pycom13g01470

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004318
Physical Location & Seq
Forward (+)
945724 .. 951180
5457 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3162 bp
ATGCAATATTATAAGAAAGTAGACAGAGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAATTAGACGACCCAAAACTCCTTTTCTACTATACATTCATTGTACTGCATCAATCCACCATCTTTGTATAATTGTAGAGATAAGATATGAGATGGGTAAAGAGGAAACAAGAAGGAAAATCAAAGAAATTGGTTTAATGCTGATGGCTAGGAAGATGAAGAGCCACCACTCGGTGGCTGTGAGGTTGAATGAGCAACTGGGGGCCAAAAAAGGCTACACCTTTGTGCAGGCTCTCAGGGCTTGGTTCCCAAAACTTTTCCTTCTTTGGGTTGGTGTGATGCTTCTCTTGGGCTGGTTAACCTACAGATACATGGATGCTGATAACAAAGTGAGAAGGGTAGAACTTCTTGGTAGCATGGCAAGAATGCTGCAGGATCAGTTCAGTGTTAGTGTTAATCATGTGCATGCCCTTGCAATCCTTGTTTCCACCTTCCATTACTACAAGAACCCTTCAGCAATTGATCAGGAAACATTTGCTGAATACACTGCCAGAACCGCCTTCGAGAGGCCTCTTTTAAGTGGGGTGGCTTATGCACAAAGAGTGCTTGATTCGGATAGGGAAAAGTTTGAAAACCAACATGGTTGGACAATAAAGACGATGGAGAGGGAACCATCGCCCATCCGAGATGAGTATGCGCCTGTAATTTTTTCGCAGGAAACCGTCTCTTACATCGAGTCACTTGATATGATGTCTGGGGAGGAGGACAGAGAAAACATTTTGAGGGCCAGAGCTACTGGAAAAGCTGTTCTAACAAGTCCATTTAGGCTGCTGGGTTCTCATCATCTTGGTGTTGTATTGACTTTCCCCGTTTACAAATCGAAGCTCCCTCCAAACCCAACTGTGGAGCAGCGCATCCAGGCGGCCGTTGGGTACCTTGGTGGTGCCTTTGATGTTGAGTCCCTAGTGGAGAATTTGCTTGGACAACTTGCTGGGAATCAAGTCATTCAGGTTTATGTGTATGATGTTACAAACACTTCTGATCCCCTAATCATGTATGGTCACCAATATCAAGATGGTGACACATCTCTTCTGCATGAAAGCAAGCTGGATTTTGGAGATCCATTCCGAAGGCATCAGATGATATGTAGATATCATCAGACGGCACCTACGTCATGGACAGCGGTTAACACTGCATTCTTGTTCTTTGTGATTGGTTTATTGGTTGGCTATATCTTATATGGAGCAGCAATGCACATTGTTAAAGTTGAGGATGATTTCCGTGAAATGGAGGAACTAAAAGTTCGAGCAGAAGCTGCTGATGTTGCCAAGTCCCAGTTTCTTGCTACTGTTTCTCATGAAATAAGAACGCCCATGAACGGAATCCTAGGAATGCTTGCCTTGCTTTTAGATACAGCTTTAAGTTCGACCCAGATGGATTATGCTCGAACTGCTCAAGCTTGTGGAAAGGCGTTGATAACATTAATTAACGAGGTGCTTGACCGTGCAAAGATTGACGCTGGCAAGTTAGAGCTGGAAGAAGTTCCATTTGGTATTCGGTCCATACTAGATGATGTCCTGTCTTTATTTTCTGAAAAAACTAGAAATAAGGGTATAGAGCTGGCAGTATTTGTTTCCGATAAAGTCCCAGAAATTTTTATGGGAGATCCAGGGAGATTCAGACAGATAATTACAAATCTAGTGGGTAACTCTATTAAATTCACTGAACGAGGACATATATTTGTCAAAGTCCATCTAGCTGAGCCCTCAAAGGTGATGATAAATGGGAAGTCAGAGACTTACTTGAATGGAGGACCAGATGAAGGTGTGCTGACATCCGATGGCCATCAGTTTAAAACTTTAAGTGGGTGTGAAGCAGCTAATGACTGGAATAGTTGGGATATGTTTAAGCATCTGGTATCTGATGAAGAATACAGAGCTGATGTTGATGAAGCTTCTGAGAAGGTCACGTTGATGGTTTCTGTGGAGGATACGGGGATTGGTATACCATTAGGTGCGCAGGAACGTGTTTTTATGCCCTTCATGCAGGCAGACAGTTCAACTTCTAGACATTATGGAGGAACTGGTATTGGCTTGAGCATCAGTAAGTGTCTGGTTGAACTGATGGGTGGTCAGATAAAATTCGTAAGTCGACCACATGTTGGGAGTACATTTTCTTTCACTGCTAATTTTAGGAGGTGCCAGAAAAACGCATTTAGTGACATGAAAAAACCTAATCCTGAGGATCTACCTTCTAGTTTGAGAGGATTGCGAGCAATAGTAGTTGATAGAAAGCTTGTGAGAGCTACTGTAACCAAATACCACTTGAAGAGACTTGGAATCCTTGTGGAAGTTGCGAGCAGCATCAAGATGGCCGTTGCTTCATGTGGAAGAAATGGTTCTGTGGCATCCGGGAATAGTATCCAGCCAGATATAGTTCTAGTTGAGAAGGATTCATGGATTTCTGGTGAAGAACATGATCTTAATGCGCAGAAATTGGACTGGAAACAGAACGGTCATGCGTTTAAGCTGCCAAAAATGATTCTTCTTGCAACCAAAAATCTTAGTCAAGCTGAATCTGATAATGTAAGGGCAGCAGGTTTTGCGGATACTGTAATCATGAAACCTTTGAGGGCTAGTATGGTGGCTGCGTGTCTTCAAGAGGTCCTGGGCATAGGAAAGAAGGGGCAACCAGGGAGAGTGGTGCTGCATGGATCTAACGTTCTCCAAAGTCTGCTCTGTGGGAAGAAAATTTTGGTGGTTGATGACAATTTGGTAAACAGGAGAGTTGCTGCAGGTGCACTAAAGAAGTTTGGAGCTAATGTAGAGTGTGTTGATAGTGGCAAAGCTGCATTGGCGTTGCTTCAATTACCGCACAATTTTGATGCTTGCTTCATGGATATTCAAATGCCTGAAATGGATGGGTTTGAGGCAACTCGCAGAATCCGGCAGATGGAGAGCAAGGCAAATGTTGAGATGATTGGAGGATTTGAAGGACTAACAAGTAAGGGTGACTGGCATTTGCCTGTATTAGCTATGACGGCGGATGTTATTCATGCCACCTATGATGAGTGCCTGAAGTGTGGGATGGATGGGTACGTCTCAAAGCCCTTCGAGGAAGAGAATCTCTATCAAGCAGTGGCCAAGTTCTTCAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004721 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006464 GO:0006468 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0007275 GO:0008150 GO:0008152 GO:0008272 GO:0009267 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009743 GO:0009744 GO:0009755 GO:0009784 GO:0009790 GO:0009884 GO:0009885 GO:0009987 GO:0009991 GO:0010015 GO:0010029 GO:0010033 GO:0010035 GO:0010086 GO:0012505 GO:0015698 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016787 GO:0016788 GO:0016791 GO:0018106 GO:0018193 GO:0018202 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0022622 GO:0023052 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032870 GO:0033500 GO:0033554 GO:0034285 GO:0035556 GO:0036211 GO:0038023 GO:0042221 GO:0042578 GO:0042592 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048509 GO:0048580 GO:0048598 GO:0048731 GO:0048831 GO:0048856 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065008 GO:0070887 GO:0071214 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071470 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0072348 GO:0098542 GO:0099402 GO:0104004 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1905392 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1054

