pycom12g23770

trigalactosyldiacylglycerol 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014402
Physical Location & Seq
Reverse (-)
24255207 .. 24258139
2933 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1059 bp
ATGGTTTCTCTGTCGAATTCAGTGTTGTTTCCTCTGGCGACATCCAGTGGCTTCACATCCACTGGTTTTTCTCGGCCCGTTCGATTTGCAAGGACGATCAATTGGTATAGTAGTGACAAGCAAAGGGAAGAGCCCAGAAACGTTTTGTGTGCTTGTATGGCCCCTTCCCCGAACCTGCGGAGCGATGAATCCGTTGCTACAAAATTTGAGGATTCATTTAAATCAGAGGATTTAAGTAGAGTTCGGGAGCCTGAGGGTGATTCTGATGTTCTGATCGAGTGTAGAGACGTCTACAAATCGTTTGGCGAGAAGCATATATTGAGAGGTGTGAGCTTCAAGATTAGGCATGGTGAAGCTGTTGGAATAATTGGGCCTTCTGGCACCGGAAAATCTACAGTTCTGAAGATAATGGCAGGGCTTCTTGCTCCTGACAAGGGAGAGGTGTACATTCGAGGTCGAAAGAGAGTTGGTTTGATCAGCGATGATGAGATATCCGGTCTTCGGATTGGATTGGTGTTTCAGAGCGCTGCACTTTTTGATTCTTTGACTGTTCGTGAAAATGTCGGTTTTCTTCTGTATGAAAATTCGAGCATGCCCGAGGATCAAATTGCAAAGCTTGTGACTGAGAGCTTGGCTGCAGTCGGGTTAAAGGGAGTTGAAGATCGGTTACCTTCGGAGCTGTCTGGTGGAATGAAGAAACGAGTTGCTCTGGCTCGATCCATAATTTATGATACCACAAAGGAAGCGATAGAGCCAGAGGTGCTTTTATATGATGAGCCAACAGCTGGACTTGATCCTATTGCATCTACTGTGGTAGAAGACCTCATCCGCTCTGTTCATACAAAAGGTCAGGATGCAGTCGAGAAACCTGGGCAGATCGCATCTTATGTTGTTGTTACTCACCAACATAGTACCATCAGACGAGCTGTTGACAGGTTGTTGTTTCTCTACGAGGGCGAGATAGTCTGGCAAGGAATGACTCATGAATTCACCACCTCAACAAATCCAATCGTTCAACAGTTTGCATCCGGGAACTTGGACGGCCCGATTAAATATTAG

Protein Analysis

353

Amino Acids

38.65

Weight (kDa)

