pycom12g22510

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012738
Physical Location & Seq
Forward (+)
23381723 .. 23382987
1265 bp
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UTR
Exon/CDS
Intron
pycom12g22510.1

Sequence Viewer

Length: 612 bp
ATGTACCACCCAATATACCGCTCAACTTCCTCACTGTTCGTCTCCAATCCAAATCTCATCTTCAGATTGGAAGTTGTAGAGAAAATGTCGAACCCGAAAGTTTTCTTTGACATATTGATCGGAAGGATGAAGGCGGGTCGAGTTGTAATGGAGTTATTTGCTGATAAAACCCCGAAAACTGCTGAAAATTTCCGTGCTCTTTGCACCGGAGACAAGGGGATTGGATTATCAGGGAAGCCACTGCACTTTAAGGGATCCACATTTCATCGCATTATCCCGAACTTCATGTGCCAGGGCGGAGATTTCACAAGAGGCAACGGAACTGGGGGAGAGTCGATTTATGGGGCGAAATTCGCAGATGAGAACTTCAACTTGAAGCATACTGGCGCTGGAATTTTGTCCATGGCGAATTCTGGACCTAATACGAACGGTTCACAGTTCTTCATTTGCACCGCAAGCACACCTTGGCTTGACGGAAAGCACGTTGTGTTTGGAAAAGTGGTCGATGGATACAGTGTTGTTGTGAAGATGGAAAAGGTGGGTTCAGAGCGTGGGACTACTTCTGAACCTGTTGTGATCGAAGATTGCGGTCAGATAAAAGATGAGTGTTGA

Protein Analysis

204

Amino Acids

22.14

Weight (kDa)

8.57

Isoelectric Point (pI)

