Rmu_ssc0000226.1_g000017

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000226.1
OG0000308
Physical Location & Seq
Forward (+)
68839 .. 70889
2051 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000226.1_g000017.1.cds

Sequence Viewer

Length: 819 bp
atgattggcaagctccttacggggagggaatactctcgcagatcgttcctgggcatgtttcgagctgcatggaggattaatggcagcctccgggtggaggaagtcggagaagatgacagagttttgctttccttcacagacccatttgatgttgatcgtgtctcgaaaggcggacaatggggctataacagagctccgatagacctttcatcttatgacggtgtcagctcggcggtggatgtacccctcacgaaatcggagcgcattatgcgcctcattggcagcacgattggcgatctcaaggaaatcgatcgtctggcattcaggaatcgagtcatgcgtattcgggttgagatcgactatgtgaaacctctgttgtcccgtcgtcgattctgggtagaagatgaagtccagtttatagtgcagttcaggtatgacaagattttcggtcgctgcggcacttgtggattggttactcatgttggcctcccctgctccgggccggaactagttgaagacaatgatacaccgatgggggcagtagcaccactcacggccttagctgtcatgcgggaacaacctgtaccgaaccctcattctactagggtgggcagcacctacaacgaaatcaagaccaagatcatcaataccaagatctcaagagagagcggtgtagagaatcttgatgcgaacctctccggcctgtggcggcgccacctggacgtcttcgataaccaaaggcttgttgggttcccaggccaccgccgctttgtaggtgatgacttgaccttgggtcgccatggatttgactgtaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

272

Amino Acids

30.82

Weight (kDa)

8.69

Isoelectric Point (pI)

