pycom13g00770
NAC Family

Inactive leucine-rich repeat receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012108
Physical Location & Seq
Reverse (-)
480400 .. 484518
4119 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2097 bp
ATGTCGTGTTCCCAAGCCCCACAGTTTTGGTGTCTCCCCATTGATCAGTGCGCAGAGCTCGCCAATGGGGCTTTCTGCACCGCTCCTCCTCCGCAGCTGGGAGTAGAATTTTTAAAAATGGTTTCTCAGAAAGGTCGAATCTTTTTCTTATTGCTGCCATTGCTGTTCTGGGGTTTTCTGGGTTCGGCAGAAGCAGGAGATGCGGAGGCTCTGTTAACTCTGAAATCTACAATCGACCCTCAAAGCGTGCTGCCATGGCGGCAGGGAAGCAATGTGTGCGAGTGGGAAGGCGTGAAGGACTGCCTGCGAGGCAGAGTCACGAAGCTTGTGCTGGAATATTCGAACCTGACGGGGGTTTTGGATCACAAGATCTTGAACCGGCTCGACCAGCTGCGAGTCCTCAGCTTCAAAGGCAACTCCCTCTCCGGCCAAATCCCCGACCTCTCCGGCCTCCTCAACCTCAAATCCCTCTTCCTAAACGACAACAACTTCTCCGGCGTTTTCCCCTCTTCCATTTCCGACCTCCACCGCCTCAAAATCGTCGTCCTCGCTGGAAACAAAATCTCCGGCGAAATCCCAACGTCACTGCTCAGGCTCCGCCGCCTCTACGTCCTGTCCTTGCAGGACAACAAGTTCACCGGTCCCATTCCACCGCTCAACCAGACCTCCCTCCGGTTCTTCAACGTGTCGAATAACCAGCTATCCGGAGAAATTCCGGTGACCCCAACTCTGATTCTGTTCAATGCGTCGTCGTTTTTGGGGAACGTCTATGTCTGCGGGGTGCAACTTGACAAACCGTGCGGGGGCATTATCGCGTTTCCGCCGTCGATGAGTCCCACCAGTCAACCGGGTCCAATGTCAAAGTCAAAGCGCGGGAAACTGATTAAGATAATCGCAGGGAGCGTGGGCGGGTTCACGGTGGTCCTAATCTGCTCGGTTCTGCTGTGGATGGTGTGCCGGAGGAGTCGCGGCGGTGGCGGGGAGGCGAGGAGGAGCAAAGGCGGGGTTGTTGGGGGTGCTGAGCTGGCAGCAGAGAGAGGAGGAGGATCAGGGGAGGGGCCCAGTGGCAAGGGAGGTGATAATGGTGGGTTTTCGTGGGAAGGTGAGGGGTTGGGGAGTTTGGTGTTCTGCGGAGCAGGGGATCAGCAGATGGGTTACCGTCTGGAGGATTTGCTCAAGGCGTCGGCGGAGACGCTGGGGAGGGGCACAATGGGAAGTACGTACAAGGCCGTGATGGAGTCCGGGTTTATCGTGACGGTGAAGCGGTTGAAGGACGCGATGTACCCGAGGATGGACGAGTTTAGGAGACACGTGGACTTGCTAGGGAAGCTGAGGCACCCGCACCTGGTCCCACTGAGGGCCTACTTCCAGGCTAAGGAGGAACGCCTGCTCGTATACGATTATTTCCCCAATGGCAGCCTCTTTTCACTTATTCATGGTTCAAGAACTTCAGGAGGCGGGAAGCCGCTTCACTGGACGTCATGTCTTAAAATAGCTGAGGACTTGGCGAGTGGTGTTGTTTATATCCACCAGAACCCCGGCTTAACTCATGGAAACTTGAAATCCTCAAACGTCTTGTTAGGTTCCGATTTTGAATCCTGCTTGACGGATTATGGTCTCACGTTATTCCGAGATCCGAATTCACTCGAAGAACCTAGCGCAAGCACTCTCTTTTATAGAGCACCAGAATGCCGCGACATCAGAAAACCATCCACCCAACCAGCCGATGTGTACAGCTTTGGCGTCCTCCTCCTGGAGCTCCTAACCGGAAAGACTCCCTTCCAAGACCTTGTCCAGGAACATGGTTCGGACATCCCTAGGTGGGTCCGCTCGGTGCGCGAAGAAGAGACAGAGTCAGGGGATGACCCTGCCTCGGGGAATGAGGCATCAGAGGAGAAGCTGCAGGCACTTCTTAACATAGCAATGGCTTGTGTTGCGCTATCACCGGAGAACCGACCAGCGATGAGAGATGTTCTAAGGATGATAAGGGATGCTAGAGCGGAGGGTCACGTGTCTTCCAACAGCAGTGATCACTCGCCGGGGAGATGGTCGGATACCGTTCAGAGCCTGCCAAGAGAAGAACACTTGAGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

