Rh2CG277100

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0007149
Physical Location & Seq
Forward (+)
30515770 .. 30521404
5635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG277100.1

Sequence Viewer

Length: 2100 bp
ATGACCACTGACTCGAACAGGAAGAGCAGCTCCGATCATGAATACCAACATGCAGTTGTGCCCAAAGAAGAGATCTTGGAGTTTGAGTTGCCTTCACCTCATGCATTCCCAACCCATTTCGGGAACAATGATGCAAGTTCCTCGGGGAGCAATATGAGAGCAAATATGATTGGAATGGGATTTTCGCCGTCCCGCGTCGACAAAGTGATTGAAGAAAAGGGTGAAGAGGACGCCGAACTGTTGTTGGAGACACTTTTATCATATCAAGATACGCAGAAATCAGATTCTGGGTCATCAGATTCTCTGGATAGTTTATTTGGCGACAAAGATGCAAGCAGTGCTCTCGAGGTTTCTAGTATGATTCAACCAAAAGAGGAACCTGATTTATCTGATGGACTTGATGATGGAAAAAAGGCGTCCTTACTTATGATGAATTTTTCTAGAGATGAAGTTCAGTTTGCAATGAATAGGCTTGGTGAAGATGCTCCAATCAATGACTTGGTGGATTTTATCATTGCTTCTCAGATATCTGTGAAACTTGAGAATGATGTAGATTCAACCCCGGTTGATGAAGAAAAGGAGGATACAAGTGATGAAAAATTATATGGAATCATGGAGAAGTCAGTTCGTTTGCTTGAAATGGGGTTCTCTGAGAACCAAGTTTCTTTTGCAATTGAAAAGTTTGGCTCTGGAGCTCCACTTACAGATCTTGCAGAATCAATAGTTACAGGTCAAATTTATGATAATTGCTATGACAAAAAAATTAAGGTAGCATCCCATTCAAATCATTCACGCAATGTGTCCAGTTTTTTCGGAACGGCATCATATGGTTCAGTAAAAGTTGAAAATGAGGAATTTTATCCAGATACAGCTTCTCGATCAAGGGATCGTGACTTGGCAGATAATTATTTAGGGAAGCGGCCTAAGCAAGAGATCATTGATGATTCAAATTCTGTTCCTCAGTTTAGGGTGTCCAGGCCTATAGATTTCAGGGAAAATCGTAAAGGGAAAAGGCCAAAACGAGAGTATATTGATGATTCAAGCTCTTCTGCATGGATGGAAGAAAAAGTAGACCCTGAGATTTGCAAAGTTGGGAATCCAAAACTTCAGAGTGTGAACCAAATGGCCGCTAAACCCCCATATTTCTTCTATGGAAATGTTCTGAATCTACCATATGATTCCTGGTCCAAAGTTTCTCAATTCTTGTACGGCCATCAACCTGAATATGTCAATAATCAGTTCTTCTCAGCCTTGAGTAGAAGGGAAGGGTATGTACACAATCTCCCAACTGAGAACAGGTTTCGTATCCTTGAAGAGCCACCAATGACCATTCAGGATGCAATACCATATGCAAAGAATTTGTGGCCTCCGTGGGATACAAGGAAGCAATTGACCTGCATCAGTTCTGAAACCAGTGGCATATCTCAGCTGTGTGACAGGCTTGGAAGAATGGTTTCTGATTGTCGTGGAATGCTCTCACCTGAGAAACAGAGGGAAATCCTTCATCATTGCAACTCATTAAATCTTGTATGGGTTGGCCGAAACAAGTTGGGTCCTTTACAGCCTGAATACTTGGAACAAATCTTAGGCTATCCACTGAATCACACTAAAGTTGGTGAGAGTGGTCTAATTGAAAGACTTACTTCCCTCAAGTATTGCTTCCAGACAGACACATTGGCTTATCATCTTTCTGTTTTAAAGGCTTTGTACCCAGAAGGATTAACAATGTTCTCAATTTTTAGTGGAATAGGTGGAGCAGAGATAGCTTTACACAAGCTTGGCATTCCTTTGAAAGGTGTTGTATCTGTAGAGACTTGTGGAACAAAGAGAAGGATTCTTCGGAGGTGGTGGGAAACTACTGGCCAAATTGGGCAGTTGGAGCAAATTGATGACATCCAGAAACTAACAAGCAGCAAGCTCGAAAGTCTGATGAAAAGGTTTGGTGGTTTTGATTTCCTTATTTGTCAGAATCCATGTAGTTCTTCAGTTTCTAAAATTCCTGCGCAAAGTGACAGTGATCCAGGCTTTGACTTCTCATTGTTTTATGAGTTTGTTCGTGTCTATCAACGTGTAAGAAATATGATGGATAGAAAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

