Rmu_sc0036546.1_g000001

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0036546.1
OG0000308
Physical Location & Seq
Reverse (-)
2650 .. 3739
1090 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0036546.1_g000001.1.cds

Sequence Viewer

Length: 1008 bp
ctgtttcgatctgcatggagaattaatggcagattgaatgttgaagaactcggagaagatgatagggtcttgttcacttttacagatccttcggatgtgaatagggtttggaaaggaggaccatcgggatataacagggctcctatagctttagcaccgtatgacggtgtcagtccggcaagggagatcccactgaagaaatcatcatattggatgacactgcaaggagtgccaccggccttcagatctgaacggattatgcgactgattgggagtaccctaggcgaattcatggaatttgatcgccccgcctttcggacccgagttttgcgcatcagggttgagatagattatgctaaacctctcctgtttcgtcgacgtttcagggtggaagatgaagtccaattcatggtgcagtttcgatatgacaagctgtttggtaggtgtggcacctgcggcctagttacccaagttggcctcccttgttcagggctggatctggaagaagaccgtgacatacctgttggcacggtggaacaacccatggctttagccgtgttacggggacaggaaaaccctaatgtcaatagggtaacaataaacaatcaaactctgattttcaaggctcaaatacctaatagtatacccgaagttattctacctcaatctgtgatggccatgcgtcaccatcgaagacaagtgcagattagagaattgagtgaaccaagactcaatgaagatctaatcacaggacaacgccgtatgactatgggcttgttggagggacccccgaacaacaatgatcaacctaagagaaagagaggacgtcctgttggaagtgtgaacaagctgccatatggaacaacaggaaaaggaagaaaaaaggatggaaaacaagaaaagacaaagagcaaaaagaccaacctgatgccgaggttgttagctgctgatgagcctgaaacaagcactaactctaaccctaaggggaaagagcaagtacttgcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

335

Amino Acids

38.08

Weight (kDa)

9.96

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000308)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24891 FvH4_4g09031
rosa_multiflora Rmu_co8001542.1_g000001 Rmu_co8074240.1_g000001 Rmu_co8092076.1_g000001 Rmu_co8136936.1_g000001 Rmu_co8303129.1_g000001 Rmu_co8326579.1_g000001 Rmu_co8378475.1_g000001 Rmu_co8409011.1_g000001 Rmu_co8449663.1_g000001 Rmu_co8463085.1_g000001 Rmu_sc0000146.1_g000025 Rmu_sc0000294.1_g000018 Rmu_sc0000299.1_g000003 Rmu_sc0000342.1_g000003 Rmu_sc0000359.1_g000009 Rmu_sc0000431.1_g000022 Rmu_sc0000442.1_g000006 Rmu_sc0000458.1_g000011 Rmu_sc0000560.1_g000013 Rmu_sc0000594.1_g000075 Rmu_sc0000740.1_g000033 Rmu_sc0000839.1_g000002 Rmu_sc0000939.1_g000034 Rmu_sc0000955.1_g000002 Rmu_sc0001069.1_g000045 Rmu_sc0001074.1_g000019 Rmu_sc0001418.1_g000003 Rmu_sc0001484.1_g000006 Rmu_sc0001499.1_g000062 Rmu_sc0001631.1_g000002 