pycom12g21000

39S ribosomal protein L45

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013393
Physical Location & Seq
Forward (+)
22285034 .. 22288500
3467 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 906 bp
ATGGCTTTCAGAAGTTTACACCGGATTCGAGCTCTCTATCGTACGGCCCAGATTGCAGAATCCTCCCATCTGCTTGGAAGCTCAAGAGGCTATGCCACTGGTATTTCAAATGTCCCTAGATTCTATTGCCGGAACGTATCGTCTTGCTTGTACAAGGGCAGCCATGGGGGCCTTCCTTGGACTCATGGAAGTTCTATAACATTGCGTTCTGCACTGGCTGTTGAGTTTCCGATTTTCTTGAGTGACACGAGGTCACTATCAACACAAGTAAAAGCCCCTGCACAAGCAAGACAAATGGGTGCTCTTAGAGTAGCCATGTTGAGTCCAGGTTTTATCTATGAGCCTTATACACCTCGTGAAAAAGTCTCATTTTTCAAGAGATGGTTCACAAGAAGCGGATGGAAAAGAACAAAAGAAGATATAATTATAGAGCTCAAAAGTGCATATGCCATTGCCAAATTAAGAAAAAAGGGGTACTCAAAGAATCTATTCTACAAAGAAGCAATAAACTTATACAAGGAGATAAATACTTTAATGGCAAACGGGGACAAAAATACTTTAAGGAAAGCAGTTACAGAGAAAATGTTTTCCGAACTAAAGAATGAAATAAAACAAAGAGATTCCATCTGGAGTAAGGTTTACTGGGAATTGGTGGAACCAGTTGTCAAAATACGAACTTTGCGAGCACGACTGATTGGCGTTGATCGAAATGATCTTGAGAAGGTGTTCATACAGCTTACGCTTGAGTTTCTGACAAAGCAGAAATTCGAAGCATATGACTCGAAGGGTGCTGTTGTTGCTGGAGATAAATCGAAGGAGGTTCTTGTTCGCGATATCTGGGTATTTGAAAAATCTCTCTTCCAGCCTAAAGCTTTCTGGCGTCTATGCGGGAGGATTTCAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim44

Protein Analysis

302

Amino Acids

34.66

Weight (kDa)

