pycom13g12570

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007149
Physical Location & Seq
Forward (+)
8756753 .. 8760634
3882 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g12570.5

Sequence Viewer

Length: 654 bp
ATGGGATTTTCACCATCACTTGTGGACAAAGTGATTGAAGAAAAGGGTGAAGGCGACACAGAGTTGTTATTGGAGACACTTTTGTCGTATCCAGATTCTCGAAAGTCAGATTCTGGATCATCTGATTCTCTGGATAGCTTATTTGATGACAAGGATGTAAGCAGTCATCCAGAGATTCCTGATATTGCTGAACCAAAAGAGGAGCCAGATTTGCCAGATGGACTTAATGAGGAAAAAAGGGCATCCTTACTGATGATGAATTTTTCTCCAAACGAAGTTGAGTTTGCAATTGATAAGCTTGGTGAAGACGCTCCAATTGATGACTTGGTGGATTTTATTTTTGCTGCTCAGACAGCAGTCAAACTTGAAAATGATGTAGATGATACAACTCATGGTGGTGAAGAAAAAAATGAGGATGCTAATGATGAAAAATTATATGGGACCATGGAGAAGACACTCCGTCTGCTTGAAATGGGTTTCTCTGAGAATCAAGTTTCTTGGGTAATTGAAAGGTTTGGTTCAGGAGCTCCAATTTCTGAACTTGCGGACTCAATCATTAAATATCAAATTTCTGGAAACTATTATGAAGAAACTAAGTACTCAGCCCCTTCACGCAGAACAGGTTCCGGATTTTATGCTACAGGATCGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

218

Amino Acids

23.95

Weight (kDa)