699

Amino Acids

75.92

Weight (kDa)

7.53

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 125 - 210 2.1e-06 Leucine-rich repeat region
LRR_8 PF13855 153 - 212 5.2e-07 Leucine rich repeat
Pkinase PF00069 396 - 660 1.3e-33 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 397 - 660 7.9e-31 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10850
fragaria_vesca FvH4_4g36460
malus_domestica MD13G1009100.v1.1 MD16G1007100.v1.1
prunus_persica Prupe.1G343600_v2.0.a1
pyrus_communis pycom13g00770 pycom16g00670
rosa_chinensis RchiOBHm_Chr4g0446221
rosa_laevigata RLG00000005680
rosa_multiflora Rmu_co8398623.1_g000001 Rmu_sc0002266.1_g000012 Rmu_sc0040593.1_g000001
rosa_roxburghii Rroxscaffold_5G00386620
rosa_rugosa Rorug04G0368500
rosa_samantha Rh4AG430700 Rh4BG440100 Rh4CG457400 Rh4DG438600
rosa_wichuraiana Rw4G036790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1481
Acc16I TGCGCA 1 cut(s) 52
AccB1I GGYRCC 1 cut(s) 1335
AccBSI CCGCTC 4 cut(s) 83, 655, 1830, 2000
AccI GTMKAC 1 cut(s) 1395
AccII CGCG 6 cut(s) 815, 873, 969, 1277, 1695, 1839
AccIII TCCGGA 1 cut(s) 704
AclWI GGATC 4 cut(s) 369, 1054, 1149, 1628
AcoI YGGCCR 1 cut(s) 427
AcsI RAATTY 3 cut(s) 107, 711, 1639
AcuI CTGAAG 1 cut(s) 1434
AcvI CACGTG 2 cut(s) 1312, 2011
AcyI GRCGYC 3 cut(s) 1181, 1478, 1743
AfaI GTAC 4 cut(s) 1219, 1223, 1283, 1733
AflIII ACRYGT 3 cut(s) 684, 1309, 2010
AgeI ACCGGT 1 cut(s) 638
AgsI TTSAA 8 cut(s) 376, 409, 682, 742, 1270, 1443, 1561, 1595
AhdI GACNNNNNGTC 1 cut(s) 1482
AjnI CCWGG 4 cut(s) 1344, 1368, 1752, 1794
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AloI GAACNNNNNNTCC 2 cut(s) 617, 649
Alw21I GWGCWC 3 cut(s) 60, 1684, 1761
Alw26I GTCTC 5 cut(s) 38, 1184, 1300, 1622, 1841
AlwI GGATC 4 cut(s) 369, 1054, 1149, 1628
AlwNI CAGNNNCTG 1 cut(s) 2068
Ama87I CYCGRG 2 cut(s) 1285, 1873
Aor13HI TCCGGA 1 cut(s) 704
AoxI GGCC 5 cut(s) 427, 448, 1058, 1227, 1359
ApaI GGGCCC 1 cut(s) 1062
ApeKI GCWGC 7 cut(s) 94, 154, 250, 391, 1028, 1416, 1900
ApoI RAATTY 3 cut(s) 107, 711, 1639
ArsI GACNNNNNNTTYG 4 cut(s) 340, 372, 528, 560
AsiGI ACCGGT 1 cut(s) 638
AspA2I CCTAGG 1 cut(s) 1817
AspLEI GCGC 5 cut(s) 53, 873, 1661, 1839, 1939
