Rh4CG297100

tRNA pseudouridine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0006793
Physical Location & Seq
Forward (+)
55640389 .. 55642462
2074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG297100.1

Sequence Viewer

Length: 987 bp
ATGAGTAGCACTAGTGTATTAGTTGCAGGCCTTCCCTGCCATCCAAACCTTAACGCCACTCTTTCTTCTCCAACTCCAACTCCAACTAATACACCAAACGCAAAGGATGGAGACAATTTGGTAATGAAGATTGATGGGTTTAAGTGGCGTTTGGTTATAGCTTACGACGGCACCCGTTATTCAGGATGGCAATATCAGAGTTCACCACCTACCGTACAGCGCTATGTGGAGAAAGCTCTAATGGGAGCAACGAAGCTGGAAAGGAAGGATCTTCATTTGGTTGGTGCAAGTAGGACAGATAAGGGTGTGCATGCTTGGGGTCAGGTTGCACACTTTGTCACACCGTTCAACTATGACAGAATAGAAAGCATTCATGCAGCTCTCAATGGTTCCCTTCCGGAGGATATCCGTGTTAGGGAAATTAGTCCTGCTGTGCCTGAATTCCATGCTCGTTTTTCAGCCCAAAGGAAGGTGTATCATTACAGAATATATAATGACACATTCATGGACCCATTTCAGCGTCACAATGCTTACCATAGTGCTCATAAGTTGAACCCTGTTGTTATGAGAGAAGCTGCGAAGTATTTTATTGGAAAGCATGATTTCTCTGCTTTTGTAAATGCATCCCAGAAGGAGCGAGTGCGGGATCCAGTAAAGGATATATTCCATTTTGATGTTGTCCAAATGGGGGCTCTTTTACAGCTTGAAGTTGAAGGCTCGGGATTCTTATATAGACAAGTCCGGAACATGGTCGCTCTGCTACTTCAAATCGGAAGGGAAGCAGTTCCCCCTGACATCATACCCAAGATATTGGCAACACGAAATCGAAGGGAGCTTGCTAAATATTCCTTGTTGGCTCCACCCCAAGGGCTTTGTCTTATGGCAGTCGAGTATAACGAGGACCATCTACGGCTTCCACCTGGTTCCCCTAACCCCAGTTTTGGTAGGCATTATACTATAAAATCTTGTAAGCTTCCATTCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PseudoU_synth_1

Protein Analysis

328

Amino Acids

37.14

Weight (kDa)