10.08

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim44 PF04280 147 - 297 6.4e-24 Tim44-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27395 AT5G27395
fragaria_vesca FvH4_6g05290
malus_domestica MD04G1209800.v1.1 MD12G1224400.v1.1
prunus_persica Prupe.6G330700_v2.0.a1 Prupe.6G330700_v2.0.a1
pyrus_communis pycom04g18600 pycom12g21000
rosa_chinensis RchiOBHm_Chr3g0454031
rosa_laevigata RLG00000025488
rosa_multiflora Rmu_sc0019265.1_g000003 Rmu_sc0020385.1_g000006
rosa_rugosa Rorug02G0652400
rosa_samantha Rh3AG055100 Rh3BG056700 Rh3DG057200
rosa_wichuraiana Rw3G004230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 831
AciI CCGC 2 cut(s) 396, 888
AcsI RAATTY 1 cut(s) 764
AcyI GRCGYC 1 cut(s) 880
AdeI CACNNNGTG 1 cut(s) 356
AfaI GTAC 3 cut(s) 43, 152, 476
AgsI TTSAA 3 cut(s) 108, 376, 848
AhdI GACNNNNNGTC 1 cut(s) 250
AjnI CCWGG 1 cut(s) 325
AluBI AGCT 5 cut(s) 32, 81, 433, 736, 872
AluI AGCT 5 cut(s) 32, 81, 433, 736, 872
Alw21I GWGCWC 4 cut(s) 34, 304, 435, 688
Alw26I GTCTC 1 cut(s) 370
AoxI GGCC 2 cut(s) 45, 169
ApeKI GCWGC 1 cut(s) 159
ApoI RAATTY 1 cut(s) 764
Asp700I GAANNNNTTC 2 cut(s) 488, 725
AspS9I GGNCC 2 cut(s) 46, 169
AsuII TTCGAA 1 cut(s) 768
BanII GRGCYC 2 cut(s) 34, 435
BauI CACGAG 2 cut(s) 247, 354
Bbv12I GWGCWC 4 cut(s) 34, 304, 435, 688
BbvI GCAGC 1 cut(s) 171
BccI CCATC 4 cut(s) 75, 375, 393, 632
BceAI ACGGC 1 cut(s) 60
BcgI CGANNNNNNTGC 2 cut(s) 762, 796
BciT130I CCWGG 1 cut(s) 327
BcoDI GTCTC 1 cut(s) 370
BfaI CTAG 1 cut(s) 117
BglI GCCNNNNNGGC 1 cut(s) 168
BisI GCNGC 1 cut(s) 160
BlsI GCNGC 1 cut(s) 161
Bme1390I CCNGG 1 cut(s) 327
BmeRI GACNNNNNGTC 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 46, 169
BmiI GGNNCC 2 cut(s) 170, 657
BmrFI CCNGG 1 cut(s) 327
BmrI ACTGGG 1 cut(s) 652
BmuI ACTGGG 1 cut(s) 652
BpmI CTGGAG 2 cut(s) 649, 822
Bpu14I TTCGAA 1 cut(s) 768
BpuEI CTTGAG 4 cut(s) 67, 259, 737, 764
BsaHI GRCGYC 1 cut(s) 880
BsaJI CCNNGG 2 cut(s) 163, 176
BsaWI WCCGGW 1 cut(s) 21
Bse1I ACTGG 4 cut(s) 103, 219, 647, 659
Bse3DI GCAATG 2 cut(s) 200, 450
BseBI CCWGG 1 cut(s) 327
BseDI CCNNGG 2 cut(s) 163, 176
BseGI GGATG 1 cut(s) 404
BseMI GCAATG 2 cut(s) 200, 450
BseNI ACTGG 4 cut(s) 103, 219, 647, 659
BseXI GCAGC 1 cut(s) 171
BsgI GTGCAG 2 cut(s) 195, 264
Bsh1236I CGCG 1 cut(s) 831
BshFI GGCC 2 cut(s) 47, 171
BsiHKAI GWGCWC 4 cut(s) 34, 304, 435, 688
BsiSI CCGG 2 cut(s) 22, 130
BsiWI CGTACG 1 cut(s) 41
BslFI GGGAC 2 cut(s) 98, 560
BsmAI GTCTC 1 cut(s) 370
BsmFI GGGAC 2 cut(s) 98, 560
BsnI GGCC 2 cut(s) 47, 171
Bsp119I TTCGAA 1 cut(s) 768
Bsp1286I GDGCHC 4 cut(s) 34, 304, 435, 688
Bsp1407I TGTACA 1 cut(s) 150
Bsp143I GATC 2 cut(s) 703, 712
Bsp19I CCATGG 1 cut(s) 163
Bsp68I TCGCGA 1 cut(s) 831
