Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0009239

Physical Location & Seq

Genomic Coordinates Reverse (-)

23127353 .. 23135366

Gene Structure 8014 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5412 bp

ATGGCGTCGGAGCTAGGGCAACAGACGGTGGAGTTCTCCGCCCTGGTGAGCCGCGCCGCCGAGGAATCCTTCCTCGCGCTGAAAGAGCTCACAGACAAATCGAAGGCGTCGCCGGACCAGTCCGATACCGATAAGAAAATTGGACTGCTCAAGTACTTGGCTAAGACCCAGCAACGCATGCTCCGGCTCAATGTCCTCGCCAAGTGGTGCCAACAGGTCCCGTTAATACAATACTGTCAGCAACTTACCTCCACTCTTTCAAGTCATGATACCTGTTTCACTCAAGCAGCAGATTCACTATTTTTCATGCATGATGGGCTGCAGCAAGCTTGTGCTCCTGTTTATGATGTTCCATCTGCAATTGAAATCCTCCTAACAGGGTCATATCAGCGTTTGCCGAAATGTGTAGAAGACGTGGGCATCCAGAGCTCACTAAATGAGGAACAGCAGAAGCCGGCTTTGAAAAAGCTGGACACACTTGTGCGATCTAAATTACTGGAAGTATCACTTCCAAAAGAAATTACTGATGTTAAAGTCTCCGATGGTACAGCAGTGCTTCGTGTTGATGGAGAATTCAAGGTTTTGGTGACTCTAGGCTACCGAGGGCACCTGTCAATGTGGAGGATACTGCATTTGGAACTGCTTGTTGGTGAGAGAAGTGGACCTGTAAAGCTTGAAGTATCGCGGCGGCATCTTCTTGGGGATGATTTAGAGCGCAGAATGGCTGCTACAGAAAATCCGTTCATGATATTATACTCAGTTCTACATGAGCTTTGTGTTGCACTTATCATGGGCACTGTCACAAGGCAAGTACAAGCTCTTCGGCAAGGGAGATGGAAAGATGCAATTCGATTTGAGCTCATATCCGATGGGAGCATGAGTCATGCAGGGACATCTGCCTCTGCGCAATTAAATCAAGATGGAGAAACTGATTCATCTGGTCTACGAACGCCAGGGTTAAAAATTTTATACTGGTTAGATTTTGATAAAAACAATGGTATTTCTGATTCAGCATCATGTCCGTCTATAAAAATTGAACCAGGACCGGATCTGCAGATAAAGTGTCTTCATAGCACATTTGTTATAGATCCATTAACTGGAAAGGAGGCAGAATTTTCTCTTGACCAAAACTGTATTGATGTTGAGAAGTTGCTTCTGAGAGCCATCTGCTGTAACAGATATACTCGTCTGCTTGAAATTCAGAAAGAGCTGGTGAAAAATGTTCAAATCTGGCGAGGTGCAGGGGATGTTTCTCTTCAGTCTCATGTGGAAGAAGCGGATGTTGACCATAAAAAGAAAGAAGATAAGTCCCATGCTGGAGAATATGAAGGGCGGGAAGTATTATGTGTGCGTGCCTATGGCTCGTCATTTTTCACCCTGGGAATAAACATTAGGAATGGGCGATTTCGTCTTCAGTCCTCTCGTAATATTCTTGCTCCTTCGGGAGTTTTGTCTGAATGTGAAGATGCTTTAAATCAAGGAAGTATGACTGCAGCTGAAGTTTTCATAAATTTGAGAAGCAGAAGTATTTTGCACTTATTTGCATCTACTGGCCGATTTCTAGGGCTTGAGGTGTATGAACATAGTTTTCCTGCAGTTAAAATACCGAAGAACATTTTGAATGGTTCAACTATGTTGCTAATGGGGTTTCCTGATTGCGGGAGCTCTTATTTCTTGCTGATGCAACTTGATAAGGACTTTAAACCCCTATTTAAATTGCTTGAAACTCAGCCAGATCCGTCTAGAAAACCCGATTCTTTAAATGACTTAAACCAAGTGATGCGGATCAAGAAAATTGATGTTAGCCAGATGCAGATGCATGAAGATGATATGAATTTAAGCTTGCTTGACTTGGGAAAACTACAATCCTTTCTGCCCAGTTCCAGGGGTTCTAATCAGTCTTCTGAAAATGGTCTTCTTTCGGATATCAGCCATGAAGGTTCTATGCCTATTACAGGGTGTCCACCTTCGAGTTTTTCTTCTGTTGTAGATGAAGTGTTTGAACTAGAGAAGGGGTTATCTGTGCTTCCTTTTTCTGTTCCTGGTTCTCATTTTGGATCTGCTCCGATGAATCGTCCCTCTCCTAAATGGGAAGGAGTTATGCAGATATCACAGTTAAATAATTCTTCAAATCTTTCTAGCATGGCCACCCACTATAATGCATCCTTATATCCATCAAATAATTTGAAGGGCCCCGTACATTCTGCTTCTCTTGGTAATCTACCTTCTGGCCCAGGAAGGAGTGCCACTGTGAGGAAAATACCAGTGTCAAAATCTGATCAGGATTTGGCTTCTCTTAGGTCTCCACAGTCAGTTGAGTATGGTTCTGGTACTTCAATGGATGAAGATCAGCTGAGATTTATGAATGAAACTTCAAAGGGTGCAATATATGGGAATAAATCATCCCGGTTATTATCTCCACCTCGGTCTACTGGTCCCAGAATTTCTGGGCCTAGTGTGAGACCCAACGGGCCCAAAAGTACACCTAATGGGCCTTTAACTGGACCTTCCAGAGTTGCCGGATCAAACTCATGTGCAACCACTCCCGTATCCCAGGCCCCAGATTCGGGAGTATGTCATAGCCCTAACCATAATGTTTCAAAAAATGATATAAAACCTCGCAAACGCACAGTTTCAGATATGTTGAATTTGATCCCATCACTTCAGGGTGTGGAAGCCGATTCAGGAGTTTTTAAAAGGAGGAAAACTTCTGAAGTAACTCGTCCTCATCAGTCTTCTTCACAGATGCTTATGTCAAGGGATATAATTTCAAAATTTGAAGTATACAGTTACGGGGATCTGATGTCTGAAGCAAATAAGGGAAATGCACCTTCCAGCATTTATGTTTCAGCTCTTCTTCATGTGATCAGACACTGTTCTCTTTGTATTAAGCATGCCAGATTAACTAGCCAGATGGCGGCTCTGGACATCCCTTATGTTGAAGAAGTGGGTCTAAGAAGTACATCATCAAACATATGGTTTCGACTTCCATTTGCCAGAGGTGATGCATGGCAACACTTATGCTTGCGACTTGGCAGACCTGGAAGCATTTATTGGGATGTCAAAATAAATGACCAGCATTTCAGGGATTTGTGGGAACTTCAGAAAGGAAGCAATAGTACACCTTGGGGTTCTGGTGTTCGAATTGCCAATACATCTGACATTGACTCTCATATTTGCTATGATCCAGAGGGTGTTGTTCTTAGTTATCAGTCTGTGGAGGCTGATAGTATTAAGAAGTTAGTGGCAGACATCCAAAGACTTTCTAATGCAAGAATGTTTGCCCTTGGGATGCGGAGATTGCTTGGTGTAAGAGCAGATGAGAAGCCAGAAGAAAGTAGTACAAGCCCTGATTTTAAAGCACCAGGAGTCAAAGTTTCTCCTGAGGCAACTGATAGACTATCTGAGCAAATGCGAAGGGCATTTAGAATTGAGGCGGACCTTGTTGGACTTATGAGTCTGTGGTTCAGTTTTGGATCAGGTGTCCTTGCTCGCTTTGTTGTAGAGTGGGAATCAAGTAAAGAGGGTTGTACTATGCATGTAACCCCCGACCAACTTTGGCCCCATACCAAGTTCCTGGAAGATTTCATAAATGGAGCTGAAGTTGCTTCACTTTTGGATTGCATTCGACTCACTGCAGGGCCCTTGCATGCTCTTGCAGCTGCAACACGACCTGCACGAGCTAGTCCTATCCCGGGGGTCCCTGGAGGAGCAGTCCTCTCTTCTATCCCAAAGCAGGCTGGGTACTTACCATCTCAGGGCTTTATGCCTACTAGTTCAACCACAAATGCCGGACAGTCTCCTGGCCCTATGGGGAACCCAGTTTCATCTCCTTCTACAGGGCCTCTTGCAAATCATAGCCTGCATGGGGCTGCTGGGTTAGCTGGTGCTGGCCGAGGTGGACCTGGCATTGTACCCAGCTCACTGTTGCCCATTGATGTCTCTGTTGTGCTACGTGGACCCTATTGGATACGGATCATATACCGAAAGCACTTCGCAGTTGACATGCGCTGTTTTGCAGGAGATCAGGTTTGGTTGCAACCAGCAACCCCACCCAAGGGGGGCCCTTCCATTGGTGGGTCACTACCTTGCCCTCAGTTTAGACCATTTATTATGGAGCATGTAGCCCAAGAATTGAATGGTTTAGATACTAATTTCACCGGTGGTCAACAGACAGGACTGTCAAGTTCAATCAATCAAACGCCCAGTTCAGGCTCCCAACTGTCTACTGTAAATGGAAATAGGGTTAACCTCCCTGGTTCTGCTGCTATGTCCAGGACAGGCAACAACCAAGTAGCTGGTTTAAACCGTGTCGGAAATGCTTCTCCGGTATCCTCAAATTTGGCTGTTGTGAGCTCAGCAGGGCCGGCTTTACGTAGATCCCCAGGTCCTGGTGTCCCTGCTCATGTGAGAGGAGAACTGAATACAGCCATTATTGGTCTTGGAGATGACGGAGGGTATGGAGGTGGTTGGGTTCCTCTTGTTGCTCTTAAAAAGGTGCTAAGAGGTATTCTCAAGTACCTTGGAGTTCTATGGCTATTTGCCCAGCTACCAGGGCTCTTGAAAGAGATTTTGGGGTCAATTCTGAAAGACAATGAAGGCACACTTCTAAATTTAGACCAGGAGCAGCCTGCATTGAGATTCTTTGTTGGTGGCTATGTATTTGCAGTAAGTGTTCACAGAGTTCAACTTCTTCTTCAAGTACTGAGCGTGAAACGATTCCATCAGCAGCAGCAAGGGCAACAGCCAAATATAAACACTGCCAATGAGGAATTATCTACAGCCGAAATAGGAGAAATATGTGACTATTTCAGTCGGCGTGTTGCTTCAGAGCCTTATGATGCATCTCGTGTCGCATCATTCATTACTCTCCTTACCTTACCCATATCAGTTCTAAGAGAATTCTTGAAATTAATAGTATGGAAAAAAGGACTAGCCCAGGCACAAGGGGGAGATGTAGCACCTGCTCAAAAACCCCGTATTGAATTATGCCTTGAAAACCACTCTGGCTCGAGTATGGATGGGAGCTCAGATAACTCATCTGTTGCCAAAAGTAATATTTATTATAATCGGCCTCATAACTCGGTTGATTTTGCACTGACACTTGTTCTTGATCCTGCTCACATACCTCATATAAACGCCGCTGGTGGAGCTGCATGGTTGCCATACTGTGTCTCAGTGAGGTTGAGGTATTCATTTGGCGAAAATCCTAACGTGTCCTTTCTTGGTATGGAAGGAAGCCATGGAGGTCGAGCTTGCTGGTTGCGCGTTGATGACTGGGAGAAGTGTAAAAACAAGGTTGCTCGAACTGTGGAACATCAGGGTAGTTCGGGAGTAGACAGTAGTCAAGGAAGGTTAAGAATCGTAGCTGACTACGTGCAAAGGACACTGCATATGTGCTTCAAGGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005911 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008284 GO:0009266 GO:0009409 GO:0009506 GO:0009605 GO:0009607 GO:0009627 GO:0009628 GO:0009631 GO:0009814 GO:0016591 GO:0016592 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032991 GO:0042127 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045087 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0055029 GO:0055044 GO:0061695 GO:0065007 GO:0070013 GO:0070847 GO:0090575 GO:0098542 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04919 map04919

