pycom15g33860

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0017378
Physical Location & Seq
Forward (+)
33405563 .. 33406250
688 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g33860.3

Sequence Viewer

Length: 606 bp
ATGTCGAGAGCGAGACTCTCCTCCCATCTGTCCTTCTCCGGTAGTCGAGGCGGCCACTCGACCGACTCCTCCAGTGGCGGATCGTGGCAAGAGACCCATGGATGCAGATCTCCCCATTCCTCCGCAGGAAGTCTCTTCAGCCTTTGTAAGTCTTTCTTCTTTTGTGTTGATTCTCTTTTGATACATGTTTTTAAAACCATTAAATGTCAGGTGCCCGAACATTCATTTCACCGGCAATTTTATGCGCCGAGGGGCGTTGTTTATATTTCCTGGCCGCCATCGTGGCCATCAAATGTAGATGACCTCCATCGGCCGCGTGAATTGCGTAGCGGTCTGAGGCACTGGGCTCGGCCATTAAGTTCCCTGGGTTCGAGCAATGACCCAGGGGGCATGGCCGAAGTCTCTTCTCGGCAGGTCTCAAACATTCTCCTTTTTCCTTTATGCACTACATCACGTCTTTACTGTTCTTCACCAAATTTTCCTCTTATGTGCAGCCGTCGTGGGAAATGGAGCTTGACGCTCTACTCCAGAGCACGACCGGTGAGGCCGGCTCCTCTGCTGCTCCGGCCGAATCGGTGGGAGCCACGCCCGCCGAAGTCTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans Pkinase cNMP_binding

Protein Analysis

202

Amino Acids

22.56

Weight (kDa)

10.63

Isoelectric Point (pI)

