pycom12g22050

Calcium-binding EF hand family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014521
Physical Location & Seq
Forward (+)
23090492 .. 23093594
3103 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 963 bp
ATGTCGGACGGGGCTTTAACGGTCCTCGACGGGAGCCACCTGAGAGCCGTCGACCTTACTCTGCCGGAAGCTGACGCCAGCTTAACGGGAGCTCAGGTCCTCGACCTCGCCGTCTCTAAAACCTCTTCCTCCCTCTTCGGCCTCTCGCTGCCTCAGAGCCTCAAGTCCTCTGCTTTGAGACGCATCAGCCTCCAAGAAGACGACGGTTTCCGAAGCACGGAGCTCGATCGCGCCCAGGCTCTGAAGGTGATCGGCGAGTACATCACGGCGATCGCCGATGAATTGAAAGATGATCCACTTGTCGTATCTGTCTTGGACGGATACACTATTCGATTGTTTTTAGAGGACGAGGATGATTTTGCAATGTTAGCAGAGAATCTGTTCACTGATCTGGATCTAGAAGATAAAGGAAAGATCAACAAGAATGAGATCCGGAATGCGCTTGTTCACATGGGAGTTGAGATGGGCGTTCCTCCTGTTTCAGTTCCTCCCCTAAGTGATATCTTGATGAAACACGAAGCTGATGGGGATGAAGAACTGGGCCAAGCACAATTTGCTCAGCTACTCCAGCCTGTCCTGCAGGAACTAGCCGAGGCCTTGGCTAAAAAGCATGTTGTTTTTATCCAGAGCATCAAGATTGTCAACGGTTCTCAGCTAAGAAAGCTTTTGGCTGATGAGAAGCAATTGAACAATGTCGTTGAGAAGATATTGCAAGAAGATCACCATGGAAAGGACGGATTGGGGAATGCAGAAAAAATTAGGAGTTTTCTTGTGAAAAATGGGCAAGAGTTGGGTCTGCCACCATACGAGGCCGAGGCAGTTGATCTTTTGTATGATGCAGTGTTTGATGATCTAGACGCAGACAAGAGTGCTCCGGAAGACAAATGTGGAAATCTTGTGAAGGAGATACTCGAGAAATTCGCAGATCAGCTGGAAGCGAGTCCTGTTTTTTACAATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

321

Amino Acids

35.01

Weight (kDa)