Pfam Domains

Protein Families

Med14

Protein Analysis

1804

Amino Acids

196.37

Weight (kDa)

8.35

Isoelectric Point (pI)

46.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med14	PF08638	9 - 199	1.9e-50	Mediator complex subunit MED14

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0009239)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04740
fragaria_vesca	FvH4_6g02880 FvH4_6g02880 FvH4_6g02880 FvH4_6g02880
malus_domestica	MD04G1224900.v1.1 MD12G1241600.v1.1
prunus_persica	Prupe.6G343600_v2.0.a1
pyrus_communis	pycom04g19810 pycom12g22110
rosa_chinensis	RchiOBHm_Chr3g0451091
rosa_laevigata	RLG00000025691
rosa_multiflora	Rmu_co8423931.1_g000001 Rmu_ssc0000461.1_g000005
rosa_roxburghii	Rroxscaffold_6G00428560
rosa_rugosa	Rorug02G0631200 Rorug02G0631300
rosa_samantha	Rh3AG034200 Rh3BG034500 Rh3CG033200 Rh3DG034200
rosa_wichuraiana	Rw3G002480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	5067
AarI	CACCTGC	1 cut(s)	4972
AasI	GACNNNNNNGTC	2 cut(s)	3456, 4180
Acc16I	TGCGCA	1 cut(s)	906
Acc36I	ACCTGC	2 cut(s)	3682, 4972
AccB1I	GGYRCC	2 cut(s)	207, 606
AccB7I	CCANNNNNTGG	2 cut(s)	1097, 4391
AccI	GTMKAC	5 cut(s)	943, 2429, 2784, 4226, 5337
AccII	CGCG	4 cut(s)	54, 77, 685, 5268
AcoI	YGGCCR	3 cut(s)	1552, 2145, 3892
AcuI	CTGAAG	9 cut(s)	1241, 1397, 1518, 2648, 2733, 2829, 3086, 3621, 4812
AcyI	GRCGYC	2 cut(s)	5, 107
AflIII	ACRYGT	1 cut(s)	5214
AgeI	ACCGGT	1 cut(s)	4160
AhlI	ACTAGT	1 cut(s)	3773
AjiI	CACGTC	1 cut(s)	415
AjuI	GAANNNNNNNTTGG	6 cut(s)	630, 662, 3037, 3069, 4556, 4588
AleI	CACNNNNGTG	1 cut(s)	479
Alw21I	GWGCWC	7 cut(s)	90, 337, 431, 861, 1667, 4358, 5030
Alw26I	GTCTC	7 cut(s)	541, 1266, 2307, 2455, 3804, 3946, 5179
AlwNI	CAGNNNCTG	2 cut(s)	2874, 4391
Ama87I	CYCGRG	2 cut(s)	3694, 5011
ApaI	GGGCCC	4 cut(s)	2195, 2475, 3645, 4067
AseI	ATTAAT	1 cut(s)	4913
AsiGI	ACCGGT	1 cut(s)	4160
Asp700I	GAANNNNTTC	2 cut(s)	4321, 4718
AspLEI	GCGC	6 cut(s)	56, 79, 717, 907, 4011, 5268
AsuC2I	CCSGG	3 cut(s)	2407, 3695, 3696
AsuHPI	GGTGA	7 cut(s)	58, 598, 662, 1225, 1366, 3014, 4150
AsuII	TTCGAA	1 cut(s)	3142
AvaI	CYCGRG	2 cut(s)	3694, 5011
AxyI	CCTNAGG	1 cut(s)	3384
BaeGI	GKGCMC	6 cut(s)	609, 797, 2195, 2475, 3645, 4067
BaeI	ACNNNNGTAYC	4 cut(s)	537, 570, 2532, 2565
BalI	TGGCCA	1 cut(s)	2147
BanI	GGYRCC	2 cut(s)	207, 606
BarI	GAAGNNNNNNTAC	6 cut(s)	804, 836, 2209, 2241, 3103, 3135
BauI	CACGAG	2 cut(s)	3678, 4848
BbsI	GAAGAC	6 cut(s)	417, 1058, 1403, 1893, 1907, 2727
Bbv12I	GWGCWC	7 cut(s)	90, 337, 431, 861, 1667, 4358, 5030
BciVI	GTATCC	4 cut(s)	618, 2560, 3963, 4342
