Rmu_sc0019355.1_g000001

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0019355.1
OG0000308
Physical Location & Seq
Forward (+)
1 .. 1241
1241 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0019355.1_g000001.1.cds

Sequence Viewer

Length: 755 bp
ggagtactctcgtcgatctttcatggttggcaagttactcagtctgcatggaggactctcgtcgatctttcctgggaatgttccaggttgaagaagtcggagaagatgacagggtcttgttctcattcacggatccaagtaatgtggatagggtttggaaaggtggaccatggggatataacagagctccgatagcccttgcaccgtacgacggtgtcagctcgacagcgaaagtaccactgaagaagtcatcatactggataacgctgcaaggagtaccgccgacattcagattagagcgcatcatgccccttatcggaagcacagtaggcgagttcaaagagatcaatcgtccgacgtttaggaaccaggtcatgcgcattagagttgatattgactatgtgaaacccttgctatttcgtcgtcgtttctgggtggagaacaaaccgaaactgattgaaaacaatgatgcattggtaggggtggtggcgcaacctatggcgttgatagtgctatggggacaggcaaaaccaaaccctaatattaacagggtggtggtgaacaaccaaaccctagtcttcaaggcccagctgccatatggaacagtaggaaaaggaaagaatgcaaagaaagatggggaaccagataaggtaaagagcaagaagaccaacctgatgccgaggttgttggctactgataagcttgaggcaagtactagctctgaccctaaggggaaagagaaggtacttgcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

250

Amino Acids

28.11

Weight (kDa)

9.75

Isoelectric Point (pI)

