Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0005414

Physical Location & Seq

Genomic Coordinates Forward (+)

21792248 .. 21798627

Gene Structure 6380 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2808 bp

ATGCAAGATCGATCAGATGGGAAACAGTGGAGGTGCACTGCCATGTCTATGCCAGATAAAACGGTATGTGAAAAACATTACATCCAGGCAAAGAAGAGGGCAGCTAATTCTGCAATGCGAGCAAATTTGAAAAAGGCCAAGAGGAAGTCGTTAGGTGAAAGTGATATTTACTTGGAGAGTAAGAGTGATGATTTGGATGTACCACTTGCTAGTGTGAAGTCACAGGAGAGAAAGTACATGGAGAAGGTATCAAAAAATCATTTTCGTTATTCACTTGAGAGACCACCTGTGAAGGGTTTATCCGTGCGTAATTCTCCAAAACCAAAGGACGAGATGGACTTGGAGGAGTATGAGGAAAATTGGAGGTCTAATAAGTCACCACCTGCTAATGCTTTGGACTCATCCAGGAACAGACCGCAGAGGAGTTTTGATGTTAATGCTATGACAGTTTCGGAAGACTCTGATGGAAGATCAGAATCCTCTGAAGAAACTGGTGGCCAAACTTGCCATCAGTGTCGGAGGAATGACAGAGAGACAGTCATTTGGTGCCTCAAATGTGATAGAAGAGGATACTGTGACAGCTGTATTTCAACATGGTACTCGGACATTCCATTAGAAGACATTCAGAGGTCCTGTCCGGCATGTCGTGGCACTTGTAATTGCAGAGTGTGCCTACGCAGAGATAATTTGGTAAAGGTAAGGATAAGAGAGATACCAGTGCTAGATAAGTTGAAATATCTCCACTGTCTGCTGTCTTCAGTGCTTCCTATAGTTAAGCAAATCCATCAAGTACAATGCTTTGAAGTTGAACTGGAAAAAAAGCTTCGCGGAACCGATATAGATCTTGCCCGGACAAAATTGAATGCAGATGAGCAGATGTGCTGCAATTTCTGTAGAATTCCCATCATTGATTATCATTGGCACTGTTCCCGCTGCGCGTATGATCTCTGCCTTAGCCCTTGTCGAGATCTTCGAGAAGCATCCATGCCTGGTGTTAAAGGAGAGGTGGTAGAGAATCAGATTGGTGAGGATAGGCGAGAAAAAGAAACCAAGTTGGAGCAACCAAAATCGTCCAAAGTCAGTGTAAATTTAGCAGACAAGTTTTCCAACTGGAAAGCCCACAGTGATGGAAGTATCCCTTGCCCTCCAAAGGAGTATGGTGGCTGTGGCCATTCATCATTGAATTTAAGCCGTATCTTTAAGATGAACTGGGTTGCAAAACTAGTAAAAAATGCAGAGGAAATGGTTAGTGGCTGTAGAGTTAATGATGCTGTCAATCTAGAGAAAACTGGGCTTAACGATCCAAGACTTAGCCAGTATGCTCATAGAGAGGATAGCGATAATTTCTTGTACTGTGCTGCATCTGAAGATATCAAATCTGATGGGATTGGCAGTTTTAAAATGCATTGGCATAGGGGTGAGCCCATCATTGTTAAGGAGGTGTTTGATAGCTCATCAATTTCAAGCTGGGATCCAATGGTTATATGGAGAGGGATTCGGGAGACCACAGATGAGAAATTAAAGGATGAGAACAGAAGGGTGAAGGCCATAAATTGCTGTGACTGGTCTGAGGTTGATATTGAACTTAGTGAATTCATGAAAGGCTACTCCGAGGGAAGAGTTAATGAAAATGGTATGCCAGAAATGTTGAAACTCAAAGACTGGCCTTCTCCTAGTGCATCTGAAGAGTTTTTATTATATCAGAGACCTGAATTTATCAGCAGACTGCCTTTACTTGAGTATATTCACTCCAAGTTTGGTCTTTTAAATGTTACTGCAAAACTGCCACACTATTCTTTGCAAAACGAGGTTGGACCTAAGATTTTTATTTCTTATGGGACCTATGAGGAACTTGGTAGACACGACTCCTTGACCAATCTCCATTTCAACATGCGTGACATGGTATACCTGCTGGTGCACGCATGTGAGGTAAAGCTAAAAGGTTTGCAGAAGACAAAGATTGAGAACACACAAAAATCCTTCGAGGAATCTGAGGTTGAAGAATCATTCGGGGATCTTCAAATGGGTATGGGAGAGGATACAAGGTCTGATGTATCACTTCTTGGTCAGAATGTGGAGAATGAATATGGGGCAACTTTGGCCTCAGATAAAGATGAGAGTACGGCAGAGCATGGACATGAATCCACTCCTATGGTTGAAGATGACACCGCCAACTGTGAACAGTCAGAGAGAGACGGTAAAGATGTCTCTGAAAAAATTCATATGGGAGTTCTTTGGGATGTTTTCCGTCGACAGGATGTTCCAAAGCTGACTGAGTATTTGAGAATACATTGGCAAGAATTTGGGAAGCTCAATGAAACAAATATTTTTGTAACATCACCTCTTTATGATGGGACATTGTTCCTGAATGGGGATCATAAAAGAAAATTGAAGGAGGAATTTGGAATTGAGCCATGGTCCTTTGAACAACATTTGGGGCAAGCTGTTTTTATCCCCGCTGGATGCCCTTTTCAAGTCAGAAGTCTTCAGTCAACTGTTCAGCTGGGTCTTGATTTCTTATCTCCCGAAAGCCTCAGTGAGGCTGCACGACTAGCTGATGAGATTCGCTGTCTTCCAAATGACCATGAAGCAAAGCTACAAGTGTTGGAGGTTGGACAACGGAAATTTTCCTTAGAAGTAGGAAAGATATCACTTTATGCGGCAAGTTCAGCCATTAAAGAGATTCAGAAGTTGGTACTCGATCCAAAACTTGGAGCAGAACTTGGATTTGAAGACCCAAATTTGACCGCGGCAGTTTCTGAGAATTTGGAGAAAATGACTAAACGAAGACAGATAACTTGTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008214 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031326 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0033169 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03010 ko04714 map03010 map04714

