pycom13g10120
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010877
Physical Location & Seq
Forward (+)
6780043 .. 6784184
4142 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2460 bp
ATGTATCGACGTAACATTCAACGTGTAAAGATAAATTCACATATTGAATTGAACATTGATAATTTATCTAAAAATTATATAGATGCCACCACTCCCTTCTCCTTCCTTTGCTTCTCTTCTACCCCTTGCTTCTTCCTCTCTCTTTTTGGCGGGAAGTGGACCCCGCCAATCAAATTTCCTCCTTCCGCTGGTCTCTTTCTCATAAATACCCGCAGCACCACCCCACCCACAGCTCCAACCACCCTCTCTCTCTCTAAACATGTACTTCAATTAAAACCCGCAATCTCCATCTCCTCCCATTTCTCCGGCCAAATTATGGAACCTTCCAGTTTCTCCGCCCTCCTCATACTCCTCCTCCTCGTCGACACCACACTCTCACTCAACTCCGACGGCCTCTCCCTCCTCTCTCTAAAATCCGCCGTTGACCAGCCCGCCGACGGCTCCGCCTTCTCCGACTGGCTCGACACCGACCCCACCCCATGCCGCTGGACCGGCATTTCCTGCATGAACATCTCCGGCTTCGCTGACCCCTGCGTCGTCGGAATCGCCATATCCGGGAAGAATCTCCGCGGCTACATTCCCTCCGAGCTCGGCAACCTAGTAAACCTCCGCCGCCTCAACCTCCACGACAACAACTTCCACGGCTCAATCCCTTCCCAACTCTTCAACGCTACCTTACTCCACAGCATCTTCCTCTACGGCAATAACCTCTCCGGCCCGCTTCCACCGTCGATCTGCAACCCCCCGCGTCTCCAGAACCTCGACCTGTCCAACAACTCCCTCTCCGGTCCGCTCCAGAAAGACTACTTGAAGAACTGCAAGCAATTGCAGCGGCTGATCCTCGCGAGAAACAGATTCGACGGAGAAATTCCGGCGGGAATTTGGTCCGGCATGAAGAATCTAAACCAATTGGATCTCTCCTCAAACGAGTTAACCGGATCGGTGCCGGACGATTTCAGCGAGTTGAAGTCTCTGTCGGGCGCGCTCAACCTATCGTACAATCACTTATCGGGAAAGATTCCGAAATCTCTCGGCGATTTACCCTTGACGGTGAGCTTGGATCTCCGAAACAACAATTTCAGCGGCGAGATTCCTCAAACGGGGTCGTTTTCGAACCAAGGCCCCACCGCGTTTCTGGACAACCCTCTGTTATGCGGGTTTCCGCTTCAGAAAACTTGTAAAAATCCCTCCGCGGATAGTCCGAATTCGGGATCAGGATCGGAAAATAGTCCGGTAAAGGGTATGAGTCCGGGTCTGATTATCCTAATCTCGGTGGCGGACGCCGCCGGAGTGGCGTTTATCGGATTAGTCGTCGTTTACATTTACTGGAAAAAGAAAGACGACGAGAACGGATGCAGTTGTACGGGAAAGAGCAAATTTGGCGGCAACGAGAAGCGCCACCTCTGTAATCTCTGTCATCTCTGTTCTTGTGCTTGCATCAATGGCGGGTTCGGGAACCAGGATTCGGATTCGGGCGACCCGGAAAAGGCAGAGAGGGGAAAAGGGGAGGGGGGTCTCGTCGCGATCGACAAAGGGTTCACATTCGAGCTGGACGAGCTGTTGAGGGCGTCGGCGTACGTGCTGGGGAAGAGCGGACTGGGGATCGTGTACAAAGTGGTGCTCGGGAATGGAATCCCGGTGGCGGTGAGGAGGTTGGGGGAGGGCGGGGATCAGAGGTATAAGGAGTTCGCGGCGGAGATTCAGGCGATCGGGCGGGTGAAGCATCCGAATGTGGTGAAGCTGAGGGCGTACTATTGGGCGCCCGACGAGAAGCTTCTCATCAGTGACTTCATTTCTAATGGCAACTTGGCCACTGCACTTCGCGGGAGAATCGGTCAGTCATCATCAAGCCTCTCATGGACAACGAGGCTAAAGATCGCTCATGGAACAGCCCGAGGTCTCGCCTACCTGCACGAATGCAGCCCCAGAAAGTTTGTCCACGGTGACATCAAACCCTCCAACATTCTCATCGACAACGAATATCAAGCCTACATTTCCGATTTCGGACTCAACAGGCTGATCACAATCACAGGCAACAACCCCTCCTCGGGTGGCTTCATGGGCGGGGCTCTCCCTTACATGCAACCAGTCCAAACAGAGCGATCAAATAGCTACCGTGCCCCGGAGGCCCGTGTCACGGGCAGCAAACCCACCCAAAAATGGGACGTGTACTCGTTTGGTGTTGTGTTGCTTGAATTGCTCACAGGGAAGTCCCCGGAGCTCTCTCCGACCACGTCAACTTCGGCCGAAGTCCCAGACCTGGTGAGCTCTGTGAGGAAAGGGTTTGAGGATGAAAACCCTTTGTCGGATATGGTAGACCATGTGTTGCTGCAAGAAGTGCATGCCAAAAAGGAGGTGCTTGCAGCTTTCCATGTAGCTCTTGCATGCACTGAGCCGGACCCTGAGGTTAGGCCAAGGATGAAAACCGTCTCTGAAAATCTTGAAAGGGTTGGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

820

Amino Acids

88.65

Weight (kDa)

7.78

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 127 - 168 3.9e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 275 - 325 6.8e-08 Leucine rich repeat
Pkinase PF00069 527 - 755 8.9e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 528 - 814 3.9e-30 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010877)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67510
fragaria_vesca FvH4_4g24010 FvH4_4g24010 FvH4_4g24010
malus_domestica MD13G1116600.v1.1 MD16G1117000.v1.1
prunus_persica Prupe.1G229200_v2.0.a1
pyrus_communis pycom13g10120 pycom16g10010
rosa_chinensis RchiOBHm_Chr4g0430671
rosa_laevigata RLG00000006957
rosa_multiflora Rmu_sc0000131.1_g000012 Rmu_sc0023719.1_g000001
rosa_roxburghii Rroxscaffold_5G00372410
rosa_rugosa Rorug04G0245100
rosa_samantha Rh4AG301200 Rh4BG308300 Rh4CG325500 Rh4DG305900
rosa_wichuraiana Rw4G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 533
Acc36I ACCTGC 1 cut(s) 1917
AccB1I GGYRCC 2 cut(s) 943, 1759
AccB7I CCANNNNNTGG 1 cut(s) 316
AccBSI CCGCTC 2 cut(s) 793, 1593
AccI GTMKAC 2 cut(s) 363, 2316
AccII CGCG 9 cut(s) 570, 748, 845, 983, 1130, 1193, 1523, 1691, 1824
AclWI GGATC 8 cut(s) 832, 921, 946, 1068, 1219, 1225, 1610, 1677
AcoI YGGCCR 3 cut(s) 307, 1809, 2244
AcsI RAATTY 6 cut(s) 34, 173, 867, 879, 1204, 1376
AcuI CTGAAG 1 cut(s) 1151
AcyI GRCGYC 3 cut(s) 1281, 1568, 1760
AfaI GTAC 7 cut(s) 264, 998, 1363, 1577, 1610, 1751, 2171
AflIII ACRYGT 3 cut(s) 22, 259, 2166
AgsI TTSAA 9 cut(s) 20, 47, 52, 269, 667, 811, 967, 2195, 2444
AjiI CACGTC 2 cut(s) 2167, 2235
AjnI CCWGG 2 cut(s) 1458, 2259
