Rmu_sc0021214.1_g000001

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0021214.1
OG0000308
Physical Location & Seq
Reverse (-)
191 .. 1349
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0021214.1_g000001.1.cds

Sequence Viewer

Length: 933 bp
atgcttgggaagttactgactgccagggattatacacgctggtctttcatggggatgttcagatctgcgtggaggattaatggcactcttcgtgtcgacgaggcgggagaagatgattgtgttttgttttctttttcggatccaatcgacagggctagggtttggaaaggcggtccgtggggtttcaatcgagccccgatagagctggccgagtatgatggaatgagccctgtccagtttgttgtaaagttcaaatacgacaagttatttggctggtgtacccgctgtgggctggttactcatgtgggattgccatgctccgggccggagttgctggaagatgatcagggaccagtagggacagtggagcctccatcaaccttggcggtgttgcgtggtgggctggaaccaagtgcaattgcacctagggttttggcaaatggccaaaccctagttttcacagcccaggttcctcgagctattccagaagtcctcctcccgcagaacgctgtgaacatacaccaagttaaaaggccggtgcagattagggaacttgaggagccacgacaggaggtactagccattacaggggtgcgtcgtcaccacgaaggagaggaggtactcatctctcctaagaagcttcgtctcagtttggctataggtaaaaaaaatgtagatgcagtgagtatgggtcttgttgaagcacatactagtgctacggagcagccaaagaggaagcgttgtcgtcctgtaggcaatctcaacaaaatgccatatggtactgcaggaaaaaacaaaatggtaaaaaagaatgaggaccaaggtaagcagaacaagaggaataacaagaagaccaacctgatgtcgaggttgttagctgctgaagatggagctaattccaaccctaaggggaaggagttggctcttatctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

310

Amino Acids

34.34

Weight (kDa)