BspACI CCGC 2 cut(s) 396, 888
BspANI GGCC 2 cut(s) 47, 171
BspFNI CGCG 1 cut(s) 831
BspLI GGNNCC 2 cut(s) 170, 657
BspT104I TTCGAA 1 cut(s) 768
BsrDI GCAATG 2 cut(s) 200, 450
BsrGI TGTACA 1 cut(s) 150
BsrI ACTGG 4 cut(s) 103, 219, 647, 659
BssECI CCNNGG 2 cut(s) 163, 176
BssMI GATC 2 cut(s) 703, 712
BssNI GRCGYC 1 cut(s) 880
BssSI CACGAG 2 cut(s) 247, 354
BssT1I CCWWGG 2 cut(s) 163, 176
Bst2BI CACGAG 2 cut(s) 247, 354
Bst2UI CCWGG 1 cut(s) 327
Bst6I CTCTTC 1 cut(s) 863
BstACI GRCGYC 1 cut(s) 880
BstAUI TGTACA 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 768
BstC8I GCNNGC 1 cut(s) 684
BstDEI CTNAG 1 cut(s) 305
BstDSI CCRYGG 1 cut(s) 163
BstF5I GGATG 1 cut(s) 404
BstFNI CGCG 1 cut(s) 831
BstKTI GATC 2 cut(s) 706, 715
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 2 cut(s) 703, 712
BstMWI GCNNNNNNNGC 4 cut(s) 53, 87, 168, 797
BstNI CCWGG 1 cut(s) 327
BstSCI CCNGG 1 cut(s) 325
BstUI CGCG 1 cut(s) 831
BstV1I GCAGC 1 cut(s) 171
BstXI CCANNNNNNTGG 1 cut(s) 74
BsuRI GGCC 2 cut(s) 47, 171
BtgI CCRYGG 1 cut(s) 163
BtsCI GGATG 1 cut(s) 404
BtsIMutI CAGTG 2 cut(s) 96, 212
BtuMI TCGCGA 1 cut(s) 831
Cac8I GCNNGC 1 cut(s) 684
Cfr13I GGNCC 2 cut(s) 46, 169
CseI GACGC 1 cut(s) 869
Csp6I GTAC 3 cut(s) 42, 151, 475
CviAII CATG 3 cut(s) 164, 185, 316
CviQI GTAC 3 cut(s) 42, 151, 475
DdeI CTNAG 1 cut(s) 305
DpnI GATC 2 cut(s) 705, 714
DpnII GATC 2 cut(s) 703, 712
DraIII CACNNNGTG 1 cut(s) 356
DriI GACNNNNNGTC 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 863
Eam1105I GACNNNNNGTC 1 cut(s) 250
EarI CTCTTC 1 cut(s) 863
Ecl136II GAGCTC 2 cut(s) 32, 433
Eco130I CCWWGG 2 cut(s) 163, 176
Eco24I GRGCYC 2 cut(s) 34, 435
Eco32I GATATC 1 cut(s) 835
Eco53kI GAGCTC 2 cut(s) 32, 433
EcoICRI GAGCTC 2 cut(s) 32, 433
EcoO109I RGGNCCY 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 325
EcoRV GATATC 1 cut(s) 835
EcoT14I CCWWGG 2 cut(s) 163, 176
EcoT38I GRGCYC 2 cut(s) 34, 435
ErhI CCWWGG 2 cut(s) 163, 176
FaeI CATG 3 cut(s) 167, 188, 319
FaqI GGGAC 2 cut(s) 98, 560
FatI CATG 3 cut(s) 163, 184, 315
FauI CCCGC 1 cut(s) 881
FauNDI CATATG 2 cut(s) 445, 775
Fnu4HI GCNGC 1 cut(s) 160
FokI GGATG 1 cut(s) 411
FriOI GRGCYC 2 cut(s) 34, 435
Fsp4HI GCNGC 1 cut(s) 160
FspBI CTAG 1 cut(s) 117
GluI GCNGC 1 cut(s) 160
GsuI CTGGAG 2 cut(s) 649, 822
HaeIII GGCC 2 cut(s) 47, 171
HapII CCGG 2 cut(s) 22, 130
HgaI GACGC 1 cut(s) 869
Hin1I GRCGYC 1 cut(s) 880
Hin1II CATG 3 cut(s) 167, 188, 319
HindIII AAGCTT 1 cut(s) 870
HinfI GANTC 8 cut(s) 25, 59, 120, 181, 322, 484, 620, 779
HpaII CCGG 2 cut(s) 22, 130
Hpy166II GTNNAC 3 cut(s) 17, 387, 640
Hpy188I TCNGA 4 cut(s) 11, 231, 592, 753
Hpy188III TCNNGA 7 cut(s) 84, 238, 356, 376, 628, 716, 830
Hpy8I GTNNAC 3 cut(s) 17, 387, 640
