Rmu_sc0020316.1_g000002

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020316.1
OG0000308
Physical Location & Seq
Forward (+)
12655 .. 13505
851 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020316.1_g000002.1.cds

Sequence Viewer

Length: 768 bp
atggcctctgttgatgcgatggcagccaggcttacctcctcccttgccattgcggaagggaagcgacctgtggacctttgtcggtccaagggacttcgtcagccggaagtgtttatgcttggaaagctcctaactgctagggagtatacgcgccgctcttttctgggcatgttcagggtttggaagggaggcccttggggattcaatcgagccctgattgctttagtggaatataatggtgtgagcccggtgatgtcaatctcgctggaaaagtcatcgtattggatgacgttgatgggggttcctccggccttccgatccgagagaatcatgcgtctcgttagaagcacgcttggtgaattcatggagtatgataaggtggctttcagaaccagggtgctgcggattcgggtcgagattgattatacaaggtctttgctactcaagagtcgtttctgggttgaggatgtcgttcagttcatggtacaattcaagtacgataagatgtttggtcagtgtggaagatgtgggctggttacacatgtcgggttaccatgttcgaggcctgagttactggaggaagaggaggggtctgttggtatggtggagcactaccagaaaaaaggtcttttacggcccacaatgtgcgccgtaaatgacattttacggcccacaaatgcaggccgtaaatgggcatgccgtaaaagacaactatcatttacggcgcacattatgtgcgctgtaaataagtctcaatatgcgtcgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

255

Amino Acids

29.19

Weight (kDa)

