Rmu_sc0014485.1_g000014

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0014485.1
OG0000308
Physical Location & Seq
Reverse (-)
44902 .. 45756
855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0014485.1_g000014.1.cds

Sequence Viewer

Length: 855 bp
atggcaattaatgcaatggctgcgcgtctcgctgcttctctcgctcttgcaaaggggaaaaggcccgtggatttgcgcccatccaagggactacgacaacaggaagctttcatggtgggaaagctactcacagtaagagactatactcgccgctcattcatggggatgtttcgatctgcatggagggtgaatggcaccctttgggttgagaaaatcggagaagatgatcgggttcttttctcttttttggatccggctgacaaagttagggtttggaaaggaggaccatggggtttcaacaaagctccgattgcactcgcagactatgacagcgtcggacagatctcggcggtgccattgagggcatcatcctactggatgacgttattgggtgttcctcctgcttttcgatctgaaaggattatacgactgattggcagtaccttggatgatttcaaagagtatgatagaactgctttccggcaaatggtactgtgcataccagttgagatcgagtatgctaaaccattgttgttcaaacagaggttctgggttgaagatgatgttcagtttttggtaaagttcaaacatgataaggtctttgggcgttgtgggctatgtggttttgttacccacgttggcctcccgtgctctggcccagaattggaggaggttgcaggtgtggtggcacaaccatctgcactggcgatactgtgtgatgagccatggaagagttctaatgcacatagggtttcggcgactgtcccaaccctaattttcaaggcaaaactacctagcaaagttccacagttctgtctccctccagtaatactgtactactatcagttccactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

284

Amino Acids

32.01

Weight (kDa)

