pycom13g17440

Stem-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012461
Physical Location & Seq
Forward (+)
13199217 .. 13201391
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g17440.1

Sequence Viewer

Length: 753 bp
ATGCTGGCAGTGTTCAACAAGTCGGTCGCCAAAAGCCCCGAGGCGCTGCAGAGCCCTCAGTCCGGATCAGCCTCCGCCCTGAAAGATGGGTTTCTGGCGCGGCAGTTTGCCTCTGTGCACCCTTCCTCCGTCACCGTCAATCTTGGCTCTTCTGGTGTAATCGCCTACTCTCTCGACAAACAGAACCCTCTTCTTCCAAGGTTGTTTGCGGTTGTGGACGACATTTTCTGCCTGTTCCAAGGCCACATTGAGAACGTTGCTCTTCTTAAGCAACAATATGGATTGAACAAAACAGCCAATGAGGTGATCATTGTGATTGAAGCTTTCAGGACTCTGAGAGACCGGGGTCCCTATCCTGCTGACCAGGTTGTGAGAGATATCCAAGGGAAGTTTGCATTTGTTCTCTTTGACAGTGCTTCCAAAGCCGCTTTTGTAGCTGCTGATACTGATGGGAATGTTCCGTTCTTCTGGGGAACTGATTCTGAAGGCCATCTTGTGCTTGCAGATGATTCAGAAGTTGTGAAAAAGGGCTGCGGAAATTCTTTTGCACCATTTCCTAAAGGTTGTTTCTTCACTTCCTCTGGAGGCTTGAGGAGTTACGAGCATCCCCACAATGAGTTGAAGGCGGTGCCACGTGTGGATAGCTCAGGTGAAATGTGTGGTGCCAATTTCAAGGTCGATGCTGAAACTAAGAAGGAATCGAGTGGCATCCCAAGAGTCGGGAGTGCTGCCAACTGGTCGACAAATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3700

Protein Analysis

251

Amino Acids

26.87

Weight (kDa)