Pfam Domains

Protein Families

JmjC WRC zf-4CXXC_R1

Protein Analysis

936

Amino Acids

106.78

Weight (kDa)

5.81

Isoelectric Point (pI)

49.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRC	PF08879	4 - 37	9.2e-10	WRC
zf-4CXXC_R1	PF10497	164 - 231	2.8e-08	Zinc-finger domain of monoamine-oxidase A repressor R1
JmjC	PF02373	741 - 835	1.3e-07	JmjC domain, hydroxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005414)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09060 AT1G09060 AT1G09060 AT1G09060
fragaria_vesca	FvH4_6g06290 FvH4_6g06290 FvH4_6g06290 FvH4_6g06290
malus_domestica	MD04G1202800.v1.1 MD12G1216600.v1.1
prunus_persica	Prupe.6G322900_v2.0.a1 Prupe.6G322900_v2.0.a1 Prupe.6G322900_v2.0.a1 Prupe.6G322900_v2.0.a1 Prupe.6G322900_v2.0.a1
pyrus_communis	pycom04g17890 pycom12g20210
rosa_chinensis	RchiOBHm_Chr3g0455331
rosa_laevigata	RLG00000025369
rosa_multiflora	Rmu_sc0002169.1_g000043
rosa_roxburghii	Rroxscaffold_6G00424370
rosa_rugosa	Rorug03G0006300 Rorug03G0006400 Rorug03G0006500
rosa_samantha	Rh3AG066100 Rh3BG068300 Rh3CG066700 Rh3DG068200
rosa_wichuraiana	Rw3G005210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	391
Acc36I	ACCTGC	2 cut(s)	391, 1917
AccB1I	GGYRCC	1 cut(s)	546
AccB7I	CCANNNNNTGG	1 cut(s)	2711
AccI	GTMKAC	3 cut(s)	1858, 1905, 2251
AccII	CGCG	3 cut(s)	828, 938, 2750
AciI	CCGC	8 cut(s)	416, 828, 931, 2169, 2457, 2660, 2748, 2750
AclWI	GGATC	6 cut(s)	1295, 1466, 1479, 2022, 2382, 2696
AcoI	YGGCCR	2 cut(s)	496, 1168
AcuI	CTGAAG	5 cut(s)	504, 741, 1386, 1704, 2471
AfaI	GTAC	7 cut(s)	201, 236, 599, 792, 1352, 2122, 2697
AfiI	CCNNNNNNNGG	6 cut(s)	293, 1150, 2254, 2371, 2539, 2711
AhlI	ACTAGT	1 cut(s)	1222
AjnI	CCWGG	3 cut(s)	84, 404, 988
AjuI	GAANNNNNNNTTGG	2 cut(s)	1868, 1900
AleI	CACNNNNGTG	1 cut(s)	1923
Alw21I	GWGCWC	2 cut(s)	38, 1920
Alw26I	GTCTC	6 cut(s)	274, 527, 1496, 1699, 2187, 2212
Alw44I	GTGCAC	2 cut(s)	34, 1916
AlwI	GGATC	6 cut(s)	1295, 1466, 1479, 2022, 2382, 2696
AlwNI	CAGNNNCTG	1 cut(s)	2759
AoxI	GGCC	6 cut(s)	135, 496, 1168, 1545, 1664, 2100
ApaLI	GTGCAC	2 cut(s)	34, 1916
ApeKI	GCWGC	5 cut(s)	101, 882, 933, 1358, 2543
ArsI	GACNNNNNNTTYG	2 cut(s)	2263, 2295
Asp700I	GAANNNNTTC	2 cut(s)	621, 2217
AspLEI	GCGC	1 cut(s)	938