AspS9I GGNCC 8 cut(s) 641, 851, 922, 1058, 1059, 1348, 1359, 1825
AsuC2I CCSGG 4 cut(s) 849, 1243, 1539, 2040
AsuHPI GGTGA 6 cut(s) 628, 730, 1088, 1115, 1270, 1935
AsuII TTCGAA 1 cut(s) 341
AvaI CYCGRG 2 cut(s) 1285, 1873
AvaII GGWCC 5 cut(s) 641, 851, 922, 1348, 1825
AvrII CCTAGG 1 cut(s) 1817
BaeGI GKGCMC 2 cut(s) 1062, 1208
BanI GGYRCC 1 cut(s) 1335
BanII GRGCYC 3 cut(s) 60, 1062, 1761
BbrPI CACGTG 2 cut(s) 1312, 2011
BbsI GAAGAC 1 cut(s) 2007
Bbv12I GWGCWC 3 cut(s) 60, 1684, 1761
BbvCI CCTCAGC 3 cut(s) 401, 1331, 1497
BbvI GCAGC 7 cut(s) 106, 141, 237, 378, 1040, 1428, 1887
BccI CCATC 6 cut(s) 943, 1144, 1228, 1285, 1717, 2040
BceAI ACGGC 2 cut(s) 808, 1214
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 4 cut(s) 1346, 1370, 1754, 1796
BciVI GTATCC 1 cut(s) 2047
BclI TGATCA 2 cut(s) 43, 2029
BcnI CCSGG 4 cut(s) 849, 1243, 1539, 2040
BcoDI GTCTC 5 cut(s) 38, 1184, 1300, 1622, 1841
BfaI CTAG 4 cut(s) 1322, 1656, 1818, 1995
BfmI CTRYAG 1 cut(s) 1901
BfuI GTATCC 1 cut(s) 2047
BglI GCCNNNNNGGC 3 cut(s) 68, 259, 309
BglII AGATCT 1 cut(s) 369
BlnI CCTAGG 1 cut(s) 1817
BlpI GCTNAGC 1 cut(s) 1020
Bme1390I CCNGG 8 cut(s) 849, 1243, 1346, 1370, 1539, 1754, 1796, 2040
Bme18I GGWCC 5 cut(s) 641, 851, 922, 1348, 1825
BmeRI GACNNNNNGTC 1 cut(s) 1482
BmeT110I CYCGRG 2 cut(s) 1285, 1873
BmgT120I GGNCC 8 cut(s) 641, 851, 922, 1058, 1059, 1348, 1359, 1825
BmiI GGNNCC 9 cut(s) 596, 643, 852, 1059, 1060, 1337, 1350, 1585, 1826
BmrFI CCNGG 8 cut(s) 849, 1243, 1346, 1370, 1539, 1754, 1796, 2040
BmrI ACTGGG 1 cut(s) 1056
BmsI GCATC 3 cut(s) 190, 1895, 1981
BmuI ACTGGG 1 cut(s) 1056
BpiI GAAGAC 1 cut(s) 2007
BpmI CTGGAG 2 cut(s) 1184, 1775
Bpu10I CCTNAGC 5 cut(s) 401, 590, 1331, 1374, 1497
Bpu1102I GCTNAGC 1 cut(s) 1020
Bpu14I TTCGAA 1 cut(s) 341
BpuEI CTTGAG 1 cut(s) 1160
BpuMI CCSGG 4 cut(s) 849, 1243, 1539, 2040
BsaAI YACGTR 3 cut(s) 1221, 1312, 2011
BsaHI GRCGYC 3 cut(s) 1181, 1478, 1743
BsaI GGTCTC 1 cut(s) 1622
BsaJI CCNNGG 6 cut(s) 254, 1286, 1537, 1817, 1872, 2039
BsaWI WCCGGW 6 cut(s) 638, 672, 704, 715, 1766, 1945
Bse118I RCCGGY 2 cut(s) 378, 638
Bse1I ACTGG 3 cut(s) 840, 1062, 1478
Bse3DI GCAATG 3 cut(s) 158, 277, 1929
BseAI TCCGGA 1 cut(s) 704
BseBI CCWGG 4 cut(s) 1346, 1370, 1754, 1796
BseDI CCNNGG 6 cut(s) 254, 1286, 1537, 1817, 1872, 2039