33.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000308)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24891 FvH4_4g09031
rosa_multiflora Rmu_co8001542.1_g000001 Rmu_co8074240.1_g000001 Rmu_co8092076.1_g000001 Rmu_co8136936.1_g000001 Rmu_co8303129.1_g000001 Rmu_co8326579.1_g000001 Rmu_co8378475.1_g000001 Rmu_co8409011.1_g000001 Rmu_co8449663.1_g000001 Rmu_co8463085.1_g000001 Rmu_sc0000146.1_g000025 Rmu_sc0000294.1_g000018 Rmu_sc0000299.1_g000003 Rmu_sc0000342.1_g000003 Rmu_sc0000359.1_g000009 Rmu_sc0000431.1_g000022 Rmu_sc0000442.1_g000006 Rmu_sc0000458.1_g000011 Rmu_sc0000560.1_g000013 Rmu_sc0000594.1_g000075 Rmu_sc0000740.1_g000033 Rmu_sc0000839.1_g000002 Rmu_sc0000939.1_g000034 Rmu_sc0000955.1_g000002 Rmu_sc0001069.1_g000045 Rmu_sc0001074.1_g000019 Rmu_sc0001418.1_g000003 Rmu_sc0001484.1_g000006 Rmu_sc0001499.1_g000062 Rmu_sc0001631.1_g000002 Rmu_sc0001639.1_g000021 Rmu_sc0002146.1_g000007 Rmu_sc0002146.1_g000010 Rmu_sc0002260.1_g000065 Rmu_sc0002273.1_g000005 Rmu_sc0002310.1_g000016 Rmu_sc0002414.1_g000051 Rmu_sc0002479.1_g000003 Rmu_sc0002586.1_g000034 Rmu_sc0002586.1_g000035 Rmu_sc0002589.1_g000034 Rmu_sc0003187.1_g000020 Rmu_sc0003444.1_g000018 Rmu_sc0003633.1_g000041 Rmu_sc0003973.1_g000010 Rmu_sc0004209.1_g000004 Rmu_sc0004927.1_g000020 Rmu_sc0004950.1_g000007 Rmu_sc0004952.1_g000012 Rmu_sc0004966.1_g000001 Rmu_sc0006168.1_g000026 Rmu_sc0006325.1_g000003 Rmu_sc0006413.1_g000015 Rmu_sc0007192.1_g000002 Rmu_sc0007390.1_g000004 Rmu_sc0007847.1_g000003 Rmu_sc0008044.1_g000001 Rmu_sc0008101.1_g000008 Rmu_sc0008442.1_g000015 Rmu_sc0008714.1_g000004 Rmu_sc0009057.1_g000009 Rmu_sc0009916.1_g000015 Rmu_sc0010180.1_g000003 Rmu_sc0010560.1_g000005 Rmu_sc0010715.1_g000005 Rmu_sc0011022.1_g000002 Rmu_sc0011509.1_g000006 Rmu_sc0011831.1_g000006 Rmu_sc0012010.1_g000002 Rmu_sc0013320.1_g000007 Rmu_sc0013801.1_g000006 Rmu_sc0013801.1_g000007 Rmu_sc0013887.1_g000002 Rmu_sc0014485.1_g000014 Rmu_sc0018736.1_g000001 Rmu_sc0019355.1_g000001 Rmu_sc0020316.1_g000002 Rmu_sc0021214.1_g000001 Rmu_sc0021375.1_g000001 Rmu_sc0023412.1_g000001 Rmu_sc0024234.1_g000001 Rmu_sc0027415.1_g000001 Rmu_sc0029109.1_g000001 Rmu_sc0031708.1_g000001 Rmu_sc0035307.1_g000001 Rmu_sc0036546.1_g000001 Rmu_sc0037386.1_g000005 Rmu_sc0039204.1_g000001 Rmu_sc0039709.1_g000001 Rmu_sc0039867.1_g000001 Rmu_sc0040008.1_g000002 Rmu_sc0040009.1_g000002 Rmu_sc0041433.1_g000001 Rmu_sc0043076.1_g000001 Rmu_ssc0000024.1_g000022 Rmu_ssc0000065.1_g000003 Rmu_ssc0000100.1_g000036 Rmu_ssc0000171.1_g000015 Rmu_ssc0000190.1_g000018 Rmu_ssc0000226.1_g000017 Rmu_ssc0000244.1_g000039 Rmu_ssc0000419.1_g000008 Rmu_ssc0000451.1_g000014
rosa_roxburghii Rroxscaffold_1G00006150 Rroxscaffold_3G00219360 Rroxscaffold_5G00368320 Rroxscaffold_7G00191310
rosa_rugosa Rorug04G0060300 Rorug04G0115300 Rorug04G0128800
rosa_samantha Rh1AG029600 Rh2BG406100 Rh4DG128300 Rh5DG195500 Rh6AG427300 Rh6CG440900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 726
AccB1I GGYRCC 1 cut(s) 711
