Rorug04G0060300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000308
Physical Location & Seq
Forward (+)
9439458 .. 9442662
3205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0060300.1

Sequence Viewer

Length: 657 bp
ATGGAAGAAGTGAAACTACTGGGGTTTTGGCCAAGCAATTTTGTTTACAGAGTGATATGGGCTCTAAAACTGAAAGGCGTCGAGTATGATTATATAGAAGAAGACGTCCTTTCCAACAAGAGTGAATTGCTGTTGCAGTACAACCCAGTATACAAGAAGGTCCCGGTGCTTGTTCATCGTGGCAAACCGATTGTTGAGTCATGTGTTATCCTTGAATACATTGAAGAAACTTGGCCTGAGAATCCATTGCTCCCGGAAGATGCTCATGATAAAGCCGTGGCTCGGTTTTGGATGCAGTTTGCGTCTCCCGATCGGAGTCCCACTTTTTATGCATTTTTCCTCTTACCTACTGCTGGGGAAGAGCGCGAAAAAAACATAAAAGATGTACTGGAAACGCTGAGGACATTGGAAGAGCAAGGCCTTGGAGACAAGAAGTTTTTCGGGGGAGACAGCATAGGATTGGTAGACATAGTACATGGATGGCTGGCTTCCTGGTTTGAAATCGTCCAAGAAATGGTAGGGGTGAAATTGCTCGAGCCAAACACTTTGCCTCGATTGCATGCATGGGTTCAGAACTTTAAGGACGCTCCTGTTATCAAAGACAACCTTCCTGACTATAACGAGTTGTTGGCTCATATGACACGGGTTTGGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

25.34

Weight (kDa)

4.97

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 3 - 76 1e-18 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 8 - 81 8.5e-12 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 12 - 77 1.9e-13 Glutathione S-transferase, N-terminal domain
GST_C PF00043 115 - 193 3.8e-06 Glutathione S-transferase, C-terminal domain
DUF7962 PF25907 120 - 196 8.1e-06 Domain of unknown function (DUF7962)
GST_C_2 PF13410 122 - 191 1.3e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000308)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24891 FvH4_4g09031
rosa_multiflora Rmu_co8001542.1_g000001 Rmu_co8074240.1_g000001 Rmu_co8092076.1_g000001 Rmu_co8136936.1_g000001 Rmu_co8303129.1_g000001 Rmu_co8326579.1_g000001 Rmu_co8378475.1_g000001 Rmu_co8409011.1_g000001 Rmu_co8449663.1_g000001 Rmu_co8463085.1_g000001 Rmu_sc0000146.1_g000025 Rmu_sc0000294.1_g000018 Rmu_sc0000299.1_g000003 Rmu_sc0000342.1_g000003 Rmu_sc0000359.1_g000009 Rmu_sc0000431.1_g000022 Rmu_sc0000442.1_g000006 Rmu_sc0000458.1_g000011 Rmu_sc0000560.1_g000013 Rmu_sc0000594.1_g000075 Rmu_sc0000740.1_g000033 Rmu_sc0000839.1_g000002 Rmu_sc0000939.1_g000034 Rmu_sc0000955.1_g000002 Rmu_sc0001069.1_g000045 Rmu_sc0001074.1_g000019 Rmu_sc0001418.1_g000003 Rmu_sc0001484.1_g000006 Rmu_sc0001499.1_g000062 Rmu_sc0001631.1_g000002 Rmu_sc0001639.1_g000021 Rmu_sc0002146.1_g000007 Rmu_sc0002146.1_g000010 Rmu_sc0002260.1_g000065 Rmu_sc0002273.1_g000005 Rmu_sc0002310.1_g000016 Rmu_sc0002414.1_g000051 Rmu_sc0002479.1_g000003 