AspS9I	GGNCC	4 cut(s)	630, 1814, 1839, 2418
AsuC2I	CCSGG	1 cut(s)	850
AsuHPI	GGTGA	6 cut(s)	167, 369, 1037, 1430, 1552, 2331
AvaII	GGWCC	4 cut(s)	630, 1814, 1839, 2418
BaeGI	GKGCMC	2 cut(s)	38, 1920
BalI	TGGCCA	2 cut(s)	498, 1170
BamHI	GGATCC	1 cut(s)	1471
BanI	GGYRCC	1 cut(s)	546
BanII	GRGCYC	1 cut(s)	1425
BarI	GAAGNNNNNNTAC	2 cut(s)	2580, 2612
BbsI	GAAGAC	8 cut(s)	462, 624, 747, 1958, 2477, 2564, 2739, 2794
Bbv12I	GWGCWC	2 cut(s)	38, 1920
BbvI	GCAGC	5 cut(s)	113, 869, 920, 1345, 2530
BceAI	ACGGC	2 cut(s)	1176, 2139
BciT130I	CCWGG	3 cut(s)	86, 406, 990
BciVI	GTATCC	3 cut(s)	563, 1145, 2032
BcnI	CCSGG	1 cut(s)	850
BcoDI	GTCTC	6 cut(s)	274, 527, 1496, 1699, 2187, 2212
BcuI	ACTAGT	1 cut(s)	1222
BfaI	CTAG	6 cut(s)	210, 722, 1223, 1280, 1674, 2552
BfmI	CTRYAG	3 cut(s)	768, 892, 1255
BfuAI	ACCTGC	2 cut(s)	391, 1917
BfuI	GTATCC	3 cut(s)	563, 1145, 2032
BglII	AGATCT	2 cut(s)	841, 967
BisI	GCNGC	7 cut(s)	102, 883, 934, 1359, 2544, 2661, 2751
BlsI	GCNGC	7 cut(s)	103, 884, 935, 1360, 2545, 2662, 2752
Bme1390I	CCNGG	4 cut(s)	86, 406, 850, 990
Bme18I	GGWCC	4 cut(s)	630, 1814, 1839, 2418
BmgT120I	GGNCC	4 cut(s)	630, 1814, 1839, 2418
BmiI	GGNNCC	4 cut(s)	548, 832, 1473, 1840
BmrFI	CCNGG	4 cut(s)	86, 406, 850, 990
BmrI	ACTGGG	2 cut(s)	1219, 1299
BmsI	GCATC	5 cut(s)	989, 1258, 1370, 1688, 2453
BmuI	ACTGGG	2 cut(s)	1219, 1299
BpiI	GAAGAC	8 cut(s)	462, 624, 747, 1958, 2477, 2564, 2739, 2794
Bpu10I	CCTNAGC	1 cut(s)	953
BpuEI	CTTGAG	2 cut(s)	296, 1757
BpuMI	CCSGG	1 cut(s)	850
Bsa29I	ATCGAT	1 cut(s)	10
BsaBI	GATNNNNATC	2 cut(s)	475, 840
BsaI	GGTCTC	3 cut(s)	274, 1496, 1699
BsaJI	CCNNGG	3 cut(s)	1611, 2414, 2748
BsaXI	ACNNNNNCTCC	4 cut(s)	218, 248, 993, 1023
Bsc4I	CCNNNNNNNGG	6 cut(s)	293, 1150, 2254, 2371, 2539, 2711
Bse1I	ACTGG	9 cut(s)	496, 716, 816, 1115, 1214, 1294, 1315, 1568, 1667
Bse3DI	GCAATG	1 cut(s)	120
Bse8I	GATNNNNATC	2 cut(s)	475, 840
BseBI	CCWGG	3 cut(s)	86, 406, 990
BseCI	ATCGAT	1 cut(s)	10
BseDI	CCNNGG	3 cut(s)	1611, 2414, 2748
BseGI	GGATG	8 cut(s)	81, 202, 401, 980, 1531, 2245, 2263, 2468
BseJI	GATNNNNATC	2 cut(s)	475, 840
BseLI	CCNNNNNNNGG	6 cut(s)	293, 1150, 2254, 2371, 2539, 2711
BseMI	GCAATG	1 cut(s)	120
BseMII	CTCAG	6 cut(s)	1560, 1983, 2118, 2265, 2548, 2751