Alw21I GWGCWC 4 cut(s) 591, 1623, 2223, 2270
Alw26I GTCTC 6 cut(s) 197, 755, 975, 1520, 1904, 2434
AlwI GGATC 8 cut(s) 832, 921, 946, 1068, 1219, 1225, 1610, 1677
AlwNI CAGNNNCTG 1 cut(s) 835
Ama87I CYCGRG 3 cut(s) 1622, 1893, 2047
AoxI GGCC 8 cut(s) 307, 391, 715, 1120, 1809, 2127, 2244, 2411
ApeKI GCWGC 6 cut(s) 213, 829, 1920, 2142, 2329, 2363
ApoI RAATTY 6 cut(s) 34, 173, 867, 879, 1204, 1376
AspLEI GCGC 4 cut(s) 983, 985, 1398, 1762
AspS9I GGNCC 8 cut(s) 159, 489, 716, 788, 885, 1121, 2128, 2398
AsuC2I CCSGG 6 cut(s) 556, 1251, 1481, 1637, 2123, 2216
AsuHPI GGTGA 6 cut(s) 1063, 1657, 1729, 1747, 1955, 2275
AsuII TTCGAA 1 cut(s) 1112
AvaI CYCGRG 3 cut(s) 1622, 1893, 2047
AvaII GGWCC 5 cut(s) 159, 489, 788, 885, 2398
AxyI CCTNAGG 1 cut(s) 2403
BaeGI GKGCMC 1 cut(s) 2122
BalI TGGCCA 1 cut(s) 1811
BanI GGYRCC 2 cut(s) 943, 1759
BanII GRGCYC 4 cut(s) 591, 2071, 2223, 2270
Bbv12I GWGCWC 4 cut(s) 591, 1623, 2223, 2270
BbvCI CCTCAGC 1 cut(s) 1742
BbvI GCAGC 6 cut(s) 225, 841, 1932, 2154, 2316, 2375
BccI CCATC 1 cut(s) 296
BceAI ACGGC 5 cut(s) 404, 406, 454, 658, 715
BciT130I CCWGG 2 cut(s) 1460, 2261
BclI TGATCA 1 cut(s) 2019
BcnI CCSGG 6 cut(s) 556, 1251, 1481, 1637, 2123, 2216
BcoDI GTCTC 6 cut(s) 197, 755, 975, 1520, 1904, 2434
BfaI CTAG 1 cut(s) 599
BfoI RGCGCY 2 cut(s) 1399, 1763
BfuAI ACCTGC 1 cut(s) 1917
BglI GCCNNNNNGGC 2 cut(s) 1292, 2126
Bme1390I CCNGG 8 cut(s) 556, 1251, 1460, 1481, 1637, 2123, 2216, 2261
Bme18I GGWCC 5 cut(s) 159, 489, 788, 885, 2398
BmeT110I CYCGRG 3 cut(s) 1622, 1893, 2047
BmgBI CACGTC 2 cut(s) 2167, 2235
BmgT120I GGNCC 8 cut(s) 159, 489, 716, 788, 885, 1121, 2128, 2398
BmiI GGNNCC 8 cut(s) 161, 321, 442, 945, 1123, 1457, 1761, 2400
BmrFI CCNGG 8 cut(s) 556, 1251, 1460, 1481, 1637, 2123, 2216, 2261
BmrI ACTGGG 1 cut(s) 1607
BmsI GCATC 5 cut(s) 73, 696, 1343, 1446, 1732
BmuI ACTGGG 1 cut(s) 1607
BpmI CTGGAG 2 cut(s) 737, 779
Bpu10I CCTNAGC 1 cut(s) 1742
Bpu14I TTCGAA 1 cut(s) 1112
BpuMI CCSGG 6 cut(s) 556, 1251, 1481, 1637, 2123, 2216
BsaAI YACGTR 1 cut(s) 1579
BsaBI GATNNNNATC 2 cut(s) 1216, 2024
BsaHI GRCGYC 3 cut(s) 1281, 1568, 1760
BsaI GGTCTC 3 cut(s) 197, 1520, 1904
BsaWI WCCGGW 3 cut(s) 785, 935, 1231