Rmu_sc0002586.1_g000034 Rmu_sc0002586.1_g000035 Rmu_sc0002589.1_g000034 Rmu_sc0003187.1_g000020 Rmu_sc0003444.1_g000018 Rmu_sc0003633.1_g000041 Rmu_sc0003973.1_g000010 Rmu_sc0004209.1_g000004 Rmu_sc0004927.1_g000020 Rmu_sc0004950.1_g000007 Rmu_sc0004952.1_g000012 Rmu_sc0004966.1_g000001 Rmu_sc0006168.1_g000026 Rmu_sc0006325.1_g000003 Rmu_sc0006413.1_g000015 Rmu_sc0007192.1_g000002 Rmu_sc0007390.1_g000004 Rmu_sc0007847.1_g000003 Rmu_sc0008044.1_g000001 Rmu_sc0008101.1_g000008 Rmu_sc0008442.1_g000015 Rmu_sc0008714.1_g000004 Rmu_sc0009057.1_g000009 Rmu_sc0009916.1_g000015 Rmu_sc0010180.1_g000003 Rmu_sc0010560.1_g000005 Rmu_sc0010715.1_g000005 Rmu_sc0011022.1_g000002 Rmu_sc0011509.1_g000006 Rmu_sc0011831.1_g000006 Rmu_sc0012010.1_g000002 Rmu_sc0013320.1_g000007 Rmu_sc0013801.1_g000006 Rmu_sc0013801.1_g000007 Rmu_sc0013887.1_g000002 Rmu_sc0014485.1_g000014 Rmu_sc0018736.1_g000001 Rmu_sc0019355.1_g000001 Rmu_sc0020316.1_g000002 Rmu_sc0021214.1_g000001 Rmu_sc0021375.1_g000001 Rmu_sc0023412.1_g000001 Rmu_sc0024234.1_g000001 Rmu_sc0027415.1_g000001 Rmu_sc0029109.1_g000001 Rmu_sc0031708.1_g000001 Rmu_sc0035307.1_g000001 Rmu_sc0036546.1_g000001 Rmu_sc0037386.1_g000005 Rmu_sc0039204.1_g000001 Rmu_sc0039709.1_g000001 Rmu_sc0039867.1_g000001 Rmu_sc0040008.1_g000002 Rmu_sc0040009.1_g000002 Rmu_sc0041433.1_g000001 Rmu_sc0043076.1_g000001 Rmu_ssc0000024.1_g000022 Rmu_ssc0000065.1_g000003 Rmu_ssc0000100.1_g000036 Rmu_ssc0000171.1_g000015 Rmu_ssc0000190.1_g000018 Rmu_ssc0000226.1_g000017 Rmu_ssc0000244.1_g000039 Rmu_ssc0000419.1_g000008 Rmu_ssc0000451.1_g000014
rosa_roxburghii Rroxscaffold_1G00006150 Rroxscaffold_3G00219360 Rroxscaffold_5G00368320 Rroxscaffold_7G00191310
rosa_rugosa Rorug04G0060300 Rorug04G0115300 Rorug04G0128800
rosa_samantha Rh1AG029600 Rh2BG406100 Rh4DG128300 Rh5DG195500 Rh6AG427300 Rh6CG440900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 108
AccB7I CCANNNNNTGG 1 cut(s) 514
AccI GTMKAC 2 cut(s) 150, 465
AccII CGCG 1 cut(s) 366
AcoI YGGCCR 1 cut(s) 29
AcyI GRCGYC 2 cut(s) 78, 105
AfaI GTAC 3 cut(s) 140, 387, 474
AfiI CCNNNNNNNGG 3 cut(s) 282, 353, 514
AgsI TTSAA 3 cut(s) 215, 224, 500
AjnI CCWGG 1 cut(s) 491
Alw26I GTCTC 3 cut(s) 309, 420, 441
Ama87I CYCGRG 1 cut(s) 533
AoxI GGCC 3 cut(s) 29, 233, 418
Asp700I GAANNNNTTC 1 cut(s) 437
AspLEI GCGC 1 cut(s) 366
AspS9I GGNCC 2 cut(s) 160, 651
AsuC2I CCSGG 2 cut(s) 164, 254
AsuHPI GGTGA 1 cut(s) 535
AvaI CYCGRG 1 cut(s) 533
AvaII GGWCC 2 cut(s) 160, 651