BseNI	ACTGG	9 cut(s)	496, 716, 816, 1115, 1214, 1294, 1315, 1568, 1667
BseRI	GAGGAG	2 cut(s)	359, 436
BseSI	GKGCMC	2 cut(s)	38, 1920
BseXI	GCAGC	5 cut(s)	113, 869, 920, 1345, 2530
BseYI	CCCAGC	2 cut(s)	1467, 2503
BsgI	GTGCAG	1 cut(s)	2529
Bsh1236I	CGCG	3 cut(s)	828, 938, 2750
BshFI	GGCC	6 cut(s)	137, 498, 1170, 1547, 1666, 2102
BshNI	GGYRCC	1 cut(s)	546
BshVI	ATCGAT	1 cut(s)	10
BsiHKAI	GWGCWC	2 cut(s)	38, 1920
BsiSI	CCGG	2 cut(s)	638, 850
BslFI	GGGAC	2 cut(s)	1852, 2368
BslI	CCNNNNNNNGG	6 cut(s)	293, 1150, 2254, 2371, 2539, 2711
BsmAI	GTCTC	6 cut(s)	274, 527, 1496, 1699, 2187, 2212
BsmBI	CGTCTC	1 cut(s)	2187
BsmFI	GGGAC	2 cut(s)	1852, 2368
BsmI	GAATGC	1 cut(s)	868
BsnI	GGCC	6 cut(s)	137, 498, 1170, 1547, 1666, 2102
Bso31I	GGTCTC	3 cut(s)	274, 1496, 1699
Bsp1286I	GDGCHC	3 cut(s)	38, 1425, 1920
Bsp19I	CCATGG	1 cut(s)	2414
BspACI	CCGC	8 cut(s)	416, 828, 931, 2169, 2457, 2660, 2748, 2750
BspANI	GGCC	6 cut(s)	137, 498, 1170, 1547, 1666, 2102
BspCNI	CTCAG	6 cut(s)	1561, 1984, 2117, 2266, 2547, 2752
BspDI	ATCGAT	1 cut(s)	10
BspFNI	CGCG	3 cut(s)	828, 938, 2750
BspHI	TCATGA	1 cut(s)	1596
BspLI	GGNNCC	4 cut(s)	548, 832, 1473, 1840
BspMI	ACCTGC	2 cut(s)	391, 1917
BspPI	GGATC	6 cut(s)	1295, 1466, 1479, 2022, 2382, 2696
BspT107I	GGYRCC	1 cut(s)	546
BspTNI	GGTCTC	3 cut(s)	274, 1496, 1699
BsrDI	GCAATG	1 cut(s)	120
BsrI	ACTGG	9 cut(s)	496, 716, 816, 1115, 1214, 1294, 1315, 1568, 1667
BssECI	CCNNGG	3 cut(s)	1611, 2414, 2748
BssNAI	GTATAC	1 cut(s)	1906
BssT1I	CCWWGG	1 cut(s)	2414
Bst1107I	GTATAC	1 cut(s)	1906
Bst2UI	CCWGG	3 cut(s)	86, 406, 990
Bst6I	CTCTTC	4 cut(s)	89, 559, 1612, 1680
BstAPI	GCANNNNNTGC	1 cut(s)	669
BstC8I	GCNNGC	3 cut(s)	120, 1920, 2442
BstDSI	CCRYGG	2 cut(s)	2414, 2748
BstENI	CCTNNNNNAGG	1 cut(s)	2537
BstF5I	GGATG	8 cut(s)	81, 202, 401, 980, 1531, 2245, 2263, 2468
BstFNI	CGCG	3 cut(s)	828, 938, 2750
BstHHI	GCGC	1 cut(s)	938
BstMAI	GTCTC	6 cut(s)	274, 527, 1496, 1699, 2187, 2212
BstMWI	GCNNNNNNNGC	7 cut(s)	110, 119, 504, 669, 2099, 2552, 2669
BstNI	CCWGG	3 cut(s)	86, 406, 990
BstNSI	RCATGY	3 cut(s)	645, 1894, 1926
BstSCI	CCNGG	4 cut(s)	84, 404, 848, 988
BstSFI	CTRYAG	3 cut(s)	768, 892, 1255
BstSLI	GKGCMC	2 cut(s)	38, 1920
BstUI	CGCG	3 cut(s)	828, 938, 2750
BstV1I	GCAGC	5 cut(s)	113, 869, 920, 1345, 2530
