pycom16g18110

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0015265
Physical Location & Seq
Reverse (-)
14543601 .. 14544062
462 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g18110.1

Sequence Viewer

Length: 462 bp
ATGGAAAACCCTCGCAGAGGAAAGGATCAAGAGAAGCAAGGAAAGGAGGAGAAGGGAAGCGAAGAAGTACGTTACCGTGGGGTCCGAAGAAGGCCGTGGGGGAAGTTTGCTTCTGAGATAAGAGACCCTTCTAAGCAGGGTGCTCGTCTGTGGCTTGGTACATTTGACACCGCTGAGGAAGCTGCAAGGGCTTATGATAAGGCTGCCTTTAATTTAAGGGGTCATCTTGCCACACTAAATTTTCCAAATGAATATTATGCTCAAGTTATGGGCTCTCATCCTTATCCTCCTAGATTCTCGGCACTTTCTTCTTCTTCTTCGTCCACTTCATCATCTTCTGCTGCTGCACCGGGTGGAAGTTCATCTGCCGGACAGGGAAGTAGGCAAGTGTTTGAGATTGAGTACTTGGATAACAAGGTGTTGGAAGACCTCCTTGATTATGAAGAGGAGAAGAATAAATGA

Protein Analysis

154

Amino Acids

17.09

Weight (kDa)

6.76

Isoelectric Point (pI)

