Rorug04G0219100

tRNA pseudouridine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006793
Physical Location & Seq
Reverse (-)
37831242 .. 37832601
1360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0219100.1

Sequence Viewer

Length: 792 bp
ATGTCGTCTAGTCCCAACCAGAGTTTTAAGCAGTCCGATGAAACAAAACCCTTAAAGCTTGGGCCGTTCGGATCTGAAAACGGAGGGGAAAGTTTTGATGATGGAGTTCACTCAACGGTCAGGCAGTTGGTGATATCTACAAACGATCAGGCTTCGAGAAATATTGTCTCCATCCAGATTGAATATGACGACAACGGGAGCTCTTTTTTATCAGACAAGCATGGTAAAGAAGACAATTACTATACTTATACGTTCATGGAAAATATGTTAAGCTTTGGCGCTTCTCTTAATTACTGCAAGTGGCTTAGTGGTTCTTGCCTAGTTGGGTGTGCTCCCCAACCTAGGAGCAACAGGAAAACTTACGGACCATATGGAGATGAAGGAAGCCTGGGAAAGTATTTTTCAATCCAAGCGCCTGGGAAAATGATTGTTGGCTTTCATGGTAGGACTTCCATGTTCATGAATCTCCGAGCACTAGGAGCTTACCTAAAGCCTGTCGATCATAATCTGAATGAAACATACAACCCCTCTAAACATCTCGATGATAAAGGATCTTCCATGGTGCCGGCATCACTAGATCCAGCAAATCCTCTACTTCCAGCTACATATCCTTTTCAAACCAAGCTCATAAAGAAACACGAAGATGAAGGTGATAAACATATATCATGCATAATTTCAGGTAATGAAGTTAGCGGAAACGAGGGCAACCGCAGTGGAGGGTTCGCAGTTGGAAACAAGTATATTAATACTTACTACTATTATTCAGAAAAGTTCAAGGCAACGTACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PseudoU_synth_1

Protein Analysis

263

Amino Acids

29.09

Weight (kDa)

6.38

Isoelectric Point (pI)

