pycom13g00680

UPF0496 protein 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014732
Physical Location & Seq
Forward (+)
431228 .. 432271
1044 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1044 bp
ATGTACCTAACGGAAATCTCCAACTTCCCGTCACTCCTTCCCTTCCGCGGTGCTAAGAAACATTCCGTTCCATCCAATTTGGATCTCATCTCTAGCTCCTTCGATGACGCTGTGCTCCGCCGCTTGAAATCTCTCGGTCCGCCGTCCATCAGCTTGTCCTGGCTCTCCAAGGCCGTTGATTTTCTCGCCTCGACTCACTCCGAAGCGAGAAACCTCATCTCCGATCTCAAGGTCGCGCCGCCTCTCGATGACTCGCTCGCTTGGTATTTGGACAACAGCGTCAAGCTCTTGGACCTCTGCAACTCCATCTCTTCCGAGATCGAACGCCTTCGCCAGCGCCGCCTTCATCTCACCTTCGTCCTTCACCTTCTCGGCGACGGAAAGGGTGAAGAGCTCCCGTCGCCGGAGAATCTGCACCGATCTCGCAAATCTCTATCCGATTTTGATCGCGGTGCTTCTTCTGGCTTTGGAAAGCGGAGCAACGCCGAAGTCTTGGTCAGAGATCTGAACCTGGGCCTGACTCGCCTGAGCAACGCGCCACGTGGCAAGATCTCGAGCGTGGCAAAGCTTGTGCGTCGAACAGTGCGCGCGGTGGGACTGGTAACTGTGTTCATCGCTGGAATCGCGGTGTCGACAATTCACGGATCTCCGGATATGGTTAGGGTCCGAGCCTCGGCGGAGTTTCCATGGGCCGAGTCGTTCAACGAGTTTGAATCGGCAGTCTGGGCCGAGTTCAAACGGCGATCCGGGCGCAGCAATGATGAGAAACTGAGAGGGATGTTGGAAGAGTTGGATGACGTGGGAACACGTGTTAAGGATGCGTGTGGGGTCGTGGATGACCTGGCAGCAGTTGCGGAAGTGAACAAGGAAGAGAAGGAGCGGTTAGACGAGGTCGTAAAAGAGCTGGAGAAGGCAACGGCGTCGTTTTCTGATGGGTTGGATAGGTTGAGTAACGGAGTGAACGTGATGTTTGATGGTGTTTTGCGCAACAGAAACCATATGACGGAGAATGTTAGAGTTAGCGGAGTGGGCAAAAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

348

Amino Acids

38.31

Weight (kDa)

8.45

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 48 - 148 3.5e-09 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014732)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36680
malus_domestica MD13G1008300.v1.1
prunus_persica Prupe.1G344800_v2.0.a1
pyrus_communis pycom13g00680
rosa_chinensis RchiOBHm_Chr4g0446391
rosa_laevigata RLG00000005666
rosa_multiflora Rmu_sc0008360.1_g000003 Rmu_sc0010778.1_g000005 Rmu_sc0025512.1_g000002 Rmu_sc0025513.1_g000001
rosa_roxburghii Rroxscaffold_5G00386830
rosa_rugosa Rorug04G0371000
rosa_samantha Rh4AG432500 Rh4BG441700 Rh4CG459000 Rh4DG440400
rosa_wichuraiana Rw4G036940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
Acc16I TGCGCA 1 cut(s) 986
AccBSI CCGCTC 1 cut(s) 880
AccI GTMKAC 1 cut(s) 632
AccII CGCG 7 cut(s) 48, 236, 450, 536, 588, 590, 626
AccIII TCCGGA 1 cut(s) 649
AclWI GGATC 3 cut(s) 90, 652, 738
AcvI CACGTG 2 cut(s) 542, 809
AcyI GRCGYC 1 cut(s) 920
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 4 cut(s) 47, 403, 673, 1003
AflIII ACRYGT 2 cut(s) 806, 808
AgsI TTSAA 4 cut(s) 127, 703, 713, 736
AjiI CACGTC 1 cut(s) 799
AjnI CCWGG 3 cut(s) 158, 510, 840
AluBI AGCT 6 cut(s) 96, 153, 286, 394, 568, 904
AluI AGCT 6 cut(s) 96, 153, 286, 394, 568, 904
Alw21I GWGCWC 2 cut(s) 117, 396
AlwI GGATC 3 cut(s) 90, 652, 738