9.43

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_1 PF01416 53 - 152 8.9e-09 tRNA pseudouridine synthase
PseudoU_synth_1 PF01416 192 - 299 1.5e-26 tRNA pseudouridine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 170
AccIII TCCGGA 2 cut(s) 397, 741
AciI CCGC 1 cut(s) 643
AclWI GGATC 3 cut(s) 276, 641, 654
AcsI RAATTY 1 cut(s) 440
AfaI GTAC 1 cut(s) 216
AfeI AGCGCT 1 cut(s) 221
AfiI CCNNNNNNNGG 7 cut(s) 400, 415, 469, 688, 748, 866, 941
AgsI TTSAA 5 cut(s) 349, 553, 707, 713, 767
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 1 cut(s) 919
AluBI AGCT 8 cut(s) 161, 236, 256, 380, 575, 703, 835, 973
AluI AGCT 8 cut(s) 161, 236, 256, 380, 575, 703, 835, 973
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 1 cut(s) 105
AlwI GGATC 3 cut(s) 276, 641, 654
Ama87I CYCGRG 1 cut(s) 718
Aor13HI TCCGGA 2 cut(s) 397, 741
Aor51HI AGCGCT 1 cut(s) 221
AoxI GGCC 1 cut(s) 28
ApeKI GCWGC 2 cut(s) 377, 575
ApoI RAATTY 1 cut(s) 440
Asp700I GAANNNNTTC 2 cut(s) 369, 783
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 2 cut(s) 508, 901
AsuHPI GGTGA 1 cut(s) 195
AvaI CYCGRG 1 cut(s) 718
AvaII GGWCC 2 cut(s) 508, 901
BamHI GGATCC 1 cut(s) 646
BanI GGYRCC 1 cut(s) 170
BanII GRGCYC 1 cut(s) 694
Bbv12I GWGCWC 1 cut(s) 544
BbvI GCAGC 2 cut(s) 389, 562
BccI CCATC 5 cut(s) 48, 101, 128, 180, 912
BceAI ACGGC 2 cut(s) 184, 926
BciT130I CCWGG 1 cut(s) 921
BcoDI GTCTC 1 cut(s) 105
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 223
BisI GCNGC 2 cut(s) 378, 576
BlsI GCNGC 2 cut(s) 379, 577
Bme1390I CCNGG 1 cut(s) 921
Bme18I GGWCC 2 cut(s) 508, 901
BmeT110I CYCGRG 1 cut(s) 718
BmgT120I GGNCC 2 cut(s) 508, 901
BmiI GGNNCC 6 cut(s) 172, 391, 510, 648, 858, 925
BmrFI CCNGG 1 cut(s) 921
BmrI ACTGGG 1 cut(s) 930
BmsI GCATC 1 cut(s) 632
BmuI ACTGGG 1 cut(s) 930
BsaJI CCNNGG 1 cut(s) 865
BsaWI WCCGGW 2 cut(s) 397, 741
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 7 cut(s) 400, 415, 469, 688, 748, 866, 941
Bse1I ACTGG 2 cut(s) 650, 936
BseAI TCCGGA 2 cut(s) 397, 741
BseBI CCWGG 1 cut(s) 921
BseDI CCNNGG 1 cut(s) 865
BseGI GGATG 4 cut(s) 40, 112, 191, 623
BseLI CCNNNNNNNGG 7 cut(s) 400, 415, 469, 688, 748, 866, 941
BseNI ACTGG 2 cut(s) 650, 936
BseXI GCAGC 2 cut(s) 389, 562
BshFI GGCC 1 cut(s) 30
BshNI GGYRCC 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 544
BsiHKCI CYCGRG 1 cut(s) 718
BsiSI CCGG 2 cut(s) 398, 742
BslI CCNNNNNNNGG 7 cut(s) 400, 415, 469, 688, 748, 866, 941
BsmAI GTCTC 1 cut(s) 105
BsmI GAATGC 1 cut(s) 369
BsnI GGCC 1 cut(s) 30
BsoBI CYCGRG 1 cut(s) 718
Bsp1286I GDGCHC 2 cut(s) 544, 694
Bsp13I TCCGGA 2 cut(s) 397, 741
Bsp143I GATC 2 cut(s) 268, 646
BspACI CCGC 1 cut(s) 643
