pycom12g16970

Macrophage migration inhibitory factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007982
Physical Location & Seq
Reverse (-)
19239822 .. 19241515
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 348 bp
ATGCCGTGCCTGAACATTTCAGCTAACGTGAACCTGGACGGCGTCGACACCTCCGCCGTCCTCTCCGAAGCCACCTCCACCGTCGCCAAGATCATCGGCAAACCCGAGGCTTATGTGATGATTGTTCTGAAGGGATCGGTACCCATATCTTTTGGCGGGACTGAGGAGCCAGCTGCCTATGGGGAGTTGGTCTCCATTGGCGGCCTTAATCCTGATGTGAACAAGAAGTTGAGTGCTGCAATTGCTGCAATTCTCGAGACAAAGTTGTCCGTGCCCAAGTCACGATTTTTTCTCAAGTTCTATGACACCAAGGGTTCCAACTTTGGATGGAACGGGTCTACCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00350 ko00360 map00350 map00360
Pfam Domains
Protein Families
MIF

Protein Analysis

116

Amino Acids

12.06

Weight (kDa)

6.19

Isoelectric Point (pI)

31.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 2 - 115 1.4e-22 Macrophage migration inhibitory factor (MIF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 265
Acc65I GGTACC 1 cut(s) 139
AccB1I GGYRCC 1 cut(s) 139
AccI GTMKAC 2 cut(s) 45, 338
AciI CCGC 3 cut(s) 54, 156, 201
AclWI GGATC 1 cut(s) 142
AcuI CTGAAG 1 cut(s) 149
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 1 cut(s) 141
AjnI CCWGG 1 cut(s) 33
AluBI AGCT 2 cut(s) 23, 173
AluI AGCT 2 cut(s) 23, 173
Alw26I GTCTC 2 cut(s) 196, 251
AlwI GGATC 1 cut(s) 142
Ama87I CYCGRG 2 cut(s) 104, 254
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 3 cut(s) 173, 236, 245
Asp718I GGTACC 1 cut(s) 139
AvaI CYCGRG 2 cut(s) 104, 254
BaeGI GKGCMC 1 cut(s) 276
BanI GGYRCC 1 cut(s) 139
BbvI GCAGC 3 cut(s) 160, 223, 232
BccI CCATC 1 cut(s) 321
BceAI ACGGC 2 cut(s) 41, 55
BciT130I CCWGG 1 cut(s) 35
BcoDI GTCTC 2 cut(s) 196, 251
BisI GCNGC 4 cut(s) 174, 202, 237, 246
BlsI GCNGC 4 cut(s) 175, 203, 238, 247
Bme1390I CCNGG 1 cut(s) 35
BmeT110I CYCGRG 2 cut(s) 104, 254
BmiI GGNNCC 3 cut(s) 141, 168, 316
BmrFI CCNGG 1 cut(s) 35
BplI GAGNNNNNCTC 2 cut(s) 176, 208
BpuEI CTTGAG 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 196
BsaJI CCNNGG 2 cut(s) 105, 309
BseBI CCWGG 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 105, 309
BseGI GGATG 1 cut(s) 332
BseMII CTCAG 1 cut(s) 153
BseRI GAGGAG 1 cut(s) 179
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 3 cut(s) 160, 223, 232
BshFI GGCC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 139
BsiHKCI CYCGRG 2 cut(s) 104, 254
BslFI GGGAC 1 cut(s) 172
BsmAI GTCTC 2 cut(s) 196, 251
BsmFI GGGAC 1 cut(s) 172
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 196
BsoBI CYCGRG 2 cut(s) 104, 254
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 2 cut(s) 90, 134
BspACI CCGC 3 cut(s) 54, 156, 201
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 1 cut(s) 154
BspLI GGNNCC 3 cut(s) 141, 168, 316
BspPI GGATC 1 cut(s) 142
BspT107I GGYRCC 1 cut(s) 139
BspTNI GGTCTC 1 cut(s) 196
BssECI CCNNGG 2 cut(s) 105, 309
BssMI GATC 2 cut(s) 90, 134
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 1 cut(s) 82
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 245
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 1 cut(s) 162
BstF5I GGATG 1 cut(s) 332
BstKTI GATC 2 cut(s) 93, 137
BstMAI GTCTC 2 cut(s) 196, 251
BstMBI GATC 2 cut(s) 90, 134
BstMWI GCNNNNNNNGC 2 cut(s) 242, 245
BstNI CCWGG 1 cut(s) 35
BstSCI CCNGG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 276
BstV1I GCAGC 3 cut(s) 160, 223, 232
