RLG00000009804

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0015265
Physical Location & Seq
Forward (+)
58205558 .. 58206028
471 bp
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UTR
Exon/CDS
Intron
RLM00000009804

Sequence Viewer

Length: 471 bp
ATGGAAGACCCTCGCAAAGGGAAGGAAGAGAAGGCAGGCATAGAAGAAGTGCGTTATCGGGGCGTTCGGAGGAGGCCATGGGGGAAGTTTGCGTCGGAGATAAGAGACCCTTCGAGGCAGGGTGCTCGTCTGTGGCTTGGTACATTCGACACAGCAGAGGAAGCTGCAAGGGCTTACGATAAGGCTGCTTTTGACCTAAGGGGTCATCTTGCCATACTCAACTTCCCCAATGAGTACTATTCTCGAGTCATGGGATCTCCTCCTTATCCTCCGAGATTCCAATCATCCGTTTCTTCTTCATCGTCTTCTTCTGCTTCGGCTGCAGCTACTGCTAGTAGAGGAGCTGGTGGGAGCTTTGAGAGTGGAACCAGCTCCTCTGCTGGACAGGGAAGGCAAGTGTTTGAGTTCGAGTATTTGGATGACAAGGTGTTGGAGGATCTTCTTGATTGGGAAGAGGAGAAACGTAAATGA

Protein Analysis

157

Amino Acids

17.38

Weight (kDa)

5.97

Isoelectric Point (pI)