Rmu_sc0001639.1_g000021 Rmu_sc0002146.1_g000007 Rmu_sc0002146.1_g000010 Rmu_sc0002260.1_g000065 Rmu_sc0002273.1_g000005 Rmu_sc0002310.1_g000016 Rmu_sc0002414.1_g000051 Rmu_sc0002479.1_g000003 Rmu_sc0002586.1_g000034 Rmu_sc0002586.1_g000035 Rmu_sc0002589.1_g000034 Rmu_sc0003187.1_g000020 Rmu_sc0003444.1_g000018 Rmu_sc0003633.1_g000041 Rmu_sc0003973.1_g000010 Rmu_sc0004209.1_g000004 Rmu_sc0004927.1_g000020 Rmu_sc0004950.1_g000007 Rmu_sc0004952.1_g000012 Rmu_sc0004966.1_g000001 Rmu_sc0006168.1_g000026 Rmu_sc0006325.1_g000003 Rmu_sc0006413.1_g000015 Rmu_sc0007192.1_g000002 Rmu_sc0007390.1_g000004 Rmu_sc0007847.1_g000003 Rmu_sc0008044.1_g000001 Rmu_sc0008101.1_g000008 Rmu_sc0008442.1_g000015 Rmu_sc0008714.1_g000004 Rmu_sc0009057.1_g000009 Rmu_sc0009916.1_g000015 Rmu_sc0010180.1_g000003 Rmu_sc0010560.1_g000005 Rmu_sc0010715.1_g000005 Rmu_sc0011022.1_g000002 Rmu_sc0011509.1_g000006 Rmu_sc0011831.1_g000006 Rmu_sc0012010.1_g000002 Rmu_sc0013320.1_g000007 Rmu_sc0013801.1_g000006 Rmu_sc0013801.1_g000007 Rmu_sc0013887.1_g000002 Rmu_sc0014485.1_g000014 Rmu_sc0018736.1_g000001 Rmu_sc0019355.1_g000001 Rmu_sc0020316.1_g000002 Rmu_sc0021214.1_g000001 Rmu_sc0021375.1_g000001 Rmu_sc0023412.1_g000001 Rmu_sc0024234.1_g000001 Rmu_sc0027415.1_g000001 Rmu_sc0029109.1_g000001 Rmu_sc0031708.1_g000001 Rmu_sc0035307.1_g000001 Rmu_sc0036546.1_g000001 Rmu_sc0037386.1_g000005 Rmu_sc0039204.1_g000001 Rmu_sc0039709.1_g000001 Rmu_sc0039867.1_g000001 Rmu_sc0040008.1_g000002 Rmu_sc0040009.1_g000002 Rmu_sc0041433.1_g000001 Rmu_sc0043076.1_g000001 Rmu_ssc0000024.1_g000022 Rmu_ssc0000065.1_g000003 Rmu_ssc0000100.1_g000036 Rmu_ssc0000171.1_g000015 Rmu_ssc0000190.1_g000018 Rmu_ssc0000226.1_g000017 Rmu_ssc0000244.1_g000039 Rmu_ssc0000419.1_g000008 Rmu_ssc0000451.1_g000014
rosa_roxburghii Rroxscaffold_1G00006150 Rroxscaffold_3G00219360 Rroxscaffold_5G00368320 Rroxscaffold_7G00191310
rosa_rugosa Rorug04G0060300 Rorug04G0115300 Rorug04G0128800
rosa_samantha Rh1AG029600 Rh2BG406100 Rh4DG128300 Rh5DG195500 Rh6AG427300 Rh6CG440900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 461
AatII GACGTC 1 cut(s) 829
Acc16I TGCGCA 1 cut(s) 332
Acc36I ACCTGC 1 cut(s) 461
AccB1I GGYRCC 1 cut(s) 449
AccB7I CCANNNNNTGG 1 cut(s) 409
AccI GTMKAC 2 cut(s) 376, 643