68.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 403
AccB1I GGYRCC 1 cut(s) 211
AccII CGCG 1 cut(s) 316
AciI CCGC 7 cut(s) 51, 78, 123, 275, 314, 330, 590
AclWI GGATC 1 cut(s) 88
AcoI YGGCCR 7 cut(s) 52, 272, 284, 311, 350, 393, 566
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 1 cut(s) 121
AflIII ACRYGT 1 cut(s) 184
AgeI ACCGGT 1 cut(s) 538
AjiI CACGTC 1 cut(s) 455
AjnI CCWGG 3 cut(s) 269, 363, 382
AluBI AGCT 1 cut(s) 513
AluI AGCT 1 cut(s) 513
Alw21I GWGCWC 1 cut(s) 535
Alw26I GTCTC 5 cut(s) 7, 86, 137, 406, 421
AlwI GGATC 1 cut(s) 88
AoxI GGCC 8 cut(s) 52, 272, 284, 311, 350, 393, 545, 566
ApeKI GCWGC 2 cut(s) 492, 559
ApoI RAATTY 1 cut(s) 475
AsiGI ACCGGT 1 cut(s) 538
AspLEI GCGC 1 cut(s) 247
AsuHPI GGTGA 3 cut(s) 221, 462, 553
BaeGI GKGCMC 1 cut(s) 216
BalI TGGCCA 1 cut(s) 286
BanI GGYRCC 1 cut(s) 211
BanII GRGCYC 1 cut(s) 349
BbsI GAAGAC 1 cut(s) 591
Bbv12I GWGCWC 1 cut(s) 535
BbvI GCAGC 2 cut(s) 504, 546
BccI CCATC 4 cut(s) 33, 286, 295, 315
BceAI ACGGC 1 cut(s) 480
BcgI CGANNNNNNTGC 2 cut(s) 329, 363
BciT130I CCWGG 3 cut(s) 271, 365, 384
BcoDI GTCTC 5 cut(s) 7, 86, 137, 406, 421
BfuAI ACCTGC 1 cut(s) 403
BglI GCCNNNNNGGC 1 cut(s) 283
BglII AGATCT 1 cut(s) 107
BisI GCNGC 5 cut(s) 52, 275, 314, 493, 560
BlsI GCNGC 5 cut(s) 53, 276, 315, 494, 561
Bme1390I CCNGG 3 cut(s) 271, 365, 384
BmgBI CACGTC 1 cut(s) 455
BmiI GGNNCC 3 cut(s) 213, 552, 582
BmrFI CCNGG 3 cut(s) 271, 365, 384
BmrI ACTGGG 1 cut(s) 352
BmsI GCATC 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 352
BpiI GAAGAC 1 cut(s) 591
BplI GAGNNNNNCTC 3 cut(s) 32, 535, 567
BpmI CTGGAG 2 cut(s) 55, 511
BsaBI GATNNNNATC 1 cut(s) 106
BsaI GGTCTC 2 cut(s) 86, 421
BsaJI CCNNGG 6 cut(s) 97, 248, 363, 364, 382, 383
BsaWI WCCGGW 2 cut(s) 38, 538
Bse118I RCCGGY 3 cut(s) 231, 538, 547
Bse1I ACTGG 2 cut(s) 72, 347
Bse3DI GCAATG 1 cut(s) 382
Bse8I GATNNNNATC 1 cut(s) 106
BseBI CCWGG 3 cut(s) 271, 365, 384
BseDI CCNNGG 6 cut(s) 97, 248, 363, 364, 382, 383
BseGI GGATG 1 cut(s) 107
BseJI GATNNNNATC 1 cut(s) 106
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 1 cut(s) 326
BseNI ACTGG 2 cut(s) 72, 347
BseRI GAGGAG 3 cut(s) 10, 58, 543
BseSI GKGCMC 1 cut(s) 216
BseX3I CGGCCG 2 cut(s) 311, 566
BseXI GCAGC 2 cut(s) 504, 546
BsgI GTGCAG 1 cut(s) 511
Bsh1236I CGCG 1 cut(s) 316
Bsh1285I CGRYCG 4 cut(s) 63, 314, 539, 569
BshFI GGCC 8 cut(s) 54, 274, 286, 313, 352, 395, 547, 568
BshNI GGYRCC 1 cut(s) 211
BshTI ACCGGT 1 cut(s) 538
BsiEI CGRYCG 4 cut(s) 63, 314, 539, 569
BsiHKAI GWGCWC 1 cut(s) 535
BsiSI CCGG 5 cut(s) 39, 232, 539, 548, 565
BsmAI GTCTC 5 cut(s) 7, 86, 137, 406, 421
BsnI GGCC 8 cut(s) 54, 274, 286, 313, 352, 395, 547, 568
Bso31I GGTCTC 2 cut(s) 86, 421
Bsp1286I GDGCHC 3 cut(s) 216, 349, 535
Bsp143I GATC 2 cut(s) 80, 107
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 7 cut(s) 51, 78, 123, 275, 314, 330, 590