Bse118I RCCGGY 1 cut(s) 491
Bse1I ACTGG 5 cut(s) 327, 461, 1331, 1602, 2087
Bse21I CCTNAGG 1 cut(s) 2403
Bse8I GATNNNNATC 2 cut(s) 1216, 2024
BseBI CCWGG 2 cut(s) 1460, 2261
BseGI GGATG 4 cut(s) 1358, 1723, 2296, 2424
BseJI GATNNNNATC 2 cut(s) 1216, 2024
BseMII CTCAG 3 cut(s) 1733, 2382, 2394
BseNI ACTGG 5 cut(s) 327, 461, 1331, 1602, 2087
BsePI GCGCGC 1 cut(s) 981
BseRI GAGGAG 9 cut(s) 283, 332, 341, 344, 347, 392, 910, 1663, 2035
BseSI GKGCMC 1 cut(s) 2122
BseX3I CGGCCG 1 cut(s) 2244
BseXI GCAGC 6 cut(s) 225, 841, 1932, 2154, 2316, 2375
BseYI CCCAGC 1 cut(s) 1582
BsgI GTGCAG 2 cut(s) 1800, 1895
Bsh1236I CGCG 9 cut(s) 570, 748, 845, 983, 1130, 1193, 1523, 1691, 1824
Bsh1285I CGRYCG 3 cut(s) 1527, 1710, 2247
BshFI GGCC 8 cut(s) 309, 393, 717, 1122, 1811, 2129, 2246, 2413
BshNI GGYRCC 2 cut(s) 943, 1759
BsiEI CGRYCG 3 cut(s) 1527, 1710, 2247
BsiHKAI GWGCWC 4 cut(s) 591, 1623, 2223, 2270
BsiHKCI CYCGRG 3 cut(s) 1622, 1893, 2047
BsiWI CGTACG 1 cut(s) 1575
BslFI GGGAC 3 cut(s) 2177, 2197, 2237
BsmAI GTCTC 6 cut(s) 197, 755, 975, 1520, 1904, 2434
BsmBI CGTCTC 2 cut(s) 755, 2434
BsmFI GGGAC 3 cut(s) 2177, 2197, 2237
BsmI GAATGC 1 cut(s) 1922
BsnI GGCC 8 cut(s) 309, 393, 717, 1122, 1811, 2129, 2246, 2413
Bso31I GGTCTC 3 cut(s) 197, 1520, 1904
BsoBI CYCGRG 3 cut(s) 1622, 1893, 2047
Bsp119I TTCGAA 1 cut(s) 1112
Bsp1286I GDGCHC 6 cut(s) 591, 1623, 2071, 2122, 2223, 2270
Bsp1407I TGTACA 1 cut(s) 1608
Bsp68I TCGCGA 2 cut(s) 845, 1523
BspANI GGCC 8 cut(s) 309, 393, 717, 1122, 1811, 2129, 2246, 2413
BspCNI CTCAG 3 cut(s) 1734, 2383, 2395
BspFNI CGCG 9 cut(s) 570, 748, 845, 983, 1130, 1193, 1523, 1691, 1824
BspLI GGNNCC 8 cut(s) 161, 321, 442, 945, 1123, 1457, 1761, 2400
BspMI ACCTGC 1 cut(s) 1917
BspPI GGATC 8 cut(s) 832, 921, 946, 1068, 1219, 1225, 1610, 1677
BspQI GCTCTTC 1 cut(s) 1583
BspT104I TTCGAA 1 cut(s) 1112
BspT107I GGYRCC 2 cut(s) 943, 1759
BspTNI GGTCTC 3 cut(s) 197, 1520, 1904
BsrBI CCGCTC 2 cut(s) 793, 1593
BsrFI RCCGGY 1 cut(s) 491
BsrGI TGTACA 1 cut(s) 1608
BsrI ACTGG 5 cut(s) 327, 461, 1331, 1602, 2087
BssAI RCCGGY 1 cut(s) 491
BssHII GCGCGC 1 cut(s) 981
BssNI GRCGYC 3 cut(s) 1281, 1568, 1760