BalI TGGCCA 1 cut(s) 31
BanII GRGCYC 1 cut(s) 64
BarI GAAGNNNNNNTAC 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 108
BbvCI CCTCAGC 1 cut(s) 398
BccI CCATC 1 cut(s) 474
BceAI ACGGC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 493
BcnI CCSGG 2 cut(s) 164, 254
BcoDI GTCTC 3 cut(s) 309, 420, 441
Bme1390I CCNGG 3 cut(s) 164, 254, 493
Bme18I GGWCC 2 cut(s) 160, 651
BmeT110I CYCGRG 1 cut(s) 533
BmgT120I GGNCC 2 cut(s) 160, 651
BmiI GGNNCC 1 cut(s) 162
BmrFI CCNGG 3 cut(s) 164, 254, 493
BmrI ACTGGG 2 cut(s) 29, 140
BmsI GCATC 2 cut(s) 250, 282
BmuI ACTGGG 2 cut(s) 29, 140
BpiI GAAGAC 1 cut(s) 108
Bpu10I CCTNAGC 1 cut(s) 398
BpuMI CCSGG 2 cut(s) 164, 254
BsaHI GRCGYC 2 cut(s) 78, 105
BsaJI CCNNGG 2 cut(s) 276, 421
Bsc4I CCNNNNNNNGG 3 cut(s) 282, 353, 514
Bse1I ACTGG 3 cut(s) 24, 146, 393
Bse3DI GCAATG 1 cut(s) 245
BseBI CCWGG 1 cut(s) 493
BseDI CCNNGG 2 cut(s) 276, 421
BseGI GGATG 2 cut(s) 297, 485
BseLI CCNNNNNNNGG 3 cut(s) 282, 353, 514
BseMI GCAATG 1 cut(s) 245
BseMII CTCAG 2 cut(s) 228, 389
BseNI ACTGG 3 cut(s) 24, 146, 393
BseYI CCCAGC 1 cut(s) 353
Bsh1236I CGCG 1 cut(s) 366
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 3 cut(s) 31, 235, 420
BsiEI CGRYCG 1 cut(s) 313
BsiHKCI CYCGRG 1 cut(s) 533
BsiSI CCGG 2 cut(s) 164, 254
BslFI GGGAC 2 cut(s) 146, 303
BslI CCNNNNNNNGG 3 cut(s) 282, 353, 514
BsmAI GTCTC 3 cut(s) 309, 420, 441
BsmBI CGTCTC 1 cut(s) 309
BsmFI GGGAC 2 cut(s) 146, 303
BsnI GGCC 3 cut(s) 31, 235, 420
BsoBI CYCGRG 1 cut(s) 533
Bsp1286I GDGCHC 1 cut(s) 64
Bsp143I GATC 1 cut(s) 310
BspANI GGCC 3 cut(s) 31, 235, 420
BspCNI CTCAG 2 cut(s) 229, 390
BspFNI CGCG 1 cut(s) 366
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 162
BspQI GCTCTTC 2 cut(s) 354, 405
BsrDI GCAATG 1 cut(s) 245
BsrI ACTGG 3 cut(s) 24, 146, 393
BssECI CCNNGG 2 cut(s) 276, 421
BssMI GATC 1 cut(s) 310
BssNAI GTATAC 1 cut(s) 151
BssNI GRCGYC 2 cut(s) 78, 105
BssT1I CCWWGG 1 cut(s) 421
Bst1107I GTATAC 1 cut(s) 151
Bst2UI CCWGG 1 cut(s) 493
Bst6I CTCTTC 2 cut(s) 354, 405
BstACI GRCGYC 2 cut(s) 78, 105
BstC8I GCNNGC 2 cut(s) 486, 561
BstDEI CTNAG 2 cut(s) 237, 398
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 2 cut(s) 297, 485
BstFNI CGCG 1 cut(s) 366
BstHHI GCGC 1 cut(s) 366
BstKTI GATC 1 cut(s) 313
BstMAI GTCTC 3 cut(s) 309, 420, 441
BstMBI GATC 1 cut(s) 310
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 1 cut(s) 556
BstNI CCWGG 1 cut(s) 493
BstNSI RCATGY 1 cut(s) 563