Ama87I CYCGRG 1 cut(s) 553
Aor13HI TCCGGA 1 cut(s) 649
AoxI GGCC 4 cut(s) 171, 514, 690, 726
ApeKI GCWGC 2 cut(s) 753, 845
ArsI GACNNNNNNTTYG 2 cut(s) 480, 512
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 7 cut(s) 238, 339, 538, 588, 590, 753, 987
AspS9I GGNCC 6 cut(s) 137, 292, 514, 664, 690, 726
AsuC2I CCSGG 1 cut(s) 748
AsuHPI GGTGA 3 cut(s) 343, 356, 398
AvaI CYCGRG 1 cut(s) 553
AvaII GGWCC 3 cut(s) 137, 292, 664
BanII GRGCYC 1 cut(s) 396
BbrPI CACGTG 2 cut(s) 542, 809
Bbv12I GWGCWC 2 cut(s) 117, 396
BbvI GCAGC 2 cut(s) 765, 857
BccI CCATC 5 cut(s) 79, 155, 314, 926, 968
BceAI ACGGC 4 cut(s) 127, 158, 755, 933
BcgI CGANNNNNNTGC 4 cut(s) 404, 438, 903, 937
BciT130I CCWGG 3 cut(s) 160, 512, 842
BcnI CCSGG 1 cut(s) 748
BfaI CTAG 1 cut(s) 93
BfoI RGCGCY 1 cut(s) 340
BglII AGATCT 2 cut(s) 502, 549
BisI GCNGC 5 cut(s) 121, 239, 340, 754, 846
BlsI GCNGC 5 cut(s) 122, 240, 341, 755, 847
Bme1390I CCNGG 4 cut(s) 160, 512, 748, 842
Bme18I GGWCC 3 cut(s) 137, 292, 664
BmeT110I CYCGRG 1 cut(s) 553
BmgBI CACGTC 1 cut(s) 799
BmgT120I GGNCC 6 cut(s) 137, 292, 514, 664, 690, 726
BmiI GGNNCC 1 cut(s) 665
BmrFI CCNGG 4 cut(s) 160, 512, 748, 842
BmsI GCATC 1 cut(s) 808
BpmI CTGGAG 1 cut(s) 926
Bpu10I CCTNAGC 1 cut(s) 527
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 748
BsaAI YACGTR 2 cut(s) 542, 809
BsaBI GATNNNNATC 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 920
BsaJI CCNNGG 5 cut(s) 46, 168, 511, 672, 686
BsaWI WCCGGW 1 cut(s) 649
BsaXI ACNNNNNCTCC 4 cut(s) 203, 233, 948, 978
Bsc4I CCNNNNNNNGG 4 cut(s) 47, 403, 673, 1003
Bse1I ACTGG 1 cut(s) 603
Bse3DI GCAATG 1 cut(s) 763
Bse8I GATNNNNATC 1 cut(s) 444
BseAI TCCGGA 1 cut(s) 649
BseBI CCWGG 3 cut(s) 160, 512, 842
BseDI CCNNGG 5 cut(s) 46, 168, 511, 672, 686
BseGI GGATG 5 cut(s) 71, 783, 799, 823, 841
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 4 cut(s) 47, 403, 673, 1003
BseMI GCAATG 1 cut(s) 763
BseMII CTCAG 2 cut(s) 518, 761
BseNI ACTGG 1 cut(s) 603
BsePI GCGCGC 1 cut(s) 586
BseXI GCAGC 2 cut(s) 765, 857
BsgI GTGCAG 1 cut(s) 398
Bsh1236I CGCG 7 cut(s) 48, 236, 450, 536, 588, 590, 626
BshFI GGCC 4 cut(s) 173, 516, 692, 728
BsiHKAI GWGCWC 2 cut(s) 117, 396
BsiHKCI CYCGRG 1 cut(s) 553
BsiSI CCGG 3 cut(s) 404, 650, 747
BslFI GGGAC 1 cut(s) 609
BslI CCNNNNNNNGG 4 cut(s) 47, 403, 673, 1003
BsmFI GGGAC 1 cut(s) 609
BsnI GGCC 4 cut(s) 173, 516, 692, 728
BsoBI CYCGRG 1 cut(s) 553
Bsp1286I GDGCHC 2 cut(s) 117, 396
Bsp13I TCCGGA 1 cut(s) 649
Bsp143I GATC 9 cut(s) 82, 223, 318, 419, 445, 502, 549, 644, 743
Bsp19I CCATGG 1 cut(s) 686
BspANI GGCC 4 cut(s) 173, 516, 692, 728
BspCNI CTCAG 2 cut(s) 519, 762
BspEI TCCGGA 1 cut(s) 649