5.67

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 107 - 262 7e-30 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014402)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65410
fragaria_vesca FvH4_6g00670 FvH4_6g00670
malus_domestica MD04G1243700.v1.1 MD12G1259300.v1.1
prunus_persica Prupe.6G359900_v2.0.a1
pyrus_communis pycom04g21440 pycom12g23770
rosa_chinensis RchiOBHm_Chr3g0448111
rosa_laevigata RLG00000025946
rosa_multiflora Rmu_sc0005969.1_g000027
rosa_roxburghii Rroxscaffold_6G00426240
rosa_rugosa Rorug02G0608200
rosa_samantha Rh3AG007700 Rh3CG007400 Rh3DG007900
rosa_wichuraiana Rw3G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 291
Acc36I ACCTGC 1 cut(s) 183
AccB1I GGYRCC 1 cut(s) 380
AccB7I CCANNNNNTGG 1 cut(s) 785
AccBSI CCGCTC 1 cut(s) 831
AccI GTMKAC 1 cut(s) 291
AciI CCGC 2 cut(s) 178, 829
AclI AACGTT 1 cut(s) 141
AclWI GGATC 3 cut(s) 609, 711, 788
AcsI RAATTY 4 cut(s) 16, 203, 583, 986
AcuI CTGAAG 1 cut(s) 422
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 2 cut(s) 446, 913
AfeI AGCGCT 1 cut(s) 526
AfiI CCNNNNNNNGG 3 cut(s) 434, 501, 785
AgsI TTSAA 3 cut(s) 337, 659, 1016
AjnI CCWGG 1 cut(s) 868
AluBI AGCT 7 cut(s) 333, 356, 616, 630, 679, 785, 926
AluI AGCT 7 cut(s) 333, 356, 616, 630, 679, 785, 926
Alw26I GTCTC 1 cut(s) 279
AlwI GGATC 3 cut(s) 609, 711, 788
Ama87I CYCGRG 1 cut(s) 596
Aor51HI AGCGCT 1 cut(s) 526
AoxI GGCC 4 cut(s) 74, 159, 371, 1042
ApeKI GCWGC 2 cut(s) 527, 635
ApoI RAATTY 4 cut(s) 16, 203, 583, 986
AspLEI GCGC 1 cut(s) 527
AspS9I GGNCC 4 cut(s) 75, 160, 371, 1043
AsuC2I CCSGG 1 cut(s) 1030
AsuHPI GGTGA 4 cut(s) 269, 362, 893, 982
AvaI CYCGRG 1 cut(s) 596
AxyI CCTNAGG 1 cut(s) 252
BanI GGYRCC 1 cut(s) 380
BanII GRGCYC 1 cut(s) 135
BarI GAAGNNNNNNTAC 4 cut(s) 148, 180, 651, 683
BbsI GAAGAC 2 cut(s) 491, 825
BbvI GCAGC 2 cut(s) 514, 622
BccI CCATC 1 cut(s) 923
BceAI ACGGC 1 cut(s) 1057
BciT130I CCWGG 1 cut(s) 870
BclI TGATCA 1 cut(s) 474
BcnI CCSGG 1 cut(s) 1030
BcoDI GTCTC 1 cut(s) 279
BfmI CTRYAG 2 cut(s) 393, 636
BfoI RGCGCY 1 cut(s) 528
BfuAI ACCTGC 1 cut(s) 183
BisI GCNGC 2 cut(s) 528, 636
BlsI GCNGC 2 cut(s) 529, 637
Bme1390I CCNGG 2 cut(s) 870, 1030
BmeT110I CYCGRG 1 cut(s) 596
BmgT120I GGNCC 4 cut(s) 75, 160, 371, 1043
BmiI GGNNCC 3 cut(s) 162, 249, 382
BmrFI CCNGG 2 cut(s) 870, 1030
BmsI GCATC 4 cut(s) 812, 844, 890, 1034
BpiI GAAGAC 2 cut(s) 491, 825
BpuMI CCSGG 1 cut(s) 1030
BsaHI GRCGYC 1 cut(s) 288
BsaJI CCNNGG 2 cut(s) 597, 869
BsaWI WCCGGW 2 cut(s) 383, 494
Bsc4I CCNNNNNNNGG 3 cut(s) 434, 501, 785
Bse1I ACTGG 2 cut(s) 45, 67
Bse21I CCTNAGG 1 cut(s) 252
BseBI CCWGG 1 cut(s) 870
BseDI CCNNGG 2 cut(s) 597, 869
BseGI GGATG 5 cut(s) 41, 56, 825, 859, 1025
BseLI CCNNNNNNNGG 3 cut(s) 434, 501, 785
BseMII CTCAG 2 cut(s) 243, 615
BseNI ACTGG 2 cut(s) 45, 67
BseXI GCAGC 2 cut(s) 514, 622
BsgI GTGCAG 1 cut(s) 513
BshFI GGCC 4 cut(s) 76, 161, 373, 1044
BshNI GGYRCC 1 cut(s) 380
BsiHKCI CYCGRG 1 cut(s) 596
BsiSI CCGG 3 cut(s) 384, 495, 1029
BslI CCNNNNNNNGG 3 cut(s) 434, 501, 785
BsmAI GTCTC 1 cut(s) 279
BsmBI CGTCTC 1 cut(s) 279
BsnI GGCC 4 cut(s) 76, 161, 373, 1044
BsoBI CYCGRG 1 cut(s) 596
Bsp1286I GDGCHC 1 cut(s) 135
Bsp1407I TGTACA 1 cut(s) 444
Bsp143I GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
BspACI CCGC 2 cut(s) 178, 829