4.44

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014521)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G28830
fragaria_vesca FvH4_6g02990
malus_domestica MD04G1224000.v1.1 MD12G1240800.v1.1
prunus_persica Prupe.6G342700_v2.0.a1
pyrus_communis pycom04g19740 pycom12g22050
rosa_chinensis RchiOBHm_Chr3g0451171
rosa_laevigata RLG00000025680
rosa_multiflora Rmu_co8512575.1_g000001
rosa_roxburghii Rroxscaffold_6G00428640
rosa_rugosa Rorug02G0632500
rosa_samantha Rh3AG035000 Rh3BG035400 Rh3CG034000 Rh3DG035000
rosa_wichuraiana Rw3G002570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 51
AccII CGCG 1 cut(s) 231
AccIII TCCGGA 2 cut(s) 432, 874
AclWI GGATC 3 cut(s) 287, 402, 424
AcsI RAATTY 1 cut(s) 917
AcuI CTGAAG 1 cut(s) 263
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 2 cut(s) 217, 730
AgsI TTSAA 2 cut(s) 286, 688
AjnI CCWGG 1 cut(s) 234
AluBI AGCT 9 cut(s) 71, 81, 92, 223, 521, 562, 655, 664, 931
AluI AGCT 9 cut(s) 71, 81, 92, 223, 521, 562, 655, 664, 931
Alw21I GWGCWC 3 cut(s) 94, 225, 874
Alw26I GTCTC 2 cut(s) 118, 172
AlwI GGATC 3 cut(s) 287, 402, 424
AlwNI CAGNNNCTG 1 cut(s) 241
Ama87I CYCGRG 1 cut(s) 911
Aor13HI TCCGGA 2 cut(s) 432, 874
AoxI GGCC 4 cut(s) 139, 541, 594, 810
ApeKI GCWGC 1 cut(s) 148
ApoI RAATTY 1 cut(s) 917
AsiSI GCGATCGC 1 cut(s) 273
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 2 cut(s) 233, 442
AspS9I GGNCC 3 cut(s) 22, 97, 541
AsuHPI GGTGA 2 cut(s) 259, 713
AvaI CYCGRG 1 cut(s) 911
AvaII GGWCC 2 cut(s) 22, 97
BanII GRGCYC 2 cut(s) 94, 225
BbsI GAAGAC 2 cut(s) 204, 885
Bbv12I GWGCWC 3 cut(s) 94, 225, 874
BbvI GCAGC 1 cut(s) 135
BccI CCATC 2 cut(s) 457, 518
BceAI ACGGC 3 cut(s) 32, 95, 282
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BciT130I CCWGG 1 cut(s) 236
BciVI GTATCC 1 cut(s) 314
BcoDI GTCTC 2 cut(s) 118, 172
BfaI CTAG 3 cut(s) 398, 587, 854
BfmI CTRYAG 1 cut(s) 578
BfuI GTATCC 1 cut(s) 314
BisI GCNGC 1 cut(s) 149
BlpI GCTNAGC 1 cut(s) 558
BlsI GCNGC 1 cut(s) 150
Bme1390I CCNGG 1 cut(s) 236
Bme18I GGWCC 2 cut(s) 22, 97
BmeT110I CYCGRG 1 cut(s) 911
BmgT120I GGNCC 3 cut(s) 22, 97, 541
BmiI GGNNCC 1 cut(s) 35
BmrFI CCNGG 1 cut(s) 236
BmrI ACTGGG 1 cut(s) 548
BmsI GCATC 3 cut(s) 192, 639, 826
BmuI ACTGGG 1 cut(s) 548
BpiI GAAGAC 2 cut(s) 204, 885
BpmI CTGGAG 1 cut(s) 551
Bpu10I CCTNAGC 1 cut(s) 93
Bpu1102I GCTNAGC 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 146
BsaBI GATNNNNATC 1 cut(s) 393
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 5 cut(s) 234, 591, 597, 724, 813
BsaWI WCCGGW 2 cut(s) 432, 874
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 730
Bse1I ACTGG 1 cut(s) 543
Bse3DI GCAATG 1 cut(s) 369
Bse8I GATNNNNATC 1 cut(s) 393
BseAI TCCGGA 2 cut(s) 432, 874
BseBI CCWGG 1 cut(s) 236
BseDI CCNNGG 5 cut(s) 234, 591, 597, 724, 813
BseGI GGATG 2 cut(s) 358, 535
BseJI GATNNNNATC 1 cut(s) 393
BseLI CCNNNNNNNGG 2 cut(s) 217, 730
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 5 cut(s) 32, 107, 167, 572, 665
BseNI ACTGG 1 cut(s) 543
BseXI GCAGC 1 cut(s) 135
Bsh1236I CGCG 1 cut(s) 231
Bsh1285I CGRYCG 2 cut(s) 229, 273
BshFI GGCC 4 cut(s) 141, 543, 596, 812
BsiEI CGRYCG 2 cut(s) 229, 273
BsiHKAI GWGCWC 3 cut(s) 94, 225, 874
BsiHKCI CYCGRG 1 cut(s) 911
BsiSI CCGG 3 cut(s) 65, 433, 875
BslI CCNNNNNNNGG 2 cut(s) 217, 730
BsmAI GTCTC 2 cut(s) 118, 172
BsmBI CGTCTC 2 cut(s) 118, 172
BsmI GAATGC 2 cut(s) 442, 751
BsnI GGCC 4 cut(s) 141, 543, 596, 812
BsoBI CYCGRG 1 cut(s) 911
Bsp1286I GDGCHC 3 cut(s) 94, 225, 874