BspANI GGCC 1 cut(s) 30
BspEI TCCGGA 2 cut(s) 397, 741
BspLI GGNNCC 6 cut(s) 172, 391, 510, 648, 858, 925
BspPI GGATC 3 cut(s) 276, 641, 654
BspT107I GGYRCC 1 cut(s) 170
BsrI ACTGG 2 cut(s) 650, 936
BssECI CCNNGG 1 cut(s) 865
BssMI GATC 2 cut(s) 268, 646
BssT1I CCWWGG 1 cut(s) 865
Bst2UI CCWGG 1 cut(s) 921
Bst4CI ACNGT 2 cut(s) 214, 345
BstC8I GCNNGC 3 cut(s) 28, 312, 837
BstENI CCTNNNNNAGG 1 cut(s) 398
BstF5I GGATG 4 cut(s) 40, 112, 191, 623
BstH2I RGCGCY 1 cut(s) 223
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 2 cut(s) 271, 649
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 2 cut(s) 268, 646
BstMWI GCNNNNNNNGC 1 cut(s) 36
BstNI CCWGG 1 cut(s) 921
BstNSI RCATGY 1 cut(s) 314
BstSCI CCNGG 1 cut(s) 919
BstV1I GCAGC 2 cut(s) 389, 562
BstX2I RGATCY 2 cut(s) 268, 646
BstXI CCANNNNNNTGG 1 cut(s) 811
BstYI RGATCY 2 cut(s) 268, 646
BsuRI GGCC 1 cut(s) 30
BtsCI GGATG 4 cut(s) 40, 112, 191, 623
Cac8I GCNNGC 3 cut(s) 28, 312, 837
CfoI GCGC 1 cut(s) 222
Cfr13I GGNCC 2 cut(s) 508, 901
CseI GACGC 1 cut(s) 509
CsiI ACCWGGT 1 cut(s) 919
Csp6I GTAC 1 cut(s) 215
CviAII CATG 6 cut(s) 311, 374, 446, 505, 599, 748
CviQI GTAC 1 cut(s) 215
DpnI GATC 2 cut(s) 270, 648
DpnII GATC 2 cut(s) 268, 646
Eco130I CCWWGG 1 cut(s) 865
Eco147I AGGCCT 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 694
Eco32I GATATC 1 cut(s) 406
Eco47I GGWCC 2 cut(s) 508, 901
Eco47III AGCGCT 1 cut(s) 221
Eco88I CYCGRG 1 cut(s) 718
EcoNI CCTNNNNNAGG 1 cut(s) 398
EcoRI GAATTC 1 cut(s) 440
EcoRII CCWGG 1 cut(s) 919
EcoRV GATATC 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 865
EcoT22I ATGCAT 1 cut(s) 625
EcoT38I GRGCYC 1 cut(s) 694
ErhI CCWWGG 1 cut(s) 865
FaeI CATG 6 cut(s) 314, 377, 449, 508, 602, 751
FatI CATG 6 cut(s) 310, 373, 445, 504, 598, 747
FauI CCCGC 1 cut(s) 636
Fnu4HI GCNGC 2 cut(s) 378, 576
FokI GGATG 4 cut(s) 27, 119, 198, 610
FriOI GRGCYC 1 cut(s) 694
Fsp4HI GCNGC 2 cut(s) 378, 576
FspBI CTAG 1 cut(s) 12
GlaI GCGC 1 cut(s) 221
GluI GCNGC 2 cut(s) 378, 576
HaeII RGCGCY 1 cut(s) 223
HaeIII GGCC 1 cut(s) 30
HapII CCGG 2 cut(s) 398, 742
HgaI GACGC 1 cut(s) 509
HhaI GCGC 1 cut(s) 222
Hin1II CATG 6 cut(s) 314, 377, 449, 508, 602, 751
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HindIII AAGCTT 1 cut(s) 971
HinfI GANTC 1 cut(s) 723
HpaII CCGG 2 cut(s) 398, 742
HphI GGTGA 1 cut(s) 195
Hpy166II GTNNAC 1 cut(s) 203
Hpy188I TCNGA 2 cut(s) 198, 773
Hpy188III TCNNGA 4 cut(s) 183, 398, 720, 742
Hpy8I GTNNAC 1 cut(s) 203
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 8 cut(s) 41, 259, 404, 463, 625, 707, 768, 822
HpyCH4III ACNGT 2 cut(s) 214, 345
HpyCH4V TGCA 6 cut(s) 26, 287, 310, 329, 377, 623