9.55

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000308)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24891 FvH4_4g09031
rosa_multiflora Rmu_co8001542.1_g000001 Rmu_co8074240.1_g000001 Rmu_co8092076.1_g000001 Rmu_co8136936.1_g000001 Rmu_co8303129.1_g000001 Rmu_co8326579.1_g000001 Rmu_co8378475.1_g000001 Rmu_co8409011.1_g000001 Rmu_co8449663.1_g000001 Rmu_co8463085.1_g000001 Rmu_sc0000146.1_g000025 Rmu_sc0000294.1_g000018 Rmu_sc0000299.1_g000003 Rmu_sc0000342.1_g000003 Rmu_sc0000359.1_g000009 Rmu_sc0000431.1_g000022 Rmu_sc0000442.1_g000006 Rmu_sc0000458.1_g000011 Rmu_sc0000560.1_g000013 Rmu_sc0000594.1_g000075 Rmu_sc0000740.1_g000033 Rmu_sc0000839.1_g000002 Rmu_sc0000939.1_g000034 Rmu_sc0000955.1_g000002 Rmu_sc0001069.1_g000045 Rmu_sc0001074.1_g000019 Rmu_sc0001418.1_g000003 Rmu_sc0001484.1_g000006 Rmu_sc0001499.1_g000062 Rmu_sc0001631.1_g000002 Rmu_sc0001639.1_g000021 Rmu_sc0002146.1_g000007 Rmu_sc0002146.1_g000010 Rmu_sc0002260.1_g000065 Rmu_sc0002273.1_g000005 Rmu_sc0002310.1_g000016 Rmu_sc0002414.1_g000051 Rmu_sc0002479.1_g000003 Rmu_sc0002586.1_g000034 Rmu_sc0002586.1_g000035 Rmu_sc0002589.1_g000034 Rmu_sc0003187.1_g000020 Rmu_sc0003444.1_g000018 Rmu_sc0003633.1_g000041 Rmu_sc0003973.1_g000010 Rmu_sc0004209.1_g000004 Rmu_sc0004927.1_g000020 Rmu_sc0004950.1_g000007 Rmu_sc0004952.1_g000012 Rmu_sc0004966.1_g000001 Rmu_sc0006168.1_g000026 Rmu_sc0006325.1_g000003 Rmu_sc0006413.1_g000015 Rmu_sc0007192.1_g000002 Rmu_sc0007390.1_g000004 Rmu_sc0007847.1_g000003 Rmu_sc0008044.1_g000001 Rmu_sc0008101.1_g000008 Rmu_sc0008442.1_g000015 Rmu_sc0008714.1_g000004 Rmu_sc0009057.1_g000009 Rmu_sc0009916.1_g000015 Rmu_sc0010180.1_g000003 Rmu_sc0010560.1_g000005 Rmu_sc0010715.1_g000005 Rmu_sc0011022.1_g000002 Rmu_sc0011509.1_g000006 Rmu_sc0011831.1_g000006 Rmu_sc0012010.1_g000002 Rmu_sc0013320.1_g000007 Rmu_sc0013801.1_g000006 Rmu_sc0013801.1_g000007 Rmu_sc0013887.1_g000002 Rmu_sc0014485.1_g000014 Rmu_sc0018736.1_g000001 Rmu_sc0019355.1_g000001 Rmu_sc0020316.1_g000002 Rmu_sc0021214.1_g000001 Rmu_sc0021375.1_g000001 Rmu_sc0023412.1_g000001 Rmu_sc0024234.1_g000001 Rmu_sc0027415.1_g000001 Rmu_sc0029109.1_g000001 Rmu_sc0031708.1_g000001 Rmu_sc0035307.1_g000001 Rmu_sc0036546.1_g000001 Rmu_sc0037386.1_g000005 Rmu_sc0039204.1_g000001 Rmu_sc0039709.1_g000001 Rmu_sc0039867.1_g000001 Rmu_sc0040008.1_g000002 Rmu_sc0040009.1_g000002 Rmu_sc0041433.1_g000001 Rmu_sc0043076.1_g000001 Rmu_ssc0000024.1_g000022 Rmu_ssc0000065.1_g000003 Rmu_ssc0000100.1_g000036 Rmu_ssc0000171.1_g000015 Rmu_ssc0000190.1_g000018 Rmu_ssc0000226.1_g000017 Rmu_ssc0000244.1_g000039 Rmu_ssc0000419.1_g000008 Rmu_ssc0000451.1_g000014
rosa_roxburghii Rroxscaffold_1G00006150 Rroxscaffold_3G00219360 Rroxscaffold_5G00368320 Rroxscaffold_7G00191310
rosa_rugosa Rorug04G0060300 Rorug04G0115300 Rorug04G0128800
rosa_samantha Rh1AG029600 Rh2BG406100 Rh4DG128300 Rh5DG195500 Rh6AG427300 Rh6CG440900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 96
