Rmu_sc0027415.1_g000001

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0027415.1
OG0000308
Physical Location & Seq
Reverse (-)
422 .. 1674
1253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0027415.1_g000001.1.cds

Sequence Viewer

Length: 1206 bp
atggcaattgatgcaatggcagcccgcttggcctcctccctagcgctggctgatggcaagcaaccggtggatttgcgtccatcaaaaggattgagacaacctgaagcattcatggtaggaaagctactcacggcacgggaatattctcgtcgatctttccttggcctgtttcgatctgcatggagaatcaatggcagattgaatgttgaagaactcggagaagatgatagggtcttgttcacttttacagacccttcggatgtgaatagggtttggaaaggaggaccatggggatataacagggctcctatagccttagctccatacgacggtgtcagtccggcaagggagatcccactgaagaaatcatcatattggataacactacaaggagtgccaccggccttcagatccgaatggattatgcgactgattgagagtaccctaggagagttcatggaagttgatcgccccgcctttcggacgcgagttttgcgcattagggtcgagatcgattatgtcaaacctctcctgtttcgtcgacgtttcagggtggaagatgaagtccagtcatggtgcagtttcgatatgacaaattgtttggcagggccggatctggatgaagaccgtgatatacccgttggcacggtggagcaacccacggctttagccgtattacggggactggaaaaccctaatgtcaatagggtaacgataaacaaccaaactctgattttcaaggctcaaatccctaatagcatacccgaagctatcctacctcaatctgtgatggccatgcgtcgacatcgaagacaagtgcagattagagaattgagtgaaccaagactcaatgaagatccaatcacaggacaacgccgtagtagagaacatgatgaggtaactatctctccaaaaaaattgcgtttaagtcttgctattggaaaaaaaaaattggatgcagtgactatgggattgttggagggacctccgaacaataatgatcaacctaagagaaagagaggacgtcctattggaagtgtgaacaagctaccatatggaacagcaggaaaaggaagaaaaaaggatggagaacaagaaaagataaagagcaaaaagaccaacctgatgacgaggttgttagctgctgatgaacctgaaacaagcactaactctaaccctaaggggaaagagcaagtgcttgcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

401

Amino Acids

45.32

Weight (kDa)

9.91

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Orthologous Genes (Group: OG0000308)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24891 FvH4_4g09031
rosa_multiflora Rmu_co8001542.1_g000001 Rmu_co8074240.1_g000001 Rmu_co8092076.1_g000001 Rmu_co8136936.1_g000001 Rmu_co8303129.1_g000001 Rmu_co8326579.1_g000001 Rmu_co8378475.1_g000001 Rmu_co8409011.1_g000001 Rmu_co8449663.1_g000001 Rmu_co8463085.1_g000001 Rmu_sc0000146.1_g000025 Rmu_sc0000294.1_g000018 Rmu_sc0000299.1_g000003 Rmu_sc0000342.1_g000003 Rmu_sc0000359.1_g000009 Rmu_sc0000431.1_g000022 Rmu_sc0000442.1_g000006 Rmu_sc0000458.1_g000011 Rmu_sc0000560.1_g000013 Rmu_sc0000594.1_g000075 Rmu_sc0000740.1_g000033 Rmu_sc0000839.1_g000002 Rmu_sc0000939.1_g000034 Rmu_sc0000955.1_g000002 Rmu_sc0001069.1_g000045 