pycom13g06530

Protein PHYTOCHROME KINASE SUBSTRATE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013994
Physical Location & Seq
Forward (+)
4238483 .. 4239982
1500 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1500 bp
ATGGCCATTGTTACCTCAAAATCAACTAGTAAGGCGAACCTCTCGCAAACTTTTCCCTTCGAGAACGCCGTCGCCATCCGTGATGCTTCATTTTCTTCATACTTTAGCGGCATTGAGGAAGCCGTCGTGCATAAACTCGCCGATCAGGCGAGCAAGGAGCAGCAGCATAACAATAACAATCACTTGGTAGCAAAAAAGGAAGCAGCTGACGATGAAGAAATTGGAGTGTTCAGAGCTGAAAAATACTTCAATGGAGTAATAGATGAGGAGACAAGCGTCACAAGCCCGAAAAGCGGCAGCAGCAGCACAAATAAGTACCATCATAACAAGGATAAACCAGTGAGTATGGATCACGTGAAGATAAGGGTTCAGCCGGGGACTCCGAGTGTGCGTTCTGAATCGAGCTGGAACAGCCAAAGCACCTTGCTTCGAAGCGCGCCGAGAAATCCTTCCCGGACAAACACAACTACGGGAAACAAACAGAGGAAGAACTTCTTTGCCAGCCTTGGCTGCAAATGTTCGTGTTCAGATAAGAATTCGGTTGATGTGGATGAACACGTCGGTGAGATCAGCTTCAAGAAGAACAATGGTGCAAACACAAGCTGCACGACAACACCAATTAATCATAAACCTCCTGGTGTGGCAGAGATTGTTGATGAAGTTCTAGTTGATATGAACAAAAGCGAAAAGCTCGAAAAGTTGGGAGTTGAGTTGGGAAGAGAGAACTGTTTTACTTTCCCAACTTTAAAATCCGGGGCGGGCAGTTTGCCGCCGAAGATGCTTCCCTTTCACCAAGCAGTAGAGGAAGCAGAGAAAGTGAGGAAGTCTCTGGAGGTGTTCGGCTCCCCTGTGTTTGAGAAGCAAAATAAGTCTTTCGCCCTTGGAAAAAAGCTCACAATGCTGCCGCGGGATGAGTTTCAGGCAAGTTCTGGTGGTGTAATCTACAACAATGACTCGGATAGTGATGCAAGTTCGGATCTTTTCGAGATTGAAAGCCTCACCGGCAAAGCAAACCCTTTTCTTGCGCGGCAAGCATCAGATGCTGGCTCTGCGACGCCAACGAATTGTTATGCGCCGAGTGAGGCGAGCATAGAGTGGAGTGTGGTCACCGCCAGCATTGCTGACATATCCATGATGTCCGACTTCGAAGATCAACAGAGACCAGCGAAAATGGCTCCCAATTCTACGAATGCCAATGCAAGAATGAGCAAGGAAATTCCGAGGCACCACTCCGGGGCACTGTTGGGTTGTAAGAGCCATAAATCTGTGAAAGTTGCAGGAGAGGCACACAGAAGTACATATCAACACGAGAAGAACAATTTCGAACCACAGATCACACGCCGCCGGGCAGAATCATACGTGGCAGGGACAAGGTTTCAAGCTGAGACAACTAATAAGCTGGCAGGCTTTGATGCCAGAGTACAAGGGCAACATGCTCTTGCAGCACGGCCGCTCCCTCGTTCACATTCACCACATGCTTCACATCTTTTGTTCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

500

Amino Acids

54.55

Weight (kDa)

8.32

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013994)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14280 AT2G02950
fragaria_vesca FvH4_4g31450
malus_domestica MD16G1073600.v1.1
prunus_persica Prupe.1G273300_v2.0.a1
pyrus_communis pycom13g06530
rosa_chinensis RchiOBHm_Chr4g0439931
rosa_laevigata RLG00000006224
rosa_multiflora Rmu_sc0006000.1_g000001 Rmu_sc0022459.1_g000001
rosa_roxburghii Rroxscaffold_5G00380880
rosa_rugosa Rorug04G0322600
rosa_samantha Rh4AG373500 Rh4BG385800 Rh4CG400300 Rh4DG380100
rosa_wichuraiana Rw4G031940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1224
AccBSI CCGCTC 1 cut(s) 1453
AccII CGCG 3 cut(s) 437, 907, 1027
AclWI GGATC 2 cut(s) 357, 984
AcoI YGGCCR 2 cut(s) 3, 1448
AcsI RAATTY 2 cut(s) 535, 1215
