTGGTCACCTGAGCCCGGAGGGCATCTCTTTCCTAACCGACCTCCTAGAGCGGACTCGGCAACACTTTCTTATCTTTGAGCGTGCCCTACAGGCCGAGGACGATTTGAAGGTGGCCAAGGTTGCGCATGAGGCTGGCCGAGTAGAGATGGAGGCTTTCAAGGCAAAGAAAGCAAAGCTGACCGAGTTTGATCTCCAGATCTCTGACCTCCAGCGTCAGATTTCTGACCTTCAACGTCAAAGGTCGGCTGCTGCTTCCGAGCTTGAGAAGGATTTTGAGGCTAACAAGGCTCGGCTGACGGCCTTCGCTGCCGGCGCACGGCGTATCCAGCAACTACAGTGCAACAAGAAGACACGGCAGGCTGAGATAATTTTGAGCGAGGCGAAGTGGCTGGAGCTGAAAGCTGCCCTTGAGACTTACCTCCCCTCGACCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans Pkinase cNMP_binding

Protein Analysis

1386

Amino Acids

154.27

Weight (kDa)

9.63

Isoelectric Point (pI)

63.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 461 - 675 1.4e-31 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AatII GACGTC 1 cut(s) 2351
Acc16I TGCGCA 3 cut(s) 310, 712, 3846
Acc36I ACCTGC 3 cut(s) 859, 1230, 1978
Acc65I GGTACC 1 cut(s) 2928
AccB1I GGYRCC 5 cut(s) 2110, 2451, 2502, 2928, 3277
AccB7I CCANNNNNTGG 4 cut(s) 756, 1177, 1323, 1855
AccBSI CCGCTC 7 cut(s) 781, 1571, 1621, 2670, 2905, 3122, 3772
AccI GTMKAC 4 cut(s) 123, 1338, 2257, 3070
AccII CGCG 4 cut(s) 2172, 2212, 2741, 3382
AccIII TCCGGA 1 cut(s) 2438
AclWI GGATC 7 cut(s) 764, 850, 2410, 2881, 3153, 3164, 3190
AcsI RAATTY 5 cut(s) 425, 911, 1173, 1793, 2774
AcuI CTGAAG 8 cut(s) 174, 228, 272, 1174, 1622, 2603, 2954, 3533
AcvI CACGTG 1 cut(s) 764
AcyI GRCGYC 6 cut(s) 90, 816, 2111, 2348, 2503, 2806
AfaI GTAC 7 cut(s) 1787, 1807, 2121, 2203, 2311, 2484, 2930
AfeI AGCGCT 1 cut(s) 2033
AflIII ACRYGT 2 cut(s) 763, 2739
AgeI ACCGGT 1 cut(s) 3560
AhdI GACNNNNNGTC 1 cut(s) 3164
AjiI CACGTC 3 cut(s) 2519, 3131, 3478
AjuI GAANNNNNNNTTGG 4 cut(s) 1265, 1297, 1808, 1840
AloI GAACNNNNNNTCC 2 cut(s) 2079, 2111
Alw21I GWGCWC 5 cut(s) 217, 601, 3514, 3537, 3557
Alw44I GTGCAC 1 cut(s) 3510
AlwI GGATC 7 cut(s) 764, 850, 2410, 2881, 3153, 3164, 3190
AlwNI CAGNNNCTG 1 cut(s) 1855
Ama87I CYCGRG 1 cut(s) 550
Aor13HI TCCGGA 1 cut(s) 2438
Aor51HI AGCGCT 1 cut(s) 2033
ApaI GGGCCC 3 cut(s) 557, 638, 1962
ApaLI GTGCAC 1 cut(s) 3510
ApoI RAATTY 5 cut(s) 425, 911, 1173, 1793, 2774
ArsI GACNNNNNNTTYG 2 cut(s) 1165, 1197
AsiGI ACCGGT 1 cut(s) 3560