BssT1I CCWWGG 2 cut(s) 1117, 2414
Bst2UI CCWGG 2 cut(s) 1460, 2261
Bst4CI ACNGT 5 cut(s) 729, 1051, 1943, 2117, 2428
Bst6I CTCTTC 3 cut(s) 121, 668, 1583
BstACI GRCGYC 3 cut(s) 1281, 1568, 1760
BstAPI GCANNNNNTGC 2 cut(s) 501, 2338
BstAUI TGTACA 1 cut(s) 1608
BstBAI YACGTR 1 cut(s) 1579
BstBI TTCGAA 1 cut(s) 1112
BstC8I GCNNGC 8 cut(s) 432, 719, 821, 983, 1435, 2343, 2361, 2386
BstDEI CTNAG 3 cut(s) 1742, 2391, 2403
BstDSI CCRYGG 4 cut(s) 568, 640, 1191, 1939
BstF5I GGATG 4 cut(s) 1358, 1723, 2296, 2424
BstFNI CGCG 9 cut(s) 570, 748, 845, 983, 1130, 1193, 1523, 1691, 1824
BstH2I RGCGCY 2 cut(s) 1399, 1763
BstHHI GCGC 4 cut(s) 983, 985, 1398, 1762
BstMAI GTCTC 6 cut(s) 197, 755, 975, 1520, 1904, 2434
BstMCI CGRYCG 3 cut(s) 1527, 1710, 2247
BstNI CCWGG 2 cut(s) 1460, 2261
BstNSI RCATGY 4 cut(s) 263, 2083, 2345, 2388
BstSCI CCNGG 8 cut(s) 554, 1249, 1458, 1479, 1635, 2121, 2214, 2259
BstSLI GKGCMC 1 cut(s) 2122
BstUI CGCG 9 cut(s) 570, 748, 845, 983, 1130, 1193, 1523, 1691, 1824
BstV1I GCAGC 6 cut(s) 225, 841, 1932, 2154, 2316, 2375
BstX2I RGATCY 2 cut(s) 913, 1060
BstXI CCANNNNNNTGG 1 cut(s) 486
BstYI RGATCY 2 cut(s) 913, 1060
BstZI CGGCCG 1 cut(s) 2244
Bsu36I CCTNAGG 1 cut(s) 2403
BsuRI GGCC 8 cut(s) 309, 393, 717, 1122, 1811, 2129, 2246, 2413
BtgI CCRYGG 4 cut(s) 568, 640, 1191, 1939
BtrI CACGTC 2 cut(s) 2167, 2235
BtsCI GGATG 4 cut(s) 1358, 1723, 2296, 2424
BtsI GCAGTG 1 cut(s) 1812
BtsIMutI CAGTG 3 cut(s) 1789, 1812, 2388
BtuMI TCGCGA 2 cut(s) 845, 1523
BveI ACCTGC 1 cut(s) 1917
Cac8I GCNNGC 8 cut(s) 432, 719, 821, 983, 1435, 2343, 2361, 2386
CaiI CAGNNNCTG 1 cut(s) 835
CfoI GCGC 4 cut(s) 983, 985, 1398, 1762
Cfr10I RCCGGY 1 cut(s) 491
Cfr13I GGNCC 8 cut(s) 159, 489, 716, 788, 885, 1121, 2128, 2398
Cfr42I CCGCGG 2 cut(s) 571, 1194
CpoI CGGWCCG 1 cut(s) 788
CseI GACGC 4 cut(s) 523, 737, 1289, 1557
CsiI ACCWGGT 1 cut(s) 2259
Csp6I GTAC 7 cut(s) 263, 997, 1362, 1576, 1609, 1750, 2170
CspI CGGWCCG 1 cut(s) 788
CviQI GTAC 7 cut(s) 263, 997, 1362, 1576, 1609, 1750, 2170
DdeI CTNAG 3 cut(s) 1742, 2391, 2403
DinI GGCGCC 1 cut(s) 1761
DrdI GACNNNNNNGTC 1 cut(s) 533
DseDI GACNNNNNNGTC 1 cut(s) 533
EaeI YGGCCR 3 cut(s) 307, 1809, 2244