9.89

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000308)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24891 FvH4_4g09031
rosa_multiflora Rmu_co8001542.1_g000001 Rmu_co8074240.1_g000001 Rmu_co8092076.1_g000001 Rmu_co8136936.1_g000001 Rmu_co8303129.1_g000001 Rmu_co8326579.1_g000001 Rmu_co8378475.1_g000001 Rmu_co8409011.1_g000001 Rmu_co8449663.1_g000001 Rmu_co8463085.1_g000001 Rmu_sc0000146.1_g000025 Rmu_sc0000294.1_g000018 Rmu_sc0000299.1_g000003 Rmu_sc0000342.1_g000003 Rmu_sc0000359.1_g000009 Rmu_sc0000431.1_g000022 Rmu_sc0000442.1_g000006 Rmu_sc0000458.1_g000011 Rmu_sc0000560.1_g000013 Rmu_sc0000594.1_g000075 Rmu_sc0000740.1_g000033 Rmu_sc0000839.1_g000002 Rmu_sc0000939.1_g000034 Rmu_sc0000955.1_g000002 Rmu_sc0001069.1_g000045 Rmu_sc0001074.1_g000019 Rmu_sc0001418.1_g000003 Rmu_sc0001484.1_g000006 Rmu_sc0001499.1_g000062 Rmu_sc0001631.1_g000002 Rmu_sc0001639.1_g000021 Rmu_sc0002146.1_g000007 Rmu_sc0002146.1_g000010 Rmu_sc0002260.1_g000065 Rmu_sc0002273.1_g000005 Rmu_sc0002310.1_g000016 Rmu_sc0002414.1_g000051 Rmu_sc0002479.1_g000003 Rmu_sc0002586.1_g000034 Rmu_sc0002586.1_g000035 Rmu_sc0002589.1_g000034 Rmu_sc0003187.1_g000020 Rmu_sc0003444.1_g000018 Rmu_sc0003633.1_g000041 Rmu_sc0003973.1_g000010 Rmu_sc0004209.1_g000004 Rmu_sc0004927.1_g000020 Rmu_sc0004950.1_g000007 Rmu_sc0004952.1_g000012 Rmu_sc0004966.1_g000001 Rmu_sc0006168.1_g000026 Rmu_sc0006325.1_g000003 Rmu_sc0006413.1_g000015 Rmu_sc0007192.1_g000002 Rmu_sc0007390.1_g000004 Rmu_sc0007847.1_g000003 Rmu_sc0008044.1_g000001 Rmu_sc0008101.1_g000008 Rmu_sc0008442.1_g000015 Rmu_sc0008714.1_g000004 Rmu_sc0009057.1_g000009 Rmu_sc0009916.1_g000015 Rmu_sc0010180.1_g000003 Rmu_sc0010560.1_g000005 Rmu_sc0010715.1_g000005 Rmu_sc0011022.1_g000002 Rmu_sc0011509.1_g000006 Rmu_sc0011831.1_g000006 Rmu_sc0012010.1_g000002 Rmu_sc0013320.1_g000007 Rmu_sc0013801.1_g000006 Rmu_sc0013801.1_g000007 Rmu_sc0013887.1_g000002 Rmu_sc0014485.1_g000014 Rmu_sc0018736.1_g000001 Rmu_sc0019355.1_g000001 Rmu_sc0020316.1_g000002 Rmu_sc0021214.1_g000001 Rmu_sc0021375.1_g000001 Rmu_sc0023412.1_g000001 Rmu_sc0024234.1_g000001 Rmu_sc0027415.1_g000001 Rmu_sc0029109.1_g000001 Rmu_sc0031708.1_g000001 Rmu_sc0035307.1_g000001 Rmu_sc0036546.1_g000001 Rmu_sc0037386.1_g000005 Rmu_sc0039204.1_g000001 Rmu_sc0039709.1_g000001 Rmu_sc0039867.1_g000001 Rmu_sc0040008.1_g000002 Rmu_sc0040009.1_g000002 Rmu_sc0041433.1_g000001 Rmu_sc0043076.1_g000001 Rmu_ssc0000024.1_g000022 Rmu_ssc0000065.1_g000003 Rmu_ssc0000100.1_g000036 Rmu_ssc0000171.1_g000015 Rmu_ssc0000190.1_g000018 Rmu_ssc0000226.1_g000017 Rmu_ssc0000244.1_g000039 Rmu_ssc0000419.1_g000008 Rmu_ssc0000451.1_g000014