Asp700I GAANNNNTTC 3 cut(s) 1352, 1671, 1694
Asp718I GGTACC 1 cut(s) 2928
AsuHPI GGTGA 7 cut(s) 1411, 2534, 2655, 3127, 3287, 3575, 3719
AsuII TTCGAA 1 cut(s) 941
AvaI CYCGRG 1 cut(s) 550
AvaII GGWCC 7 cut(s) 1181, 1483, 1496, 2017, 2539, 2654, 2676
AxyI CCTNAGG 2 cut(s) 2013, 3174
BaeGI GKGCMC 7 cut(s) 557, 638, 1962, 2454, 3282, 3514, 3808
BalI TGGCCA 4 cut(s) 760, 1160, 3352, 3836
BanI GGYRCC 5 cut(s) 2110, 2451, 2502, 2928, 3277
BanII GRGCYC 7 cut(s) 557, 638, 1962, 2140, 3415, 3537, 3737
BbrPI CACGTG 1 cut(s) 764
BbsI GAAGAC 6 cut(s) 1158, 2415, 2521, 3219, 3613, 4076
Bbv12I GWGCWC 5 cut(s) 217, 601, 3514, 3537, 3557
BcgI CGANNNNNNTGC 8 cut(s) 721, 755, 1188, 1222, 3395, 3429, 3501, 3535
BciVI GTATCC 2 cut(s) 78, 4055
BfaI CTAG 4 cut(s) 471, 1241, 1860, 3767
BfmI CTRYAG 5 cut(s) 1985, 3105, 3579, 3809, 4055
BfoI RGCGCY 5 cut(s) 227, 1610, 2035, 2114, 2506
BfuAI ACCTGC 3 cut(s) 859, 1230, 1978
BfuI GTATCC 2 cut(s) 78, 4055
BglI GCCNNNNNGGC 4 cut(s) 3349, 3596, 3741, 3812
BglII AGATCT 1 cut(s) 3918
BlpI GCTNAGC 1 cut(s) 1637
Bme18I GGWCC 7 cut(s) 1181, 1483, 1496, 2017, 2539, 2654, 2676
BmeRI GACNNNNNGTC 1 cut(s) 3164
BmeT110I CYCGRG 1 cut(s) 550
BmgBI CACGTC 3 cut(s) 2519, 3131, 3478
BmrI ACTGGG 3 cut(s) 424, 2783, 3418
BmsI GCATC 5 cut(s) 1381, 1555, 1571, 2857, 3753
BmuI ACTGGG 3 cut(s) 424, 2783, 3418
BoxI GACNNNNGTC 1 cut(s) 2659
BpiI GAAGAC 6 cut(s) 1158, 2415, 2521, 3219, 3613, 4076
BplI GAGNNNNNCTC 6 cut(s) 3557, 3589, 3687, 3719, 3731, 3763
BpmI CTGGAG 5 cut(s) 2852, 2891, 3899, 3914, 4133
Bpu10I CCTNAGC 2 cut(s) 2337, 3731
Bpu1102I GCTNAGC 1 cut(s) 1637
Bpu14I TTCGAA 1 cut(s) 941
BpuEI CTTGAG 5 cut(s) 272, 1211, 1278, 4004, 4151
Bsa29I ATCGAT 3 cut(s) 1116, 1563, 3194
BsaAI YACGTR 2 cut(s) 764, 824
BsaBI GATNNNNATC 1 cut(s) 962
BsaHI GRCGYC 6 cut(s) 90, 816, 2111, 2348, 2503, 2806
BsaI GGTCTC 3 cut(s) 1559, 2695, 3151
BsaWI WCCGGW 4 cut(s) 1550, 2438, 3560, 3669
BsaXI ACNNNNNCTCC 6 cut(s) 360, 390, 2379, 2409, 2796, 2826
Bse21I CCTNAGG 2 cut(s) 2013, 3174
Bse3DI GCAATG 4 cut(s) 113, 642, 1909, 3448
Bse8I GATNNNNATC 1 cut(s) 962
BseAI TCCGGA 1 cut(s) 2438