AciI CCGC 2 cut(s) 309, 456
AclWI GGATC 3 cut(s) 80, 181, 504
AcoI YGGCCR 1 cut(s) 675
AcsI RAATTY 2 cut(s) 287, 296
AcuI CTGAAG 2 cut(s) 215, 226
AcyI GRCGYC 1 cut(s) 826
AfaI GTAC 2 cut(s) 277, 999
AfiI CCNNNNNNNGG 5 cut(s) 164, 315, 409, 488, 561
AgsI TTSAA 3 cut(s) 37, 44, 622
AluBI AGCT 4 cut(s) 149, 433, 850, 944
AluI AGCT 4 cut(s) 149, 433, 850, 944
AlwI GGATC 3 cut(s) 80, 181, 504
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 4 cut(s) 237, 457, 475, 675
ApeKI GCWGC 2 cut(s) 850, 944
ApoI RAATTY 2 cut(s) 287, 296
ArsI GACNNNNNNTTYG 4 cut(s) 310, 342, 419, 451
AseI ATTAAT 1 cut(s) 24
Asp700I GAANNNNTTC 1 cut(s) 654
AspA2I CCTAGG 1 cut(s) 280
AspLEI GCGC 1 cut(s) 333
AspS9I GGNCC 3 cut(s) 119, 318, 785
AsuHPI GGTGA 1 cut(s) 677
AvaI CYCGRG 1 cut(s) 321
AvaII GGWCC 3 cut(s) 119, 318, 785
AvrII CCTAGG 1 cut(s) 280
AxyI CCTNAGG 1 cut(s) 981
BalI TGGCCA 1 cut(s) 677
BanI GGYRCC 1 cut(s) 449
BanII GRGCYC 1 cut(s) 142
BarI GAAGNNNNNNTAC 2 cut(s) 642, 674
BbsI GAAGAC 2 cut(s) 513, 700
BbvI GCAGC 2 cut(s) 837, 931
BccI CCATC 4 cut(s) 130, 667, 696, 881
BceAI ACGGC 2 cut(s) 539, 744
BclI TGATCA 1 cut(s) 802
BfaI CTAG 2 cut(s) 281, 461
BfmI CTRYAG 1 cut(s) 144
BfuAI ACCTGC 1 cut(s) 461
BglII AGATCT 2 cut(s) 245, 739
BisI GCNGC 3 cut(s) 457, 851, 945
BlnI CCTAGG 1 cut(s) 280
BlsI GCNGC 3 cut(s) 458, 852, 946
BmcAI AGTACT 1 cut(s) 999
Bme18I GGWCC 3 cut(s) 119, 318, 785
BmeT110I CYCGRG 1 cut(s) 321
BmgT120I GGNCC 3 cut(s) 119, 318, 785
BmiI GGNNCC 5 cut(s) 141, 320, 451, 786, 787
BmsI GCATC 2 cut(s) 342, 918
BpiI GAAGAC 2 cut(s) 513, 700
BsaHI GRCGYC 1 cut(s) 826
BsaJI CCNNGG 3 cut(s) 280, 543, 932
Bsc4I CCNNNNNNNGG 5 cut(s) 164, 315, 409, 488, 561
Bse118I RCCGGY 1 cut(s) 235
Bse21I CCTNAGG 1 cut(s) 981
BseDI CCNNGG 3 cut(s) 280, 543, 932
BseGI GGATG 3 cut(s) 100, 219, 892
BseLI CCNNNNNNNGG 5 cut(s) 164, 315, 409, 488, 561
BseXI GCAGC 2 cut(s) 837, 931
BsgI GTGCAG 2 cut(s) 434, 722
BshFI GGCC 4 cut(s) 239, 459, 477, 677
BshNI GGYRCC 1 cut(s) 449
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 2 cut(s) 176, 236
BslFI GGGAC 2 cut(s) 579, 798
BslI CCNNNNNNNGG 5 cut(s) 164, 315, 409, 488, 561
BsmFI GGGAC 2 cut(s) 579, 798
BsnI GGCC 4 cut(s) 239, 459, 477, 677
BsoBI CYCGRG 1 cut(s) 321