Amino Acids

117.88

Weight (kDa)

7.02

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 173 - 353 1e-38 CHASE domain
HisKA PF00512 443 - 508 8.1e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 654 - 730 4.2e-17 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 751 - 891 2.8e-52 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 918 - 996 1.3e-15 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 12
AarI CACCTGC 1 cut(s) 2786
Acc16I TGCGCA 2 cut(s) 2019, 2490
Acc36I ACCTGC 2 cut(s) 2588, 2786
Acc65I GGTACC 1 cut(s) 933
AccB1I GGYRCC 4 cut(s) 933, 944, 1165, 2199
AccB7I CCANNNNNTGG 2 cut(s) 235, 2162
AccI GTMKAC 3 cut(s) 21, 2005, 2152
AciI CCGC 6 cut(s) 558, 923, 1184, 2606, 2873, 3044
AclI AACGTT 1 cut(s) 2721
AclWI GGATC 6 cut(s) 444, 1037, 1115, 1661, 2253, 2722
AcoI YGGCCR 4 cut(s) 924, 1843, 2373, 3141
AcsI RAATTY 5 cut(s) 973, 1653, 1718, 2141, 2751
AcuI CTGAAG 2 cut(s) 498, 3098
AdeI CACNNNGTG 1 cut(s) 235
AfaI GTAC 4 cut(s) 106, 935, 2170, 3098
AflIII ACRYGT 2 cut(s) 2026, 2158
AjnI CCWGG 4 cut(s) 918, 1669, 2667, 2692
AjuI GAANNNNNNNTTGG 4 cut(s) 1533, 1565, 2738, 2770
AleI CACNNNNGTG 1 cut(s) 284
AloI GAACNNNNNNTCC 4 cut(s) 792, 824, 2706, 2738
Alw21I GWGCWC 1 cut(s) 2803
Alw26I GTCTC 4 cut(s) 730, 1790, 2326, 3106
Alw44I GTGCAC 1 cut(s) 2799
AlwI GGATC 6 cut(s) 444, 1037, 1115, 1661, 2253, 2722
AlwNI CAGNNNCTG 1 cut(s) 1316
AoxI GGCC 7 cut(s) 264, 569, 786, 924, 1843, 2373, 3141
ApaLI GTGCAC 1 cut(s) 2799
ApoI RAATTY 5 cut(s) 973, 1653, 1718, 2141, 2751
AseI ATTAAT 1 cut(s) 1484
Asp700I GAANNNNTTC 2 cut(s) 1542, 2398
Asp718I GGTACC 1 cut(s) 933
AspA2I CCTAGG 1 cut(s) 1387
AspLEI GCGC 4 cut(s) 700, 915, 2020, 2491
AspS9I GGNCC 5 cut(s) 264, 786, 1560, 1814, 2665
AsuC2I CCSGG 1 cut(s) 2413
AsuHPI GGTGA 5 cut(s) 1055, 1091, 1786, 2480, 3023
AvaII GGWCC 3 cut(s) 1560, 1814, 2665
AvrII CCTAGG 1 cut(s) 1387
AxyI CCTNAGG 1 cut(s) 2241