31.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000308)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g24891 FvH4_4g09031
rosa_multiflora Rmu_co8001542.1_g000001 Rmu_co8074240.1_g000001 Rmu_co8092076.1_g000001 Rmu_co8136936.1_g000001 Rmu_co8303129.1_g000001 Rmu_co8326579.1_g000001 Rmu_co8378475.1_g000001 Rmu_co8409011.1_g000001 Rmu_co8449663.1_g000001 Rmu_co8463085.1_g000001 Rmu_sc0000146.1_g000025 Rmu_sc0000294.1_g000018 Rmu_sc0000299.1_g000003 Rmu_sc0000342.1_g000003 Rmu_sc0000359.1_g000009 Rmu_sc0000431.1_g000022 Rmu_sc0000442.1_g000006 Rmu_sc0000458.1_g000011 Rmu_sc0000560.1_g000013 Rmu_sc0000594.1_g000075 Rmu_sc0000740.1_g000033 Rmu_sc0000839.1_g000002 Rmu_sc0000939.1_g000034 Rmu_sc0000955.1_g000002 Rmu_sc0001069.1_g000045 Rmu_sc0001074.1_g000019 Rmu_sc0001418.1_g000003 Rmu_sc0001484.1_g000006 Rmu_sc0001499.1_g000062 Rmu_sc0001631.1_g000002 Rmu_sc0001639.1_g000021 Rmu_sc0002146.1_g000007 Rmu_sc0002146.1_g000010 Rmu_sc0002260.1_g000065 Rmu_sc0002273.1_g000005 Rmu_sc0002310.1_g000016 Rmu_sc0002414.1_g000051 Rmu_sc0002479.1_g000003 Rmu_sc0002586.1_g000034 Rmu_sc0002586.1_g000035 Rmu_sc0002589.1_g000034 Rmu_sc0003187.1_g000020 Rmu_sc0003444.1_g000018 Rmu_sc0003633.1_g000041 Rmu_sc0003973.1_g000010 Rmu_sc0004209.1_g000004 Rmu_sc0004927.1_g000020 Rmu_sc0004950.1_g000007 Rmu_sc0004952.1_g000012 Rmu_sc0004966.1_g000001 Rmu_sc0006168.1_g000026 Rmu_sc0006325.1_g000003 Rmu_sc0006413.1_g000015 Rmu_sc0007192.1_g000002 Rmu_sc0007390.1_g000004 Rmu_sc0007847.1_g000003 Rmu_sc0008044.1_g000001 Rmu_sc0008101.1_g000008 Rmu_sc0008442.1_g000015 Rmu_sc0008714.1_g000004 Rmu_sc0009057.1_g000009 Rmu_sc0009916.1_g000015 Rmu_sc0010180.1_g000003 Rmu_sc0010560.1_g000005 Rmu_sc0010715.1_g000005 Rmu_sc0011022.1_g000002 Rmu_sc0011509.1_g000006 Rmu_sc0011831.1_g000006 Rmu_sc0012010.1_g000002 Rmu_sc0013320.1_g000007 Rmu_sc0013801.1_g000006 Rmu_sc0013801.1_g000007 Rmu_sc0013887.1_g000002 Rmu_sc0014485.1_g000014 Rmu_sc0018736.1_g000001 Rmu_sc0019355.1_g000001 Rmu_sc0020316.1_g000002 Rmu_sc0021214.1_g000001 Rmu_sc0021375.1_g000001 Rmu_sc0023412.1_g000001 Rmu_sc0024234.1_g000001 Rmu_sc0027415.1_g000001 Rmu_sc0029109.1_g000001 Rmu_sc0031708.1_g000001 Rmu_sc0035307.1_g000001 Rmu_sc0036546.1_g000001 Rmu_sc0037386.1_g000005 Rmu_sc0039204.1_g000001 Rmu_sc0039709.1_g000001 Rmu_sc0039867.1_g000001 Rmu_sc0040008.1_g000002 Rmu_sc0040009.1_g000002 Rmu_sc0041433.1_g000001 Rmu_sc0043076.1_g000001 Rmu_ssc0000024.1_g000022 Rmu_ssc0000065.1_g000003 Rmu_ssc0000100.1_g000036 Rmu_ssc0000171.1_g000015 Rmu_ssc0000190.1_g000018 Rmu_ssc0000226.1_g000017 Rmu_ssc0000244.1_g000039 Rmu_ssc0000419.1_g000008 Rmu_ssc0000451.1_g000014
rosa_roxburghii Rroxscaffold_1G00006150 Rroxscaffold_3G00219360 Rroxscaffold_5G00368320 Rroxscaffold_7G00191310
rosa_rugosa Rorug04G0060300 Rorug04G0115300 Rorug04G0128800
rosa_samantha Rh1AG029600 Rh2BG406100 Rh4DG128300 Rh5DG195500 Rh6AG427300 Rh6CG440900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 379
AciI CCGC 1 cut(s) 280
AclWI GGATC 2 cut(s) 127, 140
AcuI CTGAAG 1 cut(s) 262
AfaI GTAC 6 cut(s) 6, 208, 236, 278, 714, 746
AfiI CCNNNNNNNGG 2 cut(s) 211, 316
AgsI TTSAA 4 cut(s) 91, 339, 460, 582
AjnI CCWGG 3 cut(s) 71, 83, 368
AluBI AGCT 5 cut(s) 187, 221, 591, 702, 719
AluI AGCT 5 cut(s) 187, 221, 591, 702, 719
Alw21I GWGCWC 1 cut(s) 189
AlwI GGATC 2 cut(s) 127, 140
AoxI GGCC 1 cut(s) 584
ApeKI GCWGC 2 cut(s) 267, 591
AspLEI GCGC 3 cut(s) 302, 380, 492
AspS9I GGNCC 2 cut(s) 166, 585