BstSCI CCNGG 3 cut(s) 162, 252, 491
BstUI CGCG 1 cut(s) 366
BstV2I GAAGAC 1 cut(s) 108
BstZ17I GTATAC 1 cut(s) 151
BsuRI GGCC 3 cut(s) 31, 235, 420
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 2 cut(s) 297, 485
Cac8I GCNNGC 2 cut(s) 486, 561
CciI TCATGA 1 cut(s) 265
CfoI GCGC 1 cut(s) 366
Cfr13I GGNCC 2 cut(s) 160, 651
CseI GACGC 3 cut(s) 67, 291, 593
Csp6I GTAC 3 cut(s) 139, 386, 473
CviAII CATG 5 cut(s) 201, 266, 476, 560, 564
CviQI GTAC 3 cut(s) 139, 386, 473
DdeI CTNAG 2 cut(s) 237, 398
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
EaeI YGGCCR 1 cut(s) 29
Eam1104I CTCTTC 2 cut(s) 354, 405
EarI CTCTTC 2 cut(s) 354, 405
Eco130I CCWWGG 1 cut(s) 421
Eco147I AGGCCT 1 cut(s) 420
Eco24I GRGCYC 1 cut(s) 64
Eco47I GGWCC 2 cut(s) 160, 651
Eco88I CYCGRG 1 cut(s) 533
EcoO109I RGGNCCY 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 491
EcoT14I CCWWGG 1 cut(s) 421
EcoT22I ATGCAT 2 cut(s) 334, 565
EcoT38I GRGCYC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 421
Esp3I CGTCTC 1 cut(s) 309
FaeI CATG 5 cut(s) 204, 269, 479, 563, 567
FalI AAGNNNNNCTT 4 cut(s) 93, 125, 591, 623
FaqI GGGAC 2 cut(s) 146, 303
FatI CATG 5 cut(s) 200, 265, 475, 559, 563
FauNDI CATATG 1 cut(s) 636
FblI GTMKAC 2 cut(s) 150, 465
FokI GGATG 2 cut(s) 304, 492
FriOI GRGCYC 1 cut(s) 64
GlaI GCGC 1 cut(s) 365
GsaI CCCAGC 1 cut(s) 357
HaeIII GGCC 3 cut(s) 31, 235, 420
HapII CCGG 2 cut(s) 164, 254
HgaI GACGC 3 cut(s) 67, 291, 593
HhaI GCGC 1 cut(s) 366
Hin1I GRCGYC 2 cut(s) 78, 105
Hin1II CATG 5 cut(s) 204, 269, 479, 563, 567
Hin6I GCGC 1 cut(s) 364
HinP1I GCGC 1 cut(s) 364
HinfI GANTC 3 cut(s) 197, 241, 316
HpaII CCGG 2 cut(s) 164, 254
HphI GGTGA 1 cut(s) 535
Hpy166II GTNNAC 3 cut(s) 46, 151, 466
Hpy188I TCNGA 2 cut(s) 315, 573
Hpy188III TCNNGA 4 cut(s) 266, 308, 611, 654
Hpy8I GTNNAC 3 cut(s) 46, 151, 466
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 2 cut(s) 151, 617
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 5 cut(s) 136, 295, 332, 559, 563
HpyF10VI GCNNNNNNNGC 1 cut(s) 556
HpyF3I CTNAG 2 cut(s) 237, 398
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 2 cut(s) 78, 105
Hsp92II CATG 5 cut(s) 204, 269, 479, 563, 567
HspAI GCGC 1 cut(s) 364
Kzo9I GATC 1 cut(s) 310
LguI GCTCTTC 2 cut(s) 354, 405
LmnI GCTCC 2 cut(s) 255, 592
LweI GCATC 2 cut(s) 250, 282
MaeII ACGT 1 cut(s) 105
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 7 cut(s) 17, 110, 113, 236, 269, 371, 422
MhlI GDGCHC 1 cut(s) 64