699

Amino Acids

78.94

Weight (kDa)

5.15

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2004
Acc36I ACCTGC 1 cut(s) 1403
AccI GTMKAC 2 cut(s) 198, 1071
AccII CGCG 1 cut(s) 195
AciI CCGC 3 cut(s) 193, 919, 1128
AclWI GGATC 2 cut(s) 894, 2012
AcoI YGGCCR 4 cut(s) 1125, 1210, 1537, 1861
AcsI RAATTY 6 cut(s) 433, 735, 854, 949, 1357, 1995
AcuI CTGAAG 2 cut(s) 1091, 1968
AcyI GRCGYC 2 cut(s) 231, 416
AfaI GTAC 3 cut(s) 1208, 1275, 1709
AfiI CCNNNNNNNGG 2 cut(s) 120, 1793
AflIII ACRYGT 1 cut(s) 2068
AjnI CCWGG 3 cut(s) 974, 1181, 2020
AluBI AGCT 9 cut(s) 30, 695, 872, 1044, 1429, 1766, 1777, 1918, 2097
AluI AGCT 9 cut(s) 30, 695, 872, 1044, 1429, 1766, 1777, 1918, 2097
Alw21I GWGCWC 2 cut(s) 343, 697
Alw26I GTCTC 2 cut(s) 242, 1805
AlwI GGATC 2 cut(s) 894, 2012
AlwNI CAGNNNCTG 1 cut(s) 287
Ama87I CYCGRG 2 cut(s) 142, 344
AoxI GGCC 8 cut(s) 920, 977, 1013, 1125, 1210, 1364, 1537, 1861
ApeKI GCWGC 2 cut(s) 27, 1911
ApoI RAATTY 6 cut(s) 433, 735, 854, 949, 1357, 1995
Asp700I GAANNNNTTC 2 cut(s) 1453, 1500
AspLEI GCGC 1 cut(s) 2005
AspS9I GGNCC 2 cut(s) 1185, 1553
AsuC2I CCSGG 1 cut(s) 563
AsuHPI GGTGA 5 cut(s) 87, 233, 488, 1470, 1628
AvaI CYCGRG 2 cut(s) 142, 344
AvaII GGWCC 2 cut(s) 1185, 1553
BaeGI GKGCMC 1 cut(s) 63
BalI TGGCCA 1 cut(s) 1863
BanII GRGCYC 1 cut(s) 697
BarI GAAGNNNNNNTAC 2 cut(s) 1257, 1289
Bbv12I GWGCWC 2 cut(s) 343, 697
BbvI GCAGC 2 cut(s) 39, 1923
BccI CCATC 5 cut(s) 386, 398, 1051, 1221, 2077
BceAI ACGGC 3 cut(s) 172, 834, 1225
BcgI CGANNNNNNTGC 8 cut(s) 123, 157, 311, 325, 345, 359, 1900, 1934
BciT130I CCWGG 3 cut(s) 976, 1183, 2022
BciVI GTATCC 3 cut(s) 577, 1316, 1369
BcnI CCSGG 1 cut(s) 563
BcoDI GTCTC 2 cut(s) 242, 1805
BfaI CTAG 2 cut(s) 354, 441
BfmI CTRYAG 2 cut(s) 981, 1806
BfuAI ACCTGC 1 cut(s) 1403
BfuI GTATCC 3 cut(s) 577, 1316, 1369
BglII AGATCT 2 cut(s) 72, 706
BisI GCNGC 4 cut(s) 28, 920, 1128, 1912
BlsI GCNGC 4 cut(s) 29, 921, 1129, 1913
Bme1390I CCNGG 4 cut(s) 563, 976, 1183, 2022
Bme18I GGWCC 2 cut(s) 1185, 1553
BmeT110I CYCGRG 2 cut(s) 142, 344
BmgT120I GGNCC 2 cut(s) 1185, 1553
BmiI GGNNCC 2 cut(s) 378, 1554
BmrFI CCNGG 4 cut(s) 563, 976, 1183, 2022
BmsI GCATC 7 cut(s) 121, 319, 472, 782, 830, 1327, 1407
BpmI CTGGAG 1 cut(s) 711
Bpu10I CCTNAGC 1 cut(s) 924
BpuEI CTTGAG 3 cut(s) 560, 1273, 1634
