Rorug04G0219200

tRNA pseudouridine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006793
Physical Location & Seq
Reverse (-)
37837644 .. 37839002
1359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0219200.1

Sequence Viewer

Length: 756 bp
ATGAGTTTGAAGGATTCTGGTGAGAAAAAACTGTTGAAACTTGGGCCCTTTGGATCAGAAACCGGAGGGGAAAGTTTTGATGATGGAGCTCATTCAACAGTCAGGCAATTGGTAATATCTCATTCATCGAACATTGACTCCATACAGATTGAATATGATGACAACGGGAGCTCATTTTGGTCAGACAAGCATGGTCATGAAGGCGATACCTATATTGACACGATCAAACTTGATTATCCGTACGAATATTTGATTTCAGTTCATGGAAAATACGTTAAGTTTTGGCGGTTCCCAACCTATATTTGCTCCCTTACTTTTAGGAGCAACAAGAAATGTTACGGACCATATGGAGATGAAGGAAACCTGGGAAAGTATTTTTCAATCCAAGCACCTGGGAATATGATTGTCGGCTTTCATGGTAGGGCTTCCATTTTTATGGATATCCGTGCAATAGGAGCTTACCTTAAACCCCTTGATCATTATCAGAATGAAAAGTACAACCCCTCTAAAGTTCTCGAGGTCGTGCCCGCACTACGAAATCCGTCAAGGCCTCTACGTCAAGCTAGATATCCTTCTCAGACCAAGCTCAGAAACACACACGATGACGACGAAGAAGATAACAAACAATTATCATGCATAATTGCCGGTAACCAAGTTAAGGGAAACGAGGGCGACCGGAATGGAGGATTCGCTGTTGGAAATACATTTATTAATACCCACTACTATTATCCATTAAAGTTCAATAATGCCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PseudoU_synth_1

Protein Analysis

251

Amino Acids

28.42

Weight (kDa)

6.75

Isoelectric Point (pI)