Rmu_sc0001074.1_g000019 Rmu_sc0001418.1_g000003 Rmu_sc0001484.1_g000006 Rmu_sc0001499.1_g000062 Rmu_sc0001631.1_g000002 Rmu_sc0001639.1_g000021 Rmu_sc0002146.1_g000007 Rmu_sc0002146.1_g000010 Rmu_sc0002260.1_g000065 Rmu_sc0002273.1_g000005 Rmu_sc0002310.1_g000016 Rmu_sc0002414.1_g000051 Rmu_sc0002479.1_g000003 Rmu_sc0002586.1_g000034 Rmu_sc0002586.1_g000035 Rmu_sc0002589.1_g000034 Rmu_sc0003187.1_g000020 Rmu_sc0003444.1_g000018 Rmu_sc0003633.1_g000041 Rmu_sc0003973.1_g000010 Rmu_sc0004209.1_g000004 Rmu_sc0004927.1_g000020 Rmu_sc0004950.1_g000007 Rmu_sc0004952.1_g000012 Rmu_sc0004966.1_g000001 Rmu_sc0006168.1_g000026 Rmu_sc0006325.1_g000003 Rmu_sc0006413.1_g000015 Rmu_sc0007192.1_g000002 Rmu_sc0007390.1_g000004 Rmu_sc0007847.1_g000003 Rmu_sc0008044.1_g000001 Rmu_sc0008101.1_g000008 Rmu_sc0008442.1_g000015 Rmu_sc0008714.1_g000004 Rmu_sc0009057.1_g000009 Rmu_sc0009916.1_g000015 Rmu_sc0010180.1_g000003 Rmu_sc0010560.1_g000005 Rmu_sc0010715.1_g000005 Rmu_sc0011022.1_g000002 Rmu_sc0011509.1_g000006 Rmu_sc0011831.1_g000006 Rmu_sc0012010.1_g000002 Rmu_sc0013320.1_g000007 Rmu_sc0013801.1_g000006 Rmu_sc0013801.1_g000007 Rmu_sc0013887.1_g000002 Rmu_sc0014485.1_g000014 Rmu_sc0018736.1_g000001 Rmu_sc0019355.1_g000001 Rmu_sc0020316.1_g000002 Rmu_sc0021214.1_g000001 Rmu_sc0021375.1_g000001 Rmu_sc0023412.1_g000001 Rmu_sc0024234.1_g000001 Rmu_sc0027415.1_g000001 Rmu_sc0029109.1_g000001 Rmu_sc0031708.1_g000001 Rmu_sc0035307.1_g000001 Rmu_sc0036546.1_g000001 Rmu_sc0037386.1_g000005 Rmu_sc0039204.1_g000001 Rmu_sc0039709.1_g000001 Rmu_sc0039867.1_g000001 Rmu_sc0040008.1_g000002 Rmu_sc0040009.1_g000002 Rmu_sc0041433.1_g000001 Rmu_sc0043076.1_g000001 Rmu_ssc0000024.1_g000022 Rmu_ssc0000065.1_g000003 Rmu_ssc0000100.1_g000036 Rmu_ssc0000171.1_g000015 Rmu_ssc0000190.1_g000018 Rmu_ssc0000226.1_g000017 Rmu_ssc0000244.1_g000039 Rmu_ssc0000419.1_g000008 Rmu_ssc0000451.1_g000014
rosa_roxburghii Rroxscaffold_1G00006150 Rroxscaffold_3G00219360 Rroxscaffold_5G00368320 Rroxscaffold_7G00191310
rosa_rugosa Rorug04G0060300 Rorug04G0115300 Rorug04G0128800
rosa_samantha Rh1AG029600 Rh2BG406100 Rh4DG128300 Rh5DG195500 Rh6AG427300 Rh6CG440900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1027
Acc16I TGCGCA 1 cut(s) 497
AccI GTMKAC 2 cut(s) 541, 802
AccII CGCG 1 cut(s) 487
AciI CCGC 2 cut(s) 25, 474
AclWI GGATC 4 cut(s) 346, 405, 621, 851