BclI	TGATCA	2 cut(s)	2278, 2865
BcnI	CCSGG	3 cut(s)	2407, 3695, 3696
BcoDI	GTCTC	7 cut(s)	541, 1266, 2307, 2455, 3804, 3946, 5179
BcuI	ACTAGT	1 cut(s)	3773
BfmI	CTRYAG	8 cut(s)	320, 729, 1052, 1491, 1593, 3636, 3837, 4779
BfuAI	ACCTGC	2 cut(s)	3682, 4972
BfuI	GTATCC	4 cut(s)	618, 2560, 3963, 4342
BlpI	GCTNAGC	1 cut(s)	4357
BmcAI	AGTACT	2 cut(s)	155, 4704
BmeT110I	CYCGRG	2 cut(s)	3694, 5011
BmgBI	CACGTC	1 cut(s)	415
BmrI	ACTGGG	4 cut(s)	1872, 3815, 4200, 5287
BmuI	ACTGGG	4 cut(s)	1872, 3815, 4200, 5287
BoxI	GACNNNNGTC	1 cut(s)	5343
BpiI	GAAGAC	6 cut(s)	417, 1058, 1403, 1893, 1907, 2727
BplI	GAGNNNNNCTC	4 cut(s)	3702, 3734, 4497, 4529
BpmI	CTGGAG	2 cut(s)	1338, 3726
Bpu1102I	GCTNAGC	1 cut(s)	4357
Bpu14I	TTCGAA	1 cut(s)	3142
BpuEI	CTTGAG	4 cut(s)	134, 267, 1589, 4499
BpuMI	CCSGG	3 cut(s)	2407, 3695, 3696
BsaAI	YACGTR	3 cut(s)	3956, 4376, 5377
BsaBI	GATNNNNATC	3 cut(s)	1784, 2346, 3974
BsaHI	GRCGYC	2 cut(s)	5, 107
BsaI	GGTCTC	2 cut(s)	2307, 2455
BsaWI	WCCGGW	3 cut(s)	1045, 4160, 4327
Bse118I	RCCGGY	3 cut(s)	454, 4160, 4366
Bse21I	CCTNAGG	1 cut(s)	3384
Bse8I	GATNNNNATC	3 cut(s)	1784, 2346, 3974
BseJI	GATNNNNATC	3 cut(s)	1784, 2346, 3974
BseRI	GAGGAG	2 cut(s)	3723, 4428
BseSI	GKGCMC	6 cut(s)	609, 797, 2195, 2475, 3645, 4067
BseYI	CCCAGC	5 cut(s)	168, 3740, 3875, 3917, 4545
BsgI	GTGCAG	2 cut(s)	1260, 3660
Bsh1236I	CGCG	4 cut(s)	54, 77, 685, 5268
BshNI	GGYRCC	2 cut(s)	207, 606
BshTI	ACCGGT	1 cut(s)	4160
BsiHKAI	GWGCWC	7 cut(s)	90, 337, 431, 861, 1667, 4358, 5030
BsiHKCI	CYCGRG	2 cut(s)	3694, 5011
BslFI	GGGAC	7 cut(s)	203, 904, 1294, 2061, 2421, 3686, 4382
BsmAI	GTCTC	7 cut(s)	541, 1266, 2307, 2455, 3804, 3946, 5179
BsmFI	GGGAC	7 cut(s)	203, 904, 1294, 2061, 2421, 3686, 4382
BsmI	GAATGC	1 cut(s)	3624
Bso31I	GGTCTC	2 cut(s)	2307, 2455
BsoBI	CYCGRG	2 cut(s)	3694, 5011
Bsp119I	TTCGAA	1 cut(s)	3142
Bsp120I	GGGCCC	4 cut(s)	2191, 2471, 3641, 4063
Bsp1720I	GCTNAGC	1 cut(s)	4357
Bsp19I	CCATGG	1 cut(s)	5242
BspFNI	CGCG	4 cut(s)	54, 77, 685, 5268
BspHI	TCATGA	2 cut(s)	265, 744
BspMAI	CTGCAG	5 cut(s)	324, 1056, 1495, 1597, 3640
BspMI	ACCTGC	2 cut(s)	3682, 4972
BspQI	GCTCTTC	2 cut(s)	825, 2859
BspT104I	TTCGAA	1 cut(s)	3142
BspT107I	GGYRCC	2 cut(s)	207, 606
BspTNI	GGTCTC	2 cut(s)	2307, 2455
BsrFI	RCCGGY	3 cut(s)	454, 4160, 4366
BssAI	RCCGGY	3 cut(s)	454, 4160, 4366
BssNAI	GTATAC	1 cut(s)	2785
BssNI	GRCGYC	2 cut(s)	5, 107
BssSI	CACGAG	2 cut(s)	3678, 4848
BssT1I	CCWWGG	5 cut(s)	3125, 3286, 4056, 4522, 5242
Bst1107I	GTATAC	1 cut(s)	2785
Bst2BI	CACGAG	2 cut(s)	3678, 4848
Bst6I	CTCTTC	4 cut(s)	825, 1260, 2859, 3727
