Rw0G016830

Hemimethylated DNA-binding protein YccV like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00751
OG0008100
Physical Location & Seq
Forward (+)
546345 .. 549617
3273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G016830.1

Sequence Viewer

Length: 1002 bp
ATGGTGCAAGGTTTGTCACTGAGCACTCTGGCTACGTCGCGTAGCAGTTGTGTTTGTGGATCAATGCCTTCATGGAGGAGGCATTTTAGAGTAATGAGGAAAGACCAGATAAGTAGCGAAAGAAATTTCATGTGGCATCAGTGTGATCATAGCGTATTCGTGAAAGGTAACCCTGATATGTTGAGGCAAAGGAATGTAAGGGTGGAAGCTGGGTGGCTGTTTAAAGGGGGTGATCGGGGGTCGGATGCAATCTCAGAGCGCAGTGAGAGTGCTAATGAGGATATTTTGATATTCTTCTTCCAGCTGGACCTGGCAACTCGAGTCCAGTATGCATTGAACATGGAAGAGTATGACATTGCACAACAACTGAGAACCAAGCTGGCTGAGGTTGAAGCAGAAGTTGCTAGGCAGCAGGAGGGGAAAAGGGGATCGTCTTCAAAGAGTGAAGCTCAAGATAAGGCTATAAGTCTCATTCGCTTACGGGCAGACCTGCAGGGTGCAATTGAGAGTGAGAATTATGCATTGGCAACCAAGCTACGGGATGAAATTTCCAAACTGGAGGCAGAGTCATTGGCTATATCAGCCAAAGCTTTGGCACATGAAAATGCAAACTATTCATTTCGTTTGGGACAGAGAGTGAGGCATAAAATTTTTGGCTATAGGGCTGTGGTTTGTGGAATGGATCCGGTGTGCTGTGAATCAAGTTCATGGATGGAAACTGCTCAAGTTGAAAAGTTGTCTCGGGGTTCTAATCAGCCATTTTATCAGGTTTTGGTTGATGTTCATGCAGATCCCAACCTTCTGGTGGCTTATGTTGCTGAGGAGAATCTATTGGCCCCTGACAAACCAGACTTGGATCGATTTGATCATCCCTATATTTCCTTCTTATTCTATGGGATGGATGCAGCTGGTGATTTCATCCCTATCAAGCAACTACGCGAGAAGTACAACAGGCCGCGACATGAAATACCGCTCGATGAGGAGCCTGGACGCGATGCTTAG

Protein Analysis

333

Amino Acids

37.85

Weight (kDa)