Bsp1286I GDGCHC 1 cut(s) 142
Bsp143I GATC 8 cut(s) 8, 85, 186, 245, 301, 496, 739, 802
Bsp19I CCATGG 1 cut(s) 543
BspACI CCGC 2 cut(s) 309, 456
BspANI GGCC 4 cut(s) 239, 459, 477, 677
BspLI GGNNCC 5 cut(s) 141, 320, 451, 786, 787
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 3 cut(s) 80, 181, 504
BspT107I GGYRCC 1 cut(s) 449
BsrFI RCCGGY 1 cut(s) 235
BssAI RCCGGY 1 cut(s) 235
BssECI CCNNGG 3 cut(s) 280, 543, 932
BssMI GATC 8 cut(s) 8, 85, 186, 245, 301, 496, 739, 802
BssNAI GTATAC 1 cut(s) 644
BssNI GRCGYC 1 cut(s) 826
BssT1I CCWWGG 2 cut(s) 280, 543
Bst1107I GTATAC 1 cut(s) 644
Bst4CI ACNGT 4 cut(s) 159, 167, 512, 532
BstACI GRCGYC 1 cut(s) 826
BstAPI GCANNNNNTGC 1 cut(s) 229
BstDEI CTNAG 2 cut(s) 810, 981
BstDSI CCRYGG 1 cut(s) 543
BstENI CCTNNNNNAGG 1 cut(s) 486
BstF5I GGATG 3 cut(s) 100, 219, 892
BstHHI GCGC 1 cut(s) 333
BstKTI GATC 8 cut(s) 11, 88, 189, 248, 304, 499, 742, 805
BstMBI GATC 8 cut(s) 8, 85, 186, 245, 301, 496, 739, 802
BstMWI GCNNNNNNNGC 3 cut(s) 146, 229, 456
BstSFI CTRYAG 1 cut(s) 144
BstV1I GCAGC 2 cut(s) 837, 931
BstV2I GAAGAC 2 cut(s) 513, 700
BstX2I RGATCY 5 cut(s) 85, 186, 245, 496, 739
BstYI RGATCY 5 cut(s) 85, 186, 245, 496, 739
BstZ17I GTATAC 1 cut(s) 644
Bsu36I CCTNAGG 1 cut(s) 981
BsuRI GGCC 4 cut(s) 239, 459, 477, 677
BtgI CCRYGG 1 cut(s) 543
BtsCI GGATG 3 cut(s) 100, 219, 892
BtsI GCAGTG 1 cut(s) 218
BtsIMutI CAGTG 2 cut(s) 191, 218
BveI ACCTGC 1 cut(s) 461
CfoI GCGC 1 cut(s) 333
Cfr10I RCCGGY 1 cut(s) 235
Cfr13I GGNCC 3 cut(s) 119, 318, 785
CseI GACGC 1 cut(s) 671
Csp6I GTAC 2 cut(s) 276, 998
CviAII CATG 5 cut(s) 15, 292, 409, 544, 679
CviQI GTAC 2 cut(s) 276, 998
DdeI CTNAG 2 cut(s) 810, 981
DpnI GATC 8 cut(s) 10, 87, 188, 247, 303, 498, 741, 804
DpnII GATC 8 cut(s) 8, 85, 186, 245, 301, 496, 739, 802
EaeI YGGCCR 1 cut(s) 675
Eco130I CCWWGG 2 cut(s) 280, 543
Eco24I GRGCYC 1 cut(s) 142
Eco47I GGWCC 3 cut(s) 119, 318, 785
Eco57I CTGAAG 2 cut(s) 215, 226
Eco81I CCTNAGG 1 cut(s) 981
Eco88I CYCGRG 1 cut(s) 321
EcoNI CCTNNNNNAGG 1 cut(s) 486
EcoO109I RGGNCCY 1 cut(s) 785
EcoRI GAATTC 1 cut(s) 287
EcoT14I CCWWGG 2 cut(s) 280, 543
EcoT38I GRGCYC 1 cut(s) 142
ErhI CCWWGG 2 cut(s) 280, 543
FaeI CATG 5 cut(s) 18, 295, 412, 547, 682
FaqI GGGAC 2 cut(s) 579, 798