BstACI	GRCGYC	2 cut(s)	5, 107
BstAPI	GCANNNNNTGC	1 cut(s)	178
BstBAI	YACGTR	3 cut(s)	3956, 4376, 5377
BstBI	TTCGAA	1 cut(s)	3142
BstDSI	CCRYGG	1 cut(s)	5242
BstENI	CCTNNNNNAGG	2 cut(s)	1953, 3837
BstFNI	CGCG	4 cut(s)	54, 77, 685, 5268
BstHHI	GCGC	6 cut(s)	56, 79, 717, 907, 4011, 5268
BstMAI	GTCTC	7 cut(s)	541, 1266, 2307, 2455, 3804, 3946, 5179
BstNSI	RCATGY	6 cut(s)	181, 2897, 3542, 3653, 4009, 4124
BstPAI	GACNNNNGTC	1 cut(s)	5343
BstSFI	CTRYAG	8 cut(s)	320, 729, 1052, 1491, 1593, 3636, 3837, 4779
BstSLI	GKGCMC	6 cut(s)	609, 797, 2195, 2475, 3645, 4067
BstSNI	TACGTA	1 cut(s)	4376
BstUI	CGCG	4 cut(s)	54, 77, 685, 5268
BstV2I	GAAGAC	6 cut(s)	417, 1058, 1403, 1893, 1907, 2727
BstX2I	RGATCY	6 cut(s)	1048, 1087, 1735, 2057, 2797, 4379
BstXI	CCANNNNNNTGG	2 cut(s)	3577, 4298
BstYI	RGATCY	6 cut(s)	1048, 1087, 1735, 2057, 2797, 4379
BstZ17I	GTATAC	1 cut(s)	2785
Bsu36I	CCTNAGG	1 cut(s)	3384
BsuI	GTATCC	4 cut(s)	618, 2560, 3963, 4342
BtgI	CCRYGG	1 cut(s)	5242
BtrI	CACGTC	1 cut(s)	415
BtsI	GCAGTG	4 cut(s)	558, 3633, 4758, 5387
BveI	ACCTGC	2 cut(s)	3682, 4972
CaiI	CAGNNNCTG	2 cut(s)	2874, 4391
CciI	TCATGA	2 cut(s)	265, 744
CfoI	GCGC	6 cut(s)	56, 79, 717, 907, 4011, 5268
Cfr10I	RCCGGY	3 cut(s)	454, 4160, 4366
Cfr9I	CCCGGG	1 cut(s)	3694
CseI	GACGC	1 cut(s)	96
CspAI	ACCGGT	1 cut(s)	4160
DraI	TTTAAA	7 cut(s)	1473, 1702, 1714, 1761, 2695, 3358, 4305
DrdI	GACNNNNNNGTC	2 cut(s)	3456, 4180
DseDI	GACNNNNNNGTC	2 cut(s)	3456, 4180
EaeI	YGGCCR	3 cut(s)	1552, 2145, 3892
Eam1104I	CTCTTC	4 cut(s)	825, 1260, 2859, 3727
EarI	CTCTTC	4 cut(s)	825, 1260, 2859, 3727
EciI	GGCGGA	2 cut(s)	28, 3452
Ecl136II	GAGCTC	6 cut(s)	88, 429, 859, 1665, 4356, 5028
Eco105I	TACGTA	1 cut(s)	4376
Eco130I	CCWWGG	5 cut(s)	3125, 3286, 4056, 4522, 5242
Eco31I	GGTCTC	2 cut(s)	2307, 2455
Eco32I	GATATC	2 cut(s)	1927, 2109
Eco53kI	GAGCTC	6 cut(s)	88, 429, 859, 1665, 4356, 5028
Eco57I	CTGAAG	9 cut(s)	1241, 1397, 1518, 2648, 2733, 2829, 3086, 3621, 4812
Eco81I	CCTNAGG	1 cut(s)	3384
Eco88I	CYCGRG	2 cut(s)	3694, 5011
EcoICRI	GAGCTC	6 cut(s)	88, 429, 859, 1665, 4356, 5028
EcoNI	CCTNNNNNAGG	2 cut(s)	1953, 3837
EcoRI	GAATTC	2 cut(s)	572, 4901
EcoRV	GATATC	2 cut(s)	1927, 2109
EcoT14I	CCWWGG	5 cut(s)	3125, 3286, 4056, 4522, 5242
EcoT22I	ATGCAT	6 cut(s)	312, 1821, 2164, 3010, 3540, 4846
ErhI	CCWWGG	5 cut(s)	3125, 3286, 4056, 4522, 5242
FalI	AAGNNNNNCTT	4 cut(s)	492, 524, 4590, 4622
FaqI	GGGAC	7 cut(s)	203, 904, 1294, 2061, 2421, 3686, 4382
FauI	CCCGC	2 cut(s)	1326, 1652
FauNDI	CATATG	2 cut(s)	2975, 5394
FbaI	TGATCA	2 cut(s)	2278, 2865
FblI	GTMKAC	5 cut(s)	943, 2429, 2784, 4226, 5337