rosa_roxburghii Rroxscaffold_1G00006150 Rroxscaffold_3G00219360 Rroxscaffold_5G00368320 Rroxscaffold_7G00191310
rosa_rugosa Rorug04G0060300 Rorug04G0115300 Rorug04G0128800
rosa_samantha Rh1AG029600 Rh2BG406100 Rh4DG128300 Rh5DG195500 Rh6AG427300 Rh6CG440900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 156
AccI GTMKAC 1 cut(s) 146
AccII CGCG 1 cut(s) 151
AciI CCGC 3 cut(s) 53, 154, 403
AclWI GGATC 1 cut(s) 312
AcsI RAATTY 1 cut(s) 359
AdeI CACNNNGTG 1 cut(s) 645
AfaI GTAC 2 cut(s) 486, 497
AfiI CCNNNNNNNGG 1 cut(s) 691
AflIII ACRYGT 1 cut(s) 541
AgsI TTSAA 2 cut(s) 205, 493
AjnI CCWGG 2 cut(s) 26, 392
AluBI AGCT 1 cut(s) 127
AluI AGCT 1 cut(s) 127
Alw21I GWGCWC 1 cut(s) 612
Alw26I GTCTC 2 cut(s) 341, 756
AlwI GGATC 1 cut(s) 312
AoxI GGCC 7 cut(s) 3, 190, 309, 563, 635, 668, 682
ApeKI GCWGC 2 cut(s) 23, 400
ApoI RAATTY 1 cut(s) 359
AspLEI GCGC 4 cut(s) 153, 650, 727, 740
AspS9I GGNCC 5 cut(s) 73, 84, 191, 636, 669
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 262, 368
AvaII GGWCC 2 cut(s) 73, 84
BanII GRGCYC 2 cut(s) 214, 248
Bbv12I GWGCWC 1 cut(s) 612
BbvI GCAGC 2 cut(s) 35, 387
BccI CCATC 2 cut(s) 13, 289
BceAI ACGGC 6 cut(s) 635, 650, 669, 683, 684, 738
BciT130I CCWGG 2 cut(s) 28, 394
BcnI CCSGG 1 cut(s) 248
BcoDI GTCTC 2 cut(s) 341, 756
BfaI CTAG 1 cut(s) 138
BisI GCNGC 3 cut(s) 24, 154, 401
BlsI GCNGC 3 cut(s) 25, 155, 402
Bme1390I CCNGG 3 cut(s) 28, 248, 394
Bme18I GGWCC 2 cut(s) 73, 84
BmgT120I GGNCC 5 cut(s) 73, 84, 191, 636, 669
BmiI GGNNCC 1 cut(s) 303
BmrFI CCNGG 3 cut(s) 28, 248, 394
BmsI GCATC 1 cut(s) 4
BoxI GACNNNNGTC 1 cut(s) 78
BpmI CTGGAG 1 cut(s) 596
BpuEI CTTGAG 1 cut(s) 428
BpuMI CCSGG 1 cut(s) 248
BsaBI GATNNNNATC 1 cut(s) 257
BsaJI CCNNGG 3 cut(s) 87, 194, 393
Bsc4I CCNNNNNNNGG 1 cut(s) 691
Bse1I ACTGG 1 cut(s) 579
Bse3DI GCAATG 1 cut(s) 48
Bse8I GATNNNNATC 1 cut(s) 257
BseBI CCWGG 2 cut(s) 28, 394
BseDI CCNNGG 3 cut(s) 87, 194, 393
BseGI GGATG 2 cut(s) 291, 472
BseJI GATNNNNATC 1 cut(s) 257
BseLI CCNNNNNNNGG 1 cut(s) 691
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 1 cut(s) 558
BseNI ACTGG 1 cut(s) 579
BseRI GAGGAG 2 cut(s) 28, 599
BseXI GCAGC 2 cut(s) 35, 387
Bsh1236I CGCG 1 cut(s) 151
BshFI GGCC 7 cut(s) 5, 192, 311, 565, 637, 670, 684
BsiHKAI GWGCWC 1 cut(s) 612
BsiSI CCGG 3 cut(s) 104, 248, 308
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 1 cut(s) 691
BsmAI GTCTC 2 cut(s) 341, 756
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 1 cut(s) 105
BsnI GGCC 7 cut(s) 5, 192, 311, 565, 637, 670, 684
Bsp1286I GDGCHC 3 cut(s) 214, 248, 612
Bsp143I GATC 1 cut(s) 317
BspACI CCGC 3 cut(s) 53, 154, 403
BspANI GGCC 7 cut(s) 5, 192, 311, 565, 637, 670, 684
BspCNI CTCAG 1 cut(s) 559
BspFNI CGCG 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 303
