pycom12g18270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012213
Physical Location & Seq
Forward (+)
20460281 .. 20461316
1036 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 450 bp
ATGAGGCGGGTGAATCTTTTGTGTCAGCTCATAAGCTATCCAATTGGGAAGTTGAGCAAAAGCGAAAAAATGTTGGTCGCAGAAAAGAGCCTTATCTTTAGCAGTAGCCGCAACCTCTCACCTTATCTGTTGCTGAAGAGAAAAAATGGCAGGGAATTTGGAAGCACATTCTTATTAGCCAAAAGGGAAGATATGCCTGAAGATTCGAGGAGATCGCCGGATCAACAGTCGGTCTTTCCGTTGAGGATTCGGGTTTCGAACACGGTTCTTGCGAGGGCTGCGGTTGCGGTGTTCGGGTTGGGATTCATCGATGCAGGATACAGTGGAGACTGGTCGAGGATTGGAGCGATTTCAAAAGAGAGTGAGGAGTTGCTGAAGATTTCTGCATTTCTTGTTGTTCCGTTTTGCTTGTTTCTCATATTTTCCTTCTCTAAAGAAGCTGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.76

Weight (kDa)

9.55

Isoelectric Point (pI)

60.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7887 PF25397 85 - 146 7.5e-36 Domain of unknown function (DUF7887)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012213)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30845
fragaria_vesca FvH4_4g09760 FvH4_4g09760
malus_domestica MD04G1182900.v1.1 MD12G1196500.v1.1
prunus_persica Prupe.1G101900_v2.0.a1
pyrus_communis pycom04g16060 pycom12g18270
rosa_chinensis RchiOBHm_Chr4g0405451
rosa_laevigata RLG00000008841
rosa_multiflora Rmu_sc0000575.1_g000021
rosa_roxburghii Rroxscaffold_5G00349920
rosa_rugosa Rorug04G0060300 Rorug04G0060400
rosa_samantha Rh4AG132000 Rh4BG126900 Rh4CG139400 Rh4DG126000
rosa_wichuraiana Rw4G010690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 7, 109, 281, 287
AclWI GGATC 1 cut(s) 228
AcsI RAATTY 1 cut(s) 155
AcuI CTGAAG 3 cut(s) 155, 219, 395
AgsI TTSAA 1 cut(s) 354
AluBI AGCT 3 cut(s) 28, 36, 440
AluI AGCT 3 cut(s) 28, 36, 440
Alw26I GTCTC 1 cut(s) 321
AlwI GGATC 1 cut(s) 228
ApeKI GCWGC 1 cut(s) 278
ApoI RAATTY 1 cut(s) 155
AsuHPI GGTGA 2 cut(s) 22, 111
AsuII TTCGAA 1 cut(s) 257
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 265
BciVI GTATCC 1 cut(s) 311
BcoDI GTCTC 1 cut(s) 321
BfuI GTATCC 1 cut(s) 311
BisI GCNGC 2 cut(s) 109, 279
BlsI GCNGC 2 cut(s) 110, 280
BmsI GCATC 1 cut(s) 301
Bpu10I CCTNAGC 1 cut(s) 441
Bpu14I TTCGAA 1 cut(s) 257
Bsa29I ATCGAT 1 cut(s) 309
Bse1I ACTGG 1 cut(s) 335
BseCI ATCGAT 1 cut(s) 309
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 1 cut(s) 335
BseRI GAGGAG 2 cut(s) 223, 380
BseXI GCAGC 1 cut(s) 265
BshVI ATCGAT 1 cut(s) 309
BsiSI CCGG 1 cut(s) 218
BsmAI GTCTC 1 cut(s) 321
Bsp119I TTCGAA 1 cut(s) 257
Bsp143I GATC 2 cut(s) 212, 220
BspACI CCGC 4 cut(s) 7, 109, 281, 287
BspCNI CTCAG 1 cut(s) 433
