pycom133g00170

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000133
OG0012994
Physical Location & Seq
Forward (+)
368105 .. 369803
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
ATGTCTCCAATTGTTCTTCTCCTTCTTCTGTCTCTTTCCATGCATGCATGCAACGGCCAACTTCTAGGCTTCCTTCCTAATGAAAGTGGCCATAGAGCCTACCATTCTGTTGAGGATGTTGGGAATGTCTTAAGACGTTCTGAGACTTCAATGTCGTCGACGAGGCCTTCCATCTCAGTAGAGTTCCAATCAAAAGAAGTAGAAGTGAAAGACGCTGAAACAGTACTCACCCTTGAGAGTATTCCAAACGATGATGGTTCGAGGAAACGAGGACATGTTGATTTTGGACCAACTTCAGGACAGGATATTTTTACATTTTCTCAGATTCACCAGAAGAAATCGATTAAAGTTAAGGGATTGGAGAGGCAGACAAGGACATTACTGGGATCTGCTACGCTGCATGATCAGATGGAGGAAGATAAAGATCCGAAAGGAAACGAAGCTACGGAGGACGTAGGTGTTATGGATTATGCACAACCTCACAGGAAACCACCCATTCACAATGAAAAGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

172

Amino Acids

18.98

Weight (kDa)