BstV2I	GAAGAC	8 cut(s)	462, 624, 747, 1958, 2477, 2564, 2739, 2794
BstX2I	RGATCY	4 cut(s)	841, 967, 1471, 2014
BstXI	CCANNNNNNTGG	2 cut(s)	1127, 2152
BstYI	RGATCY	4 cut(s)	841, 967, 1471, 2014
BstZ17I	GTATAC	1 cut(s)	1906
Bsu15I	ATCGAT	1 cut(s)	10
BsuI	GTATCC	3 cut(s)	563, 1145, 2032
BsuRI	GGCC	6 cut(s)	137, 498, 1170, 1547, 1666, 2102
BsuTUI	ATCGAT	1 cut(s)	10
BtgI	CCRYGG	2 cut(s)	2414, 2748
BtsCI	GGATG	8 cut(s)	81, 202, 401, 980, 1531, 2245, 2263, 2468
BtsI	GCAGTG	1 cut(s)	36
BveI	ACCTGC	2 cut(s)	391, 1917
Cac8I	GCNNGC	3 cut(s)	120, 1920, 2442
CaiI	CAGNNNCTG	1 cut(s)	2759
CciI	TCATGA	1 cut(s)	1596
CfoI	GCGC	1 cut(s)	938
Cfr13I	GGNCC	4 cut(s)	630, 1814, 1839, 2418
Cfr42I	CCGCGG	1 cut(s)	2751
ClaI	ATCGAT	1 cut(s)	10
Csp6I	GTAC	7 cut(s)	200, 235, 598, 791, 1351, 2121, 2696
CviQI	GTAC	7 cut(s)	200, 235, 598, 791, 1351, 2121, 2696
DraI	TTTAAA	2 cut(s)	1399, 1767
EaeI	YGGCCR	2 cut(s)	496, 1168
Eam1104I	CTCTTC	4 cut(s)	89, 559, 1612, 1680
EarI	CTCTTC	4 cut(s)	89, 559, 1612, 1680
Eco130I	CCWWGG	1 cut(s)	2414
Eco24I	GRGCYC	1 cut(s)	1425
Eco31I	GGTCTC	3 cut(s)	274, 1496, 1699
Eco32I	GATATC	2 cut(s)	1372, 2649
Eco47I	GGWCC	4 cut(s)	630, 1814, 1839, 2418
Eco57I	CTGAAG	5 cut(s)	504, 741, 1386, 1704, 2471
EcoNI	CCTNNNNNAGG	1 cut(s)	2537
EcoO109I	RGGNCCY	2 cut(s)	630, 1839
EcoRI	GAATTC	2 cut(s)	897, 1592
EcoRII	CCWGG	3 cut(s)	84, 404, 988
EcoRV	GATATC	2 cut(s)	1372, 2649
EcoT14I	CCWWGG	1 cut(s)	2414
EcoT22I	ATGCAT	1 cut(s)	1407
EcoT38I	GRGCYC	1 cut(s)	1425
ErhI	CCWWGG	1 cut(s)	2414
Esp3I	CGTCTC	1 cut(s)	2187
FalI	AAGNNNNNCTT	2 cut(s)	1123, 1155
FaqI	GGGAC	2 cut(s)	1852, 2368
FauI	CCCGC	2 cut(s)	938, 2464
FauNDI	CATATG	1 cut(s)	2223
FblI	GTMKAC	3 cut(s)	1858, 1905, 2251
Fnu4HI	GCNGC	7 cut(s)	102, 883, 934, 1359, 2544, 2661, 2751
FokI	GGATG	8 cut(s)	68, 209, 388, 967, 1538, 2252, 2270, 2475
FriOI	GRGCYC	1 cut(s)	1425
Fsp4HI	GCNGC	7 cut(s)	102, 883, 934, 1359, 2544, 2661, 2751
FspBI	CTAG	6 cut(s)	210, 722, 1223, 1280, 1674, 2552
GlaI	GCGC	1 cut(s)	937
GluI	GCNGC	7 cut(s)	102, 883, 934, 1359, 2544, 2661, 2751
GsaI	CCCAGC	2 cut(s)	1471, 2507
HaeIII	GGCC	6 cut(s)	137, 498, 1170, 1547, 1666, 2102
HapII	CCGG	2 cut(s)	638, 850
HhaI	GCGC	1 cut(s)	938
Hin6I	GCGC	1 cut(s)	936