BpuMI CCSGG 1 cut(s) 563
BsaHI GRCGYC 2 cut(s) 231, 416
BsaJI CCNNGG 3 cut(s) 141, 561, 1370
BsaXI ACNNNNNCTCC 4 cut(s) 71, 101, 1266, 1296
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 1793
Bse1I ACTGG 3 cut(s) 804, 1413, 1864
Bse3DI GCAATG 4 cut(s) 468, 513, 802, 1507
BseBI CCWGG 3 cut(s) 976, 1183, 2022
BseDI CCNNGG 3 cut(s) 141, 561, 1370
BseGI GGATG 4 cut(s) 773, 1062, 1342, 1893
BseLI CCNNNNNNNGG 2 cut(s) 120, 1793
BseMI GCAATG 4 cut(s) 468, 513, 802, 1507
BseMII CTCAG 8 cut(s) 536, 642, 974, 1068, 1260, 1281, 1439, 1473
BseNI ACTGG 3 cut(s) 804, 1413, 1864
BseSI GKGCMC 1 cut(s) 63
BseXI GCAGC 2 cut(s) 39, 1923
Bsh1236I CGCG 1 cut(s) 195
BshFI GGCC 8 cut(s) 922, 979, 1015, 1127, 1212, 1366, 1539, 1863
BsiHKAI GWGCWC 2 cut(s) 343, 697
BsiHKCI CYCGRG 2 cut(s) 142, 344
BsiSI CCGG 1 cut(s) 563
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 2 cut(s) 120, 1793
BsmAI GTCTC 2 cut(s) 242, 1805
BsmFI GGGAC 1 cut(s) 175
BsmI GAATGC 3 cut(s) 104, 1476, 1782
BsnI GGCC 8 cut(s) 922, 979, 1015, 1127, 1212, 1366, 1539, 1863
BsoBI CYCGRG 2 cut(s) 142, 344
Bsp1286I GDGCHC 3 cut(s) 63, 343, 697
Bsp1407I TGTACA 1 cut(s) 1273
Bsp143I GATC 7 cut(s) 34, 72, 706, 878, 886, 933, 2017
BspACI CCGC 3 cut(s) 193, 919, 1128
BspANI GGCC 8 cut(s) 922, 979, 1015, 1127, 1212, 1366, 1539, 1863
BspCNI CTCAG 8 cut(s) 535, 643, 973, 1069, 1259, 1282, 1438, 1474
BspFNI CGCG 1 cut(s) 195
BspHI TCATGA 1 cut(s) 37
BspLI GGNNCC 2 cut(s) 378, 1554
BspMI ACCTGC 1 cut(s) 1403
BspPI GGATC 2 cut(s) 894, 2012
BspQI GCTCTTC 3 cut(s) 17, 1051, 1308
BsrDI GCAATG 4 cut(s) 468, 513, 802, 1507
BsrGI TGTACA 1 cut(s) 1273
BsrI ACTGG 3 cut(s) 804, 1413, 1864
BssECI CCNNGG 3 cut(s) 141, 561, 1370
BssMI GATC 7 cut(s) 34, 72, 706, 878, 886, 933, 2017
BssNI GRCGYC 2 cut(s) 231, 416
Bst2UI CCWGG 3 cut(s) 976, 1183, 2022
Bst4CI ACNGT 2 cut(s) 240, 2015
Bst6I CTCTTC 5 cut(s) 17, 63, 219, 1051, 1308
BstACI GRCGYC 2 cut(s) 231, 416
BstAPI GCANNNNNTGC 1 cut(s) 338
BstAUI TGTACA 1 cut(s) 1273
BstC8I GCNNGC 2 cut(s) 334, 1916
BstDSI CCRYGG 1 cut(s) 1370
BstENI CCTNNNNNAGG 1 cut(s) 1791
BstF5I GGATG 4 cut(s) 773, 1062, 1342, 1893
BstFNI CGCG 1 cut(s) 195
BstHHI GCGC 1 cut(s) 2005
BstKTI GATC 7 cut(s) 37, 75, 709, 881, 889, 936, 2020
BstMAI GTCTC 2 cut(s) 242, 1805
BstMBI GATC 7 cut(s) 34, 72, 706, 878, 886, 933, 2017
