pycom13g07230

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0018933
Physical Location & Seq
Forward (+)
4783939 .. 4786052
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 666 bp
ATGCCGTCTCATTCGGATCTGGACCGTCAGATCGAGCATCTGATGGAGTGCAAGACGTTGCCGGAGGCGGAGGTGAAGACGCTGTGCGAGCAGTCGAGATCGATCCTGGTGGAGGAGTGGAACGTGCAGCCGGTGAAGTGCCCCGTCACGGTGTGCGGAGATATACACGGCCAGTTCTACGACCTCATTGAGCTTTTTAAGATAGGAGGGAACGCTCCCGATACTAATTACCTTTTTATGGGTGATTATGTAGATCGTGGGTACTATTCTGTGGAGACTGTCACGCTTCTGGTCGCCCTGAAAGTCCGTTATAGAGATAGAATTACAATCCTCAGAGGAAATCACGAGAGCCGGCAAATTACTCAAGTGTATGGTTTTTATGACGAATGCTTGAGAAAGTATGGGAATGCCAATGTCTGGAAGTTCTTTACCGATTTATTTGATTATCTTCCCCTGACAGCCCTCATTGAGAGTCAGGTCTTCTGTTTGCATGGGGGTCTTTCACCATCTTTGGACACATTGGACAATATCCGAGCTTTGGACCGTATACAGGAGGTTCCACATGAAGGACCAATGTGTGATCGATCTTGTGGTCTGATCCAGATGACCGCTGTGGGTGGGGAATATCTCCGCGGGGTGCTGGTTATACATTCGGGCAGGATATAG

Protein Analysis

222

Amino Acids

25.21

Weight (kDa)