AcoI YGGCCR 1 cut(s) 792
AcuI CTGAAG 3 cut(s) 123, 380, 391
AcyI GRCGYC 1 cut(s) 1024
AfaI GTAC 1 cut(s) 442
AfeI AGCGCT 1 cut(s) 45
AfiI CCNNNNNNNGG 6 cut(s) 46, 86, 329, 480, 678, 866
AgeI ACCGGT 1 cut(s) 64
AgsI TTSAA 3 cut(s) 202, 209, 739
AluBI AGCT 5 cut(s) 124, 320, 770, 1048, 1142
AluI AGCT 5 cut(s) 124, 320, 770, 1048, 1142
Alw26I GTCTC 1 cut(s) 88
AlwI GGATC 4 cut(s) 346, 405, 621, 851
Aor51HI AGCGCT 1 cut(s) 45
AoxI GGCC 5 cut(s) 30, 163, 402, 608, 792
ApeKI GCWGC 2 cut(s) 20, 1142
ArsI GACNNNNNNTTYG 4 cut(s) 475, 507, 583, 615
AsiGI ACCGGT 1 cut(s) 64
AspA2I CCTAGG 1 cut(s) 445
AspLEI GCGC 2 cut(s) 46, 498
AspS9I GGNCC 3 cut(s) 284, 608, 983
AvaII GGWCC 2 cut(s) 284, 983
AvrII CCTAGG 1 cut(s) 445
AxyI CCTNAGG 1 cut(s) 1179
BalI TGGCCA 1 cut(s) 794
BanII GRGCYC 1 cut(s) 307
BarI GAAGNNNNNNTAC 2 cut(s) 759, 791
BbsI GAAGAC 2 cut(s) 630, 817
BbvI GCAGC 2 cut(s) 32, 1129
BccI CCATC 4 cut(s) 47, 88, 784, 1079
BceAI ACGGC 4 cut(s) 147, 656, 678, 861
BcgI CGANNNNNNTGC 2 cut(s) 487, 521
BclI TGATCA 1 cut(s) 1000
BcoDI GTCTC 1 cut(s) 88
BfaI CTAG 2 cut(s) 41, 446
BfmI CTRYAG 1 cut(s) 309
BfoI RGCGCY 1 cut(s) 47
BglI GCCNNNNNGGC 1 cut(s) 29
BisI GCNGC 2 cut(s) 21, 1143
BlnI CCTAGG 1 cut(s) 445
BlsI GCNGC 2 cut(s) 22, 1144
Bme18I GGWCC 2 cut(s) 284, 983
BmgT120I GGNCC 3 cut(s) 284, 608, 983
BmiI GGNNCC 2 cut(s) 306, 984
BmsI GCATC 1 cut(s) 946
BpiI GAAGAC 2 cut(s) 630, 817
Bpu10I CCTNAGC 1 cut(s) 316
Bsa29I ATCGAT 1 cut(s) 513
BsaHI GRCGYC 1 cut(s) 1024
BsaJI CCNNGG 4 cut(s) 160, 287, 445, 660
BsaWI WCCGGW 1 cut(s) 64
BsaXI ACNNNNNCTCC 2 cut(s) 892, 922
Bsc4I CCNNNNNNNGG 6 cut(s) 46, 86, 329, 480, 678, 866
Bse118I RCCGGY 2 cut(s) 64, 400
Bse1I ACTGG 2 cut(s) 568, 690
Bse21I CCTNAGG 1 cut(s) 1179
Bse3DI GCAATG 1 cut(s) 21
BseCI ATCGAT 1 cut(s) 513
BseDI CCNNGG 4 cut(s) 160, 287, 445, 660
BseGI GGATG 4 cut(s) 265, 625, 961, 1090
BseLI CCNNNNNNNGG 6 cut(s) 46, 86, 329, 480, 678, 866
BseMI GCAATG 1 cut(s) 21
BseNI ACTGG 2 cut(s) 568, 690
BseRI GAGGAG 1 cut(s) 25
BseXI GCAGC 2 cut(s) 32, 1129
BsgI GTGCAG 2 cut(s) 598, 839
Bsh1236I CGCG 1 cut(s) 487
BshFI GGCC 5 cut(s) 32, 165, 404, 610, 794
BshTI ACCGGT 1 cut(s) 64
BshVI ATCGAT 1 cut(s) 513
BsiSI CCGG 4 cut(s) 65, 341, 401, 611
BslFI GGGAC 2 cut(s) 696, 996
BslI CCNNNNNNNGG 6 cut(s) 46, 86, 329, 480, 678, 866
BsmAI GTCTC 1 cut(s) 88
