pycom13g12200

tRNA pseudouridine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006793
Physical Location & Seq
Forward (+)
8424664 .. 8425415
752 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g12200.1

Sequence Viewer

Length: 483 bp
ATGGATCCATTTCAGCGTCACTATGCTTACCATAGTGCTCATAAGCTAAACACCGCTGTTATGCAAGAAGCTGCAAAGTATTTTATTGGAAAACATGATTTCTCTGCTTTTGTCAATGCCTCCCAGAAGGAAAGAGTGCGTGATCCAGTGAAGGATATATTCCGTTTTGATGTCATTGAAATGGGAGCTCTTTTACAGCTAGAAGTTGAAGGCTCCGGCTTCTTATACAGACAAGTCCGGAACATGGTTGCTCTGCTGATTCAAATTGGAAGGGAAGCGCTTCCTCCTGATATTGTTCCCAAGATATTGGCAACACGAGATCGGAGGGTGCTTGCCAAATATACCATGTTTACTCCACCTCATGGGCTTTGTCTTGTGACGGTCAAGTATAACGAGGAGCATCTACGGCTTCCATCCAGTTGCTCCGCAACCAGTTTTGGTAGGCATTATACTATCGGCTCATGTAAGCTTCCATTCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PseudoU_synth_1

Protein Analysis

161

Amino Acids

18.35

Weight (kDa)