AciI CCGC 5 cut(s) 104, 171, 283, 386, 500
AclWI GGATC 2 cut(s) 134, 147
AcoI YGGCCR 2 cut(s) 207, 442
AcuI CTGAAG 1 cut(s) 903
AfaI GTAC 4 cut(s) 280, 576, 621, 781
AfiI CCNNNNNNNGG 3 cut(s) 288, 320, 588
AgsI TTSAA 3 cut(s) 187, 253, 701
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 2 cut(s) 23, 465
AluBI AGCT 5 cut(s) 205, 479, 640, 878, 893
AluI AGCT 5 cut(s) 205, 479, 640, 878, 893
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 2 cut(s) 134, 147
Ama87I CYCGRG 1 cut(s) 474
AoxI GGCC 4 cut(s) 207, 323, 442, 533
ApeKI GCWGC 2 cut(s) 724, 878
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
AseI ATTAAT 1 cut(s) 78
AspA2I CCTAGG 1 cut(s) 425
AspS9I GGNCC 4 cut(s) 173, 323, 350, 817
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 593
AvaI CYCGRG 1 cut(s) 474
AvaII GGWCC 3 cut(s) 173, 350, 817
AvrII CCTAGG 1 cut(s) 425
AxyI CCTNAGG 1 cut(s) 906
BalI TGGCCA 1 cut(s) 444
BamHI GGATCC 1 cut(s) 139
BanII GRGCYC 2 cut(s) 196, 230
BbsI GAAGAC 1 cut(s) 857
BbvI GCAGC 2 cut(s) 736, 865
BccI CCATC 3 cut(s) 212, 382, 881
BciT130I CCWGG 2 cut(s) 25, 467
BclI TGATCA 1 cut(s) 343
BcnI CCSGG 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 650
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 6 cut(s) 156, 426, 452, 578, 711, 931
BfmI CTRYAG 3 cut(s) 657, 750, 783
BglII AGATCT 1 cut(s) 62
BisI GCNGC 2 cut(s) 725, 879
BlnI CCTAGG 1 cut(s) 425
BlsI GCNGC 2 cut(s) 726, 880
Bme1390I CCNGG 3 cut(s) 25, 322, 467
Bme18I GGWCC 3 cut(s) 173, 350, 817
BmeT110I CYCGRG 1 cut(s) 474
BmgT120I GGNCC 4 cut(s) 173, 323, 350, 817
BmiI GGNNCC 6 cut(s) 141, 351, 369, 408, 471, 561
BmrFI CCNGG 3 cut(s) 25, 322, 467
BmsI GCATC 1 cut(s) 667
BpiI GAAGAC 1 cut(s) 857
BpuEI CTTGAG 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 322
BsaJI CCNNGG 6 cut(s) 24, 176, 381, 425, 465, 820
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 3 cut(s) 288, 320, 588
Bse118I RCCGGY 1 cut(s) 535
Bse1I ACTGG 2 cut(s) 235, 353
Bse21I CCTNAGG 1 cut(s) 906
BseBI CCWGG 2 cut(s) 25, 467
BseDI CCNNGG 6 cut(s) 24, 176, 381, 425, 465, 820
BseGI GGATG 1 cut(s) 60
BseLI CCNNNNNNNGG 3 cut(s) 288, 320, 588
BseMII CTCAG 1 cut(s) 661
BseNI ACTGG 2 cut(s) 235, 353
BseRI GAGGAG 3 cut(s) 485, 572, 629
BseXI GCAGC 2 cut(s) 736, 865
BsgI GTGCAG 1 cut(s) 560
BshFI GGCC 4 cut(s) 209, 325, 444, 535
BsiHKCI CYCGRG 1 cut(s) 474
BsiSI CCGG 3 cut(s) 321, 326, 536
BslFI GGGAC 2 cut(s) 363, 373
BslI CCNNNNNNNGG 3 cut(s) 288, 320, 588
BsmAI GTCTC 1 cut(s) 650
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 2 cut(s) 363, 373
BsnI GGCC 4 cut(s) 209, 325, 444, 535
BsoBI CYCGRG 1 cut(s) 474
Bsp1286I GDGCHC 2 cut(s) 196, 230
Bsp143I GATC 3 cut(s) 62, 139, 343
BspACI CCGC 5 cut(s) 104, 171, 283, 386, 500
BspANI GGCC 4 cut(s) 209, 325, 444, 535
BspCNI CTCAG 1 cut(s) 660
BspLI GGNNCC 6 cut(s) 141, 351, 369, 408, 471, 561