FatI CATG 5 cut(s) 14, 291, 408, 543, 678
FauI CCCGC 1 cut(s) 316
FauNDI CATATG 1 cut(s) 856
FbaI TGATCA 1 cut(s) 802
FblI GTMKAC 2 cut(s) 376, 643
Fnu4HI GCNGC 3 cut(s) 457, 851, 945
FokI GGATG 3 cut(s) 107, 226, 899
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 3 cut(s) 457, 851, 945
FspBI CTAG 2 cut(s) 281, 461
FspI TGCGCA 1 cut(s) 332
GlaI GCGC 1 cut(s) 332
GluI GCNGC 3 cut(s) 457, 851, 945
HaeIII GGCC 4 cut(s) 239, 459, 477, 677
HapII CCGG 2 cut(s) 176, 236
HgaI GACGC 1 cut(s) 671
HhaI GCGC 1 cut(s) 333
Hin1I GRCGYC 1 cut(s) 826
Hin1II CATG 5 cut(s) 18, 295, 412, 547, 682
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HincII GTYRAC 1 cut(s) 377
HindII GTYRAC 1 cut(s) 377
HinfI GANTC 1 cut(s) 729
HpaII CCGG 2 cut(s) 176, 236
HphI GGTGA 1 cut(s) 677
Hpy166II GTNNAC 5 cut(s) 75, 377, 644, 722, 844
Hpy188I TCNGA 6 cut(s) 53, 94, 245, 250, 318, 615
Hpy188III TCNNGA 2 cut(s) 126, 500
Hpy8I GTNNAC 5 cut(s) 75, 377, 644, 722, 844
Hpy99I CGWCG 2 cut(s) 378, 381
HpyAV CCTTC 2 cut(s) 99, 250
HpyCH4III ACNGT 4 cut(s) 159, 167, 512, 532
HpyCH4IV ACGT 2 cut(s) 379, 826
HpyCH4V TGCA 4 cut(s) 14, 223, 415, 703
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 229, 456
HpyF3I CTNAG 2 cut(s) 810, 981
HpySE526I ACGT 2 cut(s) 379, 826
Hsp92I GRCGYC 1 cut(s) 826
Hsp92II CATG 5 cut(s) 18, 295, 412, 547, 682
HspAI GCGC 1 cut(s) 331
KflI GGGWCCC 1 cut(s) 785
Ksp22I TGATCA 1 cut(s) 802
Kzo9I GATC 8 cut(s) 8, 85, 186, 245, 301, 496, 739, 802
LmnI GCTCC 1 cut(s) 145
Lsp1109I GCAGC 2 cut(s) 837, 931
LweI GCATC 2 cut(s) 342, 918
MaeI CTAG 2 cut(s) 281, 461
MaeII ACGT 2 cut(s) 379, 826
MaeIII GTNAC 5 cut(s) 463, 512, 558, 592, 683
MalI GATC 8 cut(s) 10, 87, 188, 247, 303, 498, 741, 804
MboI GATC 8 cut(s) 8, 85, 186, 245, 301, 496, 739, 802
MboII GAAGA 9 cut(s) 56, 68, 208, 404, 515, 518, 705, 749, 888
MflI RGATCY 5 cut(s) 85, 186, 245, 496, 739
MhlI GDGCHC 1 cut(s) 142
MlsI TGGCCA 1 cut(s) 677
MluCI AATT 5 cut(s) 21, 287, 296, 404, 713
MluNI TGGCCA 1 cut(s) 677
MlyI GAGTC 1 cut(s) 723
MmeI TCCRAC 2 cut(s) 759, 814
MnlI CCTC 7 cut(s) 110, 372, 488, 672, 775, 815, 927
Mox20I TGGCCA 1 cut(s) 677
MroXI GAANNNNTTC 1 cut(s) 654
MscI TGGCCA 1 cut(s) 677
MseI TTAA 2 cut(s) 24, 1006
Msp20I TGGCCA 1 cut(s) 677
MspI CCGG 2 cut(s) 176, 236