5.84

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YccV-like PF08755 206 - 302 2.6e-28 Hemimethylated DNA-binding protein YccV like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 498
AccBSI CCGCTC 1 cut(s) 973
AccII CGCG 4 cut(s) 40, 939, 958, 993
AciI CCGC 2 cut(s) 956, 971
AclWI GGATC 6 cut(s) 67, 436, 677, 690, 785, 864
AcsI RAATTY 3 cut(s) 124, 546, 648
AfaI GTAC 1 cut(s) 947
AfiI CCNNNNNNNGG 2 cut(s) 537, 805
AgsI TTSAA 4 cut(s) 337, 392, 438, 731
AjnI CCWGG 2 cut(s) 309, 985
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AluBI AGCT 7 cut(s) 209, 304, 379, 449, 535, 590, 908
AluI AGCT 7 cut(s) 209, 304, 379, 449, 535, 590, 908
Alw21I GWGCWC 1 cut(s) 26
Alw26I GTCTC 2 cut(s) 473, 744
AlwI GGATC 6 cut(s) 67, 436, 677, 690, 785, 864
Ama87I CYCGRG 2 cut(s) 318, 741
AoxI GGCC 2 cut(s) 834, 953
ApeKI GCWGC 2 cut(s) 409, 905
ApoI RAATTY 3 cut(s) 124, 546, 648
AspLEI GCGC 1 cut(s) 261
AspS9I GGNCC 2 cut(s) 307, 835
AsuHPI GGTGA 2 cut(s) 242, 923
AvaI CYCGRG 2 cut(s) 318, 741
AvaII GGWCC 1 cut(s) 307
BamHI GGATCC 1 cut(s) 682
BbsI GAAGAC 1 cut(s) 426
Bbv12I GWGCWC 1 cut(s) 26
BbvCI CCTCAGC 2 cut(s) 384, 819
BbvI GCAGC 2 cut(s) 421, 917
BccI CCATC 2 cut(s) 706, 892
BciT130I CCWGG 2 cut(s) 311, 987
BclI TGATCA 2 cut(s) 145, 865
BcoDI GTCTC 2 cut(s) 473, 744
BfaI CTAG 1 cut(s) 405
BfmI CTRYAG 2 cut(s) 491, 658
BfuAI ACCTGC 1 cut(s) 498
BisI GCNGC 3 cut(s) 410, 906, 956
BlsI GCNGC 3 cut(s) 411, 907, 957
Bme1390I CCNGG 2 cut(s) 311, 987
Bme18I GGWCC 1 cut(s) 307
BmeT110I CYCGRG 2 cut(s) 318, 741
BmgT120I GGNCC 2 cut(s) 307, 835
BmiI GGNNCC 3 cut(s) 684, 837, 984
BmrFI CCNGG 2 cut(s) 311, 987
BmsI GCATC 4 cut(s) 145, 235, 892, 985
BpiI GAAGAC 1 cut(s) 426
BplI GAGNNNNNCTC 2 cut(s) 433, 465
BpmI CTGGAG 1 cut(s) 578
Bpu10I CCTNAGC 2 cut(s) 384, 819
BpuEI CTTGAG 2 cut(s) 435, 708
Bsa29I ATCGAT 1 cut(s) 859
BsaWI WCCGGW 1 cut(s) 685
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bsc4I CCNNNNNNNGG 2 cut(s) 537, 805
Bse1I ACTGG 2 cut(s) 325, 561
Bse3DI GCAATG 1 cut(s) 354
BseBI CCWGG 2 cut(s) 311, 987
BseCI ATCGAT 1 cut(s) 859
BseGI GGATG 7 cut(s) 250, 547, 717, 868, 903, 907, 918
BseLI CCNNNNNNNGG 2 cut(s) 537, 805
BseMI GCAATG 1 cut(s) 354
BseMII CTCAG 5 cut(s) 11, 267, 359, 375, 810
BseNI ACTGG 2 cut(s) 325, 561
BseRI GAGGAG 3 cut(s) 91, 836, 995
BseXI GCAGC 2 cut(s) 421, 917
BseYI CCCAGC 1 cut(s) 209
Bsh1236I CGCG 4 cut(s) 40, 939, 958, 993
BshFI GGCC 2 cut(s) 836, 955
BshVI ATCGAT 1 cut(s) 859
BsiHKAI GWGCWC 1 cut(s) 26
BsiHKCI CYCGRG 2 cut(s) 318, 741
BsiSI CCGG 1 cut(s) 686
BslFI GGGAC 1 cut(s) 642
BslI CCNNNNNNNGG 2 cut(s) 537, 805
BsmAI GTCTC 2 cut(s) 473, 744
BsmFI GGGAC 1 cut(s) 642
BsnI GGCC 2 cut(s) 836, 955
BsoBI CYCGRG 2 cut(s) 318, 741
Bsp1286I GDGCHC 1 cut(s) 26
Bsp143I GATC 8 cut(s) 59, 145, 232, 428, 682, 790, 856, 865
BspACI CCGC 2 cut(s) 956, 971
BspANI GGCC 2 cut(s) 836, 955
BspCNI CTCAG 5 cut(s) 12, 266, 360, 376, 811
BspDI ATCGAT 1 cut(s) 859
BspFNI CGCG 4 cut(s) 40, 939, 958, 993
BspLI GGNNCC 3 cut(s) 684, 837, 984
