Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 193 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 71, 286 |
| AciI | CCGC | 1 cut(s) | 324 |
| AcuI | CTGAAG | 1 cut(s) | 122 |
| AcyI | GRCGYC | 1 cut(s) | 75 |
| AfaI | GTAC | 2 cut(s) | 138, 435 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 71, 286, 365, 366 |
| AgsI | TTSAA | 2 cut(s) | 269, 383 |
| AhlI | ACTAGT | 1 cut(s) | 337 |
| AjiI | CACGTC | 1 cut(s) | 423 |
| AjnI | CCWGG | 1 cut(s) | 28 |
| AluBI | AGCT | 2 cut(s) | 47, 310 |
| AluI | AGCT | 2 cut(s) | 47, 310 |
| Alw21I | GWGCWC | 1 cut(s) | 49 |
| Alw26I | GTCTC | 1 cut(s) | 429 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 286 |
| Ama87I | CYCGRG | 1 cut(s) | 198 |
| AoxI | GGCC | 3 cut(s) | 352, 368, 507 |
| ApeKI | GCWGC | 2 cut(s) | 321, 449 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 174, 206, 296, 328 |
| AspLEI | GCGC | 2 cut(s) | 64, 162 |
| AspS9I | GGNCC | 3 cut(s) | 25, 368, 508 |
| AsuII | TTCGAA | 1 cut(s) | 193 |
| AvaI | CYCGRG | 1 cut(s) | 198 |
| AvaII | GGWCC | 1 cut(s) | 25 |
| BanII | GRGCYC | 1 cut(s) | 49 |
| BbsI | GAAGAC | 2 cut(s) | 96, 187 |
| Bbv12I | GWGCWC | 1 cut(s) | 49 |
| BbvI | GCAGC | 2 cut(s) | 308, 461 |
| BccI | CCATC | 1 cut(s) | 65 |
| BciT130I | CCWGG | 1 cut(s) | 30 |
| BciVI | GTATCC | 1 cut(s) | 29 |
| BcoDI | GTCTC | 1 cut(s) | 429 |
| BcuI | ACTAGT | 1 cut(s) | 337 |
| BfaI | CTAG | 3 cut(s) | 59, 83, 338 |
| BfoI | RGCGCY | 1 cut(s) | 65 |
| BfuAI | ACCTGC | 1 cut(s) | 193 |
| BfuI | GTATCC | 1 cut(s) | 29 |
| BisI | GCNGC | 2 cut(s) | 322, 450 |
| BlsI | GCNGC | 2 cut(s) | 323, 451 |
| Bme1390I | CCNGG | 1 cut(s) | 30 |
| Bme18I | GGWCC | 1 cut(s) | 25 |
| BmeT110I | CYCGRG | 1 cut(s) | 198 |
| BmgBI | CACGTC | 1 cut(s) | 423 |
| BmgT120I | GGNCC | 3 cut(s) | 25, 368, 508 |
| BmiI | GGNNCC | 1 cut(s) | 27 |
| BmrFI | CCNGG | 1 cut(s) | 30 |
| BmsI | GCATC | 2 cut(s) | 171, 557 |
| BpiI | GAAGAC | 2 cut(s) | 96, 187 |
| Bpu10I | CCTNAGC | 1 cut(s) | 364 |
| Bpu14I | TTCGAA | 1 cut(s) | 193 |
| BsaHI | GRCGYC | 1 cut(s) | 75 |
| BsaJI | CCNNGG | 3 cut(s) | 28, 29, 518 |
| BsaWI | WCCGGW | 1 cut(s) | 297 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 71, 286, 365, 366 |
| Bse1I | ACTGG | 3 cut(s) | 122, 381, 402 |
| BseBI | CCWGG | 1 cut(s) | 30 |
| BseDI | CCNNGG | 3 cut(s) | 28, 29, 518 |
| BseGI | GGATG | 2 cut(s) | 110, 126 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 71, 286, 365, 366 |
| BseMII | CTCAG | 2 cut(s) | 147, 378 |
| BseNI | ACTGG | 3 cut(s) | 122, 381, 402 |
| BseRI | GAGGAG | 1 cut(s) | 530 |
| BseXI | GCAGC | 2 cut(s) | 308, 461 |