17.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_isomerase PF00160 42 - 197 2.5e-49 Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012738)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56070 AT3G56070
fragaria_vesca FvH4_4g00620 FvH4_4g00620 FvH4_6g02290
malus_domestica MD04G1228500.v1.1
prunus_persica Prupe.6G348500_v2.0.a1
pyrus_communis pycom04g20270 pycom12g22510
rosa_chinensis RchiOBHm_Chr3g0450041
rosa_laevigata RLG00000025762 RLG00000025763
rosa_multiflora Rmu_sc0002646.1_g000022
rosa_roxburghii Rroxscaffold_6G00427830
rosa_rugosa Rorug02G0625000
rosa_samantha Rh3BG024700 Rh3DG024700
rosa_wichuraiana Rw3G001920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 21
AciI CCGC 5 cut(s) 19, 134, 297, 453, 588
AclWI GGATC 2 cut(s) 249, 262
AcsI RAATTY 4 cut(s) 187, 350, 393, 409
AcuI CTGAAG 1 cut(s) 46
AdeI CACNNNGTG 1 cut(s) 487
AfaI GTAC 1 cut(s) 5
AgsI TTSAA 2 cut(s) 370, 376
AjnI CCWGG 1 cut(s) 291
Alw21I GWGCWC 1 cut(s) 199
Alw26I GTCTC 2 cut(s) 46, 204
AlwI GGATC 2 cut(s) 249, 262
ApoI RAATTY 4 cut(s) 187, 350, 393, 409
Asp700I GAANNNNTTC 1 cut(s) 101
AspLEI GCGC 1 cut(s) 389
AspS9I GGNCC 1 cut(s) 416
AvaII GGWCC 1 cut(s) 416
BamHI GGATCC 1 cut(s) 254
Bbv12I GWGCWC 1 cut(s) 199
BccI CCATC 2 cut(s) 500, 523
BciT130I CCWGG 1 cut(s) 293
BciVI GTATCC 1 cut(s) 503
BcoDI GTCTC 2 cut(s) 46, 204
BfoI RGCGCY 1 cut(s) 390
BfuI GTATCC 1 cut(s) 503
Bme1390I CCNGG 1 cut(s) 293
Bme18I GGWCC 1 cut(s) 416
BmgT120I GGNCC 1 cut(s) 416
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 1 cut(s) 293
BmrI ACTGGG 1 cut(s) 333
BmuI ACTGGG 1 cut(s) 333
BsaJI CCNNGG 3 cut(s) 292, 402, 464
BsaWI WCCGGW 1 cut(s) 206
Bse1I ACTGG 2 cut(s) 328, 388
BseBI CCWGG 1 cut(s) 293
BseDI CCNNGG 3 cut(s) 292, 402, 464
BseGI GGATG 1 cut(s) 132
BseNI ACTGG 2 cut(s) 328, 388
BsgI GTGCAG 1 cut(s) 227
BsiHKAI GWGCWC 1 cut(s) 199
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 1 cut(s) 568
BsmAI GTCTC 2 cut(s) 46, 204
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 568
Bsp1286I GDGCHC 1 cut(s) 199
Bsp143I GATC 3 cut(s) 117, 254, 576
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 5 cut(s) 19, 134, 297, 453, 588
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 2 cut(s) 249, 262
BsrBI CCGCTC 1 cut(s) 21
BsrI ACTGG 2 cut(s) 328, 388
BssECI CCNNGG 3 cut(s) 292, 402, 464
BssMI GATC 3 cut(s) 117, 254, 576
BssT1I CCWWGG 2 cut(s) 402, 464
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 4 cut(s) 36, 431, 438, 515
BstC8I GCNNGC 1 cut(s) 457
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 1 cut(s) 132
BstH2I RGCGCY 1 cut(s) 390
BstHHI GCGC 1 cut(s) 389
BstKTI GATC 3 cut(s) 120, 257, 579
BstMAI GTCTC 2 cut(s) 46, 204
BstMBI GATC 3 cut(s) 117, 254, 576
BstMWI GCNNNNNNNGC 2 cut(s) 353, 456
BstNI CCWGG 1 cut(s) 293
BstSCI CCNGG 1 cut(s) 291
BstX2I RGATCY 1 cut(s) 254
BstYI RGATCY 1 cut(s) 254
BsuI GTATCC 1 cut(s) 503
BtgI CCRYGG 1 cut(s) 402
BtgZI GCGATG 1 cut(s) 251
BtsCI GGATG 1 cut(s) 132
BtsI GCAGTG 1 cut(s) 239
BtsIMutI CAGTG 3 cut(s) 32, 239, 520
Cac8I GCNNGC 1 cut(s) 457
CfoI GCGC 1 cut(s) 389
Cfr13I GGNCC 1 cut(s) 416
Csp6I GTAC 1 cut(s) 4
CviAII CATG 2 cut(s) 286, 403