BaeGI GKGCMC 1 cut(s) 2803
BaeI ACNNNNGTAYC 2 cut(s) 925, 958
BalI TGGCCA 2 cut(s) 1845, 3143
BanI GGYRCC 4 cut(s) 933, 944, 1165, 2199
BanII GRGCYC 1 cut(s) 1767
BbsI GAAGAC 1 cut(s) 2648
Bbv12I GWGCWC 1 cut(s) 2803
BceAI ACGGC 4 cut(s) 911, 1179, 2360, 3057
BciT130I CCWGG 4 cut(s) 920, 1671, 2669, 2694
BciVI GTATCC 3 cut(s) 1984, 2432, 2602
BclI TGATCA 1 cut(s) 523
BcnI CCSGG 1 cut(s) 2413
BcoDI GTCTC 4 cut(s) 730, 1790, 2326, 3106
BfmI CTRYAG 3 cut(s) 364, 431, 2793
BfuAI ACCTGC 2 cut(s) 2588, 2786
BfuI GTATCC 3 cut(s) 1984, 2432, 2602
BlnI CCTAGG 1 cut(s) 1387
BlpI GCTNAGC 1 cut(s) 1761
Bme1390I CCNGG 5 cut(s) 920, 1671, 2413, 2669, 2694
Bme18I GGWCC 3 cut(s) 1560, 1814, 2665
BmgT120I GGNCC 5 cut(s) 264, 786, 1560, 1814, 2665
BmiI GGNNCC 7 cut(s) 265, 308, 672, 935, 946, 1167, 2201
BmrFI CCNGG 5 cut(s) 920, 1671, 2413, 2669, 2694
BmrI ACTGGG 2 cut(s) 269, 1330
BmuI ACTGGG 2 cut(s) 269, 1330
BpiI GAAGAC 1 cut(s) 2648
Bpu1102I GCTNAGC 1 cut(s) 1761
BpuEI CTTGAG 2 cut(s) 1440, 2116
BpuMI CCSGG 1 cut(s) 2413
BsaBI GATNNNNATC 1 cut(s) 1845
BsaJI CCNNGG 5 cut(s) 937, 1387, 1670, 2668, 2693
BsaXI ACNNNNNCTCC 2 cut(s) 2808, 2838
Bse1I ACTGG 7 cut(s) 264, 802, 1336, 1892, 2089, 2507, 3020
Bse21I CCTNAGG 1 cut(s) 2241
Bse3DI GCAATG 1 cut(s) 1257
Bse8I GATNNNNATC 1 cut(s) 1845
BseBI CCWGG 4 cut(s) 920, 1671, 2669, 2694
BseDI CCNNGG 5 cut(s) 937, 1387, 1670, 2668, 2693
BseJI GATNNNNATC 1 cut(s) 1845
BseMI GCAATG 1 cut(s) 1257
BseMII CTCAG 4 cut(s) 310, 1752, 1950, 2232
BseNI ACTGG 7 cut(s) 264, 802, 1336, 1892, 2089, 2507, 3020
BseRI GAGGAG 2 cut(s) 42, 776
BseSI GKGCMC 1 cut(s) 2803
BseX3I CGGCCG 1 cut(s) 924
BseYI CCCAGC 2 cut(s) 832, 992
BsgI GTGCAG 1 cut(s) 308
Bsh1285I CGRYCG 1 cut(s) 927
BshFI GGCC 7 cut(s) 266, 571, 788, 926, 1845, 2375, 3143
BshNI GGYRCC 4 cut(s) 933, 944, 1165, 2199
BsiEI CGRYCG 1 cut(s) 927
BsiHKAI GWGCWC 1 cut(s) 2803
BsiSI CCGG 2 cut(s) 2412, 2947
BslFI GGGAC 3 cut(s) 946, 1318, 1631
BsmAI GTCTC 4 cut(s) 730, 1790, 2326, 3106
BsmBI CGTCTC 2 cut(s) 730, 3106
BsmFI GGGAC 3 cut(s) 946, 1318, 1631
BsmI GAATGC 3 cut(s) 432, 1196, 1398
BsnI GGCC 7 cut(s) 266, 571, 788, 926, 1845, 2375, 3143
Bsp1286I GDGCHC 2 cut(s) 1767, 2803