| BshFI | GGCC | 3 cut(s) | 354, 370, 509 |
| BsiHKAI | GWGCWC | 1 cut(s) | 49 |
| BsiHKCI | CYCGRG | 1 cut(s) | 198 |
| BsiSI | CCGG | 1 cut(s) | 298 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 71, 286, 365, 366 |
| BsmAI | GTCTC | 1 cut(s) | 429 |
| BsmBI | CGTCTC | 1 cut(s) | 429 |
| BsnI | GGCC | 3 cut(s) | 354, 370, 509 |
| BsoBI | CYCGRG | 1 cut(s) | 198 |
| Bsp119I | TTCGAA | 1 cut(s) | 193 |
| Bsp1286I | GDGCHC | 1 cut(s) | 49 |
| Bsp143I | GATC | 1 cut(s) | 174 |
| BspACI | CCGC | 1 cut(s) | 324 |
| BspANI | GGCC | 3 cut(s) | 354, 370, 509 |
| BspCNI | CTCAG | 2 cut(s) | 148, 377 |
| BspLI | GGNNCC | 1 cut(s) | 27 |
| BspMI | ACCTGC | 1 cut(s) | 193 |
| BspT104I | TTCGAA | 1 cut(s) | 193 |
| BsrI | ACTGG | 3 cut(s) | 122, 381, 402 |
| BssECI | CCNNGG | 3 cut(s) | 28, 29, 518 |
| BssMI | GATC | 1 cut(s) | 174 |
| BssNI | GRCGYC | 1 cut(s) | 75 |
| BssT1I | CCWWGG | 1 cut(s) | 518 |
| Bst2UI | CCWGG | 1 cut(s) | 30 |
| Bst4CI | ACNGT | 2 cut(s) | 141, 433 |
| BstACI | GRCGYC | 1 cut(s) | 75 |
| BstBI | TTCGAA | 1 cut(s) | 193 |
| BstDEI | CTNAG | 2 cut(s) | 156, 364 |
| BstF5I | GGATG | 2 cut(s) | 110, 126 |
| BstH2I | RGCGCY | 1 cut(s) | 65 |
| BstHHI | GCGC | 2 cut(s) | 64, 162 |
| BstKTI | GATC | 1 cut(s) | 177 |
| BstMAI | GTCTC | 1 cut(s) | 429 |
| BstMBI | GATC | 1 cut(s) | 174 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 53, 166, 360 |
| BstNI | CCWGG | 1 cut(s) | 30 |
| BstNSI | RCATGY | 1 cut(s) | 97 |
| BstSCI | CCNGG | 1 cut(s) | 28 |
| BstV1I | GCAGC | 2 cut(s) | 308, 461 |
| BstV2I | GAAGAC | 2 cut(s) | 96, 187 |
| BsuI | GTATCC | 1 cut(s) | 29 |
| BsuRI | GGCC | 3 cut(s) | 354, 370, 509 |
| BtrI | CACGTC | 1 cut(s) | 423 |
| BtsCI | GGATG | 2 cut(s) | 110, 126 |
| BtsI | GCAGTG | 1 cut(s) | 125 |
| BtsIMutI | CAGTG | 3 cut(s) | 125, 395, 558 |
| BveI | ACCTGC | 1 cut(s) | 193 |
| CaiI | CAGNNNCTG | 1 cut(s) | 286 |
| CfoI | GCGC | 2 cut(s) | 64, 162 |
| Cfr13I | GGNCC | 3 cut(s) | 25, 368, 508 |
| CseI | GACGC | 3 cut(s) | 64, 401, 554 |
| Csp6I | GTAC | 2 cut(s) | 137, 434 |
| CviAII | CATG | 5 cut(s) | 94, 166, 347, 446, 562 |
| CviJI | RGCY | 9 cut(s) | 47, 56, 238, 256, 310, 354, 370, 509, 560 |
| CviKI_1 | RGCY | 9 cut(s) | 47, 56, 238, 256, 310, 354, 370, 509, 560 |
| CviQI | GTAC | 2 cut(s) | 137, 434 |
| DdeI | CTNAG | 2 cut(s) | 156, 364 |
| DpnI | GATC | 1 cut(s) | 176 |
| DpnII | GATC | 1 cut(s) | 174 |
| Ecl136II | GAGCTC | 1 cut(s) | 47 |
| Eco130I | CCWWGG | 1 cut(s) | 518 |
| Eco24I | GRGCYC | 1 cut(s) | 49 |
| Eco47I | GGWCC | 1 cut(s) | 25 |
| Eco53kI | GAGCTC | 1 cut(s) | 47 |
| Eco57I | CTGAAG | 1 cut(s) | 122 |