5.89

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16515 AT4G16515
fragaria_vesca FvH4_5g00070
malus_domestica MD00G1115000.v1.1
prunus_persica Prupe.5G072500_v2.0.a1 Prupe.5G072500_v2.0.a1
pyrus_communis pycom133g00170
rosa_chinensis RchiOBHm_Chr7g0202891
rosa_laevigata RLG00000003566
rosa_multiflora Rmu_sc0033032.1_g000001
rosa_roxburghii Rroxscaffold_3G00254200
rosa_rugosa Rorug07G0073600 Rorug07G0073700
rosa_samantha Rh7AG201400 Rh7BG201800 Rh7CG212900 Rh7DG208400
rosa_wichuraiana Rw7G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 151
AccI GTMKAC 1 cut(s) 158
AclWI GGATC 2 cut(s) 394, 419
AcoI YGGCCR 2 cut(s) 55, 88
AcuI CTGAAG 1 cut(s) 279
AfaI GTAC 1 cut(s) 225
AfiI CCNNNNNNNGG 1 cut(s) 296
AflII CTTAAG 1 cut(s) 130
AflIII ACRYGT 1 cut(s) 274
AgsI TTSAA 1 cut(s) 150
AluBI AGCT 1 cut(s) 443
AluI AGCT 1 cut(s) 443
Alw26I GTCTC 3 cut(s) 9, 36, 137
AlwI GGATC 2 cut(s) 394, 419
AoxI GGCC 3 cut(s) 55, 88, 164
ApeKI GCWGC 1 cut(s) 397
AspS9I GGNCC 1 cut(s) 287
AsuHPI GGTGA 2 cut(s) 220, 320
AvaII GGWCC 1 cut(s) 287
BalI TGGCCA 1 cut(s) 90
BbvI GCAGC 1 cut(s) 384
BccI CCATC 3 cut(s) 179, 248, 403
BceAI ACGGC 1 cut(s) 70
BclI TGATCA 1 cut(s) 403
BcoDI GTCTC 3 cut(s) 9, 36, 137
BfaI CTAG 1 cut(s) 65
BfrI CTTAAG 1 cut(s) 130
BisI GCNGC 1 cut(s) 398
BlsI GCNGC 1 cut(s) 399
BmcAI AGTACT 1 cut(s) 225
Bme18I GGWCC 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 287
BmrI ACTGGG 1 cut(s) 392
BmuI ACTGGG 1 cut(s) 392
BpuEI CTTGAG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 341
BsaBI GATNNNNATC 1 cut(s) 423
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse1I ACTGG 1 cut(s) 387
Bse8I GATNNNNATC 1 cut(s) 423
BseCI ATCGAT 1 cut(s) 341
BseGI GGATG 1 cut(s) 121
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMII CTCAG 3 cut(s) 132, 189, 335
BseNI ACTGG 1 cut(s) 387
BseXI GCAGC 1 cut(s) 384
BshFI GGCC 3 cut(s) 57, 90, 166
BshVI ATCGAT 1 cut(s) 341
BslI CCNNNNNNNGG 1 cut(s) 296
BsmAI GTCTC 3 cut(s) 9, 36, 137
BsnI GGCC 3 cut(s) 57, 90, 166
Bsp143I GATC 3 cut(s) 386, 403, 424
BspANI GGCC 3 cut(s) 57, 90, 166
BspCNI CTCAG 3 cut(s) 133, 188, 334
BspDI ATCGAT 1 cut(s) 341
BspPI GGATC 2 cut(s) 394, 419
BspTI CTTAAG 1 cut(s) 130
BsrI ACTGG 1 cut(s) 387
BssMI GATC 3 cut(s) 386, 403, 424
Bst4CI ACNGT 1 cut(s) 223
BstAFI CTTAAG 1 cut(s) 130
BstC8I GCNNGC 2 cut(s) 45, 49
BstDEI CTNAG 3 cut(s) 141, 175, 321
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 3 cut(s) 389, 406, 427
BstMAI GTCTC 3 cut(s) 9, 36, 137
BstMBI GATC 3 cut(s) 386, 403, 424
BstNSI RCATGY 3 cut(s) 47, 51, 278
BstV1I GCAGC 1 cut(s) 384
BstX2I RGATCY 2 cut(s) 386, 424
BstYI RGATCY 2 cut(s) 386, 424
Bsu15I ATCGAT 1 cut(s) 341
BsuRI GGCC 3 cut(s) 57, 90, 166
BsuTUI ATCGAT 1 cut(s) 341
BtsCI GGATG 1 cut(s) 121
Cac8I GCNNGC 2 cut(s) 45, 49
Cfr13I GGNCC 1 cut(s) 287
ClaI ATCGAT 1 cut(s) 341
CseI GACGC 1 cut(s) 221
Csp6I GTAC 1 cut(s) 224
CviAII CATG 5 cut(s) 40, 44, 48, 275, 401
CviJI RGCY 6 cut(s) 57, 69, 90, 98, 166, 443
CviKI_1 RGCY 6 cut(s) 57, 69, 90, 98, 166, 443
CviQI GTAC 1 cut(s) 224
DdeI CTNAG 3 cut(s) 141, 175, 321
DpnI GATC 3 cut(s) 388, 405, 426
DpnII GATC 3 cut(s) 386, 403, 424
DrdI GACNNNNNNGTC 1 cut(s) 151
DseDI GACNNNNNNGTC 1 cut(s) 151