67.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 24 - 75 2.3e-16 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23230
fragaria_vesca FvH4_4g03460
malus_domestica MD13G1212900.v1.1 MD16G1216500.v1.1
prunus_persica Prupe.1G037800_v2.0.a1
pyrus_communis pycom13g18470 pycom16g18110
rosa_chinensis RchiOBHm_Chr4g0392461
rosa_laevigata RLG00000009804
rosa_multiflora Rmu_sc0004216.1_g000004 Rmu_sc0041365.1_g000001
rosa_roxburghii Rroxscaffold_5G00337720
rosa_rugosa Rorug03G0339000
rosa_samantha Rh4BG038100 Rh4DG040100
rosa_wichuraiana Rw4G003370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 171
AclWI GGATC 1 cut(s) 33
AcsI RAATTY 1 cut(s) 238
AdeI CACNNNGTG 1 cut(s) 353
AfaI GTAC 3 cut(s) 69, 160, 404
AfiI CCNNNNNNNGG 1 cut(s) 17
AluBI AGCT 1 cut(s) 182
AluI AGCT 1 cut(s) 182
Alw21I GWGCWC 1 cut(s) 145
Alw26I GTCTC 1 cut(s) 117
AlwI GGATC 1 cut(s) 33
AoxI GGCC 1 cut(s) 92
ApeKI GCWGC 4 cut(s) 182, 203, 341, 344
ApoI RAATTY 1 cut(s) 238
AspS9I GGNCC 1 cut(s) 82
AsuC2I CCSGG 1 cut(s) 351
AvaII GGWCC 1 cut(s) 82
BanII GRGCYC 1 cut(s) 275
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 145
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 4 cut(s) 169, 190, 328, 331
BceAI ACGGC 1 cut(s) 79
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BcnI CCSGG 1 cut(s) 351
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 1 cut(s) 291
BisI GCNGC 4 cut(s) 183, 204, 342, 345
BlsI GCNGC 4 cut(s) 184, 205, 343, 346
BmcAI AGTACT 1 cut(s) 404
Bme1390I CCNGG 1 cut(s) 351
Bme18I GGWCC 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 1 cut(s) 83
BmrFI CCNGG 1 cut(s) 351
BpiI GAAGAC 1 cut(s) 432
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 76, 95
Bsc4I CCNNNNNNNGG 1 cut(s) 17
BseDI CCNNGG 2 cut(s) 76, 95
BseGI GGATG 1 cut(s) 277
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMII CTCAG 2 cut(s) 105, 165
BseRI GAGGAG 2 cut(s) 62, 461
BseXI GCAGC 4 cut(s) 169, 190, 328, 331
BsgI GTGCAG 1 cut(s) 330
BshFI GGCC 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 145
BsiSI CCGG 2 cut(s) 350, 369
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 117
BsnI GGCC 1 cut(s) 94
Bso31I GGTCTC 1 cut(s) 117
Bsp1286I GDGCHC 2 cut(s) 145, 275
Bsp143I GATC 1 cut(s) 25
BspACI CCGC 1 cut(s) 171
BspANI GGCC 1 cut(s) 94
BspCNI CTCAG 2 cut(s) 106, 166
BspLI GGNNCC 1 cut(s) 83
BspPI GGATC 1 cut(s) 33
BspTNI GGTCTC 1 cut(s) 117
BssECI CCNNGG 2 cut(s) 76, 95
BssMI GATC 1 cut(s) 25
Bst4CI ACNGT 1 cut(s) 77
Bst6I CTCTTC 1 cut(s) 438
BstDEI CTNAG 3 cut(s) 114, 132, 174
BstDSI CCRYGG 2 cut(s) 76, 95
BstENI CCTNNNNNAGG 1 cut(s) 15
BstF5I GGATG 1 cut(s) 277
BstKTI GATC 1 cut(s) 28
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 1 cut(s) 25
BstMWI GCNNNNNNNGC 2 cut(s) 179, 188
BstSCI CCNGG 1 cut(s) 349
BstV1I GCAGC 4 cut(s) 169, 190, 328, 331
BstV2I GAAGAC 1 cut(s) 432
BsuRI GGCC 1 cut(s) 94
BtgI CCRYGG 2 cut(s) 76, 95
BtsCI GGATG 1 cut(s) 277
Cfr13I GGNCC 1 cut(s) 82
Csp6I GTAC 3 cut(s) 68, 159, 403
CviJI RGCY 6 cut(s) 94, 154, 182, 191, 203, 273
CviKI_1 RGCY 6 cut(s) 94, 154, 182, 191, 203, 273
CviQI GTAC 3 cut(s) 68, 159, 403
DdeI CTNAG 3 cut(s) 114, 132, 174
DpnI GATC 1 cut(s) 27
DpnII GATC 1 cut(s) 25
DraIII CACNNNGTG 1 cut(s) 353
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Eco24I GRGCYC 1 cut(s) 275
Eco31I GGTCTC 1 cut(s) 117
Eco47I GGWCC 1 cut(s) 82
EcoNI CCTNNNNNAGG 1 cut(s) 15
EcoT38I GRGCYC 1 cut(s) 275
FaiI YATR 4 cut(s) 195, 258, 269, 441
FalI AAGNNNNNCTT 6 cut(s) 112, 144, 191, 223, 417, 449
Fnu4HI GCNGC 4 cut(s) 183, 204, 342, 345
FokI GGATG 1 cut(s) 264
FriOI GRGCYC 1 cut(s) 275
Fsp4HI GCNGC 4 cut(s) 183, 204, 342, 345
FspBI CTAG 1 cut(s) 291
GluI GCNGC 4 cut(s) 183, 204, 342, 345
HaeIII GGCC 1 cut(s) 94
HapII CCGG 2 cut(s) 350, 369
HinfI GANTC 1 cut(s) 294
HpaII CCGG 2 cut(s) 350, 369
Hpy166II GTNNAC 1 cut(s) 324
Hpy188I TCNGA 2 cut(s) 86, 115
Hpy188III TCNNGA 1 cut(s) 29
Hpy8I GTNNAC 1 cut(s) 324
HpyAV CCTTC 3 cut(s) 46, 84, 138
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 2 cut(s) 185, 347
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 188
HpyF3I CTNAG 3 cut(s) 114, 132, 174
HpySE526I ACGT 1 cut(s) 70
Kzo9I GATC 1 cut(s) 25
LpnPI CCDG 4 cut(s) 122, 359, 363, 382
Lsp1109I GCAGC 4 cut(s) 169, 190, 328, 331
MaeI CTAG 1 cut(s) 291
MaeII ACGT 1 cut(s) 70
MaeIII GTNAC 1 cut(s) 71
MalI GATC 1 cut(s) 27
MboI GATC 1 cut(s) 25
MboII GAAGA 9 cut(s) 74, 99, 300, 303, 306, 309, 327, 437, 455
MhlI GDGCHC 2 cut(s) 145, 275
MluCI AATT 2 cut(s) 211, 238
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 7 cut(s) 11, 21, 40, 169, 297, 439, 440
MseI TTAA 2 cut(s) 210, 215
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 2 cut(s) 350, 369
MspR9I CCNGG 1 cut(s) 351
MwoI GCNNNNNNNGC 2 cut(s) 179, 188
NciI CCSGG 1 cut(s) 351
NdeII GATC 1 cut(s) 25
NlaIV GGNNCC 1 cut(s) 83
NmeAIII GCCGAG 1 cut(s) 278
PfeI GAWTC 1 cut(s) 294
PkrI GCNGC 4 cut(s) 184, 205, 343, 346
PspN4I GGNNCC 1 cut(s) 83
PspPI GGNCC 1 cut(s) 82
RsaI GTAC 3 cut(s) 69, 160, 404
RsaNI GTAC 3 cut(s) 68, 159, 403
SaqAI TTAA 2 cut(s) 210, 215
SatI GCNGC 4 cut(s) 183, 204, 342, 345
Sau3AI GATC 1 cut(s) 25
Sau96I GGNCC 1 cut(s) 82
ScaI AGTACT 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 351
SduI GDGCHC 2 cut(s) 145, 275
SetI ASST 4 cut(s) 73, 184, 420, 432
SinI GGWCC 1 cut(s) 82
SmlI CTYRAG 1 cut(s) 261
SmoI CTYRAG 1 cut(s) 261
Sse9I AATT 2 cut(s) 211, 238
SsiI CCGC 1 cut(s) 171
SspI AATATT 1 cut(s) 254
SspMI CTAG 1 cut(s) 291
StyD4I CCNGG 1 cut(s) 349
TaaI ACNGT 1 cut(s) 77
TaiI ACGT 1 cut(s) 73
TasI AATT 2 cut(s) 211, 238
TatI WGTACW 1 cut(s) 402
TfiI GAWTC 1 cut(s) 294
Tru1I TTAA 2 cut(s) 210, 215
Tru9I TTAA 2 cut(s) 210, 215
TseI GCWGC 4 cut(s) 182, 203, 341, 344
TspDTI ATGAA 4 cut(s) 264, 318, 351, 456
VpaK11BI GGWCC 1 cut(s) 82
XagI CCTNNNNNAGG 1 cut(s) 15
XapI RAATTY 1 cut(s) 238
XspI CTAG 1 cut(s) 291
ZrmI AGTACT 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.