Rmu_sc0002273.1_g000005

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002273.1
OG0000308
Physical Location & Seq
Forward (+)
30968 .. 31826
859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002273.1_g000005.1.cds

Sequence Viewer

Length: 681 bp
atggcaacaattgatgcaatggcagcccgcctagtctcctccttagctttggctgatggaagaaacctgtggatctgcgtccttccaaaggttggcacggcggatcaatggcagactgagtgtcgaagaaatgatgatagggtcttgttcacctttactgacccatcagatgtggacagagtgtggaaaggaggaccctggggatacaatagagctccggtagccgtagcgccatatgatggcatcactccggtagcagacgtgcccctgaagaaatcatcctactggatgacgctgcaaggagtaccgcctgcgttccgagtcgagattgattatgagaagcctctactctttcgtcggcgatttttggtcgaagacgaagtccagattttggtgcaattccggtatgacaaactctttggtcgttgtggaacttgtggactagtcactcatgtcggccttccctgcttagggccgaaactggttgaagatagtgacgcatcggtgggggtggtggcacaacccatggctctgacggtgctacgaggtcatgcaacaccgaaccctaatgctgataggatcgtgattaacaatcaaaccttgatttttaaggcccagataccaaggagcataccagaaaatctcctccttgcatcggtggtagccatgcgtcgtaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

226

Amino Acids

25.1

Weight (kDa)