EaeI YGGCCR 2 cut(s) 55, 88
Eco147I AGGCCT 1 cut(s) 166
Eco47I GGWCC 1 cut(s) 287
Eco57I CTGAAG 1 cut(s) 279
EcoT22I ATGCAT 2 cut(s) 45, 49
FaeI CATG 5 cut(s) 43, 47, 51, 278, 404
FaiI YATR 8 cut(s) 41, 45, 49, 93, 276, 402, 464, 471
FatI CATG 5 cut(s) 39, 43, 47, 274, 400
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 1 cut(s) 158
Fnu4HI GCNGC 1 cut(s) 398
FokI GGATG 1 cut(s) 128
Fsp4HI GCNGC 1 cut(s) 398
FspBI CTAG 1 cut(s) 65
GluI GCNGC 1 cut(s) 398
HaeIII GGCC 3 cut(s) 57, 90, 166
HgaI GACGC 1 cut(s) 221
Hin1II CATG 5 cut(s) 43, 47, 51, 278, 404
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HinfI GANTC 1 cut(s) 325
HphI GGTGA 2 cut(s) 220, 320
Hpy166II GTNNAC 1 cut(s) 159
Hpy188I TCNGA 4 cut(s) 142, 324, 408, 429
Hpy188III TCNNGA 1 cut(s) 297
Hpy8I GTNNAC 1 cut(s) 159
Hpy99I CGWCG 2 cut(s) 160, 163
HpyAV CCTTC 3 cut(s) 32, 83, 177
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 2 cut(s) 136, 453
HpyCH4V TGCA 5 cut(s) 43, 47, 51, 400, 473
HpyF3I CTNAG 3 cut(s) 141, 175, 321
HpySE526I ACGT 2 cut(s) 136, 453
Hsp92II CATG 5 cut(s) 43, 47, 51, 278, 404
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 3 cut(s) 386, 403, 424
LpnPI CCDG 5 cut(s) 282, 287, 344, 368, 469
Lsp1109I GCAGC 1 cut(s) 384
MaeI CTAG 1 cut(s) 65
MaeII ACGT 2 cut(s) 136, 453
MalI GATC 3 cut(s) 388, 405, 426
MboI GATC 3 cut(s) 386, 403, 424
MboII GAAGA 4 cut(s) 8, 17, 346, 428
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 2 cut(s) 386, 424
MlsI TGGCCA 1 cut(s) 90
MluCI AATT 1 cut(s) 9
MluNI TGGCCA 1 cut(s) 90
MnlI CCTC 8 cut(s) 106, 156, 255, 263, 357, 406, 442, 489
Mox20I TGGCCA 1 cut(s) 90
Mph1103I ATGCAT 2 cut(s) 45, 49
MscI TGGCCA 1 cut(s) 90
MseI TTAA 3 cut(s) 131, 345, 351
Msp20I TGGCCA 1 cut(s) 90
MspCI CTTAAG 1 cut(s) 130
MunI CAATTG 1 cut(s) 9
NdeII GATC 3 cut(s) 386, 403, 424
NlaIII CATG 5 cut(s) 43, 47, 51, 278, 404
NsiI ATGCAT 2 cut(s) 45, 49
NspI RCATGY 3 cut(s) 47, 51, 278
PaeI GCATGC 2 cut(s) 47, 51
PceI AGGCCT 1 cut(s) 166
PciI ACATGT 1 cut(s) 274
PfeI GAWTC 1 cut(s) 325
PkrI GCNGC 1 cut(s) 399
PscI ACATGT 1 cut(s) 274
PspPI GGNCC 1 cut(s) 287
PsuI RGATCY 2 cut(s) 386, 424
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
SalI GTCGAC 1 cut(s) 157
SaqAI TTAA 3 cut(s) 131, 345, 351
SatI GCNGC 1 cut(s) 398
Sau3AI GATC 3 cut(s) 386, 403, 424
Sau96I GGNCC 1 cut(s) 287
ScaI AGTACT 1 cut(s) 225
SetI ASST 5 cut(s) 139, 445, 456, 460, 481
SgrDI CGTCGACG 1 cut(s) 157
SinI GGWCC 1 cut(s) 287
SmlI CTYRAG 2 cut(s) 130, 233
SmoI CTYRAG 2 cut(s) 130, 233
SphI GCATGC 2 cut(s) 47, 51
Sse9I AATT 1 cut(s) 9
SseBI AGGCCT 1 cut(s) 166
SspMI CTAG 1 cut(s) 65
StuI AGGCCT 1 cut(s) 166
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 2 cut(s) 139, 456
TaqI TCGA 3 cut(s) 158, 260, 341
TasI AATT 1 cut(s) 9
TatI WGTACW 1 cut(s) 223
TfiI GAWTC 1 cut(s) 325
Tru1I TTAA 3 cut(s) 131, 345, 351
Tru9I TTAA 3 cut(s) 131, 345, 351
TseI GCWGC 1 cut(s) 397
TspDTI ATGAA 1 cut(s) 96
TspGWI ACGGA 1 cut(s) 461
Vha464I CTTAAG 1 cut(s) 130
VpaK11BI GGWCC 1 cut(s) 287
XceI RCATGY 3 cut(s) 47, 51, 278
XmiI GTMKAC 1 cut(s) 158
XspI CTAG 1 cut(s) 65
ZrmI AGTACT 1 cut(s) 225
Zsp2I ATGCAT 2 cut(s) 45, 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.