BseCI ATCGAT 3 cut(s) 1116, 1563, 3194
BseGI GGATG 7 cut(s) 601, 963, 979, 1395, 1547, 1586, 3094
BseJI GATNNNNATC 1 cut(s) 962
BseMI GCAATG 4 cut(s) 113, 642, 1909, 3448
BseMII CTCAG 8 cut(s) 650, 1651, 1762, 2004, 2328, 3165, 3722, 4074
BseRI GAGGAG 5 cut(s) 1583, 1754, 3075, 3090, 3565
BseSI GKGCMC 7 cut(s) 557, 638, 1962, 2454, 3282, 3514, 3808
BseX3I CGGCCG 8 cut(s) 29, 1729, 2037, 2161, 2509, 2882, 3183, 3588
BseYI CCCAGC 1 cut(s) 617
BsgI GTGCAG 1 cut(s) 3617
Bsh1236I CGCG 4 cut(s) 2172, 2212, 2741, 3382
BshNI GGYRCC 5 cut(s) 2110, 2451, 2502, 2928, 3277
BshTI ACCGGT 1 cut(s) 3560
BshVI ATCGAT 3 cut(s) 1116, 1563, 3194
BsiHKAI GWGCWC 5 cut(s) 217, 601, 3514, 3537, 3557
BsiHKCI CYCGRG 1 cut(s) 550
BsmBI CGTCTC 3 cut(s) 1916, 2076, 2327
BsmI GAATGC 1 cut(s) 44
Bso31I GGTCTC 3 cut(s) 1559, 2695, 3151
BsoBI CYCGRG 1 cut(s) 550
Bsp119I TTCGAA 1 cut(s) 941
Bsp120I GGGCCC 3 cut(s) 553, 634, 1958
Bsp13I TCCGGA 1 cut(s) 2438
Bsp1720I GCTNAGC 1 cut(s) 1637
Bsp19I CCATGG 4 cut(s) 1410, 1422, 1479, 3162
Bsp68I TCGCGA 1 cut(s) 2212
BspCNI CTCAG 8 cut(s) 651, 1650, 1761, 2005, 2329, 3166, 3723, 4075
BspDI ATCGAT 3 cut(s) 1116, 1563, 3194
BspEI TCCGGA 1 cut(s) 2438
BspFNI CGCG 4 cut(s) 2172, 2212, 2741, 3382
BspMAI CTGCAG 2 cut(s) 1989, 3583
BspMI ACCTGC 3 cut(s) 859, 1230, 1978
BspPI GGATC 7 cut(s) 764, 850, 2410, 2881, 3153, 3164, 3190
BspQI GCTCTTC 1 cut(s) 3549
BspT104I TTCGAA 1 cut(s) 941
BspT107I GGYRCC 5 cut(s) 2110, 2451, 2502, 2928, 3277
BspTNI GGTCTC 3 cut(s) 1559, 2695, 3151
BsrBI CCGCTC 7 cut(s) 781, 1571, 1621, 2670, 2905, 3122, 3772
BsrDI GCAATG 4 cut(s) 113, 642, 1909, 3448
BssNI GRCGYC 6 cut(s) 90, 816, 2111, 2348, 2503, 2806
Bst4CI ACNGT 9 cut(s) 234, 673, 2070, 2435, 2643, 2832, 2982, 3496, 4059
Bst6I CTCTTC 6 cut(s) 868, 1054, 1193, 1781, 2010, 3549
BstACI GRCGYC 6 cut(s) 90, 816, 2111, 2348, 2503, 2806
BstAPI GCANNNNNTGC 1 cut(s) 605
BstBAI YACGTR 2 cut(s) 764, 824
BstBI TTCGAA 1 cut(s) 941
BstDSI CCRYGG 6 cut(s) 1317, 1410, 1422, 1479, 3162, 3180
BstEII GGTNACC 2 cut(s) 3115, 3725
BstENI CCTNNNNNAGG 1 cut(s) 1226
BstF5I GGATG 7 cut(s) 601, 963, 979, 1395, 1547, 1586, 3094