AccBSI CCGCTC 1 cut(s) 669
AciI CCGC 8 cut(s) 171, 233, 456, 571, 669, 709, 763, 766
AcyI GRCGYC 2 cut(s) 712, 723
AfaI GTAC 2 cut(s) 243, 585
AfiI CCNNNNNNNGG 5 cut(s) 94, 97, 497, 498, 705
AgsI TTSAA 1 cut(s) 515
AhdI GACNNNNNGTC 1 cut(s) 792
AhlI ACTAGT 1 cut(s) 508
AjnI CCWGG 3 cut(s) 48, 717, 754
AluBI AGCT 5 cut(s) 13, 65, 194, 228, 563
AluI AGCT 5 cut(s) 13, 65, 194, 228, 563
Alw21I GWGCWC 1 cut(s) 196
Alw26I GTCTC 1 cut(s) 166
AoxI GGCC 5 cut(s) 484, 500, 555, 700, 757
ApeKI GCWGC 5 cut(s) 65, 84, 282, 453, 612
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AseI ATTAAT 1 cut(s) 78
AspLEI GCGC 3 cut(s) 264, 273, 714
AspS9I GGNCC 1 cut(s) 500
AsuC2I CCSGG 2 cut(s) 92, 499
AsuHPI GGTGA 1 cut(s) 788
BanI GGYRCC 1 cut(s) 711
BanII GRGCYC 1 cut(s) 196
BbsI GAAGAC 2 cut(s) 522, 718
Bbv12I GWGCWC 1 cut(s) 196
BbvI GCAGC 5 cut(s) 52, 96, 294, 440, 624
BccI CCATC 1 cut(s) 526
BceAI ACGGC 1 cut(s) 570
BciT130I CCWGG 3 cut(s) 50, 719, 756
BcnI CCSGG 2 cut(s) 92, 499
BcoDI GTCTC 1 cut(s) 166
BcuI ACTAGT 1 cut(s) 508
BfaI CTAG 2 cut(s) 509, 603
BfoI RGCGCY 1 cut(s) 715
BglI GCCNNNNNGGC 1 cut(s) 279
BglII AGATCT 1 cut(s) 654
BisI GCNGC 8 cut(s) 66, 85, 283, 454, 457, 613, 710, 766
BlsI GCNGC 8 cut(s) 67, 86, 284, 455, 458, 614, 711, 767
Bme1390I CCNGG 5 cut(s) 50, 92, 499, 719, 756
BmeRI GACNNNNNGTC 1 cut(s) 792
BmgT120I GGNCC 1 cut(s) 500
BmiI GGNNCC 2 cut(s) 713, 752
BmrFI CCNGG 5 cut(s) 50, 92, 499, 719, 756
BmsI GCATC 1 cut(s) 676
BpiI GAAGAC 2 cut(s) 522, 718
Bpu10I CCTNAGC 1 cut(s) 559
BpuEI CTTGAG 2 cut(s) 284, 643
BpuMI CCSGG 2 cut(s) 92, 499
Bsa29I ATCGAT 1 cut(s) 309
BsaBI GATNNNNATC 1 cut(s) 153
BsaHI GRCGYC 2 cut(s) 712, 723
BsaJI CCNNGG 4 cut(s) 49, 754, 789, 799
Bsc4I CCNNNNNNNGG 5 cut(s) 94, 97, 497, 498, 705
Bse1I ACTGG 1 cut(s) 412
Bse8I GATNNNNATC 1 cut(s) 153
BseBI CCWGG 3 cut(s) 50, 719, 756
BseCI ATCGAT 1 cut(s) 309
BseDI CCNNGG 4 cut(s) 49, 754, 789, 799
BseGI GGATG 1 cut(s) 244
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 5 cut(s) 94, 97, 497, 498, 705
BseNI ACTGG 1 cut(s) 412
BseXI GCAGC 5 cut(s) 52, 96, 294, 440, 624
BsgI GTGCAG 1 cut(s) 443
Bsh1285I CGRYCG 2 cut(s) 313, 451
BshFI GGCC 5 cut(s) 486, 502, 557, 702, 759
BshNI GGYRCC 1 cut(s) 711
BshVI ATCGAT 1 cut(s) 309
BsiEI CGRYCG 2 cut(s) 313, 451
BsiHKAI GWGCWC 1 cut(s) 196
BsiSI CCGG 4 cut(s) 91, 498, 503, 699
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 5 cut(s) 94, 97, 497, 498, 705
BsmAI GTCTC 1 cut(s) 166
BsmFI GGGAC 1 cut(s) 364
BsmI GAATGC 1 cut(s) 320
BsnI GGCC 5 cut(s) 486, 502, 557, 702, 759
Bsp1286I GDGCHC 1 cut(s) 196
Bsp143I GATC 7 cut(s) 41, 154, 295, 310, 354, 639, 654
Bsp19I CCATGG 1 cut(s) 799
BspACI CCGC 8 cut(s) 171, 233, 456, 571, 669, 709, 763, 766
BspANI GGCC 5 cut(s) 486, 502, 557, 702, 759
BspDI ATCGAT 1 cut(s) 309
BspLI GGNNCC 2 cut(s) 713, 752
BspT107I GGYRCC 1 cut(s) 711
BsrBI CCGCTC 1 cut(s) 669
BsrI ACTGG 1 cut(s) 412
BssECI CCNNGG 4 cut(s) 49, 754, 789, 799