BspMAI CTGCAG 1 cut(s) 787
BspPI GGATC 2 cut(s) 134, 147
BsrFI RCCGGY 1 cut(s) 535
BsrI ACTGG 2 cut(s) 235, 353
BssAI RCCGGY 1 cut(s) 535
BssECI CCNNGG 6 cut(s) 24, 176, 381, 425, 465, 820
BssMI GATC 3 cut(s) 62, 139, 343
BssT1I CCWWGG 3 cut(s) 381, 425, 820
Bst2UI CCWGG 2 cut(s) 25, 467
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 1 cut(s) 93
BstC8I GCNNGC 1 cut(s) 207
BstDEI CTNAG 3 cut(s) 633, 647, 906
BstDSI CCRYGG 1 cut(s) 176
BstF5I GGATG 1 cut(s) 60
BstKTI GATC 3 cut(s) 65, 142, 346
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 3 cut(s) 62, 139, 343
BstMWI GCNNNNNNNGC 2 cut(s) 331, 400
BstNI CCWGG 2 cut(s) 25, 467
BstSCI CCNGG 3 cut(s) 23, 320, 465
BstSFI CTRYAG 3 cut(s) 657, 750, 783
BstV1I GCAGC 2 cut(s) 736, 865
BstV2I GAAGAC 1 cut(s) 857
BstX2I RGATCY 2 cut(s) 62, 139
BstYI RGATCY 2 cut(s) 62, 139
Bsu36I CCTNAGG 1 cut(s) 906
BsuRI GGCC 4 cut(s) 209, 325, 444, 535
BtgI CCRYGG 1 cut(s) 176
BtsCI GGATG 1 cut(s) 60
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 2 cut(s) 369, 687
Cac8I GCNNGC 1 cut(s) 207
Cfr10I RCCGGY 1 cut(s) 535
Cfr13I GGNCC 4 cut(s) 173, 323, 350, 817
CpoI CGGWCCG 1 cut(s) 173
CseI GACGC 1 cut(s) 584
Csp6I GTAC 4 cut(s) 279, 575, 620, 780
CspI CGGWCCG 1 cut(s) 173
CviAII CATG 3 cut(s) 49, 302, 315
CviQI GTAC 4 cut(s) 279, 575, 620, 780
DdeI CTNAG 3 cut(s) 633, 647, 906
DpnI GATC 3 cut(s) 64, 141, 345
DpnII GATC 3 cut(s) 62, 139, 343
EaeI YGGCCR 2 cut(s) 207, 442
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Eco130I CCWWGG 3 cut(s) 381, 425, 820
Eco24I GRGCYC 2 cut(s) 196, 230
Eco47I GGWCC 3 cut(s) 173, 350, 817
Eco57I CTGAAG 1 cut(s) 903
Eco81I CCTNAGG 1 cut(s) 906
Eco88I CYCGRG 1 cut(s) 474
EcoRII CCWGG 2 cut(s) 23, 465
EcoT14I CCWWGG 3 cut(s) 381, 425, 820
EcoT38I GRGCYC 2 cut(s) 196, 230
ErhI CCWWGG 3 cut(s) 381, 425, 820
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 3 cut(s) 52, 305, 318
FaqI GGGAC 2 cut(s) 363, 373
FatI CATG 3 cut(s) 48, 301, 314
FauI CCCGC 3 cut(s) 97, 290, 507
FauNDI CATATG 1 cut(s) 775
FbaI TGATCA 1 cut(s) 343
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 2 cut(s) 725, 879
FokI GGATG 1 cut(s) 67
FriOI GRGCYC 2 cut(s) 196, 230
Fsp4HI GCNGC 2 cut(s) 725, 879
FspBI CTAG 6 cut(s) 156, 426, 452, 578, 711, 931
GluI GCNGC 2 cut(s) 725, 879
HaeIII GGCC 4 cut(s) 209, 325, 444, 535
HapII CCGG 3 cut(s) 321, 326, 536
HgaI GACGC 1 cut(s) 584
Hin1II CATG 3 cut(s) 52, 305, 318
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HindIII AAGCTT 1 cut(s) 638
HpaII CCGG 3 cut(s) 321, 326, 536
HphI GGTGA 1 cut(s) 593
Hpy166II GTNNAC 3 cut(s) 97, 279, 514
Hpy188I TCNGA 2 cut(s) 62, 139
Hpy188III TCNNGA 1 cut(s) 485
Hpy8I GTNNAC 3 cut(s) 97, 279, 514
Hpy99I CGWCG 2 cut(s) 101, 600
HpyAV CCTTC 2 cut(s) 602, 907
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4V TGCA 5 cut(s) 416, 422, 541, 680, 785
HpyF10VI GCNNNNNNNGC 2 cut(s) 331, 400
HpyF3I CTNAG 3 cut(s) 633, 647, 906