Bsp143I GATC 8 cut(s) 436, 523, 1042, 1120, 1666, 2245, 2479, 2714
Bsp1720I GCTNAGC 1 cut(s) 1761
BspACI CCGC 6 cut(s) 558, 923, 1184, 2606, 2873, 3044
BspANI GGCC 7 cut(s) 266, 571, 788, 926, 1845, 2375, 3143
BspCNI CTCAG 4 cut(s) 309, 1753, 1951, 2233
BspHI TCATGA 2 cut(s) 1357, 2619
BspLI GGNNCC 7 cut(s) 265, 308, 672, 935, 946, 1167, 2201
BspMAI CTGCAG 2 cut(s) 435, 2797
BspMI ACCTGC 2 cut(s) 2588, 2786
BspPI GGATC 6 cut(s) 444, 1037, 1115, 1661, 2253, 2722
BspQI GCTCTTC 1 cut(s) 215
BspT107I GGYRCC 4 cut(s) 933, 944, 1165, 2199
BsrDI GCAATG 1 cut(s) 1257
BsrI ACTGG 7 cut(s) 264, 802, 1336, 1892, 2089, 2507, 3020
BssECI CCNNGG 5 cut(s) 937, 1387, 1670, 2668, 2693
BssMI GATC 8 cut(s) 436, 523, 1042, 1120, 1666, 2245, 2479, 2714
BssNAI GTATAC 1 cut(s) 2006
BssT1I CCWWGG 2 cut(s) 937, 1387
Bst1107I GTATAC 1 cut(s) 2006
Bst2UI CCWGG 4 cut(s) 920, 1671, 2669, 2694
Bst4CI ACNGT 8 cut(s) 724, 904, 1351, 1505, 2057, 2311, 2516, 2614
Bst6I CTCTTC 4 cut(s) 215, 1095, 2324, 3114
BstAPI GCANNNNNTGC 2 cut(s) 1316, 2603
BstDEI CTNAG 5 cut(s) 296, 1761, 1959, 2241, 2564
BstEII GGTNACC 1 cut(s) 1061
BstENI CCTNNNNNAGG 1 cut(s) 565
BstHHI GCGC 4 cut(s) 700, 915, 2020, 2491
BstKTI GATC 8 cut(s) 439, 526, 1045, 1123, 1669, 2248, 2482, 2717
BstMAI GTCTC 4 cut(s) 730, 1790, 2326, 3106
BstMBI GATC 8 cut(s) 436, 523, 1042, 1120, 1666, 2245, 2479, 2714
BstMCI CGRYCG 1 cut(s) 927
BstNI CCWGG 4 cut(s) 920, 1671, 2669, 2694
BstNSI RCATGY 2 cut(s) 470, 2162
BstPI GGTNACC 1 cut(s) 1061
BstSCI CCNGG 5 cut(s) 918, 1669, 2411, 2667, 2692
BstSFI CTRYAG 3 cut(s) 364, 431, 2793
BstSLI GKGCMC 1 cut(s) 2803
BstV2I GAAGAC 1 cut(s) 2648
BstX2I RGATCY 4 cut(s) 1120, 1666, 2245, 2714
BstYI RGATCY 4 cut(s) 1120, 1666, 2245, 2714
BstZ17I GTATAC 1 cut(s) 2006
BstZI CGGCCG 1 cut(s) 924
Bsu36I CCTNAGG 1 cut(s) 2241
BsuI GTATCC 3 cut(s) 1984, 2432, 2602
BsuRI GGCC 7 cut(s) 266, 571, 788, 926, 1845, 2375, 3143
BtgZI GCGATG 1 cut(s) 660
BtsI GCAGTG 4 cut(s) 546, 1191, 2181, 3144
BtsIMutI CAGTG 6 cut(s) 451, 546, 1191, 1722, 2181, 3144
BveI ACCTGC 2 cut(s) 2588, 2786
CaiI CAGNNNCTG 1 cut(s) 1316
CciI TCATGA 2 cut(s) 1357, 2619
CfoI GCGC 4 cut(s) 700, 915, 2020, 2491