9.32

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_1 PF01416 24 - 131 1.7e-26 tRNA pseudouridine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 362
AccIII TCCGGA 1 cut(s) 237
AciI CCGC 2 cut(s) 54, 426
AclWI GGATC 2 cut(s) 12, 137
AfeI AGCGCT 1 cut(s) 279
AfiI CCNNNNNNNGG 2 cut(s) 244, 362
AgsI TTSAA 3 cut(s) 179, 209, 263
AluBI AGCT 5 cut(s) 46, 71, 188, 199, 469
AluI AGCT 5 cut(s) 46, 71, 188, 199, 469
Alw21I GWGCWC 2 cut(s) 40, 190
AlwI GGATC 2 cut(s) 12, 137
Aor13HI TCCGGA 1 cut(s) 237
Aor51HI AGCGCT 1 cut(s) 279
ApeKI GCWGC 1 cut(s) 71
Asp700I GAANNNNTTC 1 cut(s) 279
AspLEI GCGC 1 cut(s) 280
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 190
BauI CACGAG 1 cut(s) 315
Bbv12I GWGCWC 2 cut(s) 40, 190
BbvI GCAGC 1 cut(s) 58
BccI CCATC 1 cut(s) 421
BceAI ACGGC 1 cut(s) 422
BfaI CTAG 1 cut(s) 200
BfoI RGCGCY 1 cut(s) 281
BisI GCNGC 1 cut(s) 72
BlsI GCNGC 1 cut(s) 73
BmiI GGNNCC 2 cut(s) 6, 214
BmsI GCATC 1 cut(s) 409
BsaWI WCCGGW 1 cut(s) 237
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 362
Bse1I ACTGG 3 cut(s) 146, 417, 432
BseAI TCCGGA 1 cut(s) 237
BseGI GGATG 1 cut(s) 413
BseLI CCNNNNNNNGG 2 cut(s) 244, 362
BseNI ACTGG 3 cut(s) 146, 417, 432
BseRI GAGGAG 1 cut(s) 410
BseXI GCAGC 1 cut(s) 58
BsiHKAI GWGCWC 2 cut(s) 40, 190
BsiSI CCGG 2 cut(s) 216, 238
BslI CCNNNNNNNGG 2 cut(s) 244, 362
Bsp1286I GDGCHC 2 cut(s) 40, 190
Bsp13I TCCGGA 1 cut(s) 237
Bsp143I GATC 3 cut(s) 4, 142, 319
BspACI CCGC 2 cut(s) 54, 426
BspEI TCCGGA 1 cut(s) 237
BspLI GGNNCC 2 cut(s) 6, 214
BspPI GGATC 2 cut(s) 12, 137
BsrI ACTGG 3 cut(s) 146, 417, 432
BssMI GATC 3 cut(s) 4, 142, 319
BssSI CACGAG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 382
BstC8I GCNNGC 1 cut(s) 333
BstF5I GGATG 1 cut(s) 413
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 1 cut(s) 280
BstKTI GATC 3 cut(s) 7, 145, 322
BstMBI GATC 3 cut(s) 4, 142, 319
BstMWI GCNNNNNNNGC 1 cut(s) 406
BstV1I GCAGC 1 cut(s) 58
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 307
BstYI RGATCY 1 cut(s) 4
BtsCI GGATG 1 cut(s) 413
BtsIMutI CAGTG 1 cut(s) 153
Cac8I GCNNGC 1 cut(s) 333
CfoI GCGC 1 cut(s) 280
CseI GACGC 1 cut(s) 5
CviAII CATG 5 cut(s) 95, 244, 346, 362, 462
DpnI GATC 3 cut(s) 6, 144, 321
DpnII GATC 3 cut(s) 4, 142, 319
Ecl136II GAGCTC 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 190
Eco47III AGCGCT 1 cut(s) 279
Eco53kI GAGCTC 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 188
EcoT38I GRGCYC 1 cut(s) 190
FaeI CATG 5 cut(s) 98, 247, 349, 365, 465
FatI CATG 5 cut(s) 94, 243, 345, 361, 461
Fnu4HI GCNGC 1 cut(s) 72
FokI GGATG 1 cut(s) 400
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 1 cut(s) 72
FspBI CTAG 1 cut(s) 200
GlaI GCGC 1 cut(s) 279
GluI GCNGC 1 cut(s) 72
HaeII RGCGCY 1 cut(s) 281
HapII CCGG 2 cut(s) 216, 238
HgaI GACGC 1 cut(s) 5
HhaI GCGC 1 cut(s) 280
Hin1II CATG 5 cut(s) 98, 247, 349, 365, 465
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 1 cut(s) 259
HpaII CCGG 2 cut(s) 216, 238
Hpy166II GTNNAC 1 cut(s) 351
Hpy188I TCNGA 1 cut(s) 324
Hpy188III TCNNGA 2 cut(s) 238, 287
Hpy8I GTNNAC 1 cut(s) 351
HpyAV CCTTC 4 cut(s) 121, 145, 203, 264
HpyCH4III ACNGT 1 cut(s) 382
HpyCH4V TGCA 2 cut(s) 64, 74
HpyF10VI GCNNNNNNNGC 1 cut(s) 406
Hsp92II CATG 5 cut(s) 98, 247, 349, 365, 465
HspAI GCGC 1 cut(s) 278
Kpn2I TCCGGA 1 cut(s) 237
Kzo9I GATC 3 cut(s) 4, 142, 319
LmnI GCTCC 4 cut(s) 185, 218, 397, 428
LpnPI CCDG 7 cut(s) 137, 159, 229, 251, 300, 430, 445
Lsp1109I GCAGC 1 cut(s) 58
LweI GCATC 1 cut(s) 409
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 2 cut(s) 17, 376
MalI GATC 3 cut(s) 6, 144, 321
MboI GATC 3 cut(s) 4, 142, 319
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 40, 190
MluCI AATT 1 cut(s) 264
MnlI CCTC 5 cut(s) 130, 294, 318, 369, 388
MroI TCCGGA 1 cut(s) 237
MroXI GAANNNNTTC 1 cut(s) 279
MslI CAYNNNNRTG 1 cut(s) 179
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 2 cut(s) 216, 238
MwoI GCNNNNNNNGC 1 cut(s) 406
NdeII GATC 3 cut(s) 4, 142, 319
NlaIII CATG 5 cut(s) 98, 247, 349, 365, 465
NlaIV GGNNCC 2 cut(s) 6, 214
NmuCI GTSAC 2 cut(s) 17, 376
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 259
PflMI CCANNNNNTGG 1 cut(s) 362
PkrI GCNGC 1 cut(s) 73
Psp124BI GAGCTC 1 cut(s) 190
PspN4I GGNNCC 2 cut(s) 6, 214
PsuI RGATCY 1 cut(s) 4
RseI CAYNNNNRTG 1 cut(s) 179
SacI GAGCTC 1 cut(s) 190
SatI GCNGC 1 cut(s) 72
Sau3AI GATC 3 cut(s) 4, 142, 319
SduI GDGCHC 2 cut(s) 40, 190
SetI ASST 6 cut(s) 48, 73, 190, 201, 361, 471
SfaNI GCATC 1 cut(s) 409
SmiMI CAYNNNNRTG 1 cut(s) 179
Sse9I AATT 1 cut(s) 264
SsiI CCGC 2 cut(s) 54, 426
SspMI CTAG 1 cut(s) 200
SstI GAGCTC 1 cut(s) 190
TaaI ACNGT 1 cut(s) 382
TasI AATT 1 cut(s) 264
TfiI GAWTC 1 cut(s) 259
TscAI CASTG 1 cut(s) 153
TseFI GTSAC 2 cut(s) 17, 376
TseI GCWGC 1 cut(s) 71
Tsp45I GTSAC 2 cut(s) 17, 376
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 1 cut(s) 153
Van91I CCANNNNNTGG 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.