pycom12g21330

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0017564
Physical Location & Seq
Reverse (-)
22515930 .. 22516845
916 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 699 bp
ATGGCTCAAAAGGCACAACGACTCTTCCCTGGGCTTGTGAGCATTGAGAAATTACTTACAAAACAATACGGGAAGCTGGCCTTGTTACTTCATCTTGATAAGAATAAGTTTGCTGGTGCGGAGGCGGCTTTTAAGTTGATCGGGGAAGCAGTAAGCGTGATGGCAGACCAAGCAAAGCGCTCTACGTATGACTTGAAGCGTAGAGCTCAAGTGAAAACTGGTTCTTCACCATCAGCTCAACCGACAAATGGAAACTTATTTGTCAGGAAACAAAACAATACTCCTCAGTCACGGTTCCCACCAGACACATTCTGGACACGTTGTCCTTCTTGGTGCTCATCCAGAATCTGCCTGGAATATCAACTTCCAAATCAAAAAGAACCTAAAAGTCAGGGTACTTCAAATGCAGCCCCACAAAGTAATGGTGGCACTGGAAAACCATCCTCCGCACGATTTAATAACGGAAAGGCAGCCTCAAATCCGGCACCAAAGGTGGGAGTTGCTGCTGATGTTAAGATGTCCAAGTCTGGTCCTGTGAAGTCCAAGGGTTCAAAAACTTCAGGAAACATGAACAATATTAAAAGAGGAAGGGAACCAGTGAAAGTTGACTACAAGAAGGATAAGTACGTGAAAACTGAAAAATCTCTTAAACACTGTGTTCCAAGTTCAAACTCTCATCAATTTTTTAGTGCAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

233

Amino Acids

25.29

Weight (kDa)