AsuHPI GGTGA 1 cut(s) 570
AvaII GGWCC 1 cut(s) 166
AxyI CCTNAGG 1 cut(s) 728
BamHI GGATCC 1 cut(s) 132
BanII GRGCYC 1 cut(s) 189
BarI GAAGNNNNNNTAC 2 cut(s) 238, 270
BbsI GAAGAC 2 cut(s) 570, 670
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 254, 578
BccI CCATC 1 cut(s) 628
BciT130I CCWGG 3 cut(s) 73, 85, 370
BfaI CTAG 2 cut(s) 574, 716
BisI GCNGC 2 cut(s) 268, 592
BlsI GCNGC 2 cut(s) 269, 593
BmcAI AGTACT 2 cut(s) 6, 714
Bme1390I CCNGG 3 cut(s) 73, 85, 370
Bme18I GGWCC 1 cut(s) 166
BmgT120I GGNCC 2 cut(s) 166, 585
BmiI GGNNCC 3 cut(s) 134, 367, 641
BmrFI CCNGG 3 cut(s) 73, 85, 370
BmsI GCATC 3 cut(s) 311, 459, 665
BoxI GACNNNNGTC 1 cut(s) 59
BpiI GAAGAC 2 cut(s) 570, 670
BpuEI CTTGAG 1 cut(s) 724
BsaJI CCNNGG 3 cut(s) 72, 169, 679
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 316
Bse1I ACTGG 1 cut(s) 262
Bse21I CCTNAGG 1 cut(s) 728
BseBI CCWGG 3 cut(s) 73, 85, 370
BseDI CCNNGG 3 cut(s) 72, 169, 679
BseLI CCNNNNNNNGG 2 cut(s) 211, 316
BseMII CTCAG 1 cut(s) 53
BseNI ACTGG 1 cut(s) 262
BseXI GCAGC 2 cut(s) 254, 578
BseYI CCCAGC 1 cut(s) 587
BshFI GGCC 1 cut(s) 586
BsiHKAI GWGCWC 1 cut(s) 189
BsiWI CGTACG 1 cut(s) 206
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 2 cut(s) 211, 316
BsmFI GGGAC 1 cut(s) 533
BsmI GAATGC 1 cut(s) 627
BsnI GGCC 1 cut(s) 586
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 4 cut(s) 15, 64, 132, 344
Bsp19I CCATGG 1 cut(s) 169
BspACI CCGC 1 cut(s) 280
BspANI GGCC 1 cut(s) 586
BspCNI CTCAG 1 cut(s) 52
BspLI GGNNCC 3 cut(s) 134, 367, 641
BspPI GGATC 2 cut(s) 127, 140
BsrI ACTGG 1 cut(s) 262
BssECI CCNNGG 3 cut(s) 72, 169, 679
BssMI GATC 4 cut(s) 15, 64, 132, 344
BssT1I CCWWGG 1 cut(s) 169
Bst2UI CCWGG 3 cut(s) 73, 85, 370
Bst4CI ACNGT 4 cut(s) 206, 214, 327, 606
BstDEI CTNAG 2 cut(s) 39, 728
BstDSI CCRYGG 1 cut(s) 169
BstHHI GCGC 3 cut(s) 302, 380, 492
BstKTI GATC 4 cut(s) 18, 67, 135, 347
BstMBI GATC 4 cut(s) 15, 64, 132, 344
BstMWI GCNNNNNNNGC 3 cut(s) 193, 306, 329
BstNI CCWGG 3 cut(s) 73, 85, 370
BstPAI GACNNNNGTC 1 cut(s) 59
BstSCI CCNGG 3 cut(s) 71, 83, 368
BstV1I GCAGC 2 cut(s) 254, 578
BstV2I GAAGAC 2 cut(s) 570, 670
BstX2I RGATCY 1 cut(s) 132
BstYI RGATCY 1 cut(s) 132
Bsu36I CCTNAGG 1 cut(s) 728
BsuRI GGCC 1 cut(s) 586
BtgI CCRYGG 1 cut(s) 169
BtsIMutI CAGTG 1 cut(s) 238
CfoI GCGC 3 cut(s) 302, 380, 492
Cfr13I GGNCC 2 cut(s) 166, 585
CsiI ACCWGGT 1 cut(s) 368
Csp6I GTAC 6 cut(s) 5, 207, 235, 277, 713, 745
CspCI CAANNNNNGTGG 2 cut(s) 125, 160
CviAII CATG 5 cut(s) 23, 48, 170, 306, 375
CviJI RGCY 8 cut(s) 187, 196, 221, 586, 591, 691, 702, 719
CviKI_1 RGCY 8 cut(s) 187, 196, 221, 586, 591, 691, 702, 719
CviQI GTAC 6 cut(s) 5, 207, 235, 277, 713, 745
DdeI CTNAG 2 cut(s) 39, 728
DpnI GATC 4 cut(s) 17, 66, 134, 346
DpnII GATC 4 cut(s) 15, 64, 132, 344
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 262
Eco81I CCTNAGG 1 cut(s) 728
EcoICRI GAGCTC 1 cut(s) 187
EcoRII CCWGG 3 cut(s) 71, 83, 368
EcoT14I CCWWGG 1 cut(s) 169
EcoT22I ATGCAT 1 cut(s) 474
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 5 cut(s) 26, 51, 173, 309, 378
FaqI GGGAC 1 cut(s) 533
FatI CATG 5 cut(s) 22, 47, 169, 305, 374
FauNDI CATATG 1 cut(s) 597
Fnu4HI GCNGC 2 cut(s) 268, 592
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 2 cut(s) 268, 592
FspAI RTGCGCAY 1 cut(s) 379
FspBI CTAG 2 cut(s) 574, 716
FspI TGCGCA 1 cut(s) 379
GlaI GCGC 3 cut(s) 301, 379, 491
GluI GCNGC 2 cut(s) 268, 592
GsaI CCCAGC 1 cut(s) 591