| Eco88I | CYCGRG | 1 cut(s) | 198 |
| EcoICRI | GAGCTC | 1 cut(s) | 47 |
| EcoO109I | RGGNCCY | 1 cut(s) | 25 |
| EcoRII | CCWGG | 1 cut(s) | 28 |
| EcoT14I | CCWWGG | 1 cut(s) | 518 |
| EcoT38I | GRGCYC | 1 cut(s) | 49 |
| ErhI | CCWWGG | 1 cut(s) | 518 |
| Esp3I | CGTCTC | 1 cut(s) | 429 |
| FaeI | CATG | 5 cut(s) | 97, 169, 350, 449, 565 |
| FatI | CATG | 5 cut(s) | 93, 165, 346, 445, 561 |
| FauNDI | CATATG | 1 cut(s) | 67 |
| Fnu4HI | GCNGC | 2 cut(s) | 322, 450 |
| FokI | GGATG | 2 cut(s) | 97, 133 |
| FriOI | GRGCYC | 1 cut(s) | 49 |
| Fsp4HI | GCNGC | 2 cut(s) | 322, 450 |
| FspBI | CTAG | 3 cut(s) | 59, 83, 338 |
| GlaI | GCGC | 2 cut(s) | 63, 161 |
| GluI | GCNGC | 2 cut(s) | 322, 450 |
| HaeII | RGCGCY | 1 cut(s) | 65 |
| HaeIII | GGCC | 3 cut(s) | 354, 370, 509 |
| HapII | CCGG | 1 cut(s) | 298 |
| HgaI | GACGC | 3 cut(s) | 64, 401, 554 |
| HhaI | GCGC | 2 cut(s) | 64, 162 |
| Hin1I | GRCGYC | 1 cut(s) | 75 |
| Hin1II | CATG | 5 cut(s) | 97, 169, 350, 449, 565 |
| Hin6I | GCGC | 2 cut(s) | 62, 160 |
| HinP1I | GCGC | 2 cut(s) | 62, 160 |
| HinfI | GANTC | 3 cut(s) | 201, 216, 283 |
| HpaII | CCGG | 1 cut(s) | 298 |
| Hpy166II | GTNNAC | 2 cut(s) | 6, 335 |
| Hpy188I | TCNGA | 6 cut(s) | 51, 157, 174, 179, 215, 429 |
| Hpy188III | TCNNGA | 2 cut(s) | 220, 280 |
| Hpy8I | GTNNAC | 2 cut(s) | 6, 335 |
| Hpy99I | CGWCG | 3 cut(s) | 145, 255, 570 |
| HpyAV | CCTTC | 1 cut(s) | 365 |
| HpyCH4III | ACNGT | 2 cut(s) | 141, 433 |
| HpyCH4IV | ACGT | 1 cut(s) | 422 |
| HpyCH4V | TGCA | 5 cut(s) | 130, 188, 292, 449, 548 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 53, 166, 360 |
| HpyF3I | CTNAG | 2 cut(s) | 156, 364 |
| HpySE526I | ACGT | 1 cut(s) | 422 |
| Hsp92I | GRCGYC | 1 cut(s) | 75 |
| Hsp92II | CATG | 5 cut(s) | 97, 169, 350, 449, 565 |
| HspAI | GCGC | 2 cut(s) | 62, 160 |
| Kzo9I | GATC | 1 cut(s) | 174 |
| LmnI | GCTCC | 2 cut(s) | 52, 522 |
| Lsp1109I | GCAGC | 2 cut(s) | 308, 461 |
| LweI | GCATC | 2 cut(s) | 171, 557 |
| MaeI | CTAG | 3 cut(s) | 59, 83, 338 |
| MaeII | ACGT | 1 cut(s) | 422 |
| MaeIII | GTNAC | 4 cut(s) | 76, 340, 389, 569 |
| MalI | GATC | 1 cut(s) | 176 |
| MboI | GATC | 1 cut(s) | 174 |
| MboII | GAAGA | 5 cut(s) | 101, 115, 192, 281, 395 |
| MhlI | GDGCHC | 1 cut(s) | 49 |
| MluCI | AATT | 1 cut(s) | 293 |
| MlyI | GAGTC | 2 cut(s) | 210, 225 |
| MnlI | CCTC | 4 cut(s) | 16, 249, 434, 551 |
| MseI | TTAA | 2 cut(s) | 483, 504 |
| MspI | CCGG | 1 cut(s) | 298 |
| MspR9I | CCNGG | 1 cut(s) | 30 |
| MvaI | CCWGG | 1 cut(s) | 30 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 53, 166, 360 |