HpyF10VI GCNNNNNNNGC 1 cut(s) 36
Hsp92II CATG 6 cut(s) 314, 377, 449, 508, 602, 751
HspAI GCGC 1 cut(s) 220
Kpn2I TCCGGA 2 cut(s) 397, 741
Kzo9I GATC 2 cut(s) 268, 646
LmnI GCTCC 4 cut(s) 245, 634, 832, 862
Lsp1109I GCAGC 2 cut(s) 389, 562
LweI GCATC 1 cut(s) 632
MabI ACCWGGT 1 cut(s) 919
MaeI CTAG 1 cut(s) 12
MaeIII GTNAC 2 cut(s) 337, 521
MalI GATC 2 cut(s) 270, 648
MboI GATC 2 cut(s) 268, 646
MboII GAAGA 3 cut(s) 57, 139, 263
MflI RGATCY 2 cut(s) 268, 646
MhlI GDGCHC 2 cut(s) 544, 694
MluCI AATT 3 cut(s) 115, 420, 440
MmeI TCCRAC 3 cut(s) 95, 101, 107
MnlI CCTC 2 cut(s) 394, 892
Mph1103I ATGCAT 1 cut(s) 625
MroI TCCGGA 2 cut(s) 397, 741
MroXI GAANNNNTTC 2 cut(s) 369, 783
MseI TTAA 2 cut(s) 51, 141
MslI CAYNNNNRTG 2 cut(s) 503, 672
MspI CCGG 2 cut(s) 398, 742
MspR9I CCNGG 1 cut(s) 921
Mva1269I GAATGC 1 cut(s) 369
MvaI CCWGG 1 cut(s) 921
MwoI GCNNNNNNNGC 1 cut(s) 36
NdeII GATC 2 cut(s) 268, 646
NlaIII CATG 6 cut(s) 314, 377, 449, 508, 602, 751
NlaIV GGNNCC 6 cut(s) 172, 391, 510, 648, 858, 925
NmuCI GTSAC 2 cut(s) 337, 521
NsiI ATGCAT 1 cut(s) 625
NspI RCATGY 1 cut(s) 314
PaeI GCATGC 1 cut(s) 314
PceI AGGCCT 1 cut(s) 30
PcsI WCGNNNNNNNCGW 1 cut(s) 894
PctI GAATGC 1 cut(s) 369
PdmI GAANNNNTTC 2 cut(s) 369, 783
PfeI GAWTC 1 cut(s) 723
PkrI GCNGC 2 cut(s) 379, 577
Psp6I CCWGG 1 cut(s) 919
PspGI CCWGG 1 cut(s) 919
PspN4I GGNNCC 6 cut(s) 172, 391, 510, 648, 858, 925
PspPI GGNCC 2 cut(s) 508, 901
PsuI RGATCY 2 cut(s) 268, 646
RsaI GTAC 1 cut(s) 216
RsaNI GTAC 1 cut(s) 215
RseI CAYNNNNRTG 2 cut(s) 503, 672
SaqAI TTAA 2 cut(s) 51, 141
SatI GCNGC 2 cut(s) 378, 576
Sau3AI GATC 2 cut(s) 268, 646
Sau96I GGNCC 2 cut(s) 508, 901
ScrFI CCNGG 1 cut(s) 921
SduI GDGCHC 2 cut(s) 544, 694
SexAI ACCWGGT 1 cut(s) 919
SfaNI GCATC 1 cut(s) 632
SinI GGWCC 2 cut(s) 508, 901
SmiMI CAYNNNNRTG 2 cut(s) 503, 672
SpeI ACTAGT 1 cut(s) 11
SphI GCATGC 1 cut(s) 314
Sse9I AATT 3 cut(s) 115, 420, 440
SseBI AGGCCT 1 cut(s) 30
SsiI CCGC 1 cut(s) 643
SspI AATATT 1 cut(s) 845
SspMI CTAG 1 cut(s) 12
StuI AGGCCT 1 cut(s) 30
StyD4I CCNGG 1 cut(s) 919
StyI CCWWGG 1 cut(s) 865
TaaI ACNGT 2 cut(s) 214, 345
TaqI TCGA 2 cut(s) 826, 888
TasI AATT 3 cut(s) 115, 420, 440
TfiI GAWTC 1 cut(s) 723
Tru1I TTAA 2 cut(s) 51, 141
Tru9I TTAA 2 cut(s) 51, 141
TseFI GTSAC 2 cut(s) 337, 521
TseI GCWGC 2 cut(s) 377, 575
Tsp45I GTSAC 2 cut(s) 337, 521
TspDTI ATGAA 4 cut(s) 140, 263, 362, 493
TspGWI ACGGA 1 cut(s) 398
VpaK11BI GGWCC 2 cut(s) 508, 901
XagI CCTNNNNNAGG 1 cut(s) 398
XapI RAATTY 1 cut(s) 440
XceI RCATGY 1 cut(s) 314
XmnI GAANNNNTTC 2 cut(s) 369, 783
XspI CTAG 1 cut(s) 12
Zsp2I ATGCAT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.