BspANI GGCC 4 cut(s) 76, 161, 373, 1044
BspCNI CTCAG 2 cut(s) 244, 616
BspHI TCATGA 1 cut(s) 982
BspLI GGNNCC 3 cut(s) 162, 249, 382
BspMAI CTGCAG 1 cut(s) 640
BspMI ACCTGC 1 cut(s) 183
BspPI GGATC 3 cut(s) 609, 711, 788
BspQI GCTCTTC 1 cut(s) 123
BspT107I GGYRCC 1 cut(s) 380
BsrBI CCGCTC 1 cut(s) 831
BsrGI TGTACA 1 cut(s) 444
BsrI ACTGG 2 cut(s) 45, 67
BssECI CCNNGG 2 cut(s) 597, 869
BssMI GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
BssNI GRCGYC 1 cut(s) 288
Bst2UI CCWGG 1 cut(s) 870
Bst4CI ACNGT 4 cut(s) 397, 550, 811, 1020
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 1 cut(s) 288
BstAUI TGTACA 1 cut(s) 444
BstC8I GCNNGC 1 cut(s) 593
BstDEI CTNAG 2 cut(s) 252, 624
BstEII GGTNACC 1 cut(s) 666
BstF5I GGATG 5 cut(s) 41, 56, 825, 859, 1025
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 1 cut(s) 527
BstKTI GATC 8 cut(s) 99, 276, 477, 604, 664, 719, 796, 879
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
BstMWI GCNNNNNNNGC 2 cut(s) 158, 760
BstNI CCWGG 1 cut(s) 870
BstNSI RCATGY 1 cut(s) 595
BstPI GGTNACC 1 cut(s) 666
BstSCI CCNGG 2 cut(s) 868, 1028
BstSFI CTRYAG 2 cut(s) 393, 636
BstV1I GCAGC 2 cut(s) 514, 622
BstV2I GAAGAC 2 cut(s) 491, 825
Bsu36I CCTNAGG 1 cut(s) 252
BsuRI GGCC 4 cut(s) 76, 161, 373, 1044
BtgZI GCGATG 2 cut(s) 198, 495
BtsCI GGATG 5 cut(s) 41, 56, 825, 859, 1025
BtsIMutI CAGTG 3 cut(s) 27, 52, 60
BveI ACCTGC 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 593
CciI TCATGA 1 cut(s) 982
CfoI GCGC 1 cut(s) 527
Cfr13I GGNCC 4 cut(s) 75, 160, 371, 1043
Csp6I GTAC 2 cut(s) 445, 912
CviAII CATG 3 cut(s) 347, 592, 983
CviQI GTAC 2 cut(s) 445, 912
DdeI CTNAG 2 cut(s) 252, 624
DpnI GATC 8 cut(s) 98, 275, 476, 603, 663, 718, 795, 878
DpnII GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
DraI TTTAAA 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 135
Eco32I GATATC 1 cut(s) 492
Eco47III AGCGCT 1 cut(s) 526
Eco57I CTGAAG 1 cut(s) 422
Eco81I CCTNAGG 1 cut(s) 252
Eco88I CYCGRG 1 cut(s) 596
Eco91I GGTNACC 1 cut(s) 666
EcoO65I GGTNACC 1 cut(s) 666
EcoRI GAATTC 2 cut(s) 16, 986
EcoRII CCWGG 1 cut(s) 868
EcoRV GATATC 1 cut(s) 492
EcoT38I GRGCYC 1 cut(s) 135
Esp3I CGTCTC 1 cut(s) 279
FaeI CATG 3 cut(s) 350, 595, 986
FatI CATG 3 cut(s) 346, 591, 982
FbaI TGATCA 1 cut(s) 474
FblI GTMKAC 1 cut(s) 291
Fnu4HI GCNGC 2 cut(s) 528, 636
FokI GGATG 5 cut(s) 28, 43, 812, 866, 1012
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 2 cut(s) 528, 636
GlaI GCGC 1 cut(s) 526
GluI GCNGC 2 cut(s) 528, 636
HaeII RGCGCY 1 cut(s) 528
HaeIII GGCC 4 cut(s) 76, 161, 373, 1044
HapII CCGG 3 cut(s) 384, 495, 1029
HhaI GCGC 1 cut(s) 527
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 3 cut(s) 350, 595, 986
Hin6I GCGC 1 cut(s) 525
HinP1I GCGC 1 cut(s) 525
HincII GTYRAC 1 cut(s) 931
HindII GTYRAC 1 cut(s) 931
HindIII AAGCTT 1 cut(s) 614
HinfI GANTC 5 cut(s) 188, 212, 260, 539, 979
HpaII CCGG 3 cut(s) 384, 495, 1029
HphI GGTGA 4 cut(s) 269, 362, 893, 982
Hpy166II GTNNAC 3 cut(s) 292, 445, 931
Hpy188I TCNGA 8 cut(s) 226, 265, 273, 402, 504, 522, 676, 920
Hpy188III TCNNGA 7 cut(s) 245, 337, 428, 554, 851, 862, 983
Hpy8I GTNNAC 3 cut(s) 292, 445, 931
HpyAV CCTTC 3 cut(s) 174, 384, 681
HpyCH4III ACNGT 4 cut(s) 397, 550, 811, 1020
HpyCH4IV ACGT 2 cut(s) 141, 288