BspANI GGCC 8 cut(s) 54, 274, 286, 313, 352, 395, 547, 568
BspCNI CTCAG 1 cut(s) 327
BspFNI CGCG 1 cut(s) 316
BspLI GGNNCC 3 cut(s) 213, 552, 582
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 211
BspTNI GGTCTC 2 cut(s) 86, 421
BsrDI GCAATG 1 cut(s) 382
BsrFI RCCGGY 3 cut(s) 231, 538, 547
BsrI ACTGG 2 cut(s) 72, 347
BssAI RCCGGY 3 cut(s) 231, 538, 547
BssECI CCNNGG 6 cut(s) 97, 248, 363, 364, 382, 383
BssMI GATC 2 cut(s) 80, 107
BssT1I CCWWGG 1 cut(s) 97
Bst2UI CCWGG 3 cut(s) 271, 365, 384
Bst4CI ACNGT 1 cut(s) 464
Bst6I CTCTTC 2 cut(s) 140, 409
BstC8I GCNNGC 2 cut(s) 549, 590
BstDEI CTNAG 1 cut(s) 335
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 1 cut(s) 107
BstFNI CGCG 1 cut(s) 316
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 2 cut(s) 83, 110
BstMAI GTCTC 5 cut(s) 7, 86, 137, 406, 421
BstMBI GATC 2 cut(s) 80, 107
BstMCI CGRYCG 4 cut(s) 63, 314, 539, 569
BstMWI GCNNNNNNNGC 4 cut(s) 283, 322, 565, 589
BstNI CCWGG 3 cut(s) 271, 365, 384
BstNSI RCATGY 1 cut(s) 188
BstSCI CCNGG 3 cut(s) 269, 363, 382
BstSLI GKGCMC 1 cut(s) 216
BstUI CGCG 1 cut(s) 316
BstV1I GCAGC 2 cut(s) 504, 546
BstV2I GAAGAC 1 cut(s) 591
BstX2I RGATCY 1 cut(s) 107
BstYI RGATCY 1 cut(s) 107
BstZI CGGCCG 2 cut(s) 311, 566
BsuRI GGCC 8 cut(s) 54, 274, 286, 313, 352, 395, 547, 568
BtgI CCRYGG 1 cut(s) 97
BtrI CACGTC 1 cut(s) 455
BtsCI GGATG 1 cut(s) 107
BtsIMutI CAGTG 2 cut(s) 79, 340
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 2 cut(s) 549, 590
CfoI GCGC 1 cut(s) 247
Cfr10I RCCGGY 3 cut(s) 231, 538, 547
CseI GACGC 1 cut(s) 526
CspAI ACCGGT 1 cut(s) 538
CviAII CATG 3 cut(s) 98, 185, 391
DdeI CTNAG 1 cut(s) 335
DpnI GATC 2 cut(s) 82, 109
DpnII GATC 2 cut(s) 80, 107
DraI TTTAAA 1 cut(s) 193
EaeI YGGCCR 7 cut(s) 52, 272, 284, 311, 350, 393, 566
EagI CGGCCG 2 cut(s) 311, 566
Eam1104I CTCTTC 2 cut(s) 140, 409
EarI CTCTTC 2 cut(s) 140, 409
EciI GGCGGA 1 cut(s) 93
EclXI CGGCCG 2 cut(s) 311, 566
Eco130I CCWWGG 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 349
Eco31I GGTCTC 2 cut(s) 86, 421
Eco52I CGGCCG 2 cut(s) 311, 566
Eco57I CTGAAG 1 cut(s) 121
EcoRII CCWGG 3 cut(s) 269, 363, 382
EcoT14I CCWWGG 1 cut(s) 97
EcoT38I GRGCYC 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 97
FaeI CATG 3 cut(s) 101, 188, 394
FaiI YATR 7 cut(s) 99, 186, 243, 264, 392, 442, 488
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FatI CATG 3 cut(s) 97, 184, 390
FauI CCCGC 1 cut(s) 597
Fnu4HI GCNGC 5 cut(s) 52, 275, 314, 493, 560
FokI GGATG 1 cut(s) 114
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 5 cut(s) 52, 275, 314, 493, 560
GlaI GCGC 1 cut(s) 246
GluI GCNGC 5 cut(s) 52, 275, 314, 493, 560
GsuI CTGGAG 2 cut(s) 55, 511
HaeIII GGCC 8 cut(s) 54, 274, 286, 313, 352, 395, 547, 568
HapII CCGG 5 cut(s) 39, 232, 539, 548, 565
HgaI GACGC 1 cut(s) 526
HhaI GCGC 1 cut(s) 247
Hin1II CATG 3 cut(s) 101, 188, 394