4.14

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 82
AccIII TCCGGA 1 cut(s) 626
AciI CCGC 1 cut(s) 545
AclWI GGATC 2 cut(s) 124, 652
AcsI RAATTY 2 cut(s) 259, 567
AfaI GTAC 1 cut(s) 599
AgsI TTSAA 4 cut(s) 38, 368, 470, 509
AhdI GACNNNNNGTC 1 cut(s) 459
AluBI AGCT 3 cut(s) 138, 298, 527
AluI AGCT 3 cut(s) 138, 298, 527
Alw21I GWGCWC 1 cut(s) 529
Alw26I GTCTC 1 cut(s) 68
AlwI GGATC 2 cut(s) 124, 652
AlwNI CAGNNNCTG 1 cut(s) 113
Aor13HI TCCGGA 1 cut(s) 626
ApeKI GCWGC 1 cut(s) 344
ApoI RAATTY 2 cut(s) 259, 567
Asp700I GAANNNNTTC 1 cut(s) 622
AspS9I GGNCC 1 cut(s) 441
AsuHPI GGTGA 4 cut(s) 3, 59, 314, 410
AvaII GGWCC 1 cut(s) 441
BanII GRGCYC 1 cut(s) 529
BbsI GAAGAC 2 cut(s) 312, 458
Bbv12I GWGCWC 1 cut(s) 529
BbvI GCAGC 1 cut(s) 331
BccI CCATC 2 cut(s) 22, 212
BciVI GTATCC 1 cut(s) 99
BcoDI GTCTC 1 cut(s) 68
BfmI CTRYAG 1 cut(s) 639
BfuI GTATCC 1 cut(s) 99
BisI GCNGC 1 cut(s) 345
BlsI GCNGC 1 cut(s) 346
BmcAI AGTACT 1 cut(s) 599
Bme18I GGWCC 1 cut(s) 441
BmeRI GACNNNNNGTC 1 cut(s) 459
BmgT120I GGNCC 1 cut(s) 441
BmiI GGNNCC 3 cut(s) 204, 442, 625
BmsI GCATC 2 cut(s) 251, 406
BoxI GACNNNNGTC 1 cut(s) 356
BpiI GAAGAC 2 cut(s) 312, 458
Bsa29I ATCGAT 1 cut(s) 647
BsaJI CCNNGG 1 cut(s) 444
BsaWI WCCGGW 1 cut(s) 626
BseAI TCCGGA 1 cut(s) 626
BseCI ATCGAT 1 cut(s) 647
BseDI CCNNGG 1 cut(s) 444
BseGI GGATG 4 cut(s) 160, 166, 242, 421
BseMII CTCAG 3 cut(s) 362, 474, 615
BseRI GAGGAG 1 cut(s) 215
BseXI GCAGC 1 cut(s) 331
BshVI ATCGAT 1 cut(s) 647
BsiHKAI GWGCWC 1 cut(s) 529
BsiSI CCGG 1 cut(s) 627
BslFI GGGAC 1 cut(s) 454
BsmAI GTCTC 1 cut(s) 68
BsmFI GGGAC 1 cut(s) 454
Bsp1286I GDGCHC 1 cut(s) 529
Bsp13I TCCGGA 1 cut(s) 626
Bsp143I GATC 2 cut(s) 116, 644
Bsp19I CCATGG 1 cut(s) 444
BspACI CCGC 1 cut(s) 545
BspCNI CTCAG 3 cut(s) 361, 475, 614
BspDI ATCGAT 1 cut(s) 647
BspEI TCCGGA 1 cut(s) 626
BspLI GGNNCC 3 cut(s) 204, 442, 625
BspPI GGATC 2 cut(s) 124, 652
BssECI CCNNGG 1 cut(s) 444
BssMI GATC 2 cut(s) 116, 644
BssT1I CCWWGG 1 cut(s) 444
BstDEI CTNAG 4 cut(s) 348, 483, 594, 601
BstDSI CCRYGG 1 cut(s) 444
BstF5I GGATG 4 cut(s) 160, 166, 242, 421
BstKTI GATC 2 cut(s) 119, 647
BstMAI GTCTC 1 cut(s) 68
BstMBI GATC 2 cut(s) 116, 644
BstMWI GCNNNNNNNGC 2 cut(s) 211, 353
BstPAI GACNNNNGTC 1 cut(s) 356
BstSFI CTRYAG 1 cut(s) 639
BstV1I GCAGC 1 cut(s) 331
BstV2I GAAGAC 2 cut(s) 312, 458
Bsu15I ATCGAT 1 cut(s) 647
BsuI GTATCC 1 cut(s) 99
BsuTUI ATCGAT 1 cut(s) 647
BtgI CCRYGG 1 cut(s) 444
BtsCI GGATG 4 cut(s) 160, 166, 242, 421
CaiI CAGNNNCTG 1 cut(s) 113
Cfr13I GGNCC 1 cut(s) 441
ClaI ATCGAT 1 cut(s) 647
CseI GACGC 1 cut(s) 317
Csp6I GTAC 1 cut(s) 598
CviAII CATG 2 cut(s) 392, 445
CviJI RGCY 5 cut(s) 138, 205, 298, 527, 605
CviKI_1 RGCY 5 cut(s) 138, 205, 298, 527, 605
CviQI GTAC 1 cut(s) 598
DdeI CTNAG 4 cut(s) 348, 483, 594, 601
DpnI GATC 2 cut(s) 118, 646
DpnII GATC 2 cut(s) 116, 644
DrdI GACNNNNNNGTC 1 cut(s) 82
DriI GACNNNNNGTC 1 cut(s) 459
DseDI GACNNNNNNGTC 1 cut(s) 82
Eam1105I GACNNNNNGTC 1 cut(s) 459
Ecl136II GAGCTC 1 cut(s) 527
Eco130I CCWWGG 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 529