AcvI CACGTG 1 cut(s) 355
AcyI GRCGYC 1 cut(s) 1055
AfaI GTAC 3 cut(s) 317, 1297, 1422
AfiI CCNNNNNNNGG 2 cut(s) 293, 1234
AflIII ACRYGT 1 cut(s) 556
AgsI TTSAA 4 cut(s) 250, 577, 992, 1379
AhlI ACTAGT 1 cut(s) 26
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 1 cut(s) 634
AleI CACNNNNGTG 1 cut(s) 561
AluBI AGCT 9 cut(s) 206, 236, 405, 573, 603, 691, 892, 1382, 1399
AluI AGCT 9 cut(s) 206, 236, 405, 573, 603, 691, 892, 1382, 1399
Alw26I GTCTC 4 cut(s) 263, 831, 1153, 1379
AlwI GGATC 2 cut(s) 357, 984
AlwNI CAGNNNCTG 1 cut(s) 1043
AoxI GGCC 2 cut(s) 3, 1448
ApoI RAATTY 2 cut(s) 535, 1215
AseI ATTAAT 1 cut(s) 621
Asp700I GAANNNNTTC 3 cut(s) 448, 491, 1319
AspLEI GCGC 4 cut(s) 437, 439, 1027, 1075
AsuC2I CCSGG 5 cut(s) 375, 454, 754, 1234, 1346
AsuHPI GGTGA 5 cut(s) 575, 782, 991, 1099, 1461
AsuII TTCGAA 3 cut(s) 430, 1146, 1323
BaeGI GKGCMC 1 cut(s) 1240
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 1224
BauI CACGAG 1 cut(s) 1307
BbrPI CACGTG 1 cut(s) 355
BccI CCATC 2 cut(s) 83, 327
BceAI ACGGC 3 cut(s) 53, 107, 1463
BciT130I CCWGG 1 cut(s) 636
BcnI CCSGG 5 cut(s) 375, 454, 754, 1234, 1346
BcoDI GTCTC 4 cut(s) 263, 831, 1153, 1379
BcuI ACTAGT 1 cut(s) 26
BfaI CTAG 2 cut(s) 27, 665
BglI GCCNNNNNGGC 2 cut(s) 146, 1002
Bme1390I CCNGG 6 cut(s) 375, 454, 636, 754, 1234, 1346
BmgBI CACGTC 1 cut(s) 559
BmiI GGNNCC 3 cut(s) 844, 1176, 1226
BmrFI CCNGG 6 cut(s) 375, 454, 636, 754, 1234, 1346
BmsI GCATC 6 cut(s) 73, 768, 955, 1030, 1043, 1402
BoxI GACNNNNGTC 1 cut(s) 275
BplI GAGNNNNNCTC 2 cut(s) 811, 843
BpmI CTGGAG 1 cut(s) 851
Bpu14I TTCGAA 3 cut(s) 430, 1146, 1323
BpuMI CCSGG 5 cut(s) 375, 454, 754, 1234, 1346
BsaAI YACGTR 2 cut(s) 355, 1360
BsaHI GRCGYC 1 cut(s) 1055
BsaI GGTCTC 1 cut(s) 1153
BsaJI CCNNGG 7 cut(s) 374, 505, 753, 880, 905, 1220, 1233
BsaXI ACNNNNNCTCC 2 cut(s) 1437, 1467
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 1234
Bse118I RCCGGY 1 cut(s) 1001
Bse1I ACTGG 1 cut(s) 338
Bse3DI GCAATG 1 cut(s) 1116
BseBI CCWGG 1 cut(s) 636
BseDI CCNNGG 7 cut(s) 374, 505, 753, 880, 905, 1220, 1233
BseGI GGATG 3 cut(s) 75, 556, 916
BseLI CCNNNNNNNGG 2 cut(s) 293, 1234
BseMI GCAATG 1 cut(s) 1116
BseMII CTCAG 1 cut(s) 1374
BseNI ACTGG 1 cut(s) 338
BsePI GCGCGC 1 cut(s) 435
BseRI GAGGAG 1 cut(s) 281
BseSI GKGCMC 1 cut(s) 1240
BseX3I CGGCCG 1 cut(s) 1448
BsgI GTGCAG 1 cut(s) 589
Bsh1236I CGCG 3 cut(s) 437, 907, 1027
Bsh1285I CGRYCG 1 cut(s) 1451
BshFI GGCC 2 cut(s) 5, 1450
BshNI GGYRCC 1 cut(s) 1224
BsiEI CGRYCG 1 cut(s) 1451
BsiSI CCGG 6 cut(s) 374, 454, 753, 1002, 1233, 1345
BslFI GGGAC 2 cut(s) 391, 1381
BslI CCNNNNNNNGG 2 cut(s) 293, 1234