BseGI GGATG 7 cut(s) 954, 1296, 1709, 1812, 1867, 1986, 1996
BseMI GCAATG 3 cut(s) 158, 277, 1929
BseMII CTCAG 7 cut(s) 140, 415, 604, 1011, 1322, 1346, 1488
BseNI ACTGG 3 cut(s) 840, 1062, 1478
BseSI GKGCMC 2 cut(s) 1062, 1208
BseXI GCAGC 7 cut(s) 106, 141, 237, 378, 1040, 1428, 1887
BseYI CCCAGC 2 cut(s) 97, 1195
BsgI GTGCAG 1 cut(s) 61
Bsh1236I CGCG 6 cut(s) 815, 873, 969, 1277, 1695, 1839
BshFI GGCC 5 cut(s) 429, 450, 1060, 1229, 1361
BshNI GGYRCC 1 cut(s) 1335
BshTI ACCGGT 1 cut(s) 638
BsiHKAI GWGCWC 3 cut(s) 60, 1684, 1761
BsiHKCI CYCGRG 2 cut(s) 1285, 1873
BslFI GGGAC 3 cut(s) 627, 819, 1334
BsmAI GTCTC 5 cut(s) 38, 1184, 1300, 1622, 1841
BsmBI CGTCTC 1 cut(s) 1184
BsmFI GGGAC 3 cut(s) 627, 819, 1334
BsmI GAATGC 1 cut(s) 1694
BsnI GGCC 5 cut(s) 429, 450, 1060, 1229, 1361
Bso31I GGTCTC 1 cut(s) 1622
BsoBI CYCGRG 2 cut(s) 1285, 1873
Bsp119I TTCGAA 1 cut(s) 341
Bsp120I GGGCCC 1 cut(s) 1058
Bsp1286I GDGCHC 5 cut(s) 60, 1062, 1208, 1684, 1761
Bsp13I TCCGGA 1 cut(s) 704
Bsp1407I TGTACA 1 cut(s) 1731
Bsp143I GATC 7 cut(s) 43, 361, 369, 1046, 1141, 1633, 2029
Bsp1720I GCTNAGC 1 cut(s) 1020
Bsp19I CCATGG 1 cut(s) 254
BspANI GGCC 5 cut(s) 429, 450, 1060, 1229, 1361
BspCNI CTCAG 7 cut(s) 139, 414, 603, 1012, 1323, 1347, 1489
BspEI TCCGGA 1 cut(s) 704
BspFNI CGCG 6 cut(s) 815, 873, 969, 1277, 1695, 1839
BspLI GGNNCC 9 cut(s) 596, 643, 852, 1059, 1060, 1337, 1350, 1585, 1826
BspMAI CTGCAG 1 cut(s) 1905
BspPI GGATC 4 cut(s) 369, 1054, 1149, 1628
BspT104I TTCGAA 1 cut(s) 341
BspT107I GGYRCC 1 cut(s) 1335
BspTNI GGTCTC 1 cut(s) 1622
BsrBI CCGCTC 4 cut(s) 83, 655, 1830, 2000
BsrDI GCAATG 3 cut(s) 158, 277, 1929
BsrFI RCCGGY 2 cut(s) 378, 638
BsrGI TGTACA 1 cut(s) 1731
BsrI ACTGG 3 cut(s) 840, 1062, 1478
BssAI RCCGGY 2 cut(s) 378, 638
BssECI CCNNGG 6 cut(s) 254, 1286, 1537, 1817, 1872, 2039
BssMI GATC 7 cut(s) 43, 361, 369, 1046, 1141, 1633, 2029
BssNAI GTATAC 1 cut(s) 1396
BssNI GRCGYC 3 cut(s) 1181, 1478, 1743
BssT1I CCWWGG 2 cut(s) 254, 1817
Bst1107I GTATAC 1 cut(s) 1396
Bst2UI CCWGG 4 cut(s) 1346, 1370, 1754, 1796
Bst4CI ACNGT 6 cut(s) 24, 798, 919, 1160, 1258, 2059
Bst6I CTCTTC 3 cut(s) 476, 514, 1839
BstACI GRCGYC 3 cut(s) 1181, 1478, 1743
BstAPI GCANNNNNTGC 2 cut(s) 200, 276
BstAUI TGTACA 1 cut(s) 1731
BstBAI YACGTR 3 cut(s) 1221, 1312, 2011