BsmFI GGGAC 2 cut(s) 696, 996
BsmI GAATGC 1 cut(s) 107
BsnI GGCC 5 cut(s) 32, 165, 404, 610, 794
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 9 cut(s) 152, 173, 351, 410, 466, 510, 613, 856, 1000
Bsp19I CCATGG 1 cut(s) 287
BspACI CCGC 2 cut(s) 25, 474
BspANI GGCC 5 cut(s) 32, 165, 404, 610, 794
BspDI ATCGAT 1 cut(s) 513
BspFNI CGCG 1 cut(s) 487
BspLI GGNNCC 2 cut(s) 306, 984
BspPI GGATC 4 cut(s) 346, 405, 621, 851
BsrDI GCAATG 1 cut(s) 21
BsrFI RCCGGY 2 cut(s) 64, 400
BsrI ACTGG 2 cut(s) 568, 690
BssAI RCCGGY 2 cut(s) 64, 400
BssECI CCNNGG 4 cut(s) 160, 287, 445, 660
BssMI GATC 9 cut(s) 152, 173, 351, 410, 466, 510, 613, 856, 1000
BssNI GRCGYC 1 cut(s) 1024
BssT1I CCWWGG 3 cut(s) 160, 287, 445
Bst4CI ACNGT 3 cut(s) 332, 629, 649
BstACI GRCGYC 1 cut(s) 1024
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 4 cut(s) 25, 48, 59, 1200
BstDEI CTNAG 3 cut(s) 316, 1008, 1179
BstDSI CCRYGG 2 cut(s) 287, 660
BstF5I GGATG 4 cut(s) 265, 625, 961, 1090
BstFNI CGCG 1 cut(s) 487
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 2 cut(s) 46, 498
BstKTI GATC 9 cut(s) 155, 176, 354, 413, 469, 513, 616, 859, 1003
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 9 cut(s) 152, 173, 351, 410, 466, 510, 613, 856, 1000
BstMWI GCNNNNNNNGC 5 cut(s) 11, 20, 29, 311, 493
BstSFI CTRYAG 1 cut(s) 309
BstUI CGCG 1 cut(s) 487
BstV1I GCAGC 2 cut(s) 32, 1129
BstV2I GAAGAC 2 cut(s) 630, 817
BstX2I RGATCY 4 cut(s) 351, 410, 613, 856
BstYI RGATCY 4 cut(s) 351, 410, 613, 856
Bsu15I ATCGAT 1 cut(s) 513
Bsu36I CCTNAGG 1 cut(s) 1179
BsuRI GGCC 5 cut(s) 32, 165, 404, 610, 794
BsuTUI ATCGAT 1 cut(s) 513
BtgI CCRYGG 2 cut(s) 287, 660
BtsCI GGATG 4 cut(s) 265, 625, 961, 1090
BtsI GCAGTG 1 cut(s) 966
BtsIMutI CAGTG 2 cut(s) 356, 966
Cac8I GCNNGC 4 cut(s) 25, 48, 59, 1200
CfoI GCGC 2 cut(s) 46, 498
Cfr10I RCCGGY 2 cut(s) 64, 400
Cfr13I GGNCC 3 cut(s) 284, 608, 983
ClaI ATCGAT 1 cut(s) 513
CseI GACGC 3 cut(s) 65, 493, 788
Csp6I GTAC 1 cut(s) 441
CspAI ACCGGT 1 cut(s) 64
CviAII CATG 7 cut(s) 112, 180, 288, 457, 573, 796, 890
CviQI GTAC 1 cut(s) 441
DdeI CTNAG 3 cut(s) 316, 1008, 1179
DpnI GATC 9 cut(s) 154, 175, 353, 412, 468, 512, 615, 858, 1002
DpnII GATC 9 cut(s) 152, 173, 351, 410, 466, 510, 613, 856, 1000
EaeI YGGCCR 1 cut(s) 792
Eco130I CCWWGG 3 cut(s) 160, 287, 445
Eco24I GRGCYC 1 cut(s) 307
Eco47I GGWCC 2 cut(s) 284, 983