41.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Jacalin PF01419 20 - 162 6.5e-18 Jacalin-like lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 562
AciI CCGC 2 cut(s) 693, 709
AclWI GGATC 3 cut(s) 79, 559, 572
AfaI GTAC 1 cut(s) 785
AfiI CCNNNNNNNGG 1 cut(s) 342
AgsI TTSAA 4 cut(s) 182, 405, 617, 775
AjnI CCWGG 2 cut(s) 387, 415
AluBI AGCT 6 cut(s) 58, 201, 273, 482, 602, 625
AluI AGCT 6 cut(s) 58, 201, 273, 482, 602, 625
Alw21I GWGCWC 3 cut(s) 203, 334, 475
Alw26I GTCTC 1 cut(s) 172
AlwI GGATC 3 cut(s) 79, 559, 572
AoxI GGCC 1 cut(s) 62
AseI ATTAAT 1 cut(s) 744
AspA2I CCTAGG 1 cut(s) 341
AspLEI GCGC 2 cut(s) 281, 415
AspS9I GGNCC 2 cut(s) 62, 365
AsuHPI GGTGA 2 cut(s) 142, 662
AvaII GGWCC 1 cut(s) 365
AvrII CCTAGG 1 cut(s) 341
BanI GGYRCC 1 cut(s) 562
BanII GRGCYC 1 cut(s) 203
BbsI GAAGAC 1 cut(s) 237
Bbv12I GWGCWC 3 cut(s) 203, 334, 475
BccI CCATC 2 cut(s) 95, 179
BceAI ACGGC 1 cut(s) 49
BciT130I CCWGG 2 cut(s) 389, 417
BcoDI GTCTC 1 cut(s) 172
BfaI CTAG 5 cut(s) 9, 320, 342, 476, 575
BfoI RGCGCY 2 cut(s) 282, 416
BlnI CCTAGG 1 cut(s) 341
Bme1390I CCNGG 2 cut(s) 389, 417
Bme18I GGWCC 1 cut(s) 365
BmgT120I GGNCC 2 cut(s) 62, 365
BmiI GGNNCC 1 cut(s) 564
BmrFI CCNGG 2 cut(s) 389, 417
BmsI GCATC 1 cut(s) 578
BpiI GAAGAC 1 cut(s) 237
BsaBI GATNNNNATC 1 cut(s) 504
BsaJI CCNNGG 4 cut(s) 341, 388, 416, 558
Bsc4I CCNNNNNNNGG 1 cut(s) 342
Bse118I RCCGGY 1 cut(s) 565
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 2 cut(s) 389, 417
BseDI CCNNGG 4 cut(s) 341, 388, 416, 558
BseGI GGATG 1 cut(s) 171
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 1 cut(s) 342
BshFI GGCC 1 cut(s) 64
BshNI GGYRCC 1 cut(s) 562
BsiHKAI GWGCWC 3 cut(s) 203, 334, 475
BsiSI CCGG 1 cut(s) 566
BslI CCNNNNNNNGG 1 cut(s) 342
BsmAI GTCTC 1 cut(s) 172
BsnI GGCC 1 cut(s) 64
Bsp1286I GDGCHC 3 cut(s) 203, 334, 475
Bsp143I GATC 5 cut(s) 71, 145, 499, 551, 577
Bsp19I CCATGG 1 cut(s) 558
BspACI CCGC 2 cut(s) 693, 709
BspANI GGCC 1 cut(s) 64
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 1 cut(s) 564
BspPI GGATC 3 cut(s) 79, 559, 572
BspT107I GGYRCC 1 cut(s) 562
BsrFI RCCGGY 1 cut(s) 565
BssAI RCCGGY 1 cut(s) 565
BssECI CCNNGG 4 cut(s) 341, 388, 416, 558
BssMI GATC 5 cut(s) 71, 145, 499, 551, 577
BssT1I CCWWGG 2 cut(s) 341, 558
Bst2UI CCWGG 2 cut(s) 389, 417
Bst4CI ACNGT 1 cut(s) 118
BstC8I GCNNGC 1 cut(s) 567
BstDEI CTNAG 1 cut(s) 305
BstDSI CCRYGG 1 cut(s) 558
BstF5I GGATG 1 cut(s) 171
BstH2I RGCGCY 2 cut(s) 282, 416
BstHHI GCGC 2 cut(s) 281, 415
BstKTI GATC 5 cut(s) 74, 148, 502, 554, 580
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 5 cut(s) 71, 145, 499, 551, 577
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNI CCWGG 2 cut(s) 389, 417
BstSCI CCNGG 2 cut(s) 387, 415
BstV2I GAAGAC 1 cut(s) 237
BstX2I RGATCY 3 cut(s) 71, 551, 577
BstXI CCANNNNNNTGG 1 cut(s) 416
BstYI RGATCY 3 cut(s) 71, 551, 577
BsuRI GGCC 1 cut(s) 64
BtgI CCRYGG 1 cut(s) 558
BtsCI GGATG 1 cut(s) 171
BtsI GCAGTG 1 cut(s) 718
BtsIMutI CAGTG 1 cut(s) 718
Cac8I GCNNGC 1 cut(s) 567
CciI TCATGA 1 cut(s) 459
CfoI GCGC 2 cut(s) 281, 415
Cfr10I RCCGGY 1 cut(s) 565
Cfr13I GGNCC 2 cut(s) 62, 365
Csp6I GTAC 1 cut(s) 784
CspCI CAANNNNNGTGG 2 cut(s) 694, 729
CviAII CATG 7 cut(s) 221, 256, 440, 454, 460, 559, 666
CviQI GTAC 1 cut(s) 784
DdeI CTNAG 1 cut(s) 305
DpnI GATC 5 cut(s) 73, 147, 501, 553, 579
DpnII GATC 5 cut(s) 71, 145, 499, 551, 577
Ecl136II GAGCTC 1 cut(s) 201
Eco130I CCWWGG 2 cut(s) 341, 558
Eco24I GRGCYC 1 cut(s) 203
Eco32I GATATC 1 cut(s) 135
Eco47I GGWCC 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 201
EcoICRI GAGCTC 1 cut(s) 201
EcoRII CCWGG 2 cut(s) 387, 415
EcoRV GATATC 1 cut(s) 135
EcoT14I CCWWGG 2 cut(s) 341, 558
EcoT22I ATGCAT 1 cut(s) 671