HaeIII GGCC 1 cut(s) 586
HhaI GCGC 3 cut(s) 302, 380, 492
Hin1II CATG 5 cut(s) 26, 51, 173, 309, 378
Hin6I GCGC 3 cut(s) 300, 378, 490
HinP1I GCGC 3 cut(s) 300, 378, 490
HindIII AAGCTT 1 cut(s) 700
HinfI GANTC 1 cut(s) 55
HphI GGTGA 1 cut(s) 570
Hpy166II GTNNAC 2 cut(s) 166, 561
Hpy188I TCNGA 6 cut(s) 100, 191, 292, 319, 356, 723
Hpy8I GTNNAC 2 cut(s) 166, 561
Hpy99I CGWCG 6 cut(s) 16, 65, 214, 360, 425, 428
HpyAV CCTTC 1 cut(s) 735
HpyCH4III ACNGT 4 cut(s) 206, 214, 327, 606
HpyCH4IV ACGT 1 cut(s) 358
HpyCH4V TGCA 5 cut(s) 47, 202, 270, 472, 625
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 306, 329
HpyF3I CTNAG 2 cut(s) 39, 728
HpySE526I ACGT 1 cut(s) 358
Hsp92II CATG 5 cut(s) 26, 51, 173, 309, 378
HspAI GCGC 3 cut(s) 300, 378, 490
Kzo9I GATC 4 cut(s) 15, 64, 132, 344
LmnI GCTCC 1 cut(s) 192
Lsp1109I GCAGC 2 cut(s) 254, 578
LweI GCATC 3 cut(s) 311, 459, 665
MabI ACCWGGT 1 cut(s) 368
MaeI CTAG 2 cut(s) 574, 716
MaeII ACGT 1 cut(s) 358
MaeIII GTNAC 1 cut(s) 34
MalI GATC 4 cut(s) 17, 66, 134, 346
MboI GATC 4 cut(s) 15, 64, 132, 344
MboII GAAGA 5 cut(s) 103, 115, 255, 570, 675
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 189
MlyI GAGTC 1 cut(s) 49
MmeI TCCRAC 2 cut(s) 78, 379
MnlI CCTC 3 cut(s) 45, 674, 699
Mph1103I ATGCAT 1 cut(s) 474
MseI TTAA 2 cut(s) 545, 753
MspA1I CMGCKG 1 cut(s) 591
MspR9I CCNGG 3 cut(s) 73, 85, 370
Mva1269I GAATGC 1 cut(s) 627
MvaI CCWGG 3 cut(s) 73, 85, 370
MwoI GCNNNNNNNGC 3 cut(s) 193, 306, 329
NcoI CCATGG 1 cut(s) 169
NdeI CATATG 1 cut(s) 597
NdeII GATC 4 cut(s) 15, 64, 132, 344
NlaIII CATG 5 cut(s) 26, 51, 173, 309, 378
NlaIV GGNNCC 3 cut(s) 134, 367, 641
NmeAIII GCCGAG 1 cut(s) 704
NsbI TGCGCA 1 cut(s) 379
NsiI ATGCAT 1 cut(s) 474
PctI GAATGC 1 cut(s) 627
Pfl23II CGTACG 1 cut(s) 206
PflFI GACNNNGTC 2 cut(s) 112, 214
PkrI GCNGC 2 cut(s) 269, 593
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
PshAI GACNNNNGTC 1 cut(s) 59
Psp124BI GAGCTC 1 cut(s) 189
Psp6I CCWGG 3 cut(s) 71, 83, 368
PspFI CCCAGC 1 cut(s) 587
PspGI CCWGG 3 cut(s) 71, 83, 368
PspLI CGTACG 1 cut(s) 206
PspN4I GGNNCC 3 cut(s) 134, 367, 641
PspPI GGNCC 2 cut(s) 166, 585
PsuI RGATCY 1 cut(s) 132
PsyI GACNNNGTC 2 cut(s) 112, 214
PvuII CAGCTG 1 cut(s) 591
RsaI GTAC 6 cut(s) 6, 208, 236, 278, 714, 746
RsaNI GTAC 6 cut(s) 5, 207, 235, 277, 713, 745
SacI GAGCTC 1 cut(s) 189
SaqAI TTAA 2 cut(s) 545, 753
SatI GCNGC 2 cut(s) 268, 592
Sau3AI GATC 4 cut(s) 15, 64, 132, 344
Sau96I GGNCC 2 cut(s) 166, 585
ScaI AGTACT 2 cut(s) 6, 714
SchI GAGTC 1 cut(s) 49
ScrFI CCNGG 3 cut(s) 73, 85, 370
SduI GDGCHC 1 cut(s) 189
SexAI ACCWGGT 1 cut(s) 368
SfaNI GCATC 3 cut(s) 311, 459, 665
SinI GGWCC 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 703
SmoI CTYRAG 1 cut(s) 703
SsiI CCGC 1 cut(s) 280
SspI AATATT 1 cut(s) 543
SspMI CTAG 2 cut(s) 574, 716
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 3 cut(s) 71, 83, 368
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 4 cut(s) 206, 214, 327, 606
TaiI ACGT 1 cut(s) 361
TaqI TCGA 3 cut(s) 14, 63, 223
TatI WGTACW 2 cut(s) 4, 712
Tru1I TTAA 2 cut(s) 545, 753
Tru9I TTAA 2 cut(s) 545, 753
TscAI CASTG 1 cut(s) 245
TseI GCWGC 2 cut(s) 267, 591
TspDTI ATGAA 1 cut(s) 11
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 1 cut(s) 245
Tth111I GACNNNGTC 2 cut(s) 112, 214
VpaK11BI GGWCC 1 cut(s) 166
XcmI CCANNNNNNNNNTGG 1 cut(s) 595
XspI CTAG 2 cut(s) 574, 716
ZrmI AGTACT 2 cut(s) 6, 714
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.