Rmu_sc0016038.1_g000001

tRNA pseudouridine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0016038.1
OG0006793
Physical Location & Seq
Forward (+)
1 .. 981
981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0016038.1_g000001.1.cds

Sequence Viewer

Length: 642 bp
ttcaactatgacagaatagaaagcattcatgcagctctcaatggtactcttccggaggatatccgtgttagggaaattagtcctgctgtgcctgaattccatgctcgtttttcagcccaaaggaaggtgtatcattacagaatatataatgacacattcatggacccatttcagcgtcacaatgcttaccatagtgctcataagttgaaccctgctgttatgagagaagctgcgaagtattttattggaaagcatgatttctctgcttttgtaaatgcatcccagaaggagcgagtgcgggatccagtaaaggatatattccgttttgatgttgtccaaatgggggctcttttacagcttgaagttgaaggctcgggattcttatatagacaagtccggaacatggtcgctctgctacttcaaatcggaagggaagcagttccccctgacatcatacccaagatattggcaacacgaaatcgaagggagcttgctaaatattccttgttggctccaccccaagggctttgtcttatggcagtcgagtataacgaggaccatctacggcttccacctggttcccctaaccccagttttggtaggcattatactataaaatcttgtaagcttccattctactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PseudoU_synth_1

Protein Analysis

213

Amino Acids

24.61

Weight (kDa)