CviJI RGCY 2 cut(s) 238, 469
CviKI_1 RGCY 2 cut(s) 238, 469
CviQI GTAC 1 cut(s) 4
DpnI GATC 3 cut(s) 119, 256, 578
DpnII GATC 3 cut(s) 117, 254, 576
DraIII CACNNNGTG 1 cut(s) 487
EciI GGCGGA 1 cut(s) 312
Eco130I CCWWGG 2 cut(s) 402, 464
Eco47I GGWCC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 46
EcoRI GAATTC 1 cut(s) 409
EcoRII CCWGG 1 cut(s) 291
EcoT14I CCWWGG 2 cut(s) 402, 464
ErhI CCWWGG 2 cut(s) 402, 464
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 2 cut(s) 289, 406
FaiI YATR 6 cut(s) 16, 113, 287, 342, 381, 404
FalI AAGNNNNNCTT 2 cut(s) 448, 480
FaqI GGGAC 1 cut(s) 568
FatI CATG 2 cut(s) 285, 402
FauI CCCGC 1 cut(s) 127
FokI GGATG 1 cut(s) 139
GlaI GCGC 1 cut(s) 388
HaeII RGCGCY 1 cut(s) 390
HapII CCGG 1 cut(s) 207
HhaI GCGC 1 cut(s) 389
Hin1II CATG 2 cut(s) 289, 406
Hin6I GCGC 1 cut(s) 387
HinP1I GCGC 1 cut(s) 387
HinfI GANTC 1 cut(s) 332
HpaII CCGG 1 cut(s) 207
Hpy166II GTNNAC 1 cut(s) 434
Hpy188I TCNGA 5 cut(s) 65, 122, 547, 565, 594
Hpy188III TCNNGA 2 cut(s) 277, 414
Hpy8I GTNNAC 1 cut(s) 434
HpyAV CCTTC 2 cut(s) 117, 124
HpyCH4III ACNGT 4 cut(s) 36, 431, 438, 515
HpyCH4IV ACGT 1 cut(s) 483
HpyCH4V TGCA 3 cut(s) 204, 244, 450
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 456
HpySE526I ACGT 1 cut(s) 483
Hsp92II CATG 2 cut(s) 289, 406
HspAI GCGC 1 cut(s) 387
Kzo9I GATC 3 cut(s) 117, 254, 576
LpnPI CCDG 9 cut(s) 216, 220, 278, 305, 309, 369, 375, 399, 582
MaeII ACGT 1 cut(s) 483
MalI GATC 3 cut(s) 119, 256, 578
MbiI CCGCTC 1 cut(s) 21
MboI GATC 3 cut(s) 117, 254, 576
MboII GAAGA 4 cut(s) 52, 433, 538, 593
MflI RGATCY 1 cut(s) 254
MhlI GDGCHC 1 cut(s) 199
MluCI AATT 4 cut(s) 187, 350, 393, 409
MlyI GAGTC 1 cut(s) 341
MnlI CCTC 2 cut(s) 40, 305
MroXI GAANNNNTTC 1 cut(s) 101
MseI TTAA 1 cut(s) 249
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 293
MvaI CCWGG 1 cut(s) 293
MwoI GCNNNNNNNGC 2 cut(s) 353, 456
NcoI CCATGG 1 cut(s) 402
NdeII GATC 3 cut(s) 117, 254, 576
NlaIII CATG 2 cut(s) 289, 406
NlaIV GGNNCC 1 cut(s) 256
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PdmI GAANNNNTTC 1 cut(s) 101
PleI GAGTC 1 cut(s) 340
PpsI GAGTC 1 cut(s) 340
Psp6I CCWGG 1 cut(s) 291
PspGI CCWGG 1 cut(s) 291
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 1 cut(s) 416
PsuI RGATCY 1 cut(s) 254
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 249
Sau3AI GATC 3 cut(s) 117, 254, 576
Sau96I GGNCC 1 cut(s) 416
SchI GAGTC 1 cut(s) 341
ScrFI CCNGG 1 cut(s) 293
SduI GDGCHC 1 cut(s) 199
SetI ASST 5 cut(s) 421, 466, 486, 540, 571
SinI GGWCC 1 cut(s) 416
Sse9I AATT 4 cut(s) 187, 350, 393, 409
SsiI CCGC 5 cut(s) 19, 134, 297, 453, 588
StyD4I CCNGG 1 cut(s) 291
StyI CCWWGG 2 cut(s) 402, 464
TaaI ACNGT 4 cut(s) 36, 431, 438, 515
TaiI ACGT 1 cut(s) 486
TaqI TCGA 5 cut(s) 89, 139, 335, 504, 579
TasI AATT 4 cut(s) 187, 350, 393, 409
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 3 cut(s) 39, 246, 520
TspDTI ATGAA 4 cut(s) 143, 254, 274, 433
TspGWI ACGGA 3 cut(s) 182, 333, 489
TspRI CASTG 3 cut(s) 39, 246, 520
VpaK11BI GGWCC 1 cut(s) 416
XapI RAATTY 4 cut(s) 187, 350, 393, 409
XmnI GAANNNNTTC 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.