9.43

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000308)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24891 FvH4_4g09031
rosa_multiflora Rmu_co8001542.1_g000001 Rmu_co8074240.1_g000001 Rmu_co8092076.1_g000001 Rmu_co8136936.1_g000001 Rmu_co8303129.1_g000001 Rmu_co8326579.1_g000001 Rmu_co8378475.1_g000001 Rmu_co8409011.1_g000001 Rmu_co8449663.1_g000001 Rmu_co8463085.1_g000001 Rmu_sc0000146.1_g000025 Rmu_sc0000294.1_g000018 Rmu_sc0000299.1_g000003 Rmu_sc0000342.1_g000003 Rmu_sc0000359.1_g000009 Rmu_sc0000431.1_g000022 Rmu_sc0000442.1_g000006 Rmu_sc0000458.1_g000011 Rmu_sc0000560.1_g000013 Rmu_sc0000594.1_g000075 Rmu_sc0000740.1_g000033 Rmu_sc0000839.1_g000002 Rmu_sc0000939.1_g000034 Rmu_sc0000955.1_g000002 Rmu_sc0001069.1_g000045 Rmu_sc0001074.1_g000019 Rmu_sc0001418.1_g000003 Rmu_sc0001484.1_g000006 Rmu_sc0001499.1_g000062 Rmu_sc0001631.1_g000002 Rmu_sc0001639.1_g000021 Rmu_sc0002146.1_g000007 Rmu_sc0002146.1_g000010 Rmu_sc0002260.1_g000065 Rmu_sc0002273.1_g000005 Rmu_sc0002310.1_g000016 Rmu_sc0002414.1_g000051 Rmu_sc0002479.1_g000003 Rmu_sc0002586.1_g000034 Rmu_sc0002586.1_g000035 Rmu_sc0002589.1_g000034 Rmu_sc0003187.1_g000020 Rmu_sc0003444.1_g000018 Rmu_sc0003633.1_g000041 Rmu_sc0003973.1_g000010 Rmu_sc0004209.1_g000004 Rmu_sc0004927.1_g000020 Rmu_sc0004950.1_g000007 Rmu_sc0004952.1_g000012 Rmu_sc0004966.1_g000001 Rmu_sc0006168.1_g000026 Rmu_sc0006325.1_g000003 Rmu_sc0006413.1_g000015 Rmu_sc0007192.1_g000002 Rmu_sc0007390.1_g000004 Rmu_sc0007847.1_g000003 Rmu_sc0008044.1_g000001 Rmu_sc0008101.1_g000008 Rmu_sc0008442.1_g000015 Rmu_sc0008714.1_g000004 Rmu_sc0009057.1_g000009 Rmu_sc0009916.1_g000015 Rmu_sc0010180.1_g000003 Rmu_sc0010560.1_g000005 Rmu_sc0010715.1_g000005 Rmu_sc0011022.1_g000002 Rmu_sc0011509.1_g000006 Rmu_sc0011831.1_g000006 Rmu_sc0012010.1_g000002 Rmu_sc0013320.1_g000007 Rmu_sc0013801.1_g000006 Rmu_sc0013801.1_g000007 Rmu_sc0013887.1_g000002 Rmu_sc0014485.1_g000014 Rmu_sc0018736.1_g000001 Rmu_sc0019355.1_g000001 Rmu_sc0020316.1_g000002 Rmu_sc0021214.1_g000001 Rmu_sc0021375.1_g000001 Rmu_sc0023412.1_g000001 Rmu_sc0024234.1_g000001 Rmu_sc0027415.1_g000001 Rmu_sc0029109.1_g000001 Rmu_sc0031708.1_g000001 Rmu_sc0035307.1_g000001 Rmu_sc0036546.1_g000001 Rmu_sc0037386.1_g000005 Rmu_sc0039204.1_g000001 Rmu_sc0039709.1_g000001 Rmu_sc0039867.1_g000001 Rmu_sc0040008.1_g000002 Rmu_sc0040009.1_g000002 Rmu_sc0041433.1_g000001 Rmu_sc0043076.1_g000001 Rmu_ssc0000024.1_g000022 Rmu_ssc0000065.1_g000003 Rmu_ssc0000100.1_g000036 Rmu_ssc0000171.1_g000015 Rmu_ssc0000190.1_g000018 Rmu_ssc0000226.1_g000017 Rmu_ssc0000244.1_g000039 Rmu_ssc0000419.1_g000008 Rmu_ssc0000451.1_g000014
rosa_roxburghii Rroxscaffold_1G00006150 Rroxscaffold_3G00219360 Rroxscaffold_5G00368320 Rroxscaffold_7G00191310
rosa_rugosa Rorug04G0060300 Rorug04G0115300 Rorug04G0128800
rosa_samantha Rh1AG029600 Rh2BG406100 Rh4DG128300 Rh5DG195500 Rh6AG427300 Rh6CG440900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 668
Acc36I ACCTGC 1 cut(s) 668
AccB1I GGYRCC 2 cut(s) 194, 352
AccBSI CCGCTC 1 cut(s) 153
AccII CGCG 1 cut(s) 25
AciI CCGC 2 cut(s) 151, 350
AclWI GGATC 2 cut(s) 245, 258
AfaI GTAC 3 cut(s) 442, 492, 836
AfiI CCNNNNNNNGG 5 cut(s) 85, 86, 487, 653, 664
AgsI TTSAA 6 cut(s) 298, 457, 538, 557, 586, 781
AluBI AGCT 3 cut(s) 107, 124, 305
AluI AGCT 3 cut(s) 107, 124, 305