Bsp13I TCCGGA 2 cut(s) 432, 874
Bsp1720I GCTNAGC 1 cut(s) 558
Bsp19I CCATGG 1 cut(s) 724
BspANI GGCC 4 cut(s) 141, 543, 596, 812
BspCNI CTCAG 5 cut(s) 33, 106, 166, 571, 664
BspEI TCCGGA 2 cut(s) 432, 874
BspFNI CGCG 1 cut(s) 231
BspLI GGNNCC 1 cut(s) 35
BspMAI CTGCAG 1 cut(s) 582
BspPI GGATC 3 cut(s) 287, 402, 424
BsrDI GCAATG 1 cut(s) 369
BsrI ACTGG 1 cut(s) 543
BssECI CCNNGG 5 cut(s) 234, 591, 597, 724, 813
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 2 cut(s) 597, 724
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 3 cut(s) 22, 206, 647
Bst6I CTCTTC 2 cut(s) 130, 140
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 1 cut(s) 554
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 7 cut(s) 41, 93, 153, 494, 558, 651, 656
BstDSI CCRYGG 1 cut(s) 724
BstF5I GGATG 2 cut(s) 358, 535
BstFNI CGCG 1 cut(s) 231
BstHHI GCGC 2 cut(s) 233, 442
BstMAI GTCTC 2 cut(s) 118, 172
BstMCI CGRYCG 2 cut(s) 229, 273
BstMWI GCNNNNNNNGC 5 cut(s) 368, 554, 568, 577, 661
BstNI CCWGG 1 cut(s) 236
BstNSI RCATGY 1 cut(s) 614
BstSCI CCNGG 1 cut(s) 234
BstSFI CTRYAG 1 cut(s) 578
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 1 cut(s) 135
BstV2I GAAGAC 2 cut(s) 204, 885
BstX2I RGATCY 2 cut(s) 394, 429
BstYI RGATCY 2 cut(s) 394, 429
BsuI GTATCC 1 cut(s) 314
BsuRI GGCC 4 cut(s) 141, 543, 596, 812
BtgI CCRYGG 1 cut(s) 724
BtsCI GGATG 2 cut(s) 358, 535
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 2 cut(s) 384, 846
Cac8I GCNNGC 1 cut(s) 79
CaiI CAGNNNCTG 1 cut(s) 241
CfoI GCGC 2 cut(s) 233, 442
Cfr13I GGNCC 3 cut(s) 22, 97, 541
CseI GACGC 3 cut(s) 83, 189, 866
Csp6I GTAC 1 cut(s) 259
CviAII CATG 3 cut(s) 451, 611, 725
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 7 cut(s) 41, 93, 153, 494, 558, 651, 656
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
Eam1104I CTCTTC 2 cut(s) 130, 140
EarI CTCTTC 2 cut(s) 130, 140
Ecl136II GAGCTC 2 cut(s) 92, 223
Eco130I CCWWGG 2 cut(s) 597, 724
Eco147I AGGCCT 1 cut(s) 596
Eco24I GRGCYC 2 cut(s) 94, 225
Eco32I GATATC 1 cut(s) 502
Eco47I GGWCC 2 cut(s) 22, 97
Eco53kI GAGCTC 2 cut(s) 92, 223
Eco57I CTGAAG 1 cut(s) 263
Eco88I CYCGRG 1 cut(s) 911
EcoICRI GAGCTC 2 cut(s) 92, 223
EcoO109I RGGNCCY 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 234
EcoRV GATATC 1 cut(s) 502
EcoT14I CCWWGG 2 cut(s) 597, 724
EcoT38I GRGCYC 2 cut(s) 94, 225
ErhI CCWWGG 2 cut(s) 597, 724
Esp3I CGTCTC 2 cut(s) 118, 172
FaeI CATG 3 cut(s) 454, 614, 728
FaiI YATR 5 cut(s) 452, 612, 726, 805, 834
FatI CATG 3 cut(s) 450, 610, 724
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 1 cut(s) 149
FokI GGATG 2 cut(s) 365, 542
FriOI GRGCYC 2 cut(s) 94, 225
Fsp4HI GCNGC 1 cut(s) 149
FspBI CTAG 3 cut(s) 398, 587, 854
GlaI GCGC 2 cut(s) 232, 441
GluI GCNGC 1 cut(s) 149
GsuI CTGGAG 1 cut(s) 551
HaeIII GGCC 4 cut(s) 141, 543, 596, 812
HapII CCGG 3 cut(s) 65, 433, 875
HgaI GACGC 3 cut(s) 83, 189, 866
HhaI GCGC 2 cut(s) 233, 442
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 3 cut(s) 454, 614, 728
Hin6I GCGC 2 cut(s) 231, 440
HinP1I GCGC 2 cut(s) 231, 440
HincII GTYRAC 2 cut(s) 52, 643
HindII GTYRAC 2 cut(s) 52, 643
HindIII AAGCTT 1 cut(s) 662
HinfI GANTC 2 cut(s) 376, 940
HpaII CCGG 3 cut(s) 65, 433, 875
HphI GGTGA 2 cut(s) 259, 713
Hpy166II GTNNAC 4 cut(s) 52, 384, 448, 643
Hpy188I TCNGA 4 cut(s) 7, 156, 212, 243
Hpy188III TCNNGA 9 cut(s) 392, 398, 433, 505, 625, 634, 854, 875, 913
Hpy8I GTNNAC 4 cut(s) 52, 384, 448, 643
Hpy99I CGWCG 3 cut(s) 32, 53, 206
