Rorug04G0115300

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000308
Physical Location & Seq
Reverse (-)
18156898 .. 18162671
5774 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0115300.1

Sequence Viewer

Length: 801 bp
ATGAACGTGAGCCAGTTCGACGGAAGCGCCGCCTTCCACGGCGGTGGCTTCATGCCCCCCGCCGCCAACCCAACCCCCGAACACTCTTTCTTTCCTTCCAAGAATCGCGACGTCCAAGCACTGCTTCCAGTGACCCTTAAGCAGATCAGCGACGCCATTGCCGGCGAAAATGGTAAAAGCGATTTCTCTATTGACGGCGTCGATGTGTCCACTGTTAAGCTTGTGGGAGTAGTGCAGGAGTTTGTTGGAAGATGTACAGATGCTACTTTCACTCTTGATGACGGGACCGGCCGGATTGAATGCAACCGATGGTGTCAGGAGCAAATAGACACTGCCGAAATCGAAGAAATCTCGAACGGAATGTACGTTCGGGTTCATGGGCGCTTGAAAACCTTTCAAGGGAAGAAGACCATACAAGTGTTCTCTGTTAGGCCTGTGGATGACTATAATGAGATTGCAAGCCACTTTATCGAGTGTATGTACGTACATTTCTTCAACAGCAAGTTACCGAAACCACAGGTTGGTGTAGGTGTCACAACTCAGCCATATATGACAATTCCACCAAATCAGGTATCTGGACAACATAGCCTCTATGGACAGAGGAGCATTGAAGAAAGGGTCTTACAAGTTTTGAACCAGCCTTTATACCTTTCAAGGGATGCGGGAGCAAAAGCTGAGGATATTGCCCAACAACTGAAAGTACCAGTGAAAGATGTCATACATGCTGCTAGTAATCTTGCTCATGTAGGCAGTATTTACGAGATCGATACGTTGCACTATAAATCTCTTGCCAATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

266

Amino Acids

29.26

Weight (kDa)