Alw21I GWGCWC 1 cut(s) 653
Alw26I GTCTC 3 cut(s) 32, 132, 821
AlwI GGATC 2 cut(s) 245, 258
AoxI GGCC 3 cut(s) 62, 640, 655
ApeKI GCWGC 2 cut(s) 20, 32
AseI ATTAAT 1 cut(s) 9
AspLEI GCGC 2 cut(s) 25, 78
AspS9I GGNCC 3 cut(s) 63, 284, 656
AsuHPI GGTGA 1 cut(s) 199
AvaII GGWCC 1 cut(s) 284
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 2 cut(s) 194, 352
Bbv12I GWGCWC 1 cut(s) 653
BbvI GCAGC 2 cut(s) 7, 19
BccI CCATC 2 cut(s) 88, 703
BcoDI GTCTC 3 cut(s) 32, 132, 821
BfaI CTAG 1 cut(s) 795
BfuAI ACCTGC 1 cut(s) 668
BglII AGATCT 1 cut(s) 342
BisI GCNGC 3 cut(s) 21, 33, 151
BlsI GCNGC 3 cut(s) 22, 34, 152
Bme18I GGWCC 1 cut(s) 284
BmgT120I GGNCC 3 cut(s) 63, 284, 656
BmiI GGNNCC 3 cut(s) 196, 252, 354
BmsI GCATC 1 cut(s) 374
BpmI CTGGAG 1 cut(s) 807
BsaJI CCNNGG 5 cut(s) 66, 84, 287, 444, 725
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 86, 487, 653, 664
Bse1I ACTGG 4 cut(s) 380, 503, 708, 824
Bse3DI GCAATG 1 cut(s) 21
BseDI CCNNGG 5 cut(s) 66, 84, 287, 444, 725
BseGI GGATG 5 cut(s) 80, 171, 368, 384, 454
BseLI CCNNNNNNNGG 5 cut(s) 85, 86, 487, 653, 664
BseMI GCAATG 1 cut(s) 21
BseNI ACTGG 4 cut(s) 380, 503, 708, 824
BseRI GAGGAG 1 cut(s) 683
BseXI GCAGC 2 cut(s) 7, 19
BsgI GTGCAG 1 cut(s) 684
Bsh1236I CGCG 1 cut(s) 25
BshFI GGCC 3 cut(s) 64, 642, 657
BshNI GGYRCC 2 cut(s) 194, 352
BsiHKAI GWGCWC 1 cut(s) 653
BsiSI CCGG 2 cut(s) 254, 481
BslFI GGGAC 2 cut(s) 102, 749
BslI CCNNNNNNNGG 5 cut(s) 85, 86, 487, 653, 664
BsmAI GTCTC 3 cut(s) 32, 132, 821
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 102, 749
BsnI GGCC 3 cut(s) 64, 642, 657
Bsp1286I GDGCHC 1 cut(s) 653
Bsp143I GATC 6 cut(s) 173, 226, 250, 342, 410, 510
Bsp19I CCATGG 2 cut(s) 287, 725
BspACI CCGC 2 cut(s) 151, 350
BspANI GGCC 3 cut(s) 64, 642, 657
BspFNI CGCG 1 cut(s) 25
BspLI GGNNCC 3 cut(s) 196, 252, 354
BspMI ACCTGC 1 cut(s) 668
BspPI GGATC 2 cut(s) 245, 258
BspT107I GGYRCC 2 cut(s) 194, 352
BsrBI CCGCTC 1 cut(s) 153
BsrDI GCAATG 1 cut(s) 21
BsrI ACTGG 4 cut(s) 380, 503, 708, 824
BssECI CCNNGG 5 cut(s) 66, 84, 287, 444, 725
BssMI GATC 6 cut(s) 173, 226, 250, 342, 410, 510
BssT1I CCWWGG 4 cut(s) 84, 287, 444, 725
Bst4CI ACNGT 6 cut(s) 133, 495, 714, 763, 810, 834
Bst6I CTCTTC 1 cut(s) 725
BstAPI GCANNNNNTGC 2 cut(s) 11, 20
BstDSI CCRYGG 3 cut(s) 66, 287, 725
BstF5I GGATG 5 cut(s) 80, 171, 368, 384, 454
BstFNI CGCG 1 cut(s) 25
BstHHI GCGC 2 cut(s) 25, 78
BstKTI GATC 6 cut(s) 176, 229, 253, 345, 413, 513
BstMAI GTCTC 3 cut(s) 32, 132, 821
BstMBI GATC 6 cut(s) 173, 226, 250, 342, 410, 510
BstMWI GCNNNNNNNGC 7 cut(s) 11, 20, 29, 41, 311, 613, 648
BstUI CGCG 1 cut(s) 25
BstV1I GCAGC 2 cut(s) 7, 19
BstX2I RGATCY 2 cut(s) 250, 342
BstYI RGATCY 2 cut(s) 250, 342
BsuRI GGCC 3 cut(s) 64, 642, 657
BtgI CCRYGG 3 cut(s) 66, 287, 725
BtsCI GGATG 5 cut(s) 80, 171, 368, 384, 454
BtsIMutI CAGTG 1 cut(s) 701
BveI ACCTGC 1 cut(s) 668
CfoI GCGC 2 cut(s) 25, 78
Cfr13I GGNCC 3 cut(s) 63, 284, 656
CseI GACGC 2 cut(s) 14, 322
Csp6I GTAC 3 cut(s) 441, 491, 835
CviAII CATG 6 cut(s) 112, 160, 180, 288, 590, 726
CviQI GTAC 3 cut(s) 441, 491, 835
DpnI GATC 6 cut(s) 175, 228, 252, 344, 412, 512
DpnII GATC 6 cut(s) 173, 226, 250, 342, 410, 510
Eam1104I CTCTTC 1 cut(s) 725
EarI CTCTTC 1 cut(s) 725
Eco130I CCWWGG 4 cut(s) 84, 287, 444, 725
Eco47I GGWCC 1 cut(s) 284
EcoT14I CCWWGG 4 cut(s) 84, 287, 444, 725