BstBI TTCGAA 1 cut(s) 341
BstC8I GCNNGC 8 cut(s) 60, 248, 305, 1026, 1388, 1663, 1905, 2069
BstDEI CTNAG 9 cut(s) 126, 401, 590, 1020, 1331, 1355, 1374, 1497, 1976
BstDSI CCRYGG 1 cut(s) 254
BstEII GGTNACC 2 cut(s) 718, 1154
BstENI CCTNNNNNAGG 1 cut(s) 1793
BstF5I GGATG 7 cut(s) 954, 1296, 1709, 1812, 1867, 1986, 1996
BstFNI CGCG 6 cut(s) 815, 873, 969, 1277, 1695, 1839
BstHHI GCGC 5 cut(s) 53, 873, 1661, 1839, 1939
BstKTI GATC 7 cut(s) 46, 364, 372, 1049, 1144, 1636, 2032
BstMAI GTCTC 5 cut(s) 38, 1184, 1300, 1622, 1841
BstMBI GATC 7 cut(s) 43, 361, 369, 1046, 1141, 1633, 2029
BstNI CCWGG 4 cut(s) 1346, 1370, 1754, 1796
BstPI GGTNACC 2 cut(s) 718, 1154
BstSCI CCNGG 8 cut(s) 847, 1241, 1344, 1368, 1537, 1752, 1794, 2038
BstSFI CTRYAG 1 cut(s) 1901
BstSLI GKGCMC 2 cut(s) 1062, 1208
BstSNI TACGTA 1 cut(s) 1221
BstUI CGCG 6 cut(s) 815, 873, 969, 1277, 1695, 1839
BstV1I GCAGC 7 cut(s) 106, 141, 237, 378, 1040, 1428, 1887
BstV2I GAAGAC 1 cut(s) 2007
BstX2I RGATCY 2 cut(s) 369, 1633
BstXI CCANNNNNNTGG 2 cut(s) 27, 1802
BstYI RGATCY 2 cut(s) 369, 1633
BstZ17I GTATAC 1 cut(s) 1396
BsuI GTATCC 1 cut(s) 2047
BsuRI GGCC 5 cut(s) 429, 450, 1060, 1229, 1361
BtgI CCRYGG 1 cut(s) 254
BtgZI GCGATG 2 cut(s) 1292, 1976
BtsCI GGATG 7 cut(s) 954, 1296, 1709, 1812, 1867, 1986, 1996
BtsI GCAGTG 2 cut(s) 584, 2032
BtsIMutI CAGTG 6 cut(s) 53, 584, 1069, 1352, 1471, 2032
Cac8I GCNNGC 8 cut(s) 60, 248, 305, 1026, 1388, 1663, 1905, 2069
CaiI CAGNNNCTG 1 cut(s) 2068
CfoI GCGC 5 cut(s) 53, 873, 1661, 1839, 1939
Cfr10I RCCGGY 2 cut(s) 378, 638
Cfr13I GGNCC 8 cut(s) 641, 851, 922, 1058, 1059, 1348, 1359, 1825
CseI GACGC 5 cut(s) 735, 1170, 1201, 1283, 1732
CsiI ACCWGGT 1 cut(s) 1344
Csp6I GTAC 4 cut(s) 1218, 1222, 1282, 1732
CspAI ACCGGT 1 cut(s) 638
CviAII CATG 5 cut(s) 255, 1436, 1482, 1550, 1802
CviQI GTAC 4 cut(s) 1218, 1222, 1282, 1732
DdeI CTNAG 9 cut(s) 126, 401, 590, 1020, 1331, 1355, 1374, 1497, 1976
DpnI GATC 7 cut(s) 45, 363, 371, 1048, 1143, 1635, 2031
DpnII GATC 7 cut(s) 43, 361, 369, 1046, 1141, 1633, 2029
DraI TTTAAA 1 cut(s) 114
DriI GACNNNNNGTC 1 cut(s) 1482
EaeI YGGCCR 1 cut(s) 427
Eam1104I CTCTTC 3 cut(s) 476, 514, 1839
Eam1105I GACNNNNNGTC 1 cut(s) 1482
EarI CTCTTC 3 cut(s) 476, 514, 1839
EciI GGCGGA 3 cut(s) 587, 810, 1202
Ecl136II GAGCTC 2 cut(s) 58, 1759