BssMI GATC 7 cut(s) 41, 154, 295, 310, 354, 639, 654
BssNI GRCGYC 2 cut(s) 712, 723
BssT1I CCWWGG 2 cut(s) 789, 799
Bst2UI CCWGG 3 cut(s) 50, 719, 756
Bst4CI ACNGT 2 cut(s) 221, 812
BstACI GRCGYC 2 cut(s) 712, 723
BstC8I GCNNGC 1 cut(s) 11
BstDEI CTNAG 1 cut(s) 559
BstDSI CCRYGG 1 cut(s) 799
BstF5I GGATG 1 cut(s) 244
BstH2I RGCGCY 1 cut(s) 715
BstHHI GCGC 3 cut(s) 264, 273, 714
BstKTI GATC 7 cut(s) 44, 157, 298, 313, 357, 642, 657
BstMAI GTCTC 1 cut(s) 166
BstMBI GATC 7 cut(s) 41, 154, 295, 310, 354, 639, 654
BstMCI CGRYCG 2 cut(s) 313, 451
BstMWI GCNNNNNNNGC 6 cut(s) 268, 270, 279, 291, 492, 765
BstNI CCWGG 3 cut(s) 50, 719, 756
BstNSI RCATGY 1 cut(s) 58
BstSCI CCNGG 5 cut(s) 48, 90, 497, 717, 754
BstV1I GCAGC 5 cut(s) 52, 96, 294, 440, 624
BstV2I GAAGAC 2 cut(s) 522, 718
BstX2I RGATCY 1 cut(s) 654
BstYI RGATCY 1 cut(s) 654
Bsu15I ATCGAT 1 cut(s) 309
BsuRI GGCC 5 cut(s) 486, 502, 557, 702, 759
BsuTUI ATCGAT 1 cut(s) 309
BtgI CCRYGG 1 cut(s) 799
BtsCI GGATG 1 cut(s) 244
Cac8I GCNNGC 1 cut(s) 11
CfoI GCGC 3 cut(s) 264, 273, 714
Cfr13I GGNCC 1 cut(s) 500
ClaI ATCGAT 1 cut(s) 309
Csp6I GTAC 2 cut(s) 242, 584
CviAII CATG 6 cut(s) 55, 69, 337, 479, 568, 800
CviQI GTAC 2 cut(s) 242, 584
DdeI CTNAG 1 cut(s) 559
DinI GGCGCC 1 cut(s) 713
DpnI GATC 7 cut(s) 43, 156, 297, 312, 356, 641, 656
DpnII GATC 7 cut(s) 41, 154, 295, 310, 354, 639, 654
DriI GACNNNNNGTC 1 cut(s) 792
Eam1105I GACNNNNNGTC 1 cut(s) 792
EciI GGCGGA 1 cut(s) 186
Ecl136II GAGCTC 1 cut(s) 194
Eco130I CCWWGG 2 cut(s) 789, 799
Eco24I GRGCYC 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 194
EcoICRI GAGCTC 1 cut(s) 194
EcoRII CCWGG 3 cut(s) 48, 717, 754
EcoT14I CCWWGG 2 cut(s) 789, 799
EcoT38I GRGCYC 1 cut(s) 196
EgeI GGCGCC 1 cut(s) 713
EheI GGCGCC 1 cut(s) 713
ErhI CCWWGG 2 cut(s) 789, 799
FaeI CATG 6 cut(s) 58, 72, 340, 482, 571, 803
FaqI GGGAC 1 cut(s) 364
FatI CATG 6 cut(s) 54, 68, 336, 478, 567, 799
FauI CCCGC 1 cut(s) 564
Fnu4HI GCNGC 8 cut(s) 66, 85, 283, 454, 457, 613, 710, 766
FokI GGATG 1 cut(s) 251
FriOI GRGCYC 1 cut(s) 196
Fsp4HI GCNGC 8 cut(s) 66, 85, 283, 454, 457, 613, 710, 766
FspBI CTAG 2 cut(s) 509, 603
GlaI GCGC 3 cut(s) 263, 272, 713
GluI GCNGC 8 cut(s) 66, 85, 283, 454, 457, 613, 710, 766
HaeII RGCGCY 1 cut(s) 715
HaeIII GGCC 5 cut(s) 486, 502, 557, 702, 759
HapII CCGG 4 cut(s) 91, 498, 503, 699
HhaI GCGC 3 cut(s) 264, 273, 714
Hin1I GRCGYC 2 cut(s) 712, 723
Hin1II CATG 6 cut(s) 58, 72, 340, 482, 571, 803
Hin6I GCGC 3 cut(s) 262, 271, 712
HinP1I GCGC 3 cut(s) 262, 271, 712
HinfI GANTC 4 cut(s) 328, 333, 390, 679
HpaII CCGG 4 cut(s) 91, 498, 503, 699
HphI GGTGA 1 cut(s) 788
Hpy188I TCNGA 3 cut(s) 107, 198, 259
Hpy188III TCNNGA 6 cut(s) 163, 250, 325, 631, 660, 683
Hpy99I CGWCG 2 cut(s) 387, 390
HpyAV CCTTC 1 cut(s) 142
HpyCH4III ACNGT 2 cut(s) 221, 812
HpyCH4IV ACGT 1 cut(s) 723
HpyCH4V TGCA 2 cut(s) 68, 424
HpyF10VI GCNNNNNNNGC 6 cut(s) 268, 270, 279, 291, 492, 765
HpyF3I CTNAG 1 cut(s) 559
HpySE526I ACGT 1 cut(s) 723
Hsp92I GRCGYC 2 cut(s) 712, 723