BsmAI GTCTC 4 cut(s) 263, 831, 1153, 1379
BsmFI GGGAC 2 cut(s) 391, 1381
BsmI GAATGC 1 cut(s) 1195
BsnI GGCC 2 cut(s) 5, 1450
Bso31I GGTCTC 1 cut(s) 1153
Bsp119I TTCGAA 3 cut(s) 430, 1146, 1323
Bsp1286I GDGCHC 1 cut(s) 1240
Bsp143I GATC 6 cut(s) 142, 349, 567, 976, 1150, 1332
BspANI GGCC 2 cut(s) 5, 1450
BspCNI CTCAG 1 cut(s) 1375
BspFNI CGCG 3 cut(s) 437, 907, 1027
BspLI GGNNCC 3 cut(s) 844, 1176, 1226
BspPI GGATC 2 cut(s) 357, 984
BspT104I TTCGAA 3 cut(s) 430, 1146, 1323
BspT107I GGYRCC 1 cut(s) 1224
BspTNI GGTCTC 1 cut(s) 1153
BsrBI CCGCTC 1 cut(s) 1453
BsrDI GCAATG 1 cut(s) 1116
BsrFI RCCGGY 1 cut(s) 1001
BsrI ACTGG 1 cut(s) 338
BssAI RCCGGY 1 cut(s) 1001
BssECI CCNNGG 7 cut(s) 374, 505, 753, 880, 905, 1220, 1233
BssHII GCGCGC 1 cut(s) 435
BssMI GATC 6 cut(s) 142, 349, 567, 976, 1150, 1332
BssNI GRCGYC 1 cut(s) 1055
BssSI CACGAG 1 cut(s) 1307
BssT1I CCWWGG 2 cut(s) 505, 880
Bst2BI CACGAG 1 cut(s) 1307
Bst2UI CCWGG 1 cut(s) 636
Bst4CI ACNGT 2 cut(s) 728, 1242
Bst6I CTCTTC 1 cut(s) 712
BstACI GRCGYC 1 cut(s) 1055
BstAPI GCANNNNNTGC 1 cut(s) 1040
BstBAI YACGTR 2 cut(s) 355, 1360
BstBI TTCGAA 3 cut(s) 430, 1146, 1323
BstDEI CTNAG 1 cut(s) 1383
BstDSI CCRYGG 1 cut(s) 905
BstEII GGTNACC 1 cut(s) 1105
BstF5I GGATG 3 cut(s) 75, 556, 916
BstFNI CGCG 3 cut(s) 437, 907, 1027
BstHHI GCGC 4 cut(s) 437, 439, 1027, 1075
BstKTI GATC 6 cut(s) 145, 352, 570, 979, 1153, 1335
BstMAI GTCTC 4 cut(s) 263, 831, 1153, 1379
BstMBI GATC 6 cut(s) 142, 349, 567, 976, 1150, 1332
BstMCI CGRYCG 1 cut(s) 1451
BstNI CCWGG 1 cut(s) 636
BstNSI RCATGY 2 cut(s) 1436, 1478
BstPAI GACNNNNGTC 1 cut(s) 275
BstPI GGTNACC 1 cut(s) 1105
BstSCI CCNGG 6 cut(s) 373, 452, 634, 752, 1232, 1344
BstSLI GKGCMC 1 cut(s) 1240
BstUI CGCG 3 cut(s) 437, 907, 1027
BstX2I RGATCY 1 cut(s) 976
BstYI RGATCY 1 cut(s) 976
BstZI CGGCCG 1 cut(s) 1448
BsuRI GGCC 2 cut(s) 5, 1450
BtgI CCRYGG 1 cut(s) 905
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 3 cut(s) 75, 556, 916
BtsIMutI CAGTG 2 cut(s) 345, 1238
CaiI CAGNNNCTG 1 cut(s) 1043
CfoI GCGC 4 cut(s) 437, 439, 1027, 1075
Cfr10I RCCGGY 1 cut(s) 1001
Cfr42I CCGCGG 1 cut(s) 908
CseI GACGC 2 cut(s) 265, 1063
Csp6I GTAC 3 cut(s) 316, 1296, 1421
CviAII CATG 4 cut(s) 1132, 1433, 1475, 1495
CviQI GTAC 3 cut(s) 316, 1296, 1421
DdeI CTNAG 1 cut(s) 1383
DpnI GATC 6 cut(s) 144, 351, 569, 978, 1152, 1334
DpnII GATC 6 cut(s) 142, 349, 567, 976, 1150, 1332
DraI TTTAAA 1 cut(s) 747
EaeI YGGCCR 2 cut(s) 3, 1448
EagI CGGCCG 1 cut(s) 1448
Eam1104I CTCTTC 1 cut(s) 712
EarI CTCTTC 1 cut(s) 712
EclXI CGGCCG 1 cut(s) 1448
Eco130I CCWWGG 2 cut(s) 505, 880