| NdeI | CATATG | 1 cut(s) | 67 |
| NdeII | GATC | 1 cut(s) | 174 |
| NlaIII | CATG | 5 cut(s) | 97, 169, 350, 449, 565 |
| NlaIV | GGNNCC | 1 cut(s) | 27 |
| NmuCI | GTSAC | 3 cut(s) | 76, 340, 389 |
| NspI | RCATGY | 1 cut(s) | 97 |
| NspV | TTCGAA | 1 cut(s) | 193 |
| PaeR7I | CTCGAG | 1 cut(s) | 198 |
| PasI | CCCWGGG | 1 cut(s) | 29 |
| PfeI | GAWTC | 1 cut(s) | 283 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 71, 286 |
| PkrI | GCNGC | 2 cut(s) | 323, 451 |
| PleI | GAGTC | 2 cut(s) | 209, 224 |
| PpsI | GAGTC | 2 cut(s) | 209, 224 |
| PpuMI | RGGWCCY | 1 cut(s) | 25 |
| Psp124BI | GAGCTC | 1 cut(s) | 49 |
| Psp5II | RGGWCCY | 1 cut(s) | 25 |
| Psp6I | CCWGG | 1 cut(s) | 28 |
| PspGI | CCWGG | 1 cut(s) | 28 |
| PspN4I | GGNNCC | 1 cut(s) | 27 |
| PspPI | GGNCC | 3 cut(s) | 25, 368, 508 |
| PspPPI | RGGWCCY | 1 cut(s) | 25 |
| PspXI | VCTCGAGB | 1 cut(s) | 198 |
| PstNI | CAGNNNCTG | 1 cut(s) | 286 |
| RsaI | GTAC | 2 cut(s) | 138, 435 |
| RsaNI | GTAC | 2 cut(s) | 137, 434 |
| SacI | GAGCTC | 1 cut(s) | 49 |
| SaqAI | TTAA | 2 cut(s) | 483, 504 |
| SatI | GCNGC | 2 cut(s) | 322, 450 |
| Sau3AI | GATC | 1 cut(s) | 174 |
| Sau96I | GGNCC | 3 cut(s) | 25, 368, 508 |
| SchI | GAGTC | 2 cut(s) | 210, 225 |
| ScrFI | CCNGG | 1 cut(s) | 30 |
| SduI | GDGCHC | 1 cut(s) | 49 |
| SetI | ASST | 6 cut(s) | 49, 187, 312, 425, 445, 497 |
| SfaNI | GCATC | 2 cut(s) | 171, 557 |
| Sfr274I | CTCGAG | 1 cut(s) | 198 |
| SfuI | TTCGAA | 1 cut(s) | 193 |
| SinI | GGWCC | 1 cut(s) | 25 |
| SlaI | CTCGAG | 1 cut(s) | 198 |
| SmlI | CTYRAG | 1 cut(s) | 198 |
| SmoI | CTYRAG | 1 cut(s) | 198 |
| SpeI | ACTAGT | 1 cut(s) | 337 |
| Sse9I | AATT | 1 cut(s) | 293 |
| SsiI | CCGC | 1 cut(s) | 324 |
| SspMI | CTAG | 3 cut(s) | 59, 83, 338 |
| SstI | GAGCTC | 1 cut(s) | 49 |
| StyD4I | CCNGG | 1 cut(s) | 28 |
| StyI | CCWWGG | 1 cut(s) | 518 |
| TaaI | ACNGT | 2 cut(s) | 141, 433 |
| TaiI | ACGT | 1 cut(s) | 425 |
| TaqI | TCGA | 3 cut(s) | 193, 199, 219 |
| TaqII | GACCGA | 1 cut(s) | 141 |
| TasI | AATT | 1 cut(s) | 293 |
| TfiI | GAWTC | 1 cut(s) | 283 |
| Tru1I | TTAA | 2 cut(s) | 483, 504 |
| Tru9I | TTAA | 2 cut(s) | 483, 504 |
| TscAI | CASTG | 3 cut(s) | 132, 402, 558 |
| TseFI | GTSAC | 3 cut(s) | 76, 340, 389 |
| TseI | GCWGC | 2 cut(s) | 321, 449 |
| Tsp45I | GTSAC | 3 cut(s) | 76, 340, 389 |
| TspDTI | ATGAA | 1 cut(s) | 288 |
| TspRI | CASTG | 3 cut(s) | 132, 402, 558 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 71, 286 |
| VpaK11BI | GGWCC | 1 cut(s) | 25 |
| XceI | RCATGY | 1 cut(s) | 97 |
| XhoI | CTCGAG | 1 cut(s) | 198 |
| XspI | CTAG | 3 cut(s) | 59, 83, 338 |