HpyCH4V TGCA 7 cut(s) 89, 530, 611, 638, 803, 857, 1025
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 760
HpyF3I CTNAG 2 cut(s) 252, 624
HpySE526I ACGT 2 cut(s) 141, 288
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 3 cut(s) 350, 595, 986
HspAI GCGC 1 cut(s) 525
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
LguI GCTCTTC 1 cut(s) 123
LmnI GCTCC 4 cut(s) 180, 247, 430, 676
Lsp1109I GCAGC 2 cut(s) 514, 622
LweI GCATC 4 cut(s) 812, 844, 890, 1034
MaeII ACGT 2 cut(s) 141, 288
MaeIII GTNAC 4 cut(s) 113, 619, 666, 895
MalI GATC 8 cut(s) 98, 275, 476, 603, 663, 718, 795, 878
MbiI CCGCTC 1 cut(s) 831
MboI GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
MboII GAAGA 7 cut(s) 140, 415, 491, 563, 671, 706, 830
MfeI CAATTG 1 cut(s) 100
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 8 cut(s) 16, 100, 203, 366, 583, 606, 723, 986
MlyI GAGTC 1 cut(s) 973
MmeI TCCRAC 1 cut(s) 340
MseI TTAA 4 cut(s) 219, 233, 647, 1050
MspA1I CMGCKG 1 cut(s) 785
MspI CCGG 3 cut(s) 384, 495, 1029
MspR9I CCNGG 2 cut(s) 870, 1030
MunI CAATTG 1 cut(s) 100
MvaI CCWGG 1 cut(s) 870
MwoI GCNNNNNNNGC 2 cut(s) 158, 760
NciI CCSGG 1 cut(s) 1030
NdeII GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
NlaIII CATG 3 cut(s) 350, 595, 986
NlaIV GGNNCC 3 cut(s) 162, 249, 382
NmeAIII GCCGAG 1 cut(s) 52
NmuCI GTSAC 2 cut(s) 113, 619
NspI RCATGY 1 cut(s) 595
PaeI GCATGC 1 cut(s) 595
PagI TCATGA 1 cut(s) 982
PciSI GCTCTTC 1 cut(s) 123
PcsI WCGNNNNNNNCGW 1 cut(s) 79
PfeI GAWTC 4 cut(s) 188, 212, 260, 539
PflMI CCANNNNNTGG 1 cut(s) 785
PfoI TCCNGGA 1 cut(s) 1028
PkrI GCNGC 2 cut(s) 529, 637
PleI GAGTC 1 cut(s) 973
PpsI GAGTC 1 cut(s) 973
Psp1406I AACGTT 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 868
PspEI GGTNACC 1 cut(s) 666
PspGI CCWGG 1 cut(s) 868
PspN4I GGNNCC 3 cut(s) 162, 249, 382
PspPI GGNCC 4 cut(s) 75, 160, 371, 1043
PstI CTGCAG 1 cut(s) 640
PvuII CAGCTG 1 cut(s) 785
RsaI GTAC 2 cut(s) 446, 913
RsaNI GTAC 2 cut(s) 445, 912
SapI GCTCTTC 1 cut(s) 123
SaqAI TTAA 4 cut(s) 219, 233, 647, 1050
SatI GCNGC 2 cut(s) 528, 636
Sau3AI GATC 8 cut(s) 96, 273, 474, 601, 661, 716, 793, 876
Sau96I GGNCC 4 cut(s) 75, 160, 371, 1043
SchI GAGTC 1 cut(s) 973
ScrFI CCNGG 2 cut(s) 870, 1030
SduI GDGCHC 1 cut(s) 135
SfaNI GCATC 4 cut(s) 812, 844, 890, 1034
SfcI CTRYAG 2 cut(s) 393, 636
SmiI ATTTAAAT 1 cut(s) 220
SphI GCATGC 1 cut(s) 595
Sse9I AATT 8 cut(s) 16, 100, 203, 366, 583, 606, 723, 986
SsiI CCGC 2 cut(s) 178, 829
SspI AATATT 1 cut(s) 1055
StyD4I CCNGG 2 cut(s) 868, 1028
SwaI ATTTAAAT 1 cut(s) 220
TaaI ACNGT 4 cut(s) 397, 550, 811, 1020
TaiI ACGT 2 cut(s) 144, 291
TaqI TCGA 8 cut(s) 14, 82, 276, 451, 457, 587, 715, 861
TasI AATT 8 cut(s) 16, 100, 203, 366, 583, 606, 723, 986
TatI WGTACW 1 cut(s) 444
TfiI GAWTC 4 cut(s) 188, 212, 260, 539
Tru1I TTAA 4 cut(s) 219, 233, 647, 1050
Tru9I TTAA 4 cut(s) 219, 233, 647, 1050
TscAI CASTG 3 cut(s) 27, 52, 67
TseFI GTSAC 2 cut(s) 113, 619
TseI GCWGC 2 cut(s) 527, 635
Tsp45I GTSAC 2 cut(s) 113, 619
TspDTI ATGAA 6 cut(s) 201, 204, 594, 707, 827, 999
TspGWI ACGGA 1 cut(s) 181
TspRI CASTG 3 cut(s) 27, 52, 67
Van91I CCANNNNNTGG 1 cut(s) 785
XapI RAATTY 4 cut(s) 16, 203, 583, 986
XceI RCATGY 1 cut(s) 595
XmiI GTMKAC 1 cut(s) 291
ZraI GACGTC 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.