Eco47I GGWCC 1 cut(s) 441
Eco53kI GAGCTC 1 cut(s) 527
EcoICRI GAGCTC 1 cut(s) 527
EcoT14I CCWWGG 1 cut(s) 444
EcoT38I GRGCYC 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 2 cut(s) 395, 448
FaiI YATR 7 cut(s) 393, 436, 438, 446, 585, 636, 652
FaqI GGGAC 1 cut(s) 454
FatI CATG 2 cut(s) 391, 444
Fnu4HI GCNGC 1 cut(s) 345
FokI GGATG 4 cut(s) 153, 167, 229, 428
FriOI GRGCYC 1 cut(s) 529
Fsp4HI GCNGC 1 cut(s) 345
GluI GCNGC 1 cut(s) 345
HapII CCGG 1 cut(s) 627
HgaI GACGC 1 cut(s) 317
Hin1II CATG 2 cut(s) 395, 448
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 6 cut(s) 95, 110, 125, 175, 487, 548
HpaII CCGG 1 cut(s) 627
HphI GGTGA 4 cut(s) 3, 59, 314, 410
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 5 cut(s) 109, 124, 351, 484, 538
Hpy188III TCNNGA 9 cut(s) 92, 99, 114, 131, 170, 179, 522, 573, 627
Hpy8I GTNNAC 1 cut(s) 25
HpyAV CCTTC 2 cut(s) 44, 618
HpyCH4V TGCA 1 cut(s) 287
HpyF10VI GCNNNNNNNGC 2 cut(s) 211, 353
HpyF3I CTNAG 4 cut(s) 348, 483, 594, 601
Hsp92II CATG 2 cut(s) 395, 448
Kpn2I TCCGGA 1 cut(s) 626
Kzo9I GATC 2 cut(s) 116, 644
LmnI GCTCC 4 cut(s) 202, 316, 524, 532
Lsp1109I GCAGC 1 cut(s) 331
LweI GCATC 2 cut(s) 251, 406
MalI GATC 2 cut(s) 118, 646
MboI GATC 2 cut(s) 116, 644
MboII GAAGA 5 cut(s) 50, 317, 413, 463, 599
MfeI CAATTG 2 cut(s) 288, 315
MhlI GDGCHC 1 cut(s) 529
MluCI AATT 7 cut(s) 259, 288, 315, 431, 504, 531, 567
MlyI GAGTC 1 cut(s) 542
MnlI CCTC 3 cut(s) 193, 223, 406
MroI TCCGGA 1 cut(s) 626
MroXI GAANNNNTTC 1 cut(s) 622
MseI TTAA 2 cut(s) 225, 558
MslI CAYNNNNRTG 1 cut(s) 396
MspI CCGG 1 cut(s) 627
MunI CAATTG 2 cut(s) 288, 315
MwoI GCNNNNNNNGC 2 cut(s) 211, 353
NcoI CCATGG 1 cut(s) 444
NdeII GATC 2 cut(s) 116, 644
NlaIII CATG 2 cut(s) 395, 448
NlaIV GGNNCC 3 cut(s) 204, 442, 625
PdmI GAANNNNTTC 1 cut(s) 622
PfeI GAWTC 5 cut(s) 95, 110, 125, 175, 487
PkrI GCNGC 1 cut(s) 346
PleI GAGTC 1 cut(s) 542
PpsI GAGTC 1 cut(s) 542
PshAI GACNNNNGTC 1 cut(s) 356
Psp124BI GAGCTC 1 cut(s) 529
PspN4I GGNNCC 3 cut(s) 204, 442, 625
PspPI GGNCC 1 cut(s) 441
PstNI CAGNNNCTG 1 cut(s) 113
RsaI GTAC 1 cut(s) 599
RsaNI GTAC 1 cut(s) 598
RseI CAYNNNNRTG 1 cut(s) 396
SacI GAGCTC 1 cut(s) 529
SaqAI TTAA 2 cut(s) 225, 558
SatI GCNGC 1 cut(s) 345
Sau3AI GATC 2 cut(s) 116, 644
Sau96I GGNCC 1 cut(s) 441
ScaI AGTACT 1 cut(s) 599
SchI GAGTC 1 cut(s) 542
SduI GDGCHC 1 cut(s) 529
SetI ASST 5 cut(s) 140, 300, 515, 529, 625
SfaNI GCATC 2 cut(s) 251, 406
SfcI CTRYAG 1 cut(s) 639
SinI GGWCC 1 cut(s) 441
SmiMI CAYNNNNRTG 1 cut(s) 396
Sse9I AATT 7 cut(s) 259, 288, 315, 431, 504, 531, 567
SsiI CCGC 1 cut(s) 545
SstI GAGCTC 1 cut(s) 529
StyI CCWWGG 1 cut(s) 444
TaqI TCGA 2 cut(s) 100, 647
TasI AATT 7 cut(s) 259, 288, 315, 431, 504, 531, 567
TatI WGTACW 1 cut(s) 597
TfiI GAWTC 5 cut(s) 95, 110, 125, 175, 487
Tru1I TTAA 2 cut(s) 225, 558
Tru9I TTAA 2 cut(s) 225, 558
TseI GCWGC 1 cut(s) 344
TspDTI ATGAA 3 cut(s) 272, 441, 600
TspGWI ACGGA 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 441
XapI RAATTY 2 cut(s) 259, 567
XmnI GAANNNNTTC 1 cut(s) 622
ZrmI AGTACT 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.