BstFNI CGCG 4 cut(s) 2172, 2212, 2741, 3382
BstH2I RGCGCY 5 cut(s) 227, 1610, 2035, 2114, 2506
BstPAI GACNNNNGTC 1 cut(s) 2659
BstPI GGTNACC 2 cut(s) 3115, 3725
BstSFI CTRYAG 5 cut(s) 1985, 3105, 3579, 3809, 4055
BstSLI GKGCMC 7 cut(s) 557, 638, 1962, 2454, 3282, 3514, 3808
BstUI CGCG 4 cut(s) 2172, 2212, 2741, 3382
BstV2I GAAGAC 6 cut(s) 1158, 2415, 2521, 3219, 3613, 4076
BstX2I RGATCY 1 cut(s) 3918
BstXI CCANNNNNNTGG 1 cut(s) 1480
BstYI RGATCY 1 cut(s) 3918
BstZI CGGCCG 8 cut(s) 29, 1729, 2037, 2161, 2509, 2882, 3183, 3588
Bsu15I ATCGAT 3 cut(s) 1116, 1563, 3194
Bsu36I CCTNAGG 2 cut(s) 2013, 3174
BsuI GTATCC 2 cut(s) 78, 4055
BsuTUI ATCGAT 3 cut(s) 1116, 1563, 3194
BtgI CCRYGG 6 cut(s) 1317, 1410, 1422, 1479, 3162, 3180
BtgZI GCGATG 1 cut(s) 2710
BtrI CACGTC 3 cut(s) 2519, 3131, 3478
BtsCI GGATG 7 cut(s) 601, 963, 979, 1395, 1547, 1586, 3094
BtsI GCAGTG 4 cut(s) 141, 195, 1206, 1241
BtuMI TCGCGA 1 cut(s) 2212
BveI ACCTGC 3 cut(s) 859, 1230, 1978
CaiI CAGNNNCTG 1 cut(s) 1855
Cfr9I CCCGGG 1 cut(s) 550
ClaI ATCGAT 3 cut(s) 1116, 1563, 3194
CsiI ACCWGGT 1 cut(s) 1808
Csp6I GTAC 7 cut(s) 1786, 1806, 2120, 2202, 2310, 2483, 2929
CspAI ACCGGT 1 cut(s) 3560
CspCI CAANNNNNGTGG 2 cut(s) 1283, 1318
CviQI GTAC 7 cut(s) 1786, 1806, 2120, 2202, 2310, 2483, 2929
DinI GGCGCC 2 cut(s) 2112, 2504
DrdI GACNNNNNNGTC 1 cut(s) 119
DriI GACNNNNNGTC 1 cut(s) 3164
DseDI GACNNNNNNGTC 1 cut(s) 119
EagI CGGCCG 8 cut(s) 29, 1729, 2037, 2161, 2509, 2882, 3183, 3588
Eam1104I CTCTTC 6 cut(s) 868, 1054, 1193, 1781, 2010, 3549
Eam1105I GACNNNNNGTC 1 cut(s) 3164
EarI CTCTTC 6 cut(s) 868, 1054, 1193, 1781, 2010, 3549
EciI GGCGGA 2 cut(s) 2728, 3158
Ecl136II GAGCTC 1 cut(s) 3535
EclXI CGGCCG 8 cut(s) 29, 1729, 2037, 2161, 2509, 2882, 3183, 3588
Eco147I AGGCCT 1 cut(s) 789
Eco24I GRGCYC 7 cut(s) 557, 638, 1962, 2140, 3415, 3537, 3737
Eco31I GGTCTC 3 cut(s) 1559, 2695, 3151
Eco47I GGWCC 7 cut(s) 1181, 1483, 1496, 2017, 2539, 2654, 2676
Eco47III AGCGCT 1 cut(s) 2033
Eco52I CGGCCG 8 cut(s) 29, 1729, 2037, 2161, 2509, 2882, 3183, 3588
Eco53kI GAGCTC 1 cut(s) 3535
Eco57I CTGAAG 8 cut(s) 174, 228, 272, 1174, 1622, 2603, 2954, 3533