33.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Jacalin PF01419 14 - 154 4.5e-35 Jacalin-like lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 286, 528
AclWI GGATC 1 cut(s) 61
AfaI GTAC 2 cut(s) 242, 497
AfiI CCNNNNNNNGG 1 cut(s) 658
AgsI TTSAA 6 cut(s) 10, 37, 96, 152, 381, 742
AjnI CCWGG 2 cut(s) 363, 391
AloI GAACNNNNNNTCC 2 cut(s) 122, 154
AluBI AGCT 5 cut(s) 89, 171, 458, 563, 586
AluI AGCT 5 cut(s) 89, 171, 458, 563, 586
Alw21I GWGCWC 2 cut(s) 91, 173
AlwI GGATC 1 cut(s) 61
Ama87I CYCGRG 1 cut(s) 515
AoxI GGCC 2 cut(s) 44, 548
ApaI GGGCCC 1 cut(s) 48
AseI ATTAAT 1 cut(s) 711
AspS9I GGNCC 3 cut(s) 44, 45, 341
AsuHPI GGTGA 1 cut(s) 32
AvaI CYCGRG 1 cut(s) 515
AvaII GGWCC 1 cut(s) 341
BaeGI GKGCMC 2 cut(s) 48, 528
BanII GRGCYC 3 cut(s) 48, 91, 173
Bbv12I GWGCWC 2 cut(s) 91, 173
BccI CCATC 1 cut(s) 77
BciT130I CCWGG 2 cut(s) 365, 393
BclI TGATCA 1 cut(s) 475
BfaI CTAG 1 cut(s) 564
Bme1390I CCNGG 2 cut(s) 365, 393
Bme18I GGWCC 1 cut(s) 341
BmeT110I CYCGRG 1 cut(s) 515
BmgT120I GGNCC 3 cut(s) 44, 45, 341
BmiI GGNNCC 2 cut(s) 46, 290
BmrFI CCNGG 2 cut(s) 365, 393
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 2 cut(s) 364, 392
BsaWI WCCGGW 2 cut(s) 62, 675
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 1 cut(s) 658
Bse118I RCCGGY 1 cut(s) 644
Bse8I GATNNNNATC 1 cut(s) 480
BseBI CCWGG 2 cut(s) 365, 393
BseDI CCNNGG 2 cut(s) 364, 392
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 658
BseMII CTCAG 2 cut(s) 590, 601
BseSI GKGCMC 2 cut(s) 48, 528
Bsh1285I CGRYCG 1 cut(s) 676
BshFI GGCC 2 cut(s) 46, 550
BsiEI CGRYCG 1 cut(s) 676
BsiHKAI GWGCWC 2 cut(s) 91, 173
BsiHKCI CYCGRG 1 cut(s) 515
BsiSI CCGG 3 cut(s) 63, 645, 676
BsiWI CGTACG 1 cut(s) 240
BslI CCNNNNNNNGG 1 cut(s) 658
BsnI GGCC 2 cut(s) 46, 550
BsoBI CYCGRG 1 cut(s) 515
Bsp120I GGGCCC 1 cut(s) 44
Bsp1286I GDGCHC 4 cut(s) 48, 91, 173, 528
Bsp143I GATC 3 cut(s) 53, 222, 475
BspACI CCGC 2 cut(s) 286, 528
BspANI GGCC 2 cut(s) 46, 550
BspCNI CTCAG 2 cut(s) 589, 600
BspHI TCATGA 1 cut(s) 196
BspLI GGNNCC 2 cut(s) 46, 290
BspPI GGATC 1 cut(s) 61
BsrFI RCCGGY 1 cut(s) 644
BssAI RCCGGY 1 cut(s) 644
BssECI CCNNGG 2 cut(s) 364, 392
BssMI GATC 3 cut(s) 53, 222, 475
Bst2UI CCWGG 2 cut(s) 365, 393
Bst4CI ACNGT 2 cut(s) 33, 100
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 2 cut(s) 576, 587
BstEII GGTNACC 1 cut(s) 647
BstKTI GATC 3 cut(s) 56, 225, 478
BstMBI GATC 3 cut(s) 53, 222, 475
BstMCI CGRYCG 1 cut(s) 676
BstMWI GCNNNNNNNGC 1 cut(s) 455
BstNI CCWGG 2 cut(s) 365, 393
BstPI GGTNACC 1 cut(s) 647
BstSCI CCNGG 2 cut(s) 363, 391
BstSLI GKGCMC 2 cut(s) 48, 528
BstXI CCANNNNNNTGG 2 cut(s) 392, 436
BsuRI GGCC 2 cut(s) 46, 550
Cac8I GCNNGC 1 cut(s) 528
CciI TCATGA 1 cut(s) 196
Cfr10I RCCGGY 1 cut(s) 644
Cfr13I GGNCC 3 cut(s) 44, 45, 341
Csp6I GTAC 2 cut(s) 241, 496
CviAII CATG 6 cut(s) 191, 197, 263, 416, 633, 751
CviJI RGCY 9 cut(s) 46, 89, 171, 411, 425, 458, 550, 563, 586
CviKI_1 RGCY 9 cut(s) 46, 89, 171, 411, 425, 458, 550, 563, 586
CviQI GTAC 2 cut(s) 241, 496
DdeI CTNAG 2 cut(s) 576, 587
DpnI GATC 3 cut(s) 55, 224, 477
DpnII GATC 3 cut(s) 53, 222, 475
Ecl136II GAGCTC 2 cut(s) 89, 171
Eco147I AGGCCT 1 cut(s) 550
Eco24I GRGCYC 3 cut(s) 48, 91, 173
Eco32I GATATC 2 cut(s) 442, 569
Eco47I GGWCC 1 cut(s) 341
Eco53kI GAGCTC 2 cut(s) 89, 171
Eco88I CYCGRG 1 cut(s) 515
Eco91I GGTNACC 1 cut(s) 647
EcoICRI GAGCTC 2 cut(s) 89, 171
EcoO109I RGGNCCY 1 cut(s) 45
EcoO65I GGTNACC 1 cut(s) 647
EcoRII CCWGG 2 cut(s) 363, 391
EcoRV GATATC 2 cut(s) 442, 569
EcoT22I ATGCAT 1 cut(s) 638
EcoT38I GRGCYC 3 cut(s) 48, 91, 173