Cfr13I GGNCC 5 cut(s) 264, 786, 1560, 1814, 2665
CseI GACGC 1 cut(s) 1526
Csp6I GTAC 4 cut(s) 105, 934, 2169, 3097
CspCI CAANNNNNGTGG 4 cut(s) 107, 142, 1683, 1718
CviQI GTAC 4 cut(s) 105, 934, 2169, 3097
DdeI CTNAG 5 cut(s) 296, 1761, 1959, 2241, 2564
DpnI GATC 8 cut(s) 438, 525, 1044, 1122, 1668, 2247, 2481, 2716
DpnII GATC 8 cut(s) 436, 523, 1042, 1120, 1666, 2245, 2479, 2714
DraI TTTAAA 1 cut(s) 1855
DraIII CACNNNGTG 1 cut(s) 235
EaeI YGGCCR 4 cut(s) 924, 1843, 2373, 3141
EagI CGGCCG 1 cut(s) 924
Eam1104I CTCTTC 4 cut(s) 215, 1095, 2324, 3114
EarI CTCTTC 4 cut(s) 215, 1095, 2324, 3114
EciI GGCGGA 1 cut(s) 3059
EclXI CGGCCG 1 cut(s) 924
Eco130I CCWWGG 2 cut(s) 937, 1387
Eco147I AGGCCT 1 cut(s) 571
Eco24I GRGCYC 1 cut(s) 1767
Eco32I GATATC 1 cut(s) 1154
Eco47I GGWCC 3 cut(s) 1560, 1814, 2665
Eco52I CGGCCG 1 cut(s) 924
Eco57I CTGAAG 2 cut(s) 498, 3098
Eco81I CCTNAGG 1 cut(s) 2241
Eco91I GGTNACC 1 cut(s) 1061
EcoNI CCTNNNNNAGG 1 cut(s) 565
EcoO109I RGGNCCY 1 cut(s) 2665
EcoO65I GGTNACC 1 cut(s) 1061
EcoRII CCWGG 4 cut(s) 918, 1669, 2667, 2692
EcoRV GATATC 1 cut(s) 1154
EcoT14I CCWWGG 2 cut(s) 937, 1387
EcoT38I GRGCYC 1 cut(s) 1767
ErhI CCWWGG 2 cut(s) 937, 1387
Esp3I CGTCTC 2 cut(s) 730, 3106
FaqI GGGAC 3 cut(s) 946, 1318, 1631
FbaI TGATCA 1 cut(s) 523
FblI GTMKAC 3 cut(s) 21, 2005, 2152
FriOI GRGCYC 1 cut(s) 1767
FspI TGCGCA 2 cut(s) 2019, 2490
GlaI GCGC 4 cut(s) 699, 914, 2019, 2490
GsaI CCCAGC 2 cut(s) 836, 996
HaeIII GGCC 7 cut(s) 266, 571, 788, 926, 1845, 2375, 3143
HapII CCGG 2 cut(s) 2412, 2947
HgaI GACGC 1 cut(s) 1526
HhaI GCGC 4 cut(s) 700, 915, 2020, 2491
Hin6I GCGC 4 cut(s) 698, 913, 2018, 2489
HinP1I GCGC 4 cut(s) 698, 913, 2018, 2489
HincII GTYRAC 3 cut(s) 360, 1189, 2153
HindII GTYRAC 3 cut(s) 360, 1189, 2153
HindIII AAGCTT 3 cut(s) 1458, 1953, 2294
HpaI GTTAAC 2 cut(s) 360, 1189
HpaII CCGG 2 cut(s) 2412, 2947
HphI GGTGA 5 cut(s) 1055, 1091, 1786, 2480, 3023
Hpy166II GTNNAC 8 cut(s) 22, 360, 874, 1189, 2006, 2153, 2779, 2801
Hpy188III TCNNGA 9 cut(s) 527, 565, 1073, 1358, 2067, 2240, 2368, 2620, 2660
Hpy8I GTNNAC 8 cut(s) 22, 360, 874, 1189, 2006, 2153, 2779, 2801
HpyCH4III ACNGT 8 cut(s) 724, 904, 1351, 1505, 2057, 2311, 2516, 2614