10.16

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 19 - 64 3.2e-09 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 484
AciI CCGC 3 cut(s) 119, 125, 447
AcuI CTGAAG 1 cut(s) 543
AfaI GTAC 2 cut(s) 397, 626
AfeI AGCGCT 1 cut(s) 179
AfiI CCNNNNNNNGG 2 cut(s) 248, 494
AflIII ACRYGT 1 cut(s) 317
AgsI TTSAA 4 cut(s) 196, 402, 552, 669
AhdI GACNNNNNGTC 1 cut(s) 321
AjnI CCWGG 2 cut(s) 28, 351
AluBI AGCT 3 cut(s) 76, 206, 236
AluI AGCT 3 cut(s) 76, 206, 236
Alw21I GWGCWC 2 cut(s) 208, 338
AlwNI CAGNNNCTG 1 cut(s) 348
Aor51HI AGCGCT 1 cut(s) 179
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 3 cut(s) 407, 470, 503
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 1 cut(s) 530
AsuHPI GGTGA 1 cut(s) 219
AvaII GGWCC 1 cut(s) 530
BanI GGYRCC 1 cut(s) 484
BanII GRGCYC 1 cut(s) 208
BarI GAAGNNNNNNTAC 2 cut(s) 608, 640
Bbv12I GWGCWC 2 cut(s) 208, 338
BbvI GCAGC 3 cut(s) 419, 482, 490
BccI CCATC 3 cut(s) 154, 238, 448
BciT130I CCWGG 2 cut(s) 30, 353
BfoI RGCGCY 1 cut(s) 181
BisI GCNGC 4 cut(s) 126, 408, 471, 504
BlsI GCNGC 4 cut(s) 127, 409, 472, 505
Bme1390I CCNGG 2 cut(s) 30, 353
Bme18I GGWCC 1 cut(s) 530
BmeRI GACNNNNNGTC 1 cut(s) 321
BmgT120I GGNCC 1 cut(s) 530
BmiI GGNNCC 3 cut(s) 296, 486, 594
BmrFI CCNGG 2 cut(s) 30, 353
BpuEI CTTGAG 1 cut(s) 192
BsaAI YACGTR 2 cut(s) 186, 628
BsaJI CCNNGG 3 cut(s) 28, 29, 543
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 494
Bse1I ACTGG 3 cut(s) 223, 436, 596
BseBI CCWGG 2 cut(s) 30, 353
BseDI CCNNGG 3 cut(s) 28, 29, 543
BseGI GGATG 2 cut(s) 338, 440
BseLI CCNNNNNNNGG 2 cut(s) 248, 494
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 3 cut(s) 223, 436, 596
BseRI GAGGAG 1 cut(s) 273
BseXI GCAGC 3 cut(s) 419, 482, 490
BshFI GGCC 1 cut(s) 80
BshNI GGYRCC 1 cut(s) 484
BsiHKAI GWGCWC 2 cut(s) 208, 338
BsiSI CCGG 1 cut(s) 482
BslI CCNNNNNNNGG 2 cut(s) 248, 494
BsnI GGCC 1 cut(s) 80
Bsp1286I GDGCHC 2 cut(s) 208, 338
Bsp143I GATC 1 cut(s) 138
BspACI CCGC 3 cut(s) 119, 125, 447
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 1 cut(s) 298
BspLI GGNNCC 3 cut(s) 296, 486, 594
BspT107I GGYRCC 1 cut(s) 484
BsrI ACTGG 3 cut(s) 223, 436, 596
BssECI CCNNGG 3 cut(s) 28, 29, 543
BssMI GATC 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 543
Bst2UI CCWGG 2 cut(s) 30, 353
Bst4CI ACNGT 2 cut(s) 294, 656
Bst6I CTCTTC 1 cut(s) 29
BstBAI YACGTR 2 cut(s) 186, 628
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 1 cut(s) 285
BstF5I GGATG 2 cut(s) 338, 440
BstH2I RGCGCY 1 cut(s) 181
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 1 cut(s) 141
BstMBI GATC 1 cut(s) 138
BstMWI GCNNNNNNNGC 3 cut(s) 11, 125, 170
BstNI CCWGG 2 cut(s) 30, 353
BstSCI CCNGG 2 cut(s) 28, 351
BstSNI TACGTA 1 cut(s) 186
BstV1I GCAGC 3 cut(s) 419, 482, 490
BsuRI GGCC 1 cut(s) 80
BtsCI GGATG 2 cut(s) 338, 440
BtsIMutI CAGTG 3 cut(s) 429, 603, 652
Cac8I GCNNGC 1 cut(s) 78
CaiI CAGNNNCTG 1 cut(s) 348
CfoI GCGC 1 cut(s) 180
Cfr13I GGNCC 1 cut(s) 530
Csp6I GTAC 2 cut(s) 396, 625
CviAII CATG 1 cut(s) 568
CviJI RGCY 9 cut(s) 5, 34, 76, 80, 128, 206, 236, 410, 473
CviKI_1 RGCY 9 cut(s) 5, 34, 76, 80, 128, 206, 236, 410, 473
CviQI GTAC 2 cut(s) 396, 625
DdeI CTNAG 1 cut(s) 285
DpnI GATC 1 cut(s) 140
DpnII GATC 1 cut(s) 138
DriI GACNNNNNGTC 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 29
Eam1105I GACNNNNNGTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 29
Ecl136II GAGCTC 1 cut(s) 206
Eco105I TACGTA 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 208