5.23

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 48 - 190 2.7e-36 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 417
AccI GTMKAC 1 cut(s) 547
AccII CGCG 1 cut(s) 633
AciI CCGC 5 cut(s) 68, 156, 609, 631, 633
AclWI GGATC 3 cut(s) 24, 97, 592
AcoI YGGCCR 1 cut(s) 169
AdeI CACNNNGTG 1 cut(s) 153
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 7 cut(s) 112, 148, 238, 417, 538, 550, 566
AjnI CCWGG 1 cut(s) 105
AluBI AGCT 2 cut(s) 193, 536
AluI AGCT 2 cut(s) 193, 536
Alw26I GTCTC 2 cut(s) 12, 269
AlwI GGATC 3 cut(s) 24, 97, 592
AoxI GGCC 1 cut(s) 169
ApeKI GCWGC 1 cut(s) 127
AspS9I GGNCC 3 cut(s) 22, 541, 569
AsuHPI GGTGA 4 cut(s) 85, 145, 254, 495
AvaII GGWCC 3 cut(s) 22, 541, 569
BaeGI GKGCMC 1 cut(s) 143
BauI CACGAG 1 cut(s) 344
BbsI GAAGAC 2 cut(s) 83, 472
BbvI GCAGC 1 cut(s) 139
BccI CCATC 2 cut(s) 37, 514
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 1 cut(s) 107
BcoDI GTCTC 2 cut(s) 12, 269
BisI GCNGC 1 cut(s) 128
BlsI GCNGC 1 cut(s) 129
Bme1390I CCNGG 1 cut(s) 107
Bme18I GGWCC 3 cut(s) 22, 541, 569
BmgT120I GGNCC 3 cut(s) 22, 541, 569
BmiI GGNNCC 1 cut(s) 558
BmrFI CCNGG 1 cut(s) 107
BmsI GCATC 1 cut(s) 46
BpiI GAAGAC 2 cut(s) 83, 472
BpuEI CTTGAG 2 cut(s) 348, 412
Bsa29I ATCGAT 2 cut(s) 101, 583
BsaJI CCNNGG 1 cut(s) 631
Bsc4I CCNNNNNNNGG 7 cut(s) 112, 148, 238, 417, 538, 550, 566
Bse118I RCCGGY 2 cut(s) 130, 351
Bse1I ACTGG 1 cut(s) 172
BseBI CCWGG 1 cut(s) 107
BseCI ATCGAT 2 cut(s) 101, 583
BseDI CCNNGG 1 cut(s) 631
BseLI CCNNNNNNNGG 7 cut(s) 112, 148, 238, 417, 538, 550, 566
BseMII CTCAG 1 cut(s) 346
BseNI ACTGG 1 cut(s) 172
BseRI GAGGAG 1 cut(s) 128
BseSI GKGCMC 1 cut(s) 143
BseXI GCAGC 1 cut(s) 139
BsgI GTGCAG 1 cut(s) 146
Bsh1236I CGCG 1 cut(s) 633
BshFI GGCC 1 cut(s) 171
BshVI ATCGAT 2 cut(s) 101, 583
BsiSI CCGG 3 cut(s) 62, 131, 352
BslI CCNNNNNNNGG 7 cut(s) 112, 148, 238, 417, 538, 550, 566
BsmAI GTCTC 2 cut(s) 12, 269
BsmBI CGTCTC 1 cut(s) 12
BsmI GAATGC 2 cut(s) 392, 412
BsnI GGCC 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 143
Bsp143I GATC 8 cut(s) 16, 30, 98, 102, 253, 580, 584, 597
BspACI CCGC 5 cut(s) 68, 156, 609, 631, 633
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 1 cut(s) 345
BspDI ATCGAT 2 cut(s) 101, 583
BspFNI CGCG 1 cut(s) 633
BspLI GGNNCC 1 cut(s) 558
BspPI GGATC 3 cut(s) 24, 97, 592
BsrFI RCCGGY 2 cut(s) 130, 351
BsrI ACTGG 1 cut(s) 172
BssAI RCCGGY 2 cut(s) 130, 351
BssECI CCNNGG 1 cut(s) 631
BssMI GATC 8 cut(s) 16, 30, 98, 102, 253, 580, 584, 597
BssNAI GTATAC 1 cut(s) 548
BssSI CACGAG 1 cut(s) 344
Bst1107I GTATAC 1 cut(s) 548
Bst2BI CACGAG 1 cut(s) 344
Bst2UI CCWGG 1 cut(s) 107
Bst4CI ACNGT 4 cut(s) 26, 151, 280, 545
BstC8I GCNNGC 2 cut(s) 89, 353
BstDEI CTNAG 1 cut(s) 332
BstDSI CCRYGG 1 cut(s) 631
BstENI CCTNNNNNAGG 1 cut(s) 110
BstFNI CGCG 1 cut(s) 633
BstKTI GATC 8 cut(s) 19, 33, 101, 105, 256, 583, 587, 600
BstMAI GTCTC 2 cut(s) 12, 269
BstMBI GATC 8 cut(s) 16, 30, 98, 102, 253, 580, 584, 597
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstNI CCWGG 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 105
BstSLI GKGCMC 1 cut(s) 143
BstUI CGCG 1 cut(s) 633
BstV1I GCAGC 1 cut(s) 139
BstV2I GAAGAC 2 cut(s) 83, 472
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BstZ17I GTATAC 1 cut(s) 548
Bsu15I ATCGAT 2 cut(s) 101, 583
BsuRI GGCC 1 cut(s) 171
BsuTUI ATCGAT 2 cut(s) 101, 583
BtgI CCRYGG 1 cut(s) 631
Cac8I GCNNGC 2 cut(s) 89, 353
Cfr10I RCCGGY 2 cut(s) 130, 351
Cfr13I GGNCC 3 cut(s) 22, 541, 569
Cfr42I CCGCGG 1 cut(s) 634
ClaI ATCGAT 2 cut(s) 101, 583
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 262
CviAII CATG 2 cut(s) 491, 563
CviJI RGCY 6 cut(s) 130, 171, 193, 351, 461, 536
CviKI_1 RGCY 6 cut(s) 130, 171, 193, 351, 461, 536