FaeI CATG 6 cut(s) 194, 200, 266, 419, 636, 754
FatI CATG 6 cut(s) 190, 196, 262, 415, 632, 750
FauI CCCGC 1 cut(s) 535
FauNDI CATATG 1 cut(s) 346
FbaI TGATCA 1 cut(s) 475
FriOI GRGCYC 3 cut(s) 48, 91, 173
FspBI CTAG 1 cut(s) 564
HaeIII GGCC 2 cut(s) 46, 550
HapII CCGG 3 cut(s) 63, 645, 676
Hin1II CATG 6 cut(s) 194, 200, 266, 419, 636, 754
HinfI GANTC 3 cut(s) 14, 137, 687
HpaII CCGG 3 cut(s) 63, 645, 676
HphI GGTGA 1 cut(s) 32
Hpy188I TCNGA 5 cut(s) 58, 184, 486, 579, 590
Hpy188III TCNNGA 2 cut(s) 197, 515
Hpy99I CGWCG 1 cut(s) 611
HpyAV CCTTC 4 cut(s) 4, 194, 350, 582
HpyCH4III ACNGT 2 cut(s) 33, 100
HpyCH4IV ACGT 2 cut(s) 273, 556
HpyCH4V TGCA 2 cut(s) 449, 636
HpyF10VI GCNNNNNNNGC 1 cut(s) 455
HpyF3I CTNAG 2 cut(s) 576, 587
HpySE526I ACGT 2 cut(s) 273, 556
Hsp92II CATG 6 cut(s) 194, 200, 266, 419, 636, 754
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 3 cut(s) 53, 222, 475
LmnI GCTCC 5 cut(s) 86, 168, 311, 321, 455
LpnPI CCDG 9 cut(s) 3, 76, 88, 350, 377, 378, 405, 658, 689
MaeI CTAG 1 cut(s) 564
MaeII ACGT 2 cut(s) 273, 556
MaeIII GTNAC 2 cut(s) 335, 647
MalI GATC 3 cut(s) 55, 224, 477
MboI GATC 3 cut(s) 53, 222, 475
MboII GAAGA 2 cut(s) 623, 626
MfeI CAATTG 1 cut(s) 107
MhlI GDGCHC 4 cut(s) 48, 91, 173, 528
MluCI AATT 3 cut(s) 107, 626, 639
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 676
MnlI CCTC 6 cut(s) 59, 511, 514, 561, 661, 677
Mph1103I ATGCAT 1 cut(s) 638
MseI TTAA 5 cut(s) 276, 465, 657, 711, 734
MslI CAYNNNNRTG 2 cut(s) 195, 434
MspI CCGG 3 cut(s) 63, 645, 676
MspR9I CCNGG 2 cut(s) 365, 393
MunI CAATTG 1 cut(s) 107
MvaI CCWGG 2 cut(s) 365, 393
MwoI GCNNNNNNNGC 1 cut(s) 455
NdeI CATATG 1 cut(s) 346
NdeII GATC 3 cut(s) 53, 222, 475
NlaIII CATG 6 cut(s) 194, 200, 266, 419, 636, 754
NlaIV GGNNCC 2 cut(s) 46, 290
NsiI ATGCAT 1 cut(s) 638
PaeR7I CTCGAG 1 cut(s) 515
PagI TCATGA 1 cut(s) 196
PceI AGGCCT 1 cut(s) 550
PcsI WCGNNNNNNNCGW 1 cut(s) 606
PfeI GAWTC 2 cut(s) 14, 687
Pfl23II CGTACG 1 cut(s) 240
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PshBI ATTAAT 1 cut(s) 711
Psp124BI GAGCTC 2 cut(s) 91, 173
Psp6I CCWGG 2 cut(s) 363, 391
PspEI GGTNACC 1 cut(s) 647
PspGI CCWGG 2 cut(s) 363, 391
PspLI CGTACG 1 cut(s) 240
PspN4I GGNNCC 2 cut(s) 46, 290
PspOMI GGGCCC 1 cut(s) 44
PspPI GGNCC 3 cut(s) 44, 45, 341
RsaI GTAC 2 cut(s) 242, 497
RsaNI GTAC 2 cut(s) 241, 496
RseI CAYNNNNRTG 2 cut(s) 195, 434
SacI GAGCTC 2 cut(s) 91, 173
SaqAI TTAA 5 cut(s) 276, 465, 657, 711, 734
Sau3AI GATC 3 cut(s) 53, 222, 475
Sau96I GGNCC 3 cut(s) 44, 45, 341
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 2 cut(s) 365, 393
SduI GDGCHC 4 cut(s) 48, 91, 173, 528
Sfr274I CTCGAG 1 cut(s) 515
SinI GGWCC 1 cut(s) 341
SlaI CTCGAG 1 cut(s) 515
SmiMI CAYNNNNRTG 2 cut(s) 195, 434
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
Sse9I AATT 3 cut(s) 107, 626, 639
SseBI AGGCCT 1 cut(s) 550
SsiI CCGC 2 cut(s) 286, 528
SspI AATATT 1 cut(s) 248
SspMI CTAG 1 cut(s) 564
SstI GAGCTC 2 cut(s) 91, 173
StuI AGGCCT 1 cut(s) 550
StyD4I CCNGG 2 cut(s) 363, 391
TaaI ACNGT 2 cut(s) 33, 100
TaiI ACGT 2 cut(s) 276, 559
TaqI TCGA 2 cut(s) 128, 516
TasI AATT 3 cut(s) 107, 626, 639
TatI WGTACW 1 cut(s) 495
TfiI GAWTC 2 cut(s) 14, 687
Tru1I TTAA 5 cut(s) 276, 465, 657, 711, 734
Tru9I TTAA 5 cut(s) 276, 465, 657, 711, 734
TspDTI ATGAA 6 cut(s) 114, 213, 251, 369, 404, 504
TspGWI ACGGA 4 cut(s) 228, 354, 434, 531
VpaK11BI GGWCC 1 cut(s) 341
VspI ATTAAT 1 cut(s) 711
XhoI CTCGAG 1 cut(s) 515
XspI CTAG 1 cut(s) 564
Zsp2I ATGCAT 1 cut(s) 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.