EcoT38I GRGCYC 1 cut(s) 203
ErhI CCWWGG 2 cut(s) 341, 558
FaeI CATG 7 cut(s) 224, 259, 443, 457, 463, 562, 669
FatI CATG 7 cut(s) 220, 255, 439, 453, 459, 558, 665
FauNDI CATATG 1 cut(s) 370
FokI GGATG 1 cut(s) 158
FriOI GRGCYC 1 cut(s) 203
FspBI CTAG 5 cut(s) 9, 320, 342, 476, 575
GlaI GCGC 2 cut(s) 280, 414
HaeII RGCGCY 2 cut(s) 282, 416
HaeIII GGCC 1 cut(s) 64
HapII CCGG 1 cut(s) 566
HhaI GCGC 2 cut(s) 281, 415
Hin1II CATG 7 cut(s) 224, 259, 443, 457, 463, 562, 669
Hin6I GCGC 2 cut(s) 279, 413
HinP1I GCGC 2 cut(s) 279, 413
HindIII AAGCTT 2 cut(s) 56, 271
HinfI GANTC 1 cut(s) 463
HpaII CCGG 1 cut(s) 566
HphI GGTGA 2 cut(s) 142, 662
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 7 cut(s) 37, 71, 76, 214, 470, 510, 766
Hpy188III TCNNGA 4 cut(s) 156, 175, 460, 539
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 2 cut(s) 374, 641
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4IV ACGT 2 cut(s) 251, 782
HpyCH4V TGCA 2 cut(s) 297, 669
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpyF3I CTNAG 1 cut(s) 305
HpySE526I ACGT 2 cut(s) 251, 782
Hsp92II CATG 7 cut(s) 224, 259, 443, 457, 463, 562, 669
HspAI GCGC 2 cut(s) 279, 413
KroI GCCGGC 1 cut(s) 565
KroNI GCCGGC 1 cut(s) 567
Kzo9I GATC 5 cut(s) 71, 145, 499, 551, 577
LmnI GCTCC 4 cut(s) 198, 337, 345, 479
LweI GCATC 1 cut(s) 578
MaeI CTAG 5 cut(s) 9, 320, 342, 476, 575
MaeII ACGT 2 cut(s) 251, 782
MalI GATC 5 cut(s) 73, 147, 501, 553, 579
MboI GATC 5 cut(s) 71, 145, 499, 551, 577
MboII GAAGA 3 cut(s) 242, 546, 653
MfeI CAATTG 1 cut(s) 787
MflI RGATCY 3 cut(s) 71, 551, 577
MhlI GDGCHC 3 cut(s) 203, 334, 475
MluCI AATT 4 cut(s) 235, 289, 672, 787
MmeI TCCRAC 1 cut(s) 709
MnlI CCTC 5 cut(s) 77, 538, 600, 694, 710
Mph1103I ATGCAT 1 cut(s) 671
MroNI GCCGGC 1 cut(s) 565
MseI TTAA 5 cut(s) 27, 53, 269, 288, 744
MslI CAYNNNNRTG 3 cut(s) 458, 540, 642
MspI CCGG 1 cut(s) 566
MspR9I CCNGG 2 cut(s) 389, 417
MunI CAATTG 1 cut(s) 787
MvaI CCWGG 2 cut(s) 389, 417
MwoI GCNNNNNNNGC 1 cut(s) 479
NaeI GCCGGC 1 cut(s) 567
NcoI CCATGG 1 cut(s) 558
NdeI CATATG 1 cut(s) 370
NdeII GATC 5 cut(s) 71, 145, 499, 551, 577
NgoMIV GCCGGC 1 cut(s) 565
NlaIII CATG 7 cut(s) 224, 259, 443, 457, 463, 562, 669
NlaIV GGNNCC 1 cut(s) 564
NsiI ATGCAT 1 cut(s) 671
PagI TCATGA 1 cut(s) 459
PdiI GCCGGC 1 cut(s) 567
PfeI GAWTC 1 cut(s) 463
PshBI ATTAAT 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 203
Psp6I CCWGG 2 cut(s) 387, 415
PspGI CCWGG 2 cut(s) 387, 415
PspN4I GGNNCC 1 cut(s) 564
PspPI GGNCC 2 cut(s) 62, 365
PsuI RGATCY 3 cut(s) 71, 551, 577
RsaI GTAC 1 cut(s) 785
RsaNI GTAC 1 cut(s) 784
RseI CAYNNNNRTG 3 cut(s) 458, 540, 642
SacI GAGCTC 1 cut(s) 203
SaqAI TTAA 5 cut(s) 27, 53, 269, 288, 744
Sau3AI GATC 5 cut(s) 71, 145, 499, 551, 577
Sau96I GGNCC 2 cut(s) 62, 365
ScrFI CCNGG 2 cut(s) 389, 417
SduI GDGCHC 3 cut(s) 203, 334, 475
SfaNI GCATC 1 cut(s) 578
SinI GGWCC 1 cut(s) 365
SmiMI CAYNNNNRTG 3 cut(s) 458, 540, 642
Sse9I AATT 4 cut(s) 235, 289, 672, 787
SsiI CCGC 2 cut(s) 693, 709
SspI AATATT 1 cut(s) 163
SspMI CTAG 5 cut(s) 9, 320, 342, 476, 575
SstI GAGCTC 1 cut(s) 203
StyD4I CCNGG 2 cut(s) 387, 415
StyI CCWWGG 2 cut(s) 341, 558
TaaI ACNGT 1 cut(s) 118
TaiI ACGT 2 cut(s) 254, 785
TaqI TCGA 3 cut(s) 155, 498, 540
TasI AATT 4 cut(s) 235, 289, 672, 787
TfiI GAWTC 1 cut(s) 463
Tru1I TTAA 5 cut(s) 27, 53, 269, 288, 744
Tru9I TTAA 5 cut(s) 27, 53, 269, 288, 744
TscAI CASTG 1 cut(s) 718
TspDTI ATGAA 9 cut(s) 54, 244, 393, 428, 448, 476, 528, 660, 699
TspGWI ACGGA 2 cut(s) 96, 378
TspRI CASTG 1 cut(s) 718
VpaK11BI GGWCC 1 cut(s) 365
VspI ATTAAT 1 cut(s) 744
XmaJI CCTAGG 1 cut(s) 341
XspI CTAG 5 cut(s) 9, 320, 342, 476, 575
Zsp2I ATGCAT 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.