Eco72I CACGTG 1 cut(s) 764
Eco81I CCTNAGG 2 cut(s) 2013, 3174
Eco88I CYCGRG 1 cut(s) 550
Eco91I GGTNACC 2 cut(s) 3115, 3725
EcoICRI GAGCTC 1 cut(s) 3535
EcoNI CCTNNNNNAGG 1 cut(s) 1226
EcoO109I RGGNCCY 7 cut(s) 554, 1181, 1852, 1959, 2017, 2654, 3679
EcoO65I GGTNACC 2 cut(s) 3115, 3725
EcoT38I GRGCYC 7 cut(s) 557, 638, 1962, 2140, 3415, 3537, 3737
EgeI GGCGCC 2 cut(s) 2112, 2504
EheI GGCGCC 2 cut(s) 2112, 2504
Esp3I CGTCTC 3 cut(s) 1916, 2076, 2327
FalI AAGNNNNNCTT 6 cut(s) 1679, 1711, 3247, 3279, 4113, 4145
FauI CCCGC 9 cut(s) 1614, 1953, 2029, 2165, 2910, 3060, 3210, 3592, 3619
FblI GTMKAC 4 cut(s) 123, 1338, 2257, 3070
FokI GGATG 7 cut(s) 588, 950, 966, 1382, 1534, 1593, 3081
FriOI GRGCYC 7 cut(s) 557, 638, 1962, 2140, 3415, 3537, 3737
FspBI CTAG 4 cut(s) 471, 1241, 1860, 3767
FspI TGCGCA 3 cut(s) 310, 712, 3846
GsaI CCCAGC 1 cut(s) 621
GsuI CTGGAG 5 cut(s) 2852, 2891, 3899, 3914, 4133
HaeII RGCGCY 5 cut(s) 227, 1610, 2035, 2114, 2506
Hin1I GRCGYC 6 cut(s) 90, 816, 2111, 2348, 2503, 2806
HincII GTYRAC 5 cut(s) 124, 676, 2556, 3071, 3079
HindII GTYRAC 5 cut(s) 124, 676, 2556, 3071, 3079
HindIII AAGCTT 1 cut(s) 1679
HpaI GTTAAC 1 cut(s) 676
HphI GGTGA 7 cut(s) 1411, 2534, 2655, 3127, 3287, 3575, 3719
HpyCH4III ACNGT 9 cut(s) 234, 673, 2070, 2435, 2643, 2832, 2982, 3496, 4059
Hsp92I GRCGYC 6 cut(s) 90, 816, 2111, 2348, 2503, 2806
KasI GGCGCC 2 cut(s) 2110, 2502
KflI GGGWCCC 2 cut(s) 1181, 2654
Kpn2I TCCGGA 1 cut(s) 2438
KpnI GGTACC 1 cut(s) 2932
KroI GCCGGC 6 cut(s) 1727, 2107, 2709, 2818, 2835, 4031
KroNI GCCGGC 6 cut(s) 1729, 2109, 2711, 2820, 2837, 4033
KspAI GTTAAC 1 cut(s) 676
LguI GCTCTTC 1 cut(s) 3549
LweI GCATC 5 cut(s) 1381, 1555, 1571, 2857, 3753
MabI ACCWGGT 1 cut(s) 1808
MaeI CTAG 4 cut(s) 471, 1241, 1860, 3767
MaeIII GTNAC 8 cut(s) 110, 1213, 1865, 2661, 2791, 3041, 3115, 3725
MbiI CCGCTC 7 cut(s) 781, 1571, 1621, 2670, 2905, 3122, 3772
MfeI CAATTG 1 cut(s) 317
MflI RGATCY 1 cut(s) 3918
MlsI TGGCCA 4 cut(s) 760, 1160, 3352, 3836
MluI ACGCGT 1 cut(s) 2739
MluNI TGGCCA 4 cut(s) 760, 1160, 3352, 3836
Mly113I GGCGCC 2 cut(s) 2111, 2503