BsuRI GGCC 1 cut(s) 204
BtsCI GGATG 1 cut(s) 332
Cac8I GCNNGC 1 cut(s) 171
CseI GACGC 1 cut(s) 31
Csp6I GTAC 1 cut(s) 140
CviJI RGCY 6 cut(s) 23, 71, 110, 169, 173, 204
CviKI_1 RGCY 6 cut(s) 23, 71, 110, 169, 173, 204
CviQI GTAC 1 cut(s) 140
DdeI CTNAG 1 cut(s) 162
DpnI GATC 2 cut(s) 92, 136
DpnII GATC 2 cut(s) 90, 134
DrdI GACNNNNNNGTC 1 cut(s) 265
DseDI GACNNNNNNGTC 1 cut(s) 265
EciI GGCGGA 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 309
Eco31I GGTCTC 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 149
Eco88I CYCGRG 2 cut(s) 104, 254
EcoRII CCWGG 1 cut(s) 33
EcoT14I CCWWGG 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 309
FaiI YATR 4 cut(s) 114, 146, 180, 303
FaqI GGGAC 1 cut(s) 172
FauI CCCGC 1 cut(s) 149
FblI GTMKAC 2 cut(s) 45, 338
Fnu4HI GCNGC 4 cut(s) 174, 202, 237, 246
FokI GGATG 1 cut(s) 339
Fsp4HI GCNGC 4 cut(s) 174, 202, 237, 246
GluI GCNGC 4 cut(s) 174, 202, 237, 246
HaeIII GGCC 1 cut(s) 204
HgaI GACGC 1 cut(s) 31
Hin1I GRCGYC 1 cut(s) 42
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
Hpy166II GTNNAC 4 cut(s) 31, 46, 220, 339
Hpy188I TCNGA 2 cut(s) 67, 129
Hpy188III TCNNGA 4 cut(s) 212, 254, 256, 282
Hpy8I GTNNAC 4 cut(s) 31, 46, 220, 339
Hpy99I CGWCG 2 cut(s) 47, 86
HpyAV CCTTC 1 cut(s) 124
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 2 cut(s) 239, 248
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 245
HpyF3I CTNAG 1 cut(s) 162
HpySE526I ACGT 1 cut(s) 27
Hsp92I GRCGYC 1 cut(s) 42
KpnI GGTACC 1 cut(s) 143
Kzo9I GATC 2 cut(s) 90, 134
LmnI GCTCC 1 cut(s) 166
LpnPI CCDG 5 cut(s) 20, 23, 47, 183, 225
Lsp1109I GCAGC 3 cut(s) 160, 223, 232
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 279
MalI GATC 2 cut(s) 92, 136
MboI GATC 2 cut(s) 90, 134
MfeI CAATTG 1 cut(s) 240
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 2 cut(s) 240, 249
MmeI TCCRAC 1 cut(s) 342
MnlI CCTC 5 cut(s) 61, 71, 85, 100, 157
MseI TTAA 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 173
MspR9I CCNGG 1 cut(s) 35
MunI CAATTG 1 cut(s) 240
MvaI CCWGG 1 cut(s) 35
MwoI GCNNNNNNNGC 2 cut(s) 242, 245
NdeII GATC 2 cut(s) 90, 134
NlaIV GGNNCC 3 cut(s) 141, 168, 316
NmuCI GTSAC 1 cut(s) 279
PaeR7I CTCGAG 1 cut(s) 254
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PflFI GACNNNGTC 1 cut(s) 41
PkrI GCNGC 4 cut(s) 175, 203, 238, 247
Psp6I CCWGG 1 cut(s) 33
PspGI CCWGG 1 cut(s) 33
PspN4I GGNNCC 3 cut(s) 141, 168, 316
PsyI GACNNNGTC 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
SalI GTCGAC 1 cut(s) 44
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 4 cut(s) 174, 202, 237, 246
Sau3AI GATC 2 cut(s) 90, 134
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 1 cut(s) 276
SetI ASST 7 cut(s) 25, 30, 36, 53, 77, 175, 344
Sfr274I CTCGAG 1 cut(s) 254
SlaI CTCGAG 1 cut(s) 254
SmlI CTYRAG 2 cut(s) 254, 293
SmoI CTYRAG 2 cut(s) 254, 293
Sse9I AATT 2 cut(s) 240, 249
SsiI CCGC 3 cut(s) 54, 156, 201
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 30
TaqI TCGA 2 cut(s) 45, 255
TasI AATT 2 cut(s) 240, 249
TauI GCSGC 1 cut(s) 204
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TseFI GTSAC 1 cut(s) 279
TseI GCWGC 3 cut(s) 173, 236, 245
Tsp45I GTSAC 1 cut(s) 279
TspGWI ACGGA 1 cut(s) 259
Tth111I GACNNNGTC 1 cut(s) 41
XhoI CTCGAG 1 cut(s) 254
XmiI GTMKAC 2 cut(s) 45, 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.