5.69

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_anti-codon PF01336 72 - 145 1.1e-09 OB-fold nucleic acid binding domain
RPA_C PF08784 192 - 254 4.7e-07 Replication protein A C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000308)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24891 FvH4_4g09031
rosa_multiflora Rmu_co8001542.1_g000001 Rmu_co8074240.1_g000001 Rmu_co8092076.1_g000001 Rmu_co8136936.1_g000001 Rmu_co8303129.1_g000001 Rmu_co8326579.1_g000001 Rmu_co8378475.1_g000001 Rmu_co8409011.1_g000001 Rmu_co8449663.1_g000001 Rmu_co8463085.1_g000001 Rmu_sc0000146.1_g000025 Rmu_sc0000294.1_g000018 Rmu_sc0000299.1_g000003 Rmu_sc0000342.1_g000003 Rmu_sc0000359.1_g000009 Rmu_sc0000431.1_g000022 Rmu_sc0000442.1_g000006 Rmu_sc0000458.1_g000011 Rmu_sc0000560.1_g000013 Rmu_sc0000594.1_g000075 Rmu_sc0000740.1_g000033 Rmu_sc0000839.1_g000002 Rmu_sc0000939.1_g000034 Rmu_sc0000955.1_g000002 Rmu_sc0001069.1_g000045 Rmu_sc0001074.1_g000019 Rmu_sc0001418.1_g000003 Rmu_sc0001484.1_g000006 Rmu_sc0001499.1_g000062 Rmu_sc0001631.1_g000002 Rmu_sc0001639.1_g000021 Rmu_sc0002146.1_g000007 Rmu_sc0002146.1_g000010 Rmu_sc0002260.1_g000065 Rmu_sc0002273.1_g000005 Rmu_sc0002310.1_g000016 Rmu_sc0002414.1_g000051 Rmu_sc0002479.1_g000003 Rmu_sc0002586.1_g000034 Rmu_sc0002586.1_g000035 Rmu_sc0002589.1_g000034 Rmu_sc0003187.1_g000020 Rmu_sc0003444.1_g000018 Rmu_sc0003633.1_g000041 Rmu_sc0003973.1_g000010 Rmu_sc0004209.1_g000004 Rmu_sc0004927.1_g000020 Rmu_sc0004950.1_g000007 Rmu_sc0004952.1_g000012 Rmu_sc0004966.1_g000001 Rmu_sc0006168.1_g000026 Rmu_sc0006325.1_g000003 Rmu_sc0006413.1_g000015 Rmu_sc0007192.1_g000002 Rmu_sc0007390.1_g000004 Rmu_sc0007847.1_g000003 Rmu_sc0008044.1_g000001 Rmu_sc0008101.1_g000008 Rmu_sc0008442.1_g000015 Rmu_sc0008714.1_g000004 Rmu_sc0009057.1_g000009 Rmu_sc0009916.1_g000015 Rmu_sc0010180.1_g000003 Rmu_sc0010560.1_g000005 Rmu_sc0010715.1_g000005 Rmu_sc0011022.1_g000002 Rmu_sc0011509.1_g000006 Rmu_sc0011831.1_g000006 Rmu_sc0012010.1_g000002 Rmu_sc0013320.1_g000007 Rmu_sc0013801.1_g000006 Rmu_sc0013801.1_g000007 Rmu_sc0013887.1_g000002 Rmu_sc0014485.1_g000014 Rmu_sc0018736.1_g000001 Rmu_sc0019355.1_g000001 Rmu_sc0020316.1_g000002 Rmu_sc0021214.1_g000001 Rmu_sc0021375.1_g000001 Rmu_sc0023412.1_g000001 Rmu_sc0024234.1_g000001 Rmu_sc0027415.1_g000001 Rmu_sc0029109.1_g000001 Rmu_sc0031708.1_g000001 Rmu_sc0035307.1_g000001 Rmu_sc0036546.1_g000001 Rmu_sc0037386.1_g000005 Rmu_sc0039204.1_g000001 Rmu_sc0039709.1_g000001 Rmu_sc0039867.1_g000001 Rmu_sc0040008.1_g000002 Rmu_sc0040009.1_g000002 Rmu_sc0041433.1_g000001 Rmu_sc0043076.1_g000001 Rmu_ssc0000024.1_g000022 Rmu_ssc0000065.1_g000003 Rmu_ssc0000100.1_g000036 Rmu_ssc0000171.1_g000015 Rmu_ssc0000190.1_g000018 Rmu_ssc0000226.1_g000017 Rmu_ssc0000244.1_g000039 Rmu_ssc0000419.1_g000008 Rmu_ssc0000451.1_g000014