MlyI GAGTC 8 cut(s) 345, 378, 1534, 1753, 2543, 3197, 3680, 3769
MmeI TCCRAC 6 cut(s) 15, 1999, 2851, 2878, 3001, 3673
Mox20I TGGCCA 4 cut(s) 760, 1160, 3352, 3836
MroI TCCGGA 1 cut(s) 2438
MroNI GCCGGC 6 cut(s) 1727, 2107, 2709, 2818, 2835, 4031
MroXI GAANNNNTTC 3 cut(s) 1352, 1671, 1694
MscI TGGCCA 4 cut(s) 760, 1160, 3352, 3836
MseI TTAA 4 cut(s) 675, 807, 909, 3422
MslI CAYNNNNRTG 1 cut(s) 1953
Msp20I TGGCCA 4 cut(s) 760, 1160, 3352, 3836
MspA1I CMGCKG 2 cut(s) 3055, 3720
MunI CAATTG 1 cut(s) 317
Mva1269I GAATGC 1 cut(s) 44
MvnI CGCG 4 cut(s) 2172, 2212, 2741, 3382
NaeI GCCGGC 6 cut(s) 1729, 2109, 2711, 2820, 2837, 4033
NarI GGCGCC 2 cut(s) 2111, 2503
NcoI CCATGG 4 cut(s) 1410, 1422, 1479, 3162
NgoMIV GCCGGC 6 cut(s) 1727, 2107, 2709, 2818, 2835, 4031
NmuCI GTSAC 8 cut(s) 110, 1213, 1865, 2661, 2791, 3041, 3115, 3725
NruI TCGCGA 1 cut(s) 2212
NsbI TGCGCA 3 cut(s) 310, 712, 3846
NspV TTCGAA 1 cut(s) 941
PasI CCCWGGG 1 cut(s) 3430
PceI AGGCCT 1 cut(s) 789
PciSI GCTCTTC 1 cut(s) 3549
PcsI WCGNNNNNNNCGW 2 cut(s) 2071, 3067
PctI GAATGC 1 cut(s) 44
PdiI GCCGGC 6 cut(s) 1729, 2109, 2711, 2820, 2837, 4033
PdmI GAANNNNTTC 3 cut(s) 1352, 1671, 1694
PfeI GAWTC 8 cut(s) 176, 419, 466, 704, 943, 2442, 2862, 3593
PflFI GACNNNGTC 2 cut(s) 3047, 3129
PflMI CCANNNNNTGG 4 cut(s) 756, 1177, 1323, 1855
PfoI TCCNGGA 1 cut(s) 836
PinAI ACCGGT 1 cut(s) 3560
Ple19I CGATCG 1 cut(s) 1120
PleI GAGTC 8 cut(s) 345, 377, 1534, 1752, 2542, 3196, 3680, 3769
PluTI GGCGCC 2 cut(s) 2114, 2506
PmaCI CACGTG 1 cut(s) 764
PmlI CACGTG 1 cut(s) 764
PpsI GAGTC 8 cut(s) 345, 377, 1534, 1752, 2542, 3196, 3680, 3769
Ppu21I YACGTR 2 cut(s) 764, 824
PpuMI RGGWCCY 3 cut(s) 1181, 2017, 2654
PshAI GACNNNNGTC 1 cut(s) 2659
Psp124BI GAGCTC 1 cut(s) 3537
Psp5II RGGWCCY 3 cut(s) 1181, 2017, 2654
PspCI CACGTG 1 cut(s) 764
PspEI GGTNACC 2 cut(s) 3115, 3725
PspFI CCCAGC 1 cut(s) 617
PspOMI GGGCCC 3 cut(s) 553, 634, 1958
PspPPI RGGWCCY 3 cut(s) 1181, 2017, 2654
PsrI GAACNNNNNNTAC 2 cut(s) 9, 41
PstI CTGCAG 2 cut(s) 1989, 3583
PstNI CAGNNNCTG 1 cut(s) 1855
PsuI RGATCY 1 cut(s) 3918
PsyI GACNNNGTC 2 cut(s) 3047, 3129
PvuI CGATCG 1 cut(s) 1120