8.63

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000308)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24891 FvH4_4g09031
rosa_multiflora Rmu_co8001542.1_g000001 Rmu_co8074240.1_g000001 Rmu_co8092076.1_g000001 Rmu_co8136936.1_g000001 Rmu_co8303129.1_g000001 Rmu_co8326579.1_g000001 Rmu_co8378475.1_g000001 Rmu_co8409011.1_g000001 Rmu_co8449663.1_g000001 Rmu_co8463085.1_g000001 Rmu_sc0000146.1_g000025 Rmu_sc0000294.1_g000018 Rmu_sc0000299.1_g000003 Rmu_sc0000342.1_g000003 Rmu_sc0000359.1_g000009 Rmu_sc0000431.1_g000022 Rmu_sc0000442.1_g000006 Rmu_sc0000458.1_g000011 Rmu_sc0000560.1_g000013 Rmu_sc0000594.1_g000075 Rmu_sc0000740.1_g000033 Rmu_sc0000839.1_g000002 Rmu_sc0000939.1_g000034 Rmu_sc0000955.1_g000002 Rmu_sc0001069.1_g000045 Rmu_sc0001074.1_g000019 Rmu_sc0001418.1_g000003 Rmu_sc0001484.1_g000006 Rmu_sc0001499.1_g000062 Rmu_sc0001631.1_g000002 Rmu_sc0001639.1_g000021 Rmu_sc0002146.1_g000007 Rmu_sc0002146.1_g000010 Rmu_sc0002260.1_g000065 Rmu_sc0002273.1_g000005 Rmu_sc0002310.1_g000016 Rmu_sc0002414.1_g000051 Rmu_sc0002479.1_g000003 Rmu_sc0002586.1_g000034 Rmu_sc0002586.1_g000035 Rmu_sc0002589.1_g000034 Rmu_sc0003187.1_g000020 Rmu_sc0003444.1_g000018 Rmu_sc0003633.1_g000041 Rmu_sc0003973.1_g000010 Rmu_sc0004209.1_g000004 Rmu_sc0004927.1_g000020 Rmu_sc0004950.1_g000007 Rmu_sc0004952.1_g000012 Rmu_sc0004966.1_g000001 Rmu_sc0006168.1_g000026 Rmu_sc0006325.1_g000003 Rmu_sc0006413.1_g000015 Rmu_sc0007192.1_g000002 Rmu_sc0007390.1_g000004 Rmu_sc0007847.1_g000003 Rmu_sc0008044.1_g000001 Rmu_sc0008101.1_g000008 Rmu_sc0008442.1_g000015 Rmu_sc0008714.1_g000004 Rmu_sc0009057.1_g000009 Rmu_sc0009916.1_g000015 Rmu_sc0010180.1_g000003 Rmu_sc0010560.1_g000005 Rmu_sc0010715.1_g000005 Rmu_sc0011022.1_g000002 Rmu_sc0011509.1_g000006 Rmu_sc0011831.1_g000006 Rmu_sc0012010.1_g000002 Rmu_sc0013320.1_g000007 Rmu_sc0013801.1_g000006 Rmu_sc0013801.1_g000007 Rmu_sc0013887.1_g000002 Rmu_sc0014485.1_g000014 Rmu_sc0018736.1_g000001 Rmu_sc0019355.1_g000001 Rmu_sc0020316.1_g000002 Rmu_sc0021214.1_g000001 Rmu_sc0021375.1_g000001 Rmu_sc0023412.1_g000001 Rmu_sc0024234.1_g000001 Rmu_sc0027415.1_g000001 Rmu_sc0029109.1_g000001 Rmu_sc0031708.1_g000001 Rmu_sc0035307.1_g000001 Rmu_sc0036546.1_g000001 Rmu_sc0037386.1_g000005 Rmu_sc0039204.1_g000001 Rmu_sc0039709.1_g000001 Rmu_sc0039867.1_g000001 Rmu_sc0040008.1_g000002 Rmu_sc0040009.1_g000002 Rmu_sc0041433.1_g000001 Rmu_sc0043076.1_g000001 Rmu_ssc0000024.1_g000022 Rmu_ssc0000065.1_g000003 Rmu_ssc0000100.1_g000036 Rmu_ssc0000171.1_g000015 Rmu_ssc0000190.1_g000018 Rmu_ssc0000226.1_g000017 Rmu_ssc0000244.1_g000039 Rmu_ssc0000419.1_g000008 Rmu_ssc0000451.1_g000014
rosa_roxburghii Rroxscaffold_1G00006150 Rroxscaffold_3G00219360 Rroxscaffold_5G00368320 Rroxscaffold_7G00191310
rosa_rugosa Rorug04G0060300 Rorug04G0115300 Rorug04G0128800
rosa_samantha Rh1AG029600 Rh2BG406100 Rh4DG128300 Rh5DG195500 Rh6AG427300 Rh6CG440900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 92, 239, 391
AciI CCGC 3 cut(s) 28, 101, 308
AclWI GGATC 3 cut(s) 80, 111, 587
AcuI CTGAAG 1 cut(s) 290
AfaI GTAC 1 cut(s) 306
AfiI CCNNNNNNNGG 7 cut(s) 88, 92, 239, 391, 470, 471, 655
AgsI TTSAA 1 cut(s) 488
AhdI GACNNNNNGTC 1 cut(s) 120
AhlI ACTAGT 1 cut(s) 442
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 2 cut(s) 47, 215
AluI AGCT 2 cut(s) 47, 215
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 3 cut(s) 80, 111, 587
AoxI GGCC 3 cut(s) 457, 473, 612
ApeKI GCWGC 2 cut(s) 23, 295
ArsI GACNNNNNNTTYG 4 cut(s) 366, 398, 401, 433
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 3 cut(s) 194, 473, 613