rosa_roxburghii Rroxscaffold_1G00006150 Rroxscaffold_3G00219360 Rroxscaffold_5G00368320 Rroxscaffold_7G00191310
rosa_rugosa Rorug04G0060300 Rorug04G0115300 Rorug04G0128800
rosa_samantha Rh1AG029600 Rh2BG406100 Rh4DG128300 Rh5DG195500 Rh6AG427300 Rh6CG440900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 114
AccB7I CCANNNNNTGG 1 cut(s) 521
AccII CGCG 1 cut(s) 108
AciI CCGC 5 cut(s) 30, 42, 60, 63, 662
AcoI YGGCCR 1 cut(s) 289
AcyI GRCGYC 3 cut(s) 111, 153, 198
AfaI GTAC 5 cut(s) 256, 365, 482, 486, 702
AfiI CCNNNNNNNGG 3 cut(s) 399, 521, 655
AflII CTTAAG 1 cut(s) 137
AgsI TTSAA 7 cut(s) 299, 388, 398, 496, 611, 634, 654
AjuI GAANNNNNNNTTGG 2 cut(s) 108, 140
AleI CACNNNNGTG 1 cut(s) 42
AloI GAACNNNNNNTCC 2 cut(s) 351, 383
AluBI AGCT 2 cut(s) 220, 674
AluI AGCT 2 cut(s) 220, 674
AoxI GGCC 2 cut(s) 289, 431
ApeKI GCWGC 1 cut(s) 725
AspLEI GCGC 2 cut(s) 29, 384
AspS9I GGNCC 1 cut(s) 285
AvaII GGWCC 1 cut(s) 285
BbsI GAAGAC 1 cut(s) 413
BbvCI CCTCAGC 1 cut(s) 675
BbvI GCAGC 1 cut(s) 712
BccI CCATC 1 cut(s) 303
BceAI ACGGC 2 cut(s) 55, 211
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BfaI CTAG 1 cut(s) 729
BfoI RGCGCY 2 cut(s) 30, 385
BfrI CTTAAG 1 cut(s) 137
BisI GCNGC 3 cut(s) 30, 63, 726
BlsI GCNGC 3 cut(s) 31, 64, 727
Bme18I GGWCC 1 cut(s) 285
BmgT120I GGNCC 1 cut(s) 285
BmiI GGNNCC 1 cut(s) 286
BmsI GCATC 2 cut(s) 250, 649
BpiI GAAGAC 1 cut(s) 413
Bpu10I CCTNAGC 1 cut(s) 675
Bsa29I ATCGAT 1 cut(s) 765
BsaAI YACGTR 1 cut(s) 484
BsaHI GRCGYC 3 cut(s) 111, 153, 198
BsaJI CCNNGG 1 cut(s) 37
Bsc4I CCNNNNNNNGG 3 cut(s) 399, 521, 655
Bse118I RCCGGY 2 cut(s) 161, 287
Bse1I ACTGG 3 cut(s) 13, 128, 704
Bse3DI GCAATG 1 cut(s) 156
BseCI ATCGAT 1 cut(s) 765
BseDI CCNNGG 1 cut(s) 37
BseGI GGATG 2 cut(s) 445, 664
BseLI CCNNNNNNNGG 3 cut(s) 399, 521, 655
BseMI GCAATG 1 cut(s) 156
BseMII CTCAG 2 cut(s) 554, 666
BseNI ACTGG 3 cut(s) 13, 128, 704
BseRI GAGGAG 1 cut(s) 616
BseX3I CGGCCG 1 cut(s) 289
BseXI GCAGC 1 cut(s) 712
BsgI GTGCAG 1 cut(s) 254
Bsh1236I CGCG 1 cut(s) 108
Bsh1285I CGRYCG 1 cut(s) 292
BshFI GGCC 2 cut(s) 291, 433
BshVI ATCGAT 1 cut(s) 765
BsiEI CGRYCG 1 cut(s) 292
BsiSI CCGG 3 cut(s) 162, 288, 292
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 3 cut(s) 399, 521, 655
BsmFI GGGAC 1 cut(s) 298
BsmI GAATGC 1 cut(s) 305
BsnI GGCC 2 cut(s) 291, 433
Bsp1407I TGTACA 1 cut(s) 254
Bsp143I GATC 2 cut(s) 144, 762
Bsp68I TCGCGA 1 cut(s) 108
BspACI CCGC 5 cut(s) 30, 42, 60, 63, 662
BspANI GGCC 2 cut(s) 291, 433
BspCNI CTCAG 2 cut(s) 553, 667
BspDI ATCGAT 1 cut(s) 765
BspFNI CGCG 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 286
BspTI CTTAAG 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 156
BsrFI RCCGGY 2 cut(s) 161, 287