BspFNI CGCG 7 cut(s) 48, 236, 450, 536, 588, 590, 626
BspLI GGNNCC 1 cut(s) 665
BspPI GGATC 3 cut(s) 90, 652, 738
BspQI GCTCTTC 1 cut(s) 384
BsrBI CCGCTC 1 cut(s) 880
BsrDI GCAATG 1 cut(s) 763
BsrI ACTGG 1 cut(s) 603
BssECI CCNNGG 5 cut(s) 46, 168, 511, 672, 686
BssHII GCGCGC 1 cut(s) 586
BssMI GATC 9 cut(s) 82, 223, 318, 419, 445, 502, 549, 644, 743
BssNI GRCGYC 1 cut(s) 920
BssT1I CCWWGG 2 cut(s) 168, 686
Bst2UI CCWGG 3 cut(s) 160, 512, 842
Bst4CI ACNGT 2 cut(s) 583, 607
Bst6I CTCTTC 4 cut(s) 316, 384, 780, 864
BstACI GRCGYC 1 cut(s) 920
BstAPI GCANNNNNTGC 1 cut(s) 851
BstBAI YACGTR 2 cut(s) 542, 809
BstC8I GCNNGC 3 cut(s) 258, 335, 588
BstDEI CTNAG 3 cut(s) 54, 527, 770
BstDSI CCRYGG 2 cut(s) 46, 686
BstF5I GGATG 5 cut(s) 71, 783, 799, 823, 841
BstFNI CGCG 7 cut(s) 48, 236, 450, 536, 588, 590, 626
BstH2I RGCGCY 1 cut(s) 340
BstHHI GCGC 7 cut(s) 238, 339, 538, 588, 590, 753, 987
BstKTI GATC 9 cut(s) 85, 226, 321, 422, 448, 505, 552, 647, 746
BstMBI GATC 9 cut(s) 82, 223, 318, 419, 445, 502, 549, 644, 743
BstMWI GCNNNNNNNGC 8 cut(s) 339, 400, 522, 623, 725, 748, 851, 1029
BstNI CCWGG 3 cut(s) 160, 512, 842
BstSCI CCNGG 4 cut(s) 158, 510, 746, 840
BstUI CGCG 7 cut(s) 48, 236, 450, 536, 588, 590, 626
BstV1I GCAGC 2 cut(s) 765, 857
BstX2I RGATCY 4 cut(s) 82, 502, 549, 644
BstYI RGATCY 4 cut(s) 82, 502, 549, 644
BsuRI GGCC 4 cut(s) 173, 516, 692, 728
BtgI CCRYGG 2 cut(s) 46, 686
BtgZI GCGATG 1 cut(s) 598
BtrI CACGTC 1 cut(s) 799
BtsCI GGATG 5 cut(s) 71, 783, 799, 823, 841
BtsIMutI CAGTG 1 cut(s) 588
Cac8I GCNNGC 3 cut(s) 258, 335, 588
CfoI GCGC 7 cut(s) 238, 339, 538, 588, 590, 753, 987
Cfr13I GGNCC 6 cut(s) 137, 292, 514, 664, 690, 726
Cfr42I CCGCGG 1 cut(s) 49
CpoI CGGWCCG 1 cut(s) 137
CseI GACGC 4 cut(s) 116, 268, 563, 909
Csp6I GTAC 1 cut(s) 4
CspI CGGWCCG 1 cut(s) 137
CviAII CATG 2 cut(s) 687, 1041
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 3 cut(s) 54, 527, 770
DpnI GATC 9 cut(s) 84, 225, 320, 421, 447, 504, 551, 646, 745
DpnII GATC 9 cut(s) 82, 223, 318, 419, 445, 502, 549, 644, 743
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
Eam1104I CTCTTC 4 cut(s) 316, 384, 780, 864
EarI CTCTTC 4 cut(s) 316, 384, 780, 864
EciI GGCGGA 3 cut(s) 107, 129, 692
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 2 cut(s) 168, 686
Eco24I GRGCYC 1 cut(s) 396
Eco47I GGWCC 3 cut(s) 137, 292, 664
Eco53kI GAGCTC 1 cut(s) 394
Eco72I CACGTG 2 cut(s) 542, 809
Eco88I CYCGRG 1 cut(s) 553
EcoICRI GAGCTC 1 cut(s) 394
EcoRII CCWGG 3 cut(s) 158, 510, 840
EcoT14I CCWWGG 2 cut(s) 168, 686
EcoT38I GRGCYC 1 cut(s) 396
ErhI CCWWGG 2 cut(s) 168, 686
FaeI CATG 2 cut(s) 690, 1044
FaiI YATR 5 cut(s) 656, 688, 999, 1001, 1042