BspDI ATCGAT 1 cut(s) 309
BspPI GGATC 1 cut(s) 228
BspT104I TTCGAA 1 cut(s) 257
BsrI ACTGG 1 cut(s) 335
BssMI GATC 2 cut(s) 212, 220
Bst4CI ACNGT 3 cut(s) 228, 265, 323
Bst6I CTCTTC 1 cut(s) 131
BstBI TTCGAA 1 cut(s) 257
BstDEI CTNAG 1 cut(s) 441
BstKTI GATC 2 cut(s) 215, 223
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 2 cut(s) 212, 220
BstMWI GCNNNNNNNGC 3 cut(s) 108, 278, 284
BstV1I GCAGC 1 cut(s) 265
Bsu15I ATCGAT 1 cut(s) 309
BsuI GTATCC 1 cut(s) 311
BsuTUI ATCGAT 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 328
ClaI ATCGAT 1 cut(s) 309
CviJI RGCY 7 cut(s) 28, 36, 90, 108, 179, 278, 440
CviKI_1 RGCY 7 cut(s) 28, 36, 90, 108, 179, 278, 440
DdeI CTNAG 1 cut(s) 441
DpnI GATC 2 cut(s) 214, 222
DpnII GATC 2 cut(s) 212, 220
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco57I CTGAAG 3 cut(s) 155, 219, 395
FaiI YATR 3 cut(s) 32, 194, 419
Fnu4HI GCNGC 2 cut(s) 109, 279
Fsp4HI GCNGC 2 cut(s) 109, 279
GluI GCNGC 2 cut(s) 109, 279
HapII CCGG 1 cut(s) 218
HinfI GANTC 4 cut(s) 13, 203, 247, 303
HpaII CCGG 1 cut(s) 218
HphI GGTGA 2 cut(s) 22, 111
HpyAV CCTTC 1 cut(s) 436
HpyCH4III ACNGT 3 cut(s) 228, 265, 323
HpyCH4V TGCA 2 cut(s) 314, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 278, 284
HpyF3I CTNAG 1 cut(s) 441
Kzo9I GATC 2 cut(s) 212, 220
LmnI GCTCC 1 cut(s) 344
LpnPI CCDG 5 cut(s) 136, 210, 231, 300, 316
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 1 cut(s) 301
MalI GATC 2 cut(s) 214, 222
MboI GATC 2 cut(s) 212, 220
MboII GAAGA 4 cut(s) 148, 200, 212, 388
MfeI CAATTG 1 cut(s) 42
MluCI AATT 2 cut(s) 42, 155
MnlI CCTC 7 cut(s) 125, 201, 237, 267, 330, 358, 436
MspI CCGG 1 cut(s) 218
MunI CAATTG 1 cut(s) 42
MwoI GCNNNNNNNGC 3 cut(s) 108, 278, 284
NdeII GATC 2 cut(s) 212, 220
NspV TTCGAA 1 cut(s) 257
PcsI WCGNNNNNNNCGW 2 cut(s) 236, 269
PfeI GAWTC 4 cut(s) 13, 203, 247, 303
PkrI GCNGC 2 cut(s) 110, 280
SatI GCNGC 2 cut(s) 109, 279
Sau3AI GATC 2 cut(s) 212, 220
SetI ASST 5 cut(s) 30, 38, 117, 124, 442
SfaNI GCATC 1 cut(s) 301
SfuI TTCGAA 1 cut(s) 257
Sse9I AATT 2 cut(s) 42, 155
SsiI CCGC 4 cut(s) 7, 109, 281, 287
TaaI ACNGT 3 cut(s) 228, 265, 323
TaqI TCGA 4 cut(s) 206, 257, 309, 335
TaqII GACCGA 1 cut(s) 220
TasI AATT 2 cut(s) 42, 155
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 4 cut(s) 13, 203, 247, 303
TscAI CASTG 1 cut(s) 328
TseI GCWGC 1 cut(s) 278
TspDTI ATGAA 1 cut(s) 295
TspGWI ACGGA 2 cut(s) 228, 390
TspRI CASTG 1 cut(s) 328
XapI RAATTY 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.