BsrGI TGTACA 1 cut(s) 254
BsrI ACTGG 3 cut(s) 13, 128, 704
BssAI RCCGGY 2 cut(s) 161, 287
BssECI CCNNGG 1 cut(s) 37
BssMI GATC 2 cut(s) 144, 762
BssNI GRCGYC 3 cut(s) 111, 153, 198
Bst4CI ACNGT 1 cut(s) 214
BstACI GRCGYC 3 cut(s) 111, 153, 198
BstAFI CTTAAG 1 cut(s) 137
BstAUI TGTACA 1 cut(s) 254
BstBAI YACGTR 1 cut(s) 484
BstC8I GCNNGC 2 cut(s) 163, 460
BstDEI CTNAG 2 cut(s) 540, 675
BstDSI CCRYGG 1 cut(s) 37
BstF5I GGATG 2 cut(s) 445, 664
BstFNI CGCG 1 cut(s) 108
BstH2I RGCGCY 2 cut(s) 30, 385
BstHHI GCGC 2 cut(s) 29, 384
BstKTI GATC 2 cut(s) 147, 765
BstMBI GATC 2 cut(s) 144, 762
BstMCI CGRYCG 1 cut(s) 292
BstNSI RCATGY 1 cut(s) 725
BstSNI TACGTA 1 cut(s) 484
BstUI CGCG 1 cut(s) 108
BstV1I GCAGC 1 cut(s) 712
BstV2I GAAGAC 1 cut(s) 413
BstXI CCANNNNNNTGG 1 cut(s) 44
BstZI CGGCCG 1 cut(s) 289
Bsu15I ATCGAT 1 cut(s) 765
BsuRI GGCC 2 cut(s) 291, 433
BsuTUI ATCGAT 1 cut(s) 765
BtgI CCRYGG 1 cut(s) 37
BtsCI GGATG 2 cut(s) 445, 664
BtsI GCAGTG 2 cut(s) 119, 330
BtsIMutI CAGTG 5 cut(s) 119, 135, 210, 330, 711
BtuMI TCGCGA 1 cut(s) 108
Cac8I GCNNGC 2 cut(s) 163, 460
CfoI GCGC 2 cut(s) 29, 384
Cfr10I RCCGGY 2 cut(s) 161, 287
Cfr13I GGNCC 1 cut(s) 285
ClaI ATCGAT 1 cut(s) 765
CseI GACGC 2 cut(s) 161, 187
Csp6I GTAC 5 cut(s) 255, 364, 481, 485, 701
CviAII CATG 4 cut(s) 52, 377, 722, 743
CviQI GTAC 5 cut(s) 255, 364, 481, 485, 701
DdeI CTNAG 2 cut(s) 540, 675
DpnI GATC 2 cut(s) 146, 764
DpnII GATC 2 cut(s) 144, 762
EaeI YGGCCR 1 cut(s) 289
EagI CGGCCG 1 cut(s) 289
EclXI CGGCCG 1 cut(s) 289
Eco105I TACGTA 1 cut(s) 484
Eco147I AGGCCT 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 285
Eco52I CGGCCG 1 cut(s) 289
FaeI CATG 4 cut(s) 55, 380, 725, 746
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FaqI GGGAC 1 cut(s) 298
FatI CATG 4 cut(s) 51, 376, 721, 742
FauI CCCGC 2 cut(s) 67, 655
Fnu4HI GCNGC 3 cut(s) 30, 63, 726
FokI GGATG 2 cut(s) 452, 671
Fsp4HI GCNGC 3 cut(s) 30, 63, 726
FspBI CTAG 1 cut(s) 729
GlaI GCGC 2 cut(s) 28, 383
GluI GCNGC 3 cut(s) 30, 63, 726
HaeII RGCGCY 2 cut(s) 30, 385
HaeIII GGCC 2 cut(s) 291, 433
HapII CCGG 3 cut(s) 162, 288, 292
HgaI GACGC 2 cut(s) 161, 187
HhaI GCGC 2 cut(s) 29, 384
Hin1I GRCGYC 3 cut(s) 111, 153, 198
Hin1II CATG 4 cut(s) 55, 380, 725, 746
Hin6I GCGC 2 cut(s) 27, 382
HinP1I GCGC 2 cut(s) 27, 382
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 1 cut(s) 103
HpaII CCGG 3 cut(s) 162, 288, 292
Hpy166II GTNNAC 1 cut(s) 210
Hpy188III TCNNGA 5 cut(s) 107, 275, 317, 352, 576
Hpy8I GTNNAC 1 cut(s) 210
Hpy99I CGWCG 4 cut(s) 23, 113, 155, 203
HpyAV CCTTC 2 cut(s) 43, 105
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4IV ACGT 5 cut(s) 6, 111, 366, 483, 770
HpyCH4V TGCA 4 cut(s) 235, 303, 458, 775
HpyF3I CTNAG 2 cut(s) 540, 675
HpySE526I ACGT 5 cut(s) 6, 111, 366, 483, 770