6.21

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3700 PF12481 2 - 226 1.1e-113 Aluminium induced protein
GATase_7 PF13537 74 - 168 2.3e-07 Glutamine amidotransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012461)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G22850 AT5G43830
fragaria_vesca FvH4_4g01560
malus_domestica MD13G1201000.v1.1 MD16G1200200.v1.1
prunus_persica Prupe.1G022400_v2.0.a1 Prupe.1G022400_v2.0.a1
pyrus_communis pycom13g17440 pycom16g16940
rosa_chinensis RchiOBHm_Chr4g0388131
rosa_laevigata RLG00000010078
rosa_multiflora Rmu_sc0002509.1_g000004
rosa_roxburghii Rroxscaffold_5G00334680
rosa_rugosa Rorug03G0315000
rosa_samantha Rh4AG020800 Rh4BG015200 Rh4CG021400 Rh4DG016000
rosa_wichuraiana Rw4G001390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 628, 662
AccI GTMKAC 1 cut(s) 740
AccII CGCG 1 cut(s) 100
AccIII TCCGGA 1 cut(s) 62
AciI CCGC 6 cut(s) 75, 100, 209, 426, 534, 626
AclI AACGTT 1 cut(s) 255
AclWI GGATC 1 cut(s) 73
AcsI RAATTY 1 cut(s) 538
AcuI CTGAAG 1 cut(s) 504
AcvI CACGTG 1 cut(s) 635
AfiI CCNNNNNNNGG 2 cut(s) 62, 719
AflII CTTAAG 1 cut(s) 266
AflIII ACRYGT 1 cut(s) 634
AgsI TTSAA 5 cut(s) 16, 286, 320, 622, 673
AjnI CCWGG 1 cut(s) 363
AluBI AGCT 3 cut(s) 323, 437, 645
AluI AGCT 3 cut(s) 323, 437, 645
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 1 cut(s) 333
Alw44I GTGCAC 1 cut(s) 116
AlwI GGATC 1 cut(s) 73
Ama87I CYCGRG 1 cut(s) 38
Aor13HI TCCGGA 1 cut(s) 62
AoxI GGCC 2 cut(s) 241, 487
ApaLI GTGCAC 1 cut(s) 116
ApeKI GCWGC 4 cut(s) 46, 437, 531, 728
ApoI RAATTY 1 cut(s) 538
Asp700I GAANNNNTTC 1 cut(s) 478
AspLEI GCGC 2 cut(s) 46, 100
AspS9I GGNCC 1 cut(s) 347
AsuC2I CCSGG 1 cut(s) 344
AsuHPI GGTGA 3 cut(s) 124, 316, 662
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 1 cut(s) 347
BaeGI GKGCMC 1 cut(s) 120
BanI GGYRCC 2 cut(s) 628, 662
BanII GRGCYC 1 cut(s) 56
BbrPI CACGTG 1 cut(s) 635
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 4 cut(s) 33, 424, 518, 715
BccI CCATC 3 cut(s) 80, 443, 498
BciT130I CCWGG 1 cut(s) 365
BclI TGATCA 1 cut(s) 306
BcnI CCSGG 1 cut(s) 344
BcoDI GTCTC 1 cut(s) 333
BfmI CTRYAG 1 cut(s) 47
BfoI RGCGCY 1 cut(s) 47
BfrI CTTAAG 1 cut(s) 266
BisI GCNGC 6 cut(s) 47, 101, 426, 438, 532, 729
BlsI GCNGC 6 cut(s) 48, 102, 427, 439, 533, 730
Bme1390I CCNGG 2 cut(s) 344, 365
Bme18I GGWCC 1 cut(s) 347
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 1 cut(s) 347
BmiI GGNNCC 4 cut(s) 348, 349, 630, 664
BmrFI CCNGG 2 cut(s) 344, 365
BmsI GCATC 3 cut(s) 613, 670, 717
BoxI GACNNNNGTC 1 cut(s) 345
BpmI CTGGAG 1 cut(s) 603
Bpu10I CCTNAGC 1 cut(s) 646
BpuEI CTTGAG 1 cut(s) 610
BpuMI CCSGG 1 cut(s) 344
BsaAI YACGTR 1 cut(s) 635
BsaI GGTCTC 1 cut(s) 333
BsaJI CCNNGG 5 cut(s) 39, 197, 238, 343, 382
BsaWI WCCGGW 1 cut(s) 62
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 719
Bse1I ACTGG 1 cut(s) 740
BseAI TCCGGA 1 cut(s) 62
BseBI CCWGG 1 cut(s) 365
BseDI CCNNGG 5 cut(s) 39, 197, 238, 343, 382
BseGI GGATG 2 cut(s) 604, 708
BseLI CCNNNNNNNGG 2 cut(s) 62, 719
BseMII CTCAG 3 cut(s) 71, 326, 660
BseNI ACTGG 1 cut(s) 740
BseRI GAGGAG 1 cut(s) 607
BseSI GKGCMC 1 cut(s) 120
BseXI GCAGC 4 cut(s) 33, 424, 518, 715
Bsh1236I CGCG 1 cut(s) 100
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 2 cut(s) 243, 489
BshNI GGYRCC 2 cut(s) 628, 662