Hsp92II CATG 6 cut(s) 58, 72, 340, 482, 571, 803
HspAI GCGC 3 cut(s) 262, 271, 712
KasI GGCGCC 1 cut(s) 711
Kzo9I GATC 7 cut(s) 41, 154, 295, 310, 354, 639, 654
LmnI GCTCC 4 cut(s) 18, 199, 259, 500
Lsp1109I GCAGC 5 cut(s) 52, 96, 294, 440, 624
LweI GCATC 1 cut(s) 676
MaeI CTAG 2 cut(s) 509, 603
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 2 cut(s) 472, 812
MalI GATC 7 cut(s) 43, 156, 297, 312, 356, 641, 656
MbiI CCGCTC 1 cut(s) 669
MboI GATC 7 cut(s) 41, 154, 295, 310, 354, 639, 654
MboII GAAGA 4 cut(s) 122, 413, 527, 718
MflI RGATCY 1 cut(s) 654
MhlI GDGCHC 1 cut(s) 196
Mly113I GGCGCC 1 cut(s) 712
MlyI GAGTC 1 cut(s) 342
MmeI TCCRAC 1 cut(s) 85
MseI TTAA 1 cut(s) 78
MspI CCGG 4 cut(s) 91, 498, 503, 699
MspR9I CCNGG 5 cut(s) 50, 92, 499, 719, 756
Mva1269I GAATGC 1 cut(s) 320
MvaI CCWGG 3 cut(s) 50, 719, 756
MwoI GCNNNNNNNGC 6 cut(s) 268, 270, 279, 291, 492, 765
NarI GGCGCC 1 cut(s) 712
NciI CCSGG 2 cut(s) 92, 499
NcoI CCATGG 1 cut(s) 799
NdeII GATC 7 cut(s) 41, 154, 295, 310, 354, 639, 654
NlaIII CATG 6 cut(s) 58, 72, 340, 482, 571, 803
NlaIV GGNNCC 2 cut(s) 713, 752
NmeAIII GCCGAG 1 cut(s) 209
NspI RCATGY 1 cut(s) 58
PcsI WCGNNNNNNNCGW 1 cut(s) 337
PctI GAATGC 1 cut(s) 320
PfeI GAWTC 3 cut(s) 328, 390, 679
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 8 cut(s) 67, 86, 284, 455, 458, 614, 711, 767
Ple19I CGATCG 1 cut(s) 313
PleI GAGTC 1 cut(s) 341
PluTI GGCGCC 1 cut(s) 715
PpsI GAGTC 1 cut(s) 341
PshBI ATTAAT 1 cut(s) 78
Psp124BI GAGCTC 1 cut(s) 196
Psp6I CCWGG 3 cut(s) 48, 717, 754
PspGI CCWGG 3 cut(s) 48, 717, 754
PspN4I GGNNCC 2 cut(s) 713, 752
PspPI GGNCC 1 cut(s) 500
PsrI GAACNNNNNNTAC 2 cut(s) 567, 599
PsuI RGATCY 1 cut(s) 654
PsyI GACNNNGTC 1 cut(s) 221
PvuI CGATCG 1 cut(s) 313
RsaI GTAC 2 cut(s) 243, 585
RsaNI GTAC 2 cut(s) 242, 584
SacI GAGCTC 1 cut(s) 196
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 8 cut(s) 66, 85, 283, 454, 457, 613, 710, 766
Sau3AI GATC 7 cut(s) 41, 154, 295, 310, 354, 639, 654
Sau96I GGNCC 1 cut(s) 500
SchI GAGTC 1 cut(s) 342
ScrFI CCNGG 5 cut(s) 50, 92, 499, 719, 756
SduI GDGCHC 1 cut(s) 196
SfaNI GCATC 1 cut(s) 676
SfoI GGCGCC 1 cut(s) 713
SmlI CTYRAG 2 cut(s) 299, 658
SmoI CTYRAG 2 cut(s) 299, 658
SpeI ACTAGT 1 cut(s) 508
SsiI CCGC 8 cut(s) 171, 233, 456, 571, 669, 709, 763, 766
SspDI GGCGCC 1 cut(s) 711
SspMI CTAG 2 cut(s) 509, 603
SstI GAGCTC 1 cut(s) 196
StyD4I CCNGG 5 cut(s) 48, 90, 497, 717, 754
StyI CCWWGG 2 cut(s) 789, 799
TaaI ACNGT 2 cut(s) 221, 812
TaiI ACGT 1 cut(s) 726
TaqI TCGA 7 cut(s) 61, 164, 309, 331, 357, 388, 729
TaqII GACCGA 1 cut(s) 437
TauI GCSGC 3 cut(s) 459, 712, 768
TfiI GAWTC 3 cut(s) 328, 390, 679
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseI GCWGC 5 cut(s) 65, 84, 282, 453, 612
TspDTI ATGAA 2 cut(s) 198, 420
Tth111I GACNNNGTC 1 cut(s) 221
VspI ATTAAT 1 cut(s) 78
XceI RCATGY 1 cut(s) 58
XcmI CCANNNNNNNNNTGG 1 cut(s) 743
XspI CTAG 2 cut(s) 509, 603
ZraI GACGTC 1 cut(s) 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.