HinP1I	GCGC	1 cut(s)	936
HincII	GTYRAC	2 cut(s)	2252, 2493
HindII	GTYRAC	2 cut(s)	2252, 2493
HindIII	AAGCTT	1 cut(s)	821
HpaII	CCGG	2 cut(s)	638, 850
HphI	GGTGA	6 cut(s)	167, 369, 1037, 1430, 1552, 2331
Hpy166II	GTNNAC	7 cut(s)	36, 1859, 1906, 1918, 2180, 2252, 2493
Hpy188III	TCNNGA	8 cut(s)	965, 974, 1280, 1499, 1597, 2365, 2510, 2525
Hpy8I	GTNNAC	7 cut(s)	36, 1859, 1906, 1918, 2180, 2252, 2493
Hpy99I	CGWCG	1 cut(s)	2253
HpyAV	CCTTC	7 cut(s)	238, 286, 1530, 1537, 1677, 1990, 2386
HpyF10VI	GCNNNNNNNGC	7 cut(s)	110, 119, 504, 669, 2099, 2552, 2669
HspAI	GCGC	1 cut(s)	936
KspI	CCGCGG	1 cut(s)	2751
LmnI	GCTCC	2 cut(s)	1057, 2714
Lsp1109I	GCAGC	5 cut(s)	113, 869, 920, 1345, 2530
LweI	GCATC	5 cut(s)	989, 1258, 1370, 1688, 2453
MaeI	CTAG	6 cut(s)	210, 722, 1223, 1280, 1674, 2552
MaeIII	GTNAC	7 cut(s)	219, 375, 575, 1559, 1771, 1895, 2332
MflI	RGATCY	4 cut(s)	841, 967, 1471, 2014
MhlI	GDGCHC	3 cut(s)	38, 1425, 1920
MlsI	TGGCCA	2 cut(s)	498, 1170
MluNI	TGGCCA	2 cut(s)	498, 1170
MlyI	GAGTC	3 cut(s)	392, 452, 1859
MmeI	TCCRAC	6 cut(s)	497, 1035, 1131, 1792, 2586, 2593
Mox20I	TGGCCA	2 cut(s)	498, 1170
Mph1103I	ATGCAT	1 cut(s)	1407
MroXI	GAANNNNTTC	2 cut(s)	621, 2217
MscI	TGGCCA	2 cut(s)	498, 1170
MslI	CAYNNNNRTG	7 cut(s)	41, 47, 1125, 1416, 1923, 2136, 2150
Msp20I	TGGCCA	2 cut(s)	498, 1170
MspA1I	CMGCKG	5 cut(s)	582, 933, 2459, 2503, 2750
MspI	CCGG	2 cut(s)	638, 850
MspR9I	CCNGG	4 cut(s)	86, 406, 850, 990
Mva1269I	GAATGC	1 cut(s)	868
MvaI	CCWGG	3 cut(s)	86, 406, 990
MvnI	CGCG	3 cut(s)	828, 938, 2750
MwoI	GCNNNNNNNGC	7 cut(s)	110, 119, 504, 669, 2099, 2552, 2669
NciI	CCSGG	1 cut(s)	850
NcoI	CCATGG	1 cut(s)	2414
NdeI	CATATG	1 cut(s)	2223
NlaIV	GGNNCC	4 cut(s)	548, 832, 1473, 1840
NmuCI	GTSAC	5 cut(s)	219, 375, 575, 1559, 1895
NsiI	ATGCAT	1 cut(s)	1407
NspI	RCATGY	3 cut(s)	645, 1894, 1926
OliI	CACNNNNGTG	1 cut(s)	1923
PagI	TCATGA	1 cut(s)	1596
PaqCI	CACCTGC	1 cut(s)	391
PcsI	WCGNNNNNNNCGW	1 cut(s)	970
PctI	GAATGC	1 cut(s)	868
PdmI	GAANNNNTTC	2 cut(s)	621, 2217
PfeI	GAWTC	8 cut(s)	476, 1015, 1495, 1988, 2003, 2141, 2563, 2683
PflMI	CCANNNNNTGG	1 cut(s)	2711
PfoI	TCCNGGA	1 cut(s)	404
PkrI	GCNGC	7 cut(s)	103, 884, 935, 1360, 2545, 2662, 2752
PleI	GAGTC	3 cut(s)	392, 452, 1859
PpsI	GAGTC	3 cut(s)	392, 452, 1859