FspI	TGCGCA	1 cut(s)	906
GlaI	GCGC	6 cut(s)	55, 78, 716, 906, 4010, 5267
GsaI	CCCAGC	5 cut(s)	172, 3744, 3879, 3921, 4549
GsuI	CTGGAG	2 cut(s)	1338, 3726
HgaI	GACGC	1 cut(s)	96
HhaI	GCGC	6 cut(s)	56, 79, 717, 907, 4011, 5268
Hin1I	GRCGYC	2 cut(s)	5, 107
Hin6I	GCGC	6 cut(s)	54, 77, 715, 905, 4009, 5266
HinP1I	GCGC	6 cut(s)	54, 77, 715, 905, 4009, 5266
HincII	GTYRAC	4 cut(s)	1285, 4003, 4169, 4249
HindII	GTYRAC	4 cut(s)	1285, 4003, 4169, 4249
HindIII	AAGCTT	3 cut(s)	327, 671, 1840
HpaI	GTTAAC	1 cut(s)	4249
HphI	GGTGA	7 cut(s)	58, 598, 662, 1225, 1366, 3014, 4150
Hpy99I	CGWCG	2 cut(s)	10, 112
HpyCH4IV	ACGT	5 cut(s)	414, 3955, 4375, 5214, 5376
HpySE526I	ACGT	5 cut(s)	414, 3955, 4375, 5214, 5376
Hsp92I	GRCGYC	2 cut(s)	5, 107
HspAI	GCGC	6 cut(s)	54, 77, 715, 905, 4009, 5266
KflI	GGGWCCC	1 cut(s)	3700
KroI	GCCGGC	2 cut(s)	454, 4366
KroNI	GCCGGC	2 cut(s)	456, 4368
Ksp22I	TGATCA	2 cut(s)	2278, 2865
KspAI	GTTAAC	1 cut(s)	4249
LguI	GCTCTTC	2 cut(s)	825, 2859
MaeII	ACGT	5 cut(s)	414, 3955, 4375, 5214, 5376
MaeIII	GTNAC	8 cut(s)	586, 799, 1172, 2716, 2789, 3541, 4080, 4802
MfeI	CAATTG	1 cut(s)	360
MflI	RGATCY	6 cut(s)	1048, 1087, 1735, 2057, 2797, 4379
MlsI	TGGCCA	1 cut(s)	2147
MluNI	TGGCCA	1 cut(s)	2147
MlyI	GAGTC	6 cut(s)	583, 889, 3161, 3378, 3466, 3624
MmeI	TCCRAC	2 cut(s)	3427, 4294
Mox20I	TGGCCA	1 cut(s)	2147
Mph1103I	ATGCAT	6 cut(s)	312, 1821, 2164, 3010, 3540, 4846
MroNI	GCCGGC	2 cut(s)	454, 4366
MroXI	GAANNNNTTC	2 cut(s)	4321, 4718
MscI	TGGCCA	1 cut(s)	2147
MslI	CAYNNNNRTG	3 cut(s)	479, 1824, 2157
Msp20I	TGGCCA	1 cut(s)	2147
MspA1I	CMGCKG	4 cut(s)	1496, 2353, 3662, 5144
MssI	GTTTAAAC	1 cut(s)	4305
MunI	CAATTG	1 cut(s)	360
Mva1269I	GAATGC	1 cut(s)	3624
MvnI	CGCG	4 cut(s)	54, 77, 685, 5268
NaeI	GCCGGC	2 cut(s)	456, 4368
NciI	CCSGG	3 cut(s)	2407, 3695, 3696
NcoI	CCATGG	1 cut(s)	5242
NdeI	CATATG	2 cut(s)	2975, 5394
NgoMIV	GCCGGC	2 cut(s)	454, 4366
NmeAIII	GCCGAG	2 cut(s)	85, 3920
NmuCI	GTSAC	4 cut(s)	586, 799, 4080, 4802
NsbI	TGCGCA	1 cut(s)	906
NsiI	ATGCAT	6 cut(s)	312, 1821, 2164, 3010, 3540, 4846
NspI	RCATGY	6 cut(s)	181, 2897, 3542, 3653, 4009, 4124
NspV	TTCGAA	1 cut(s)	3142
OliI	CACNNNNGTG	1 cut(s)	479
PaeI	GCATGC	3 cut(s)	181, 2897, 3653
PaeR7I	CTCGAG	1 cut(s)	5011
PagI	TCATGA	2 cut(s)	265, 744
PaqCI	CACCTGC	1 cut(s)	4972
PasI	CCCWGGG	1 cut(s)	1378
PciSI	GCTCTTC	2 cut(s)	825, 2859
PcsI	WCGNNNNNNNCGW	1 cut(s)	3676
PctI	GAATGC	1 cut(s)	3624
PdiI	GCCGGC	2 cut(s)	456, 4368
PdmI	GAANNNNTTC	2 cut(s)	4321, 4718
PflMI	CCANNNNNTGG	2 cut(s)	1097, 4391
PfoI	TCCNGGA	2 cut(s)	3576, 4274