Hsp92I GRCGYC 3 cut(s) 111, 153, 198
Hsp92II CATG 4 cut(s) 55, 380, 725, 746
HspAI GCGC 2 cut(s) 27, 382
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Kzo9I GATC 2 cut(s) 144, 762
LmnI GCTCC 3 cut(s) 319, 603, 665
Lsp1109I GCAGC 1 cut(s) 712
LweI GCATC 2 cut(s) 250, 649
MaeI CTAG 1 cut(s) 729
MaeII ACGT 5 cut(s) 6, 111, 366, 483, 770
MaeIII GTNAC 3 cut(s) 130, 504, 532
MalI GATC 2 cut(s) 146, 764
MboI GATC 2 cut(s) 144, 762
MboII GAAGA 6 cut(s) 261, 356, 415, 418, 484, 623
MluCI AATT 1 cut(s) 555
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 3 cut(s) 594, 599, 670
MroNI GCCGGC 1 cut(s) 161
MseI TTAA 2 cut(s) 138, 216
MslI CAYNNNNRTG 2 cut(s) 42, 416
MspCI CTTAAG 1 cut(s) 137
MspI CCGG 3 cut(s) 162, 288, 292
Mva1269I GAATGC 1 cut(s) 305
MvnI CGCG 1 cut(s) 108
NaeI GCCGGC 1 cut(s) 163
NdeII GATC 2 cut(s) 144, 762
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 4 cut(s) 55, 380, 725, 746
NlaIV GGNNCC 1 cut(s) 286
NmuCI GTSAC 2 cut(s) 130, 532
NruI TCGCGA 1 cut(s) 108
NspI RCATGY 1 cut(s) 725
OliI CACNNNNGTG 1 cut(s) 42
PceI AGGCCT 1 cut(s) 433
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PctI GAATGC 1 cut(s) 305
PdiI GCCGGC 1 cut(s) 163
PfeI GAWTC 1 cut(s) 103
PflFI GACNNNGTC 1 cut(s) 197
PflMI CCANNNNNTGG 1 cut(s) 521
PkrI GCNGC 3 cut(s) 31, 64, 727
Ppu21I YACGTR 1 cut(s) 484
PspN4I GGNNCC 1 cut(s) 286
PspPI GGNCC 1 cut(s) 285
PsrI GAACNNNNNNTAC 2 cut(s) 347, 379
PsyI GACNNNGTC 1 cut(s) 197
RruI TCGCGA 1 cut(s) 108
RsaI GTAC 5 cut(s) 256, 365, 482, 486, 702
RsaNI GTAC 5 cut(s) 255, 364, 481, 485, 701
RseI CAYNNNNRTG 2 cut(s) 42, 416
SaqAI TTAA 2 cut(s) 138, 216
SatI GCNGC 3 cut(s) 30, 63, 726
Sau3AI GATC 2 cut(s) 144, 762
Sau96I GGNCC 1 cut(s) 285
SfaNI GCATC 2 cut(s) 250, 649
SinI GGWCC 1 cut(s) 285
SmiMI CAYNNNNRTG 2 cut(s) 42, 416
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SnaBI TACGTA 1 cut(s) 484
Sse9I AATT 1 cut(s) 555
SseBI AGGCCT 1 cut(s) 433
SsiI CCGC 5 cut(s) 30, 42, 60, 63, 662
SspMI CTAG 1 cut(s) 729
StuI AGGCCT 1 cut(s) 433
TaaI ACNGT 1 cut(s) 214
TaiI ACGT 5 cut(s) 9, 114, 369, 486, 773
TaqI TCGA 6 cut(s) 18, 201, 342, 353, 471, 765
TasI AATT 1 cut(s) 555
TatI WGTACW 1 cut(s) 254
TauI GCSGC 2 cut(s) 32, 65
TfiI GAWTC 1 cut(s) 103
Tru1I TTAA 2 cut(s) 138, 216
Tru9I TTAA 2 cut(s) 138, 216
TscAI CASTG 5 cut(s) 126, 135, 217, 337, 711
TseFI GTSAC 2 cut(s) 130, 532
TseI GCWGC 1 cut(s) 725
Tsp45I GTSAC 2 cut(s) 130, 532
TspDTI ATGAA 3 cut(s) 17, 40, 365
TspGWI ACGGA 2 cut(s) 36, 372
TspRI CASTG 5 cut(s) 126, 135, 217, 337, 711
Tth111I GACNNNGTC 1 cut(s) 197
Van91I CCANNNNNTGG 1 cut(s) 521
Vha464I CTTAAG 1 cut(s) 137
VpaK11BI GGWCC 1 cut(s) 285
XceI RCATGY 1 cut(s) 725
XspI CTAG 1 cut(s) 729
ZraI GACGTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.