HpyAV CCTTC 4 cut(s) 182, 715, 778, 808
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 4 cut(s) 56, 212, 281, 443
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 87, 168, 797
HpyF3I CTNAG 1 cut(s) 305
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 880
Hsp92II CATG 3 cut(s) 167, 188, 319
Kzo9I GATC 2 cut(s) 703, 712
Lsp1109I GCAGC 1 cut(s) 171
MaeI CTAG 1 cut(s) 117
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 3 cut(s) 242, 252, 571
MalI GATC 2 cut(s) 705, 714
MboI GATC 2 cut(s) 703, 712
MboII GAAGA 2 cut(s) 428, 850
MhlI GDGCHC 4 cut(s) 34, 304, 435, 688
MluCI AATT 4 cut(s) 423, 458, 647, 764
MlyI GAGTC 3 cut(s) 175, 331, 773
MnlI CCTC 6 cut(s) 73, 80, 243, 363, 811, 885
MroXI GAANNNNTTC 2 cut(s) 488, 725
MseI TTAA 3 cut(s) 461, 533, 560
MspI CCGG 2 cut(s) 22, 130
MspR9I CCNGG 1 cut(s) 327
MvaI CCWGG 1 cut(s) 327
MvnI CGCG 1 cut(s) 831
MwoI GCNNNNNNNGC 4 cut(s) 53, 87, 168, 797
NcoI CCATGG 1 cut(s) 163
NdeI CATATG 2 cut(s) 445, 775
NdeII GATC 2 cut(s) 703, 712
NlaIII CATG 3 cut(s) 167, 188, 319
NlaIV GGNNCC 2 cut(s) 170, 657
NmuCI GTSAC 2 cut(s) 242, 252
NruI TCGCGA 1 cut(s) 831
NspV TTCGAA 1 cut(s) 768
PcsI WCGNNNNNNNCGW 1 cut(s) 679
PdmI GAANNNNTTC 2 cut(s) 488, 725
PfeI GAWTC 5 cut(s) 25, 59, 120, 484, 620
Pfl23II CGTACG 1 cut(s) 41
PkrI GCNGC 1 cut(s) 161
PleI GAGTC 3 cut(s) 175, 330, 773
PpsI GAGTC 3 cut(s) 175, 330, 773
Psp124BI GAGCTC 2 cut(s) 34, 435
Psp6I CCWGG 1 cut(s) 325
PspGI CCWGG 1 cut(s) 325
PspLI CGTACG 1 cut(s) 41
PspN4I GGNNCC 2 cut(s) 170, 657
PspPI GGNCC 2 cut(s) 46, 169
RruI TCGCGA 1 cut(s) 831
RsaI GTAC 3 cut(s) 43, 152, 476
RsaNI GTAC 3 cut(s) 42, 151, 475
SacI GAGCTC 2 cut(s) 34, 435
SaqAI TTAA 3 cut(s) 461, 533, 560
SatI GCNGC 1 cut(s) 160
Sau3AI GATC 2 cut(s) 703, 712
Sau96I GGNCC 2 cut(s) 46, 169
SchI GAGTC 3 cut(s) 175, 331, 773
ScrFI CCNGG 1 cut(s) 327
SduI GDGCHC 4 cut(s) 34, 304, 435, 688
SfuI TTCGAA 1 cut(s) 768
SmlI CTYRAG 4 cut(s) 82, 238, 716, 743
SmoI CTYRAG 4 cut(s) 82, 238, 716, 743
Sse9I AATT 4 cut(s) 423, 458, 647, 764
SsiI CCGC 2 cut(s) 396, 888
SspMI CTAG 1 cut(s) 117
SstI GAGCTC 2 cut(s) 34, 435
StyD4I CCNGG 1 cut(s) 325
StyI CCWWGG 2 cut(s) 163, 176
TaiI ACGT 1 cut(s) 138
TaqI TCGA 5 cut(s) 28, 706, 768, 782, 812
TasI AATT 4 cut(s) 423, 458, 647, 764
TatI WGTACW 1 cut(s) 150
TfiI GAWTC 5 cut(s) 25, 59, 120, 484, 620
Tru1I TTAA 3 cut(s) 461, 533, 560
Tru9I TTAA 3 cut(s) 461, 533, 560
TscAI CASTG 2 cut(s) 103, 219
TseFI GTSAC 2 cut(s) 242, 252
TseI GCWGC 1 cut(s) 159
Tsp45I GTSAC 2 cut(s) 242, 252
TspDTI ATGAA 2 cut(s) 618, 718
TspRI CASTG 2 cut(s) 103, 219
XapI RAATTY 1 cut(s) 764
XmnI GAANNNNTTC 2 cut(s) 488, 725
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.