Eco47III AGCGCT 1 cut(s) 45
Eco57I CTGAAG 3 cut(s) 123, 380, 391
Eco81I CCTNAGG 1 cut(s) 1179
EcoO109I RGGNCCY 1 cut(s) 983
EcoT14I CCWWGG 3 cut(s) 160, 287, 445
EcoT38I GRGCYC 1 cut(s) 307
ErhI CCWWGG 3 cut(s) 160, 287, 445
FaeI CATG 7 cut(s) 115, 183, 291, 460, 576, 799, 893
FaqI GGGAC 2 cut(s) 696, 996
FatI CATG 7 cut(s) 111, 179, 287, 456, 572, 795, 889
FauI CCCGC 2 cut(s) 32, 481
FauNDI CATATG 1 cut(s) 1054
FbaI TGATCA 1 cut(s) 1000
FblI GTMKAC 2 cut(s) 541, 802
Fnu4HI GCNGC 2 cut(s) 21, 1143
FokI GGATG 4 cut(s) 272, 632, 968, 1097
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 2 cut(s) 21, 1143
FspBI CTAG 2 cut(s) 41, 446
FspI TGCGCA 1 cut(s) 497
GlaI GCGC 2 cut(s) 45, 497
GluI GCNGC 2 cut(s) 21, 1143
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 5 cut(s) 32, 165, 404, 610, 794
HapII CCGG 4 cut(s) 65, 341, 401, 611
HgaI GACGC 3 cut(s) 65, 493, 788
HhaI GCGC 2 cut(s) 46, 498
Hin1I GRCGYC 1 cut(s) 1024
Hin1II CATG 7 cut(s) 115, 183, 291, 460, 576, 799, 893
Hin6I GCGC 2 cut(s) 44, 496
HinP1I GCGC 2 cut(s) 44, 496
HincII GTYRAC 2 cut(s) 542, 803
HindII GTYRAC 2 cut(s) 542, 803
HinfI GANTC 2 cut(s) 186, 846
HpaII CCGG 4 cut(s) 65, 341, 401, 611
Hpy166II GTNNAC 5 cut(s) 240, 542, 803, 839, 1042
Hpy188I TCNGA 7 cut(s) 218, 259, 410, 415, 483, 732, 990
Hpy188III TCNNGA 2 cut(s) 508, 617
Hpy8I GTNNAC 5 cut(s) 240, 542, 803, 839, 1042
Hpy99I CGWCG 5 cut(s) 153, 332, 543, 546, 804
HpyAV CCTTC 2 cut(s) 264, 415
HpyCH4III ACNGT 3 cut(s) 332, 629, 649
HpyCH4IV ACGT 2 cut(s) 544, 1024
HpyCH4V TGCA 5 cut(s) 14, 179, 579, 820, 959
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 20, 29, 311, 493
HpyF3I CTNAG 3 cut(s) 316, 1008, 1179
HpySE526I ACGT 2 cut(s) 544, 1024
Hsp92I GRCGYC 1 cut(s) 1024
Hsp92II CATG 7 cut(s) 115, 183, 291, 460, 576, 799, 893
HspAI GCGC 2 cut(s) 44, 496
Ksp22I TGATCA 1 cut(s) 1000
Kzo9I GATC 9 cut(s) 152, 173, 351, 410, 466, 510, 613, 856, 1000
LmnI GCTCC 3 cut(s) 310, 325, 652
Lsp1109I GCAGC 2 cut(s) 32, 1129
LweI GCATC 1 cut(s) 946
MaeI CTAG 2 cut(s) 41, 446
MaeII ACGT 2 cut(s) 544, 1024
MaeIII GTNAC 3 cut(s) 709, 898, 961
MalI GATC 9 cut(s) 154, 175, 353, 412, 468, 512, 615, 858, 1002
MboI GATC 9 cut(s) 152, 173, 351, 410, 466, 510, 613, 856, 1000
MboII GAAGA 8 cut(s) 221, 233, 373, 569, 635, 822, 866, 1086
MfeI CAATTG 1 cut(s) 6
MflI RGATCY 4 cut(s) 351, 410, 613, 856