HpyCH4IV ACGT 5 cut(s) 1172, 1970, 2026, 2721, 3099
HpyF3I CTNAG 5 cut(s) 296, 1761, 1959, 2241, 2564
HpySE526I ACGT 5 cut(s) 1172, 1970, 2026, 2721, 3099
HspAI GCGC 4 cut(s) 698, 913, 2018, 2489
KpnI GGTACC 1 cut(s) 937
Ksp22I TGATCA 1 cut(s) 523
KspAI GTTAAC 2 cut(s) 360, 1189
Kzo9I GATC 8 cut(s) 436, 523, 1042, 1120, 1666, 2245, 2479, 2714
LguI GCTCTTC 1 cut(s) 215
LmnI GCTCC 4 cut(s) 891, 907, 1244, 2816
MaeII ACGT 5 cut(s) 1172, 1970, 2026, 2721, 3099
MaeIII GTNAC 9 cut(s) 738, 1027, 1061, 1079, 1706, 1966, 2219, 2311, 3011
MalI GATC 8 cut(s) 438, 525, 1044, 1122, 1668, 2247, 2481, 2716
MboI GATC 8 cut(s) 436, 523, 1042, 1120, 1666, 2245, 2479, 2714
MfeI CAATTG 1 cut(s) 519
MflI RGATCY 4 cut(s) 1120, 1666, 2245, 2714
MhlI GDGCHC 2 cut(s) 1767, 2803
MlsI TGGCCA 2 cut(s) 1845, 3143
MluNI TGGCCA 2 cut(s) 1845, 3143
MlyI GAGTC 2 cut(s) 746, 968
MmeI TCCRAC 1 cut(s) 626
Mox20I TGGCCA 2 cut(s) 1845, 3143
MroXI GAANNNNTTC 2 cut(s) 1542, 2398
MscI TGGCCA 2 cut(s) 1845, 3143
MslI CAYNNNNRTG 5 cut(s) 284, 465, 849, 1973, 2369
Msp20I TGGCCA 2 cut(s) 1845, 3143
MspA1I CMGCKG 1 cut(s) 1184
MspI CCGG 2 cut(s) 2412, 2947
MspR9I CCNGG 5 cut(s) 920, 1671, 2413, 2669, 2694
MunI CAATTG 1 cut(s) 519
Mva1269I GAATGC 3 cut(s) 432, 1196, 1398
MvaI CCWGG 4 cut(s) 920, 1671, 2669, 2694
NciI CCSGG 1 cut(s) 2413
NdeII GATC 8 cut(s) 436, 523, 1042, 1120, 1666, 2245, 2479, 2714
NlaIV GGNNCC 7 cut(s) 265, 308, 672, 935, 946, 1167, 2201
NmuCI GTSAC 6 cut(s) 738, 1061, 1079, 1966, 2219, 3011
NsbI TGCGCA 2 cut(s) 2019, 2490
NspI RCATGY 2 cut(s) 470, 2162
OliI CACNNNNGTG 1 cut(s) 284
PacI TTAATTAA 1 cut(s) 1488
PaeI GCATGC 1 cut(s) 470
PagI TCATGA 2 cut(s) 1357, 2619
PaqCI CACCTGC 1 cut(s) 2786
PceI AGGCCT 1 cut(s) 571
PciI ACATGT 1 cut(s) 2158
PciSI GCTCTTC 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 1169
PctI GAATGC 3 cut(s) 432, 1196, 1398
PdmI GAANNNNTTC 2 cut(s) 1542, 2398
PflMI CCANNNNNTGG 2 cut(s) 235, 2162
PfoI TCCNGGA 1 cut(s) 2411
PleI GAGTC 2 cut(s) 745, 967
PpsI GAGTC 2 cut(s) 745, 967
PpuMI RGGWCCY 1 cut(s) 2665
PscI ACATGT 1 cut(s) 2158
PshBI ATTAAT 1 cut(s) 1484
PsiI TTATAA 1 cut(s) 12
Psp1406I AACGTT 1 cut(s) 2721
Psp5II RGGWCCY 1 cut(s) 2665