65.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 18 - 69 1.4e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23230
fragaria_vesca FvH4_4g03460
malus_domestica MD13G1212900.v1.1 MD16G1216500.v1.1
prunus_persica Prupe.1G037800_v2.0.a1
pyrus_communis pycom13g18470 pycom16g18110
rosa_chinensis RchiOBHm_Chr4g0392461
rosa_laevigata RLG00000009804
rosa_multiflora Rmu_sc0004216.1_g000004 Rmu_sc0041365.1_g000001
rosa_roxburghii Rroxscaffold_5G00337720
rosa_rugosa Rorug03G0339000
rosa_samantha Rh4BG038100 Rh4DG040100
rosa_wichuraiana Rw4G003370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 262, 444
AfaI GTAC 2 cut(s) 142, 236
AfiI CCNNNNNNNGG 1 cut(s) 17
AluBI AGCT 5 cut(s) 164, 326, 344, 354, 372
AluI AGCT 5 cut(s) 164, 326, 344, 354, 372
Alw21I GWGCWC 1 cut(s) 127
Alw26I GTCTC 1 cut(s) 99
AlwI GGATC 2 cut(s) 262, 444
AlwNI CAGNNNCTG 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 243
AoxI GGCC 1 cut(s) 74
ApeKI GCWGC 4 cut(s) 164, 185, 320, 323
AvaI CYCGRG 1 cut(s) 243
AxyI CCTNAGG 1 cut(s) 197
BbsI GAAGAC 2 cut(s) 12, 297
Bbv12I GWGCWC 1 cut(s) 127
BbvI GCAGC 4 cut(s) 151, 172, 307, 335
BcgI CGANNNNNNTGC 4 cut(s) 107, 141, 167, 201
BcoDI GTCTC 1 cut(s) 99
BfaI CTAG 1 cut(s) 333
BfmI CTRYAG 1 cut(s) 321
BisI GCNGC 4 cut(s) 165, 186, 321, 324
BlsI GCNGC 4 cut(s) 166, 187, 322, 325
BmcAI AGTACT 1 cut(s) 236
BmeT110I CYCGRG 1 cut(s) 243
BmiI GGNNCC 1 cut(s) 367
BpiI GAAGAC 2 cut(s) 12, 297
BsaBI GATNNNNATC 1 cut(s) 280
BsaI GGTCTC 1 cut(s) 99
BsaJI CCNNGG 1 cut(s) 77
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse21I CCTNAGG 1 cut(s) 197
Bse8I GATNNNNATC 1 cut(s) 280
BseDI CCNNGG 1 cut(s) 77
BseGI GGATG 2 cut(s) 284, 424
BseJI GATNNNNATC 1 cut(s) 280
BseLI CCNNNNNNNGG 1 cut(s) 17
BseRI GAGGAG 5 cut(s) 85, 249, 354, 364, 470
BseXI GCAGC 4 cut(s) 151, 172, 307, 335
BshFI GGCC 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 127
BsiHKCI CYCGRG 1 cut(s) 243
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 99
BsnI GGCC 1 cut(s) 76
Bso31I GGTCTC 1 cut(s) 99
BsoBI CYCGRG 1 cut(s) 243
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 2 cut(s) 254, 436
Bsp19I CCATGG 1 cut(s) 77
BspANI GGCC 1 cut(s) 76
BspLI GGNNCC 1 cut(s) 367
BspMAI CTGCAG 1 cut(s) 325
BspPI GGATC 2 cut(s) 262, 444
BspTNI GGTCTC 1 cut(s) 99
BssECI CCNNGG 1 cut(s) 77
BssMI GATC 2 cut(s) 254, 436
BssT1I CCWWGG 1 cut(s) 77
Bst6I CTCTTC 2 cut(s) 21, 447
BstAPI GCANNNNNTGC 1 cut(s) 329
BstC8I GCNNGC 1 cut(s) 37
BstDEI CTNAG 1 cut(s) 197
BstDSI CCRYGG 1 cut(s) 77
BstENI CCTNNNNNAGG 1 cut(s) 15
BstF5I GGATG 2 cut(s) 284, 424
BstKTI GATC 2 cut(s) 257, 439
BstMAI GTCTC 1 cut(s) 99
BstMBI GATC 2 cut(s) 254, 436
BstMWI GCNNNNNNNGC 4 cut(s) 161, 170, 320, 329
BstSFI CTRYAG 1 cut(s) 321
BstV1I GCAGC 4 cut(s) 151, 172, 307, 335
BstV2I GAAGAC 2 cut(s) 12, 297
BstX2I RGATCY 2 cut(s) 254, 436
BstYI RGATCY 2 cut(s) 254, 436
Bsu36I CCTNAGG 1 cut(s) 197
BsuRI GGCC 1 cut(s) 76
BtgI CCRYGG 1 cut(s) 77
BtsCI GGATG 2 cut(s) 284, 424
Cac8I GCNNGC 1 cut(s) 37
CaiI CAGNNNCTG 1 cut(s) 329
CseI GACGC 1 cut(s) 81
Csp6I GTAC 2 cut(s) 141, 235
CviAII CATG 2 cut(s) 78, 250
CviQI GTAC 2 cut(s) 141, 235
DdeI CTNAG 1 cut(s) 197
DpnI GATC 2 cut(s) 256, 438
DpnII GATC 2 cut(s) 254, 436
Eam1104I CTCTTC 2 cut(s) 21, 447
EarI CTCTTC 2 cut(s) 21, 447
Eco130I CCWWGG 1 cut(s) 77
Eco31I GGTCTC 1 cut(s) 99
Eco81I CCTNAGG 1 cut(s) 197
Eco88I CYCGRG 1 cut(s) 243
EcoNI CCTNNNNNAGG 1 cut(s) 15
EcoT14I CCWWGG 1 cut(s) 77
ErhI CCWWGG 1 cut(s) 77
FaeI CATG 2 cut(s) 81, 253
FaiI YATR 4 cut(s) 41, 79, 215, 251
FalI AAGNNNNNCTT 2 cut(s) 94, 126
FatI CATG 2 cut(s) 77, 249
Fnu4HI GCNGC 4 cut(s) 165, 186, 321, 324
FokI GGATG 2 cut(s) 271, 431
Fsp4HI GCNGC 4 cut(s) 165, 186, 321, 324
FspBI CTAG 1 cut(s) 333
GluI GCNGC 4 cut(s) 165, 186, 321, 324
HaeIII GGCC 1 cut(s) 76
HgaI GACGC 1 cut(s) 81
Hin1II CATG 2 cut(s) 81, 253
HinfI GANTC 2 cut(s) 246, 276
Hpy188I TCNGA 3 cut(s) 69, 97, 273
Hpy188III TCNNGA 2 cut(s) 243, 443
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 4 cut(s) 16, 25, 120, 384
HpyCH4IV ACGT 1 cut(s) 463
HpyCH4V TGCA 2 cut(s) 167, 323
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 170, 320, 329
HpyF3I CTNAG 1 cut(s) 197
HpySE526I ACGT 1 cut(s) 463
Hsp92II CATG 2 cut(s) 81, 253
Kzo9I GATC 2 cut(s) 254, 436
LmnI GCTCC 3 cut(s) 341, 351, 377
LpnPI CCDG 6 cut(s) 21, 104, 330, 366, 371, 382
Lsp1109I GCAGC 4 cut(s) 151, 172, 307, 335
MaeI CTAG 1 cut(s) 333
MaeII ACGT 1 cut(s) 463
MalI GATC 2 cut(s) 256, 438
MboI GATC 2 cut(s) 254, 436
MboII GAAGA 9 cut(s) 17, 38, 56, 285, 288, 297, 300, 431, 464
MflI RGATCY 2 cut(s) 254, 436
MhlI GDGCHC 1 cut(s) 127
MlyI GAGTC 1 cut(s) 255
MmeI TCCRAC 2 cut(s) 75, 411
MwoI GCNNNNNNNGC 4 cut(s) 161, 170, 320, 329
NcoI CCATGG 1 cut(s) 77
NdeII GATC 2 cut(s) 254, 436
NlaIII CATG 2 cut(s) 81, 253
NlaIV GGNNCC 1 cut(s) 367
PaeR7I CTCGAG 1 cut(s) 243
PfeI GAWTC 1 cut(s) 276
PkrI GCNGC 4 cut(s) 166, 187, 322, 325
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 367
PstI CTGCAG 1 cut(s) 325
PstNI CAGNNNCTG 1 cut(s) 329
PsuI RGATCY 2 cut(s) 254, 436
RsaI GTAC 2 cut(s) 142, 236
RsaNI GTAC 2 cut(s) 141, 235
SatI GCNGC 4 cut(s) 165, 186, 321, 324
Sau3AI GATC 2 cut(s) 254, 436
ScaI AGTACT 1 cut(s) 236
SchI GAGTC 1 cut(s) 255
SduI GDGCHC 1 cut(s) 127
SetI ASST 8 cut(s) 166, 198, 328, 346, 356, 374, 429, 466
SfcI CTRYAG 1 cut(s) 321
Sfr274I CTCGAG 1 cut(s) 243
SlaI CTCGAG 1 cut(s) 243
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
SspMI CTAG 1 cut(s) 333
StyI CCWWGG 1 cut(s) 77
TaiI ACGT 1 cut(s) 466
TaqI TCGA 4 cut(s) 113, 147, 244, 408
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 1 cut(s) 276
TseI GCWGC 4 cut(s) 164, 185, 320, 323
TspDTI ATGAA 1 cut(s) 288
TspGWI ACGGA 1 cut(s) 277
XagI CCTNNNNNAGG 1 cut(s) 15
XhoI CTCGAG 1 cut(s) 243
XspI CTAG 1 cut(s) 333
ZrmI AGTACT 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.