MlsI TGGCCA 1 cut(s) 31
MluCI AATT 3 cut(s) 37, 125, 527
MluNI TGGCCA 1 cut(s) 31
MlyI GAGTC 2 cut(s) 206, 325
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 3 cut(s) 350, 393, 561
Mox20I TGGCCA 1 cut(s) 31
Mph1103I ATGCAT 2 cut(s) 334, 565
MroXI GAANNNNTTC 1 cut(s) 437
MscI TGGCCA 1 cut(s) 31
MseI TTAA 1 cut(s) 579
Msp20I TGGCCA 1 cut(s) 31
MspI CCGG 2 cut(s) 164, 254
MspR9I CCNGG 3 cut(s) 164, 254, 493
MvaI CCWGG 1 cut(s) 493
MvnI CGCG 1 cut(s) 366
MwoI GCNNNNNNNGC 1 cut(s) 556
NciI CCSGG 2 cut(s) 164, 254
NdeI CATATG 1 cut(s) 636
NdeII GATC 1 cut(s) 310
NlaIII CATG 5 cut(s) 204, 269, 479, 563, 567
NlaIV GGNNCC 1 cut(s) 162
NsiI ATGCAT 2 cut(s) 334, 565
NspI RCATGY 1 cut(s) 563
PaeI GCATGC 1 cut(s) 563
PaeR7I CTCGAG 1 cut(s) 533
PagI TCATGA 1 cut(s) 265
PceI AGGCCT 1 cut(s) 420
PciSI GCTCTTC 2 cut(s) 354, 405
PdmI GAANNNNTTC 1 cut(s) 437
PfeI GAWTC 1 cut(s) 241
PflMI CCANNNNNTGG 1 cut(s) 514
PfoI TCCNGGA 1 cut(s) 252
Ple19I CGATCG 1 cut(s) 313
PleI GAGTC 2 cut(s) 205, 324
PpsI GAGTC 2 cut(s) 205, 324
PpuMI RGGWCCY 1 cut(s) 160
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 491
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 1 cut(s) 491
PspN4I GGNNCC 1 cut(s) 162
PspPI GGNCC 2 cut(s) 160, 651
PspPPI RGGWCCY 1 cut(s) 160
PspXI VCTCGAGB 1 cut(s) 533
PvuI CGATCG 1 cut(s) 313
RsaI GTAC 3 cut(s) 140, 387, 474
RsaNI GTAC 3 cut(s) 139, 386, 473
SapI GCTCTTC 2 cut(s) 354, 405
SaqAI TTAA 1 cut(s) 579
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 2 cut(s) 160, 651
SchI GAGTC 2 cut(s) 206, 325
ScrFI CCNGG 3 cut(s) 164, 254, 493
SduI GDGCHC 1 cut(s) 64
SetI ASST 4 cut(s) 108, 162, 349, 609
SfaNI GCATC 2 cut(s) 250, 282
Sfr274I CTCGAG 1 cut(s) 533
SinI GGWCC 2 cut(s) 160, 651
SlaI CTCGAG 1 cut(s) 533
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
SphI GCATGC 1 cut(s) 563
Sse9I AATT 3 cut(s) 37, 125, 527
SseBI AGGCCT 1 cut(s) 420
StuI AGGCCT 1 cut(s) 420
StyD4I CCNGG 3 cut(s) 162, 252, 491
StyI CCWWGG 1 cut(s) 421
TaiI ACGT 1 cut(s) 108
TaqI TCGA 3 cut(s) 81, 534, 553
TasI AATT 3 cut(s) 37, 125, 527
TatI WGTACW 3 cut(s) 138, 385, 472
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TspDTI ATGAA 1 cut(s) 164
Van91I CCANNNNNTGG 1 cut(s) 514
VpaK11BI GGWCC 2 cut(s) 160, 651
XceI RCATGY 1 cut(s) 563
XhoI CTCGAG 1 cut(s) 533
XmiI GTMKAC 2 cut(s) 150, 465
XmnI GAANNNNTTC 1 cut(s) 437
ZraI GACGTC 1 cut(s) 106
Zsp2I ATGCAT 2 cut(s) 334, 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.