BspMAI CTGCAG 1 cut(s) 495
BspMI ACCTGC 1 cut(s) 498
BspPI GGATC 6 cut(s) 67, 436, 677, 690, 785, 864
BsrBI CCGCTC 1 cut(s) 973
BsrDI GCAATG 1 cut(s) 354
BsrI ACTGG 2 cut(s) 325, 561
BssMI GATC 8 cut(s) 59, 145, 232, 428, 682, 790, 856, 865
Bst2UI CCWGG 2 cut(s) 311, 987
Bst6I CTCTTC 1 cut(s) 339
BstAPI GCANNNNNTGC 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 6 cut(s) 20, 253, 368, 384, 819, 999
BstEII GGTNACC 1 cut(s) 167
BstF5I GGATG 7 cut(s) 250, 547, 717, 868, 903, 907, 918
BstFNI CGCG 4 cut(s) 40, 939, 958, 993
BstHHI GCGC 1 cut(s) 261
BstKTI GATC 8 cut(s) 62, 148, 235, 431, 685, 793, 859, 868
BstMAI GTCTC 2 cut(s) 473, 744
BstMBI GATC 8 cut(s) 59, 145, 232, 428, 682, 790, 856, 865
BstMWI GCNNNNNNNGC 3 cut(s) 401, 581, 815
BstNI CCWGG 2 cut(s) 311, 987
BstPI GGTNACC 1 cut(s) 167
BstSCI CCNGG 2 cut(s) 309, 985
BstSFI CTRYAG 2 cut(s) 491, 658
BstUI CGCG 4 cut(s) 40, 939, 958, 993
BstV1I GCAGC 2 cut(s) 421, 917
BstV2I GAAGAC 1 cut(s) 426
BstX2I RGATCY 2 cut(s) 682, 790
BstXI CCANNNNNNTGG 2 cut(s) 592, 802
BstYI RGATCY 2 cut(s) 682, 790
Bsu15I ATCGAT 1 cut(s) 859
BsuRI GGCC 2 cut(s) 836, 955
BsuTUI ATCGAT 1 cut(s) 859
BtsCI GGATG 7 cut(s) 250, 547, 717, 868, 903, 907, 918
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 3 cut(s) 17, 146, 268
BveI ACCTGC 1 cut(s) 498
Cac8I GCNNGC 1 cut(s) 381
CfoI GCGC 1 cut(s) 261
Cfr13I GGNCC 2 cut(s) 307, 835
ClaI ATCGAT 1 cut(s) 859
Csp6I GTAC 1 cut(s) 946
CviAII CATG 7 cut(s) 72, 130, 340, 599, 708, 785, 962
CviQI GTAC 1 cut(s) 946
DdeI CTNAG 6 cut(s) 20, 253, 368, 384, 819, 999
DpnI GATC 8 cut(s) 61, 147, 234, 430, 684, 792, 858, 867
DpnII GATC 8 cut(s) 59, 145, 232, 428, 682, 790, 856, 865
DraI TTTAAA 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Eco47I GGWCC 1 cut(s) 307
Eco88I CYCGRG 2 cut(s) 318, 741
Eco91I GGTNACC 1 cut(s) 167
EcoO65I GGTNACC 1 cut(s) 167
EcoRII CCWGG 2 cut(s) 309, 985
EcoT22I ATGCAT 2 cut(s) 334, 523
FaeI CATG 7 cut(s) 75, 133, 343, 602, 711, 788, 965
FaqI GGGAC 1 cut(s) 642
FatI CATG 7 cut(s) 71, 129, 339, 598, 707, 784, 961
FbaI TGATCA 2 cut(s) 145, 865
Fnu4HI GCNGC 3 cut(s) 410, 906, 956
FokI GGATG 7 cut(s) 257, 554, 724, 855, 905, 910, 914
Fsp4HI GCNGC 3 cut(s) 410, 906, 956
FspBI CTAG 1 cut(s) 405
GlaI GCGC 1 cut(s) 260
GluI GCNGC 3 cut(s) 410, 906, 956
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 578
HaeIII GGCC 2 cut(s) 836, 955
HapII CCGG 1 cut(s) 686
HhaI GCGC 1 cut(s) 261
Hin1II CATG 7 cut(s) 75, 133, 343, 602, 711, 788, 965
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 588
HinfI GANTC 4 cut(s) 321, 566, 698, 826
HpaII CCGG 1 cut(s) 686
HphI GGTGA 2 cut(s) 242, 923
Hpy188I TCNGA 2 cut(s) 244, 256
Hpy188III TCNNGA 2 cut(s) 160, 452
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 3 cut(s) 78, 809, 892
HpyCH4IV ACGT 1 cut(s) 35
HpyF10VI GCNNNNNNNGC 3 cut(s) 401, 581, 815
HpyF3I CTNAG 6 cut(s) 20, 253, 368, 384, 819, 999
HpySE526I ACGT 1 cut(s) 35
Hsp92II CATG 7 cut(s) 75, 133, 343, 602, 711, 788, 965
HspAI GCGC 1 cut(s) 259
Ksp22I TGATCA 2 cut(s) 145, 865