HpyAV CCTTC 2 cut(s) 238, 895
HpyCH4III ACNGT 3 cut(s) 22, 206, 647
HpyCH4V TGCA 5 cut(s) 362, 580, 712, 749, 839
HpyF10VI GCNNNNNNNGC 5 cut(s) 368, 554, 568, 577, 661
HpyF3I CTNAG 7 cut(s) 41, 93, 153, 494, 558, 651, 656
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 3 cut(s) 454, 614, 728
HspAI GCGC 2 cut(s) 231, 440
Kpn2I TCCGGA 2 cut(s) 432, 874
LmnI GCTCC 4 cut(s) 33, 89, 220, 877
Lsp1109I GCAGC 1 cut(s) 135
LweI GCATC 3 cut(s) 192, 639, 826
MaeI CTAG 3 cut(s) 398, 587, 854
MboII GAAGA 8 cut(s) 117, 127, 209, 413, 545, 715, 728, 890
MfeI CAATTG 1 cut(s) 683
MflI RGATCY 2 cut(s) 394, 429
MhlI GDGCHC 3 cut(s) 94, 225, 874
MluCI AATT 5 cut(s) 281, 551, 683, 756, 917
MlyI GAGTC 1 cut(s) 949
MroI TCCGGA 2 cut(s) 432, 874
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 2 cut(s) 17, 83
MspA1I CMGCKG 1 cut(s) 931
MspI CCGG 3 cut(s) 65, 433, 875
MspR9I CCNGG 1 cut(s) 236
MunI CAATTG 1 cut(s) 683
Mva1269I GAATGC 2 cut(s) 442, 751
MvaI CCWGG 1 cut(s) 236
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 5 cut(s) 368, 554, 568, 577, 661
NcoI CCATGG 1 cut(s) 724
NlaIII CATG 3 cut(s) 454, 614, 728
NlaIV GGNNCC 1 cut(s) 35
NmeAIII GCCGAG 2 cut(s) 616, 838
NspI RCATGY 1 cut(s) 614
PaeR7I CTCGAG 1 cut(s) 911
PceI AGGCCT 1 cut(s) 596
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PctI GAATGC 2 cut(s) 442, 751
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 376
PkrI GCNGC 1 cut(s) 150
Ple19I CGATCG 2 cut(s) 229, 273
PleI GAGTC 1 cut(s) 948
PpsI GAGTC 1 cut(s) 948
PpuMI RGGWCCY 1 cut(s) 97
Psp124BI GAGCTC 2 cut(s) 94, 225
Psp5II RGGWCCY 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 234
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 1 cut(s) 35
PspPI GGNCC 3 cut(s) 22, 97, 541
PspPPI RGGWCCY 1 cut(s) 97
PstI CTGCAG 1 cut(s) 582
PstNI CAGNNNCTG 1 cut(s) 241
PsuI RGATCY 2 cut(s) 394, 429
PvuI CGATCG 2 cut(s) 229, 273
PvuII CAGCTG 1 cut(s) 931
RgaI GCGATCGC 1 cut(s) 273
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SacI GAGCTC 2 cut(s) 94, 225
SalI GTCGAC 1 cut(s) 50
SaqAI TTAA 2 cut(s) 17, 83
SatI GCNGC 1 cut(s) 149
Sau96I GGNCC 3 cut(s) 22, 97, 541
SbfI CCTGCAGG 1 cut(s) 582
SchI GAGTC 1 cut(s) 949
ScrFI CCNGG 1 cut(s) 236
SdaI CCTGCAGG 1 cut(s) 582
SduI GDGCHC 3 cut(s) 94, 225, 874
SfaAI GCGATCGC 1 cut(s) 273
SfaNI GCATC 3 cut(s) 192, 639, 826
SfcI CTRYAG 1 cut(s) 578
Sfr274I CTCGAG 1 cut(s) 911
SgfI GCGATCGC 1 cut(s) 273
SinI GGWCC 2 cut(s) 22, 97
SlaI CTCGAG 1 cut(s) 911
SmlI CTYRAG 2 cut(s) 161, 911
SmoI CTYRAG 2 cut(s) 161, 911
Sse8387I CCTGCAGG 1 cut(s) 582
Sse9I AATT 5 cut(s) 281, 551, 683, 756, 917
SseBI AGGCCT 1 cut(s) 596
SspMI CTAG 3 cut(s) 398, 587, 854
SstI GAGCTC 2 cut(s) 94, 225
StuI AGGCCT 1 cut(s) 596
StyD4I CCNGG 1 cut(s) 234
StyI CCWWGG 2 cut(s) 597, 724
TaaI ACNGT 3 cut(s) 22, 206, 647
TaqI TCGA 6 cut(s) 27, 51, 102, 225, 331, 912
TasI AATT 5 cut(s) 281, 551, 683, 756, 917
TatI WGTACW 1 cut(s) 258
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 2 cut(s) 17, 83
Tru9I TTAA 2 cut(s) 17, 83
TscAI CASTG 2 cut(s) 391, 846
TseI GCWGC 1 cut(s) 148
TspDTI ATGAA 3 cut(s) 294, 524, 546
TspGWI ACGGA 3 cut(s) 233, 333, 750
TspRI CASTG 2 cut(s) 391, 846
VpaK11BI GGWCC 2 cut(s) 22, 97
XapI RAATTY 1 cut(s) 917
XbaI TCTAGA 2 cut(s) 397, 853
XceI RCATGY 1 cut(s) 614
XhoI CTCGAG 1 cut(s) 911
XmiI GTMKAC 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 3 cut(s) 398, 587, 854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.