pycom13g05840

Hemimethylated DNA-binding protein YccV like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0008100
Physical Location & Seq
Forward (+)
3825557 .. 3826277
721 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g05840.1

Sequence Viewer

Length: 333 bp
ATGGATCCGGTGTGTTCTGAGTCAAGTTCATGGATGGAAACTGCACAGGTTGAAAAGCTGACACGCGGTTCTAATCAGCCATTTTATCAGATTTTGATCGATGTACATGCTGACCCCAACCTTCTGGTGGCATATGTTTCTGAGGAGAATCTTTTAGCCCCTGAAGAACCAGATGTGGATCGATTTGATCAACCCTACATTTCATTTTTGTTCTACGGGATGGATGCAGCTGGCGATTTCATCCCCATCAGGCAATTGCGAGAGAAGTACAATAGGCCACGACACGAAATCCCCTATGATCCACTGGATGAGGGGCAAGGAAGTGATGTTTAG

Protein Analysis

111

Amino Acids

12.64

Weight (kDa)

4.21

Isoelectric Point (pI)

49.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YccV-like PF08755 1 - 76 5.8e-16 Hemimethylated DNA-binding protein YccV like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 66
AciI CCGC 1 cut(s) 66
AclWI GGATC 3 cut(s) 12, 186, 293
AcuI CTGAAG 1 cut(s) 183
AfaI GTAC 2 cut(s) 105, 269
AfiI CCNNNNNNNGG 1 cut(s) 127
AgsI TTSAA 1 cut(s) 53
AluBI AGCT 2 cut(s) 58, 230
AluI AGCT 2 cut(s) 58, 230
AlwI GGATC 3 cut(s) 12, 186, 293
AoxI GGCC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 227
BamHI GGATCC 1 cut(s) 4
BbvI GCAGC 1 cut(s) 239
BccI CCATC 3 cut(s) 28, 214, 254
BcgI CGANNNNNNTGC 2 cut(s) 89, 123
BclI TGATCA 1 cut(s) 187
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 1 cut(s) 214
Bsa29I ATCGAT 2 cut(s) 99, 181
BsaBI GATNNNNATC 2 cut(s) 95, 177
BsaWI WCCGGW 1 cut(s) 7
Bsc4I CCNNNNNNNGG 1 cut(s) 127
Bse1I ACTGG 1 cut(s) 309
Bse8I GATNNNNATC 2 cut(s) 95, 177
BseCI ATCGAT 2 cut(s) 99, 181
BseGI GGATG 5 cut(s) 39, 225, 229, 240, 313
BseJI GATNNNNATC 2 cut(s) 95, 177
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMII CTCAG 2 cut(s) 9, 132
BseNI ACTGG 1 cut(s) 309
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 1 cut(s) 239
BsgI GTGCAG 1 cut(s) 27
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 1 cut(s) 277
BshVI ATCGAT 2 cut(s) 99, 181
BsiSI CCGG 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 127
BsnI GGCC 1 cut(s) 277
Bsp1407I TGTACA 1 cut(s) 103
Bsp143I GATC 5 cut(s) 4, 96, 178, 187, 298
BspACI CCGC 1 cut(s) 66
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 2 cut(s) 10, 133
BspDI ATCGAT 2 cut(s) 99, 181
BspFNI CGCG 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 3 cut(s) 12, 186, 293
BsrGI TGTACA 1 cut(s) 103
BsrI ACTGG 1 cut(s) 309
BssMI GATC 5 cut(s) 4, 96, 178, 187, 298
BstAUI TGTACA 1 cut(s) 103
BstC8I GCNNGC 1 cut(s) 232
BstDEI CTNAG 2 cut(s) 18, 141
BstF5I GGATG 5 cut(s) 39, 225, 229, 240, 313
BstFNI CGCG 1 cut(s) 66
BstKTI GATC 5 cut(s) 7, 99, 181, 190, 301
BstMBI GATC 5 cut(s) 4, 96, 178, 187, 298