Hsp92II CATG 3 cut(s) 52, 305, 318
Ksp22I TGATCA 1 cut(s) 343
Kzo9I GATC 3 cut(s) 62, 139, 343
LmnI GCTCC 5 cut(s) 323, 367, 559, 721, 890
Lsp1109I GCAGC 2 cut(s) 736, 865
LweI GCATC 1 cut(s) 667
MaeI CTAG 6 cut(s) 156, 426, 452, 578, 711, 931
MaeIII GTNAC 3 cut(s) 12, 295, 599
MalI GATC 3 cut(s) 64, 141, 345
MboI GATC 3 cut(s) 62, 139, 343
MboII GAAGA 5 cut(s) 80, 122, 350, 862, 896
MfeI CAATTG 1 cut(s) 417
MflI RGATCY 2 cut(s) 62, 139
MhlI GDGCHC 2 cut(s) 196, 230
MlsI TGGCCA 1 cut(s) 444
MluCI AATT 2 cut(s) 417, 895
MluNI TGGCCA 1 cut(s) 444
MmeI TCCRAC 1 cut(s) 924
Mox20I TGGCCA 1 cut(s) 444
MscI TGGCCA 1 cut(s) 444
MseI TTAA 2 cut(s) 78, 528
MslI CAYNNNNRTG 2 cut(s) 53, 711
Msp20I TGGCCA 1 cut(s) 444
MspA1I CMGCKG 1 cut(s) 285
MspI CCGG 3 cut(s) 321, 326, 536
MspR9I CCNGG 3 cut(s) 25, 322, 467
MunI CAATTG 1 cut(s) 417
MvaI CCWGG 2 cut(s) 25, 467
MwoI GCNNNNNNNGC 2 cut(s) 331, 400
NciI CCSGG 1 cut(s) 322
NdeI CATATG 1 cut(s) 775
NdeII GATC 3 cut(s) 62, 139, 343
NlaIII CATG 3 cut(s) 52, 305, 318
NlaIV GGNNCC 6 cut(s) 141, 351, 369, 408, 471, 561
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 1 cut(s) 599
PaeR7I CTCGAG 1 cut(s) 474
PkrI GCNGC 2 cut(s) 726, 880
PshBI ATTAAT 1 cut(s) 78
Psp6I CCWGG 2 cut(s) 23, 465
PspGI CCWGG 2 cut(s) 23, 465
PspN4I GGNNCC 6 cut(s) 141, 351, 369, 408, 471, 561
PspPI GGNCC 4 cut(s) 173, 323, 350, 817
PspXI VCTCGAGB 1 cut(s) 474
PstI CTGCAG 1 cut(s) 787
PsuI RGATCY 2 cut(s) 62, 139
RsaI GTAC 4 cut(s) 280, 576, 621, 781
RsaNI GTAC 4 cut(s) 279, 575, 620, 780
RseI CAYNNNNRTG 2 cut(s) 53, 711
Rsr2I CGGWCCG 1 cut(s) 173
RsrII CGGWCCG 1 cut(s) 173
SalI GTCGAC 1 cut(s) 95
SaqAI TTAA 2 cut(s) 78, 528
SatI GCNGC 2 cut(s) 725, 879
Sau3AI GATC 3 cut(s) 62, 139, 343
Sau96I GGNCC 4 cut(s) 173, 323, 350, 817
ScrFI CCNGG 3 cut(s) 25, 322, 467
SduI GDGCHC 2 cut(s) 196, 230
SfaNI GCATC 1 cut(s) 667
SfcI CTRYAG 3 cut(s) 657, 750, 783
Sfr274I CTCGAG 1 cut(s) 474
SinI GGWCC 3 cut(s) 173, 350, 817
SlaI CTCGAG 1 cut(s) 474
SmiMI CAYNNNNRTG 2 cut(s) 53, 711
SmlI CTYRAG 2 cut(s) 474, 554
SmoI CTYRAG 2 cut(s) 474, 554
SpeI ACTAGT 1 cut(s) 710
Sse9I AATT 2 cut(s) 417, 895
SsiI CCGC 5 cut(s) 104, 171, 283, 386, 500
SspMI CTAG 6 cut(s) 156, 426, 452, 578, 711, 931
StyD4I CCNGG 3 cut(s) 23, 320, 465
StyI CCWWGG 3 cut(s) 381, 425, 820
TaaI ACNGT 1 cut(s) 364
TaqI TCGA 5 cut(s) 96, 147, 190, 475, 866
TasI AATT 2 cut(s) 417, 895
Tru1I TTAA 2 cut(s) 78, 528
Tru9I TTAA 2 cut(s) 78, 528
TscAI CASTG 2 cut(s) 369, 687
TseFI GTSAC 1 cut(s) 599
TseI GCWGC 2 cut(s) 724, 878
Tsp45I GTSAC 1 cut(s) 599
TspDTI ATGAA 1 cut(s) 37
TspGWI ACGGA 2 cut(s) 165, 734
TspRI CASTG 2 cut(s) 369, 687
VpaK11BI GGWCC 3 cut(s) 173, 350, 817
VspI ATTAAT 1 cut(s) 78
XhoI CTCGAG 1 cut(s) 474
XmaJI CCTAGG 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 96
XspI CTAG 6 cut(s) 156, 426, 452, 578, 711, 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.