EagI CGGCCG 1 cut(s) 2244
Eam1104I CTCTTC 3 cut(s) 121, 668, 1583
EarI CTCTTC 3 cut(s) 121, 668, 1583
EciI GGCGGA 6 cut(s) 325, 406, 433, 599, 1292, 1709
Ecl136II GAGCTC 3 cut(s) 589, 2221, 2268
EclXI CGGCCG 1 cut(s) 2244
Eco130I CCWWGG 2 cut(s) 1117, 2414
Eco24I GRGCYC 4 cut(s) 591, 2071, 2223, 2270
Eco31I GGTCTC 3 cut(s) 197, 1520, 1904
Eco47I GGWCC 5 cut(s) 159, 489, 788, 885, 2398
Eco52I CGGCCG 1 cut(s) 2244
Eco53kI GAGCTC 3 cut(s) 589, 2221, 2268
Eco57I CTGAAG 1 cut(s) 1151
Eco81I CCTNAGG 1 cut(s) 2403
Eco88I CYCGRG 3 cut(s) 1622, 1893, 2047
EcoICRI GAGCTC 3 cut(s) 589, 2221, 2268
EcoO109I RGGNCCY 1 cut(s) 1121
EcoRI GAATTC 1 cut(s) 1204
EcoRII CCWGG 2 cut(s) 1458, 2259
EcoT14I CCWWGG 2 cut(s) 1117, 2414
EcoT38I GRGCYC 4 cut(s) 591, 2071, 2223, 2270
EgeI GGCGCC 1 cut(s) 1761
EheI GGCGCC 1 cut(s) 1761
ErhI CCWWGG 2 cut(s) 1117, 2414
Esp3I CGTCTC 2 cut(s) 755, 2434
FaqI GGGAC 3 cut(s) 2177, 2197, 2237
FbaI TGATCA 1 cut(s) 2019
FblI GTMKAC 2 cut(s) 363, 2316
FokI GGATG 4 cut(s) 1365, 1710, 2303, 2431
FriOI GRGCYC 4 cut(s) 591, 2071, 2223, 2270
FspBI CTAG 1 cut(s) 599
GlaI GCGC 4 cut(s) 982, 984, 1397, 1761
GsaI CCCAGC 1 cut(s) 1586
GsuI CTGGAG 2 cut(s) 737, 779
HaeII RGCGCY 2 cut(s) 1399, 1763
HaeIII GGCC 8 cut(s) 309, 393, 717, 1122, 1811, 2129, 2246, 2413
HgaI GACGC 4 cut(s) 523, 737, 1289, 1557
HhaI GCGC 4 cut(s) 983, 985, 1398, 1762
Hin1I GRCGYC 3 cut(s) 1281, 1568, 1760
Hin6I GCGC 4 cut(s) 981, 983, 1396, 1760
HinP1I GCGC 4 cut(s) 981, 983, 1396, 1760
HincII GTYRAC 4 cut(s) 364, 424, 933, 2238
HindII GTYRAC 4 cut(s) 364, 424, 933, 2238
HindIII AAGCTT 1 cut(s) 1772
HpaI GTTAAC 1 cut(s) 933
HphI GGTGA 6 cut(s) 1063, 1657, 1729, 1747, 1955, 2275
HpyAV CCTTC 6 cut(s) 106, 112, 192, 333, 457, 663
HpyCH4III ACNGT 5 cut(s) 729, 1051, 1943, 2117, 2428
HpyCH4IV ACGT 5 cut(s) 10, 22, 1578, 2166, 2234
HpyF3I CTNAG 3 cut(s) 1742, 2391, 2403
HpySE526I ACGT 5 cut(s) 10, 22, 1578, 2166, 2234
Hsp92I GRCGYC 3 cut(s) 1281, 1568, 1760
HspAI GCGC 4 cut(s) 981, 983, 1396, 1760
KasI GGCGCC 1 cut(s) 1759
Ksp22I TGATCA 1 cut(s) 2019
KspAI GTTAAC 1 cut(s) 933
KspI CCGCGG 2 cut(s) 571, 1194
LguI GCTCTTC 1 cut(s) 1583
LmnI GCTCC 4 cut(s) 238, 446, 798, 2218