Psp6I CCWGG 4 cut(s) 918, 1669, 2667, 2692
PspEI GGTNACC 1 cut(s) 1061
PspFI CCCAGC 2 cut(s) 832, 992
PspGI CCWGG 4 cut(s) 918, 1669, 2667, 2692
PspN4I GGNNCC 7 cut(s) 265, 308, 672, 935, 946, 1167, 2201
PspPI GGNCC 5 cut(s) 264, 786, 1560, 1814, 2665
PspPPI RGGWCCY 1 cut(s) 2665
PstI CTGCAG 2 cut(s) 435, 2797
PstNI CAGNNNCTG 1 cut(s) 1316
PsuI RGATCY 4 cut(s) 1120, 1666, 2245, 2714
RsaI GTAC 4 cut(s) 106, 935, 2170, 3098
RsaNI GTAC 4 cut(s) 105, 934, 2169, 3097
RseI CAYNNNNRTG 5 cut(s) 284, 465, 849, 1973, 2369
SalI GTCGAC 1 cut(s) 2151
SapI GCTCTTC 1 cut(s) 215
Sau3AI GATC 8 cut(s) 436, 523, 1042, 1120, 1666, 2245, 2479, 2714
Sau96I GGNCC 5 cut(s) 264, 786, 1560, 1814, 2665
SchI GAGTC 2 cut(s) 746, 968
ScrFI CCNGG 5 cut(s) 920, 1671, 2413, 2669, 2694
SduI GDGCHC 2 cut(s) 1767, 2803
SfcI CTRYAG 3 cut(s) 364, 431, 2793
SinI GGWCC 3 cut(s) 1560, 1814, 2665
SmiMI CAYNNNNRTG 5 cut(s) 284, 465, 849, 1973, 2369
SmlI CTYRAG 2 cut(s) 1455, 2095
SmoI CTYRAG 2 cut(s) 1455, 2095
SphI GCATGC 1 cut(s) 470
SseBI AGGCCT 1 cut(s) 571
SsiI CCGC 6 cut(s) 558, 923, 1184, 2606, 2873, 3044
SspI AATATT 1 cut(s) 8
StuI AGGCCT 1 cut(s) 571
StyD4I CCNGG 5 cut(s) 918, 1669, 2411, 2667, 2692
StyI CCWWGG 2 cut(s) 937, 1387
TaaI ACNGT 8 cut(s) 724, 904, 1351, 1505, 2057, 2311, 2516, 2614
TaiI ACGT 5 cut(s) 1175, 1973, 2029, 2724, 3102
TaqI TCGA 8 cut(s) 564, 735, 881, 1306, 1427, 1447, 2152, 3114
TaqII GACCGA 1 cut(s) 1548
TatI WGTACW 2 cut(s) 104, 2168
TauI GCSGC 1 cut(s) 926
TscAI CASTG 6 cut(s) 451, 553, 1198, 1729, 2188, 3144
TseFI GTSAC 6 cut(s) 738, 1061, 1079, 1966, 2219, 3011
Tsp45I GTSAC 6 cut(s) 738, 1061, 1079, 1966, 2219, 3011
TspGWI ACGGA 2 cut(s) 1271, 1395
TspRI CASTG 6 cut(s) 451, 553, 1198, 1729, 2188, 3144
Van91I CCANNNNNTGG 2 cut(s) 235, 2162
VneI GTGCAC 1 cut(s) 2799
VpaK11BI GGWCC 3 cut(s) 1560, 1814, 2665
VspI ATTAAT 1 cut(s) 1484
XagI CCTNNNNNAGG 1 cut(s) 565
XapI RAATTY 5 cut(s) 973, 1653, 1718, 2141, 2751
XbaI TCTAGA 1 cut(s) 2066
XceI RCATGY 2 cut(s) 470, 2162
XcmI CCANNNNNNNNNTGG 2 cut(s) 926, 1073
XmaJI CCTAGG 1 cut(s) 1387
XmiI GTMKAC 3 cut(s) 21, 2005, 2152
XmnI GAANNNNTTC 2 cut(s) 1542, 2398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.