Eco47I GGWCC 1 cut(s) 530
Eco47III AGCGCT 1 cut(s) 179
Eco53kI GAGCTC 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 543
EcoICRI GAGCTC 1 cut(s) 206
EcoRII CCWGG 2 cut(s) 28, 351
EcoT14I CCWWGG 1 cut(s) 543
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 543
FaeI CATG 1 cut(s) 571
FaiI YATR 2 cut(s) 189, 569
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FatI CATG 1 cut(s) 567
Fnu4HI GCNGC 4 cut(s) 126, 408, 471, 504
FokI GGATG 2 cut(s) 325, 427
FriOI GRGCYC 1 cut(s) 208
Fsp4HI GCNGC 4 cut(s) 126, 408, 471, 504
GlaI GCGC 1 cut(s) 179
GluI GCNGC 4 cut(s) 126, 408, 471, 504
HaeII RGCGCY 1 cut(s) 181
HaeIII GGCC 1 cut(s) 80
HapII CCGG 1 cut(s) 482
HhaI GCGC 1 cut(s) 180
Hin1II CATG 1 cut(s) 571
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HinfI GANTC 2 cut(s) 21, 345
HpaII CCGG 1 cut(s) 482
HphI GGTGA 1 cut(s) 219
Hpy166II GTNNAC 1 cut(s) 607
Hpy188III TCNNGA 5 cut(s) 95, 265, 313, 342, 561
Hpy8I GTNNAC 1 cut(s) 607
HpyAV CCTTC 3 cut(s) 336, 582, 610
HpyCH4III ACNGT 2 cut(s) 294, 656
HpyCH4IV ACGT 3 cut(s) 185, 319, 627
HpyCH4V TGCA 2 cut(s) 407, 692
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 125, 170
HpyF3I CTNAG 1 cut(s) 285
HpySE526I ACGT 3 cut(s) 185, 319, 627
Hsp92II CATG 1 cut(s) 571
HspAI GCGC 1 cut(s) 178
Kzo9I GATC 1 cut(s) 138
Lsp1109I GCAGC 3 cut(s) 419, 482, 490
MaeII ACGT 3 cut(s) 185, 319, 627
MaeIII GTNAC 2 cut(s) 84, 288
MalI GATC 1 cut(s) 140
MboI GATC 1 cut(s) 138
MboII GAAGA 2 cut(s) 16, 216
MhlI GDGCHC 2 cut(s) 208, 338
MluCI AATT 2 cut(s) 50, 680
MlyI GAGTC 1 cut(s) 15
MnlI CCTC 5 cut(s) 115, 294, 454, 484, 578
MseI TTAA 5 cut(s) 132, 456, 513, 579, 648
MspI CCGG 1 cut(s) 482
MspR9I CCNGG 2 cut(s) 30, 353
MvaI CCWGG 2 cut(s) 30, 353
MwoI GCNNNNNNNGC 3 cut(s) 11, 125, 170
NdeII GATC 1 cut(s) 138
NlaIII CATG 1 cut(s) 571
NlaIV GGNNCC 3 cut(s) 296, 486, 594
NmuCI GTSAC 1 cut(s) 288
PasI CCCWGGG 1 cut(s) 29
PfeI GAWTC 1 cut(s) 345
PkrI GCNGC 4 cut(s) 127, 409, 472, 505
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
Ppu21I YACGTR 2 cut(s) 186, 628
Psp124BI GAGCTC 1 cut(s) 208
Psp6I CCWGG 2 cut(s) 28, 351
PspGI CCWGG 2 cut(s) 28, 351
PspN4I GGNNCC 3 cut(s) 296, 486, 594
PspPI GGNCC 1 cut(s) 530
PstNI CAGNNNCTG 1 cut(s) 348
RsaI GTAC 2 cut(s) 397, 626
RsaNI GTAC 2 cut(s) 396, 625
SacI GAGCTC 1 cut(s) 208
SaqAI TTAA 5 cut(s) 132, 456, 513, 579, 648
SatI GCNGC 4 cut(s) 126, 408, 471, 504
Sau3AI GATC 1 cut(s) 138
Sau96I GGNCC 1 cut(s) 530
SchI GAGTC 1 cut(s) 15
ScrFI CCNGG 2 cut(s) 30, 353
SduI GDGCHC 2 cut(s) 208, 338
SetI ASST 8 cut(s) 78, 188, 208, 238, 322, 385, 495, 630
SinI GGWCC 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 207
SmoI CTYRAG 1 cut(s) 207
SnaBI TACGTA 1 cut(s) 186
Sse9I AATT 2 cut(s) 50, 680
SsiI CCGC 3 cut(s) 119, 125, 447
SspI AATATT 1 cut(s) 577
SstI GAGCTC 1 cut(s) 208
StyD4I CCNGG 2 cut(s) 28, 351
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 2 cut(s) 294, 656
TaiI ACGT 3 cut(s) 188, 322, 630
TasI AATT 2 cut(s) 50, 680
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 1 cut(s) 345
Tru1I TTAA 5 cut(s) 132, 456, 513, 579, 648
Tru9I TTAA 5 cut(s) 132, 456, 513, 579, 648
TscAI CASTG 3 cut(s) 436, 603, 659
TseFI GTSAC 1 cut(s) 288
TseI GCWGC 3 cut(s) 407, 470, 503
Tsp45I GTSAC 1 cut(s) 288
TspDTI ATGAA 2 cut(s) 80, 584
TspGWI ACGGA 1 cut(s) 477
TspRI CASTG 3 cut(s) 436, 603, 659
VpaK11BI GGWCC 1 cut(s) 530
XcmI CCANNNNNNNNNTGG 2 cut(s) 309, 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.