FaqI GGGAC 1 cut(s) 609
FatI CATG 2 cut(s) 686, 1040
FauNDI CATATG 1 cut(s) 999
FblI GTMKAC 1 cut(s) 632
Fnu4HI GCNGC 5 cut(s) 121, 239, 340, 754, 846
FokI GGATG 5 cut(s) 58, 790, 806, 830, 848
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 5 cut(s) 121, 239, 340, 754, 846
FspBI CTAG 1 cut(s) 93
FspI TGCGCA 1 cut(s) 986
GlaI GCGC 7 cut(s) 237, 338, 537, 587, 589, 752, 986
GluI GCNGC 5 cut(s) 121, 239, 340, 754, 846
GsuI CTGGAG 1 cut(s) 926
HaeII RGCGCY 1 cut(s) 340
HaeIII GGCC 4 cut(s) 173, 516, 692, 728
HapII CCGG 3 cut(s) 404, 650, 747
HgaI GACGC 4 cut(s) 116, 268, 563, 909
HhaI GCGC 7 cut(s) 238, 339, 538, 588, 590, 753, 987
Hin1I GRCGYC 1 cut(s) 920
Hin1II CATG 2 cut(s) 690, 1044
Hin6I GCGC 7 cut(s) 236, 337, 536, 586, 588, 751, 985
HinP1I GCGC 7 cut(s) 236, 337, 536, 586, 588, 751, 985
HincII GTYRAC 1 cut(s) 633
HindII GTYRAC 1 cut(s) 633
HindIII AAGCTT 1 cut(s) 566
HinfI GANTC 7 cut(s) 193, 251, 409, 520, 621, 695, 713
HpaII CCGG 3 cut(s) 404, 650, 747
HphI GGTGA 3 cut(s) 343, 356, 398
Hpy166II GTNNAC 3 cut(s) 633, 862, 961
Hpy188I TCNGA 8 cut(s) 202, 223, 316, 439, 500, 507, 668, 931
Hpy188III TCNNGA 3 cut(s) 245, 553, 650
Hpy8I GTNNAC 3 cut(s) 633, 862, 961
Hpy99I CGWCG 4 cut(s) 380, 403, 579, 925
HpyCH4III ACNGT 2 cut(s) 583, 607
HpyCH4IV ACGT 4 cut(s) 541, 798, 808, 963
HpyCH4V TGCA 2 cut(s) 300, 415
HpyF10VI GCNNNNNNNGC 8 cut(s) 339, 400, 522, 623, 725, 748, 851, 1029
HpyF3I CTNAG 3 cut(s) 54, 527, 770
HpySE526I ACGT 4 cut(s) 541, 798, 808, 963
Hsp92I GRCGYC 1 cut(s) 920
Hsp92II CATG 2 cut(s) 690, 1044
HspAI GCGC 7 cut(s) 236, 337, 536, 586, 588, 751, 985
Kpn2I TCCGGA 1 cut(s) 649
KspI CCGCGG 1 cut(s) 49
Kzo9I GATC 9 cut(s) 82, 223, 318, 419, 445, 502, 549, 644, 743
LguI GCTCTTC 1 cut(s) 384
LmnI GCTCC 5 cut(s) 101, 120, 399, 477, 877
Lsp1109I GCAGC 2 cut(s) 765, 857
LweI GCATC 1 cut(s) 808
MaeI CTAG 1 cut(s) 93
MaeII ACGT 4 cut(s) 541, 798, 808, 963
MaeIII GTNAC 3 cut(s) 30, 601, 950
MalI GATC 9 cut(s) 84, 225, 320, 421, 447, 504, 551, 646, 745
MbiI CCGCTC 1 cut(s) 880
MboI GATC 9 cut(s) 82, 223, 318, 419, 445, 502, 549, 644, 743
MboII GAAGA 5 cut(s) 303, 401, 450, 797, 881
MflI RGATCY 4 cut(s) 82, 502, 549, 644
MhlI GDGCHC 2 cut(s) 117, 396
MluCI AATT 2 cut(s) 76, 636
MlyI GAGTC 4 cut(s) 187, 245, 514, 704
MmeI TCCRAC 4 cut(s) 45, 762, 771, 918
MnlI CCTC 7 cut(s) 199, 224, 252, 305, 682, 767, 883
MroI TCCGGA 1 cut(s) 649
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 1 cut(s) 813
MspA1I CMGCKG 1 cut(s) 48
MspI CCGG 3 cut(s) 404, 650, 747
MspR9I CCNGG 4 cut(s) 160, 512, 748, 842
MvaI CCWGG 3 cut(s) 160, 512, 842
MvnI CGCG 7 cut(s) 48, 236, 450, 536, 588, 590, 626