AsuHPI GGTGA 1 cut(s) 142
AvaII GGWCC 1 cut(s) 194
BaeGI GKGCMC 1 cut(s) 267
BanII GRGCYC 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 2 cut(s) 35, 282
BccI CCATC 3 cut(s) 50, 172, 233
BceAI ACGGC 2 cut(s) 114, 209
BciT130I CCWGG 1 cut(s) 199
BciVI GTATCC 1 cut(s) 197
BcoDI GTCTC 1 cut(s) 40
BcuI ACTAGT 1 cut(s) 442
BfaI CTAG 2 cut(s) 32, 443
BfoI RGCGCY 1 cut(s) 233
BfuI GTATCC 1 cut(s) 197
BisI GCNGC 2 cut(s) 24, 296
BlsI GCNGC 2 cut(s) 25, 297
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 1 cut(s) 194
BmeRI GACNNNNNGTC 1 cut(s) 120
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 3 cut(s) 194, 473, 613
BmiI GGNNCC 1 cut(s) 196
BmrFI CCNGG 1 cut(s) 199
BmsI GCATC 4 cut(s) 4, 252, 509, 662
BpiI GAAGAC 1 cut(s) 381
Bpu10I CCTNAGC 2 cut(s) 43, 469
BsaJI CCNNGG 4 cut(s) 197, 198, 525, 623
BsaWI WCCGGW 3 cut(s) 217, 250, 402
Bsc4I CCNNNNNNNGG 7 cut(s) 88, 92, 239, 391, 470, 471, 655
Bse1I ACTGG 2 cut(s) 290, 486
Bse3DI GCAATG 1 cut(s) 24
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 4 cut(s) 197, 198, 525, 623
BseGI GGATG 2 cut(s) 278, 294
BseLI CCNNNNNNNGG 7 cut(s) 88, 92, 239, 391, 470, 471, 655
BseMI GCAATG 1 cut(s) 24
BseMII CTCAG 1 cut(s) 108
BseNI ACTGG 2 cut(s) 290, 486
BseRI GAGGAG 2 cut(s) 28, 635
BseSI GKGCMC 1 cut(s) 267
BseXI GCAGC 2 cut(s) 35, 282
BshFI GGCC 3 cut(s) 459, 475, 614
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 3 cut(s) 218, 251, 403
BslI CCNNNNNNNGG 7 cut(s) 88, 92, 239, 391, 470, 471, 655
BsmAI GTCTC 1 cut(s) 40
BsnI GGCC 3 cut(s) 459, 475, 614
Bsp1286I GDGCHC 2 cut(s) 217, 267
Bsp143I GATC 3 cut(s) 72, 103, 579
Bsp19I CCATGG 1 cut(s) 525
BspACI CCGC 3 cut(s) 28, 101, 308
BspANI GGCC 3 cut(s) 459, 475, 614
BspCNI CTCAG 1 cut(s) 109
BspLI GGNNCC 1 cut(s) 196
BspPI GGATC 3 cut(s) 80, 111, 587
BsrDI GCAATG 1 cut(s) 24
BsrI ACTGG 2 cut(s) 290, 486
BssECI CCNNGG 4 cut(s) 197, 198, 525, 623
BssMI GATC 3 cut(s) 72, 103, 579
BssT1I CCWWGG 2 cut(s) 525, 623
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 1 cut(s) 538
BstC8I GCNNGC 2 cut(s) 28, 312
BstDEI CTNAG 3 cut(s) 43, 117, 469
BstDSI CCRYGG 1 cut(s) 525
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 2 cut(s) 278, 294
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 3 cut(s) 75, 106, 582
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 3 cut(s) 72, 103, 579
BstMWI GCNNNNNNNGC 3 cut(s) 23, 221, 465
BstNI CCWGG 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 197
BstSLI GKGCMC 1 cut(s) 267
BstV1I GCAGC 2 cut(s) 35, 282
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 1 cut(s) 72
BstYI RGATCY 1 cut(s) 72
BsuI GTATCC 1 cut(s) 197
BsuRI GGCC 3 cut(s) 459, 475, 614
BtgI CCRYGG 1 cut(s) 525
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 2 cut(s) 278, 294
Cac8I GCNNGC 2 cut(s) 28, 312
CfoI GCGC 1 cut(s) 232
Cfr13I GGNCC 3 cut(s) 194, 473, 613
CseI GACGC 4 cut(s) 67, 301, 506, 659
Csp6I GTAC 1 cut(s) 305
CviAII CATG 4 cut(s) 452, 526, 551, 667
CviQI GTAC 1 cut(s) 305
DdeI CTNAG 3 cut(s) 43, 117, 469
DpnI GATC 3 cut(s) 74, 105, 581
DpnII GATC 3 cut(s) 72, 103, 579
DriI GACNNNNNGTC 1 cut(s) 120
Eam1105I GACNNNNNGTC 1 cut(s) 120
EciI GGCGGA 1 cut(s) 116
Ecl136II GAGCTC 1 cut(s) 215
Eco130I CCWWGG 2 cut(s) 525, 623
Eco24I GRGCYC 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 215
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoO109I RGGNCCY 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 197
EcoT14I CCWWGG 2 cut(s) 525, 623
EcoT38I GRGCYC 1 cut(s) 217
ErhI CCWWGG 2 cut(s) 525, 623
FaeI CATG 4 cut(s) 455, 529, 554, 670