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 120
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 2 cut(s) 63, 343
BslFI GGGAC 1 cut(s) 333
BslI CCNNNNNNNGG 2 cut(s) 62, 719
BsmAI GTCTC 1 cut(s) 333
BsmFI GGGAC 1 cut(s) 333
BsnI GGCC 2 cut(s) 243, 489
Bso31I GGTCTC 1 cut(s) 333
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 2 cut(s) 56, 120
Bsp13I TCCGGA 1 cut(s) 62
Bsp143I GATC 2 cut(s) 65, 306
BspACI CCGC 6 cut(s) 75, 100, 209, 426, 534, 626
BspANI GGCC 2 cut(s) 243, 489
BspCNI CTCAG 3 cut(s) 70, 327, 659
BspEI TCCGGA 1 cut(s) 62
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 4 cut(s) 348, 349, 630, 664
BspMAI CTGCAG 1 cut(s) 51
BspPI GGATC 1 cut(s) 73
BspQI GCTCTTC 2 cut(s) 154, 267
BspT107I GGYRCC 2 cut(s) 628, 662
BspTI CTTAAG 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 333
BsrI ACTGG 1 cut(s) 740
BssECI CCNNGG 5 cut(s) 39, 197, 238, 343, 382
BssMI GATC 2 cut(s) 65, 306
BssT1I CCWWGG 3 cut(s) 197, 238, 382
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 2 cut(s) 136, 413
Bst6I CTCTTC 3 cut(s) 154, 195, 267
BstAFI CTTAAG 1 cut(s) 266
BstBAI YACGTR 1 cut(s) 635
BstC8I GCNNGC 2 cut(s) 6, 501
BstDEI CTNAG 4 cut(s) 57, 335, 646, 690
BstF5I GGATG 2 cut(s) 604, 708
BstFNI CGCG 1 cut(s) 100
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 2 cut(s) 46, 100
BstKTI GATC 2 cut(s) 68, 309
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 2 cut(s) 65, 306
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 2 cut(s) 422, 434
BstNI CCWGG 1 cut(s) 365
BstPAI GACNNNNGTC 1 cut(s) 345
BstSCI CCNGG 2 cut(s) 342, 363
BstSFI CTRYAG 1 cut(s) 47
BstSLI GKGCMC 1 cut(s) 120
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 4 cut(s) 33, 424, 518, 715
BsuRI GGCC 2 cut(s) 243, 489
BtsCI GGATG 2 cut(s) 604, 708
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 2 cut(s) 15, 418
Cac8I GCNNGC 2 cut(s) 6, 501
CfoI GCGC 2 cut(s) 46, 100
Cfr13I GGNCC 1 cut(s) 347
CsiI ACCWGGT 1 cut(s) 363
DdeI CTNAG 4 cut(s) 57, 335, 646, 690
DpnI GATC 2 cut(s) 67, 308
DpnII GATC 2 cut(s) 65, 306
Eam1104I CTCTTC 3 cut(s) 154, 195, 267
EarI CTCTTC 3 cut(s) 154, 195, 267
EciI GGCGGA 1 cut(s) 64
Eco130I CCWWGG 3 cut(s) 197, 238, 382
Eco24I GRGCYC 1 cut(s) 56
Eco31I GGTCTC 1 cut(s) 333
Eco32I GATATC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 504
Eco72I CACGTG 1 cut(s) 635
Eco88I CYCGRG 1 cut(s) 38
EcoO109I RGGNCCY 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 363
EcoRV GATATC 1 cut(s) 379
EcoT14I CCWWGG 3 cut(s) 197, 238, 382
EcoT38I GRGCYC 1 cut(s) 56
ErhI CCWWGG 3 cut(s) 197, 238, 382
FaiI YATR 1 cut(s) 279
FalI AAGNNNNNCTT 2 cut(s) 477, 509
FaqI GGGAC 1 cut(s) 333
FbaI TGATCA 1 cut(s) 306
FblI GTMKAC 1 cut(s) 740
Fnu4HI GCNGC 6 cut(s) 47, 101, 426, 438, 532, 729
FokI GGATG 2 cut(s) 591, 695
FriOI GRGCYC 1 cut(s) 56
Fsp4HI GCNGC 6 cut(s) 47, 101, 426, 438, 532, 729
GlaI GCGC 2 cut(s) 45, 99
GluI GCNGC 6 cut(s) 47, 101, 426, 438, 532, 729
GsuI CTGGAG 1 cut(s) 603
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 2 cut(s) 243, 489
HapII CCGG 2 cut(s) 63, 343
HhaI GCGC 2 cut(s) 46, 100
Hin6I GCGC 2 cut(s) 44, 98
HinP1I GCGC 2 cut(s) 44, 98
HincII GTYRAC 1 cut(s) 741
HindII GTYRAC 1 cut(s) 741
HindIII AAGCTT 1 cut(s) 321
HinfI GANTC 5 cut(s) 331, 479, 509, 698, 717
HpaII CCGG 2 cut(s) 63, 343