MwoI GCNNNNNNNGC 3 cut(s) 146, 229, 456
NcoI CCATGG 1 cut(s) 543
NdeI CATATG 1 cut(s) 856
NdeII GATC 8 cut(s) 8, 85, 186, 245, 301, 496, 739, 802
NlaIII CATG 5 cut(s) 18, 295, 412, 547, 682
NlaIV GGNNCC 5 cut(s) 141, 320, 451, 786, 787
NmeAIII GCCGAG 1 cut(s) 957
NmuCI GTSAC 2 cut(s) 512, 683
NsbI TGCGCA 1 cut(s) 332
PaqCI CACCTGC 1 cut(s) 461
PcsI WCGNNNNNNNCGW 1 cut(s) 259
PdmI GAANNNNTTC 1 cut(s) 654
PflFI GACNNNGTC 1 cut(s) 167
PflMI CCANNNNNTGG 1 cut(s) 409
PkrI GCNGC 3 cut(s) 458, 852, 946
PleI GAGTC 1 cut(s) 723
PpsI GAGTC 1 cut(s) 723
PpuMI RGGWCCY 1 cut(s) 785
PshBI ATTAAT 1 cut(s) 24
Psp5II RGGWCCY 1 cut(s) 785
PspN4I GGNNCC 5 cut(s) 141, 320, 451, 786, 787
PspPI GGNCC 3 cut(s) 119, 318, 785
PspPPI RGGWCCY 1 cut(s) 785
PsuI RGATCY 5 cut(s) 85, 186, 245, 496, 739
PsyI GACNNNGTC 1 cut(s) 167
RsaI GTAC 2 cut(s) 277, 999
RsaNI GTAC 2 cut(s) 276, 998
SalI GTCGAC 1 cut(s) 375
SaqAI TTAA 2 cut(s) 24, 1006
SatI GCNGC 3 cut(s) 457, 851, 945
Sau3AI GATC 8 cut(s) 8, 85, 186, 245, 301, 496, 739, 802
Sau96I GGNCC 3 cut(s) 119, 318, 785
ScaI AGTACT 1 cut(s) 999
SchI GAGTC 1 cut(s) 723
SduI GDGCHC 1 cut(s) 142
SfaNI GCATC 2 cut(s) 342, 918
SfcI CTRYAG 1 cut(s) 144
SgrDI CGTCGACG 1 cut(s) 375
SinI GGWCC 3 cut(s) 119, 318, 785
Sse9I AATT 5 cut(s) 21, 287, 296, 404, 713
SsiI CCGC 2 cut(s) 309, 456
SspMI CTAG 2 cut(s) 281, 461
StyI CCWWGG 2 cut(s) 280, 543
TaaI ACNGT 4 cut(s) 159, 167, 512, 532
TaiI ACGT 2 cut(s) 382, 829
TaqI TCGA 4 cut(s) 7, 376, 421, 691
TasI AATT 5 cut(s) 21, 287, 296, 404, 713
TatI WGTACW 1 cut(s) 997
TauI GCSGC 1 cut(s) 459
Tru1I TTAA 2 cut(s) 24, 1006
Tru9I TTAA 2 cut(s) 24, 1006
TscAI CASTG 2 cut(s) 198, 225
TseFI GTSAC 2 cut(s) 512, 683
TseI GCWGC 2 cut(s) 850, 944
Tsp45I GTSAC 2 cut(s) 512, 683
TspDTI ATGAA 4 cut(s) 280, 397, 411, 750
TspGWI ACGGA 1 cut(s) 268
TspRI CASTG 2 cut(s) 198, 225
Tth111I GACNNNGTC 1 cut(s) 167
Van91I CCANNNNNTGG 1 cut(s) 409
VpaK11BI GGWCC 3 cut(s) 119, 318, 785
VspI ATTAAT 1 cut(s) 24
XagI CCTNNNNNAGG 1 cut(s) 486
XapI RAATTY 2 cut(s) 287, 296
XmaJI CCTAGG 1 cut(s) 280
XmiI GTMKAC 2 cut(s) 376, 643
XmnI GAANNNNTTC 1 cut(s) 654
XspI CTAG 2 cut(s) 281, 461
ZraI GACGTC 1 cut(s) 827
ZrmI AGTACT 1 cut(s) 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.