RruI TCGCGA 1 cut(s) 2212
RsaI GTAC 7 cut(s) 1787, 1807, 2121, 2203, 2311, 2484, 2930
RsaNI GTAC 7 cut(s) 1786, 1806, 2120, 2202, 2310, 2483, 2929
RseI CAYNNNNRTG 1 cut(s) 1953
SacI GAGCTC 1 cut(s) 3537
SalI GTCGAC 2 cut(s) 122, 3069
SapI GCTCTTC 1 cut(s) 3549
SaqAI TTAA 4 cut(s) 675, 807, 909, 3422
SchI GAGTC 8 cut(s) 345, 378, 1534, 1753, 2543, 3197, 3680, 3769
SexAI ACCWGGT 1 cut(s) 1808
SfaNI GCATC 5 cut(s) 1381, 1555, 1571, 2857, 3753
SfcI CTRYAG 5 cut(s) 1985, 3105, 3579, 3809, 4055
SfoI GGCGCC 2 cut(s) 2112, 2504
SfuI TTCGAA 1 cut(s) 941
SinI GGWCC 7 cut(s) 1181, 1483, 1496, 2017, 2539, 2654, 2676
SmaI CCCGGG 1 cut(s) 552
SmiMI CAYNNNNRTG 1 cut(s) 1953
SmlI CTYRAG 5 cut(s) 287, 1226, 1293, 3983, 4130
SmoI CTYRAG 5 cut(s) 287, 1226, 1293, 3983, 4130
SrfI GCCCGGGC 1 cut(s) 552
SseBI AGGCCT 1 cut(s) 789
SspDI GGCGCC 2 cut(s) 2110, 2502
SspI AATATT 1 cut(s) 628
SspMI CTAG 4 cut(s) 471, 1241, 1860, 3767
SstI GAGCTC 1 cut(s) 3537
StuI AGGCCT 1 cut(s) 789
TaaI ACNGT 9 cut(s) 234, 673, 2070, 2435, 2643, 2832, 2982, 3496, 4059
TaqII GACCGA 7 cut(s) 109, 156, 1356, 2527, 2664, 2719, 3917
TauI GCSGC 9 cut(s) 185, 781, 1331, 1448, 1571, 2042, 3143, 3343, 3382
TfiI GAWTC 8 cut(s) 176, 419, 466, 704, 943, 2442, 2862, 3593
Tru1I TTAA 4 cut(s) 675, 807, 909, 3422
Tru9I TTAA 4 cut(s) 675, 807, 909, 3422
TseFI GTSAC 8 cut(s) 110, 1213, 1865, 2661, 2791, 3041, 3115, 3725
Tsp45I GTSAC 8 cut(s) 110, 1213, 1865, 2661, 2791, 3041, 3115, 3725
TspDTI ATGAA 9 cut(s) 952, 1060, 1102, 1361, 1673, 1779, 1898, 2767, 3279
TspGWI ACGGA 2 cut(s) 2400, 2409
TspMI CCCGGG 1 cut(s) 550
Tth111I GACNNNGTC 2 cut(s) 3047, 3129
Van91I CCANNNNNTGG 4 cut(s) 756, 1177, 1323, 1855
VneI GTGCAC 1 cut(s) 3510
VpaK11BI GGWCC 7 cut(s) 1181, 1483, 1496, 2017, 2539, 2654, 2676
XagI CCTNNNNNAGG 1 cut(s) 1226
XapI RAATTY 5 cut(s) 425, 911, 1173, 1793, 2774
XcmI CCANNNNNNNNNTGG 1 cut(s) 1477
XmaI CCCGGG 1 cut(s) 550
XmiI GTMKAC 4 cut(s) 123, 1338, 2257, 3070
XmnI GAANNNNTTC 3 cut(s) 1352, 1671, 1694
XspI CTAG 4 cut(s) 471, 1241, 1860, 3767
ZraI GACGTC 1 cut(s) 2349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.