BstNSI RCATGY 1 cut(s) 110
BstUI CGCG 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 239
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 124
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 2 cut(s) 99, 181
BsuRI GGCC 1 cut(s) 277
BsuTUI ATCGAT 2 cut(s) 99, 181
BtsCI GGATG 5 cut(s) 39, 225, 229, 240, 313
BtsIMutI CAGTG 1 cut(s) 302
Cac8I GCNNGC 1 cut(s) 232
ClaI ATCGAT 2 cut(s) 99, 181
Csp6I GTAC 2 cut(s) 104, 268
CviAII CATG 2 cut(s) 30, 107
CviJI RGCY 5 cut(s) 58, 79, 158, 230, 277
CviKI_1 RGCY 5 cut(s) 58, 79, 158, 230, 277
CviQI GTAC 2 cut(s) 104, 268
DdeI CTNAG 2 cut(s) 18, 141
DpnI GATC 5 cut(s) 6, 98, 180, 189, 300
DpnII GATC 5 cut(s) 4, 96, 178, 187, 298
Eco57I CTGAAG 1 cut(s) 183
FaeI CATG 2 cut(s) 33, 110
FaiI YATR 5 cut(s) 31, 108, 133, 135, 297
FatI CATG 2 cut(s) 29, 106
FauNDI CATATG 1 cut(s) 133
FbaI TGATCA 1 cut(s) 187
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 5 cut(s) 46, 227, 232, 236, 320
Fsp4HI GCNGC 1 cut(s) 228
GluI GCNGC 1 cut(s) 228
HaeIII GGCC 1 cut(s) 277
HapII CCGG 1 cut(s) 8
Hin1II CATG 2 cut(s) 33, 110
HinfI GANTC 2 cut(s) 20, 148
HpaII CCGG 1 cut(s) 8
Hpy188I TCNGA 3 cut(s) 19, 90, 142
HpyAV CCTTC 1 cut(s) 131
HpyCH4V TGCA 2 cut(s) 44, 227
HpyF3I CTNAG 2 cut(s) 18, 141
Hsp92II CATG 2 cut(s) 33, 110
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 5 cut(s) 4, 96, 178, 187, 298
LpnPI CCDG 8 cut(s) 21, 32, 110, 174, 183, 216, 235, 290
Lsp1109I GCAGC 1 cut(s) 239
LweI GCATC 1 cut(s) 214
MalI GATC 5 cut(s) 6, 98, 180, 189, 300
MboI GATC 5 cut(s) 4, 96, 178, 187, 298
MboII GAAGA 1 cut(s) 176
MfeI CAATTG 1 cut(s) 254
MflI RGATCY 1 cut(s) 4
MluCI AATT 1 cut(s) 254
MlyI GAGTC 1 cut(s) 29
MnlI CCTC 2 cut(s) 136, 304
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 1 cut(s) 8
MunI CAATTG 1 cut(s) 254
MvnI CGCG 1 cut(s) 66
NdeI CATATG 1 cut(s) 133
NdeII GATC 5 cut(s) 4, 96, 178, 187, 298
NlaIII CATG 2 cut(s) 33, 110
NlaIV GGNNCC 1 cut(s) 6
NspI RCATGY 1 cut(s) 110
PfeI GAWTC 1 cut(s) 148
PkrI GCNGC 1 cut(s) 229
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 6
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 2 cut(s) 105, 269
RsaNI GTAC 2 cut(s) 104, 268
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 5 cut(s) 4, 96, 178, 187, 298
SchI GAGTC 1 cut(s) 29
SetI ASST 4 cut(s) 51, 60, 123, 232
SfaNI GCATC 1 cut(s) 214
Sse9I AATT 1 cut(s) 254
SsiI CCGC 1 cut(s) 66
TaqI TCGA 2 cut(s) 99, 181
TasI AATT 1 cut(s) 254
TatI WGTACW 2 cut(s) 103, 267
TfiI GAWTC 1 cut(s) 148
TscAI CASTG 1 cut(s) 309
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 3 cut(s) 18, 192, 229
TspRI CASTG 1 cut(s) 309
XceI RCATGY 1 cut(s) 110
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.