pycom13g18470

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015265
Physical Location & Seq
Reverse (-)
14595553 .. 14596020
468 bp
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UTR
Exon/CDS
Intron
pycom13g18470.1

Sequence Viewer

Length: 468 bp
ATGGAAAACCCTCGCAGAGGAGAGGATCCAGAGAAGCAAGGAAAGCAAGAGAAGGGAAGCCAAGAAGTACGTTACCGCGGGGTCCGAAGAAGGCCGTGGGGGAAGTTTGCTTCTGAGATAAGAGACCCTTCTAAGCAAGGTGCTCGTCTGTGGCTTGGTACATTTGACACCGCTGAGGAAGCTGCAAGGGCTTATGATAATGCTGCCTTTAATTTAAGGGGTCATCTTGCCATACTAAATTTTCCAAATGAGTATTATGCTCAAGTTATGGGCTCTCCTCCTTATCCTCCTAGATTCTCGACACTTCCTTCTTCTTCTTCTTCTTCTTCCACTTCATCATCTTCTGCCGCTGCACCCGGTGGAAGTTCATCTGCTGGACAGGGAAGCAGGCAAGTGTTTGAGATTGAATACTTGGATAACAAGGTGTTAGAGGACCTTCTTGATTATGAAGAGGAGAAGAAAAAATGA

Protein Analysis

156

Amino Acids

17.24

Weight (kDa)

6.63

Isoelectric Point (pI)