BstMWI GCNNNNNNNGC 4 cut(s) 338, 925, 1763, 1879
BstNI CCWGG 3 cut(s) 976, 1183, 2022
BstNSI RCATGY 1 cut(s) 53
BstSCI CCNGG 4 cut(s) 561, 974, 1181, 2020
BstSFI CTRYAG 2 cut(s) 981, 1806
BstSLI GKGCMC 1 cut(s) 63
BstUI CGCG 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 39, 1923
BstX2I RGATCY 2 cut(s) 72, 706
BstYI RGATCY 2 cut(s) 72, 706
BsuI GTATCC 3 cut(s) 577, 1316, 1369
BsuRI GGCC 8 cut(s) 922, 979, 1015, 1127, 1212, 1366, 1539, 1863
BtgI CCRYGG 1 cut(s) 1370
BtsCI GGATG 4 cut(s) 773, 1062, 1342, 1893
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 5 cut(s) 6, 343, 1420, 1595, 2020
BveI ACCTGC 1 cut(s) 1403
Cac8I GCNNGC 2 cut(s) 334, 1916
CaiI CAGNNNCTG 1 cut(s) 287
CciI TCATGA 1 cut(s) 37
CfoI GCGC 1 cut(s) 2005
Cfr13I GGNCC 2 cut(s) 1185, 1553
CseI GACGC 3 cut(s) 184, 239, 405
Csp6I GTAC 3 cut(s) 1207, 1274, 1708
CviAII CATG 6 cut(s) 38, 50, 101, 613, 1053, 1974
CviQI GTAC 3 cut(s) 1207, 1274, 1708
DpnI GATC 7 cut(s) 36, 74, 708, 880, 888, 935, 2019
DpnII GATC 7 cut(s) 34, 72, 706, 878, 886, 933, 2017
DraI TTTAAA 1 cut(s) 1698
EaeI YGGCCR 4 cut(s) 1125, 1210, 1537, 1861
Eam1104I CTCTTC 5 cut(s) 17, 63, 219, 1051, 1308
EarI CTCTTC 5 cut(s) 17, 63, 219, 1051, 1308
Ecl136II GAGCTC 1 cut(s) 695
Eco147I AGGCCT 1 cut(s) 979
Eco24I GRGCYC 1 cut(s) 697
Eco32I GATATC 1 cut(s) 528
Eco47I GGWCC 2 cut(s) 1185, 1553
Eco53kI GAGCTC 1 cut(s) 695
Eco57I CTGAAG 2 cut(s) 1091, 1968
Eco88I CYCGRG 2 cut(s) 142, 344
EcoICRI GAGCTC 1 cut(s) 695
EcoNI CCTNNNNNAGG 1 cut(s) 1791
EcoO109I RGGNCCY 1 cut(s) 1553
EcoRII CCWGG 3 cut(s) 974, 1181, 2020
EcoRV GATATC 1 cut(s) 528
EcoT22I ATGCAT 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 697
FaeI CATG 6 cut(s) 41, 53, 104, 616, 1056, 1977
FalI AAGNNNNNCTT 6 cut(s) 404, 436, 1627, 1659, 1643, 1675
FaqI GGGAC 1 cut(s) 175
FatI CATG 6 cut(s) 37, 49, 100, 612, 1052, 1973
FauI CCCGC 1 cut(s) 200
FauNDI CATATG 3 cut(s) 826, 1174, 1348
FblI GTMKAC 2 cut(s) 198, 1071
Fnu4HI GCNGC 4 cut(s) 28, 920, 1128, 1912
FokI GGATG 4 cut(s) 760, 1069, 1349, 1880
FriOI GRGCYC 1 cut(s) 697
Fsp4HI GCNGC 4 cut(s) 28, 920, 1128, 1912
FspBI CTAG 2 cut(s) 354, 441
FspI TGCGCA 1 cut(s) 2004
GlaI GCGC 1 cut(s) 2004
GluI GCNGC 4 cut(s) 28, 920, 1128, 1912
GsuI CTGGAG 1 cut(s) 711
HaeIII GGCC 8 cut(s) 922, 979, 1015, 1127, 1212, 1366, 1539, 1863