HphI GGTGA 3 cut(s) 124, 316, 662
Hpy166II GTNNAC 3 cut(s) 118, 217, 741
Hpy188I TCNGA 3 cut(s) 336, 484, 514
Hpy188III TCNNGA 5 cut(s) 63, 173, 328, 582, 721
Hpy8I GTNNAC 3 cut(s) 118, 217, 741
HpyAV CCTTC 4 cut(s) 132, 479, 616, 688
HpyCH4III ACNGT 2 cut(s) 136, 413
HpyCH4IV ACGT 2 cut(s) 255, 634
HpyCH4V TGCA 5 cut(s) 49, 118, 395, 503, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 434
HpyF3I CTNAG 4 cut(s) 57, 335, 646, 690
HpySE526I ACGT 2 cut(s) 255, 634
HspAI GCGC 2 cut(s) 44, 98
KflI GGGWCCC 1 cut(s) 347
Kpn2I TCCGGA 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 306
Kzo9I GATC 2 cut(s) 65, 306
LguI GCTCTTC 2 cut(s) 154, 267
Lsp1109I GCAGC 4 cut(s) 33, 424, 518, 715
LweI GCATC 3 cut(s) 613, 670, 717
MabI ACCWGGT 1 cut(s) 363
MaeII ACGT 2 cut(s) 255, 634
MaeIII GTNAC 2 cut(s) 130, 596
MalI GATC 2 cut(s) 67, 308
MboI GATC 2 cut(s) 65, 306
MboII GAAGA 6 cut(s) 141, 182, 185, 254, 457, 562
MhlI GDGCHC 2 cut(s) 56, 120
MluCI AATT 3 cut(s) 538, 667, 745
MlyI GAGTC 2 cut(s) 325, 726
MroI TCCGGA 1 cut(s) 62
MroXI GAANNNNTTC 1 cut(s) 478
MseI TTAA 1 cut(s) 267
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 2 cut(s) 63, 343
MspR9I CCNGG 2 cut(s) 344, 365
MvaI CCWGG 1 cut(s) 365
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 2 cut(s) 422, 434
NciI CCSGG 1 cut(s) 344
NdeII GATC 2 cut(s) 65, 306
NlaIV GGNNCC 4 cut(s) 348, 349, 630, 664
NmuCI GTSAC 1 cut(s) 130
PciSI GCTCTTC 2 cut(s) 154, 267
PdmI GAANNNNTTC 1 cut(s) 478
PfeI GAWTC 3 cut(s) 479, 509, 698
PkrI GCNGC 6 cut(s) 48, 102, 427, 439, 533, 730
PleI GAGTC 2 cut(s) 325, 725
PmaCI CACGTG 1 cut(s) 635
PmlI CACGTG 1 cut(s) 635
PpsI GAGTC 2 cut(s) 325, 725
Ppu21I YACGTR 1 cut(s) 635
PpuMI RGGWCCY 1 cut(s) 347
PshAI GACNNNNGTC 1 cut(s) 345
Psp1406I AACGTT 1 cut(s) 255
Psp5II RGGWCCY 1 cut(s) 347
Psp6I CCWGG 1 cut(s) 363
PspCI CACGTG 1 cut(s) 635
PspGI CCWGG 1 cut(s) 363
PspN4I GGNNCC 4 cut(s) 348, 349, 630, 664
PspPI GGNCC 1 cut(s) 347
PspPPI RGGWCCY 1 cut(s) 347
PstI CTGCAG 1 cut(s) 51
SalI GTCGAC 1 cut(s) 739
SapI GCTCTTC 2 cut(s) 154, 267
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 6 cut(s) 47, 101, 426, 438, 532, 729
Sau3AI GATC 2 cut(s) 65, 306
Sau96I GGNCC 1 cut(s) 347
SchI GAGTC 2 cut(s) 325, 726
ScrFI CCNGG 2 cut(s) 344, 365
SduI GDGCHC 2 cut(s) 56, 120
SexAI ACCWGGT 1 cut(s) 363
SfaNI GCATC 3 cut(s) 613, 670, 717
SfcI CTRYAG 1 cut(s) 47
SinI GGWCC 1 cut(s) 347
SmlI CTYRAG 2 cut(s) 266, 589
SmoI CTYRAG 2 cut(s) 266, 589
Sse9I AATT 3 cut(s) 538, 667, 745
SsiI CCGC 6 cut(s) 75, 100, 209, 426, 534, 626
StyD4I CCNGG 2 cut(s) 342, 363
StyI CCWWGG 3 cut(s) 197, 238, 382
TaaI ACNGT 2 cut(s) 136, 413
TaiI ACGT 2 cut(s) 258, 637
TaqI TCGA 4 cut(s) 174, 678, 701, 740
TaqII GACCGA 1 cut(s) 13
TasI AATT 3 cut(s) 538, 667, 745
TauI GCSGC 2 cut(s) 103, 428
TfiI GAWTC 3 cut(s) 479, 509, 698
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 15, 418
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 4 cut(s) 46, 437, 531, 728
Tsp45I GTSAC 1 cut(s) 130
TspGWI ACGGA 2 cut(s) 118, 450
TspRI CASTG 2 cut(s) 15, 418
Vha464I CTTAAG 1 cut(s) 266
VneI GTGCAC 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 347
XapI RAATTY 1 cut(s) 538
XmiI GTMKAC 1 cut(s) 740
XmnI GAANNNNTTC 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.