PinAI	ACCGGT	1 cut(s)	4160
PleI	GAGTC	6 cut(s)	583, 888, 3161, 3377, 3465, 3624
PmeI	GTTTAAAC	1 cut(s)	4305
PpsI	GAGTC	6 cut(s)	583, 888, 3161, 3377, 3465, 3624
Ppu21I	YACGTR	3 cut(s)	3956, 4376, 5377
PpuMI	RGGWCCY	3 cut(s)	217, 3700, 4388
PshAI	GACNNNNGTC	1 cut(s)	5343
PshBI	ATTAAT	1 cut(s)	4913
PsiI	TTATAA	1 cut(s)	5067
Psp124BI	GAGCTC	6 cut(s)	90, 431, 861, 1667, 4358, 5030
Psp5II	RGGWCCY	3 cut(s)	217, 3700, 4388
PspFI	CCCAGC	5 cut(s)	168, 3740, 3875, 3917, 4545
PspOMI	GGGCCC	4 cut(s)	2191, 2471, 3641, 4063
PspPPI	RGGWCCY	3 cut(s)	217, 3700, 4388
PspXI	VCTCGAGB	1 cut(s)	5011
PstI	CTGCAG	5 cut(s)	324, 1056, 1495, 1597, 3640
PstNI	CAGNNNCTG	2 cut(s)	2874, 4391
PsuI	RGATCY	6 cut(s)	1048, 1087, 1735, 2057, 2797, 4379
PvuII	CAGCTG	3 cut(s)	1496, 2353, 3662
RseI	CAYNNNNRTG	3 cut(s)	479, 1824, 2157
SacI	GAGCTC	6 cut(s)	90, 431, 861, 1667, 4358, 5030
SapI	GCTCTTC	2 cut(s)	825, 2859
ScaI	AGTACT	2 cut(s)	155, 4704
SchI	GAGTC	6 cut(s)	583, 889, 3161, 3378, 3466, 3624
SfcI	CTRYAG	8 cut(s)	320, 729, 1052, 1491, 1593, 3636, 3837, 4779
Sfr274I	CTCGAG	1 cut(s)	5011
SfuI	TTCGAA	1 cut(s)	3142
SgrAI	CRCCGGYG	1 cut(s)	4160
SlaI	CTCGAG	1 cut(s)	5011
SmaI	CCCGGG	1 cut(s)	3696
SmiI	ATTTAAAT	1 cut(s)	1714
SmiMI	CAYNNNNRTG	3 cut(s)	479, 1824, 2157
SmlI	CTYRAG	5 cut(s)	149, 282, 1568, 4514, 5011
SmoI	CTYRAG	5 cut(s)	149, 282, 1568, 4514, 5011
SnaBI	TACGTA	1 cut(s)	4376
SpeI	ACTAGT	1 cut(s)	3773
SphI	GCATGC	3 cut(s)	181, 2897, 3653
SspI	AATATT	2 cut(s)	1429, 5059
SstI	GAGCTC	6 cut(s)	90, 431, 861, 1667, 4358, 5030
StyI	CCWWGG	5 cut(s)	3125, 3286, 4056, 4522, 5242
SwaI	ATTTAAAT	1 cut(s)	1714
TaiI	ACGT	5 cut(s)	417, 3958, 4378, 5217, 5379
TaqI	TCGA	9 cut(s)	101, 850, 1970, 2983, 3142, 3628, 5012, 5251, 5305
TaqII	GACCGA	1 cut(s)	2415
TatI	WGTACW	8 cut(s)	153, 811, 2480, 2960, 3119, 3341, 3530, 4702
TauI	GCSGC	6 cut(s)	54, 59, 688, 691, 2921, 5144
TseFI	GTSAC	4 cut(s)	586, 799, 4080, 4802
Tsp45I	GTSAC	4 cut(s)	586, 799, 4080, 4802
TspGWI	ACGGA	5 cut(s)	729, 1011, 1728, 3988, 4467
TspMI	CCCGGG	1 cut(s)	3694
Van91I	CCANNNNNTGG	2 cut(s)	1097, 4391
VspI	ATTAAT	1 cut(s)	4913
XagI	CCTNNNNNAGG	2 cut(s)	1953, 3837
XbaI	TCTAGA	1 cut(s)	1742
XceI	RCATGY	6 cut(s)	181, 2897, 3542, 3653, 4009, 4124
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4136
XhoI	CTCGAG	1 cut(s)	5011
XmaI	CCCGGG	1 cut(s)	3694
XmiI	GTMKAC	5 cut(s)	943, 2429, 2784, 4226, 5337
XmnI	GAANNNNTTC	2 cut(s)	4321, 4718
ZrmI	AGTACT	2 cut(s)	155, 4704
Zsp2I	ATGCAT	6 cut(s)	312, 1821, 2164, 3010, 3540, 4846

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.