Kzo9I GATC 8 cut(s) 59, 145, 232, 428, 682, 790, 856, 865
LmnI GCTCC 1 cut(s) 982
Lsp1109I GCAGC 2 cut(s) 421, 917
LweI GCATC 4 cut(s) 145, 235, 892, 985
MaeI CTAG 1 cut(s) 405
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 2 cut(s) 15, 167
MalI GATC 8 cut(s) 61, 147, 234, 430, 684, 792, 858, 867
MbiI CCGCTC 1 cut(s) 973
MboI GATC 8 cut(s) 59, 145, 232, 428, 682, 790, 856, 865
MboII GAAGA 4 cut(s) 286, 289, 356, 426
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 2 cut(s) 682, 790
MhlI GDGCHC 1 cut(s) 26
MluCI AATT 5 cut(s) 124, 501, 514, 546, 648
MlyI GAGTC 2 cut(s) 330, 575
MmeI TCCRAC 1 cut(s) 222
Mph1103I ATGCAT 2 cut(s) 334, 523
MseI TTAA 1 cut(s) 222
MslI CAYNNNNRTG 2 cut(s) 141, 603
MspA1I CMGCKG 2 cut(s) 304, 908
MspI CCGG 1 cut(s) 686
MspR9I CCNGG 2 cut(s) 311, 987
MunI CAATTG 1 cut(s) 501
MvaI CCWGG 2 cut(s) 311, 987
MvnI CGCG 4 cut(s) 40, 939, 958, 993
MwoI GCNNNNNNNGC 3 cut(s) 401, 581, 815
NdeII GATC 8 cut(s) 59, 145, 232, 428, 682, 790, 856, 865
NlaIII CATG 7 cut(s) 75, 133, 343, 602, 711, 788, 965
NlaIV GGNNCC 3 cut(s) 684, 837, 984
NmuCI GTSAC 1 cut(s) 15
NsiI ATGCAT 2 cut(s) 334, 523
PaeR7I CTCGAG 1 cut(s) 318
PfeI GAWTC 2 cut(s) 698, 826
PkrI GCNGC 3 cut(s) 411, 907, 957
PleI GAGTC 2 cut(s) 329, 574
PpsI GAGTC 2 cut(s) 329, 574
Psp6I CCWGG 2 cut(s) 309, 985
PspEI GGTNACC 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 2 cut(s) 309, 985
PspN4I GGNNCC 3 cut(s) 684, 837, 984
PspPI GGNCC 2 cut(s) 307, 835
PspXI VCTCGAGB 1 cut(s) 318
PstI CTGCAG 1 cut(s) 495
PsuI RGATCY 2 cut(s) 682, 790
PvuII CAGCTG 2 cut(s) 304, 908
RsaI GTAC 1 cut(s) 947
RsaNI GTAC 1 cut(s) 946
RseI CAYNNNNRTG 2 cut(s) 141, 603
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 3 cut(s) 410, 906, 956
Sau3AI GATC 8 cut(s) 59, 145, 232, 428, 682, 790, 856, 865
Sau96I GGNCC 2 cut(s) 307, 835
SbfI CCTGCAGG 1 cut(s) 495
SchI GAGTC 2 cut(s) 330, 575
ScrFI CCNGG 2 cut(s) 311, 987
SdaI CCTGCAGG 1 cut(s) 495
SduI GDGCHC 1 cut(s) 26
SfaNI GCATC 4 cut(s) 145, 235, 892, 985
SfcI CTRYAG 2 cut(s) 491, 658
Sfr274I CTCGAG 1 cut(s) 318
SinI GGWCC 1 cut(s) 307
SlaI CTCGAG 1 cut(s) 318
SmiMI CAYNNNNRTG 2 cut(s) 141, 603
SmlI CTYRAG 3 cut(s) 318, 450, 723
SmoI CTYRAG 3 cut(s) 318, 450, 723
Sse8387I CCTGCAGG 1 cut(s) 495
Sse9I AATT 5 cut(s) 124, 501, 514, 546, 648
SsiI CCGC 2 cut(s) 956, 971
SspMI CTAG 1 cut(s) 405
StyD4I CCNGG 2 cut(s) 309, 985
TaiI ACGT 1 cut(s) 38
TaqI TCGA 3 cut(s) 319, 859, 975
TasI AATT 5 cut(s) 124, 501, 514, 546, 648
TatI WGTACW 1 cut(s) 945
TauI GCSGC 1 cut(s) 958
TfiI GAWTC 2 cut(s) 698, 826
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 3 cut(s) 24, 146, 268
TseFI GTSAC 1 cut(s) 15
TseI GCWGC 2 cut(s) 409, 905
Tsp45I GTSAC 1 cut(s) 15
TspDTI ATGAA 9 cut(s) 60, 118, 558, 606, 615, 696, 773, 907, 978
TspRI CASTG 3 cut(s) 24, 146, 268
VpaK11BI GGWCC 1 cut(s) 307
XapI RAATTY 3 cut(s) 124, 546, 648
XcmI CCANNNNNNNNNTGG 1 cut(s) 802
XhoI CTCGAG 1 cut(s) 318
XspI CTAG 1 cut(s) 405
Zsp2I ATGCAT 2 cut(s) 334, 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.