HapII CCGG 1 cut(s) 563
HgaI GACGC 3 cut(s) 184, 239, 405
HhaI GCGC 1 cut(s) 2005
Hin1I GRCGYC 2 cut(s) 231, 416
Hin1II CATG 6 cut(s) 41, 53, 104, 616, 1056, 1977
Hin6I GCGC 1 cut(s) 2003
HinP1I GCGC 1 cut(s) 2003
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HindIII AAGCTT 1 cut(s) 1775
HpaII CCGG 1 cut(s) 563
HphI GGTGA 5 cut(s) 87, 233, 488, 1470, 1628
Hpy166II GTNNAC 4 cut(s) 199, 1072, 1117, 1276
Hpy8I GTNNAC 4 cut(s) 199, 1072, 1117, 1276
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 6 cut(s) 102, 1254, 1259, 1511, 1709, 1824
HpyCH4III ACNGT 2 cut(s) 240, 2015
HpyCH4IV ACGT 1 cut(s) 2068
HpyF10VI GCNNNNNNNGC 4 cut(s) 338, 925, 1763, 1879
HpySE526I ACGT 1 cut(s) 2068
Hsp92I GRCGYC 2 cut(s) 231, 416
Hsp92II CATG 6 cut(s) 41, 53, 104, 616, 1056, 1977
HspAI GCGC 1 cut(s) 2003
Kzo9I GATC 7 cut(s) 34, 72, 706, 878, 886, 933, 2017
LguI GCTCTTC 3 cut(s) 17, 1051, 1308
LmnI GCTCC 7 cut(s) 35, 147, 490, 692, 700, 1754, 1879
Lsp1109I GCAGC 2 cut(s) 39, 1923
LweI GCATC 7 cut(s) 121, 319, 472, 782, 830, 1327, 1407
MaeI CTAG 2 cut(s) 354, 441
MaeII ACGT 1 cut(s) 2068
MaeIII GTNAC 4 cut(s) 724, 890, 1433, 2009
MalI GATC 7 cut(s) 36, 74, 708, 880, 888, 935, 2019
MboI GATC 7 cut(s) 34, 72, 706, 878, 886, 933, 2017
MfeI CAATTG 2 cut(s) 672, 1388
MflI RGATCY 2 cut(s) 72, 706
MhlI GDGCHC 3 cut(s) 63, 343, 697
MlsI TGGCCA 1 cut(s) 1863
MluNI TGGCCA 1 cut(s) 1863
MlyI GAGTC 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 225, 1857
Mox20I TGGCCA 1 cut(s) 1863
Mph1103I ATGCAT 1 cut(s) 106
MroXI GAANNNNTTC 2 cut(s) 1453, 1500
MscI TGGCCA 1 cut(s) 1863
MseI TTAA 4 cut(s) 765, 1520, 1697, 1721
Msp20I TGGCCA 1 cut(s) 1863
MspA1I CMGCKG 1 cut(s) 1429
MspI CCGG 1 cut(s) 563
MspR9I CCNGG 4 cut(s) 563, 976, 1183, 2022
MunI CAATTG 2 cut(s) 672, 1388
Mva1269I GAATGC 3 cut(s) 104, 1476, 1782
MvaI CCWGG 3 cut(s) 976, 1183, 2022
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 4 cut(s) 338, 925, 1763, 1879
NciI CCSGG 1 cut(s) 563
NdeI CATATG 3 cut(s) 826, 1174, 1348
NdeII GATC 7 cut(s) 34, 72, 706, 878, 886, 933, 2017
NlaIII CATG 6 cut(s) 41, 53, 104, 616, 1056, 1977
NlaIV GGNNCC 2 cut(s) 378, 1554
NmuCI GTSAC 3 cut(s) 890, 1433, 2009
NsbI TGCGCA 1 cut(s) 2004
NsiI ATGCAT 1 cut(s) 106
NspI RCATGY 1 cut(s) 53
PaeR7I CTCGAG 1 cut(s) 344
PagI TCATGA 1 cut(s) 37
PceI AGGCCT 1 cut(s) 979