MwoI GCNNNNNNNGC 8 cut(s) 339, 400, 522, 623, 725, 748, 851, 1029
NciI CCSGG 1 cut(s) 748
NcoI CCATGG 1 cut(s) 686
NdeI CATATG 1 cut(s) 999
NdeII GATC 9 cut(s) 82, 223, 318, 419, 445, 502, 549, 644, 743
NlaIII CATG 2 cut(s) 690, 1044
NlaIV GGNNCC 1 cut(s) 665
NmeAIII GCCGAG 4 cut(s) 351, 653, 718, 754
NmuCI GTSAC 1 cut(s) 30
NsbI TGCGCA 1 cut(s) 986
PaeR7I CTCGAG 1 cut(s) 553
PauI GCGCGC 1 cut(s) 586
PciSI GCTCTTC 1 cut(s) 384
PcsI WCGNNNNNNNCGW 1 cut(s) 960
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 3 cut(s) 409, 621, 713
PflFI GACNNNGTC 1 cut(s) 890
PkrI GCNGC 5 cut(s) 122, 240, 341, 755, 847
PleI GAGTC 4 cut(s) 187, 245, 514, 703
PmaCI CACGTG 2 cut(s) 542, 809
PmlI CACGTG 2 cut(s) 542, 809
PpsI GAGTC 4 cut(s) 187, 245, 514, 703
Ppu21I YACGTR 2 cut(s) 542, 809
Psp124BI GAGCTC 1 cut(s) 396
Psp6I CCWGG 3 cut(s) 158, 510, 840
PspCI CACGTG 2 cut(s) 542, 809
PspGI CCWGG 3 cut(s) 158, 510, 840
PspN4I GGNNCC 1 cut(s) 665
PspPI GGNCC 6 cut(s) 137, 292, 514, 664, 690, 726
PsuI RGATCY 4 cut(s) 82, 502, 549, 644
PsyI GACNNNGTC 1 cut(s) 890
PteI GCGCGC 1 cut(s) 586
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
Rsr2I CGGWCCG 1 cut(s) 137
RsrII CGGWCCG 1 cut(s) 137
SacI GAGCTC 1 cut(s) 396
SacII CCGCGG 1 cut(s) 49
SalI GTCGAC 1 cut(s) 631
SapI GCTCTTC 1 cut(s) 384
SaqAI TTAA 1 cut(s) 813
SatI GCNGC 5 cut(s) 121, 239, 340, 754, 846
Sau3AI GATC 9 cut(s) 82, 223, 318, 419, 445, 502, 549, 644, 743
Sau96I GGNCC 6 cut(s) 137, 292, 514, 664, 690, 726
SchI GAGTC 4 cut(s) 187, 245, 514, 704
ScrFI CCNGG 4 cut(s) 160, 512, 748, 842
SduI GDGCHC 2 cut(s) 117, 396
SfaNI GCATC 1 cut(s) 808
Sfr274I CTCGAG 1 cut(s) 553
Sfr303I CCGCGG 1 cut(s) 49
SgrBI CCGCGG 1 cut(s) 49
SinI GGWCC 3 cut(s) 137, 292, 664
SlaI CTCGAG 1 cut(s) 553
SmlI CTYRAG 2 cut(s) 227, 553
SmoI CTYRAG 2 cut(s) 227, 553
Sse9I AATT 2 cut(s) 76, 636
SspMI CTAG 1 cut(s) 93
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 4 cut(s) 158, 510, 746, 840
StyI CCWWGG 2 cut(s) 168, 686
TaaI ACNGT 2 cut(s) 583, 607
TaiI ACGT 4 cut(s) 544, 801, 811, 966
TaqI TCGA 7 cut(s) 102, 191, 246, 321, 554, 577, 632
TaqII GACCGA 1 cut(s) 125
TasI AATT 2 cut(s) 76, 636
TauI GCSGC 3 cut(s) 123, 241, 342
TfiI GAWTC 3 cut(s) 409, 621, 713
Tru1I TTAA 1 cut(s) 813
Tru9I TTAA 1 cut(s) 813
TscAI CASTG 1 cut(s) 588
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 2 cut(s) 753, 845
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 2 cut(s) 335, 601
TspGWI ACGGA 6 cut(s) 26, 55, 393, 657, 969, 1019
TspRI CASTG 1 cut(s) 588
Tth111I GACNNNGTC 1 cut(s) 890
VpaK11BI GGWCC 3 cut(s) 137, 292, 664
XhoI CTCGAG 1 cut(s) 553
XmiI GTMKAC 1 cut(s) 632
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.