BspPI GGATC 1 cut(s) 312
BsrBI CCGCTC 1 cut(s) 156
BsrDI GCAATG 1 cut(s) 48
BsrI ACTGG 1 cut(s) 579
BssECI CCNNGG 3 cut(s) 87, 194, 393
BssMI GATC 1 cut(s) 317
BssNAI GTATAC 1 cut(s) 147
BssT1I CCWWGG 2 cut(s) 87, 194
Bst1107I GTATAC 1 cut(s) 147
Bst2UI CCWGG 2 cut(s) 28, 394
Bst6I CTCTTC 1 cut(s) 576
BstC8I GCNNGC 3 cut(s) 350, 682, 697
BstDEI CTNAG 1 cut(s) 567
BstEII GGTNACC 1 cut(s) 549
BstF5I GGATG 2 cut(s) 291, 472
BstFNI CGCG 1 cut(s) 151
BstHHI GCGC 4 cut(s) 153, 650, 727, 740
BstKTI GATC 1 cut(s) 320
BstMAI GTCTC 2 cut(s) 341, 756
BstMBI GATC 1 cut(s) 317
BstMWI GCNNNNNNNGC 3 cut(s) 23, 124, 218
BstNI CCWGG 2 cut(s) 28, 394
BstNSI RCATGY 3 cut(s) 172, 545, 699
BstPAI GACNNNNGTC 1 cut(s) 78
BstPI GGTNACC 1 cut(s) 549
BstSCI CCNGG 3 cut(s) 26, 246, 392
BstUI CGCG 1 cut(s) 151
BstV1I GCAGC 2 cut(s) 35, 387
BstZ17I GTATAC 1 cut(s) 147
BsuRI GGCC 7 cut(s) 5, 192, 311, 565, 637, 670, 684
BtgZI GCGATG 1 cut(s) 32
BtsCI GGATG 2 cut(s) 291, 472
BtsIMutI CAGTG 1 cut(s) 521
Cac8I GCNNGC 3 cut(s) 350, 682, 697
CfoI GCGC 4 cut(s) 153, 650, 727, 740
Cfr13I GGNCC 5 cut(s) 73, 84, 191, 636, 669
CseI GACGC 2 cut(s) 323, 750
Csp6I GTAC 2 cut(s) 485, 496
CviAII CATG 7 cut(s) 169, 331, 364, 481, 542, 555, 696
CviQI GTAC 2 cut(s) 485, 496
DdeI CTNAG 1 cut(s) 567
DpnI GATC 1 cut(s) 319
DpnII GATC 1 cut(s) 317
DraIII CACNNNGTG 1 cut(s) 645
Eam1104I CTCTTC 1 cut(s) 576
EarI CTCTTC 1 cut(s) 576
Eco130I CCWWGG 2 cut(s) 87, 194
Eco147I AGGCCT 1 cut(s) 565
Eco24I GRGCYC 2 cut(s) 214, 248
Eco47I GGWCC 2 cut(s) 73, 84
Eco91I GGTNACC 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 191
EcoO65I GGTNACC 1 cut(s) 549
EcoRI GAATTC 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 26, 392
EcoT14I CCWWGG 2 cut(s) 87, 194
EcoT38I GRGCYC 2 cut(s) 214, 248
ErhI CCWWGG 2 cut(s) 87, 194
Esp3I CGTCTC 1 cut(s) 341
FaeI CATG 7 cut(s) 172, 334, 367, 484, 545, 558, 699
FaqI GGGAC 1 cut(s) 105
FatI CATG 7 cut(s) 168, 330, 363, 480, 541, 554, 695
FblI GTMKAC 1 cut(s) 146
Fnu4HI GCNGC 3 cut(s) 24, 154, 401
FokI GGATG 2 cut(s) 298, 479
FriOI GRGCYC 2 cut(s) 214, 248
Fsp4HI GCNGC 3 cut(s) 24, 154, 401
FspBI CTAG 1 cut(s) 138
GlaI GCGC 4 cut(s) 152, 649, 726, 739
GluI GCNGC 3 cut(s) 24, 154, 401
GsuI CTGGAG 1 cut(s) 596
HaeIII GGCC 7 cut(s) 5, 192, 311, 565, 637, 670, 684
HapII CCGG 3 cut(s) 104, 248, 308
HgaI GACGC 2 cut(s) 323, 750
HhaI GCGC 4 cut(s) 153, 650, 727, 740
Hin1II CATG 7 cut(s) 172, 334, 367, 484, 545, 558, 699
Hin6I GCGC 4 cut(s) 151, 648, 725, 738
HinP1I GCGC 4 cut(s) 151, 648, 725, 738
HinfI GANTC 4 cut(s) 201, 327, 406, 448
HpaII CCGG 3 cut(s) 104, 248, 308
HphI GGTGA 2 cut(s) 262, 368
Hpy166II GTNNAC 2 cut(s) 73, 147
Hpy188I TCNGA 3 cut(s) 317, 322, 389