MhlI GDGCHC 1 cut(s) 307
MlsI TGGCCA 1 cut(s) 794
MluCI AATT 5 cut(s) 6, 595, 830, 917, 950
MluNI TGGCCA 1 cut(s) 794
MlyI GAGTC 1 cut(s) 840
MmeI TCCRAC 1 cut(s) 957
Mox20I TGGCCA 1 cut(s) 794
MscI TGGCCA 1 cut(s) 794
MseI TTAA 2 cut(s) 926, 1204
Msp20I TGGCCA 1 cut(s) 794
MspI CCGG 4 cut(s) 65, 341, 401, 611
MunI CAATTG 1 cut(s) 6
Mva1269I GAATGC 1 cut(s) 107
MvnI CGCG 1 cut(s) 487
MwoI GCNNNNNNNGC 5 cut(s) 11, 20, 29, 311, 493
NcoI CCATGG 1 cut(s) 287
NdeI CATATG 1 cut(s) 1054
NdeII GATC 9 cut(s) 152, 173, 351, 410, 466, 510, 613, 856, 1000
NlaIII CATG 7 cut(s) 115, 183, 291, 460, 576, 799, 893
NlaIV GGNNCC 2 cut(s) 306, 984
NmuCI GTSAC 1 cut(s) 961
NsbI TGCGCA 1 cut(s) 497
PctI GAATGC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 186
PflFI GACNNNGTC 1 cut(s) 332
PinAI ACCGGT 1 cut(s) 64
PkrI GCNGC 2 cut(s) 22, 1144
PleI GAGTC 1 cut(s) 840
PpsI GAGTC 1 cut(s) 840
PpuMI RGGWCCY 1 cut(s) 983
Psp5II RGGWCCY 1 cut(s) 983
PspN4I GGNNCC 2 cut(s) 306, 984
PspPI GGNCC 3 cut(s) 284, 608, 983
PspPPI RGGWCCY 1 cut(s) 983
PsuI RGATCY 4 cut(s) 351, 410, 613, 856
PsyI GACNNNGTC 1 cut(s) 332
RsaI GTAC 1 cut(s) 442
RsaNI GTAC 1 cut(s) 441
SalI GTCGAC 2 cut(s) 540, 801
SaqAI TTAA 2 cut(s) 926, 1204
SatI GCNGC 2 cut(s) 21, 1143
Sau3AI GATC 9 cut(s) 152, 173, 351, 410, 466, 510, 613, 856, 1000
Sau96I GGNCC 3 cut(s) 284, 608, 983
SchI GAGTC 1 cut(s) 840
SduI GDGCHC 1 cut(s) 307
SfaNI GCATC 1 cut(s) 946
SfcI CTRYAG 1 cut(s) 309
SgrDI CGTCGACG 1 cut(s) 540
SinI GGWCC 2 cut(s) 284, 983
Sse9I AATT 5 cut(s) 6, 595, 830, 917, 950
SsiI CCGC 2 cut(s) 25, 474
SspI AATATT 1 cut(s) 143
SspMI CTAG 2 cut(s) 41, 446
StyI CCWWGG 3 cut(s) 160, 287, 445
TaaI ACNGT 3 cut(s) 332, 629, 649
TaiI ACGT 2 cut(s) 547, 1027
TaqI TCGA 8 cut(s) 151, 172, 507, 513, 541, 585, 802, 808
TasI AATT 5 cut(s) 6, 595, 830, 917, 950
TfiI GAWTC 1 cut(s) 186
Tru1I TTAA 2 cut(s) 926, 1204
Tru9I TTAA 2 cut(s) 926, 1204
TscAI CASTG 2 cut(s) 363, 966
TseFI GTSAC 1 cut(s) 961
TseI GCWGC 2 cut(s) 20, 1142
Tsp45I GTSAC 1 cut(s) 961
TspDTI ATGAA 6 cut(s) 100, 445, 576, 636, 867, 1164
TspRI CASTG 2 cut(s) 363, 966
Tth111I GACNNNGTC 1 cut(s) 332
VpaK11BI GGWCC 2 cut(s) 284, 983
XmaJI CCTAGG 1 cut(s) 445
XmiI GTMKAC 2 cut(s) 541, 802
XspI CTAG 2 cut(s) 41, 446
ZraI GACGTC 1 cut(s) 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.