Lsp1109I GCAGC 6 cut(s) 225, 841, 1932, 2154, 2316, 2375
LweI GCATC 5 cut(s) 73, 696, 1343, 1446, 1732
MabI ACCWGGT 1 cut(s) 2259
MaeI CTAG 1 cut(s) 599
MaeII ACGT 5 cut(s) 10, 22, 1578, 2166, 2234
MaeIII GTNAC 4 cut(s) 11, 1784, 1943, 2134
MbiI CCGCTC 2 cut(s) 793, 1593
MboII GAAGA 8 cut(s) 108, 124, 571, 655, 682, 823, 907, 1600
MfeI CAATTG 2 cut(s) 824, 908
MflI RGATCY 2 cut(s) 913, 1060
MhlI GDGCHC 6 cut(s) 591, 1623, 2071, 2122, 2223, 2270
MlsI TGGCCA 1 cut(s) 1811
MluNI TGGCCA 1 cut(s) 1811
Mly113I GGCGCC 1 cut(s) 1760
MlyI GAGTC 2 cut(s) 1255, 2001
MmeI TCCRAC 9 cut(s) 260, 411, 477, 520, 795, 1983, 2252, 2286, 2431
Mox20I TGGCCA 1 cut(s) 1811
MscI TGGCCA 1 cut(s) 1811
MseI TTAA 2 cut(s) 272, 932
MslI CAYNNNNRTG 1 cut(s) 1728
Msp20I TGGCCA 1 cut(s) 1811
MspA1I CMGCKG 6 cut(s) 188, 486, 570, 832, 1083, 1193
MspR9I CCNGG 8 cut(s) 556, 1251, 1460, 1481, 1637, 2123, 2216, 2261
MunI CAATTG 2 cut(s) 824, 908
Mva1269I GAATGC 1 cut(s) 1922
MvaI CCWGG 2 cut(s) 1460, 2261
MvnI CGCG 9 cut(s) 570, 748, 845, 983, 1130, 1193, 1523, 1691, 1824
NarI GGCGCC 1 cut(s) 1760
NciI CCSGG 6 cut(s) 556, 1251, 1481, 1637, 2123, 2216
NlaIV GGNNCC 8 cut(s) 161, 321, 442, 945, 1123, 1457, 1761, 2400
NmeAIII GCCGAG 2 cut(s) 570, 1011
NmuCI GTSAC 3 cut(s) 1784, 1943, 2134
NruI TCGCGA 2 cut(s) 845, 1523
NspI RCATGY 4 cut(s) 263, 2083, 2345, 2388
NspV TTCGAA 1 cut(s) 1112
PaeI GCATGC 2 cut(s) 2345, 2388
PauI GCGCGC 1 cut(s) 981
PciI ACATGT 1 cut(s) 259
PciSI GCTCTTC 1 cut(s) 1583
PcsI WCGNNNNNNNCGW 4 cut(s) 957, 1308, 1524, 1551
PctI GAATGC 1 cut(s) 1922
Pfl23II CGTACG 1 cut(s) 1575
PflFI GACNNNGTC 1 cut(s) 2233
PflMI CCANNNNNTGG 1 cut(s) 316
PfoI TCCNGGA 1 cut(s) 554
Ple19I CGATCG 2 cut(s) 1527, 1710
PleI GAGTC 2 cut(s) 1254, 2001
PluTI GGCGCC 1 cut(s) 1763
PpsI GAGTC 2 cut(s) 1254, 2001
Ppu21I YACGTR 1 cut(s) 1579
PscI ACATGT 1 cut(s) 259
Psp124BI GAGCTC 3 cut(s) 591, 2223, 2270
Psp6I CCWGG 2 cut(s) 1458, 2259
PspFI CCCAGC 1 cut(s) 1582
PspGI CCWGG 2 cut(s) 1458, 2259
PspLI CGTACG 1 cut(s) 1575
PspN4I GGNNCC 8 cut(s) 161, 321, 442, 945, 1123, 1457, 1761, 2400
PspPI GGNCC 8 cut(s) 159, 489, 716, 788, 885, 1121, 2128, 2398
PsrI GAACNNNNNNTAC 2 cut(s) 1670, 1702