PpuMI	RGGWCCY	2 cut(s)	630, 1839
Psp5II	RGGWCCY	2 cut(s)	630, 1839
Psp6I	CCWGG	3 cut(s)	84, 404, 988
PspFI	CCCAGC	2 cut(s)	1467, 2503
PspGI	CCWGG	3 cut(s)	84, 404, 988
PspN4I	GGNNCC	4 cut(s)	548, 832, 1473, 1840
PspPI	GGNCC	4 cut(s)	630, 1814, 1839, 2418
PspPPI	RGGWCCY	2 cut(s)	630, 1839
PstNI	CAGNNNCTG	1 cut(s)	2759
PsuI	RGATCY	4 cut(s)	841, 967, 1471, 2014
PvuII	CAGCTG	2 cut(s)	582, 2503
RsaI	GTAC	7 cut(s)	201, 236, 599, 792, 1352, 2122, 2697
RsaNI	GTAC	7 cut(s)	200, 235, 598, 791, 1351, 2121, 2696
RseI	CAYNNNNRTG	7 cut(s)	41, 47, 1125, 1416, 1923, 2136, 2150
SacII	CCGCGG	1 cut(s)	2751
SalI	GTCGAC	1 cut(s)	2250
SatI	GCNGC	7 cut(s)	102, 883, 934, 1359, 2544, 2661, 2751
Sau96I	GGNCC	4 cut(s)	630, 1814, 1839, 2418
SchI	GAGTC	3 cut(s)	392, 452, 1859
ScrFI	CCNGG	4 cut(s)	86, 406, 850, 990
SduI	GDGCHC	3 cut(s)	38, 1425, 1920
SfaNI	GCATC	5 cut(s)	989, 1258, 1370, 1688, 2453
SfcI	CTRYAG	3 cut(s)	768, 892, 1255
Sfr303I	CCGCGG	1 cut(s)	2751
SgrBI	CCGCGG	1 cut(s)	2751
SinI	GGWCC	4 cut(s)	630, 1814, 1839, 2418
SmiMI	CAYNNNNRTG	7 cut(s)	41, 47, 1125, 1416, 1923, 2136, 2150
SmlI	CTYRAG	2 cut(s)	275, 1736
SmoI	CTYRAG	2 cut(s)	275, 1736
SpeI	ACTAGT	1 cut(s)	1222
SsiI	CCGC	8 cut(s)	416, 828, 931, 2169, 2457, 2660, 2748, 2750
SspI	AATATT	1 cut(s)	2326
SspMI	CTAG	6 cut(s)	210, 722, 1223, 1280, 1674, 2552
StyD4I	CCNGG	4 cut(s)	84, 404, 848, 988
StyI	CCWWGG	1 cut(s)	2414
TaqI	TCGA	6 cut(s)	10, 964, 973, 1983, 2251, 2700
TatI	WGTACW	3 cut(s)	234, 790, 1350
TauI	GCSGC	2 cut(s)	2663, 2753
TfiI	GAWTC	8 cut(s)	476, 1015, 1495, 1988, 2003, 2141, 2563, 2683
TseFI	GTSAC	5 cut(s)	219, 375, 575, 1559, 1895
TseI	GCWGC	5 cut(s)	101, 882, 933, 1358, 2543
Tsp45I	GTSAC	5 cut(s)	219, 375, 575, 1559, 1895
TspGWI	ACGGA	3 cut(s)	292, 2237, 2635
Van91I	CCANNNNNTGG	1 cut(s)	2711
VneI	GTGCAC	2 cut(s)	34, 1916
VpaK11BI	GGWCC	4 cut(s)	630, 1814, 1839, 2418
XagI	CCTNNNNNAGG	1 cut(s)	2537
XbaI	TCTAGA	1 cut(s)	1279
XceI	RCATGY	3 cut(s)	645, 1894, 1926
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1482
XmiI	GTMKAC	3 cut(s)	1858, 1905, 2251
XmnI	GAANNNNTTC	2 cut(s)	621, 2217
XspI	CTAG	6 cut(s)	210, 722, 1223, 1280, 1674, 2552
Zsp2I	ATGCAT	1 cut(s)	1407

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.