Eco31I GGTCTC 1 cut(s) 1153
Eco52I CGGCCG 1 cut(s) 1448
Eco72I CACGTG 1 cut(s) 355
Eco91I GGTNACC 1 cut(s) 1105
EcoO65I GGTNACC 1 cut(s) 1105
EcoRI GAATTC 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 634
EcoT14I CCWWGG 2 cut(s) 505, 880
ErhI CCWWGG 2 cut(s) 505, 880
FaeI CATG 4 cut(s) 1135, 1436, 1478, 1498
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FaqI GGGAC 2 cut(s) 391, 1381
FatI CATG 4 cut(s) 1131, 1432, 1474, 1494
FauI CCCGC 2 cut(s) 751, 900
FokI GGATG 3 cut(s) 62, 563, 923
FspBI CTAG 2 cut(s) 27, 665
GlaI GCGC 4 cut(s) 436, 438, 1026, 1074
GsuI CTGGAG 1 cut(s) 851
HaeIII GGCC 2 cut(s) 5, 1450
HapII CCGG 6 cut(s) 374, 454, 753, 1002, 1233, 1345
HgaI GACGC 2 cut(s) 265, 1063
HhaI GCGC 4 cut(s) 437, 439, 1027, 1075
Hin1I GRCGYC 1 cut(s) 1055
Hin1II CATG 4 cut(s) 1135, 1436, 1478, 1498
Hin6I GCGC 4 cut(s) 435, 437, 1025, 1073
HinP1I GCGC 4 cut(s) 435, 437, 1025, 1073
HinfI GANTC 4 cut(s) 379, 398, 953, 1352
HpaII CCGG 6 cut(s) 374, 454, 753, 1002, 1233, 1345
HphI GGTGA 5 cut(s) 575, 782, 991, 1099, 1461
Hpy166II GTNNAC 1 cut(s) 1463
Hpy188I TCNGA 9 cut(s) 233, 384, 397, 529, 958, 976, 1039, 1141, 1221
Hpy188III TCNNGA 4 cut(s) 61, 577, 830, 985
Hpy8I GTNNAC 1 cut(s) 1463
Hpy99I CGWCG 4 cut(s) 74, 128, 563, 1057
HpyAV CCTTC 2 cut(s) 67, 459
HpyCH4III ACNGT 2 cut(s) 728, 1242
HpyCH4IV ACGT 3 cut(s) 354, 558, 1359
HpyCH4V TGCA 8 cut(s) 130, 513, 593, 606, 968, 1199, 1277, 1442
HpyF3I CTNAG 1 cut(s) 1383
HpySE526I ACGT 3 cut(s) 354, 558, 1359
Hsp92I GRCGYC 1 cut(s) 1055
Hsp92II CATG 4 cut(s) 1135, 1436, 1478, 1498
HspAI GCGC 4 cut(s) 435, 437, 1025, 1073
KspI CCGCGG 1 cut(s) 908
Kzo9I GATC 6 cut(s) 142, 349, 567, 976, 1150, 1332
LmnI GCTCC 4 cut(s) 157, 848, 1180, 1458
LweI GCATC 6 cut(s) 73, 768, 955, 1030, 1043, 1402
MaeI CTAG 2 cut(s) 27, 665
MaeII ACGT 3 cut(s) 354, 558, 1359
MaeIII GTNAC 3 cut(s) 10, 277, 1105
MalI GATC 6 cut(s) 144, 351, 569, 978, 1152, 1334
MbiI CCGCTC 1 cut(s) 1453
MboI GATC 6 cut(s) 142, 349, 567, 976, 1150, 1332
MboII GAAGA 9 cut(s) 87, 227, 370, 499, 592, 729, 787, 1160, 1324
MflI RGATCY 1 cut(s) 976
MhlI GDGCHC 1 cut(s) 1240
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 219, 535, 618, 1063, 1180, 1215, 1318
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 373, 947
MmeI TCCRAC 1 cut(s) 1164
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 3 cut(s) 448, 491, 1319
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 621, 746
MslI CAYNNNNRTG 2 cut(s) 561, 1130
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 206, 907
MspI CCGG 6 cut(s) 374, 454, 753, 1002, 1233, 1345