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HinfI GANTC 4 cut(s) 15, 65, 170, 571
HpaII CCGG 5 cut(s) 39, 232, 539, 548, 565
HphI GGTGA 3 cut(s) 221, 462, 553
Hpy188I TCNGA 1 cut(s) 336
Hpy188III TCNNGA 3 cut(s) 6, 528, 603
Hpy99I CGWCG 1 cut(s) 501
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 1 cut(s) 464
HpyCH4IV ACGT 1 cut(s) 454
HpyCH4V TGCA 3 cut(s) 105, 444, 492
HpyF10VI GCNNNNNNNGC 4 cut(s) 283, 322, 565, 589
HpyF3I CTNAG 1 cut(s) 335
HpySE526I ACGT 1 cut(s) 454
Hsp92II CATG 3 cut(s) 101, 188, 394
HspAI GCGC 1 cut(s) 245
KroI GCCGGC 1 cut(s) 547
KroNI GCCGGC 1 cut(s) 549
Kzo9I GATC 2 cut(s) 80, 107
LmnI GCTCC 4 cut(s) 510, 556, 567, 580
Lsp1109I GCAGC 2 cut(s) 504, 546
LweI GCATC 1 cut(s) 92
MaeII ACGT 1 cut(s) 454
MalI GATC 2 cut(s) 82, 109
MboI GATC 2 cut(s) 80, 107
MboII GAAGA 5 cut(s) 127, 148, 396, 459, 591
MflI RGATCY 1 cut(s) 107
MhlI GDGCHC 3 cut(s) 216, 349, 535
MlsI TGGCCA 1 cut(s) 286
MluCI AATT 3 cut(s) 236, 320, 475
MluNI TGGCCA 1 cut(s) 286
MlyI GAGTC 2 cut(s) 9, 59
Mox20I TGGCCA 1 cut(s) 286
MroNI GCCGGC 1 cut(s) 547
MscI TGGCCA 1 cut(s) 286
MseI TTAA 3 cut(s) 192, 201, 356
Msp20I TGGCCA 1 cut(s) 286
MspI CCGG 5 cut(s) 39, 232, 539, 548, 565
MspR9I CCNGG 3 cut(s) 271, 365, 384
MvaI CCWGG 3 cut(s) 271, 365, 384
MvnI CGCG 1 cut(s) 316
MwoI GCNNNNNNNGC 4 cut(s) 283, 322, 565, 589
NaeI GCCGGC 1 cut(s) 549
NcoI CCATGG 1 cut(s) 97
NdeII GATC 2 cut(s) 80, 107
NgoMIV GCCGGC 1 cut(s) 547
NlaIII CATG 3 cut(s) 101, 188, 394
NlaIV GGNNCC 3 cut(s) 213, 552, 582
NmeAIII GCCGAG 3 cut(s) 273, 328, 388
NspI RCATGY 1 cut(s) 188
PasI CCCWGGG 2 cut(s) 364, 383
PciI ACATGT 1 cut(s) 184
PdiI GCCGGC 1 cut(s) 549
PfeI GAWTC 2 cut(s) 170, 571
PinAI ACCGGT 1 cut(s) 538
PkrI GCNGC 5 cut(s) 53, 276, 315, 494, 561
PleI GAGTC 2 cut(s) 9, 59
PpsI GAGTC 2 cut(s) 9, 59
PscI ACATGT 1 cut(s) 184
Psp6I CCWGG 3 cut(s) 269, 363, 382
PspGI CCWGG 3 cut(s) 269, 363, 382
PspN4I GGNNCC 3 cut(s) 213, 552, 582
PsuI RGATCY 1 cut(s) 107
SaqAI TTAA 3 cut(s) 192, 201, 356
SatI GCNGC 5 cut(s) 52, 275, 314, 493, 560
Sau3AI GATC 2 cut(s) 80, 107
SchI GAGTC 2 cut(s) 9, 59
ScrFI CCNGG 3 cut(s) 271, 365, 384
SduI GDGCHC 3 cut(s) 216, 349, 535
SetI ASST 5 cut(s) 213, 306, 417, 457, 515
SfaNI GCATC 1 cut(s) 92
Sse9I AATT 3 cut(s) 236, 320, 475
SsiI CCGC 7 cut(s) 51, 78, 123, 275, 314, 330, 590
StyD4I CCNGG 3 cut(s) 269, 363, 382
StyI CCWWGG 1 cut(s) 97
TaaI ACNGT 1 cut(s) 464
TaiI ACGT 1 cut(s) 457
TaqI TCGA 4 cut(s) 5, 46, 59, 371
TaqII GACCGA 1 cut(s) 77
TasI AATT 3 cut(s) 236, 320, 475
TauI GCSGC 3 cut(s) 54, 277, 316
TfiI GAWTC 2 cut(s) 170, 571
Tru1I TTAA 3 cut(s) 192, 201, 356
Tru9I TTAA 3 cut(s) 192, 201, 356
TscAI CASTG 2 cut(s) 79, 347
TseI GCWGC 2 cut(s) 492, 559
TspDTI ATGAA 1 cut(s) 213
TspRI CASTG 2 cut(s) 79, 347
XapI RAATTY 1 cut(s) 475
XceI RCATGY 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.