FaiI YATR 9 cut(s) 235, 237, 336, 408, 453, 527, 552, 632, 668
FatI CATG 4 cut(s) 451, 525, 550, 666
FauI CCCGC 1 cut(s) 35
FauNDI CATATG 1 cut(s) 235
Fnu4HI GCNGC 2 cut(s) 24, 296
FokI GGATG 2 cut(s) 265, 301
FriOI GRGCYC 1 cut(s) 217
Fsp4HI GCNGC 2 cut(s) 24, 296
FspBI CTAG 2 cut(s) 32, 443
GlaI GCGC 1 cut(s) 231
GluI GCNGC 2 cut(s) 24, 296
HaeII RGCGCY 1 cut(s) 233
HaeIII GGCC 3 cut(s) 459, 475, 614
HapII CCGG 3 cut(s) 218, 251, 403
HgaI GACGC 4 cut(s) 67, 301, 506, 659
HhaI GCGC 1 cut(s) 232
Hin1II CATG 4 cut(s) 455, 529, 554, 670
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HinfI GANTC 1 cut(s) 321
HpaII CCGG 3 cut(s) 218, 251, 403
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 3 cut(s) 150, 175, 440
Hpy188I TCNGA 3 cut(s) 169, 320, 534
Hpy188III TCNNGA 3 cut(s) 325, 385, 583
Hpy8I GTNNAC 3 cut(s) 150, 175, 440
Hpy99I CGWCG 2 cut(s) 360, 675
HpyAV CCTTC 2 cut(s) 92, 470
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 5 cut(s) 17, 298, 397, 554, 653
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 221, 465
HpyF3I CTNAG 3 cut(s) 43, 117, 469
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 4 cut(s) 455, 529, 554, 670
HspAI GCGC 1 cut(s) 230
Kzo9I GATC 3 cut(s) 72, 103, 579
LmnI GCTCC 2 cut(s) 220, 627
Lsp1109I GCAGC 2 cut(s) 35, 282
LweI GCATC 4 cut(s) 4, 252, 509, 662
MaeI CTAG 2 cut(s) 32, 443
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 445, 494, 674
MalI GATC 3 cut(s) 74, 105, 581
MboI GATC 3 cut(s) 72, 103, 579
MboII GAAGA 5 cut(s) 72, 138, 283, 386, 500
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 1 cut(s) 72
MhlI GDGCHC 2 cut(s) 217, 267
MluCI AATT 2 cut(s) 9, 398
MlyI GAGTC 1 cut(s) 330
MnlI CCTC 5 cut(s) 49, 185, 354, 539, 656
MseI TTAA 2 cut(s) 588, 609
MspI CCGG 3 cut(s) 218, 251, 403
MspR9I CCNGG 1 cut(s) 199
MunI CAATTG 1 cut(s) 9
MvaI CCWGG 1 cut(s) 199
MwoI GCNNNNNNNGC 3 cut(s) 23, 221, 465
NcoI CCATGG 1 cut(s) 525
NdeI CATATG 1 cut(s) 235
NdeII GATC 3 cut(s) 72, 103, 579
NlaIII CATG 4 cut(s) 455, 529, 554, 670
NlaIV GGNNCC 1 cut(s) 196
NmuCI GTSAC 2 cut(s) 445, 494
PasI CCCWGGG 1 cut(s) 198
PflFI GACNNNGTC 1 cut(s) 380
PflMI CCANNNNNTGG 3 cut(s) 92, 239, 391
PkrI GCNGC 2 cut(s) 25, 297
PleI GAGTC 1 cut(s) 329
PpsI GAGTC 1 cut(s) 329
PpuMI RGGWCCY 1 cut(s) 194
Psp124BI GAGCTC 1 cut(s) 217
Psp5II RGGWCCY 1 cut(s) 194
Psp6I CCWGG 1 cut(s) 197
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 1 cut(s) 196
PspPI GGNCC 3 cut(s) 194, 473, 613
PspPPI RGGWCCY 1 cut(s) 194
PsuI RGATCY 1 cut(s) 72
PsyI GACNNNGTC 1 cut(s) 380
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
SacI GAGCTC 1 cut(s) 217
SaqAI TTAA 2 cut(s) 588, 609
SatI GCNGC 2 cut(s) 24, 296
Sau3AI GATC 3 cut(s) 72, 103, 579
Sau96I GGNCC 3 cut(s) 194, 473, 613
SchI GAGTC 1 cut(s) 330
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 2 cut(s) 217, 267
SetI ASST 8 cut(s) 49, 69, 93, 155, 217, 264, 550, 602
SfaNI GCATC 4 cut(s) 4, 252, 509, 662
SinI GGWCC 1 cut(s) 194
SpeI ACTAGT 1 cut(s) 442
Sse9I AATT 2 cut(s) 9, 398
SsiI CCGC 3 cut(s) 28, 101, 308
SspMI CTAG 2 cut(s) 32, 443
SstI GAGCTC 1 cut(s) 217
StyD4I CCNGG 1 cut(s) 197
StyI CCWWGG 2 cut(s) 525, 623
TaaI ACNGT 1 cut(s) 538
TaiI ACGT 1 cut(s) 264
TaqI TCGA 3 cut(s) 124, 324, 372
TasI AATT 2 cut(s) 9, 398
Tru1I TTAA 2 cut(s) 588, 609
Tru9I TTAA 2 cut(s) 588, 609
TseFI GTSAC 2 cut(s) 445, 494
TseI GCWGC 2 cut(s) 23, 295
Tsp45I GTSAC 2 cut(s) 445, 494
Tth111I GACNNNGTC 1 cut(s) 380
Van91I CCANNNNNTGG 3 cut(s) 92, 239, 391
VpaK11BI GGWCC 1 cut(s) 194
XagI CCTNNNNNAGG 1 cut(s) 86
XspI CTAG 2 cut(s) 32, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.