ErhI CCWWGG 4 cut(s) 84, 287, 444, 725
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 6 cut(s) 115, 163, 183, 291, 593, 729
FaqI GGGAC 2 cut(s) 102, 749
FatI CATG 6 cut(s) 111, 159, 179, 287, 589, 725
Fnu4HI GCNGC 3 cut(s) 21, 33, 151
FokI GGATG 5 cut(s) 67, 178, 355, 391, 461
Fsp4HI GCNGC 3 cut(s) 21, 33, 151
FspBI CTAG 1 cut(s) 795
GlaI GCGC 2 cut(s) 24, 77
GluI GCNGC 3 cut(s) 21, 33, 151
GsuI CTGGAG 1 cut(s) 807
HaeIII GGCC 3 cut(s) 64, 642, 657
HapII CCGG 2 cut(s) 254, 481
HgaI GACGC 2 cut(s) 14, 322
HhaI GCGC 2 cut(s) 25, 78
Hin1II CATG 6 cut(s) 115, 163, 183, 291, 593, 729
Hin6I GCGC 2 cut(s) 23, 76
HinP1I GCGC 2 cut(s) 23, 76
HindIII AAGCTT 1 cut(s) 105
HpaII CCGG 2 cut(s) 254, 481
HphI GGTGA 1 cut(s) 199
Hpy188I TCNGA 4 cut(s) 218, 309, 338, 415
Hpy99I CGWCG 1 cut(s) 338
HpyCH4III ACNGT 6 cut(s) 133, 495, 714, 763, 810, 834
HpyCH4IV ACGT 2 cut(s) 383, 636
HpyCH4V TGCA 8 cut(s) 14, 50, 179, 314, 498, 677, 701, 743
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 20, 29, 41, 311, 613, 648
HpySE526I ACGT 2 cut(s) 383, 636
Hsp92II CATG 6 cut(s) 115, 163, 183, 291, 593, 729
HspAI GCGC 2 cut(s) 23, 76
Kzo9I GATC 6 cut(s) 173, 226, 250, 342, 410, 510
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 2 cut(s) 7, 19
LweI GCATC 1 cut(s) 374
MaeI CTAG 1 cut(s) 795
MaeII ACGT 2 cut(s) 383, 636
MaeIII GTNAC 1 cut(s) 628
MalI GATC 6 cut(s) 175, 228, 252, 344, 412, 512
MbiI CCGCTC 1 cut(s) 153
MboI GATC 6 cut(s) 173, 226, 250, 342, 410, 510
MboII GAAGA 3 cut(s) 233, 569, 742
MflI RGATCY 2 cut(s) 250, 342
MhlI GDGCHC 1 cut(s) 653
MluCI AATT 3 cut(s) 6, 662, 774
MmeI TCCRAC 1 cut(s) 316
MnlI CCTC 9 cut(s) 177, 275, 354, 408, 537, 653, 661, 664, 831
MseI TTAA 1 cut(s) 9
MslI CAYNNNNRTG 1 cut(s) 164
MspI CCGG 2 cut(s) 254, 481
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 7 cut(s) 11, 20, 29, 41, 311, 613, 648
NcoI CCATGG 2 cut(s) 287, 725
NdeII GATC 6 cut(s) 173, 226, 250, 342, 410, 510
NlaIII CATG 6 cut(s) 115, 163, 183, 291, 593, 729
NlaIV GGNNCC 3 cut(s) 196, 252, 354
NmeAIII GCCGAG 1 cut(s) 326
PaqCI CACCTGC 1 cut(s) 668
PflFI GACNNNGTC 1 cut(s) 332
PkrI GCNGC 3 cut(s) 22, 34, 152
PshBI ATTAAT 1 cut(s) 9
PspN4I GGNNCC 3 cut(s) 196, 252, 354
PspPI GGNCC 3 cut(s) 63, 284, 656
PsuI RGATCY 2 cut(s) 250, 342
PsyI GACNNNGTC 1 cut(s) 332
RsaI GTAC 3 cut(s) 442, 492, 836
RsaNI GTAC 3 cut(s) 441, 491, 835
RseI CAYNNNNRTG 1 cut(s) 164
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 3 cut(s) 21, 33, 151
Sau3AI GATC 6 cut(s) 173, 226, 250, 342, 410, 510
Sau96I GGNCC 3 cut(s) 63, 284, 656
SduI GDGCHC 1 cut(s) 653
SfaNI GCATC 1 cut(s) 374
SinI GGWCC 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 164
Sse9I AATT 3 cut(s) 6, 662, 774
SsiI CCGC 2 cut(s) 151, 350
SspMI CTAG 1 cut(s) 795
StyI CCWWGG 4 cut(s) 84, 287, 444, 725
TaaI ACNGT 6 cut(s) 133, 495, 714, 763, 810, 834
TaiI ACGT 2 cut(s) 386, 639
TaqI TCGA 3 cut(s) 172, 409, 513
TasI AATT 3 cut(s) 6, 662, 774
TatI WGTACW 1 cut(s) 834
TauI GCSGC 1 cut(s) 153
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 1 cut(s) 708
TseI GCWGC 2 cut(s) 20, 32
TspDTI ATGAA 2 cut(s) 100, 148
TspRI CASTG 1 cut(s) 708
Tth111I GACNNNGTC 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 284
VspI ATTAAT 1 cut(s) 9
XspI CTAG 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.