PstNI CAGNNNCTG 1 cut(s) 835
PsuI RGATCY 2 cut(s) 913, 1060
PsyI GACNNNGTC 1 cut(s) 2233
PteI GCGCGC 1 cut(s) 981
PvuI CGATCG 2 cut(s) 1527, 1710
RruI TCGCGA 2 cut(s) 845, 1523
RsaI GTAC 7 cut(s) 264, 998, 1363, 1577, 1610, 1751, 2171
RsaNI GTAC 7 cut(s) 263, 997, 1362, 1576, 1609, 1750, 2170
RseI CAYNNNNRTG 1 cut(s) 1728
Rsr2I CGGWCCG 1 cut(s) 788
RsrII CGGWCCG 1 cut(s) 788
SacI GAGCTC 3 cut(s) 591, 2223, 2270
SacII CCGCGG 2 cut(s) 571, 1194
SalI GTCGAC 1 cut(s) 362
SapI GCTCTTC 1 cut(s) 1583
SaqAI TTAA 2 cut(s) 272, 932
Sau96I GGNCC 8 cut(s) 159, 489, 716, 788, 885, 1121, 2128, 2398
SchI GAGTC 2 cut(s) 1255, 2001
ScrFI CCNGG 8 cut(s) 556, 1251, 1460, 1481, 1637, 2123, 2216, 2261
SduI GDGCHC 6 cut(s) 591, 1623, 2071, 2122, 2223, 2270
SexAI ACCWGGT 1 cut(s) 2259
SfaNI GCATC 5 cut(s) 73, 696, 1343, 1446, 1732
SfoI GGCGCC 1 cut(s) 1761
Sfr303I CCGCGG 2 cut(s) 571, 1194
SfuI TTCGAA 1 cut(s) 1112
SgrBI CCGCGG 2 cut(s) 571, 1194
SinI GGWCC 5 cut(s) 159, 489, 788, 885, 2398
SmiMI CAYNNNNRTG 1 cut(s) 1728
SphI GCATGC 2 cut(s) 2345, 2388
SspDI GGCGCC 1 cut(s) 1759
SspMI CTAG 1 cut(s) 599
SstI GAGCTC 3 cut(s) 591, 2223, 2270
StyD4I CCNGG 8 cut(s) 554, 1249, 1458, 1479, 1635, 2121, 2214, 2259
StyI CCWWGG 2 cut(s) 1117, 2414
TaaI ACNGT 5 cut(s) 729, 1051, 1943, 2117, 2428
TaiI ACGT 5 cut(s) 13, 25, 1581, 2169, 2237
TaqII GACCGA 1 cut(s) 1823
TatI WGTACW 3 cut(s) 262, 1608, 2169
TauI GCSGC 8 cut(s) 486, 573, 615, 835, 1086, 1286, 1386, 1694
Tru1I TTAA 2 cut(s) 272, 932
Tru9I TTAA 2 cut(s) 272, 932
TscAI CASTG 3 cut(s) 1789, 1819, 2395
TseFI GTSAC 3 cut(s) 1784, 1943, 2134
TseI GCWGC 6 cut(s) 213, 829, 1920, 2142, 2329, 2363
Tsp45I GTSAC 3 cut(s) 1784, 1943, 2134
TspDTI ATGAA 6 cut(s) 521, 908, 1780, 2047, 2307, 2435
TspGWI ACGGA 2 cut(s) 876, 1365
TspRI CASTG 3 cut(s) 1789, 1819, 2395
Tth111I GACNNNGTC 1 cut(s) 2233
Van91I CCANNNNNTGG 1 cut(s) 316
VpaK11BI GGWCC 5 cut(s) 159, 489, 788, 885, 2398
XapI RAATTY 6 cut(s) 34, 173, 867, 879, 1204, 1376
XceI RCATGY 4 cut(s) 263, 2083, 2345, 2388
XcmI CCANNNNNNNNNTGG 1 cut(s) 1132
XmiI GTMKAC 2 cut(s) 363, 2316
XspI CTAG 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.