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 1 cut(s) 332
DpnI GATC 8 cut(s) 18, 32, 100, 104, 255, 582, 586, 599
DpnII GATC 8 cut(s) 16, 30, 98, 102, 253, 580, 584, 597
DraIII CACNNNGTG 1 cut(s) 153
EaeI YGGCCR 1 cut(s) 169
EciI GGCGGA 1 cut(s) 83
Eco47I GGWCC 3 cut(s) 22, 541, 569
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 105
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 2 cut(s) 494, 566
FatI CATG 2 cut(s) 490, 562
FauI CCCGC 1 cut(s) 626
FblI GTMKAC 1 cut(s) 547
Fnu4HI GCNGC 1 cut(s) 128
Fsp4HI GCNGC 1 cut(s) 128
GluI GCNGC 1 cut(s) 128
HaeIII GGCC 1 cut(s) 171
HapII CCGG 3 cut(s) 62, 131, 352
HgaI GACGC 1 cut(s) 88
Hin1II CATG 2 cut(s) 494, 566
HinfI GANTC 1 cut(s) 472
HpaII CCGG 3 cut(s) 62, 131, 352
HphI GGTGA 4 cut(s) 85, 145, 254, 495
Hpy166II GTNNAC 1 cut(s) 548
Hpy188I TCNGA 6 cut(s) 16, 30, 42, 335, 533, 597
Hpy188III TCNNGA 6 cut(s) 20, 96, 218, 344, 418, 601
Hpy8I GTNNAC 1 cut(s) 548
HpyAV CCTTC 1 cut(s) 560
HpyCH4III ACNGT 4 cut(s) 26, 151, 280, 545
HpyCH4IV ACGT 2 cut(s) 56, 123
HpyCH4V TGCA 3 cut(s) 51, 127, 490
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpyF3I CTNAG 1 cut(s) 332
HpySE526I ACGT 2 cut(s) 56, 123
Hsp92II CATG 2 cut(s) 494, 566
KroI GCCGGC 1 cut(s) 351
KroNI GCCGGC 1 cut(s) 353
KspI CCGCGG 1 cut(s) 634
Kzo9I GATC 8 cut(s) 16, 30, 98, 102, 253, 580, 584, 597
LmnI GCTCC 1 cut(s) 220
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 1 cut(s) 46
MaeII ACGT 2 cut(s) 56, 123
MaeIII GTNAC 2 cut(s) 145, 280
MalI GATC 8 cut(s) 18, 32, 100, 104, 255, 582, 586, 599
MboI GATC 8 cut(s) 16, 30, 98, 102, 253, 580, 584, 597
MboII GAAGA 3 cut(s) 88, 440, 472
MflI RGATCY 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 3 cut(s) 226, 321, 357
MlyI GAGTC 1 cut(s) 481
MnlI CCTC 9 cut(s) 58, 64, 106, 194, 200, 329, 341, 473, 547
MroNI GCCGGC 1 cut(s) 351
MseI TTAA 1 cut(s) 198
MspA1I CMGCKG 2 cut(s) 611, 633
MspI CCGG 3 cut(s) 62, 131, 352
MspR9I CCNGG 1 cut(s) 107
Mva1269I GAATGC 2 cut(s) 392, 412
MvaI CCWGG 1 cut(s) 107
MvnI CGCG 1 cut(s) 633
MwoI GCNNNNNNNGC 1 cut(s) 88
NaeI GCCGGC 1 cut(s) 353
NdeII GATC 8 cut(s) 16, 30, 98, 102, 253, 580, 584, 597
NgoMIV GCCGGC 1 cut(s) 351
NlaIII CATG 2 cut(s) 494, 566
NlaIV GGNNCC 1 cut(s) 558
NmuCI GTSAC 2 cut(s) 145, 280
PctI GAATGC 2 cut(s) 392, 412
PdiI GCCGGC 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 417
PkrI GCNGC 1 cut(s) 129
PleI GAGTC 1 cut(s) 480
PpsI GAGTC 1 cut(s) 480
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspN4I GGNNCC 1 cut(s) 558
PspPI GGNCC 3 cut(s) 22, 541, 569
PsuI RGATCY 1 cut(s) 16
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
SacII CCGCGG 1 cut(s) 634
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 1 cut(s) 128
Sau3AI GATC 8 cut(s) 16, 30, 98, 102, 253, 580, 584, 597
Sau96I GGNCC 3 cut(s) 22, 541, 569
SchI GAGTC 1 cut(s) 481
ScrFI CCNGG 1 cut(s) 107
SduI GDGCHC 1 cut(s) 143
SetI ASST 9 cut(s) 59, 75, 126, 186, 195, 234, 480, 538, 558
SfaNI GCATC 1 cut(s) 46
Sfr303I CCGCGG 1 cut(s) 634
SgrBI CCGCGG 1 cut(s) 634
SinI GGWCC 3 cut(s) 22, 541, 569
SmlI CTYRAG 2 cut(s) 363, 391
SmoI CTYRAG 2 cut(s) 363, 391
Sse9I AATT 3 cut(s) 226, 321, 357
SsiI CCGC 5 cut(s) 68, 156, 609, 631, 633
StyD4I CCNGG 1 cut(s) 105
TaaI ACNGT 4 cut(s) 26, 151, 280, 545
TaiI ACGT 2 cut(s) 59, 126
TaqI TCGA 4 cut(s) 33, 95, 101, 583
TasI AATT 3 cut(s) 226, 321, 357
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TseFI GTSAC 2 cut(s) 145, 280
TseI GCWGC 1 cut(s) 127
Tsp45I GTSAC 2 cut(s) 145, 280
TspDTI ATGAA 1 cut(s) 579
TspGWI ACGGA 1 cut(s) 296
Van91I CCANNNNNTGG 1 cut(s) 417
VpaK11BI GGWCC 3 cut(s) 22, 541, 569
XagI CCTNNNNNAGG 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.