MspR9I CCNGG 6 cut(s) 375, 454, 636, 754, 1234, 1346
Mva1269I GAATGC 1 cut(s) 1195
MvaI CCWGG 1 cut(s) 636
MvnI CGCG 3 cut(s) 437, 907, 1027
NciI CCSGG 5 cut(s) 375, 454, 754, 1234, 1346
NdeII GATC 6 cut(s) 142, 349, 567, 976, 1150, 1332
NlaIII CATG 4 cut(s) 1135, 1436, 1478, 1498
NlaIV GGNNCC 3 cut(s) 844, 1176, 1226
NmeAIII GCCGAG 2 cut(s) 465, 1101
NmuCI GTSAC 2 cut(s) 277, 1105
NspI RCATGY 2 cut(s) 1436, 1478
NspV TTCGAA 3 cut(s) 430, 1146, 1323
OliI CACNNNNGTG 1 cut(s) 561
PauI GCGCGC 1 cut(s) 435
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 1195
PdmI GAANNNNTTC 3 cut(s) 448, 491, 1319
PfeI GAWTC 2 cut(s) 398, 1352
PfoI TCCNGGA 1 cut(s) 452
PleI GAGTC 2 cut(s) 373, 947
PmaCI CACGTG 1 cut(s) 355
PmlI CACGTG 1 cut(s) 355
PpsI GAGTC 2 cut(s) 373, 947
Ppu21I YACGTR 2 cut(s) 355, 1360
PshAI GACNNNNGTC 1 cut(s) 275
PshBI ATTAAT 1 cut(s) 621
Psp6I CCWGG 1 cut(s) 634
PspCI CACGTG 1 cut(s) 355
PspEI GGTNACC 1 cut(s) 1105
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 3 cut(s) 844, 1176, 1226
PstNI CAGNNNCTG 1 cut(s) 1043
PsuI RGATCY 1 cut(s) 976
PteI GCGCGC 1 cut(s) 435
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 3 cut(s) 317, 1297, 1422
RsaNI GTAC 3 cut(s) 316, 1296, 1421
RseI CAYNNNNRTG 2 cut(s) 561, 1130
SacII CCGCGG 1 cut(s) 908
SaqAI TTAA 2 cut(s) 621, 746
Sau3AI GATC 6 cut(s) 142, 349, 567, 976, 1150, 1332
SchI GAGTC 2 cut(s) 373, 947
ScrFI CCNGG 6 cut(s) 375, 454, 636, 754, 1234, 1346
SduI GDGCHC 1 cut(s) 1240
SfaNI GCATC 6 cut(s) 73, 768, 955, 1030, 1043, 1402
Sfr303I CCGCGG 1 cut(s) 908
SfuI TTCGAA 3 cut(s) 430, 1146, 1323
SgrBI CCGCGG 1 cut(s) 908
SmiMI CAYNNNNRTG 2 cut(s) 561, 1130
SpeI ACTAGT 1 cut(s) 26
Sse9I AATT 7 cut(s) 219, 535, 618, 1063, 1180, 1215, 1318
SspMI CTAG 2 cut(s) 27, 665
StyD4I CCNGG 6 cut(s) 373, 452, 634, 752, 1232, 1344
StyI CCWWGG 2 cut(s) 505, 880
TaaI ACNGT 2 cut(s) 728, 1242
TaiI ACGT 3 cut(s) 357, 561, 1362
TaqI TCGA 7 cut(s) 60, 401, 430, 693, 984, 1146, 1323
TasI AATT 7 cut(s) 219, 535, 618, 1063, 1180, 1215, 1318
TatI WGTACW 2 cut(s) 1295, 1420
TauI GCSGC 7 cut(s) 111, 297, 772, 907, 1030, 1344, 1453
TfiI GAWTC 2 cut(s) 398, 1352
Tru1I TTAA 2 cut(s) 621, 746
Tru9I TTAA 2 cut(s) 621, 746
TscAI CASTG 2 cut(s) 345, 1245
TseFI GTSAC 2 cut(s) 277, 1105
Tsp45I GTSAC 2 cut(s) 277, 1105
TspDTI ATGAA 7 cut(s) 78, 87, 228, 567, 672, 689, 1483
TspGWI ACGGA 1 cut(s) 68
TspRI CASTG 2 cut(s) 345, 1245
VspI ATTAAT 1 cut(s) 621
XapI RAATTY 2 cut(s) 535, 1215
XceI RCATGY 2 cut(s) 1436, 1478
XmnI GAANNNNTTC 3 cut(s) 448, 491, 1319
XspI CTAG 2 cut(s) 27, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.