75.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 24 - 75 3.4e-16 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23230
fragaria_vesca FvH4_4g03460
malus_domestica MD13G1212900.v1.1 MD16G1216500.v1.1
prunus_persica Prupe.1G037800_v2.0.a1
pyrus_communis pycom13g18470 pycom16g18110
rosa_chinensis RchiOBHm_Chr4g0392461
rosa_laevigata RLG00000009804
rosa_multiflora Rmu_sc0004216.1_g000004 Rmu_sc0041365.1_g000001
rosa_roxburghii Rroxscaffold_5G00337720
rosa_rugosa Rorug03G0339000
rosa_samantha Rh4BG038100 Rh4DG040100
rosa_wichuraiana Rw4G003370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 78
AciI CCGC 4 cut(s) 76, 78, 171, 348
AclWI GGATC 2 cut(s) 20, 33
AcsI RAATTY 1 cut(s) 238
AdeI CACNNNGTG 1 cut(s) 359
AfaI GTAC 2 cut(s) 69, 160
AfiI CCNNNNNNNGG 1 cut(s) 17
AgsI TTSAA 1 cut(s) 407
AluBI AGCT 1 cut(s) 182
AluI AGCT 1 cut(s) 182
Alw21I GWGCWC 1 cut(s) 145
Alw26I GTCTC 1 cut(s) 117
AlwI GGATC 2 cut(s) 20, 33
AoxI GGCC 1 cut(s) 92
ApeKI GCWGC 3 cut(s) 182, 203, 350
ApoI RAATTY 1 cut(s) 238
AspS9I GGNCC 2 cut(s) 82, 433
AsuC2I CCSGG 1 cut(s) 357
AvaII GGWCC 2 cut(s) 82, 433
BamHI GGATCC 1 cut(s) 25
BanII GRGCYC 1 cut(s) 275
Bbv12I GWGCWC 1 cut(s) 145
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 3 cut(s) 169, 190, 337
BceAI ACGGC 1 cut(s) 79
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BcnI CCSGG 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 1 cut(s) 291
BisI GCNGC 4 cut(s) 183, 204, 348, 351
BlsI GCNGC 4 cut(s) 184, 205, 349, 352
Bme1390I CCNGG 1 cut(s) 357
Bme18I GGWCC 2 cut(s) 82, 433
BmgT120I GGNCC 2 cut(s) 82, 433
BmiI GGNNCC 2 cut(s) 27, 83
BmrFI CCNGG 1 cut(s) 357
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 76, 95
Bsc4I CCNNNNNNNGG 1 cut(s) 17
BseDI CCNNGG 2 cut(s) 76, 95
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMII CTCAG 2 cut(s) 105, 165
BseRI GAGGAG 3 cut(s) 33, 267, 467
BseXI GCAGC 3 cut(s) 169, 190, 337
BsgI GTGCAG 1 cut(s) 336
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 145
BsiSI CCGG 1 cut(s) 357
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 117
BsnI GGCC 1 cut(s) 94
Bso31I GGTCTC 1 cut(s) 117
Bsp1286I GDGCHC 2 cut(s) 145, 275
Bsp143I GATC 1 cut(s) 25
BspACI CCGC 4 cut(s) 76, 78, 171, 348
BspANI GGCC 1 cut(s) 94
BspCNI CTCAG 2 cut(s) 106, 166
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 2 cut(s) 27, 83
BspPI GGATC 2 cut(s) 20, 33
BspTNI GGTCTC 1 cut(s) 117
BssECI CCNNGG 2 cut(s) 76, 95
BssMI GATC 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 444
BstC8I GCNNGC 1 cut(s) 389
BstDEI CTNAG 3 cut(s) 114, 132, 174
BstDSI CCRYGG 2 cut(s) 76, 95
BstENI CCTNNNNNAGG 1 cut(s) 15
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 1 cut(s) 28
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 1 cut(s) 25
BstMWI GCNNNNNNNGC 3 cut(s) 43, 179, 188
BstSCI CCNGG 1 cut(s) 355
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 3 cut(s) 169, 190, 337
BstX2I RGATCY 1 cut(s) 25
BstYI RGATCY 1 cut(s) 25
BsuRI GGCC 1 cut(s) 94
BtgI CCRYGG 2 cut(s) 76, 95
Cac8I GCNNGC 1 cut(s) 389
Cfr13I GGNCC 2 cut(s) 82, 433
Cfr42I CCGCGG 1 cut(s) 79
Csp6I GTAC 2 cut(s) 68, 159
CviJI RGCY 6 cut(s) 60, 94, 154, 182, 191, 273
CviKI_1 RGCY 6 cut(s) 60, 94, 154, 182, 191, 273
CviQI GTAC 2 cut(s) 68, 159
DdeI CTNAG 3 cut(s) 114, 132, 174
DpnI GATC 1 cut(s) 27
DpnII GATC 1 cut(s) 25
DraIII CACNNNGTG 1 cut(s) 359
Eam1104I CTCTTC 1 cut(s) 444
EarI CTCTTC 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 275
Eco31I GGTCTC 1 cut(s) 117
Eco47I GGWCC 2 cut(s) 82, 433
EcoNI CCTNNNNNAGG 1 cut(s) 15
EcoO109I RGGNCCY 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 275
FaiI YATR 5 cut(s) 195, 233, 258, 269, 447
FalI AAGNNNNNCTT 2 cut(s) 112, 144
FauI CCCGC 1 cut(s) 71
Fnu4HI GCNGC 4 cut(s) 183, 204, 348, 351
FriOI GRGCYC 1 cut(s) 275
Fsp4HI GCNGC 4 cut(s) 183, 204, 348, 351
FspBI CTAG 1 cut(s) 291
GluI GCNGC 4 cut(s) 183, 204, 348, 351
HaeIII GGCC 1 cut(s) 94
HapII CCGG 1 cut(s) 357
HinfI GANTC 1 cut(s) 294
HpaII CCGG 1 cut(s) 357
Hpy188I TCNGA 2 cut(s) 86, 115
Hpy188III TCNNGA 3 cut(s) 29, 298, 440
HpyAV CCTTC 5 cut(s) 46, 84, 138, 318, 446
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 2 cut(s) 185, 353
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 179, 188
HpyF3I CTNAG 3 cut(s) 114, 132, 174
HpySE526I ACGT 1 cut(s) 70
KspI CCGCGG 1 cut(s) 79
Kzo9I GATC 1 cut(s) 25
LpnPI CCDG 5 cut(s) 42, 360, 365, 370, 373
Lsp1109I GCAGC 3 cut(s) 169, 190, 337
MaeI CTAG 1 cut(s) 291
MaeII ACGT 1 cut(s) 70
MaeIII GTNAC 1 cut(s) 71
MalI GATC 1 cut(s) 27
MboI GATC 1 cut(s) 25
MboII GAAGA 9 cut(s) 99, 303, 306, 309, 312, 315, 318, 333, 461
MflI RGATCY 1 cut(s) 25
MhlI GDGCHC 2 cut(s) 145, 275
MluCI AATT 2 cut(s) 211, 238
MnlI CCTC 8 cut(s) 11, 16, 21, 169, 288, 297, 424, 445
MseI TTAA 2 cut(s) 210, 215
MspA1I CMGCKG 3 cut(s) 78, 173, 350
MspI CCGG 1 cut(s) 357
MspR9I CCNGG 1 cut(s) 357
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 3 cut(s) 43, 179, 188
NciI CCSGG 1 cut(s) 357
NdeII GATC 1 cut(s) 25
NlaIV GGNNCC 2 cut(s) 27, 83
PfeI GAWTC 1 cut(s) 294
PkrI GCNGC 4 cut(s) 184, 205, 349, 352
PpuMI RGGWCCY 1 cut(s) 433
Psp5II RGGWCCY 1 cut(s) 433
PspN4I GGNNCC 2 cut(s) 27, 83
PspPI GGNCC 2 cut(s) 82, 433
PspPPI RGGWCCY 1 cut(s) 433
PsuI RGATCY 1 cut(s) 25
RsaI GTAC 2 cut(s) 69, 160
RsaNI GTAC 2 cut(s) 68, 159
SacII CCGCGG 1 cut(s) 79
SaqAI TTAA 2 cut(s) 210, 215
SatI GCNGC 4 cut(s) 183, 204, 348, 351
Sau3AI GATC 1 cut(s) 25
Sau96I GGNCC 2 cut(s) 82, 433
ScrFI CCNGG 1 cut(s) 357
SduI GDGCHC 2 cut(s) 145, 275
SetI ASST 5 cut(s) 73, 142, 184, 426, 438
Sfr303I CCGCGG 1 cut(s) 79
SgrBI CCGCGG 1 cut(s) 79
SinI GGWCC 2 cut(s) 82, 433
SmlI CTYRAG 1 cut(s) 261
SmoI CTYRAG 1 cut(s) 261
Sse9I AATT 2 cut(s) 211, 238
SsiI CCGC 4 cut(s) 76, 78, 171, 348
SspMI CTAG 1 cut(s) 291
StyD4I CCNGG 1 cut(s) 355
TaiI ACGT 1 cut(s) 73
TaqI TCGA 1 cut(s) 299
TasI AATT 2 cut(s) 211, 238
TauI GCSGC 1 cut(s) 350
TfiI GAWTC 1 cut(s) 294
Tru1I TTAA 2 cut(s) 210, 215
Tru9I TTAA 2 cut(s) 210, 215
TseI GCWGC 3 cut(s) 182, 203, 350
TspDTI ATGAA 3 cut(s) 324, 357, 462
VpaK11BI GGWCC 2 cut(s) 82, 433
XagI CCTNNNNNAGG 1 cut(s) 15
XapI RAATTY 1 cut(s) 238
XspI CTAG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.