PciSI GCTCTTC 3 cut(s) 17, 1051, 1308
PctI GAATGC 3 cut(s) 104, 1476, 1782
PdmI GAANNNNTTC 2 cut(s) 1453, 1500
PkrI GCNGC 4 cut(s) 29, 921, 1129, 1913
PleI GAGTC 1 cut(s) 5
PpsI GAGTC 1 cut(s) 5
PpuMI RGGWCCY 1 cut(s) 1553
Psp124BI GAGCTC 1 cut(s) 697
Psp5II RGGWCCY 1 cut(s) 1553
Psp6I CCWGG 3 cut(s) 974, 1181, 2020
PspGI CCWGG 3 cut(s) 974, 1181, 2020
PspN4I GGNNCC 2 cut(s) 378, 1554
PspPI GGNCC 2 cut(s) 1185, 1553
PspPPI RGGWCCY 1 cut(s) 1553
PstNI CAGNNNCTG 1 cut(s) 287
PsuI RGATCY 2 cut(s) 72, 706
PvuII CAGCTG 1 cut(s) 1429
RsaI GTAC 3 cut(s) 1208, 1275, 1709
RsaNI GTAC 3 cut(s) 1207, 1274, 1708
SacI GAGCTC 1 cut(s) 697
SalI GTCGAC 1 cut(s) 197
SapI GCTCTTC 3 cut(s) 17, 1051, 1308
SaqAI TTAA 4 cut(s) 765, 1520, 1697, 1721
SatI GCNGC 4 cut(s) 28, 920, 1128, 1912
Sau3AI GATC 7 cut(s) 34, 72, 706, 878, 886, 933, 2017
Sau96I GGNCC 2 cut(s) 1185, 1553
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 4 cut(s) 563, 976, 1183, 2022
SduI GDGCHC 3 cut(s) 63, 343, 697
SfaNI GCATC 7 cut(s) 121, 319, 472, 782, 830, 1327, 1407
SfcI CTRYAG 2 cut(s) 981, 1806
Sfr274I CTCGAG 1 cut(s) 344
SinI GGWCC 2 cut(s) 1185, 1553
SlaI CTCGAG 1 cut(s) 344
SmlI CTYRAG 4 cut(s) 344, 539, 1252, 1649
SmoI CTYRAG 4 cut(s) 344, 539, 1252, 1649
SseBI AGGCCT 1 cut(s) 979
SsiI CCGC 3 cut(s) 193, 919, 1128
SspMI CTAG 2 cut(s) 354, 441
SstI GAGCTC 1 cut(s) 697
StuI AGGCCT 1 cut(s) 979
StyD4I CCNGG 4 cut(s) 561, 974, 1181, 2020
TaaI ACNGT 2 cut(s) 240, 2015
TaiI ACGT 1 cut(s) 2071
TaqI TCGA 5 cut(s) 14, 198, 345, 877, 1920
TatI WGTACW 1 cut(s) 1273
TauI GCSGC 2 cut(s) 922, 1130
Tru1I TTAA 4 cut(s) 765, 1520, 1697, 1721
Tru9I TTAA 4 cut(s) 765, 1520, 1697, 1721
TscAI CASTG 5 cut(s) 13, 343, 1420, 1602, 2020
TseFI GTSAC 3 cut(s) 890, 1433, 2009
TseI GCWGC 2 cut(s) 27, 1911
Tsp45I GTSAC 3 cut(s) 890, 1433, 2009
TspDTI ATGAA 8 cut(s) 54, 446, 462, 479, 585, 609, 1493, 1946
TspGWI ACGGA 1 cut(s) 1359
TspRI CASTG 5 cut(s) 13, 343, 1420, 1602, 2020
VpaK11BI GGWCC 2 cut(s) 1185, 1553
XagI CCTNNNNNAGG 1 cut(s) 1791
XapI RAATTY 6 cut(s) 433, 735, 854, 949, 1357, 1995
XbaI TCTAGA 1 cut(s) 440
XceI RCATGY 1 cut(s) 53
XcmI CCANNNNNNNNNTGG 1 cut(s) 1179
XhoI CTCGAG 1 cut(s) 344
XmiI GTMKAC 2 cut(s) 198, 1071
XmnI GAANNNNTTC 2 cut(s) 1453, 1500
XspI CTAG 2 cut(s) 354, 441
Zsp2I ATGCAT 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.