Eco105I TACGTA 1 cut(s) 1221
Eco130I CCWWGG 2 cut(s) 254, 1817
Eco24I GRGCYC 3 cut(s) 60, 1062, 1761
Eco31I GGTCTC 1 cut(s) 1622
Eco47I GGWCC 5 cut(s) 641, 851, 922, 1348, 1825
Eco53kI GAGCTC 2 cut(s) 58, 1759
Eco57I CTGAAG 1 cut(s) 1434
Eco72I CACGTG 2 cut(s) 1312, 2011
Eco88I CYCGRG 2 cut(s) 1285, 1873
Eco91I GGTNACC 2 cut(s) 718, 1154
EcoICRI GAGCTC 2 cut(s) 58, 1759
EcoNI CCTNNNNNAGG 1 cut(s) 1793
EcoO109I RGGNCCY 2 cut(s) 1058, 1359
EcoO65I GGTNACC 2 cut(s) 718, 1154
EcoRI GAATTC 1 cut(s) 1639
EcoRII CCWGG 4 cut(s) 1344, 1368, 1752, 1794
EcoT14I CCWWGG 2 cut(s) 254, 1817
EcoT38I GRGCYC 3 cut(s) 60, 1062, 1761
ErhI CCWWGG 2 cut(s) 254, 1817
Esp3I CGTCTC 1 cut(s) 1184
FaeI CATG 5 cut(s) 258, 1439, 1485, 1553, 1805
FalI AAGNNNNNCTT 2 cut(s) 1763, 1795
FaqI GGGAC 3 cut(s) 627, 819, 1334
FatI CATG 5 cut(s) 254, 1435, 1481, 1549, 1801
FauI CCCGC 8 cut(s) 770, 794, 866, 902, 971, 995, 1347, 1451
FbaI TGATCA 2 cut(s) 43, 2029
FblI GTMKAC 1 cut(s) 1395
FokI GGATG 7 cut(s) 961, 1303, 1696, 1799, 1874, 1993, 2003
FriOI GRGCYC 3 cut(s) 60, 1062, 1761
FspBI CTAG 4 cut(s) 1322, 1656, 1818, 1995
FspI TGCGCA 1 cut(s) 52
GlaI GCGC 5 cut(s) 52, 872, 1660, 1838, 1938
GsaI CCCAGC 2 cut(s) 101, 1199
GsuI CTGGAG 2 cut(s) 1184, 1775
HaeIII GGCC 5 cut(s) 429, 450, 1060, 1229, 1361
HgaI GACGC 5 cut(s) 735, 1170, 1201, 1283, 1732
HhaI GCGC 5 cut(s) 53, 873, 1661, 1839, 1939
Hin1I GRCGYC 3 cut(s) 1181, 1478, 1743
Hin1II CATG 5 cut(s) 258, 1439, 1485, 1553, 1805
Hin6I GCGC 5 cut(s) 51, 871, 1659, 1837, 1937
HinP1I GCGC 5 cut(s) 51, 871, 1659, 1837, 1937
HincII GTYRAC 2 cut(s) 216, 845
HindII GTYRAC 2 cut(s) 216, 845
HindIII AAGCTT 1 cut(s) 323
HpaI GTTAAC 1 cut(s) 216
HphI GGTGA 6 cut(s) 628, 730, 1088, 1115, 1270, 1935
Hpy166II GTNNAC 7 cut(s) 216, 636, 845, 915, 1315, 1396, 1732
Hpy188III TCNNGA 7 cut(s) 319, 373, 705, 1163, 1252, 1443, 1452
Hpy8I GTNNAC 7 cut(s) 216, 636, 845, 915, 1315, 1396, 1732
Hpy99I CGWCG 5 cut(s) 545, 751, 754, 829, 1186
HpyAV CCTTC 5 cut(s) 281, 289, 1094, 1264, 1789
HpyCH4III ACNGT 6 cut(s) 24, 798, 919, 1160, 1258, 2059
HpyCH4V TGCA 4 cut(s) 78, 622, 784, 1903
HpyF3I CTNAG 9 cut(s) 126, 401, 590, 1020, 1331, 1355, 1374, 1497, 1976
Hsp92I GRCGYC 3 cut(s) 1181, 1478, 1743
Hsp92II CATG 5 cut(s) 258, 1439, 1485, 1553, 1805