9.44

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 52, 396
AciI CCGC 1 cut(s) 298
AclWI GGATC 2 cut(s) 296, 309
AcsI RAATTY 1 cut(s) 95
AfaI GTAC 1 cut(s) 46
AfiI CCNNNNNNNGG 6 cut(s) 70, 124, 343, 403, 521, 596
AgsI TTSAA 5 cut(s) 4, 208, 362, 368, 422
AjnI CCWGG 1 cut(s) 574
AluBI AGCT 5 cut(s) 35, 230, 358, 490, 628
AluI AGCT 5 cut(s) 35, 230, 358, 490, 628
Alw21I GWGCWC 1 cut(s) 199
AlwI GGATC 2 cut(s) 296, 309
Ama87I CYCGRG 1 cut(s) 373
Aor13HI TCCGGA 2 cut(s) 52, 396
ApeKI GCWGC 2 cut(s) 32, 230
ApoI RAATTY 1 cut(s) 95
Asp700I GAANNNNTTC 2 cut(s) 24, 438
AspS9I GGNCC 2 cut(s) 163, 556
AvaI CYCGRG 1 cut(s) 373
AvaII GGWCC 2 cut(s) 163, 556
BamHI GGATCC 1 cut(s) 301
BanII GRGCYC 1 cut(s) 349
Bbv12I GWGCWC 1 cut(s) 199
BbvI GCAGC 2 cut(s) 44, 217
BccI CCATC 1 cut(s) 567
BceAI ACGGC 1 cut(s) 581
BciT130I CCWGG 1 cut(s) 576
BisI GCNGC 2 cut(s) 33, 231
BlsI GCNGC 2 cut(s) 34, 232
Bme1390I CCNGG 1 cut(s) 576
Bme18I GGWCC 2 cut(s) 163, 556
BmeT110I CYCGRG 1 cut(s) 373
BmgT120I GGNCC 2 cut(s) 163, 556
BmiI GGNNCC 4 cut(s) 165, 303, 513, 580
BmrFI CCNGG 1 cut(s) 576
BmrI ACTGGG 1 cut(s) 585
BmsI GCATC 1 cut(s) 287
BmuI ACTGGG 1 cut(s) 585
BsaJI CCNNGG 1 cut(s) 520
BsaWI WCCGGW 2 cut(s) 52, 396
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 124, 343, 403, 521, 596
Bse1I ACTGG 2 cut(s) 305, 591
BseAI TCCGGA 2 cut(s) 52, 396
BseBI CCWGG 1 cut(s) 576
BseDI CCNNGG 1 cut(s) 520
BseGI GGATG 1 cut(s) 278
BseLI CCNNNNNNNGG 6 cut(s) 70, 124, 343, 403, 521, 596
BseNI ACTGG 2 cut(s) 305, 591
BseXI GCAGC 2 cut(s) 44, 217
BsiHKAI GWGCWC 1 cut(s) 199
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 2 cut(s) 53, 397
BslI CCNNNNNNNGG 6 cut(s) 70, 124, 343, 403, 521, 596
BsmI GAATGC 1 cut(s) 24
BsoBI CYCGRG 1 cut(s) 373
Bsp1286I GDGCHC 2 cut(s) 199, 349
Bsp13I TCCGGA 2 cut(s) 52, 396
Bsp143I GATC 1 cut(s) 301
BspACI CCGC 1 cut(s) 298
BspEI TCCGGA 2 cut(s) 52, 396
BspLI GGNNCC 4 cut(s) 165, 303, 513, 580
BspPI GGATC 2 cut(s) 296, 309
BsrI ACTGG 2 cut(s) 305, 591
BssECI CCNNGG 1 cut(s) 520
BssMI GATC 1 cut(s) 301
BssT1I CCWWGG 1 cut(s) 520
Bst2UI CCWGG 1 cut(s) 576
Bst6I CTCTTC 1 cut(s) 54
BstC8I GCNNGC 1 cut(s) 492
BstF5I GGATG 1 cut(s) 278
BstKTI GATC 1 cut(s) 304
BstMBI GATC 1 cut(s) 301
BstNI CCWGG 1 cut(s) 576
BstSCI CCNGG 1 cut(s) 574
BstV1I GCAGC 2 cut(s) 44, 217
BstX2I RGATCY 1 cut(s) 301
BstXI CCANNNNNNTGG 1 cut(s) 466
BstYI RGATCY 1 cut(s) 301
BtsCI GGATG 1 cut(s) 278
Cac8I GCNNGC 1 cut(s) 492
Cfr13I GGNCC 2 cut(s) 163, 556
CseI GACGC 1 cut(s) 164
CsiI ACCWGGT 1 cut(s) 574
Csp6I GTAC 1 cut(s) 45
CviAII CATG 5 cut(s) 29, 101, 160, 254, 403
CviQI GTAC 1 cut(s) 45
DpnI GATC 1 cut(s) 303
DpnII GATC 1 cut(s) 301
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 520
Eco24I GRGCYC 1 cut(s) 349
Eco32I GATATC 1 cut(s) 61
Eco47I GGWCC 2 cut(s) 163, 556
Eco88I CYCGRG 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 574
EcoRV GATATC 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 520
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 520
FaeI CATG 5 cut(s) 32, 104, 163, 257, 406
FatI CATG 5 cut(s) 28, 100, 159, 253, 402
FauI CCCGC 1 cut(s) 291
Fnu4HI GCNGC 2 cut(s) 33, 231
FokI GGATG 1 cut(s) 265
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 2 cut(s) 33, 231
GluI GCNGC 2 cut(s) 33, 231
HapII CCGG 2 cut(s) 53, 397
HgaI GACGC 1 cut(s) 164
Hin1II CATG 5 cut(s) 32, 104, 163, 257, 406
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 1 cut(s) 378
HpaII CCGG 2 cut(s) 53, 397
Hpy188I TCNGA 1 cut(s) 428
Hpy188III TCNNGA 3 cut(s) 53, 375, 397
HpyAV CCTTC 5 cut(s) 118, 280, 362, 423, 477
HpyCH4V TGCA 2 cut(s) 32, 278
Hsp92II CATG 5 cut(s) 32, 104, 163, 257, 406
Kpn2I TCCGGA 2 cut(s) 52, 396
Kzo9I GATC 1 cut(s) 301
LmnI GCTCC 3 cut(s) 289, 487, 517
Lsp1109I GCAGC 2 cut(s) 44, 217
LweI GCATC 1 cut(s) 287
MabI ACCWGGT 1 cut(s) 574
MaeIII GTNAC 1 cut(s) 176
MalI GATC 1 cut(s) 303
MboI GATC 1 cut(s) 301
MboII GAAGA 1 cut(s) 41
MflI RGATCY 1 cut(s) 301
MhlI GDGCHC 2 cut(s) 199, 349
MluCI AATT 2 cut(s) 75, 95
MnlI CCTC 2 cut(s) 49, 547
Mph1103I ATGCAT 1 cut(s) 280
MroI TCCGGA 2 cut(s) 52, 396
MroXI GAANNNNTTC 2 cut(s) 24, 438
MslI CAYNNNNRTG 1 cut(s) 158
MspI CCGG 2 cut(s) 53, 397
MspR9I CCNGG 1 cut(s) 576
Mva1269I GAATGC 1 cut(s) 24
MvaI CCWGG 1 cut(s) 576
NdeII GATC 1 cut(s) 301
NlaIII CATG 5 cut(s) 32, 104, 163, 257, 406
NlaIV GGNNCC 4 cut(s) 165, 303, 513, 580
NmuCI GTSAC 1 cut(s) 176
NsiI ATGCAT 1 cut(s) 280
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PctI GAATGC 1 cut(s) 24
PdmI GAANNNNTTC 2 cut(s) 24, 438
PfeI GAWTC 1 cut(s) 378
PkrI GCNGC 2 cut(s) 34, 232
Psp6I CCWGG 1 cut(s) 574
PspGI CCWGG 1 cut(s) 574
PspN4I GGNNCC 4 cut(s) 165, 303, 513, 580
PspPI GGNCC 2 cut(s) 163, 556
PsuI RGATCY 1 cut(s) 301
RsaI GTAC 1 cut(s) 46
RsaNI GTAC 1 cut(s) 45
RseI CAYNNNNRTG 1 cut(s) 158
SatI GCNGC 2 cut(s) 33, 231
Sau3AI GATC 1 cut(s) 301
Sau96I GGNCC 2 cut(s) 163, 556
ScrFI CCNGG 1 cut(s) 576
SduI GDGCHC 2 cut(s) 199, 349
SetI ASST 7 cut(s) 37, 129, 232, 360, 492, 577, 630
SexAI ACCWGGT 1 cut(s) 574
SfaNI GCATC 1 cut(s) 287
SinI GGWCC 2 cut(s) 163, 556
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 2 cut(s) 75, 95
SsiI CCGC 1 cut(s) 298
SspI AATATT 1 cut(s) 500
StyD4I CCNGG 1 cut(s) 574
StyI CCWWGG 1 cut(s) 520
TaqI TCGA 2 cut(s) 481, 543
TasI AATT 2 cut(s) 75, 95
TfiI GAWTC 1 cut(s) 378
TseFI GTSAC 1 cut(s) 176
TseI GCWGC 2 cut(s) 32, 230
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 2 cut(s) 17, 148
TspGWI ACGGA 2 cut(s) 53, 311
VpaK11BI GGWCC 2 cut(s) 163, 556
XapI RAATTY 1 cut(s) 95
XmnI GAANNNNTTC 2 cut(s) 24, 438
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.