Hpy188III TCNNGA 2 cut(s) 415, 445
Hpy8I GTNNAC 2 cut(s) 73, 147
Hpy99I CGWCG 1 cut(s) 766
HpyAV CCTTC 3 cut(s) 50, 178, 322
HpyCH4IV ACGT 1 cut(s) 290
HpyCH4V TGCA 1 cut(s) 680
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 124, 218
HpyF3I CTNAG 1 cut(s) 567
HpySE526I ACGT 1 cut(s) 290
Hsp92II CATG 7 cut(s) 172, 334, 367, 484, 545, 558, 699
HspAI GCGC 4 cut(s) 151, 648, 725, 738
Kzo9I GATC 1 cut(s) 317
LmnI GCTCC 2 cut(s) 132, 607
Lsp1109I GCAGC 2 cut(s) 35, 387
LweI GCATC 1 cut(s) 4
MaeI CTAG 1 cut(s) 138
MaeII ACGT 1 cut(s) 290
MaeIII GTNAC 3 cut(s) 535, 549, 570
MalI GATC 1 cut(s) 319
MbiI CCGCTC 1 cut(s) 156
MboI GATC 1 cut(s) 317
MboII GAAGA 2 cut(s) 534, 593
MhlI GDGCHC 3 cut(s) 214, 248, 612
MluCI AATT 2 cut(s) 359, 488
MlyI GAGTC 1 cut(s) 457
MspI CCGG 3 cut(s) 104, 248, 308
MspR9I CCNGG 3 cut(s) 28, 248, 394
MvaI CCWGG 2 cut(s) 28, 394
MvnI CGCG 1 cut(s) 151
MwoI GCNNNNNNNGC 3 cut(s) 23, 124, 218
NciI CCSGG 1 cut(s) 248
NdeII GATC 1 cut(s) 317
NlaIII CATG 7 cut(s) 172, 334, 367, 484, 545, 558, 699
NlaIV GGNNCC 1 cut(s) 303
NspI RCATGY 3 cut(s) 172, 545, 699
PaeI GCATGC 1 cut(s) 699
PceI AGGCCT 1 cut(s) 565
PciI ACATGT 1 cut(s) 541
PfeI GAWTC 3 cut(s) 201, 327, 406
PflFI GACNNNGTC 1 cut(s) 96
PkrI GCNGC 3 cut(s) 25, 155, 402
PleI GAGTC 1 cut(s) 456
PpsI GAGTC 1 cut(s) 456
PscI ACATGT 1 cut(s) 541
PshAI GACNNNNGTC 1 cut(s) 78
Psp6I CCWGG 2 cut(s) 26, 392
PspEI GGTNACC 1 cut(s) 549
PspGI CCWGG 2 cut(s) 26, 392
PspN4I GGNNCC 1 cut(s) 303
PspPI GGNCC 5 cut(s) 73, 84, 191, 636, 669
PsyI GACNNNGTC 1 cut(s) 96
RsaI GTAC 2 cut(s) 486, 497
RsaNI GTAC 2 cut(s) 485, 496
SatI GCNGC 3 cut(s) 24, 154, 401
Sau3AI GATC 1 cut(s) 317
Sau96I GGNCC 5 cut(s) 73, 84, 191, 636, 669
SchI GAGTC 1 cut(s) 457
ScrFI CCNGG 3 cut(s) 28, 248, 394
SduI GDGCHC 3 cut(s) 214, 248, 612
SetI ASST 8 cut(s) 38, 70, 78, 129, 293, 381, 434, 628
SfaNI GCATC 1 cut(s) 4
SinI GGWCC 2 cut(s) 73, 84
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
SphI GCATGC 1 cut(s) 699
Sse9I AATT 2 cut(s) 359, 488
SseBI AGGCCT 1 cut(s) 565
SsiI CCGC 3 cut(s) 53, 154, 403
SspMI CTAG 1 cut(s) 138
StuI AGGCCT 1 cut(s) 565
StyD4I CCNGG 3 cut(s) 26, 246, 392
StyI CCWWGG 2 cut(s) 87, 194
TaiI ACGT 1 cut(s) 293
TaqI TCGA 3 cut(s) 208, 414, 560
TaqII GACCGA 1 cut(s) 72
TasI AATT 2 cut(s) 359, 488
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 3 cut(s) 201, 327, 406
TscAI CASTG 1 cut(s) 521
TseI GCWGC 2 cut(s) 23, 400
TspDTI ATGAA 2 cut(s) 352, 469
TspRI CASTG 1 cut(s) 521
Tth111I GACNNNGTC 1 cut(s) 96
VpaK11BI GGWCC 2 cut(s) 73, 84
XapI RAATTY 1 cut(s) 359
XceI RCATGY 3 cut(s) 172, 545, 699
XmiI GTMKAC 1 cut(s) 146
XspI CTAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.