HspAI GCGC 5 cut(s) 51, 871, 1659, 1837, 1937
Kpn2I TCCGGA 1 cut(s) 704
Ksp22I TGATCA 2 cut(s) 43, 2029
KspAI GTTAAC 1 cut(s) 216
Kzo9I GATC 7 cut(s) 43, 361, 369, 1046, 1141, 1633, 2029
LmnI GCTCC 7 cut(s) 88, 600, 900, 993, 1133, 1756, 1764
Lsp1109I GCAGC 7 cut(s) 106, 141, 237, 378, 1040, 1428, 1887
LweI GCATC 3 cut(s) 190, 1895, 1981
MabI ACCWGGT 1 cut(s) 1344
MaeI CTAG 4 cut(s) 1322, 1656, 1818, 1995
MaeIII GTNAC 6 cut(s) 316, 582, 718, 1154, 1252, 2006
MalI GATC 7 cut(s) 45, 363, 371, 1048, 1143, 1635, 2031
MbiI CCGCTC 4 cut(s) 83, 655, 1830, 2000
MboI GATC 7 cut(s) 43, 361, 369, 1046, 1141, 1633, 2029
MboII GAAGA 8 cut(s) 463, 501, 670, 1661, 1853, 1856, 2007, 2090
MflI RGATCY 2 cut(s) 369, 1633
MhlI GDGCHC 5 cut(s) 60, 1062, 1208, 1684, 1761
MluCI AATT 3 cut(s) 107, 711, 1639
MlyI GAGTC 7 cut(s) 324, 405, 841, 973, 1247, 1768, 1862
MmeI TCCRAC 3 cut(s) 543, 2031, 2043
MroI TCCGGA 1 cut(s) 704
MseI TTAA 6 cut(s) 113, 215, 885, 1488, 1544, 1914
MslI CAYNNNNRTG 2 cut(s) 1687, 1922
MspA1I CMGCKG 2 cut(s) 97, 391
MspR9I CCNGG 8 cut(s) 849, 1243, 1346, 1370, 1539, 1754, 1796, 2040
Mva1269I GAATGC 1 cut(s) 1694
MvaI CCWGG 4 cut(s) 1346, 1370, 1754, 1796
MvnI CGCG 6 cut(s) 815, 873, 969, 1277, 1695, 1839
NciI CCSGG 4 cut(s) 849, 1243, 1539, 2040
NcoI CCATGG 1 cut(s) 254
NdeII GATC 7 cut(s) 43, 361, 369, 1046, 1141, 1633, 2029
NlaIII CATG 5 cut(s) 258, 1439, 1485, 1553, 1805
NlaIV GGNNCC 9 cut(s) 596, 643, 852, 1059, 1060, 1337, 1350, 1585, 1826
NmuCI GTSAC 5 cut(s) 316, 582, 718, 1252, 2006
NsbI TGCGCA 1 cut(s) 52
NspV TTCGAA 1 cut(s) 341
PcsI WCGNNNNNNNCGW 1 cut(s) 900
PctI GAATGC 1 cut(s) 1694
PfeI GAWTC 3 cut(s) 138, 733, 1595
PflFI GACNNNGTC 2 cut(s) 1790, 1852
PfoI TCCNGGA 2 cut(s) 1752, 1794
PinAI ACCGGT 1 cut(s) 638
PleI GAGTC 7 cut(s) 323, 404, 840, 972, 1246, 1768, 1861
PmaCI CACGTG 2 cut(s) 1312, 2011
PmlI CACGTG 2 cut(s) 1312, 2011
PpsI GAGTC 7 cut(s) 323, 404, 840, 972, 1246, 1768, 1861
Ppu21I YACGTR 3 cut(s) 1221, 1312, 2011
Psp124BI GAGCTC 2 cut(s) 60, 1761
Psp6I CCWGG 4 cut(s) 1344, 1368, 1752, 1794
PspCI CACGTG 2 cut(s) 1312, 2011
PspEI GGTNACC 2 cut(s) 718, 1154
PspFI CCCAGC 2 cut(s) 97, 1195
PspGI CCWGG 4 cut(s) 1344, 1368, 1752, 1794
PspN4I GGNNCC 9 cut(s) 596, 643, 852, 1059, 1060, 1337, 1350, 1585, 1826
PspOMI GGGCCC 1 cut(s) 1058
PspPI GGNCC 8 cut(s) 641, 851, 922, 1058, 1059, 1348, 1359, 1825
PstI CTGCAG 1 cut(s) 1905
PstNI CAGNNNCTG 1 cut(s) 2068
PsuI RGATCY 2 cut(s) 369, 1633
PsyI GACNNNGTC 2 cut(s) 1790, 1852
PvuII CAGCTG 2 cut(s) 97, 391
RsaI GTAC 4 cut(s) 1219, 1223, 1283, 1733
RsaNI GTAC 4 cut(s) 1218, 1222, 1282, 1732
RseI CAYNNNNRTG 2 cut(s) 1687, 1922
SacI GAGCTC 2 cut(s) 60, 1761
SaqAI TTAA 6 cut(s) 113, 215, 885, 1488, 1544, 1914
Sau3AI GATC 7 cut(s) 43, 361, 369, 1046, 1141, 1633, 2029
Sau96I GGNCC 8 cut(s) 641, 851, 922, 1058, 1059, 1348, 1359, 1825
SchI GAGTC 7 cut(s) 324, 405, 841, 973, 1247, 1768, 1862
ScrFI CCNGG 8 cut(s) 849, 1243, 1346, 1370, 1539, 1754, 1796, 2040
SduI GDGCHC 5 cut(s) 60, 1062, 1208, 1684, 1761
SexAI ACCWGGT 1 cut(s) 1344
SfaNI GCATC 3 cut(s) 190, 1895, 1981
SfcI CTRYAG 1 cut(s) 1901
SfuI TTCGAA 1 cut(s) 341
SinI GGWCC 5 cut(s) 641, 851, 922, 1348, 1825
SmiMI CAYNNNNRTG 2 cut(s) 1687, 1922
SmlI CTYRAG 2 cut(s) 1175, 2086
SmoI CTYRAG 2 cut(s) 1175, 2086
SnaBI TACGTA 1 cut(s) 1221
Sse9I AATT 3 cut(s) 107, 711, 1639
SspI AATATT 1 cut(s) 338
SspMI CTAG 4 cut(s) 1322, 1656, 1818, 1995
SstI GAGCTC 2 cut(s) 60, 1761
StyD4I CCNGG 8 cut(s) 847, 1241, 1344, 1368, 1537, 1752, 1794, 2038
StyI CCWWGG 2 cut(s) 254, 1817
TaaI ACNGT 6 cut(s) 24, 798, 919, 1160, 1258, 2059
TaqI TCGA 7 cut(s) 136, 234, 341, 384, 689, 827, 1647
TasI AATT 3 cut(s) 107, 711, 1639
TatI WGTACW 1 cut(s) 1731
TauI GCSGC 5 cut(s) 262, 603, 972, 1468, 1695
TfiI GAWTC 3 cut(s) 138, 733, 1595
Tru1I TTAA 6 cut(s) 113, 215, 885, 1488, 1544, 1914
Tru9I TTAA 6 cut(s) 113, 215, 885, 1488, 1544, 1914
TscAI CASTG 6 cut(s) 53, 591, 1069, 1359, 1478, 2032
TseFI GTSAC 5 cut(s) 316, 582, 718, 1252, 2006
TseI GCWGC 7 cut(s) 94, 154, 250, 391, 1028, 1416, 1900
Tsp45I GTSAC 5 cut(s) 316, 582, 718, 1252, 2006
TspDTI ATGAA 1 cut(s) 1424
TspGWI ACGGA 1 cut(s) 1622
TspRI CASTG 6 cut(s) 53, 591, 1069, 1359, 1478, 2032
Tth111I GACNNNGTC 2 cut(s) 1790, 1852
VpaK11BI GGWCC 5 cut(s) 641, 851, 922, 1348, 1825
XagI CCTNNNNNAGG 1 cut(s) 1793
XapI RAATTY 3 cut(s) 107, 711, 1639
XcmI CCANNNNNNNNNTGG 1 cut(s) 165
XmaJI CCTAGG 1 cut(s) 1817
XmiI GTMKAC 1 cut(s) 1395
XspI CTAG 4 cut(s) 1322, 1656, 1818, 1995
ZraI GACGTC 1 cut(s) 1479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.